Natural plant composition with improved sleep function and application thereof

Abstract

The application discloses a natural plant composition with sleep improvement function and application thereof, which is composed of 20-70 parts by weight of pumpkin seed extract, 10-30 parts by weight of poria cocos extract, 10-30 parts by weight of balm mint extract and 10-30 parts by weight of German chamomile flower extract, wherein the extracts are obtained by using water and / or ethanol as an extraction solvent. The natural plant composition has simple raw material components, and can be directly mixed after extraction; after scientific and reasonable compounding, the natural plant composition promotes each other, significantly improves sleep by adjusting the change of human body hormones and neurotransmitters, has no toxic side effect and is high in safety.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of health food, and relates to a natural plant composition with sleep improvement function and application thereof. BACKGROUND

[0002] Insomnia is a kind of disease caused by heart and mind deficiency or restlessness, which leads to normal sleep, and is clinically manifested as insufficient sleep time, insufficient sleep depth, inability to eliminate fatigue, etc. In mild cases, it is difficult to fall asleep or sleep without rest, and in severe cases, it is impossible to sleep at night, so that people often feel tired, headache, dizziness, palpitation and forgetfulness after waking up, which seriously affects people's normal life and work.

[0003] Western medicine has the advantages of quick effect and clear effect in the treatment of insomnia. At present, the drugs for treating insomnia include benzodiazepines, non-benzodiazepines and antidepressants. Benzodiazepines can easily cause psychomotor impairment, memory impairment, drug addiction and rebound insomnia after drug withdrawal. Non-benzodiazepines have less side effects such as insomnia rebound, withdrawal syndrome and memory impairment, but are prone to drug resistance, addiction and dependence.

[0004] At present, the health food for improving sleep on the market is mainly composed of synthetic melatonin, which can improve sleep quality, but the substance can cause excessive drowsiness, affect daytime alertness and attention, and have side effects such as dizziness, headache and muscle soreness. Some fragrance candles and sleep spray products claim to promote sleep, but in fact, sleep is a state of inhibition and calmness of the brain and body, and the stimulation of fragrance, drugs and other odors is contrary to the situation, which only makes the brain more excited.

[0005] Therefore, it is of great significance to develop a product which can be used for a long time, has few side effects and has significant sleep improvement effect. SUMMARY

[0006] In view of the problems existing in the existing sleep improvement drugs or food, the purpose of the present application is to provide a natural plant composition with sleep improvement function, which has simple raw material components, can be directly mixed after extraction, and can promote each other after scientific and reasonable compounding, significantly improve sleep by regulating the changes of human body hormones and neurotransmitters, has no toxic side effects and high safety.

[0007] In order to achieve the above technical purpose, the technical scheme adopted by the present application is as follows:

[0008] A natural plant composition with sleep improvement function, which is composed of the following components in parts by weight:

[0009]

[0010] The extracts are all obtained by using water and / or ethanol as extraction solvents.

[0011] Preferably, the natural plant composition is composed of the following components in parts by weight:

[0012]

[0013] In the present invention, pumpkin seed extract supplements natural GABA to the human body, lemon balm extract can increase the release of GABA in brain tissue, thereby improving the GABA concentration in the brain, supplementing exogenous GABA while increasing the production of endogenous GABA, Poria cocos extract can increase the content of GABA receptors in the body, and German chamomile flower extract can enhance the activity of GABA receptors. The components promote each other and synergistically improve sleep.

[0014] The natural plant composition of the present invention has been confirmed by animal experimental results. After oral administration to mice for 30 days, experiments such as experiments on prolonging the sleep time of sodium pentobarbital and the subthreshold hypnotic dose of sodium pentobarbital were conducted. The experimental results were all positive and there was no obvious direct sleep effect. This further confirms that the natural plant composition of the present invention has the effect of significantly improving sleep, and the effect is slightly better than the commonly used dose of GABA.

[0015] The present invention also provides an application of the natural plant composition having the function of improving sleep, which is used to prepare functional food or health food having the function of improving sleep.

[0016] Preferably, auxiliary materials are added to the natural plant composition to prepare tablets, oral liquids, capsules, granules, powders or pills to obtain functional foods or health foods that improve sleep function.

[0017] The advantages of the present invention are:

[0018] The present invention is a natural plant composition with simple raw material components, no side effects on the human body, high product safety, and can be directly mixed after extraction, which is simple to operate. According to the requirements of the "Sleep Improvement Function Test Method" in the national "Technical Specifications for Inspection and Evaluation of Health Food", an experiment was designed to evaluate the sleep improvement activity function of the natural plant composition of the present invention. The results showed that the natural plant composition of the present invention promotes each other after scientific and reasonable combination, and has a significant improvement effect on sleep disorders by regulating changes in the body's own hormones and neurotransmitters. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is the result of the experiment on prolonging sleep time with sodium pentobarbital;

[0020] Figure 2 This is the result of the sodium barbital sleep latency experiment;

[0021] Note: ## indicates that the difference is extremely significant compared with the blank control group, with P value < 0.01; # indicates that the difference is significant compared with the blank control group, with 0.01 < P value < 0.05. DETAILED DESCRIPTION

[0022] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be described below in conjunction with the embodiments of the present invention and the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0023] In the present invention, all extracts are extracts obtained by conventional extraction processes using water and / or ethanol as extraction solvents, for example:

[0024] Pumpkin seed extract was prepared as follows:

[0025] After shelling the pumpkin seeds, crush them, soak them in 10 times the amount of distilled water for 2 hours, and decoct them twice, adding 10 times the amount of water for 1.5 hours in the first decoction and 8 times the amount of water for 1.5 hours in the second decoction. The decoctions are combined, filtered, and the filtrate is mixed and concentrated using a rotary evaporator. The pumpkin seed extract is dried and crushed in a vacuum drying oven to obtain the pumpkin seed extract.

[0026] Melissa officinalis extract was prepared as follows:

[0027] The lemon balm raw material is added into a grinder and crushed, the crushed raw material is weighed, 10 times the weight volume of water is added, hot reflux extraction is performed for 2 hours, the extract is filtered, the residue is added with 8 times the weight volume of water, hot reflux extraction is performed for 1 hour, the extract is filtered, the combined filtrate is concentrated, and vacuum drying is performed to obtain the lemon balm extract.

[0028] Poria cocos extract was prepared as follows:

[0029] The Poria cocos raw material was added into a grinder and crushed, sieved after crushing, and ultrasonically extracted for 30 minutes by adding 10 times the volume of water. The extract was filtered, concentrated, and vacuum-dried to obtain the Poria cocos extract.

[0030] German chamomile flower extract was prepared as follows:

[0031] German chamomile flower petals were added with 25 times water and heated to boiling, extracted for 1 hour and filtered, the filtrate was concentrated by rotary evaporator, dried in a vacuum drying oven and then crushed to obtain concentrated and dried German chamomile flower extract.

[0032] 1. The raw material components of Example 1 and Comparative Examples 1-9 of the present invention are shown in Table 1:

[0033] Table 1 Composition of the composition with sleep-improving function in parts by weight

[0034]

[0035] 2. Animal trials to evaluate sleep-improving efficacy:

[0036] 330 SPF-grade healthy male mice aged 6-8 weeks and weighing 18-22 g were housed at (24±2)°C and a relative humidity of 39%-69%. They were divided into 11 groups (30 mice per group, with experiments 2.2, 2.3, and 2.4 performed on 10 mice per group): a blank control group (distilled water), a positive control group (GABA), Example 1 group, and Comparative Examples 1-8 groups. The GABA dose was 50 mg / kg, and the dose in all other groups was 200 mg / kg.

[0037] 2.1 Direct Sleep Experiment: Eleven groups of mice were administered varying doses of the test sample at 9:00 AM daily, with a gavage volume of 0.2 mL / 20 g, for 30 consecutive days. After the final gavage, the mice in each group were observed for sleep. Sleep was assessed by the loss of the righting reflex (normal mice will immediately right themselves when in dorsal recumbency). If a mouse failed to right itself for more than 60 seconds, it was considered to have lost the righting reflex and entered sleep; recovery of the righting reflex indicated awakening. The results are shown in Table 1.

[0038] Table 1 Results of direct sleep experiment

[0039]

[0040]

[0041] As shown in Table 1, no sleep phenomenon occurred in the experimental mice at the doses administered orally in each experimental group, indicating that the experimental groups had no direct sleep effect.

[0042] 2.2 Experiment on prolonging the sleep time of sodium pentobarbital: 11 groups of mice were given different doses of the test sample at 9:00 a.m. daily, with an oral gavage volume of 0.2 mL / 20 g, for 30 consecutive days. 30 minutes after the last oral gavage, each group of animals was intraperitoneally injected with 45 mg / kg BW sodium pentobarbital, with an injection volume of 0.2 mL / 20 g. The loss of righting reflex was used as an indicator to observe whether the test sample could prolong the sleep time of sodium pentobarbital. The results are as follows Figure 1 shown.

[0043] Figure 1 As can be seen, after 30 days of continuous gavage, both the examples and the comparative examples were able to prolong the sleep time of the mice compared with the blank control group. Among them, the effects of Example 1 and the positive control group were significant, and Example 1 was slightly higher than the positive control group, but better than comparative examples 1-8.

[0044] 2.3 Subthreshold Dosage of Sodium Pentobarbital for Hypnosis: Eleven groups of mice received varying doses of the test sample daily at 9:00 AM for 30 consecutive days, with a gavage volume of 0.2 mL / 20 g. Thirty minutes after the last gavage, each group received an intraperitoneal injection of 25 mg / kg BW sodium pentobarbital (0.2 mL / 20 g). The criterion for sleep onset was loss of righting reflex for at least 60 seconds. The number of animals that fell asleep within 30 minutes was recorded. The sleep incidence rate was calculated as the number of sleeping animals divided by the total number of animals. The results are shown in Table 2.

[0045] Table 2 Results of subthreshold dose hypnosis experiments with sodium pentobarbital

[0046]

[0047]

[0048] As shown in Table 2, the incidence of sleep in each test group was improved. In addition, Example 1 and the positive control group showed significant differences compared with Comparative Examples 1-8, and the incidence of mice falling asleep within 30 minutes was significantly increased, indicating that the effect of the composition of the present invention was very significant.

[0049] 2.4 Sodium barbital sleep latency experiment: 11 groups of mice were given different doses of the test sample at 9:00 a.m. daily, with an oral gavage volume of 0.2 mL / 20 g, for 30 consecutive days. 30 minutes after the last oral gavage, each group of animals was intraperitoneally injected with 260 mg / kg BW sodium barbital, with an injection volume of 0.2 mL / 20 g. The effect of the test sample on the sodium barbital sleep latency was observed using the loss of antidromic reflex as an indicator. The results are shown in the figure below. Figure 2 shown.

[0050] like Figure 2 As shown, after continuous gavage for 30 days, the sleep latency of mice in each test group was shortened compared with the blank control group, among which Example 1, the positive control group and comparative examples 1-3 had more significant effects, and Example 1 was even better than the positive control group.

[0051] In summary, both Example 1 and the positive control group showed positive results, and the sleep-improving compositions in each group had no obvious direct sleep effect, indicating that the composition of the present invention has a significant effect of synergistically improving sleep.

Claims

1. A natural plant composition with sleep-improving function, characterized in that: By weight, it is composed of the following components: 40 parts by weight of pumpkin seed extract; 20 parts by weight of Poria cocos extract; 20 parts by weight of lemon balm extract; 20 parts by weight of German chamomile flower extract; Pumpkin seed extract was prepared as follows: After shelling the pumpkin seeds, crush them, soak them in 10 times the amount of distilled water for 2 hours, and decoct them twice, adding 10 times the amount of water for 1.5 hours in the first decoction and 8 times the amount of water for 1.5 hours in the second decoction. The decoctions are combined, filtered, and the filtrate is mixed and concentrated using a rotary evaporator. The pumpkin seed extract is dried and crushed in a vacuum drying oven to obtain the pumpkin seed extract. Melissa officinalis extract was prepared as follows: The lemon balm raw material is added into a grinder and crushed, the crushed raw material is weighed, 10 times the weight volume of water is added, and the hot reflux extraction is performed for 2 hours, the extract is filtered, the residue is added into 8 times the weight volume of water, and the hot reflux extraction is performed for 1 hour, the extract is filtered, the combined filtrate is concentrated, and vacuum dried to obtain the lemon balm extract; Poria cocos extract was prepared as follows: The raw material of Poria cocos was added into a grinder and crushed, sieved after crushing, and ultrasonically extracted for 30 minutes by adding 10 times the volume of water. The extract was filtered, concentrated, and vacuum-dried to obtain the Poria cocos extract; German chamomile flower extract was prepared as follows: German chamomile flower petals were added with 25 times water and heated to boiling, extracted for 1 hour, filtered, and the filtrate was concentrated by rotary evaporator, dried in a vacuum drying oven, and then crushed to obtain the concentrated and dried German chamomile flower extract.

2. The use of the natural plant composition according to claim 1, characterized in that: The invention can be used for preparing functional food or health food for improving sleep function.

3. The use according to claim 2, characterized in that Auxiliary materials are added to the natural plant composition to prepare tablets, oral liquids, capsules, granules, powders or pills to obtain functional food or health food for improving sleep function.

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