A culture tank for adventitious roots

Abstract

The application discloses a kind of adventitious root culture tanks, culture tank includes jar body, air inlet structure, cutting structure, the inside hollow of the jar body is set, for containing adventitious root and culture solution, the air inlet structure is at least set four groups in the bottom of the jar body, for the air supply to the inside of the jar body, the cutting structure is at least set two groups in the bottom of the jar body, for cutting adventitious root in culture solution, on the jar body, the cutting structure and the line of adjacent two air inlet structure installation positions approximately constitute isosceles triangle, the cutting structure installation position is higher than the air inlet structure in vertical direction;The application solves the problem that adventitious root in previous culture tank is connected, wound and the like due to improper cutting or uneven air supply.

Description

Technical Field

[0001] This invention belongs to the field of biological culture device technology, specifically, it relates to an adventitious root culture vessel. Background Technology

[0002] In the field of tissue and cell culture, some plant tissues and cells have been shown to have effects such as anti-fatigue, anti-aging, regulation of the central nervous system, enhancement of immunity, improvement of insufficient blood supply to the heart and brain, and inhibition of tumor cell production. In recent years, ginseng and adventitious roots have been widely used in various cosmetics, health products, and beverages, with a very broad market prospect.

[0003] Currently, due to over-harvesting and environmental destruction, wild ginseng resources are almost extinct, and field cultivation is the main source of ginseng. However, ginseng grows slowly, has a long cultivation period, and requires strict environmental conditions. Its quality is easily affected by climate, cultivation conditions, and pests and diseases. Complex cultivation techniques, excessive pesticide residues, and old ginseng fields also greatly limit the development prospects of artificially cultivated ginseng.

[0004] In the existing technology, various culture vessels have been designed for tissue and cell culture, especially suitable for adventitious root culture, and have been applied in the field of tissue and cell culture. To control the length of adventitious roots and prevent them from tangling, they need to be frequently trimmed. Typically, a rotating shaft with a blade fixed to one end is inserted into the vessel, and a motor is connected to the shaft and drives it to rotate, thereby using the high-speed rotation of the blade to cut the adventitious roots.

[0005] However, this cutting method cannot guarantee the cutting effect on adventitious roots. Some adventitious roots are not completely cut after contact with the blade and will rotate with the blade, causing the adventitious roots to become entangled in the blade. Therefore, it is necessary to frequently clean the adventitious roots that are entangled in the blade, which greatly reduces the efficiency of cutting adventitious roots and even affects the environment inside the culture tank, leading to a decrease in the quality and yield of adventitious roots.

[0006] In addition, air needs to be introduced into the tank through an air intake device. Existing technologies have achieved uniform air intake into the tank, but the specific structure and arrangement of the air intake device are not described.

[0007] In view of this, the present invention is hereby proposed. Summary of the Invention

[0008] The purpose of this invention is to overcome the shortcomings of the prior art and provide a tissue and cell culture vessel, especially a culture vessel more suitable for adventitious roots. It has a cutting structure that can cut the adventitious roots and an air intake structure that can introduce sterile air into the vessel. The relative positions of the cutting structure and the air intake structure installed at the bottom of the vessel can better control the root length of the adventitious roots and avoid the roots from being too long and dense, which would prevent the adventitious roots from absorbing nutrients and water normally.

[0009] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is as follows:

[0010] An adventitious root culture vessel includes: a vessel body for holding adventitious roots and culture medium;

[0011] The air intake structure includes at least four sets located at the bottom of the tank for supplying air into the tank.

[0012] The cutting structure includes at least two sets located at the bottom of the tank for cutting adventitious roots in the culture medium;

[0013] The discharge port is located at the bottom of the tank and is used for discharging materials;

[0014] On the tank body, the line connecting the cutting structure and the installation positions of the two adjacent air intake structures approximately forms an isosceles triangle, and the installation position of the cutting structure is higher than the air intake structure in the vertical direction.

[0015] Furthermore, the cutting structures are symmetrically arranged on both sides of the bottom sidewall of the tank with the discharge port at the bottom of the tank as the center, and the distance between the installation positions of the two sets of cutting structures and the discharge port is equal.

[0016] Furthermore, the air intake structure (2) is evenly arranged inside the bottom side wall of the tank and the four sets of air intake structures are equally spaced. The air intake structure's vent rod is kept horizontal and has a certain length. The vent rod is long and cylindrical, hollow inside for air intake, and the cavity wall is covered with micropores for air outlet. The projections of any two opposing sets of vent rods inside the tank are approximately parallel, and the projections of adjacent sets of vent rods inside the tank are approximately perpendicular.

[0017] Furthermore, the cutting structure and the air intake structure are located at different heights inside the bottom side wall of the tank. A cutting structure is provided between every two sets of air intake structures. The cutting structure forms an angle greater than 0° and less than 120° with the air vent of the air intake structure on the central axis.

[0018] Preferably, the cutting effect is good when the included angle is between 30° and 90°.

[0019] Furthermore, the cutting structure includes:

[0020] The shearing section is used to cut adventitious roots into small segments with a length of 5-15 mm.

[0021] The power unit is used to drive the shearing unit to work;

[0022] A spacer is used to connect the transmission unit and the power unit;

[0023] The shearing section includes a blade assembly, which is perpendicular to the central axis of the cutting structure; the blade assembly and the extension line of the venting rod projected into the tank form an angle greater than 0° and less than 90°.

[0024] Furthermore, the blade assembly is spaced upward along the central axis of the shearing section; the blade assembly includes a first blade and a second blade, the second blade rotating relative to the first blade to form a continuously opening and closing slit.

[0025] Furthermore, the spacer protrudes from the tank wall into the tank body along the vertical direction of the tank bottom sidewall and forms a groove on the outer side of the tank wall. The middle part of the spacer is flush with the tank bottom sidewall. The shearing part is connected to the outer convex surface of the spacer. The power part is installed in the groove.

[0026] Furthermore, the intake structure includes:

[0027] A gas guide bracket is used to uniformly supply gas into the tank.

[0028] A ventilation rod, located at one end of the air guide support, is used to deliver sterile air inside the air guide support to the inside of the tank in the form of bubbles;

[0029] In the four evenly distributed intake structures, the intake sections of two adjacent intake structures are connected to the intake tee pipe via intake branch pipes. The intake tee pipe is connected to the intake manifold and supplies air to both intake structures. The other two groups have the same connection method.

[0030] Furthermore, the air guide bracket is bent to form multiple straight sections extending at different angles. The air guide bracket includes: a first straight section, a second straight section, and a third straight section. The first straight section and the second straight section are arranged perpendicularly, and the second straight section and the third straight section are arranged at an obtuse angle. One end of the first straight section is provided with a flared opening, and the air rod is sleeved on the outer periphery of the third straight section. The first straight section and the second straight section, as well as the second straight section and the third straight section, are smoothly transitioned.

[0031] Furthermore, the bottom of the tank is provided with a tank bottom sidewall with an inverted conical structure, and mounting holes are provided on the tank bottom sidewall for mounting the air intake structure and the cutting structure.

[0032] Furthermore, the intake structure includes:

[0033] An air guide bracket is used to deliver sterile air into the tank.

[0034] A ventilation rod, located at one end of the air guide support, is used to deliver sterile air inside the air guide support to the inside of the tank in the form of bubbles;

[0035] The seat plate is fixedly connected to the air guide bracket.

[0036] The interface is located on the bottom side wall of the adventitious root culture vessel.

[0037] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.

[0038] 1. The adventitious root culture tank features a cutting structure on its inverted conical bottom sidewall, used to cut adventitious roots that have grown to a certain quantity or volume into small segments of 5-15mm in length. This ensures efficient transplantation of adventitious roots. The cutting structure includes a shearing section and a power section, with a spacer between them. This spacer allows the cutting structure to be installed on the inverted conical sidewall of the tank bottom, interacting with the air inlet structure to facilitate efficient transplantation of adventitious roots. The spacer also prevents bacteria or debris from entering the adventitious root culture tank through the cutting structure, ensuring a healthy environment inside the tank. This solves the problem of environmental contamination that easily occurs when cultivating adventitious roots using previous techniques, leading to unusable adventitious roots.

[0039] 2. Based on the growth environment requirements of adventitious roots, the culture tank has a cylindrical shape at one end and an inverted conical shape at the other end. An air intake structure is provided at the bottom of the inverted conical shape of the culture tank. The air intake structure is equipped with an air guide support and an air vent. The air guide support of the air intake structure is bent to form multiple straight sections extending at different angles. The air vent is located at one end of the air guide support. Sterile air is delivered into the tank from different angles through the air intake structure. The sterile air drives the flow of the culture medium in the tank. When the air vent is kept horizontal relative to the tank, the air outlet on the surface of the air vent is more uniform and the bubbles are more dense. This allows for better contact between the adventitious roots and the culture medium, promoting the growth of the adventitious roots.

[0040] 3. The bottom of the culture tank is designed with an inverted conical shape, which reduces the pressure of the nutrient solution on the adventitious roots and promotes their growth. When the cutting structure and the air intake structure work simultaneously, the culture solution inside the tank forms a turbulent vortex, which allows the adventitious roots suspended in the tank to have better contact with the nutrient solution.

[0041] The specific embodiments of the present invention will now be described in further detail with reference to the accompanying drawings. Attached Figure Description

[0042] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0043] Figure 1This is a schematic diagram of the overall structure of the culture vessel of the present invention;

[0044] Figure 2 This is a top view of the air intake structure and cutting structure of the present invention located at the bottom of the tank;

[0045] Figure 3 This is a schematic diagram of the cutting structure of the present invention located on the bottom side wall of the can;

[0046] Figure 4 This is a schematic diagram of the air intake structure of the present invention located on the bottom side wall of the tank;

[0047] Figure 5 This is a schematic diagram of the air inlet structure of an adventitious root culture vessel according to the present invention;

[0048] Figure 6 This is a schematic diagram of the cut structure of an adventitious root culture vessel according to the present invention;

[0049] Figure 7 This is a schematic diagram of the shearing section of the cutting structure of the present invention;

[0050] Figure 8 This is a schematic diagram of the connection between the air intake structure and the air intake pipeline of the present invention.

[0051] In the diagram: 1. Tank body; 2. Air inlet structure; 4. Tank cover; 5. Discharge port; 6. Cutting structure; 11. Tank bottom sidewall; 21. Air guide support; 211. First straight section; 212. Second straight section; 213. Third straight section; 22. Air inlet; 23. Seat plate; 24. Ventilation rod; 51. Interface; 31. Spacer; 32. Shearing section; 322. Blade assembly; 323. First blade; 324. Second blade; 33. Transmission section; 34. Power section; A1106. Air inlet branch pipe; A1107. Air inlet branch pipe; B8. Air inlet tee pipe.

[0052] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0053] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0054] In the description of this invention, it should be noted that the terms "inner" and "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0055] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," "contact," and "communication" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0056] like Figure 1 , 2 As shown, this embodiment discloses an adventitious root culture vessel, including a vessel body 1, a vessel lid 4, and a discharge port 5. The top of the vessel body 1 has a portion of the vessel lid 4 in a semi-spherical shape, the middle of the vessel body 1 has a cylindrical shape, and the bottom of the vessel body 1 has an inverted conical shape. The inverted conical portion gradually tapers from the side wall of the vessel body 1 towards the bottom discharge port 5, and the vessel body 1 is hollow. The inverted conical bottom of the vessel body 1 has an air inlet structure 2 for supplying sterile air into the vessel body 1 and a cutting structure 6 for shearing the adventitious roots inside the vessel body 1. The side wall 11 of the bottom of the vessel body 1 has mounting holes for installing the air inlet structure 2 and mounting holes for passing through the spacer 31 of the cutting structure 6; the two types of mounting holes have different sizes.

[0057] On the tank body 1, the line connecting the installation positions of the cutting structure 6 and the two adjacent air intake structures 2 approximately forms an isosceles triangle, and the installation position of the cutting structure 6 is higher than that of the air intake structure 2 in the vertical direction.

[0058] The mounting positions of the air intake structure 2 mounting hole and the cutting structure 6 mounting hole provided at the bottom side wall 11 of the tank approximately form an isosceles triangle. The positions of the two sets of cutting structure 6 mounting holes are on the same horizontal plane relative to the central axis of the tank body 1, and the mounting position of the cutting structure 6 is higher than the mounting position of the air intake structure 2.

[0059] The air intake structure 2 uniformly delivers sterile air or steam into the tank 1.

[0060] like Figure 3As shown, an adventitious root culture vessel includes a cutting structure 6 with a spacer 31. A circular mounting hole is formed in the bottom sidewall 11 of the culture vessel. The outer circumferential surface of the spacer 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is detachably installed in the mounting hole on the bottom sidewall 11. One side of the spacer 31 is connected to a shearing part 32, and the other side is connected to a power unit 34, separating the shearing part 32 and the power unit 34 inside and outside the adventitious root culture vessel. The shearing part 32 is installed on the side of the spacer 31 facing inwards from the vessel body 1, and the power unit 34 is installed on the side of the spacer 31 facing outwards from the adventitious root culture vessel. The power unit 34 drives the shearing part 32 to cut the adventitious roots. The cross-section of the shearing part 32 is smaller than the cross-section of the spacer 31, allowing the cutting structure 6 to be easily and quickly installed on the adventitious root culture vessel.

[0061] The cutting structure 6 forms an angle greater than 0° and less than 120° with the vent rod 24 of the air intake structure 2 on the central axis. Preferably, the cutting effect is better when the angle is between 30° and 90°.

[0062] like Figures 6-7 As shown, the shearing section 32 of the cutting structure 6 of an adventitious root culture vessel includes at least two sets of blade groups 322 connected in series on a rotating shaft. The blade groups 322 are evenly distributed along the axial direction of the rotating shaft, forming a multi-layer structure. Each blade group 322 is composed of a first blade 323 and a second blade 324 fitted together in pairs.

[0063] Specifically, all the first blades 323 in the blade assembly 322 are parallel to each other and fixed to the rotating shaft. The second blade 324 rotates relative to the first blades 323 to form a continuously opening and closing slit. When the second blade 324 is driven to rotate relative to the first blade 323, adjacent blade assemblies 322 simultaneously cut the adventitious roots entering the shearing section 32. The gap between each blade assembly 322 is 1.5 cm.

[0064] The shearing section 32 includes a blade assembly 322, which is perpendicular to the central axis of the cutting structure 6; the blade assembly 322 and the venting rod 24 form an angle greater than 0° and less than 90° between their extended projections inside the tank 1.

[0065] like Figure 6 As shown, the cutting structure 6 also includes a transmission section 33 between the shearing section 32 and the power section 34. Specifically, the transmission section 33 is cylindrical and is located inside the spacer 31 near one end of the shearing section 32. The outer surface of the inner cylinder of the transmission section 33 and the inner surface of the outer periphery of the spacer 31 can be slidably connected or rotatably connected and connected together by a bearing.

[0066] like Figures 2-3As shown, the bottom sidewall 11 of an adventitious root culture tank of the present invention is provided with at least two sets of cutting structures 6. The two sets of cutting structures 6 are symmetrically arranged on both sides of the bottom sidewall 11 with the discharge port 5 at the bottom of the tank body 1 as the center, and the distance between the installation positions of the two sets of cutting structures 6 and the discharge port 5 is equal. One end of the cutting structure 6 with the power unit 34 extends outward to the outside of the tank body 1, and one end with the shearing part 32 extends inward toward the central axis of the tank body 1, and is nearly perpendicular to the position formed with the bottom sidewall 11. The cutting structure 6 is provided on the bottom sidewall 11 to cut the adventitious roots that have grown to a certain number or a certain volume to a length suitable for secondary culture of adventitious roots. Preferably, the adventitious roots are cut into small segments of 5-15 mm.

[0067] like Figures 4-5 As shown, this embodiment discloses an installation assembly for an air intake structure 2 of an adventitious root culture tank. The air intake structure 2 is detachably connected to the bottom side wall 11 of the adventitious root culture tank. The installation assembly for the air intake structure 2 includes a base plate 23 and an interface 51. The interface 51 is formed on the bottom side wall 11 of the adventitious root culture tank. The air intake structure 2 is fixedly connected to the base plate 23. By rotating the base plate 23 relative to the interface 51 of the culture tank, the relative position of the ventilator 24 of the air intake structure 2 in the culture tank can be adjusted. This allows the air intake structure 2 to have more relative positions and orientations relative to the culture tank, improving the flexibility of the installation and setting of the air intake structure 2, and also helping to fully utilize the ventilation function of the air intake structure 2 in the culture tank.

[0068] The air intake structure 2 includes an air intake section 22, an air vent 24, and an air guide bracket 21 connecting the air intake section 22 and the air vent 24. It also includes a seat plate 23 that can be embedded in the interface 51. The seat plate 23 is sleeved on the outer periphery of the air guide bracket 21 and its edge is connected to the edge of the interface 51, separating the air vent 24 from the air intake section 22 inside and outside the adventitious root culture tank.

[0069] like Figure 5 As shown, the air intake structure 2 includes an air guide bracket 21, which is a bent pipe comprising a first straight section 211, a second straight section 212, and a third straight section 213. The first straight section 211 is perpendicular to the second straight section 212, and the second straight section 212 and the third straight section 213 are set at an obtuse angle, with a smooth transition between the first straight section 211 and the second straight section 212, and between the second straight section 212 and the third straight section 213. One end of the first straight section 211 is provided with an air intake part 22 for conveying sterile air. A venting rod 24 is fitted around the outer periphery of the third straight section 213. Gas enters the air guide bracket 21 from the air intake part 22 of the first straight section 211 of the air intake structure 2, and then passes sequentially through the second straight section 212 and the third straight section 213 into the venting rod 24. Sterile air is then conveyed into the tank 1 through the micropores on the surface of the venting rod 24. The air intake effect is optimal when the venting rod 24 is in a horizontal position.

[0070] The ventilation rod 24 is hollow inside, and its surface is densely covered with numerous micropores made of a material that can be sintered to a pore size of 1-20 μm. The micropores are spaced apart and extend from the inner to the outer surface of the ventilation rod 24, ensuring uniform airflow. The ventilation rod 24 extends from one end of the third straight section 213 of the air guide bracket 21 towards the central axis of the tank body 1. The line connecting the projections of the four ventilation rods 24 extending towards the central axis of the tank body 1 is approximately a square. The optimal effect is achieved when the ventilation rods 24 extend to or beyond the central axis, allowing all four sets of ventilation rods 24 to simultaneously deliver sterile air into the tank.

[0071] like Figure 2 As shown, in this embodiment, the bottom sidewall 11 of an adventitious root culture tank is provided with at least four sets of air inlet structures 2. The four sets of air inlet structures 2 are evenly arranged inside the bottom sidewall 11 and are spaced at equal intervals. The air vents 24 of the air inlet structures 2 are kept horizontal and their length is greater than the diameter of the base plate 23. The projections of any two opposing sets of air vents 24 inside the tank 1 are approximately parallel, and the projections of two adjacent sets of air vents 24 inside the tank 1 are approximately perpendicular. When the air inlet structures 2 introduce sterile air into the tank 1, the gas flow force plays a better role in stirring the adventitious roots and culture medium inside the tank 1, and can provide sufficient sterile air to the tank 1 for the adventitious roots to breathe. A good supply of sterile air can promote the respiratory metabolism and root growth of adventitious roots.

[0072] The venting rod 24 is a long column with a hollow interior for air intake and the cavity wall is covered with micropores for air exhaust. One end of the venting rod 24 is spaced from the bottom wall, and the other end is tangent to or intersects the center line of the tank 1, so that the projection of the extension direction of the venting rod 24 inside the tank 1 is approximately parallel.

[0073] like Figure 8As shown, the air intake structure 2 is connected in pairs to an air intake manifold A1106. Taking one air intake manifold A1106 as an example, the extension distance of the air intake manifold A1106 is less than the maximum radius of the culture tank. After extending a certain distance, the air intake manifold A1106 bends towards the front of the main support frame and extends through the main support frame to the same plane as the central axis of the culture tank. This plane is parallel to the front of the main support frame, so that the two air intake manifolds A1106 are symmetrically located below the culture tank and close to the center of the bottom of the culture device. After extending a certain distance, the air intake manifold A1106 extends vertically upwards for a certain distance and then extends two air intake branch pipes A1107 to both sides through the air intake tee pipe B8. The two air intake branch pipes A1107... The distance of 07 is greater than the distance between the central axis of the culture tank and the air intake structure 2. It bends upward at an angle and extends to connect with the two air intake structures 2 on the same side of the bottom of the culture tank. Another air intake branch pipe A1106 is symmetrically distributed with it. The two air intake branch pipes A1107 on the symmetrical side are connected to the two air intake structures 2 on the same side. The angle between the upward bend of the pipe and the pipe extended through the tee pipe is less than 90°. It is connected to the four air intake structures 2 at the bottom of the culture tank through the air intake tee pipe B8. Air is evenly delivered to the culture tank through the air intake branch pipe A1107 extending from the air intake tee pipe B8, which greatly improves the contact surface between air and culture medium. The pipes of the extended frame are hidden under the tank body, making full use of space without affecting the layout of other pipes.

[0074] like Figure 2 As shown, in one embodiment of the present invention, at least four sets of air inlet structures 2 and at least two sets of cutting structures 6 are provided on the bottom sidewall 11 of an adventitious root culture tank. They are located at different heights inside the bottom sidewall 11 near the discharge port 3 and around the discharge port 3. A cutting structure 6 is provided at intervals between every two sets of air inlet structures 2. The installation position of a cutting structure 6 on the same side of the bottom sidewall 11 and the installation position of the two adjacent sets of air inlet structures 2 form an approximately isosceles triangle within the tank body 1. The installation position of the cutting structure 6 is higher than that of the air inlet structure 2 in the vertical direction.

[0075] The relative positions of the air intake structure 2 and the cutting structure 6 cause the flow of culture medium and adventitious roots in the tank 1 to form a vortex. This vortex is generated by the combined action of the cutting structure 6 cutting the adventitious roots and the upward movement of bubbles generated when sterile air is introduced by the air intake structure 2.

[0076] The air intake structure 2 provides upward-flowing gas into the tank 1 and generates downward turbulence in the culture medium inside the tank 1. The turbulence causes the disordered plant tissue to align in the same direction and transports the plant tissue to the cutting structure 6, where it enters the cut of the shearing device and is cut into small segments.

[0077] The introduction of sterile air into the tank 1 reduces the liquid density at the bottom of the tank 1. Air bubbles generated by the venting rod 24 disperse the adventitious roots at the bottom of the tank 1, preventing them from tangling or becoming intertwined. The air intake structure 2 provides an upward buoyancy as it supplies sterile air into the tank 1. This buoyancy causes the liquid at the bottom of the tank 1 to flow, raising the adventitious roots towards the middle of the tank 1. Once the adventitious roots reach a certain height, they naturally flow back to the bottom of the tank 1 due to gravity. This process causes the adventitious roots to tumble and churn within the tank 1. During this tumbling process, the adventitious roots are attracted by the attractive force generated by the rotation of the shearing section 32 of the cutting structure 6.

[0078] When the shearing part 32 in the cutting structure 6 rotates, the blade assembly 322 in the shearing part 32 will rotate rapidly. The fluid flow force generated by the rotation of the blade assembly 322 will create a low-pressure area around the cutting structure 6 and generate a high negative pressure around the shearing part 32. When the adventitious root approaches the shearing part 32, the adventitious root will be attracted by the shearing part 32 and sheared.

[0079] The installation method of air intake structure 2 and cutting structure 6 can better trim and maintain adventitious roots, provide sufficient sterile air for adventitious roots, and better control the length of adventitious roots, avoiding excessively long and dense adventitious root systems that are not conducive to material discharge and processing.

[0080] The air intake structure 2 and the cutting mechanism 6 are respectively arranged symmetrically with respect to the center of the tank body 1.

[0081] One end of the vent rod 24 of the air intake structure 2 is spaced from the bottom side wall 11 of the tank, and the other end is tangent to or intersects the center line of the tank body 1, so that the projection of the extension direction of the vent rod 24 inside the tank body 1 is approximately parallel.

[0082] The gas provided by the aeration rod 24 causes the culture medium and plant tissue in the tank 1 to circulate along a parabolic trajectory that rises near the central axis and falls near the inner wall of the tank.

[0083] The cutting mechanism 6 has a cut end that extends toward the center line of the tank and has a gap between the ends to allow the culture medium and plant tissue rising near the central axis to pass through.

[0084] As the culture medium rises and falls along a near-parabolic trajectory, it exerts a driving force on the plant tissue, causing it to align regularly in the same direction and flow downwards into the cut of the cutting structure 6.

[0085] The cutting structure 6 and the air intake structure 2 are configured in this way to provide uniformly distributed gas into the tank 1. Since the distribution ratio of the venting rod 24 at the center line of the tank 1 is higher than that at the bottom side wall 11, the kinetic energy provided by the gas at the center line area is greater than the potential energy and the pressure above the culture medium. The culture medium can move upward under the influence of the airflow. When it reaches a certain height, it is far away from the venting rod 24, and the kinetic energy generated by the gas on the culture medium weakens and becomes less than the potential energy and the pressure above the culture medium. At the same time, the potential energy and the pressure above the culture medium at the inner wall are greater than the kinetic energy provided by the gas. This allows the culture medium to rise along the area near the central axis, then diffuse to the periphery, and circulate along the trajectory of the outer parabolic surface that descends near the inner wall of the tank. The moving culture medium guides the plant tissue to arrange in a regular manner and flow into the cut of the cutting mechanism 6. At the same time, the rotation of the cutter in the cutting mechanism 6 generates vortex suction, which further draws the regularly arranged plant tissue into the cut of the cutting mechanism 6, ensuring the uniformity of the cut and preventing uneven cutting of entangled plant tissue or repeated cutting of plant tissue.

[0086] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An adventitious root culture vessel, characterized in that, The application relates to a device for culturing adventitious roots, comprising: a tank (1) for containing adventitious roots and culture solution; a discharge port (5) in the bottom of the tank (1) for discharging; cutting structures (6) arranged on the bottom of the tank (1) and symmetrically arranged on both sides of the tank bottom side wall (11) with the discharge port (5) in the bottom of the tank (1) as the center, used for cutting the adventitious roots in the culture solution; air inlet structures (2) arranged on the bottom of the tank (1) and used for supplying air to the inside of the tank (1); the air inlet structures (2) are provided with air supply rods (24) which are kept horizontal and have a certain length, the air supply rods (24) are long columnar and hollow for air inlet, and the cavity walls are provided with micropores for air outlet; the projections of any two opposite air supply rods (24) in the tank (1) are approximately parallel, and the projections of two adjacent air supply rods (24) in the tank (1) are approximately perpendicular; the cutting structures (6) and the air supply rods (24) of the air inlet structures (2) form an included angle between 30 DEG and 90 DEG on the central axis; one end of the cutting structures (6) with cutouts extends to the direction of the central line of the tank and has a gap between the end portions, so that the culture solution and plant tissues rising in the region close to the central axis can pass through; the air supplied by the air supply rods (24) makes the culture solution and plant tissues in the tank (1) circularly move along a trajectory of an outer parabolic surface type rising in the region close to the central axis and descending close to the inner wall of the tank; the culture solution generates a pushing force on the plant tissues during the rising and descending along the trajectory of the parabolic surface type, so that the plant tissues are regularly arranged in the same direction and flow downwards into the cutouts of the cutting structures (6); on the tank (1), the connecting lines of the cutting structures (6) and the adjacent two air inlet structures (2) are approximately isosceles triangles, and the mounting positions of the cutting structures (6) are higher than those of the air inlet structures (2) in the vertical direction.

2. The adventitious root culture tank according to claim 1, wherein one cutting structure (6) is arranged between every two air inlet structures (2), and the distances between the mounting positions of the two cutting structures (6) and the discharge port (5) are equal.

3. The adventitious root culture tank according to claim 1 or 2, wherein the air inlet structures (2) are uniformly arranged in the tank bottom side wall (11) and are equidistantly arranged between the four air inlet structures (2).

4. The rhizome culture tank according to claim 3, wherein the cutting structures (6) and the air inlet structures (2) are arranged at different heights in the tank bottom side wall (11), and one cutting structure (6) is arranged between every two air inlet structures (2).

5. The rhizome culture tank according to claim 2, wherein the cutting structure (6) comprises: a shearing part (32) for shearing the adventitious roots into small segments with a length of 5-15 mm; a power part (34) for driving the shearing part (32) to work; a spacer sleeve (31) for connecting the transmission part (33) and the power part (34); the shearing part (32) comprises a blade group (322) which is arranged perpendicularly to the central axis of the cutting structure (6).

6. A rhizotron tank according to claim 5, wherein The blade group (322) is arranged in the shearing part (32) in the axial direction upward; the blade group (322) comprises a first blade (323) and a second blade (324), the first blade (323) is parallel to each other and fixed with the rotating shaft, and the second blade (324) is rotated relative to the first blade (323) to form a continuously opening and closing cut.

7. The adventitious root culture tank of claim 5, wherein The spacer sleeve (31) protrudes from the tank wall to the inside of the tank body (1) along the vertical direction of the tank bottom side wall (11) and forms a groove on the outside of the tank wall, the middle part of the spacer sleeve (31) is flush with the tank bottom side wall (11), the shearing part (32) is connected with the outer convex surface of the spacer sleeve (31), and the power part (34) is installed at the groove.

8. The rhizome culture tank according to claim 3, wherein The air inlet structure (2) comprises: An air guide support (21) for uniformly supplying air to the inside of the tank body (1); A venting rod (24) arranged at one end of the air guide support (21) for delivering sterile air in the air guide support (21) to the inside of the tank body (1) in the form of bubbles; Four groups of the air inlet structure (2) are uniformly distributed, two adjacent groups of the air inlet structure (2) are connected through air inlet branch pipes (A1107) and air inlet three-way pipes (B8), the air inlet three-way pipes (B8) are connected with air inlet branch pipes (A1106) and supply air to the two groups of the air inlet structure (2), and the other two groups have the same connection mode; The venting rod (24) is hollow inside and has a large number of micropores densely arranged on the surface, is made of a material sintered into a pore size of 1-20um, and the micropores extend from the inner surface of the venting rod (24) to the outer surface of the venting rod (24) to make the venting of the venting rod (24) uniform.

9. A rhizotron tank according to claim 8, wherein The air guide support (21) is bent to form a plurality of straight sections extending at different angles, the air guide support (21) comprises a first straight section (211), a second straight section (212) and a third straight section (213), the first straight section (211) is arranged vertically to the second straight section (212), the second straight section (212) is arranged at an obtuse angle to the third straight section (213), one end of the first straight section (211) is provided with a bell mouth, the third straight section (213) is sleeved with the venting rod (24), and the first straight section (211) and the second straight section (212) and the second straight section (212) and the third straight section (213) are smoothly connected.

10. The rhizome culture tank according to claim 1, wherein The tank body (1) is provided with a tank bottom side wall (11) in the form of an inverted conical structure, and mounting holes are formed in the tank bottom side wall (11) for mounting the air inlet structure (2) and the cutting structure (6).

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