Use of n-acetylneuraminic acid in synergy with acetyl hexapeptide-8

Abstract

This invention relates to the field of cosmetic technology, disclosing the application of N-acetylneuraminic acid in synergistically enhancing the skincare effects of acetyl hexapeptide-8. By combining N-acetylneuraminic acid and acetyl hexapeptide-8 in the mass ratios provided in this application, a synergistic effect on the anti-aging effects of acetyl hexapeptide-8 can be achieved, increasing the content of collagen and elastin in the skin. However, when the ratio of N-acetylneuraminic acid to acetyl hexapeptide-8 is adjusted outside the range provided in this application, the synergistic effect disappears. Therefore, by using N-acetylneuraminic acid and acetyl tetrapeptide-2 synergistically in cosmetics and / or skincare products according to the compounding ratios provided in this application, significant anti-aging effects can be achieved at lower application amounts, improving skin firmness and elasticity, and reducing the formation of wrinkles.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, in particular to the application of N-acetylneuraminic acid in synergistically enhancing the skin care effect of acetyl hexapeptide-8. BACKGROUND

[0002] Bioactive peptide compounds have unique advantages of high biological activity, easy absorption, safety and non-toxicity, and in recent years, their physiological functions and biological effects in the research topics of beauty care and anti-aging have gradually attracted attention. The biological activity of polypeptides depends on their amino acid composition and sequence, and almost all physiological processes in the human body are regulated by polypeptides or proteins composed of specific amino acid sequences. Adding peptides to cosmetics can help improve and repair various skin problems, but currently there are more reports on the application of single peptide active substances, and less research and development on the combination of multiple peptides.

[0003] Aging, also known as senescence, usually refers to the phenomenon that the body function declines, the internal environment stability decreases, and the structure gradually degenerates with the increase of age under normal circumstances after the biological development matures. Skin aging is part of human aging and its external manifestation. The characteristics are: sagging, roughness, wrinkle formation, pigment disorders, etc. Among them, the main reason for the formation of skin wrinkles is related to the progressive atrophy of the dermis. The dermis is mainly composed of extracellular matrix and fibroblasts. During the aging process, collagen and elastic fibers, which are the main components of the extracellular matrix, become fragmented and the total amount decreases, causing wrinkles. There are many types of collagen, which are the structure and scaffold of tissues such as bone, ligament, cornea, and blood vessel wall. Among them, type I collagen accounts for about 85% of the total collagen in the human body and is the most abundant protein in vertebrates, which plays a key role in skin tightening and elasticity. Elastin is the main component of elastic fibers, although elastin only accounts for about 2% of the total protein in the dermis, but it plays an important role in skin elasticity, and the decrease of elastin content in the skin is the main reason for the generation of sagging, drooping and fine wrinkles in skin aging. Therefore, continuously supplementing or enhancing the synthesis of collagen and elastin is an effective way to resist aging and wrinkle formation.

[0004] Acetyl hexapeptide-8 is a neurotransmitter inhibitory peptide that can inhibit the release of neurotransmitter acetylcholine, reduce muscle contraction, and thus reduce the formation of dynamic wrinkles and expression wrinkles. Studies have shown that acetyl hexapeptide-8 can increase the synthesis of type I collagen, improve skin firmness and elasticity, and reduce wrinkle formation. SUMMARY

[0005] The purpose of the present application is to provide the use of N-acetylneuraminic acid in synergistically enhancing the skin care effect of acetyl hexapeptide-8.

[0006] The first aspect of the present invention provides the application of N-acetylneuraminic acid to synergistically enhance the skin care effects of acetyl hexapeptide-8, wherein the mass ratio of N-acetylneuraminic acid to acetyl hexapeptide-8 is (1-4):1.

[0007] Preferably, in the application of N-acetylneuraminic acid synergistic acetyl hexapeptide-8 provided by the present invention, the mass ratio of N-acetylneuraminic acid to acetyl hexapeptide-8 is (1-2):1.

[0008] Specifically, the N-acetylneuraminic acid synergistically enhances the efficacy of acetyl hexapeptide-8 in promoting skin collagen and elastin.

[0009] A second aspect of the present invention provides a polypeptide composition having a synergistic effect, comprising, by weight: N-acetylneuraminic acid: 1 to 4 parts; acetyl hexapeptide-8: 1 part.

[0010] Preferably, the polypeptide composition with synergistic effect provided by the present invention comprises, by weight, 1-2 parts of N-acetylneuraminic acid and 1 part of acetyl hexapeptide-8.

[0011] A third aspect of the present invention provides the use of the polypeptide composition described in the second aspect of the present invention in cosmetics and / or skin care products.

[0012] In the applications provided by this invention, the cosmetics and / or skin care products are applied to the outer surface of the skin.

[0013] Preferably, in the application provided by the present invention, the cosmetics and / or skin care products are selected from one or more of eye cream, face cream, serum, lotion, and facial mask essence.

[0014] Preferably, in the application provided by the present invention, the polypeptide composition is dispersed as an additive in a matrix raw material, wherein the matrix raw material is selected from one or more of oily raw materials, powdery raw materials, gel-like raw materials, and solvent raw materials.

[0015] Preferably, in the applications provided by the present invention, the cosmetics and / or skin care products further include one or more of the following: surfactants, fragrances and flavorings, pigments, moisturizers, preservatives, antioxidants, ultraviolet absorbers, chelating agents, astringents, penetration enhancers, and nutritional additives.

[0016] Compared to existing technologies, the applicant proposes that using N-acetylneuraminic acid and acetyl hexapeptide-8 in the mass ratio range provided in this application can achieve a synergistic effect on the anti-aging efficacy of acetyl hexapeptide-8, significantly promoting skin collagen and elastin production. However, when the ratio of N-acetylneuraminic acid to acetyl hexapeptide-8 is adjusted outside the range provided in this application, the synergistic effect disappears. Therefore, using N-acetylneuraminic acid and acetyl tetrapeptide-2 in cosmetics and / or skincare products in accordance with the compounding ratio range provided in this application can exert a significant anti-aging effect on the skin at a relatively low dosage, effectively increasing skin collagen and elastin production, improving skin firmness and elasticity, and reducing wrinkles. Attached Figure Description

[0017] Figure 1 A schematic diagram of the synergistic effect assay of N-acetylneuraminic acid and acetyl hexapeptide-8;

[0018] Figure 2 Comparison of the effects of N-acetylneuraminic acid and acetyl hexapeptide-8 alone and in combination at different ratios on Col-I mRNA expression in HFF-1 cells;

[0019] Figure 3 Comparison of the effects of N-acetylneuraminic acid and acetyl hexapeptide-8 alone and in combination at different ratios on Col-IV mRNA expression in HFF-1 cells;

[0020] Figure 4 A comparative diagram showing the effects of N-acetylneuraminic acid and acetyl hexapeptide-8 alone and in combination at different ratios on Elastin mRNA expression in HFF-1 cells. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this invention clearer, the various embodiments of this invention will be described in detail below. However, those skilled in the art will understand that many technical details have been provided in the various embodiments of this invention to facilitate a better understanding of this application. However, the technical solutions claimed in this application can be implemented even without these technical details and various variations and modifications based on the following embodiments.

[0022] Some embodiments of the present invention provide the application of N-acetylneuraminic acid synergistically enhancing the skin care effects of acetyl hexapeptide-8. Specifically, the N-acetylneuraminic acid synergistically enhances the skin care effects of acetyl hexapeptide-8 in improving skin collagen and elastin, wherein the mass ratio of the N-acetylneuraminic acid to the acetyl hexapeptide-8 is (0.5-2):1.

[0023] In some embodiments of the present invention, the mass ratio of N-acetylneuraminic acid to acetyl hexapeptide-8 is (1-2):1.

[0024] Some embodiments of the present invention provide a polypeptide composition with synergistic effects, comprising, by weight: N-acetylneuraminic acid: 0.5 to 2 parts; acetyl hexapeptide-8: 1 part.

[0025] In the polypeptide composition with synergistic effect provided in some embodiments of the present invention, by weight, it comprises: N-acetylneuraminic acid: 1 to 2 parts; acetyl hexapeptide-8: 1 part.

[0026] Some embodiments of the present invention also provide the use of the above-described polypeptide compositions in cosmetics and / or skin care products.

[0027] The polypeptide compositions provided by embodiments of the present invention can be applied to cosmetics and / or skin care products, which are applied to the outer surface of the skin.

[0028] In some embodiments of the present invention, cosmetics and / or skin care products using the polypeptide composition are selected from one or more of eye creams, face creams, serums, lotions, and facial mask essences.

[0029] In some embodiments of the present invention, the polypeptide composition is dispersed as an additive in a matrix raw material, wherein the matrix raw material is selected from one or more of oily raw materials, powdery raw materials, gel-like raw materials, and solvent raw materials.

[0030] The oily raw material can be any one or a combination of the following matrix raw materials: soybean oil, olive oil, almond oil, castor oil, peanut oil, cottonseed oil, tea seed oil, jojoba oil, avocado oil, coconut oil, palm oil, cocoa butter, rice bran oil, evening primrose oil, wheat germ oil, corn germ oil, palm wax, wood wax, mink oil, turtle oil, snake oil, egg yolk oil, beef tallow, horse tallow, lard, deer tallow, lecithin, lanolin, beeswax, cetacean wax, shellac wax, paraffin wax, petrolatum, microcrystalline wax, ceresin, squalane, lanolin wax, lanolin alcohol, and lanolin. Alcohol esters, acetylated lanolin, acetylated lanolin alcohol, lanolin acid, lanolin esters, polyoxyethylene lanolin, polyoxyethylene lanolin alcohol ether, polyoxypropylene lanolin alcohol ether, hydrogenated lanolin, alkoxylated hydrogenated lanolin, silicone oil (e.g., dimethyl silicone oil, octamethyl silicone oil, methylphenyl polysiloxane), polysiloxane-polyoxyalkyl block copolymer, methyl hydrogen silicone oil, Cn fatty acids and their esters, Cn fatty alcohols and their esters; wherein n ≥ 10, and preferably 10-24, more preferably 12-20, such as 14, 16, 17, 18.

[0031] The Cn fatty acids include: lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, erucic acid, and decanoic acid.

[0032] The Cn fatty alcohols include: lauryl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, and coconut oil alcohol.

[0033] The Cn fatty acid esters and Cn fatty alcohol esters include: isopropyl myristate, myristyl myristate, tetradecyl lactate, hexadecyl lactate, isopropyl palmitate, palm isooctyl ester, butyl stearate, isooctyl stearate, monoglyceride stearate, polyethylene glycol stearate, decyl oleate, glyceryl caprylate, glyceryl caprylate, and trioleate.

[0034] The powdery raw material can be any one or a combination of the following matrix raw materials: mica, magnesium carbonate, titanium dioxide, zinc dioxide, calcium hydroxide, calcium carbonate, kaolin, calcium stearate, zinc stearate, magnesium stearate, talc, silicon dioxide, aluminum hydroxide, calcium pyrophosphate, calcium bicarbonate, and bentonite.

[0035] Among them, gelatinous raw materials swell into colloids in water, which can bind solid powder raw materials together and play an emulsifying role in emulsions or suspensions. In addition, they also have thickening or gelling effects. Commonly used gelatinous raw materials include plant gels, animal gelatin, etc.

[0036] The solvent raw material can be any one or a combination of the following matrix raw materials: water, ethanol, propanol, isopropanol, isobutanol, ethylene glycol, 1,2-propanediol, cyclohexane, dichlorodifluoromethane, tetrafluorodichloroethane, ethylene glycol monoethyl ether, diethylene glycol monoethyl ether, acetone, methyl isobutyl ketone, ethyl acetate, butyl acetate, amyl acetate, and dibutyl phthalate.

[0037] In some embodiments of the present invention, cosmetics and / or skin care products using the polypeptide composition further include one or more of the following: surfactants, fragrances and flavorings, pigments, moisturizers, preservatives, antioxidants, ultraviolet absorbers, chelating agents, astringents, penetration enhancers, and nutritional additives.

[0038] The surfactant may be: 1) anionic surfactants, such as: C12-14 fatty alcohol ammonium sulfate, C12-14 fatty alcohol polyoxyethylene ether carboxylate, diethanolamine dodecyl sulfate, C12-14 fatty alcohol polyoxyethylene ether ammonium sulfate, triethanolamine dodecyl sulfate, sodium monophthalate monoester of alcohol ether, acylated peptide, sodium monoethanolamide laurate sulfate, n-octyl alcohol polyoxypropylene ether succinate monoester sulfonate, cetyl alcohol succinate monoester sulfonate, disodium sulfosuccinate monoester, disodium octadecyl succinate monoester, disodium sulfosuccinate monoester of alcohol ether, fatty alcohol polyoxyethylene ether (sodium) sulfosuccinate monoester ammonium salt, oleamide sulfosuccinate monoester disodium salt, N - Acylglutamate potassium salt, pyrrolidone carboxylate sodium salt, sodium polyacrylate, fatty alcohol polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate monoester and its salt, fatty alcohol polyoxyethylene ether phosphate monoester ethanolamine salt, alkylphenol polyoxyethylene ether phosphate monoester and its salt, nonylphenol ether phosphate monoester ethanolamine salt, alkyl phosphate salt, phthalic acid monolaurate salt, zinc undecenoate, anionic amino acid surfactants, N-C12-18 acylglutamate sodium salt, N-lauroyl L-alanine sodium salt, N-stearoylglutamate monosodium salt, N-cocoylglutamate monosodium salt, N-mixed fatty acylglutamate monosodium salt, long-chain alkyl aryl ether sulfonate sodium TH; 2) Nonionic surfactants. Examples include: oleyl alcohol polyoxyethylene ethers (such as emulsifier VO series), alkylphenol polyoxyethylene ethers (such as emulsifier OPE series), polyoxyethylene stearate, ethylene glycol monostearate, ethylene glycol distearate, polyethylene glycol monostearate, polyethylene glycol distearate, polyethylene glycol dilaurate, glyceryl distearate, glyceryl tristearate, sucrose stearate, polyoxyethylene glycerol ether monostearate, N-lauroyl glutamate dialkyl ester, benzoic acid fatty alcohol ester, sorbitan monofatty acid ester (Span, such as Span-20, Span-60, Span-65, Span-80, Span-83, Span-85), polyoxyethylene ether sorbitan monofatty acid ester (Tween). en, such as Tween-20, Tween-40, Tween-60, Tween-61, Tween-80), diethanolamine, triethanolamine, fatty acid monoethanolamides (such as coconut oil monoethanolamide, laurate monoethanolamide, palmitic acid monoethanolamide), coconut oil diethanolamide, laurate diethanolamide, methyl glucoside stearate, polyoxyethylene methyl glucoside stearate, ethylene glycol glucoside stearate, propylene glycol glucoside stearate, C8-16 alkyl glucoside, glyceryl glucoside stearate, lauryl ether monoester of succinate, lauryl glycerol polyoxyethylene ether, mannitol stearate, polyoxyethylene mink oil, methyl mink oil, isopropyl mink oil.3) Cationic surfactants, such as C12-18 alkyltrialkylammonium chloride (e.g., hexadecyl dimethyl alkylammonium chloride, octadecyl trimethylammonium chloride, hexadecyl trimethylammonium chloride), oleamidopropyl dihydroxypropyl dimethylammonium chloride, dihydroxypropyl dimethyl dodecylammonium chloride, dimethyl diallyl ammonium chloride / acrylamide copolymer, oleamidopropyl-2,3-dihydroxypropyl dimethylammonium chloride, ethyl oleamidopropyl dimethyl ethylammonium sulfate, bis(C12-18) dimethylammonium chloride, polyquaternium-11 conditioning agent, M-505 polyquaternium-7 conditioning agent, mink oleamidopropylamine-chitosan, mink oleamidopropylamine-hydrolyzed protein, cationic protein peptides, guar gum-hydroxypropyltrimethylammonium chloride, etc. Ammonium chloride salts, polycellulose ether quaternary ammonium salts, triethanolamine monooleate, DNP series; 4) zwitterionic surfactants, such as C12-18 alkyl dihydroxyethyl betaine, N-C12-18 alkyl-N-(2-hydroxyethyl)-N-(2-formamidoethyl)ammonium acetate, C12-18 alkoxyhydroxypropyl betaine, oxidized cocamidopropylamine, sodium cocoyl hydroxyethyl sulfonate, oxidized octadecyl dimethylamine, cocoyl diethanolamine amine oxide, N-alkyl-β-aminopropionyl diethanolamine, oxidized lauroyl propylamine, N-C12-18 acylglutamic acid, hydroxyethyl decanoic acid imidazoline betaine, hydroxyethyl myristate imidazoline betaine, hydroxyethyl palmitic imidazoline betaine.

[0039] The fragrances and flavorings may be selected from: 1) natural flavorings, such as wormwood oil, eucalyptus oil, star anise oil, white lemon oil, white clove extract, clove oil, magnolia oil, magnolia extract, magnolia leaf oil, cypress oil, peppermint oil, atractylodes stearate, mesona extract, orange leaf oil, clove basil oil, jasmine extract, wintergreen oil, angelica oil, angelica extract, lichenic acid, sandalwood oil, bitter orange flower oil, bitter orange leaf oil, hyacinth extract, sweetgum extract, linalool oil, sassafras oil, spikenard oil, osmanthus extract, mandarin orange oil, carrot seed oil, castor oil, ambergris oil, galangal oil, patchouli oil, artemisia oil, Murraya paniculata extract, acacia extract, ginger oil, mandarin orange oil, peppermint oil, chrysanthemum extract, cumin oil, ambergris, spearmint oil, civet, wintersweet oil, bellflower oil, etc. Orchid extract, costus root oil, milan flower oil, rose leaf oil, rose oil, jasmine extract, oyster oil, dark red extract, dark red oil, lemon eucalyptus leaf oil, lemon oil, Murraya paniculata extract, nanmu leaf oil, celery seed oil, nutmeg oil, cinnamon oil, litsea cubeba oil, mink balsam tincture, narcissus extract, pine needle oil, tree moss extract, jasmine extract, musk, muskrat balsam, sweet orange oil, sandalwood oil, evening primrose. Tuberose oil, tuberose extract, marigold essential oil, fennel oil, blood cypress oil, lemongrass oil, bay leaf oil, carnation oil, perilla oil, fragrant perilla oil, cyperus oil, bergamot and peppermint oil, vetiver oil, sesame oil, sesame extract, raspberry rose oil, raspberry rose extract, lavender oil, bay leaf oil, ylang-ylang oil, coriander seed oil, fish mint oil, rockrose extract, rock... 1) Cinnamon oil, grapefruit peel oil, iris oil, tobacco flower oil, citron leaf oil, gardenia flower extract, amorpha fruticosa oil, violet leaf extract, guaiacol, 4-methylguaiacol, 4-ethylguaiacol, natural vanillin, camphor; 2) Synthetic fragrances, such as limonene, longifolene, ethyl caryophyllene, isolongifolene, isolongifolene ketone, isolongifolene ketone, bromostyraxene, diphenylmethane, diphenyl ether, m-methyl diphenyl ether, eugenol, isoeugenol, ethyl naphthyl methyl ether, ethyl naphthyl ethyl ether, p-methyl anisole, isoeugenol benzyl ether, rose ether, sandalwood ether, narcissus ether (or jasmine ether), ambergris ether, methyl cedarwood ether, epoxy cedarwood ether, elintall, elintall N, 3-hexenol, decanol, lauryl alcohol, benzyl alcohol, phenethyl alcohol, p-methoxybenzyl alcohol, cinnamyl alcohol. Dimethylbenzylmethanol, Geraniol, Nerol, Linalool, Rose alcohol, Terpineol, Citronellol, Racemic Menthol, Menthol, Borneol, Cypressol, Cypressone, Methyl Cypressone, 3,4-Dioxomethylenebenzyl alcohol, α-Methyl-3,4-Dihydromethylenephenylpropanal, 2-tert-butyl-4-methylcyclohexanol, Perillyl alcohol, Santalol, 3-Methyl-5-(2',2',3'-trimethylcyclopenten-1'-yl)pent-2-ol, Benzaldehyde, Acetophenone, Phenylacetic acid, p-Methylacetophenone, Benzophenone, Lauraldehyde, Cinnamaldehyde, Cinnamic acid, α-pentyl-β-phenylpropenal, α-Phenylaceal, Lily of the Valley Aldehyde, Neolily of the Valley Aldehyde, Rabbit Ear Aldehyde, 3,4-Dioxomethylenebenzaldehyde, 3,7-Dimethyl-6-octenal, 3,7-Dimethyl-7-hydroxyoctanal, citral, ethyl-cyclocitral, laval, citric acid, 4-hydroxy-3-methoxybenzaldehyde, α-dihydro-draconone, β-draconone, ionone, 6-methyl-ionone, nerone, carvone, piperylacetone, raspberry ketone, herbicide, cis-jasmone, heptanal ethylene glycol acetal, phenylacetaldehyde dimethyl acetal, octanal dimethyl acetal, α-pentylcinnamaldehyde dimethyl acetal, citral dimethyl acetal, citral diethyl acetal, anisaldehyde dimethyl acetal, mushroom aldehyde, chlorophyll Hyacin, apple ester, jeep ketal, geraniol formate, citronellol formate, affrmann ester, leaf alcohol acetate, benzyl acetate, phenethyl acetate, cinnamon ester, terpineol acetate, borneol acetate, linalyl acetate, geraniol acetate, p-tert-butylcyclohexyl acetate, o-tert-butylcyclohexyl acetate, vetiver acetate, succinate acetate, affrmann acetate, decahydronaphthyl acetate, cypress ester, geraniol propionate, trichloromethylbenzyl acetate, benzyl butyrate, leaf alcohol butyrate, geraniol butyrate, 2-methylvaleric acid phenylethyl Esters, p-cresol isobutyrate, methyl 2-nonenoate, ethyl benzoate, methyl benzoate, leaf benzoate, benzyl benzoate, ethyl phenylacetate, p-cresol phenylacetate, ethyl cinnamate, butyl salicylate, isobutyl salicylate, isoamyl salicylate, leaf benzoate, hexyl salicylate, benzyl salicylate, phenethyl salicylate, cyclohexyl salicylate, jasmine, jasmine ester, methyl 2-pentylcyclopentanone acetate, nerolidin, γ-nonanolide, γ-undecyl lactone, coumarin, indole, 3-methylindole, 3,7- Dimethyl-2,6-octadienonitrile, p-methoxybenzonitrile, xylene musk, ketone musk, 2,6-dinitro-3-methoxy-4-tert-butyltoluene, 1,1-dimethyl-4-acetyl-6-tert-butylindane, hexamethyltricycloisocyanuric musk, 1,1,2,3,3,6-hexamethyl-5-acetylindane, musk-T, musk-M, musk-L, musk-F, 11-oxacyclohexadecalactone, tuna musk; 3) fragrances, rose fragrance, jasmine fragrance, sandalwood fragrance, magnolia fragrance.

[0040] The pigment may be selected from one or more of the following components: zirconium dioxide, lead acetate, silver nitrate, chlorophyll, copper chlorophyll, ferrous ferrocyanide, guanine, fast red, food red I, reduced red I, carmine, edible bayberry red, edible cherry red, food red 17, amaranth red, fast green, food yellow 3, edible tartrazine, edible Sudan yellow, edible indigo, food blue 2, fluorescent pink, β-carotene, henna, gardenia red, gardenia green, gardenia blue, gardenia yellow, titanium mica pearlescent pigment, iron oxide red, iron oxide yellow, iron oxide black, chromium oxide green, bismuth oxychloride, basic pink, cinnabar R, quinoline yellow, lithospermum erythrorhizon, shellac red, ultramarine, acid red 87, acid green 25, acid fluorescent yellow, acid orange 7, golden red 53, Litholite BK, Litholite 2R, permanent orange.

[0041] The preservative may be selected from one or more of the following components: sorbic acid and its salts, propionate, p-hydroxybenzoate, benzyl alcohol, benzoic acid and its salts, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, Kathon, imidazolidinyl urea, dehydroacetic acid and its salts, sodium sulfite, sodium bisulfite, sodium metabisulfite, 2,2-bromo-2-nitro-1,3-propanediol (Brobol), isoascorbic acid and its salts, kojic acid and its esters, azelaic acid, usnic acid, and resorcinol.

[0042] The antioxidant may be one or a combination of the following components: SOD, butylated hydroxyanisole, gallate, ascorbic acid and its salts, isoascorbic acid and its salts, sitosterol, rutin, and tocopherol.

[0043] The moisturizer may be selected from one or more of the following components: 1,2-butanediol, sorbitol, xylitol, glycerin, lactate, polyethylene glycol, DL-pyrrolidone carboxylate, D-glucose, kojic acid and its esters, uric acid, orotic acid, pectic acid, laminine, collagen, trehalose, and 12-hydroxystearic acid cholesterol ester.

[0044] The ultraviolet absorber may be selected from one or more of the following components: pyridoxine hydrochloride, kojic acid and its esters, rutin, aloe-emodin, caffeine, hesperidin, 2-ethylhexyl salicylate, 2-ethylhexyl p-dimethylaminobenzoate, 2-ethylhexyl p-methoxycinnamate, phenyl salicylate, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, and 2-hydroxy-4-octyloxybenzophenone.

[0045] The chelating agent may be one or a combination of the following components: disodium EDTA, zinc dipyridylthione, and sodium thiosulfate.

[0046] The astringent may be one or a combination of the following components: zinc phenol-p-sulfonate, aluminum hydroxychloride, aluminum sulfate, potassium aluminum sulfate, zinc sulfate, aluminum chloride, zinc chloride, and carmine.

[0047] The penetration enhancer may be one or a combination of the following components: dimethyl sulfoxide, 1-dodecylazine-2-one, and 2-aminoethanol.

[0048] The pH adjuster may be one or a combination of the following components: tartaric acid, lactic acid, boric acid, and succinic acid.

[0049] The nutritional additives may be selected from one or more of the following components: chitosan, chitosan, silk peptides, silk fibroin, lecithin, epidermal growth factor, trehalose, lactose, pearl powder, collagen, collagen hydrolysate, hyaluronic acid and its salts, egg membrane, phytosterols, lysozyme chloride, sodium chondroitin sulfate, royal jelly, propolis, honey, glycyrrhizic acid, glycyrrhetinic acid, γ-linolenic acid, kojic acid and its esters, gibberellic acid, glycine, L-aspartic acid and its salts, L-cysteine ​​and its salts, DL-alanine, L-serine, DL-silyl... Amino acids, L-methionine, DL-methionine, L-lysine hydrochloride, L-threonine, inositol, maltol, pantothenate, D-panthenol, retinyl acetate, allantoin, pyridoxine hydrochloride, cortisone, nicotinamide, vitamins, estrogen, hematoxylin, uric acid, orotic acid, aloe-emodin, azelaic acid, rosin acid, usnic acid, laminarin, thymosin, alizarin, limonene, baicalin, deoxycholic acid, guaiac wood, hesperidin, tannic acid, selenium disulfide, tea saponins, triethyl citrate, butylated hydroxytoluene, phytic acid, polydimethyldiallylammonium chloride.

[0050] Experimental Example

[0051] The instruments, reagent kits, cells, and reagents used in the experiments were all available for purchase through publicly available commercial channels. These are commonly used instruments, reagent kits, cells, and reagents in the field. The operation procedures for the instruments and reagent kits were performed in accordance with their instruction manuals.

[0052] I. Main Instruments and Reagents

[0053] Table 1. Main instruments used in the experiment

[0054]

[0055]

[0056] Table 2. Main reagents used in the experiment

[0057]

[0058] Table 3 Primer sequences used in the experiment

[0059]

[0060] II. Test System

[0061] 1. Test materials

[0062] Ingredient 1: Acetyl hexapeptide-8, Trade name: Amplified peptide.

[0063] Raw material 2: N-acetylneuraminic acid, trade name: sialic acid, Wuhan Zhongke Guanggu Green Biotechnology Co., Ltd.

[0064] 2. Test cells

[0065] Cell type: HFF-1 human skin fibroblasts.

[0066] Source: Shanghai Cell Bank, Chinese Academy of Sciences Type Culture Collection Committee.

[0067] Cell type: adherent cells.

[0068] Culture conditions: 5% CO2, 37℃ constant temperature incubator.

[0069] Culture medium: 89% DMEM + 10% FBS + 1% antibiotics.

[0070] 3. Test methods

[0071] 3.1 Preparation of reagents

[0072] The test substance and control were prepared into a stock solution using DMSO or ddH2O, and then diluted to the appropriate concentration using serum-free culture medium or PBS.

[0073] Col-I antibody was diluted 1:100 with antibody dilution buffer, and Elastin antibody was diluted 1:50 with antibody dilution buffer. Both should be kept away from light and prepared fresh for immediate use.

[0074] 3.2 Experimental Grouping

[0075] The grouping and administration of N-acetylneuraminic acid and acetyl hexapeptide-8 synergistic effect test are shown in Table 4:

[0076] Table 4. Grouping and Dosing of the Synergistic Effect Test of N-acetylneuraminic Acid and Acetylhexapeptide-8

[0077]

[0078] (In combination therapy, for example, the specific regimen for administering N-acetylneuraminic acid and acetyl hexapeptide-8 at a total of 20 μg / ml and a mass ratio of 1:1 is: N-acetylneuraminic acid 10 μg / ml and acetyl hexapeptide-8 10 μg / ml in combination.)

[0079] 3.3 Operating Procedures

[0080] 3.3.1 qPCR detection

[0081] Cells in the exponential growth phase were used to prepare a single-cell suspension, and HFF-1 was added at a concentration of 1×10⁻⁶. 5Cells were seeded at a density of 10% fetal bovine serum (FBS) in 6-well plates and cultured at 37°C for 24 hours to allow cell adhesion, with a final culture volume of 2 mL. The supernatant was then discarded, and serum-free medium or freshly prepared serum-free medium containing different drug concentrations was added for incubation, with a final culture volume of 2 mL. After 48 hours, RNA was extracted from each well according to standard RNA extraction procedures, and the expression of Col-I, Col-IV, and Elastin mRNA was detected by qPCR.

[0082] Figure 1 This is a schematic diagram of the synergistic effect assay of N-acetylneuraminic acid and acetyl hexapeptide-8.

[0083] 4. Experimental Results

[0084] Each experimental group was run in triplicate. Results were entered using Excel and statistically processed using Prism (Version 7) software, and expressed as mean ± standard error. One-way ANOVA was performed on each data point, and p < 0.05 was considered statistically significant.

[0085] Figure 2 A comparative diagram showing the effects of administering N-acetylneuraminic acid and acetyl hexapeptide-8 alone and in combination at different ratios on Col-I mRNA expression in HFF-1 cells.

[0086] Figure 3 A comparative diagram showing the effects of N-acetylneuraminic acid and acetyl hexapeptide-8 alone and in combination at different ratios on Col-IV mRNA expression in HFF-1 cells.

[0087] Figure 4 A comparative diagram showing the effects of N-acetylneuraminic acid and acetyl hexapeptide-8 alone and in combination at different ratios on Elastin mRNA expression in HFF-1 cells.

[0088] Note: (1) Figures 2-4 The different ratios mentioned above refer to the mass ratios of N-acetylneuraminic acid and acetyl hexapeptide-8 being 1:5, 1:1, and 2:1.

[0089] (2) Figures 2-4 In the study, #, ##, and ### represent the significant improvement in efficacy of the combined administration of acetyl hexapeptide-8 compared to monotherapy, with #: p < 0.05; ##: p < 0.01; and ###: p < 0.001.

[0090] (3) Figures 2-4In the study, *, **, and *** represent the significant improvement in efficacy of the combined administration of N-acetylneuraminic acid compared to monotherapy, with *: p < 0.05; **: p < 0.01; and ***: p < 0.001.

[0091] The experimental results show that:

[0092] Compared with acetyl hexapeptide-8 alone, the combined administration of N-acetylneuraminic acid and acetyl hexapeptide-8 at a ratio of 1:5 did not significantly promote the expression of Col-I mRNA, Col-IV mRNA, and Elastin mRNA in HFF-1 cells.

[0093] Compared with acetyl hexapeptide-8 alone, the combined administration of N-acetylneuraminic acid and acetyl hexapeptide-8 at a ratio of 1:1 or 2:1 significantly promoted Col-I mRNA expression in HFF-1 cells.

[0094] Compared with acetyl hexapeptide-8 alone, the combined administration of N-acetylneuraminic acid and acetyl hexapeptide-8 at a ratio of 1:1 or 2:1 significantly promoted Col-IV mRNA expression in HFF-1 cells.

[0095] Compared with acetyl hexapeptide-8 alone, the combined administration of N-acetylneuraminic acid and acetyl hexapeptide-8 at a ratio of 1:1 or 2:1 significantly promoted the expression of Elastin mRNA in HFF-1 cells.

[0096] Col-I (type I collagen) is the most abundant type of collagen in the human body and the most plentiful protein in vertebrates, playing a crucial role in skin firmness and elasticity. Col-IV (type IV collagen) is an important protein connecting the epidermis and dermis; a significant increase in type IV collagen expression can effectively improve skin sagging. Elastin, an elastin, is one of the most important structural proteins in the skin, and increased expression of elastin can significantly combat skin aging. This invention clearly and explicitly demonstrates through comparative proportions and experimental examples that using N-acetylneuraminic acid and acetyl hexapeptide-8 in a mass ratio of 1:5 does not achieve a synergistic effect in increasing skin collagen and elastin. However, using N-acetylneuraminic acid and acetyl hexapeptide-8 in mass ratios of 1:1 and 2:1 shows a significant synergistic effect, significantly promoting the expression of Col-I mRNA, Col-IV mRNA, and elastin mRNA in HFF-1 cells. Therefore, it is reasonable to believe that the combination of N-acetylneuraminic acid and acetyl hexapeptide-8, with the dosage of N-acetylneuraminic acid reaching or exceeding that of acetyl hexapeptide-8, can achieve a synergistic effect in increasing skin collagen and elastin, significantly enhancing anti-aging efficacy. Consequently, the synergistic use of N-acetylneuraminic acid and acetyl hexapeptide-8 in cosmetics and / or skincare products, with the dosage of N-acetylneuraminic acid reaching or exceeding that of acetyl hexapeptide-8, can achieve more pronounced anti-aging effects on the skin with lower application rates, improving skin firmness and elasticity, and reducing wrinkles.

[0097] Those skilled in the art will understand that the above embodiments are specific examples of implementing the present invention, and in practical applications, various changes in form and detail may be made without departing from the spirit and scope of the present invention.

Claims

1. The application of N-acetylneuraminic acid, characterized in that, The N-acetylneuraminic acid synergistically enhances the efficacy of acetyl hexapeptide-8, wherein the mass ratio of N-acetylneuraminic acid to acetyl hexapeptide-8 is (1~4):

1. The N-acetylneuraminic acid, without therapeutic intent, synergistically enhances the efficacy of acetyl hexapeptide-8 in promoting the expression of collagen and elastin in the skin.

2. The application according to claim 1, characterized in that, The mass ratio of the N-acetylneuraminic acid to the acetyl hexapeptide-8 is (1~2):

1.

3. A polypeptide composition with synergistic effects, characterized in that, By weight, it contains: N-acetylneuraminic acid: 1-4 parts; Acetyl hexapeptide-8: 1 part; The N-acetylneuraminic acid synergistically enhances the efficacy of acetyl hexapeptide-8 in promoting the expression of skin collagen and elastin.

4. The polypeptide composition with synergistic effect according to claim 3, characterized in that, By weight, it contains: N-acetylneuraminic acid: 1-2 parts; Acetyl hexapeptide-8: 1 part.

5. The use of the polypeptide composition with synergistic effect as described in claim 3 or 4 in the preparation of cosmetics.

6. The application according to claim 5, characterized in that, The cosmetic is applied to the outer surface of the skin.

7. The application according to claim 5, characterized in that, The cosmetic product is selected from one or more of the following: eye cream, face cream, serum, and lotion.

8. The application according to claim 7, characterized in that, The essence mentioned is a facial mask essence.

9. The application according to claim 5, characterized in that, The polypeptide composition is dispersed as an additive in a matrix raw material, which is selected from one or more of oily raw materials, powdery raw materials, gel-like raw materials, and solvent raw materials.

10. The application according to claim 5, characterized in that, The cosmetics also include one or more of the following: surfactants, fragrances and flavorings, pigments, moisturizers, preservatives, antioxidants, ultraviolet absorbers, chelating agents, astringents, penetration enhancers, and nutritional additives.

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