Skin soothing composition and skin care product

Abstract

The present invention belongs to the technical field of skin care, and discloses a skin soothing composition and a skin care product, wherein the skin soothing composition comprises the following components by weight percentage: 30-50% wheat germ extract, 20-30% bifid yeast fermentation product filtrate, 20-30% calendula extract, 1-10% allantoin, and 1-10% 4-tert-butyl cyclohexanol. The present invention discloses a skin soothing composition, wherein the calendula extract can inhibit the expression of NF-κB and IL-6, the allantoin can inhibit the expression of TNF-α and IL-6, the 4-tert-butyl cyclohexanol can inhibit the overexpression of TRPV1, the wheat germ extract can provide the required nutrients for the probiotics of the skin, and promote the growth of the probiotics, and the bifid yeast fermentation product filtrate can positively affect and regulate the overall microecological environment of the skin, increase the level of antimicrobial peptides, and inhibit the overgrowth and expression of harmful bacteria; through the compounding of the above five components, the skin condition is improved from three dimensions of the nervous system, the immune system and the microecological system, and the skin is helped to be soothed.

Description

Technical Field

[0001] The invention relates to the technical field of skin care, and in particular to a skin soothing composition and a skin care product. Background Art

[0002] The constantly changing external environment, such as air pollution, irritation caused by frequent use and replacement of cosmetics, unstable seasons, extremely cold and hot weather changes, personal psychological pressure and physiological factors, can all induce skin sensitivity problems. Sensitive skin may be accompanied by inflammatory reactions such as redness, fever, itching, stinging, and vasodilation when stimulated by the external environment.

[0003] Sensitive skin refers to skin that exhibits high reactivity and low tolerance, and is generally considered to be a metastable state of skin. In recent years, more and more people have been troubled by sensitive skin. Epidemiological studies have shown that the prevalence of sensitive skin in women is 60%-70%, and in men it is 50%-60%; soothing compositions can effectively help sensitive skin relieve discomfort and improve skin comfort.

[0004] Prior art 1: Chinese patent application 201910602114.6 discloses a skin care product for soothing and repairing sensitive skin, which includes the following components by mass percentage: ceramide complex 5-15%, skin conditioner 1-10%, plant-derived emollient 5-15%, low molecular weight hyaluronic acid 0.08-0.12%, betaine 1.2-3%, polyol moisturizer 7.8-28%, thickener 0.1-0.8%, emulsifier 0.5-2% and water 35%-72%;

[0005] The ceramide complex comprises the following components by mass percentage: 15-20% dipropylene glycol, 10-12% glycerol, 3.0-4.5% hydrogenated lecithin, 0.1-0.5% lysolecithin, 1-3% ceramide, 0.5-1% cholesterol, 0.05-0.1% chelating agent, 3-5% 1,2-pentanediol, and the remainder water, the sum of all components being 100%.

[0006] The technical solution disclosed in the above patent application is capable of promoting the repair of skin barrier function and improving the skin's water retention capacity, so as to strengthen the barrier function, reduce the skin's reactivity to environmental factors, improve the skin's resistance to external stimuli, enhance the moisturizing of the epidermis, relieve skin discomfort, and improve the comfort of sensitive and reactive skin. It is suitable for long-term use by people with sensitive skin.

[0007] Similarly, prior art 2: Chinese patent application 202210749953.2 discloses a skin sensitive repair composition and its application, which relates to the field of cosmetic technology. The skin sensitive repair composition comprises the following weight percentage components: 0.25-2.4% of Fucus vesiculosus extract, 0.25-1.2% of rambutan peel extract, 0.2-1% of Mirabilis jalapa extract, and 0.2-1% of Centella asiatica leaf extract. The weight ratio of the Fucus vesiculosus extract to the rambutan peel extract is 1:(0.5-2). The weight ratio of the Mirabilis jalapa extract to the Centella asiatica leaf extract is 1:(0.5-2).

[0008] The technical solutions disclosed in the above patent applications provide a technical solution for skin sensitivity repair for people with sensitive skin from a more comprehensive perspective, starting from the mechanisms of improving skin barrier function, inhibiting skin nerve growth factor, and reducing the release of acute inflammatory factors. Through the screening of components and the optimization of proportions, a composition with a more complete sensitive repair mechanism and synergistic enhancement between components is proposed, achieving a sensitive repair effect of 1+1+1>3.

[0009] The above-mentioned prior art 1 and prior art 2 both have certain effects on soothing the skin, but the above two technical solutions only carry out comprehensive skin repair from the aspects of repairing the skin barrier function and soothing the skin, and do not deeply soothe the discomfort caused by sensitive skin. For some users who already have sensitive skin, the targeting is not strong enough and the effect of improving skin discomfort in a short period of time is not obvious enough.

[0010] Therefore, it is necessary to develop a composition that can deeply soothe the skin for sensitive skin to improve the skin soothing effect. Summary of the invention

[0011] One of the purposes of the present invention is to provide a skin soothing composition to solve the problem that the prior art only combines comprehensive skin care with repairing the skin barrier and soothing the skin, but does not deeply and efficiently soothe sensitive skin. The skin soothing composition has a significant skin soothing effect and comprehensively helps soothe the skin from multiple dimensions.

[0012] Another object of the present invention is to provide a skin care product containing the skin soothing composition of the present invention, which improves the skin condition from three dimensions of the nervous system, immune system and microecological system and helps soothe the skin.

[0013] To achieve the above object, the present invention provides a skin soothing composition, which comprises the following components by weight percentage: 30-50% wheat germ extract, 20-30% bifida fermentation product filtrate, 20-30% calendula extract, 1-10% allantoin, and 1-10% 4-tert-butyl cyclohexanol.

[0014] Preferably, the usage ratio of allantoin and 4-tert-butyl cyclohexanol is 1:1.

[0015] Preferably, the composition comprises the following components by weight percentage: 30-50% wheat germ extract, 20-30% bifida yeast fermentation product filtrate, 20-30% marigold extract, 5% allantoin, and 5% 4-tert-butyl cyclohexanol.

[0016] The present invention also provides a skin care product, comprising the above-mentioned skin soothing composition, wherein the content of the skin soothing composition in the skin care product is ≥1%.

[0017] Preferably, the content of the skin soothing composition in the skin care product is 1-3%.

[0018] Furthermore, the content of the skin soothing composition in the skin care product is preferably 1%, 1.5%, 2%, 2.5%, or 3%.

[0019] 4-tert-Butyl cyclohexanol can inhibit the overexpression of TRPV1, help enhance the skin tolerance threshold through regulatory effects, and significantly reduce burning and stinging sensations.

[0020] Wheat germ extract is rich in carbohydrate nutrients, such as α-glucan, mannose, rhamnose, fructan and galactan, B vitamins including niacinamide, pantothenic acid, thiamine, pyridoxine, riboflavin and folic acid, various amino acids and polypeptide small molecule components, as well as flavonoids mainly composed of tricholoma and polyphenols mainly composed of ferulic acid and chlorogenic acid. These ingredients can fully provide the necessary nutrients for the probiotics of the skin.

[0021] The filtrate of bifid yeast fermentation product can positively influence and regulate the microecology, helping the skin restore to a normal bacterial balance.

[0022] Beneficial Effects

[0023] Compared with the prior art, the present invention has at least the following advantages:

[0024] (1) The present invention discloses a skin soothing composition, wherein the calendula extract can inhibit the expression of NF-κB and IL-6, allantoin can inhibit the expression of TNF-α and IL-6, 4-tert-butyl cyclohexanol can inhibit the overexpression of TRPV1, the wheat germ extract can provide the necessary nutrients for the probiotics of the skin and promote the growth of the probiotics, and the filtrate of the fermentation product of bifid yeast can positively affect and regulate the overall microecological environment of the skin, increase the level of antimicrobial peptides, and inhibit the overgrowth and expression of harmful bacteria; through the compounding of the above five components, the skin condition is improved from three dimensions of the nervous system, the immune system and the microecological system, and the skin is soothed;

[0025] (2) The present invention optimizes compounding by compounding the above five raw materials within a certain range, with wheat germ extract, bifid yeast fermentation product filtrate and marigold extract as the main ingredients, and compounding allantoin and 4-tert-butyl cyclohexanol to produce a synergistic effect, which can significantly improve the soothing effect on the skin;

[0026] (3) The present invention discloses a skin care product comprising the skin soothing composition of the present invention. The content of the skin soothing composition within a certain range can exert excellent skin soothing effects and control production costs to a certain extent. DETAILED DESCRIPTION

[0027] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0028] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0029] In the following examples and comparative examples, wheat germ extract, bifid yeast fermentation product filtrate, yeast fermentation product filtrate, honeysuckle extract and calendula extract were purchased from Guangzhou Ganeng Biotechnology Co., Ltd., allantoin was purchased from DSM (China) Co., Ltd., rice husk extract was purchased from Jiangxi Bajiu New Material Technology Co., Ltd., 4-tert-butyl cyclohexanol and bisabolol were purchased from Symrise (Shanghai) Co., Ltd., and betaine was purchased from Wuhan Optics Valley Bioengineering Co., Ltd.

[0030] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0031] In the following examples and comparative examples, unless otherwise specified, the parts are parts by weight and the % are percentages by weight.

[0032] Example 1

[0033] A skin soothing composition is obtained by uniformly mixing 50 parts of wheat germ extract, 5 parts of allantoin, 5 parts of 4-tert-butyl cyclohexanol, 20 parts of marigold extract and 20 parts of bifid yeast fermentation product filtrate.

[0034] Example 2

[0035] A skin soothing composition is obtained by uniformly mixing 30 parts of wheat germ extract, 5 parts of allantoin, 5 parts of 4-tert-butyl cyclohexanol, 30 parts of marigold extract and 30 parts of bifid yeast fermentation product filtrate.

[0036] Example 3

[0037] A skin soothing composition is obtained by uniformly mixing 45 parts of wheat germ extract, 1 part of allantoin, 1 part of 4-tert-butyl cyclohexanol, 25 parts of marigold extract and 28 parts of bifid yeast fermentation product filtrate.

[0038] Example 4

[0039] A skin soothing composition is obtained by uniformly mixing 40 parts of wheat germ extract, 10 parts of allantoin, 10 parts of 4-tert-butyl cyclohexanol, 20 parts of marigold extract and 20 parts of bifid yeast fermentation product filtrate.

[0040] Example 5

[0041] A skin soothing composition is obtained by uniformly mixing 50 parts of wheat germ extract, 8 parts of allantoin, 2 parts of 4-tert-butyl cyclohexanol, 20 parts of marigold extract and 20 parts of bifid yeast fermentation product filtrate.

[0042] Comparative Example 1

[0043] A composition is obtained by uniformly mixing 50 parts of wheat germ extract, 20 parts of marigold extract, 10 parts of allantoin and 20 parts of bifid yeast fermentation product filtrate.

[0044] Comparative Example 2

[0045] A composition is obtained by uniformly mixing 50 parts of wheat germ extract, 20 parts of marigold extract, 10 parts of 4-tert-butyl cyclohexanol and 20 parts of bifid yeast fermentation product filtrate.

[0046] Comparative Example 3

[0047] A composition is obtained by uniformly mixing 62.5 parts of wheat germ extract, 6.25 parts of allantoin, 25 parts of marigold extract and 6.25 parts of 4-tert-butyl cyclohexanol.

[0048] Comparative Example 4

[0049] A composition is obtained by uniformly mixing 62.5 parts of wheat germ extract, 6.25 parts of allantoin, 25 parts of bifid yeast fermentation product filtrate and 6.25 parts of 4-tert-butyl cyclohexanol.

[0050] Comparative Example 5

[0051] A composition is obtained by uniformly mixing 10 parts of allantoin, 10 parts of 4-tert-butyl cyclohexanol, 40 parts of marigold extract and 40 parts of bifid yeast fermentation product filtrate.

[0052] Comparative Example 6

[0053] A composition is obtained by uniformly mixing 50 parts of wheat germ extract, 5 parts of allantoin, 5 parts of bisabolol, 20 parts of marigold extract and 20 parts of bifid yeast fermentation product filtrate.

[0054] Comparative Example 7

[0055] A composition is obtained by uniformly mixing 50 parts of rice husk extract, 5 parts of allantoin, 5 parts of 4-tert-butyl cyclohexanol, 20 parts of marigold extract and 20 parts of bifid yeast fermentation product filtrate.

[0056] Comparative Example 8

[0057] A composition is obtained by uniformly mixing 50 parts of wheat germ extract, 5 parts of betaine, 5 parts of 4-tert-butyl cyclohexanol, 20 parts of marigold extract and 20 parts of bifid yeast fermentation product filtrate.

[0058] Comparative Example 9

[0059] A composition is obtained by uniformly mixing 50 parts of wheat germ extract, 5 parts of allantoin, 5 parts of 4-tert-butyl cyclohexanol, 20 parts of honeysuckle extract and 20 parts of bifid yeast fermentation product filtrate.

[0060] Comparative Example 10

[0061] A composition is prepared by uniformly mixing 50 parts of wheat germ extract, 5 parts of allantoin, 5 parts of 4-tert-butyl cyclohexanol, 20 parts of marigold extract and 20 parts of yeast fermentation product filtrate.

[0062] Performance Testing

[0063] 1. Determination of hyaluronidase inhibition rate

[0064] Experimental Principle

[0065] Hyaluronidase is a specific cleavage enzyme of hyaluronic acid, a participant in allergic reactions, and has a strong correlation with the release of histamine by mast cells. Whether the test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0066] The experimental reference compares the hyaluronidase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain soothing effect.

[0067] Experimental setup

[0068] Composition test: dilute the sample with pure water to a solution with a volume fraction of 10%;

[0069] Negative control: pure water.

[0070] Experimental methods

[0071] Set up a sample group, a sample background group, a solvent group and a solvent background group. Each group needs to have 3 parallels. Add different reagent solutions to the four groups respectively, shake well, and leave at room temperature for 30 minutes for color development. Use a UV spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0072] Calculation formula

[0073] Hyaluronidase inhibition rate % = [1-(CD) / (AB)] × 100%

[0074] Where: A is the absorbance of the reaction solution without sample; B is the absorbance of the reaction solution without sample and enzyme;

[0075] C is the absorbance of the reaction solution containing the sample and the enzyme; D is the absorbance of the reaction solution containing the sample but not the enzyme.

[0076] Data analysis

[0077] The statistical analysis software was SPSS, and the independent sample t-test was used for comparison between the test samples and the negative control. The above statistical analyses were all two-tailed tests, with a significance level of α=0.05; P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0078] The compositions prepared in Examples 1-5 and Comparative Examples 1-10 were tested according to the above experiment, and the results are shown in Table 1;

[0079] Table 1 Hyaluronidase inhibition rate test results of the compositions prepared in Examples 1-5 and Comparative Examples 1-10

[0080]

[0081]

[0082] According to the results in Table 1, we can see that:

[0083] According to the data of Examples 1-5, the technical solution of the present invention using wheat germ extract, calendula extract, allantoin, 4-tert-butyl cyclohexanol, and bifid yeast fermentation product filtrate has obvious soothing effect; among them, the comparison of the data of Example 5 and Example 1 shows that the dosage ratio of allantoin and 4-tert-butyl cyclohexanol has a more obvious effect on the skin soothing effect. When the dosage ratio of allantoin and 4-tert-butyl cyclohexanol is 1:1, the maximum synergistic soothing effect can be exerted.

[0084] According to the data comparison of Example 1 and Comparative Examples 1-5, the present invention adopts wheat germ extract, calendula extract, allantoin, 4-tert-butyl cyclohexanol, and bifid yeast fermentation product filtrate to produce a synergistic effect of improving the skin soothing effect; among them, the hyaluronidase inhibition rate data of Comparative Examples 1, 3 and 5 are good, and the P value is less than 0.05, indicating that it has a certain hyaluronidase inhibition effect and skin soothing effect, while the hyaluronidase inhibition rate data of Comparative Examples 2 and 4 are lower, from which it can be inferred that when calendula extract and allantoin are compounded, they can It can produce a certain hyaluronidase inhibition and skin soothing effect; and the data of Example 1 show that adding wheat germ extract, 4-tert-butyl cyclohexanol and bifid yeast fermentation product filtrate on the basis of the compound of calendula extract and allantoin can produce a synergistic effect of enhancing the skin soothing effect of calendula extract and allantoin, and according to the data of Comparative Examples 1-5, it can be seen that in the synergistically enhanced composition for improving skin soothing effect produced by the present invention, wheat germ extract, calendula extract, allantoin, 4-tert-butyl cyclohexanol and bifid yeast fermentation product filtrate are indispensable.

[0085] According to the data comparison of Example 1 and Comparative Examples 6-10, the present invention adopts the wheat germ extract, calendula extract, allantoin, 4-tert-butyl cyclohexanol, and the filtrate of the bifid yeast fermentation product to produce a synergistic effect of improving the skin soothing effect; wherein Comparative Examples 6 and 10 have certain hyaluronidase inhibition and skin soothing effects, and the reason is the same as that of Comparative Examples 1, 3 and 5, because when the calendula extract and allantoin are compounded, they can produce certain hyaluronidase inhibition and skin soothing effects;

[0086] The hyaluronidase inhibition and skin soothing effects shown in Comparative Example 6 are further improved, and the possible reason is that bisabolol further improves the hyaluronidase inhibition and skin soothing effects of the combination of calendula extract and allantoin, but bisabolol does not synergistically enhance the skin soothing effects of calendula extract and allantoin with wheat germ extract and bifid yeast fermentation product filtrate, so its enhancement effect is significantly weaker than the technical solution of the present invention;

[0087] Among them, Comparative Example 7 also uses a combination of calendula extract and allantoin, which theoretically should be able to produce a certain hyaluronidase inhibition effect and skin soothing effect, but the effect it shows is greatly reduced. It may be that the combination of other components with calendula extract and allantoin has a negative effect, resulting in a significant decrease in the skin soothing effect of the composition.

[0088] However, the technical solutions of replacing allantoin with betaine and replacing Calendula officinalis extract with honeysuckle extract in Comparative Examples 8 and 9 did not produce a good effect of improving the skin soothing effect.

[0089] 2. Human soothing effect test

[0090] Human soothing test method

[0091] 35 subjects were screened for the human soothing efficacy test of the essence containing different concentrations of the composition of Example 1, and 3 test areas were marked on both sides of the arm of each subject, for a total of 6 test areas per person; 70 subjects were screened for the human soothing efficacy test of the essence containing 2% of the composition of Examples 1-5 and Comparative Examples 1-10, and 4 test areas were marked on both sides of the arm of each subject, for a total of 8 test areas per person.

[0092] Subject exclusion criteria:

[0093] 1) Have ever had severe skin disease symptoms due to the use of cosmetics

[0094] 2) Use of topical steroids, tacrolimus hydrate ointment, or nonsteroidal anti-inflammatory topical agents on the test site within 4 weeks before the start of the trial

[0095] 3) Suffering from comorbidities that may affect the evaluation of this trial

[0096] 4) Others that are deemed inappropriate by the responsible physician and the physician who shared the responsibility for the trial

[0097] Before using the sample (0h): the subjects cleaned the inside of both arms and sat quietly at the required temperature for 20 minutes. During this period, they were not allowed to walk or eat. The test site was exposed and they remained relaxed. The test area was marked. The area of ​​the test area was at least 3cm*3cm, and the interval was at least 1cm. The sample area and the control area were randomly allocated according to the blind table. The skin barrier of the test area and the control area was damaged by tearing with low-allergenic tape. The staff used red pigment ( After the MX18) probe was used to measure the basic value of the test area of ​​the subject, the staff applied a single even amount of (2.0±0.1) mg / cm2; the blank control used a cold compress bag for continuous cold compress relief.

[0098] After using the sample (0.5h, 1h, 2h): The same laboratory technician will measure the red pigment in the test area of ​​the subject and record the data.

[0099] Instruments and equipment and evaluation instructions

[0100] Table 2 Test instrument information

[0101]

[0102] Test Results

[0103] SPSS software was used to perform descriptive statistics on each test value. The Shapiro-Wilk Test was used to test the significance of the normal distribution of the data change value. If P>0.05, it was normally distributed, and the paired sample t test was performed, with a significant difference level of α=0.05. If P<0.05, it was non-normally distributed, and the Wilcoxon signed rank test was performed.

[0104] 2.1 Moisturizing efficacy test of serums containing different concentrations of compositions

[0105] According to Table 3, essences containing different concentrations of the composition of Example 1 were prepared, and the amount of each substance was expressed by mass fraction;

[0106] Table 3 Analysis of human soothing test sample formulas

[0107] Composition Blank control Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Deionized water To 100 To 100 To 100 To 100 To 100 To 100 Example 1 0 0.5 1.0 2.0 3.0 4.0 Reduction glue 0.25 0.25 0.25 0.25 0.25 0.25 preservative Moderate Moderate Moderate Moderate Moderate Moderate total 100 100 100 100 100 100

[0108] The essence prepared in Table 3 was tested according to the above human soothing test method, and the results are shown in Table 4;

[0109] Table 4 Test results of the soothing effect of the essence containing different concentrations of the composition of Example 1 on human body

[0110]

[0111]

[0112] According to the data of each sample in Table 4 and the comparison with the control group, the results are shown in Table 5;

[0113] Table 5 Comparative analysis of red pigment test results at different concentrations in Example 1 and the control group

[0114]

[0115] According to the results in Table 4 and Table 5, we can see that:

[0116] According to the test results in Table 4, each sample will gradually alleviate skin redness over time. For the skin redness caused by tearing with a hypoallergenic tape, after using samples 1-5 containing the composition of Example 1, the red pigment in each concentration gradient will significantly decrease from 30 minutes, while the red pigment in the blank control will significantly decrease from 1 hour, indicating that the skin redness caused by physical tearing will slowly subside on its own over time, but when using samples 1-5 containing the composition of Example 1, the red pigment decreases faster.

[0117] According to the data analysis results in Table 5, by comparing the blank control and the sample change rates of each concentration gradient, it can be seen that: the skin red pigment change rate of sample 1 in the three time periods of T0 (0h)-T3 (3h) relative to the change rate of the blank control, there is no significant difference between the two groups; for sample 2 during T0 (0h)-T3 (3h), the skin red pigment change rate in the three time periods relative to the change rate of the blank control, there is no significant difference between the two groups in the change rate of T0 (0h)-T1 (30min) compared with the blank control, but there is a significant difference between the two groups in the change rate of T1 (30min)-T3 (3h) compared with the blank control; the skin red pigment change rate of samples 3, sample 4 and sample 5 in the three time periods of T0 (0h)-T3 (3h) is significantly different from that of the blank control. There are significant differences between the two groups in the rate of change of skin red pigment relative to the rate of change of the blank control; this indicates that compared with the rate of change of red pigment in the blank control, the composition containing 0.5% of Example 1 of the present invention does not show obvious skin soothing effect, and shows significant skin soothing effect starting from a concentration of 1.0% of the composition of Example 1 of the present invention, and as the content of the composition of Example 1 of the present invention increases from 1.0% to 3.0%, the soothing effect becomes more significant, among which the effect of the content of 2.0-3.0% is more excellent than that of 1.0%, so 2.0-3.0% concentration is more preferred for the composition, and although the composition with a concentration greater than 3.0% has a significant soothing effect, the increase in concentration is of little significance, and on the contrary will increase the cost of the product.

[0118] 2.2 Human soothing efficacy test of different compositions

[0119] The essences of Examples 1-5 and Comparative Examples 1-10 were prepared according to the formula of Sample 3 in Table 3, and the compositions of Examples 1-5 and Comparative Examples 1-10 replaced Example 1 to obtain essences containing 2% of the compositions of Examples 1-5 and Comparative Examples 1-10, respectively, and then tested according to the above human soothing test method. The results are shown in Table 6;

[0120] Table 6 Test results of the soothing effect of the essence containing 2% of the composition of Examples 1-5 and Comparative Examples 1-10 on human body

[0121]

[0122]

[0123]

[0124] According to the data of each embodiment 1-5 and comparative examples 1-10 in Table 6 and the comparison with the control group, the results are shown in Table 7;

[0125] Table 7 Analysis of the test results of the red pigment of the 2% composition essence

[0126]

[0127]

[0128]

[0129] According to the results in Table 6 and Table 7:

[0130] According to the data in Table 6, as time goes by, the compositions of Examples 1-5 and Comparative Examples 1-10 and the blank control all show a certain degree of relief of skin redness. Regarding the skin redness caused by tearing with a low-allergenic tape, it can be seen from the comparison between Examples 1-5 and the blank control that the technical solution of the present invention can accelerate the decline rate of red pigment in a short period of time, showing a significant skin soothing effect; among them, according to the comparison between Example 1 and Example 5, in terms of the soothing effect on the human body, the maximum synergistic soothing effect can be exerted when the usage ratio of cystin and 4-tert-butyl cyclohexanol is 1:1.

[0131] According to the data in Table 6, among Comparative Examples 1-10, the performance of Comparative Examples 6, 7, 9 and 10 in the whole process of alleviating skin redness is basically the same as that of the blank control group, indicating that the skin soothing effect of the technical solution of the present invention using wheat germ extract, calendula extract, allantoin, 4-tert-butyl cyclohexanol and bifid yeast fermentation product filtrate is not a simple superposition of the efficacy of the components, but a significant synergistic effect between the components; replacing any component in the technical solution of the present invention with other components with similar efficacy is likely to greatly reduce the human skin soothing effect of the present invention.

[0132] According to the data in Table 7, by comparing the change rates of the blank control and each composition, it can be seen that: the skin red pigment change rates of Comparative Examples 1-3 and Comparative Examples 5-10 in the three time periods of T0 (0h) -T3 (3h) relative to the change rate of the blank control, there is no significant difference between the two groups; Comparative Example 4 in the three time periods of T0 (0h) -T3 (3h), the change rate of the skin red pigment relative to the change rate of the blank control, T0 (0h) -T1 (30min) has a significant difference between the two groups compared with the blank control, but T1 (30min) -T3 (3h) has no significant difference between the two groups compared with the blank control; Examples 1-5 in the three time periods of T0 (0h) -T3 (3h) relative to the change rate of the blank control, there are significant differences between the two groups; Compared with the red pigment change rate of the blank control, Examples 1-5 have a significant soothing effect, while The soothing effect of Examples 1-10 on the skin is not so obvious. Among them, the change rate of Comparative Example 4 during T0 (0h)-T1 (30min) has a significant difference between the two groups compared with the blank control. It is speculated that allantoin inhibits the expression of the most core inflammatory factors TNF-α and IL-6. While 4-tert-butyl cyclohexanol inhibits the overexpression of TRPV1, it can also significantly help relieve skin redness in a short time. Wheat germ extract can provide the required nutrients for the probiotics of the skin and promote the growth of probiotics. The filtrate of the fermentation product of bifid yeast helps regulate the overall microecological environment of the skin, and works together with the wheat germ extract. It also helps soothe the skin from three dimensions of the nervous system, immune system and microecological system, so it also has significant differences. Among them, the significant difference shown in the short period of time T1 (30min) is the most obvious. As time goes by, the difference in skin soothing efficacy gradually decreases. Compared with Example 1, the effect difference is relatively large.

[0133] Based on this, a careful comparison of the data of Example 1, Comparative Example 4 and the blank control group shows that Comparative Example 4 significantly increases the soothing effect on human skin compared with the blank control group, and the change rate growth values ​​are T1 (30min) -8.65%, T2 (1h) -7.25%, and T3 (2h) -6.13% respectively; and the soothing effect on human skin improved in Example 1 compared with the blank control group is more significant, more stable and long-lasting, and the change rate growth values ​​are T1 (30min) -12.33%, T2 (1h) -11.99%, and T3 (2h) -14.64% respectively. The synergistic effect of improving the skin soothing effect produced by the compounding of wheat germ extract, calendula extract, allantoin, 4-tert-butyl cyclohexanol, and bifid yeast fermentation product filtrate in Example 1 is more significant, more stable and long-lasting than that in Comparative Example 1.

[0134] Based on the above information, it is speculated that the mechanism of action of the composite composition of the present invention is as follows: calendula extract can inhibit the expression of NF-κB and IL-6, allantoin can inhibit the expression of TNF-α and IL-6, 4-tert-butyl cyclohexanol can inhibit the overexpression of TRPV1, wheat germ extract can provide the required nutrients for the probiotics of the skin and promote the growth of probiotics, and the filtrate of the fermentation product of bifid yeast can positively affect and regulate the overall microecological environment of the skin, increase the level of antimicrobial peptides, and inhibit the excessive growth and expression of harmful bacteria; through the compounding of these five ingredients, the skin condition can be improved from three dimensions of the nervous system, immune system and microecological system, and help soothe the skin.

[0135] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A skin soothing composition, characterized in that: The invention is composed of the following components by weight percentage: 30-50% wheat germ extract, 20-30% bifida yeast fermentation product filtrate, 20-30% marigold extract, 1-10% allantoin and 1-10% 4-tert-butyl cyclohexanol, wherein the dosage of allantoin and 4-tert-butyl cyclohexanol is 1:

1.

2. A skin care product, characterized in that: The skin-soothing composition according to claim 1 is included, wherein the content of the skin-soothing composition in the skin care product is ≥ 1%.

3. The skin care product according to claim 2, characterized in that: The content of the skin soothing composition in the skin care product is 1-3%.

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