A crop hydroponic test root culture observation device and its use method

Abstract

The present invention relates to the technical field of plant physiological testing devices and discloses a device for observing the cultivation of hydroponic crop root systems and a method for using the device. The device comprises a cultivation cup containing a cultivation solution, a cultivation rack movably mounted on the top of the cup, cultivation openings uniformly formed on the bottom and surrounding surfaces of the cultivation rack, and a test crop planted through the cultivation openings. The test crop plant comprises a root system immersed in the cultivation solution, and an observation rail fixedly connected to one side of the cultivation cup. The observation rail extends from one side of the cultivation cup to the bottom of the cultivation cup. The present invention replaces the traditional cylindrical opaque pot with a transparent rectangular pot, eliminating the need to wait for the root system of the test crop to grow to a specific growth period before removing the root system of the test crop for relevant measurement. Furthermore, the present invention avoids observation errors caused by directly observing the magnified image through a cylindrical glass tube, thereby making the data obtained by the device more accurate during the observation process.

Description

Technical Field

[0001] The present invention relates to the technical field of plant physiological test devices, in particular to a crop hydroponic test root culture observation device and a use method thereof. Background Art

[0002] The root system is an important organ for plant absorption, drainage, synthesis, storage, secretion, regulation, fixation and support, and plays an important role in plant growth and development. The root system is particularly important for plants. It is the basis and center of the overall development of the tree. Therefore, studying the morphological characteristics, physiological functions, growth and development laws, root ecology, and root regulation technology of the root system has important theoretical and practical significance. However, the naturally grown root system is deep underground. Due to the complexity of soil environmental conditions and the opacity of soil media, it is difficult to observe and measure it in situ. To date, there has been an in situ root observation system, but the device can only observe the roots around the root canal and cannot observe the entire root morphology and root structure. Therefore, hydroponic experiments have become an important means of root observation.

[0003] Most common root system studies involve hydroponically growing crops in potting barrels, potting boxes, or root tubes (cylindrical PVC tubes). Once the plants reach a specific growth period, the roots are removed and cleaned for measurements of morphology, configuration, and physiological indicators. This method of root system research is cumbersome and time-consuming to sample, and improper operation can damage the roots, affecting the accuracy of the experiment. Alternatively, crops are planted in transparent cylindrical glass tubes. Although observation is possible, it is difficult to directly obtain accurate root data due to the free growth of the roots in the culture solution. Furthermore, it is difficult to obtain physiological changes within the roots, such as root activity and ion absorption dynamics. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a root cultivation observation device for crop hydroponic experiments and a method of using the same.

[0005] In order to achieve the above-mentioned purpose of the invention, the technical solution adopted by the present invention is as follows: a crop hydroponic test root culture observation device, comprising a cultivation cup, a cultivation liquid is placed inside the cultivation cup, a cultivation rack is movably placed on the top of the cultivation cup, the bottom surface and the four sides of the cultivation rack are evenly provided with cultivation openings, and experimental crops are cultured through the cultivation openings, the experimental crops include crop roots immersed in the cultivation liquid, an observation rail is fixedly connected to one side of the interior of the cultivation cup, the observation rail extends from one side of the cultivation cup to the bottom of the cultivation cup, an observation box is movably connected to the observation rail, a water pump is fixedly installed inside the observation box, the water pump includes a water outlet and a water inlet, a motor is fixedly installed on one side of the water pump, one end of the motor is provided with a gear, and the observation box is fixedly installed with a water pump. The shift rail is provided with a rail groove that matches the motor gearbox. The other side of the water pump is connected to a detection cavity through a water inlet. A detection port is opened on the outside of the detection cavity. The inner side of the detection cavity is fixedly connected to a probe spring, and is fixedly connected to a probe integrated board through the probe spring. Probes are fixedly installed on one side of the probe integrated board, and the probes are evenly distributed. The bottom end of the probe integrated board is electrically connected to an integrated controller and a power supply group through a data line. The integrated controller and the power supply group centrally control the motor, water pump and probe integrated board. Clamp rod shafts are fixedly installed on both sides of the detection port on one side of the observation box, and are movably connected to a clamp rod through the clamp rod shaft. The clamp rod is "L"-shaped, and a rotating shaft is fixedly installed at the end of the "L"-shaped short end of the clamp rod, and is movably connected to the edge of the probe integrated board through the rotating shaft.

[0006] Preferably, the cultivation cup is in the shape of a transparent cuboid.

[0007] Preferably, the observation box can slide up and down along the observation rail. When the observation box slides to the bottom position of the observation rail, the front of the observation box faces upward and is located at the center of the bottom of the cultivation cup. The motor controls the observation box to stay at any height of the observation rail.

[0008] Preferably, when the observation box is located at the bottom of the cultivation cup, the water inlet opens upward, and the opening direction of the water outlet is perpendicular to the opening direction of the water inlet.

[0009] Preferably, the long end of the "L"-shaped clamping rod is provided with an arc-shaped friction plate.

[0010] The present invention has the following beneficial effects:

[0011] 1. The present invention replaces the traditional cylindrical opaque pot with a transparent rectangular shape. This eliminates the need to wait until the roots of the test crops have grown to a specific growth period before removing the roots for relevant measurements. It also avoids the observation error caused by directly observing the magnified image through a cylindrical glass tube, making the data obtained by the device during the observation process more accurate.

[0012] 2. The present invention uses an observation box installed at the bottom of the observation rail to form a top-down water flow at the center of the cultivation cup, thereby gathering the crop roots scattered in the cultivation cup toward the center and straightening them downward. Combined with the transparent cultivation cup, the accurate measurement of the growth length of the crop roots is ensured, thereby obtaining the accurate length of the crop roots and avoiding the problem of inaccurate length data measurement caused by the crop roots growing freely in the cultivation solution.

[0013] 3. The present invention sets a water pump in the observation box, so that the culture liquid in the culture cup is sucked by the water pump to flow from the water inlet and then out of the water outlet, driving the culture liquid to flow, thereby avoiding the problem of uneven concentration of the culture liquid caused by precipitation of the culture liquid in the culture cup after being left still for a long time.

[0014] 4. The present invention controls the observation box to stay at any height of the observation track through an integrated controller and a power supply group, thereby facilitating the measurement of data at any position of the crop root system. The water pump is activated to adsorb the crop root system, so that the crop root system contacts the probe and the probe at the contact point penetrates into the crop root system, thereby monitoring the physiological change characteristics inside the crop root system, such as root activity, ion absorption dynamics, etc., thereby realizing the collection of internal data of the crop root system.

[0015] 5. The present invention uses multiple groups of evenly distributed probes and utilizes the contact between the probes at different positions and the crop roots to measure the width of the crop roots at that height, that is, the diameter of the crop roots at that point. Combined with the measured length of the crop roots at that time, more accurate external data of the crop roots at that time can be easily obtained, making the measurement results of the detection device more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a side sectional view of the overall structure of the present invention;

[0017] Figure 2 This is a side cross-sectional view of the root length measurement state of the present invention;

[0018] Figure 3 Schematic diagram of the internal structure of the observation box of the present invention;

[0019] Figure 4 For the present invention Figure 3 A partial enlarged schematic diagram of the structure at center A;

[0020] Figure 5 It is a bottom-view cross-sectional view of the overall structure of the present invention;

[0021] Figure 6 For the present invention Figure 5 A schematic diagram of the partial enlargement of the structure at point B in the middle.

[0022] In the figure: 1. Cultivation cup; 101. Cultivation solution; 2. Cultivation rack; 201. Cultivation port; 3. Test crop; 301. Crop root system; 4. Observation track; 5. Observation box; 6. Probe integrated board; 601. Probe; 602. Probe spring; 7. Water pump; 701. Water outlet; 702. Water inlet; 8. Motor; 9. Clamping rod; 901. Clamping rod shaft; 10. Integrated controller and power pack. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] See also Figure 1-2 A device for observing the root system of a hydroponic test crop comprises a cultivation cup 1, which is a transparent rectangular parallelepiped. A cultivation solution 101 is placed inside the cultivation cup 1. A cultivation rack 2 is movably placed on the top of the cultivation cup 1. Cultivation openings 201 are evenly opened on the bottom and around the cultivation rack 2. A test crop 3 is cultured through the cultivation openings 201. The test crop 3 includes a crop root 301 immersed in the cultivation solution 101. An observation movable is fixedly connected to one side of the interior of the cultivation cup 1. Rail 4, the observation rail 4 extends from one side of the cultivation cup 1 to the bottom of the cultivation cup 1, and an observation box 5 is movably connected to the observation rail 4. The observation box 5 can slide up and down along the observation rail 4. When the observation box 5 slides to the bottom position of the observation rail 4, the front of the observation box 5 faces upward and is located at the center of the bottom surface of the cultivation cup 1. A water pump 7 is fixedly installed inside the observation box 5. The water pump 7 includes a water outlet 701 and a water inlet 702. When the observation box 5 is located at the bottom surface of the cultivation cup 1, the water inlet 702 is open Upward, the opening direction of the water outlet 701 is perpendicular to the opening direction of the water inlet 702. When the observation box 5 moves along the observation rail 4, the culture liquid 101 in the cultivation cup 1 is attracted by the water pump 7 to flow from the water inlet 702 and then out of the water outlet 701, driving the culture liquid 101 to flow, avoiding the problem of uneven concentration of the culture liquid 101 caused by precipitation of the culture liquid in the cultivation cup 1 after being left still for a long time. When it is necessary to collect the growth length data of the crop root 301, the observation box 5 is set The device is stationary at the center of the bottom of the cultivation cup 1, and the water pump 7 draws the cultivation liquid 101 inward from the water inlet 702, thereby forming a top-down water flow at the center of the cultivation cup 1, so that the crop roots 301 scattered in the cultivation cup 1 gather toward the center and straighten downward. Since the cultivation cup 1 is a transparent rectangular parallelepiped, it is convenient to use the device to directly obtain the original root length data, avoiding the problem of errors caused by the cylindrical glass tube amplifying the image of the crop roots 301, and facilitating the observation of root growth.

[0025] See also Figure 3-4 , a motor 8 is fixedly installed on one side of the water pump 7, and a gear is provided at one end of the motor 8. A rail groove matching the gear box of the motor 8 is provided on the observation rail 4. The motor 8 can freely drive the observation box 5 to move up and down on the observation rail 4, and control the observation box 5 to stay at any height of the observation rail 4. The other side of the water pump 7 is connected to the detection chamber through the water inlet 702, and a detection port is provided on the outside of the detection chamber. A probe spring 602 is fixedly connected to the inside of the detection chamber, and a probe integrated board 6 is fixedly connected to the probe spring 602. A probe 601 is fixedly installed on one side of the probe integrated board 6, and the probes 601 are evenly distributed. The bottom end of the probe integrated board 6 is electrically connected to the integrated controller and the power supply group 10 through a data line. The integrated controller and the power supply group 10 distribute and control the motor 8, the water pump 7 and the probe integrated board 6, thereby controlling the staying position of the observation box 5, the size of the water flow disturbed by the water pump 7 and the detection port at the probe 601. After the observation box 5 stays still at the set height, the water pump 7 is started to absorb the crop root system 301, so that the crop root system 301 contacts the probe 601 and the probe 601 at the contact point penetrates into the crop root system 301, and the physiological change characteristics inside the crop root system 301, such as root activity, ion absorption dynamics, etc., are monitored, thereby realizing the collection of internal data of the crop root system 301. At the same time, according to the different contact positions of the evenly distributed probes 601 and the crop root system 301, the data on the probe 601 is transmitted to the probe integrated board 6, and then transmitted to the integrated controller and power supply group 10 through the data line. The width of the crop root system 301 at the height, that is, the diameter of the root system at that location, can be measured through the data collected by the probes 601 at different positions. Combined with the measured length of the crop root system 301 at this time, more accurate external data of the crop root system 301 at this time can be easily obtained, making the measurement result of the detection device more accurate.

[0026] See also Figure 4-5 , clamping rod shafts 901 are fixedly installed on both sides of the detection port on one side of the observation box 5, and a clamping rod 9 is movably connected to the clamping rod shaft 901. The clamping rod 9 is "L"-shaped, and the long end of the "L"-shaped clamping rod 9 is provided with an arc-shaped friction plate, so that it can grasp the crop root 301 more firmly. A rotating shaft is fixedly installed at the end of the "L"-shaped short end of the clamping rod 9 and is movably connected to the edge of the probe integrated board 6 through the rotating shaft. After the water pump 7 is turned on to adsorb the crop roots 301 close to the probe integrated board 6, the crop roots 301 contact and press the probe 601, so that the probe integrated board 6 moves toward the inside of the adsorber and presses the probe spring 602. At this time, the probe integrated board 6 pulls the "L"-shaped short end of the clamping rod 9 to rotate the clamping rod 9 around the clamping rod shaft 901, thereby pulling the long end of the clamping rod 9 to rotate inward, pressing the animal root 301 inward and close to the probe 601, thereby ensuring the effect of the probe 601 penetrating the crop root 301, and improving the accuracy of monitoring the internal data of the root system and its external diameter data.

[0027] The working principle of the method of use of the present invention is as follows:

[0028] When in use, the cultivation liquid 101 is injected into the cultivation cup 1 of the observation device, the cultivation rack 2 is placed on the top of the cultivation cup 1, and the test crop 3 to be observed is placed on the cultivation rack 2. The crop root system 301 at the bottom extends to the bottom of the cultivation cup 1 through the cultivation port 201. The transparent rectangular cultivation cup 1 is convenient for regular observation of the accurate state of the crop root system 301, and will not produce optical deformation due to the curved side wall of the cultivation cup 1. During regular observation, the motor 8 is first controlled by the integrated controller and the power supply group 10. The wheel at one end of the motor 8 rotates and moves along the rail groove on the observation rail 4, driving the observation box 5 to move along the observation rail 4 to the bottom of the cultivation cup 1. Then the integrated controller and the power supply group 10 control the water pump 7 to start, and the water pump 7 sucks the cultivation liquid through the water inlet 702. The water pump 7 pumps the cultivation liquid 101 and sprays it out from the water outlet 701. This process forms a top-down water flow at the center of the cultivation cup 1, so that the crop roots 301 scattered in the cultivation cup 1 gather toward the center and straighten downward. The transparent cultivation cup 1 ensures the accurate measurement of the growth length of the crop roots 301, thereby obtaining the accurate length of the crop roots 301, avoiding the problem of inaccurate length data measurement caused by the crop roots 301 growing freely in the cultivation liquid 101; at the same time, the disturbance of the cultivation liquid 101 by the water pump 7 mixes the cultivation liquid 101 deposited at the bottom of the cultivation cup 1 evenly, causing the cultivation liquid 101 to flow, avoiding the problem of uneven concentration of the cultivation liquid 101 caused by the precipitation of the cultivation liquid in the cultivation cup 1 after a long period of standing. Title: After the measurement of the length of the crop root system 301 is completed, the integrated controller and the power supply group 10 control the water pump 7 to turn off, and then control the motor 8 to turn on, driving the observation box 5 to move up along the observation rail 4 as a whole. When it rises to the set height, it stops, and the water pump 7 is turned on again. The crop root system 301 at this height position is attracted by the water inlet 702, so that it is close to the probe 601 and pushes the probe integrated board 6 inward, so that the probe integrated board 6 moves toward the inside of the adsorber and presses the probe spring 602. At this time, the probe integrated board 6 pulls the "L"-shaped short end of the clamping rod 9 to rotate the clamping rod 9 around the clamping rod axis 901, thereby pulling the long end of the clamping rod 9 to rotate inward, pressing the animal root system 301 inward to close to the probe 601, thereby ensuring that the probe 601 penetrates the action. The probe 601 monitors the physiological change characteristics inside the crop root system 301, such as root activity, ion absorption dynamics, etc., so as to realize the collection of internal data of the crop root system 301. At the same time, according to the different contact positions between the evenly distributed probes 601 and the crop root system 301, the data on the probe 601 is transmitted to the probe integrated board 6, and then transmitted to the integrated controller and power supply group 10 through the data line. The width of the crop root system 301 at this height, that is, the diameter of the root system at this height, can be inferred through the data collected by the probes 601 at different positions. Combined with the measured length of the crop root system 301 at this time, more accurate external data of the crop root system 301 at this time can be easily obtained, making the measurement result of the detection device more accurate.

[0029] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0030] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A crop hydroponic test root culture observation device, comprising a cultivation cup (1), wherein a cultivation solution (101) is placed inside the cultivation cup (1), a cultivation rack (2) is movably placed on the top of the cultivation cup (1), and cultivation openings (201) are evenly opened on the bottom surface and the surrounding of the cultivation rack (2), and a test crop (3) is cultured through the cultivation opening (201), wherein the test crop (3) includes a crop root system (301) immersed in the cultivation solution (101); characterized in that: An observation rail (4) is fixedly connected to one side of the cultivation cup (1), and the observation rail (4) extends from one side of the cultivation cup (1) to the bottom of the cultivation cup (1); an observation box (5) is movably connected to the observation rail (4), and a water pump (7) is fixedly installed inside the observation box (5); the water pump (7) includes a water outlet (701) and a water inlet (702); a motor (8) is fixedly installed on one side of the water pump (7), and a gear is provided at one end of the motor (8); a rail groove matching the gear box of the motor (8) is provided on the observation rail (4); the other side of the water pump (7) is connected to a detection chamber through the water inlet (702), and a detection port is provided on the outside of the detection chamber, and a probe spring (602) is fixedly connected to the inside of the detection chamber, and a probe spring (602) is fixedly installed on the inside of the detection chamber, and a probe spring (602) is fixedly installed on the inside of the detection chamber. The probe spring (602) is fixedly connected to the probe integrated board (6); a probe (601) is fixedly installed on one side of the probe integrated board (6), and the probes (601) are evenly distributed; the bottom end of the probe integrated board (6) is electrically connected to the integrated controller and power supply group (10) through a data line, and the integrated controller and power supply group (10) centrally control the motor (8), the water pump (7) and the probe integrated board (6); a clamping rod shaft (901) is fixedly installed on both sides of the detection port on one side of the observation box (5), and is movably connected to a clamping rod (9) through the clamping rod shaft (901), the clamping rod (9) is "L"-shaped, and a rotating shaft is fixedly installed at the end of the "L"-shaped short end of the clamping rod (9), and is movably connected to the edge of the probe integrated board (6) through the rotating shaft.

2. The root cultivation observation device for crop hydroponic experiment according to claim 1, characterized in that: The cultivation cup (1) is in the shape of a transparent cuboid.

3. The root cultivation observation device for crop hydroponic experiment according to claim 1, characterized in that: The observation box (5) can slide up and down along the observation rail (4). When the observation box (5) slides to the bottom position of the observation rail (4), the front of the observation box (5) faces upward and is located at the center of the bottom surface of the cultivation cup (1). The motor (8) controls the observation box (5) to stay at any height of the observation rail (4).

4. The root cultivation observation device for crop hydroponic experiment according to claim 1, characterized in that: When the observation box (5) is located at the bottom of the cultivation cup (1), the water inlet (702) opens upward, and the opening direction of the water outlet (701) is perpendicular to the opening direction of the water inlet (702).

5. The root cultivation observation device for crop hydroponic experiment according to claim 1, characterized in that: The long end of the "L"-shaped clamping rod (9) is provided with an arc-shaped friction plate.

6. A method for using the crop hydroponic test root culture observation device according to any one of claims 1 to 5, characterized in that: A cultivation liquid (101) is injected into the cultivation cup (1) of the observation device, a cultivation rack (2) is placed on the top of the cultivation cup (1), and a test crop (3) to be observed is placed on the cultivation rack (2), and the crop root system (301) at the bottom of the test crop (3) to be observed extends to the bottom of the cultivation cup (1) through the cultivation port (201); The accurate state of the crop root system (301) is regularly observed through a transparent rectangular cultivation cup (1). During regular observation, the motor (8) is first controlled by the integrated controller and the power supply group (10). The gear at one end of the motor (8) rotates and moves along the upper rail groove of the observation rail (4), driving the observation box (5) to move along the observation rail (4) to the bottom end of the cultivation cup (1). Then, the integrated controller and the power supply group (10) control the water pump (7) to start, and the water pump (7) sucks the cultivation liquid (101) through the water inlet (702) and sprays it from the water outlet (701). This process forms a water flow from top to bottom at the center of the cultivation cup (1), so that the water dispersed in the cultivation cup (1) is dispersed. ) gathers toward the center and straightens downward, and the transparent cultivation cup (1) ensures accurate measurement of the growth length of the crop root system (301), thereby obtaining the accurate length of the crop root system (301), and avoiding the problem of inaccurate length data measurement caused by the crop root system (301) growing freely in the cultivation solution (101); at the same time, the water pump (7) disturbs the cultivation solution (101), mixes the cultivation solution (101) deposited at the bottom of the cultivation cup (1), and makes the cultivation solution (101) flow, thereby avoiding the problem of uneven concentration of the cultivation solution (101) caused by the precipitation of the cultivation solution in the cultivation cup (1) after being left still for a long time; After the measurement of the length of the crop root system (301) is completed, the integrated controller and power supply group (10) control the water pump (7) to turn off, and then control the motor (8) to turn on, driving the observation box (5) as a whole to move up along the observation rail (4), and stop when it rises to the set height. The water pump (7) is turned on again, and the crop root system (301) at the height position is attracted by the water inlet (702) so as to move closer to the observation box (5) until it is close to the probe (601) and pushes the probe integrated board (6) inward, so that the probe integrated board (6) moves into the interior of the adsorber to press the probe spring (602). At this time, the probe integrated board (6) pulls the "L"-shaped short end of the clamping rod (9) to rotate the clamping rod (9) around the clamping rod axis (901), thereby pulling the long end of the clamping rod (9) to rotate inward, pressing the plant root system (301) inward to close to the probe (601), thereby ensuring that the probe (601) penetrates. The probe (601) monitors the physiological change characteristics inside the crop root system (301), and the physiological change characteristics include root activity and ion absorption dynamics, thereby realizing the collection of internal data of the crop root system (301). At the same time, according to the different contact positions between the evenly distributed probes (601) and the crop root system (301), the data on the probe (601) is transmitted to the probe integrated board (6), and then transmitted to the integrated controller and power supply group (10) through the data line. The width of the crop root system (301) at the height, that is, the diameter of the root system at the height, can be calculated by the data collected by the probes (601) at different positions. Combined with the measured length of the crop root system (301) at this time, more accurate external data of the crop root system (301) at this time can be easily obtained, making the measurement results of the crop hydroponic test root culture observation device more accurate.

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