Use of a fungicidal composition containing metrafenone for the control of citrus anthracnose

Abstract

The application belongs to the technical field of pesticide fungicidal technology, and discloses a use of a fungicidal composition containing benzoic enofluoroquinazolinone for preventing and treating citrus anthracnose, wherein the fungicidal composition comprises active ingredient A and active ingredient B; the active ingredient A is benzoic enofluoroquinazolinone; the active ingredient B is prochloraz or a salt thereof; and the mass ratio of the active ingredient A to the active ingredient B is 40:1-1:50. The fungicidal composition has obvious synergistic effect for preventing and treating citrus anthracnose, can effectively control the spread and development of the disease, reduces the use amount of pesticides, is safe to crops, and is friendly to the environment.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of pesticide fungicides, and discloses a use of a fungicidal composition containing benzovindiflucarb for preventing and treating citrus anthracnose. BACKGROUND

[0002] Citrus is the world's first fruit tree species, and its planting area and yield are the largest. Citrus anthracnose caused by Colletotrichum gloeosporioides is one of the important diseases that occur universally in the world's citrus production areas.

[0003] In recent years, with the large-area planting of high-quality citrus, the occurrence and damage of citrus anthracnose have become increasingly serious. The disease has broken out and caused disaster in most citrus production areas, especially in Satsuma mandarin and lemon. The disease can infect various organs or parts of citrus such as leaves, branches, flowers and fruits, often causing flower drop, fruit drop, branch dieback, fruit rot during storage, and causing the citrus tree to weaken and reduce yield and income.

[0004] Benzovindiflucarb is a pyrazole amide fungicide developed by Syngenta, which belongs to succinate dehydrogenase inhibitors. It acts on the protein complex II in the electron transport chain of pathogen mitochondria, affects the electron transport system of the electron respiratory chain of the pathogen, hinders its energy metabolism, inhibits the growth of the pathogen, and leads to its death. Benzovindiflucarb has a broad fungicidal spectrum, high efficiency and long persistence. However, its action site is single, and the resistance development level is high. Therefore, the combination of benzovindiflucarb with other fungicides with different mechanisms of action is helpful for resistance management.

[0005] Prochloraz is an imidazole fungicide that has good control effect on diseases caused by ascomycetes and deuteromycetes. Its mechanism of action is to interfere with the biosynthesis of sterols in the cell wall of the pathogen, thereby playing a fungicidal role.

[0006] At present, benzimidazole and substituted benzene fungicides are mostly used to prevent and treat the disease, but because they have been used for many years in succession, citrus anthracnose has developed resistance to them, and the control effect has decreased significantly. Therefore, it is urgent to screen more fungicides for effectively preventing and treating citrus anthracnose. SUMMARY

[0007] Based on the above situation, the purpose of the present application is to provide a use of a fungicidal composition containing benzovindiflucarb for preventing and treating citrus anthracnose. The fungicidal composition has good control effect on citrus anthracnose, has the characteristics of long persistence and significant synergistic effect, and can significantly delay the development of pathogen resistance and reduce the dosage of the pesticide.

[0008] In order to achieve the above object, the application adopts the following technical scheme: a use of a bactericidal composition containing benzo enofluoro fungicide for preventing and treating citrus anthracnose, wherein the bactericidal composition comprises active ingredient A and active ingredient B, the active ingredient A is benzo enofluoro fungicide, the active ingredient B is prochloraz or a salt thereof, and the mass ratio of the active ingredient A to the active ingredient B is 40:1-1:50.

[0009] Further, the active B is prochloraz, prochloraz manganese salt or prochloraz copper salt.

[0010] Further, when the active ingredient B is prochloraz, the mass ratio of the active ingredient A to the active ingredient B in the bactericidal composition is 40:1-1:30.

[0011] When the active ingredient B is prochloraz manganese salt, the mass ratio of the active ingredient A to the active ingredient B in the bactericidal composition is 30:1-1:40.

[0012] When the active ingredient B is prochloraz copper salt, the mass ratio of the active ingredient A to the active ingredient B in the bactericidal composition is 20:1-1:50.

[0013] Further, when the active ingredient B is prochloraz, the mass ratio of the active ingredient A to the active ingredient B in the bactericidal composition is 30:1-1:20.

[0014] When the active ingredient B is prochloraz manganese salt, the mass ratio of the active ingredient A to the active ingredient B in the bactericidal composition is 25:1-1:20.

[0015] When the active ingredient B is prochloraz copper salt, the mass ratio of the active ingredient A to the active ingredient B in the bactericidal composition is 10:1-1:30.

[0016] Further, the total content of the active ingredient A and the active ingredient B in the pesticide composition is 0.5-80wt% based on 100wt% of the total weight of the bactericidal composition.

[0017] Further, the total content of the active ingredient A and the active ingredient B in the pesticide composition is 1-70wt% based on 100wt% of the total weight of the bactericidal composition.

[0018] Further, the bactericidal composition further comprises an auxiliary agent selected from one or more of wetting agent, dispersing agent, emulsifying agent, thickening agent, disintegrating agent, antifreezing agent, defoaming agent, solvent, preservative, stabilizer, synergist and carrier.

[0019] The wetting agent is selected from one or more of alkyl benzene sulfonate, alkyl naphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sodium sulfosuccinate, alpha olefin sulfonate, alkyl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkyl phenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, soap nut powder, soapberry powder, SOPA, detergent, emulsifier 2000 series, and wetting penetrant F; and / or

[0020] The dispersing agent is selected from one or more of lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, styryl phenol ethoxylate phosphate ester, fatty alcohol ethoxylate, alkyl phenol polyoxyethylene ether, alkyl phenol polyoxyethylene ether formaldehyde condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylate, phosphate, EO-PO block copolymer, and EO-PO graft copolymer; and / or

[0021] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkyl phenol formaldehyde resin polyoxyethylene ether, styrene phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styryl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkyl phenol ether phosphate ester; and / or

[0022] The thickening agent is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and white carbon black; and / or

[0023] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0024] The antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0025] The antifoaming agent is selected from one or more of C 10 -C 20 One or more of saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 Fatty alcohols; and / or

[0026] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethyl formamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0027] the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, carboxin and 1,2-benzisothiazolin-3-one; and / or

[0028] the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0029] the synergist is selected from phosphorus synergist, piperonyl butoxide; and / or

[0030] the carrier is selected from one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil and vegetable oil derivative.

[0031] The present application can enhance the efficacy, reduce the amount of drug used and lower the cost by optimizing the content of active ingredient and adjuvant in the fungicidal composition to achieve a better balance between toxicity and residue.

[0032] Further, the fungicidal composition of the present application can be diluted by the user before use or used directly. The preparation can be prepared by the processing method known to the person skilled in the art, i.e. mixing the active substance with liquid solvent or solid carrier, and then adding one or more of surfactants such as dispersant, stabilizer, wetting agent, binder, antifoaming agent, disintegrant, antifreeze agent, etc.

[0033] Further, the fungicidal composition of the present application can be processed into any pesticide-acceptable formulation form as needed, which is solid formulation or liquid formulation.

[0034] Further, the solid formulation includes powder, granule, pellet, tablet, strip, wettable powder, oil-dispersible powder, emulsion powder, water-dispersible granule, emulsion granule, water-dispersible tablet, soluble powder, soluble tablet or soluble granule.

[0035] The liquid formulation includes soluble liquid, soluble gel, oil, spreadable oil, emulsion, emulsion, dispersible liquid, paste, water emulsion, oil emulsion, microemulsion, lipid suspension, microcapsule suspension, oil suspension, dispersible oil suspension, suspoemulsion, microcapsule suspension-suspension, microcapsule suspension-water emulsion, microcapsule suspension-suspoemulsion or seed treatment suspension.

[0036] More preferably, the dosage form is suspension, wettable powder, water emulsion, emulsion, microemulsion, soluble liquid, seed treatment suspension or water-dispersible granule.

[0037] Further, the anthracnose of citrus is a disease caused by Colletotrichum gloeosporioides.

[0038] Further, the fungicidal composition and / or its preparation are applied to the medium where the disease or its growth needs to be controlled.

[0039] The present application has the following beneficial effects:

[0040] The fungicidal composition of the present application has excellent control effect on the anthracnose of citrus, significant synergistic effect, long effective period, safety to crops, effectively reduces the use amount of pesticides, and is friendly to the environment. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical scheme and advantages of the present application clearer, the present application is further described in detail below in combination with specific preparation examples and embodiments. It should be understood that the specific preparation examples and embodiments described herein are only used to explain the present application and do not limit the present application.

[0042] Preparation Example

[0043] Preparation Example 1: 20% benthivalfungicide · prochloraz wettable powder (1:3)

[0044] Formulation composition: 5% benthivalfungicide, 15% prochloraz, 2% sodium dodecyl sulfate, 5% alkyl naphthalene sulfonate, 6% poly naphthalene formaldehyde sulfonate sodium salt, 10% kaolin, 10% white carbon black, bentonite to make up the balance;

[0045] Preparation method: according to the proportion of the formula, the active ingredients and dispersants, wetting agents and fillers are mixed, uniformly stirred in the stirring kettle, and uniformly mixed by airflow crusher for several times. The production process control temperature is not more than 40℃, and the wettable powder of the present application composition can be prepared.

[0046] Preparation Example 2: 30% benthivalfungicide · prochloraz suspension concentrate (1:3)

[0047] Formulation composition: 7.5% benthivalfungicide, 22.5% prochloraz, 2% fatty alcohol polyoxyethylene ether, 6% alkyl aryl polyoxyethylene polyoxypropylene ether, 3% styrene phenol polyoxyethylene ether sulfate ester, 1% polycarboxylic acid sodium salt, 1.5% magnesium aluminum silicate, 0.1% carboxymethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0048] Preparation method: according to the proportion of the formula, the active ingredients benthivalfungicide and prochloraz, surfactants and other functional additives are placed in the reaction kettle in turn, mixed with water, sheared by high speed, wet sanding, and the production process control temperature is not more than 40℃. Finally, the suspension concentrate product is obtained by uniformity filtration.

[0049] Preparation Example 3: 14% benzo enofluazin · prochloraz emulsion in water (3:1)

[0050] Formulation composition: 10.5% benzo enofluazin, 3.5% prochloraz, 3% glycerol fatty acid ester polyoxyethylene ether, 3% polyoxyethylene sorbitan monooleate, 15% cyclohexanone, 0.1% xanthan gum, 0.1% isothiazolinone, 5% glycerol, 1.5% urea, 0.5% sodium sorbate, 0.1% silicone antifoaming agent, deionized water to make up the balance;

[0051] Preparation method: according to the formulation proportion of the example, the active ingredients are dissolved in the solvent and emulsifiers are added to make a uniform oil phase. Deionized water, antifreeze and the like are mixed together to form a uniform water phase. Under high-speed shearing, the oil phase is added to the water phase, and after the particle size is qualified, antifoaming agent, thickening agent and preservative are added and stirred uniformly to form a well-dispersed emulsion in water product.

[0052] Preparation Example 4: 24% benzo enofluazin · prochloraz emulsion oil (1:5)

[0053] Formulation composition: 4% benzo enofluazin, 20% prochloraz, 15% DMF, 14% styryl phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 20% propylene carbonate, xylene to make up the balance;

[0054] Preparation method: according to the formulation proportion, the metered benzo enofluazin and prochloraz, solvent, cosolvent are added to the blending kettle and stirred to dissolve, then emulsifiers are added, the remaining solvent is added to make up the balance, and stirring is carried out in the stirring kettle. After filtration, the required emulsion oil of the present application is obtained.

[0055] Preparation Example 5: 20% benzo enofluazin · prochloraz microemulsion (7:1)

[0056] Formulation composition: 17.5% benzo enofluazin, 2.5% prochloraz, 10% styryl phenol polyoxyethylene ether, 4% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% octylphenol polyoxyethylene ether phosphate, 1% sodium dodecyl sulfate, 2% xylene, 20% cyclohexanone, 3% ethylene glycol, 0.5% glycerol, deionized water to make up the balance;

[0057] Preparation method: according to the formulation proportion of the preparation example, the active ingredients, solvent and emulsifiers are mixed uniformly to prepare an oil phase, the antifreeze solution and water are mixed uniformly to prepare a water phase, the oil phase is added to the water phase under stirring, and stirring is carried out uniformly for 10 min, then silicone antifoaming agent is added and stirred uniformly to obtain small droplets with an oil phase particle size of 0.01-0.1 microns, thereby obtaining the microemulsion of the present application.

[0058] Preparation Example 6: 24% benzo enofluazin · prochloraz manganese salt wettable powder (1:1)

[0059] Formulation composition: 12% benzo ene fluazinam, 12% prochloraz manganese salt, 3% polycarboxylate sodium salt, 2% naphthalene sulfonate formaldehyde condensate, 2% Laka powder BX, 5% white carbon black, kaolin to make up the balance;

[0060] Preparation method: according to the formulation proportion, the effective ingredients and dispersants, wetting agents and fillers are mixed, uniformly stirred in the stirred tank, and uniformly mixed by airflow crusher for multiple times, so that the wettable powder of the present application composition is prepared.

[0061] Preparation example 7: 50% benzo ene fluazinam·prochloraz manganese salt water dispersible granules (1:3)

[0062] Formulation composition: 12.5% benzo ene fluazinam, 37.5% prochloraz manganese salt, 10% lignin sulfonate, 4% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 5% white carbon black, 30% starch, kaolin to make up the balance;

[0063] Preparation method: according to the formulation proportion of the example, the active ingredients are added to the carrier, and surfactants and other functional adjuvants are added therein, mixed, 10-25% water is added after airflow crushing, and then kneading, granulation, drying and screening are carried out to prepare the water dispersible granule product; or the crushed powder is sprayed with water in a fluidized bed granulator, granulated, dried, and then screened to prepare the product.

[0064] Preparation example 8: 27% benzo ene fluazinam·prochloraz manganese salt suspension concentrate (5:1)

[0065] Formulation composition: 22.5% benzo ene fluazinam, 4.5% prochloraz manganese salt, 1% isomeric tridecanol polyoxyethylene ether, 1% naphthalene sulfonate formaldehyde condensate, 3% styrene phenol polyoxyethylene ether phosphate, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0066] Preparation method: according to the formulation proportion, the active ingredients benzo ene fluazinam and prochloraz, surfactants and other functional adjuvants are sequentially placed in a reaction kettle, mixed with water, sheared at high speed, wet sand ground, and finally filtered to obtain the suspension concentrate product.

[0067] Preparation example 9: 36% benzo ene fluazinam·prochloraz copper salt suspension concentrate (1:2)

[0068] Formulation composition: 12% benzo ene fluazinam, 24% prochloraz copper salt, 3% alkyl phenol polyoxyethylene ether, 4% alkyl phenol polyoxyethylene ether phosphate, 1% polycarboxylate sodium salt, 0.25% xanthan gum, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0069] Preparation method: the same as Preparation Example 8.

[0070] Preparation Example 10: 30% benzo enofluoro fungicide·prochloraz copper salt water dispersible granules (1:5)

[0071] Formulation composition: 5% benzo enofluoro fungicide, 25% prochloraz copper salt, 10% lignin sulfonate, 4% polycarboxylic acid sodium salt, 2% sodium dodecyl sulfate, 5% white carbon black, 30% starch, kaolin to make up the balance;

[0072] Preparation method: the same as Preparation Example 7.

[0073] Indoor activity test

[0074] Example 1: Indoor combined effect test of benzo enofluoro fungicide and prochloraz or its salt on citrus anthracnose

[0075] Test basis: Test reference NY / T 1156.2-2006 "Pesticide indoor biological test guidelines fungicides Part 2: Inhibition of pathogenic fungal mycelial growth test plate method"; NY / T 1156.6-2006 "Pesticide indoor biological test guidelines fungicides Part 6: Combined effect determination of mixtures".

[0076] Test strain: Colletotrichum gloeosporioides, indoor pure culture.

[0077] Test agent: 96% benzo enofluoro fungicide technical, 97% prochloraz technical, 98% prochloraz manganese salt technical, 98% prochloraz copper salt technical, all provided by the Group R&D Center.

[0078] Agent preparation: The technical agent was first dissolved in acetone to prepare single-agent stock solution, and different ratios were designed according to the purpose of mixing and the activity of the agent. Each single agent and each group of mixed agents was prepared into the required series of mass concentration gradients.

[0079] Under sterile operating conditions, the pre-melted sterilized PDA medium was cooled to 50℃, and the drug solution and PDA medium were mixed evenly in a 9cm diameter culture dish using a pipette, and a drug-containing plate medium was prepared, with sterile water as a blank control. Each treatment was repeated 4 times.

[0080] The preserved pathogenic bacteria were activated, and under sterile operating conditions, a 6mm diameter sterilized punch was used to cut the fungus cake from the edge of the colony, and the fungus cake was inoculated in the center of the drug-containing plate medium using an inoculator, with the mycelium facing up. Each culture dish contained 1 fungus cake, and the dish was covered and placed in a 25℃ constant temperature incubator for culture.

[0081] Data statistics and analysis:

[0082] According to the growth of the fungus in the blank control dish, the mycelium growth of the pathogenic fungus was investigated. The colony diameter was measured by using the cross method. According to the investigation results, the mycelium growth inhibition rate of each treatment concentration on the target fungus was calculated, in percentage (%), and the calculation results were kept to two decimal places.

[0083] D = D1 - D2

[0084] In the formula:

[0085] D - colony growth diameter;

[0086] D1 - colony diameter;

[0087] D2 - fungus cake diameter.

[0088]

[0089] I - mycelium growth inhibition rate;

[0090] D0 - blank control colony growth diameter;

[0091] D t - fungicide treatment colony growth diameter.

[0092] Test statistics: the data were processed by using the method of probability value analysis. The DPS statistical analysis system was used for analysis, and the EC 50 value was calculated to evaluate the activity of the test fungicide on the biological test material.

[0093] The co-toxicity coefficient (CTC value) of the mixture was calculated by the following formula:

[0094]

[0095] In the formula:

[0096] ATI - measured toxicity index of the mixture;

[0097] S - EC 50 of the standard fungicide, in milligrams per liter (mg / L);

[0098] M - EC 50 of the mixture, in milligrams per liter (mg / L).

[0099] TTI = TI A * P A + TI B * P B

[0100] In the formula:

[0101] TTI - theoretical toxicity index of the mixture;

[0102] TI A Toxicity index of A agent;

[0103] P A Percentage of A agent in the mixture, in percentage (%);

[0104] TI B Toxicity index of B agent;

[0105] P B Percentage of B agent in the mixture, in percentage (%).

[0106]

[0107] In the formula:

[0108] CTC - Co-toxicity coefficient

[0109] ATI - Actual toxicity index of the mixture

[0110] TTI - Theoretical toxicity index of the mixture

[0111] The co-toxicity coefficient CTC of the combination is greater than or equal to 120, which shows synergistic effect; CTC is less than or equal to 80, which shows antagonistic effect; 80 < CTC < 120, which shows additive effect.

[0112] The test results are shown in the following table:

[0113] Table 1 Indoor bioactivity test results of the combination of benzene alkenyl triflumizole and prochloraz against Colletotrichum gloeosporioides

[0114]

[0115]

[0116] Table 2 Indoor bioactivity test results of the combination of benzene alkenyl triflumizole and prochloraz manganese salt against Colletotrichum gloeosporioides

[0117]

[0118] Table 3 Indoor bioactivity test results of the combination of benzene alkenyl triflumizole and prochloraz copper salt against Colletotrichum gloeosporioides

[0119]

[0120]

[0121] The results of the indoor bioactivity determination test of the above table (Table 1-3) show that reasonable compounding of benzovindiflupyr and prochloraz or its salt at a certain mass ratio shows excellent control effect on citrus anthracnose. When the mass ratio of benzovindiflupyr and prochloraz is 1:70, the CTC is less than 80, and the citrus anthracnose fungus shows antagonism; when the mass ratio is 70:1~50:1, 1:50~1:40, 80<CTC<120, the citrus anthracnose fungus shows additive effect; and when the mass ratio is 40:1~1:30, the CTC is greater than 120, showing synergistic effect.

[0122] When benzovindiflupyr and prochloraz manganese salt are compounded, the CTC is less than 80 when the mass ratio is 70:1, and the citrus anthracnose fungus shows antagonism; when the mass ratio is 50:1~40:1, 1:50~1:70, 80<CTC<120, the citrus anthracnose fungus shows additive effect; and when the mass ratio is 30:1~1:40, the CTC is greater than 120, showing synergistic effect.

[0123] When benzovindiflupyr and prochloraz copper salt are compounded, the CTC is less than 80 when the mass ratio is 50:1, and the citrus anthracnose fungus shows antagonism; when the mass ratio is 50:1~30:1, 1:70, 80<CTC<120, the citrus anthracnose fungus shows additive effect; and when the mass ratio is 20:1~1:50, the CTC is greater than 120, showing synergistic effect.

[0124] Field efficacy test

[0125] Example 2: Field efficacy test of benzovindiflupyr and prochloraz or its salt compound on citrus anthracnose

[0126] The test was carried out in citrus orchard in Nanyong Village, Yongfu Town, Yongfu County, Guangxi Zhuang Autonomous Region. The soil organic matter content of the test site was 2.5%, the water and fertilizer management was moderate, and the terrain was flat.

[0127] Test crop: sugar orange.

[0128] Test design: The test set 11 treatments, 4 replicates, and each test plot was arranged randomly in groups.

[0129] Test method: The test used Gongnong-16 type knapsack sprayer, used crown spraying, and sprayed the leaf surface and fruit dripping water. No other fungicides were used in the test plot.

[0130] The whole test process totaled 2 times of pesticide application, the first time of pesticide application was on May 6, 2022, and the second time of pesticide application was 10 days later.

[0131] Investigation method: The efficacy investigation was carried out 21 days after the last application, and the effects of each treatment on leaf anthracnose and fruit anthracnose were investigated.

[0132] Leaf and fruit anthracnose investigation: The leaf / fruit disease incidence was investigated 21 days after application. Two trees were taken from each plot, and 2 spring branches or 6 fruits were investigated at 5 points (east, south, west, north and center) of each tree. The disease was graded according to Table 4, and the disease index and control effect were calculated.

[0133] Table 4 Leaf and fruit disease grading method

[0134] Disease rating Leaf Fruit 0 No lesion No lesion 1 Lesion area < 5% of leaf area 1-2 lesions on fruit 3 Lesion area 6-15% of leaf area 3-4 lesions on fruit 5 Lesion area 16-25% of leaf area 5-6 lesions on fruit 7 Lesion area 26-50% of leaf area 7-8 lesions on fruit and peduncle 9 Lesion area > 50% of leaf area or defoliation Lesion area > 50% of fruit area

[0135] Efficacy calculation method:

[0136]

[0137]

[0138] Safety investigation: The test was investigated at irregular intervals, and it was found that the test agent and the control agent had no abnormal influence on the growth of citrus, no phytotoxicity was found, and no adverse influence on beneficial insects in the test area was found.

[0139] The field efficacy test results are shown in the following table:

[0140] Table 5 Field efficacy test results of the combination of benzene fluoroaminozole and prochloraz or salts thereof for controlling citrus anthracnose

[0141]

[0142] The test results show that the fungicidal composition of the application for preventing and treating citrus anthracnose has a significant control effect. It can effectively control the spread of citrus anthracnose when applied to prevent and treat citrus anthracnose.

[0143] Through indoor toxicity determination and field efficacy test, the combination of benzene fluoroaminozole and prochloraz or salts thereof of the application shows a good control effect on citrus anthracnose. The fungicidal composition or its preparation obtained by the combination of the application has a significant control effect, and has the characteristics of high efficiency, broad spectrum, low residue, long persistence, strong systemicity, etc. In the test, no phytotoxicity of the combined agent to crops was found, which shows that in the case of synergistic fungicidal effect of the obtained fungicidal composition or preparation, the production cost and use cost can be reduced, and the crops are safe.

[0144] Although the present application has been described in detail with particular references to illustrative embodiments thereof, it should be understood that modifications and / or improvements can be made to the present application which do not depart from the spirit and scope of the present application, and such modifications and / or improvements are intended to be included within the scope of the present application.

Claims

1. The use of a fungicide composition containing benzovindiflubenzuron for the control of citrus anthracnose, characterized in that, The bactericidal composition comprises active ingredient A and active ingredient B. Active ingredient A is benzalkonium chloride, and active ingredient B is prochloraz, prochloraz manganese salt, or prochloraz copper salt. When active ingredient B is prochloraz, the mass ratio of active ingredient A to active ingredient B in the bactericidal composition is 40:1 to 1:30; when active ingredient B is prochloraz manganese salt, the mass ratio of active ingredient A to active ingredient B in the bactericidal composition is 30:1 to 1:40; when active ingredient B is prochloraz copper salt, the mass ratio of active ingredient A to active ingredient B in the bactericidal composition is 20:1 to 1:

50. The citrus anthracnose is caused by *Colletotrichum discus* (…). Colletotrichum gloeosporioides Citrus anthracnose caused by ).

2. The use according to claim 1, characterized in that, When the active ingredient B is imazalil, the mass ratio of active ingredient A to active ingredient B in the bactericidal composition is 30:1 to 1:20; when the active ingredient B is imazalil manganese salt, the mass ratio of active ingredient A to active ingredient B in the bactericidal composition is 25:1 to 1:20; when the active ingredient B is imazalil copper salt, the mass ratio of active ingredient A to active ingredient B in the bactericidal composition is 10:1 to 1:

30.

3. The use according to claim 1, characterized in that, Based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 0.5 to 80 wt%.

4. The use according to claim 3, characterized in that, Based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 70 wt%.

5. The use according to claim 1, characterized in that, In addition to the active ingredient, the bactericidal composition also includes agriculturally permitted auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

6. The use according to claim 1, characterized in that, The bactericidal composition is prepared into any formulation permitted for pesticides, wherein the formulation is a solid or liquid formulation. The solid dosage forms are powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules. The liquid formulation is a soluble agent, a soluble gel, a film-spreading oil, an emulsion, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspension emulsion, or a seed-treated suspension.

7. The use according to claim 6, characterized in that, The solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a suspension, an emulsion, an emulsifiable concentrate, or a microemulsion.

8. The use according to any one of claims 1-7, characterized in that, The bactericidal composition and / or its formulation are applied to the disease that needs to be controlled or to the medium in which it grows.

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