High-temperature-resistant xylanase mutant and application thereof
By optimizing the amino acid sequence of xylanase through genetic engineering, a high-temperature resistant mutant was designed, which solved the problems of low yield and insufficient temperature resistance of xylanase in industrial applications, and achieved high enzyme activity under high temperature conditions, making it suitable for the feed industry.
CN117384888BActive Publication Date: 2026-07-07QINGDAO VLAND BIOTECH GRP CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- QINGDAO VLAND BIOTECH GRP CO LTD
- Filing Date
- 2022-07-12
- Publication Date
- 2026-07-07
Abstract
The present application relates to the technical field of genetic engineering and protein modification, and particularly relates to a high-temperature-resistant xylanase mutant and application thereof. The present application is based on wild-type xylanase PT, and provides a mutant containing at least one of the following mutation sites: T23S, S39D, T41N, S62Q, L64M, W116V, A139G, Y144F, T149F, N160E / V. Compared with the wild type, after being treated at 95 DEG C for 5 min, the enzyme activity residual rate of the xylanase single-point mutant is increased by 10.0%-25.6%, and the heat resistance is significantly enhanced. The xylanase mutant containing the L64M single-point mutation has the strongest heat resistance, and the enzyme activity residual rate is as high as 87.90%, which is beneficial to the wide application of the xylanase mutant in the feed field.
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