Composition and method for lily bulb breeding

Abstract

The present invention relates to the field of plant cultivation, and in particular to a composition and method for lily bulb propagation. The composition comprises a lily bulb propagation matrix and a lily bulb expander; the lily bulb propagation matrix comprises pine bark, perlite and vermiculite, and the mass ratio of pine bark, perlite and vermiculite in the matrix is ​​1-10:1-10:1-10; the lily bulb expander is a 5%-10% mass fraction of Trichoderma harzianum wettable powder solution. The composition of the present invention can increase the aboveground fresh weight, underground fresh weight and root weight of lily plants, increase the weight of lily bulbs, increase the circumference of lily bulbs, and facilitate the later bulb propagation.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation, and more specifically to a composition and method for propagating lily bulbs. Background Technology

[0002] The main raw materials for plant growth substrates are typically peat moss, perlite, coconut sugar, rock wool, and gravel. Peat moss is mainly distributed in Northern Europe and is a product of wetland environments. Importing it is expensive, and over-exploitation inevitably impacts wetlands and the ecological environment. Therefore, developing and utilizing alternatives to peat moss and other growth substrate materials is a hot topic in horticulture. Pine bark, mainly composed of organic substances such as cellulose, lignin, and resin, contains polysaccharides, lignin (a natural organic compound with antibacterial properties), and resin (a viscous substance with strong antibacterial and antioxidant effects). Furthermore, pine bark is an abundant source of agricultural waste and can be used as a raw material for growth substrates, especially since it is a common organic waste that can be applied to soilless horticulture. In addition, studies have found that perlite promotes rose growth.

[0003] Trichoderma harzianum can effectively promote plant growth, and it is inexpensive, safe to use, and has a good sustained effect. Simultaneously, Trichoderma harzianum can also act as a biocontrol agent, exhibiting antagonistic effects against various plant pathogenic fungi. Existing reports mainly focus on its growth-promoting and stress-resistance effects on plants such as wheat, corn, tobacco, passion fruit, tomato, cucumber, strawberry, and peas. However, its application in lilies has not been reported, especially its effect on the growth of lily bulbs, which remains unclear.

[0004] Currently, lily cultivation mainly uses peat moss, which, whether domestically produced or imported, is very costly. Developing alternatives to peat moss can effectively solve the cost problem and increase resilience to market risks. Secondly, viral diseases are the most important diseases affecting lilies and are also a problem that is difficult to solve with soil conditions. Using substrate cultivation can effectively solve the problem of continuous cropping year after year. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a composition and method for propagating lily bulbs, which uses pine bark as the main raw material to replace peat soil, thereby reducing costs. It solves the problem of continuous cropping obstacles in lily fields through substrate culture, and provides a new approach to the application of pine bark. At the same time, it utilizes Trichoderma harzianum to promote the enlargement of lily bulbs, which is beneficial to the healthier growth of the plants.

[0006] The objective of this invention is achieved through the following technical solution:

[0007] A composition for propagating lily bulbs includes a lily bulb propagation substrate and a lily bulb enlargement agent; the lily bulb propagation substrate comprises pine bark, perlite, and vermiculite in a ratio of 1–10:1–10:1–10; the lily bulb enlargement agent is a 5%–10% wettable powder solution of Trichoderma harzianum.

[0008] Furthermore, the preparation method of the above-mentioned lily bulb propagation substrate includes the following steps:

[0009] S1: Crush the pine bark to a particle size of 20-40mm;

[0010] S2: Mix the material obtained in step S1 with perlite and vermiculite in a mass ratio of 1-10:1-10:1-10;

[0011] S3: Control the moisture content of the material obtained in step S2 to 45%–55% and the pH value to 5–6 to obtain the lily seed bulb propagation substrate.

[0012] Furthermore, the bulk density of the aforementioned lily bulb propagation substrate is 0.13–0.25 g / cm³. 3 The total porosity is 65%–75%, and the EC value is <0.8 ms / cm.

[0013] The application of the above-mentioned composition in the propagation of lily bulbs.

[0014] Furthermore, the above composition can promote lily growth when used for lily bulb propagation.

[0015] Furthermore, the above-mentioned methods for promoting lily growth include any one or more of the following:

[0016] 1) Increase the fresh weight of the above-ground parts of lily plants;

[0017] 2) Increase the fresh weight of the underground part of the lily plant;

[0018] 3) Increase the root weight of lily plants;

[0019] 4) Increase the weight of the lily seed bulbs;

[0020] 5) Increase the circumference of lily bulbs.

[0021] A method for propagating lily bulbs involves soaking lily bulbs in the aforementioned lily bulb enlargement agent for 0.5 hours, and then planting them in the aforementioned lily bulb propagation substrate for cultivation.

[0022] Furthermore, the aforementioned lily bulbs are small bulbs that have undergone vernalization at 4℃, with a weight of 0.9g and a circumference of 3.44cm.

[0023] The significant advantages of this invention are:

[0024] 1) The lily bulb propagation substrate of this invention comprises pine bark, perlite, and vermiculite. This substrate has stable, loose, and breathable physical properties, with a pH controlled at 5-6. Combined with Trichoderma harzianum biological agents, it is highly suitable for the growth and development of lily bulbs. Compared with conventional lily cultivation substrates (peat soil), it exhibits superior growth characteristics and reduces costs by approximately 80% or more.

[0025] 2) This invention uses pine bark as the main raw material, providing a new approach to the application of pine bark and alleviating the pressure of agricultural waste on environmental pollution.

[0026] 3) This invention is the first to utilize Trichoderma harzianum biological agents on lily seed bulbs, which mainly shows a significant effect on promoting the growth of lily seed bulbs and a better effect on seed bulb enlargement.

[0027] 4) This invention plants lily bulbs in substrate bags filled with prepared lily bulb propagation substrate, allowing the lily bulb roots to wrap around the bulbs, forming a modular structure. This not only helps solve the problem of lily diseases and pests, but also solves the problem of continuous cropping obstacles in lily fields through substrate cultivation, resulting in healthier plant growth. Attached Figure Description

[0028] Figure 1 This is a flowchart of the substrate preparation and cultivation method of the present invention.

[0029] Figure 2 This is a schematic diagram of the lily bagging root system wrapping module of the present invention. Detailed Implementation

[0030] To make the content of this invention easier to understand, the technical solution of this invention will be further described below with reference to specific embodiments, but this invention is not limited thereto.

[0031] The pine bark used in this embodiment of the invention is the bark of Podocarpus macrophyllus.

[0032] The Trichoderma harzianum wettable powder used in this embodiment of the invention was purchased from BioVols Inc., USA, with an active ingredient content of 300 million CFU / gram.

[0033] The lily bulbs used in this embodiment of the invention are small bulbs that have undergone vernalization at 4°C, with a mass of 0.9g and a circumference of 3.44cm.

[0034] In this embodiment of the invention, the planting depth of the lily bulbs in the lily bulb propagation substrate is 8cm, the planting density is one plant per substrate bag, and the cultivation conditions are greenhouse and seedbed cultivation.

[0035] Example 1:

[0036] This embodiment provides a composition for lily bulb propagation, the composition comprising a lily bulb propagation substrate; the lily bulb propagation substrate comprises pine bark, perlite, and vermiculite, wherein the mass ratio of pine bark, perlite, and vermiculite in the substrate is 1:1:1. The preparation method of the lily bulb propagation substrate includes:

[0037] S1: Crush the pine bark to a particle size of 20-40mm;

[0038] S2: Mix the material obtained in step S1 with perlite and vermiculite in a mass ratio of 1:1:1;

[0039] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 5.51 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.321 g / cm³. 3 The total porosity is 83.5%, and the EC value is 0.23 mS / cm.

[0040] Lily bulbs were planted in the lily bulb propagation substrate for cultivation, and at maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0041] Example 2:

[0042] This embodiment provides a composition for lily bulb propagation, comprising a lily bulb propagation substrate and a lily bulb enlargement agent; the lily bulb propagation substrate comprises pine bark, perlite, and vermiculite, wherein the mass ratio of pine bark, perlite, and vermiculite in the substrate is 1:1:1; the lily bulb enlargement agent is a 0.5% (w / w) wettable powder solution of *Trichoderma harzianum*. The preparation method of the lily bulb propagation substrate includes:

[0043] S1: Crush the pine bark to a particle size of 20-40mm;

[0044] S2: Mix the material obtained in step S1 with perlite and vermiculite in a mass ratio of 1:1:1;

[0045] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 5.51 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.331 g / cm³. 3 The total porosity was 83.2%, and the EC value was 0.21 mS / cm.

[0046] The lily bulbs were soaked in the lily bulb enlargement agent at room temperature for 0.5 hours, and then planted in the lily bulb propagation substrate for cultivation. At maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0047] Example 3

[0048] This embodiment provides a composition for lily bulb propagation, the composition comprising a lily bulb propagation substrate; the lily bulb propagation substrate comprises pine bark, sawdust, and vermiculite, wherein the mass ratio of pine bark, sawdust, and vermiculite in the substrate is 1:1:1. The preparation method of the lily bulb propagation substrate includes:

[0049] S1: Crush the pine bark to a particle size of 20-40mm;

[0050] S2: Mix the material obtained in step S1 with sawdust and vermiculite in a mass ratio of 1:1:1;

[0051] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 5.73 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.221 g / cm³. 3 The total porosity is 81.5%, and the EC value is 0.24 mS / cm.

[0052] Lily bulbs were planted in the lily bulb propagation substrate for cultivation, and at maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0053] Example 4

[0054] This embodiment provides a composition for lily bulb propagation, comprising a lily bulb propagation substrate and a lily bulb enlargement agent; the lily bulb propagation substrate comprises pine bark, sawdust, and vermiculite, wherein the mass ratio of pine bark, sawdust, and vermiculite in the substrate is 1:1:1; the lily bulb enlargement agent is a 0.5% (w / w) wettable powder solution of *Trichoderma harzianum*. The preparation method of the lily bulb propagation substrate includes:

[0055] S1: Crush the pine bark to a particle size of 20-40mm;

[0056] S2: Mix the material obtained in step S1 with sawdust and vermiculite in a mass ratio of 1:1:1;

[0057] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 5.73 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.225 g / cm³. 3 The total porosity is 80.5%, and the EC value is 0.25 mS / cm.

[0058] The lily bulbs were soaked in the lily bulb enlargement agent at room temperature for 0.5 hours, and then planted in the lily bulb propagation substrate for cultivation. At maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0059] Example 5

[0060] This embodiment provides a composition for lily bulb propagation, the composition comprising a lily bulb propagation substrate; the lily bulb propagation substrate comprises pine bark, sawdust, and perlite, wherein the mass ratio of pine bark, sawdust, and perlite in the substrate is 1:1:1. The preparation method of the lily bulb propagation substrate includes:

[0061] S1: Crush the pine bark to a particle size of 20-40mm;

[0062] S2: Mix the material obtained in step S1 with sawdust and perlite in a mass ratio of 1:1:1;

[0063] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 6.08 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.251 g / cm³. 3 The total porosity is 81.5%, and the EC value is 0.225 mS / cm.

[0064] Lily bulbs were planted in the lily bulb propagation substrate for cultivation, and at maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0065] Example 6

[0066] This embodiment provides a composition for lily bulb propagation, comprising a lily bulb propagation substrate and a lily bulb enlargement agent; the lily bulb propagation substrate comprises pine bark, sawdust, and perlite, wherein the mass ratio of pine bark, sawdust, and perlite in the substrate is 1:1:1; the lily bulb enlargement agent is a 0.5% (w / w) wettable powder solution of *Trichoderma harzianum*. The preparation method of the lily bulb propagation substrate includes:

[0067] S1: Crush the pine bark to a particle size of 20-40mm;

[0068] S2: Mix the material obtained in step S1 with sawdust and perlite in a mass ratio of 1:1:1;

[0069] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 6.08 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.241 g / cm³. 3The total porosity is 81.0%, and the EC value is 0.215 mS / cm.

[0070] The lily bulbs were soaked in the lily bulb enlargement agent at room temperature for 0.5 hours, and then planted in the lily bulb propagation substrate for cultivation. At maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0071] Example 7

[0072] This embodiment provides a composition for lily bulb propagation, the composition comprising a lily bulb propagation substrate; the lily bulb propagation substrate comprises pine bark, peat moss, and perlite, wherein the mass ratio of pine bark, peat moss, and perlite in the substrate is 1:1:1. The preparation method of the lily bulb propagation substrate includes:

[0073] S1: Crush the pine bark to a particle size of 20-40mm;

[0074] S2: Mix the material obtained in step S1 with peat moss and perlite in a mass ratio of 1:1:1;

[0075] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 4.74 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.261 g / cm³. 3 The total porosity is 82.5%, and the EC value is 0.25 mS / cm.

[0076] Lily bulbs were planted in the lily bulb propagation substrate for cultivation, and at maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0077] Example 8

[0078] This embodiment provides a composition for lily bulb propagation, comprising a lily bulb propagation substrate and a lily bulb enlargement agent; the lily bulb propagation substrate comprises pine bark, peat moss, and perlite, wherein the mass ratio of pine bark, peat moss, and perlite in the substrate is 1:1:1; the lily bulb enlargement agent is a 0.5% (w / w) wettable powder solution of *Trichoderma harzianum*. The preparation method of the lily bulb propagation substrate includes:

[0079] S1: Crush the pine bark to a particle size of 20-40mm;

[0080] S2: Mix the material obtained in step S1 with peat moss and perlite in a mass ratio of 1:1:1;

[0081] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 4.74 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.261 g / cm³. 3 The total porosity is 80.3%, and the EC value is 0.24 mS / cm.

[0082] The lily bulbs were soaked in the lily bulb enlargement agent at room temperature for 0.5 hours, and then planted in the lily bulb propagation substrate for cultivation. At maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0083] Example 9

[0084] This embodiment provides a composition for lily bulb propagation, the composition comprising a lily bulb propagation substrate; the lily bulb propagation substrate comprises corn silk, pine bark, and peat moss, wherein the mass ratio of corn silk, pine bark, and peat moss in the substrate is 1:1:1. The preparation method of the lily bulb propagation substrate includes:

[0085] S1: Crush the corn silk and pine bark into particles of 20-40 mm respectively;

[0086] S2: Mix the corn silk material, pine bark material and peat soil obtained in step S1 at a mass ratio of 1:1:1;

[0087] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 5.46 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.271 g / cm³. 3 The total porosity is 81.5%, and the EC value is 0.223 mS / cm.

[0088] Lily bulbs were planted in the lily bulb propagation substrate for cultivation, and at maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0089] Example 10

[0090] This embodiment provides a composition for lily bulb propagation, comprising a lily bulb propagation substrate and a lily bulb enlargement agent; the lily bulb propagation substrate comprises corn silk, pine bark, and peat moss, wherein the mass ratio of corn silk, pine bark, and peat moss in the substrate is 1:1:1; the lily bulb enlargement agent is a 0.5% (w / w) wettable powder solution of *Trichoderma harzianum*. The preparation method of the lily bulb propagation substrate includes:

[0091] S1: Crush the corn silk and pine bark into particles of 20-40 mm respectively;

[0092] S2: Mix the corn silk material, pine bark material and peat soil obtained in step S1 at a mass ratio of 1:1:1;

[0093] S3: The material obtained in step S2 is subjected to water absorption treatment until the moisture content is controlled at 50%, and the pH value is adjusted to 5.46 using citric acid to obtain the lily seed bulb propagation substrate with a bulk density of 0.231 g / cm³. 3 The total porosity is 81.2%, and the EC value is 0.24 mS / cm.

[0094] The lily bulbs were soaked in the lily bulb enlargement agent at room temperature for 0.5 hours, and then planted in the lily bulb propagation substrate for cultivation. At maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0095] Example 11

[0096] This embodiment provides a composition for the propagation of lily bulbs, the composition comprising a lily bulb propagation substrate; the lily bulb propagation substrate comprises peat moss with a moisture content controlled at 50%, a pH value controlled at 5.45, and a bulk density of 0.291 g / cm³. 3 The total porosity is 81.5%, and the EC value is 0.228 mS / cm.

[0097] Lily bulbs were planted in the lily bulb propagation substrate for cultivation, and at maturity, the bolting rate, above-ground fresh weight, underground fresh weight, root weight, bulb weight, and bulb circumference were measured.

[0098] The results are shown in the table below. The compositions provided in Examples 1 and 2 of this invention are more beneficial to the growth and development of lilies.

[0099]

[0100] Note: Different lowercase letters in the same column indicate significant differences at the 0.05 level, and different uppercase letters in the same column indicate highly significant differences at the 0.01 level.

[0101] In addition, after soaking the lily bulbs in the lily bulb enlargement agent described in Example 2 at room temperature for 0.5 hours, they were planted in substrate bags filled with the lily bulb propagation substrate described in Example 2 for cultivation. The lily bulb roots could wrap around the bulbs, forming modular structures. Figure 2 ).

[0102] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A composition for lily bulb breeding, characterized in that: It comprises a lily bulb propagation matrix and a lily bulb expansion agent; the lily bulb propagation matrix comprises pine bark, perlite and vermiculite, and the mass ratio of the pine bark, perlite and vermiculite in the matrix is ​​1-10:1-10:1-10; the lily bulb expansion agent is a 5%-10% mass fraction of Trichoderma harzianum wettable powder solution; The method for preparing the lily bulb breeding matrix comprises the following steps: S1: crush the pine bark to a particle size of 20-40 mm; S2: Mixing the material obtained in step S1 with perlite and vermiculite in a mass ratio of 1-10:1-10:1-10; S3: Controlling the moisture content of the material obtained in step S2 to 45%-55% and the pH value to 5-6 to obtain a lily bulb breeding matrix; The bulk density of the lily bulb breeding matrix is ​​0.13-0.25 g / cm 3 , the total porosity is 65%~75%, and the EC value is <0.8ms / cm.

2. Use of the composition according to claim 1 in lily bulb breeding, characterized in that: The composition can be used for lily bulb propagation to promote lily growth; the lily growth promotion is any one or more of the following: 1) Increase the aboveground fresh weight of lily plants; 2) Increase the fresh weight of underground parts of lily plants; 3) Increase the root weight of lily plants; 4) Increase the weight of lily seed bulbs; 5) Increase the circumference of lily bulbs.

3. A lily bulb breeding method, characterized in that: The lily bulbs are soaked in the lily bulb enlargement agent described in claim 1 for 0.5 hours, and then planted in the lily bulb breeding matrix described in claim 1.

4. The lily bulb breeding method according to claim 3, characterized in that: The lily seed ball is a small bulb ball that has been vernalized at 4°C, has a mass of 0.9g and a circumference of 3.44cm.

Images