A method for preparing polyethylene glycol modified soy protein isolate gel

Abstract

This invention relates to the field of functional materials technology and discloses a method for preparing polyethylene glycol-modified soy protein isolate gel, comprising: Step 1, crushing soybeans and collecting the crushed soybeans; Step 2, extracting protein from the crushed soybeans to obtain soy protein isolate; Step 3, modifying the soy protein isolate with polyethylene glycol to obtain modified soy protein isolate; Step 4, mixing the modified soy protein isolate, basalt powder, and corn stalk powder, then granulating and crushing to obtain soy protein isolate powder; Step 5, preparing the polyethylene glycol-modified soy protein isolate gel by combining the soy protein isolate powder, theophylline, resin, and liquid paraffin. The polyethylene glycol-modified soy protein isolate gel prepared by this invention can effectively adsorb and fix heavy metal ions from water, purify water quality, and reduce the risk of heavy metal wastewater to the ecosystem and human health.

Description

Technical Field

[0001] This invention relates to the field of functional materials technology, and in particular to a method for preparing polyethylene glycol-modified soy protein isolate gel. Background Technology

[0002] Wastewater containing heavy metal ions refers to wastewater containing high concentrations of heavy metal ions such as lead, cadmium, and chromium. This type of wastewater may originate from various industrial production processes, mining, smelting, electroplating, and chemical industries, or it may come from certain watersheds within urban drainage systems. Heavy metal ions pose serious hazards to the environment and ecosystems, and even threaten human health; therefore, treating wastewater containing heavy metal ions is an important environmental protection task.

[0003] Currently, methods for treating wastewater containing heavy metal ions include chemical precipitation, ion exchange, and adsorbent adsorption. Among these, adsorbent adsorption has become the most common method for treating heavy metal ions in wastewater due to its advantages such as strong adsorption capacity and simple process. However, adsorbent adsorption also has some disadvantages. For example, the selection of adsorbents needs to consider the type and concentration of the target heavy metal, and different heavy metals may require different adsorbents. Therefore, developing an adsorbent capable of adsorbing multiple heavy metal ions is of great significance for environmental protection, resource recovery, and sustainable economic development. Summary of the Invention

[0004] This invention proposes a method for preparing polyethylene glycol-modified soy protein isolate gel, comprising:

[0005] Step 1: Crush the soybeans and collect the crushed soybeans;

[0006] Step 2: Extract protein from the crushed soybeans to obtain soy protein isolate;

[0007] Step 3: Modify the soy protein isolate using polyethylene glycol to obtain modified soy protein isolate;

[0008] Step 4: Mix the modified soy protein isolate, basalt powder and corn stalk powder, then granulate and crush to obtain soy protein isolate powder;

[0009] Step 5: Prepare polyethylene glycol modified soy protein isolate gel by combining the soy protein isolate powder, theophylline, resin and liquid paraffin.

[0010] Furthermore, in step 1, the soybeans are crushed and the crushed soybeans are collected. Specifically, fresh soybeans are soaked in water for 1-1.5 hours and then freeze-dried to obtain freeze-dried soybeans. The freeze-dried soybeans are then crushed to obtain the crushed soybeans.

[0011] Furthermore, in step 2, the crushed soybeans are subjected to protein extraction to obtain soy protein isolate. Specifically, the crushed soybeans are soaked in a buffer solution to dissolve the soy protein isolate in the buffer solution, filtered to obtain a soy protein isolate-buffer solution mixture, and the extracted soy protein isolate-buffer solution mixture is concentrated to obtain the soy protein isolate.

[0012] Furthermore, the buffer solution is a glycine buffer solution.

[0013] Furthermore, in step 3, the soy protein isolate is modified with polyethylene glycol to obtain modified soy protein isolate. Specifically, the soy protein isolate and polyethylene glycol are mixed and reacted at 50-60°C for 20-30 minutes to obtain the modified soy protein isolate.

[0014] Furthermore, in step 4, the method for preparing the corn stalk powder is as follows: soaking corn stalks in an alkaline solution, filtering after soaking, and then subjecting them to high-temperature treatment, followed by crushing the high-temperature treated corn stalks to obtain corn stalk powder.

[0015] Furthermore, after the corn stalks are crushed, they are passed through an 80-100 mesh sieve to obtain corn stalk powder.

[0016] Furthermore, the weight ratio of the modified soy protein isolate, the basalt powder, and the corn stalk powder is 5-8:1:2-3.

[0017] Furthermore, in step 5, the soy protein isolate powder, theophylline, resin and liquid paraffin are used to prepare polyethylene glycol modified soy protein isolate gel. Specifically, the resin and liquid paraffin are mixed and heated to obtain a gel substrate, and the soy protein isolate powder and theophylline are mixed into the gel substrate to obtain the polyethylene glycol modified soy protein isolate gel.

[0018] Furthermore, the weight ratio of the soy protein isolate powder, theophylline, resin and liquid paraffin is 5-20:1-5:10-15:15-20.

[0019] Furthermore, the soy protein isolate powder and theophylline are mixed into the gel substrate and then heated to 80-100℃ and held for 50-60 minutes to obtain the polyethylene glycol modified soy protein isolate gel.

[0020] Furthermore, the resin is a glycerol epoxy resin.

[0021] The present invention also provides a polyethylene glycol modified soy protein isolate gel, wherein the polyethylene glycol modified soy protein isolate gel is prepared by any of the preparation methods described above.

[0022] The present invention also provides an application of the polyethylene glycol modified soy protein isolate gel, including the application of the polyethylene glycol modified soy protein isolate gel in the adsorption of heavy metals;

[0023] And / or, the application of the polyethylene glycol-modified soy protein isolate gel in the preparation of heavy metal adsorption products.

[0024] Compared with existing technologies, its advantages are as follows:

[0025] This invention provides a method for preparing polyethylene glycol-modified soy protein isolate gel. The prepared polyethylene glycol-modified soy protein isolate gel can be used to treat heavy metal pollutants in wastewater. Specifically, the polyethylene glycol-modified soy protein isolate gel can effectively adsorb and fix heavy metal ions from water, purify water quality, and reduce the risk of heavy metal wastewater to the ecosystem and human health.

[0026] Many types of wastewater contain multiple heavy metal ions simultaneously. Adsorbents targeting a single heavy metal are less effective when dealing with complex wastewater. The polyethylene glycol-modified soy protein isolate gel disclosed in this invention, as an adsorbent capable of adsorbing multiple heavy metals, can better adapt to these complex scenarios, improve the overall efficiency of wastewater treatment, reduce the emission of multiple pollutants, and thus reduce the risks to ecosystems and human health. At the same time, the polyethylene glycol-modified soy protein isolate gel disclosed in this invention can also reduce environmental protection costs and reduce investment and maintenance costs for enterprises. Detailed Implementation

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Example 1

[0029] Step 1: Soak fresh soybeans in water for 1.5 hours and then freeze-dry them to obtain freeze-dried soybeans. Crush the freeze-dried soybeans to obtain crushed soybeans.

[0030] Step 2: Soak crushed soybeans in glycine buffer solution to dissolve soy protein isolate in the buffer solution. Filter to obtain soy protein isolate-buffer solution mixture. Concentrate the extracted soy protein isolate-buffer solution mixture to obtain soy protein isolate.

[0031] Step 3: Mix soy protein isolate and polyethylene glycol, and react at 60°C for 30 minutes to obtain modified soy protein isolate.

[0032] Step 4: Mix the modified soy protein isolate, basalt powder and corn stalk powder in a weight ratio of 8:1:3, then granulate and crush to obtain soy protein isolate powder.

[0033] Step 5: Mix glycerol epoxy resin and liquid paraffin at a weight ratio of 15:20 and heat to obtain a gel base. Mix soy protein isolate powder and theophylline at a weight ratio of 20:5 into the gel base and heat to 100°C for 60 minutes to obtain polyethylene glycol modified soy protein isolate gel.

[0034] Example 2

[0035] Step 1: Soak fresh soybeans in water for 1 hour and then freeze-dry them to obtain freeze-dried soybeans. Crush the freeze-dried soybeans to obtain crushed soybeans.

[0036] Step 2: Soak crushed soybeans in glycine buffer solution to dissolve soy protein isolate in the buffer solution. Filter to obtain soy protein isolate-buffer solution mixture. Concentrate the extracted soy protein isolate-buffer solution mixture to obtain soy protein isolate.

[0037] Step 3: Mix soy protein isolate and polyethylene glycol, and react at 50°C for 20 minutes to obtain modified soy protein isolate.

[0038] Step 4: Mix the modified soy protein isolate, basalt powder and corn stalk powder in a weight ratio of 5:1:2, then granulate and crush to obtain soy protein isolate powder.

[0039] Step 5: Mix glycerol epoxy resin and liquid paraffin at a weight ratio of 10:15 and heat to obtain a gel base. Mix soy protein isolate powder and theophylline at a weight ratio of 5:1 into the gel base and heat to 80°C for 50 minutes to obtain polyethylene glycol modified soy protein isolate gel.

[0040] Example 3

[0041] Step 1: Soak fresh soybeans in water for 1.3 hours and then freeze-dry them to obtain freeze-dried soybeans. Crush the freeze-dried soybeans to obtain crushed soybeans.

[0042] Step 2: Soak crushed soybeans in glycine buffer solution to dissolve soy protein isolate in the buffer solution. Filter to obtain soy protein isolate-buffer solution mixture. Concentrate the extracted soy protein isolate-buffer solution mixture to obtain soy protein isolate.

[0043] Step 3: Mix soy protein isolate and polyethylene glycol, and react at 55°C for 20-30 minutes to obtain modified soy protein isolate.

[0044] Step 4: Mix modified soy protein isolate, basalt powder and corn stalk powder in a weight ratio of 5-8:1:2.5, then granulate and crush to obtain soy protein isolate powder.

[0045] Step 5: Mix glycerol epoxy resin and liquid paraffin at a weight ratio of 12:17 and heat to obtain a gel base. Mix soy protein isolate powder and theophylline at a weight ratio of 15:3 into the gel base and heat to 90°C for 55 minutes to obtain polyethylene glycol modified soy protein isolate gel.

[0046] Example 4

[0047] Step 1: Soak fresh soybeans in water for 1.4 hours and then freeze-dry them to obtain freeze-dried soybeans. Crush the freeze-dried soybeans to obtain crushed soybeans.

[0048] Step 2: Soak crushed soybeans in glycine buffer solution to dissolve soy protein isolate in the buffer solution. Filter to obtain soy protein isolate-buffer solution mixture. Concentrate the extracted soy protein isolate-buffer solution mixture to obtain soy protein isolate.

[0049] Step 3: Mix soy protein isolate and polyethylene glycol and react at 60°C for 28 minutes to obtain modified soy protein isolate.

[0050] Step 4: Mix the modified soy protein isolate, basalt powder and corn stalk powder in a weight ratio of 7:1:2, then granulate and crush to obtain soy protein isolate powder.

[0051] Step 5: Mix glycerol epoxy resin and liquid paraffin at a weight ratio of 10:20 and heat to obtain a gel base. Mix soy protein isolate powder and theophylline at a weight ratio of 20:1 into the gel base and heat to 80°C for 55 minutes to obtain polyethylene glycol modified soy protein isolate gel.

[0052] Example 5

[0053] Step 1: Soak fresh soybeans in water for 1.5 hours and then freeze-dry them to obtain freeze-dried soybeans. Crush the freeze-dried soybeans to obtain crushed soybeans.

[0054] Step 2: Soak crushed soybeans in glycine buffer solution to dissolve soy protein isolate in the buffer solution. Filter to obtain soy protein isolate-buffer solution mixture. Concentrate the extracted soy protein isolate-buffer solution mixture to obtain soy protein isolate.

[0055] Step 3: Mix soy protein isolate and polyethylene glycol, and react at 55°C for 20-30 minutes to obtain modified soy protein isolate.

[0056] Step 4: Mix the modified soy protein isolate, basalt powder and corn stalk powder in a weight ratio of 5:1:3, then granulate and crush to obtain soy protein isolate powder.

[0057] Step 5: Mix glycerol epoxy resin and liquid paraffin at a weight ratio of 11:19 and heat to obtain a gel base. Mix soy protein isolate powder and theophylline at a weight ratio of 11:2 into the gel base and heat to 90°C for 50 minutes to obtain polyethylene glycol modified soy protein isolate gel.

[0058] Test example

[0059] Tests on the adsorption of heavy metal ions in water by polyethylene glycol modified soy protein isolate gels prepared in Examples 1-5.

[0060] Take 1L of simulated heavy metal wastewater, with mercury, chromium, cadmium and lead each containing 15mg / L, add polyethylene glycol modified soy protein isolate gel from Examples 1-5 of this invention, stir and let stand for 10 minutes, take the supernatant, and use an atomic absorption spectrophotometer to detect the heavy metal content. The specific results are shown in Table 1 below, where the concentration of each substance is in mg / L.

[0061] Table 1

[0062]

[0063]

[0064] As shown in Table 1, the polyethylene glycol-modified soy protein isolate gel prepared in this invention can effectively adsorb heavy metal ions. In other words, the polyethylene glycol-modified soy protein isolate gel prepared in this invention can be used to treat heavy metal pollutants in wastewater, effectively adsorb and fix heavy metal ions from water, purify water quality, and reduce the risk of heavy metal wastewater to the ecosystem and human health.

[0065] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing polyethylene glycol-modified soy protein isolate gel, characterized in that, include: Step 1: Crush the soybeans and collect the crushed soybeans; Step 2: Extract protein from the crushed soybeans to obtain soy protein isolate; Step 3: Modify the soy protein isolate using polyethylene glycol to obtain modified soy protein isolate; Step 4: Mix the modified soy protein isolate, basalt powder and corn stalk powder, then granulate and crush to obtain soy protein isolate powder; Step 5: Prepare polyethylene glycol-modified soy protein isolate gel by combining the soy protein isolate powder, theophylline, resin and liquid paraffin; In step 3, the soy protein isolate is modified with polyethylene glycol to obtain modified soy protein isolate. Specifically, the soy protein isolate and polyethylene glycol are mixed and reacted at 50-60°C for 20-30 minutes to obtain the modified soy protein isolate. In step 5, the soy protein isolate powder, theophylline, resin, and liquid paraffin are used to prepare a polyethylene glycol-modified soy protein isolate gel. Specifically, the resin and liquid paraffin are mixed and heated to obtain a gel substrate. The soy protein isolate powder and theophylline are then mixed into the gel substrate to obtain the polyethylene glycol-modified soy protein isolate gel. The weight ratio of the soy protein isolate powder, theophylline, resin, and liquid paraffin is 5-20:1-5:10-15:15-20. The resin is a glycerol epoxy resin.

2. The method for preparing polyethylene glycol-modified soy protein isolate gel according to claim 1, characterized in that, In step 1, the soybeans are crushed and the crushed soybeans are collected. Specifically, fresh soybeans are soaked in water for 1-1.5 hours and then freeze-dried to obtain freeze-dried soybeans. The freeze-dried soybeans are then crushed to obtain the crushed soybeans.

3. The method for preparing polyethylene glycol-modified soy protein isolate gel according to claim 1, characterized in that, In step 2, the crushed soybeans are subjected to protein extraction to obtain soy protein isolate. Specifically, the crushed soybeans are soaked in a buffer solution to dissolve the soy protein isolate in the buffer solution, filtered to obtain a soy protein isolate-buffer solution mixture, and the extracted soy protein isolate-buffer solution mixture is concentrated to obtain the soy protein isolate.

4. The method for preparing polyethylene glycol-modified soy protein isolate gel according to claim 1, characterized in that, In step 4, the corn stalk powder is prepared by soaking corn stalks in an alkaline solution, filtering after soaking, and then subjecting them to high-temperature treatment. The corn stalks after high-temperature treatment are then crushed to obtain corn stalk powder.

5. The method for preparing polyethylene glycol-modified soy protein isolate gel according to claim 1, characterized in that, The soy protein isolate powder and theophylline are mixed into the gel substrate and then heated to 80-100℃ and held for 50-60 minutes to obtain the polyethylene glycol modified soy protein isolate gel.

6. A polyethylene glycol-modified soy protein isolate gel, characterized in that, The polyethylene glycol-modified soy protein isolate gel is prepared by the preparation method according to any one of claims 1-5.

7. The application of the polyethylene glycol-modified soy protein isolate gel according to claim 6, characterized in that, This includes the application of the polyethylene glycol-modified soy protein isolate gel in the adsorption of heavy metals; And / or, the application of the polyethylene glycol-modified soy protein isolate gel in the preparation of heavy metal adsorption products.

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