A traditional Chinese medicine compound for liver protection in aquaculture animals, its preparation method and application

Abstract

This invention discloses a traditional Chinese medicine compound for liver protection in aquatic animals, its preparation method, and its application, belonging to the field of liver protection and prevention in aquatic animals. The compound comprises the following ingredients: rhubarb, dried tangerine peel, perilla, artemisia capillaris, *Hedyotis diffusa*, forsythia, poria cocos, codonopsis, and licorice. The method of using this compound is to mix it with feed during the rapid growth period of aquatic animals, administering it for 3-5 consecutive days, followed by a 2-3 day break, constituting one course of treatment. It is used to treat symptoms such as liver enlargement, atrophy, and abnormal color caused by spoiled feed and certain bacterial infections. The medicine is safe to use, leaves no harmful residues, and meets national standards for pollution-free food safety.

Description

Technical Field

[0001] This invention belongs to the field of liver protection and prevention in aquatic animals, specifically relating to a traditional Chinese medicine compound for liver protection in aquatic animals, its preparation method, and its application. Background Technology

[0002] my country is a major aquaculture country, and in recent years it has developed rapidly in an intensive manner. However, the excessive pursuit of yield and growth rate has led to excessive or unbalanced feed nutrients, abuse of drugs, use of inferior and unconventional raw materials, and ultimately caused a series of aquatic animal diseases, among which liver damage and diseases that induce liver disease are the most harmful.

[0003] The liver is a vital metabolic and detoxification organ for aquatic animals, and it also plays a role in hematopoiesis. Liver dysfunction affects nutrient absorption and metabolism, consequently impacting aquatic animal growth, leading to weakened body fat, decreased immunity, and significant economic losses in aquaculture. Fish also produce and export enzymes from the liver; fibrinogen and thrombin are essential functional substances of the liver. When fish develop liver disease, blood vessels are prone to rupture, and blood clotting function is impaired. Under stress from other conditions, they may experience severe stress syndrome, even leading to mass mortality.

[0004] Prevention and treatment of liver injury in fish mainly involve balancing nutrients in the feed, using anti-lipid factors to strengthen liver function, or using large doses of antibiotics. While anti-lipid factors play a good supporting role in reducing liver fat, their effect on liver damage and the repair of damaged cells is minimal. Antibiotic treatment, although bringing good results and significant economic benefits, also disrupts the aquatic animal's microbial flora, promotes the increase of drug-resistant strains, and leads to antibiotic residues in the body. Therefore, developing an effective alternative without these side effects is crucial.

[0005] Traditional Chinese medicine has the characteristics of no drug resistance, no residue, no pollution and no drug-induced diseases. Domestic and foreign studies have shown that traditional Chinese medicine has a significant effect on the prevention and treatment of liver diseases in aquatic animals. Developing traditional Chinese medicine suitable for nourishing and protecting the liver of aquatic animals is of great significance to the aquaculture industry. Summary of the Invention

[0006] To overcome the shortcomings and deficiencies of existing technologies, the present invention aims to provide a traditional Chinese medicine compound for liver protection in aquaculture animals, its preparation method, and its application. This traditional Chinese medicine compound can effectively repair liver damage, promote growth performance, and enhance resistance to diseases.

[0007] The objective of this invention is achieved through the following technical solution:

[0008] A traditional Chinese medicine compound for protecting the liver of aquatic animals includes the following ingredients in parts by weight: rhubarb 16±2 parts, dried tangerine peel 18±2 parts, perilla 18±2 parts, artemisia capillaris 30±2 parts, scutellaria barbata 30±2 parts, forsythia suspensa 20±2 parts, poria cocos 18±2 parts, codonopsis pilosula 16±2 parts, and licorice 20±10 parts.

[0009] A further preferred embodiment of the traditional Chinese medicine compound for protecting the liver of aquatic animals comprises the following ingredients in parts by weight: rhubarb 16±1 parts, dried tangerine peel 18±1 parts, perilla 18±1 parts, artemisia capillaris 30±1 parts, scutellaria barbata 30±1 parts, forsythia suspensa 20±1 parts, poria cocos 18±1 parts, codonopsis pilosula 16±1 parts, and licorice 20±10 parts.

[0010] The preparation method of the above-mentioned traditional Chinese medicine compound for protecting and nourishing the liver in aquatic animals includes the following steps:

[0011] Take rhubarb, dried tangerine peel, perilla, artemisia capillaris, scutellaria barbata, forsythia, poria cocos, codonopsis pilosula, and licorice, dry them, pulverize them, sieve them, and mix them evenly to obtain a traditional Chinese medicine composition for protecting the liver.

[0012] Preferably, the drying temperature is 60-70°C; more preferably 60°C.

[0013] Preferably, the sieving is performed through a 60-100 mesh sieve; more preferably, through an 80 mesh sieve.

[0014] The application of the aforementioned traditional Chinese medicine compound in the preparation of feed for liver protection in aquatic animals.

[0015] Preferably, the aquatic animals are aquatic animals in their rapid growth phase;

[0016] Preferably, the aquatic animals include fish, etc.

[0017] More preferably, the aquatic farmed animals include at least one of California bass and mandarin fish.

[0018] Preferably, the amount of the traditional Chinese medicine compound added to the feed is 2±1g / kg.

[0019] Preferably, the method of using the traditional Chinese medicine compound is to mix it with feed and feed it to aquatic animals during their rapid growth period, use it for 3 to 5 days and stop for 2 to 3 days as one course of treatment, and use it for 3 to 5 courses of treatment.

[0020] More preferably, the method of using the traditional Chinese medicine compound is to mix it with feed and feed it to aquatic animals during their rapid growth period, use it for 3 to 5 days and stop for 2 to 3 days as one course of treatment, and use it for 3 courses of treatment.

[0021] More preferably, the method of using the traditional Chinese medicine compound is to mix it with feed and feed it to aquatic animals during their rapid growth period, use it for 3 consecutive days and stop for 2 days as one course of treatment, and use it for 3 consecutive courses of treatment.

[0022] The present invention has the following advantages and effects compared with the prior art:

[0023] This invention uses rhubarb, dried tangerine peel, and codonopsis as the principal herbs, perilla, artemisia capillaris, and scutellaria barbata as the assistant herbs, forsythia and poria cocos as adjuvant herbs, and licorice as the guiding herb. The method of using this traditional Chinese medicine compound is to mix it with feed and feed it to aquatic animals during their rapid growth period, for 3-5 consecutive days, followed by a 2-3 day break, constituting one course of treatment. It is used to treat symptoms such as liver enlargement, atrophy, and abnormal color caused by spoiled feed and certain bacterial infections. The medicine is safe to use, leaves no harmful residues, and meets national pollution-free food safety standards. Attached Figure Description

[0024] Figure 1 These are anatomical diagrams of the liver in Examples 1 to 6; where A: Example 1, B: Example 2, C: Example 3, D: Example 4, E: Example 5, and F: Example 6.

[0025] Figure 2 These are anatomical diagrams of the liver in Examples 7 to 12; where a: Example 7, b: Example 8, c: Example 9, d: Example 10, e: Example 11, f: Example 12.

[0026] Figure 3 These are anatomical diagrams of the liver, shown in scales 1 and 2. Detailed Implementation

[0027] The present invention will now be described in further detail with reference to the embodiments and accompanying drawings, but the embodiments of the present invention are not limited thereto. To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention; that is, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0028] Examples 1-6

[0029] The traditional Chinese medicine compound used for liver protection in aquaculture animals includes the following ingredients in the indicated weight proportions: See Table 1 for details:

[0030] Table 1

[0031] Example 1 2 3 4 5 6 rhubarb 16 15 17 16 15 17 Dried tangerine peel 18 17 19 18 17 19 Perilla 18 18 19 17 19 17 Artemisia capillaris 30 30 29 31 30 29 Creek Yellow Grass 30 30 29 30 29 31 Forsythia 20 20 21 19 21 19 Poria 18 18 17 19 18 17 Codonopsis pilosula 16 16 15 16 15 17 licorice 30 30 25 20 15 10

[0032] The preparation method of a traditional Chinese medicine compound for liver protection in aquaculture animals includes the following steps:

[0033] The raw materials of rhubarb, dried tangerine peel, perilla, artemisia capillaris, scutellaria barbata, forsythia, poria cocos, codonopsis pilosula, and licorice are dried at 60℃, pulverized and passed through an 80-mesh sieve, and mixed evenly to obtain a traditional Chinese medicine compound for protecting the liver.

[0034] Effective Examples 1-12 and Comparative Examples 1-2

[0035] The Chinese herbal compound added in Examples 1-6 are the same as those in Examples 1-6, and the target species is California bass; the Chinese herbal compound added in Examples 7-12 are the same as those in Examples 1-6, and the target species is mandarin fish; Comparative Example 1 is California bass, without the addition of Chinese herbal compound; Comparative Example 2 is mandarin fish, without the addition of Chinese herbal compound.

[0036] Efficacy testing and research on liver damage repair in California bass and mandarin fish.

[0037] 1. Background restoration and methods for California bass and mandarin fish

[0038] The Hetangyuese Aquaculture Farm in Jiangou Village, Haiou Island, Shilou Town, Panyu District, Guangzhou City, has a total area of ​​205 mu (approximately 13 hectares), with ponds ranging from 4 to 5 mu (approximately 0.67-0.33 hectares) in size and a water depth of 2.5 meters. On August 17, 2023, largemouth bass showed swollen livers with a yellowish tinge, underdeveloped stomachs, and slow feeding, resulting in 15-20 deaths per day. On August 21, 2023, feed-fed mandarin fish were found to have swollen livers, white livers, empty stomachs, and slow feeding, with 10-20 deaths per day. Two ponds were selected as experimental ponds: one for largemouth bass (D4) and one for feed-fed mandarin fish (D5).

[0039] Feeding conditions: The oxygen content in the water is greater than 5 mg / L, and the pH value is 7.80±0.5.

[0040] Grouping: The California bass in the California bass pond (D4) were grouped into: Effect Example 1 group (with the traditional Chinese medicine compound of Example 1 added), Effect Example 2 group (with the traditional Chinese medicine compound of Example 2 added), Effect Example 3 group (with the traditional Chinese medicine compound of Example 3 added), Effect Example 4 group (with the traditional Chinese medicine compound of Example 4 added), Effect Example 5 group (with the traditional Chinese medicine compound of Example 5 added), Effect Example 6 group (with the traditional Chinese medicine compound of Example 6 added), and Comparative Example 1 group; The mandarin fish in the feed mandarin fish pond (D5) were grouped into: Effect Example 7 group (with the traditional Chinese medicine compound of Example 1 added), Effect Example 8 group (with the traditional Chinese medicine compound of Example 2 added), Effect Example 9 group (with the traditional Chinese medicine compound of Example 3 added), Effect Example 10 group (with the traditional Chinese medicine compound of Example 4 added), Effect Example 11 group (with the traditional Chinese medicine compound of Example 5 added), Effect Example 12 group (with the traditional Chinese medicine compound of Example 6 added), and Comparative Example 2 groups.

[0041] Examples of efficacy: Groups 1-12: California bass weighing 300-500g and mandarin fish weighing 1-1.2kg, with varying degrees of abnormalities visible in the liver.

[0042] Blank control group (comparative example 1 and comparative example 2): California bass weighing 500-800g and mandarin fish weighing 1.5-2kg, with varying degrees of abnormalities visible in their livers.

[0043] Each group consists of three parallel groups. In Examples 1-6 and Comparative Example 1, 60 California bass were placed in each parallel group. In Examples 7-12 and Comparative Example 2, 60 mandarin fish were placed in each parallel group.

[0044] Feeding method: The base has two earthen ponds (4 mu of water surface): a D4 California bass pond and a D5 feed mandarin fish pond. Feed is mixed with feed and administered as a treatment. The dosage is 2g / kg of feed (Chinese herbal compound / feed), administered for 3 consecutive days followed by a 2-day break, constituting one course of treatment, for a total of 3 courses. The control group is fed a basic feed twice daily, at 6:00 AM and 6:00 PM.

[0045] 2. Clinical symptom observation

[0046] The California bass and mandarin fish in the aforementioned fish farm all exhibited abnormal livers. Visually, the livers showed varying degrees of discoloration (white liver, yellow liver, mottled liver, etc.), atrophy, and enlargement. Simultaneously, the livers became fragile and inelastic. (See the anatomical diagram of the liver shown.) Figure 3 .

[0047] In pond D4, after feeding the herbal compound from Examples 1-6 for 13 days, dissection revealed that the livers of the California bass were uniformly colored, bright red, and elastic. (See the anatomical diagram of the liver shown below.) Figure 1 In the D5 feed-fed mandarin fish pond, after feeding with the herbal compound formulas from Examples 1-6 for 15 days, dissection revealed that the livers of the feed-fed mandarin fish were uniformly colored, bright red, and elastic. (See the anatomical diagram of the liver shown below.) Figure 2 .

[0048] 3. Clinical efficacy assessment

[0049] (1) Judgment criteria

[0050] Cured: The aquatic animals are in good condition, have a strong appetite, and their livers are normal in color and elastic, with no abnormalities found.

[0051] Significant effect: Aquatic animals are in good condition, have strong feeding ability, and their livers are normal in color and elastic. Occasionally, 1-2 animals may show abnormal liver phenomena.

[0052] Effective: Aquatic animals are in good condition, have a strong appetite, and their livers are normal in color and elastic. A small number of animals may show abnormalities in their livers.

[0053] Ineffective: No change in symptoms.

[0054] Table 2 Experimental Results Record Sheet

[0055] Group Cure rate % apparent efficiency % Efficiency % Inefficiency % Overall effectiveness % Example 1 65 14 13 8 92 Example 2 67 18 5 10 90 Example 3 72 12 10 6 94 Example 4 60 20 9 11 89 Example 5 64 13 11 12 88 Example 6 65 18 12 5 95 Example 7 57 21 15 7 93 Example 8 60 10 14 16 84 Example 9 68 12 11 9 91 Example 10 59 19 9 13 87 Example 11 70 8 17 5 95 Example 12 60 16 9 15 85 Comparative Example 1 16 9 13 62 38 Comparative Example 2 12 14 17 57 43

[0056] As shown in Table 2, compared with Comparative Examples 1 and 2, the cure rate of Effective Examples 1-12 can reach over 57%, with a high of 72%; the total effective rate can reach over 84%, with a high of 95%. This indicates that the traditional Chinese medicine compound can be used to treat symptoms such as liver enlargement, atrophy, and abnormal color caused by spoiled feed and some bacterial infections, and has a good liver-protecting effect.

[0057] In summary, this invention uses rhubarb, dried tangerine peel, and codonopsis as the principal herbs, perilla, artemisia capillaris, and scutellaria barbata as the assistant herbs, forsythia and poria cocos as adjuvant herbs, and licorice as the guiding herb. The method of use is to mix the herb with feed and feed it to aquatic animals during their rapid growth period, administering it for 3-5 consecutive days, followed by a 2-3 day break, constituting one course of treatment. It is used to treat symptoms such as liver enlargement, atrophy, and abnormal color caused by spoiled feed and certain bacterial infections. This invention uses a plant-derived traditional Chinese medicine formula, making it safe to use, leaving no harmful residues, and eliminating environmental pollution and drug resistance issues. It is also suitable for use in modern high-density, intensive farming models. Furthermore, this herbal formula improves feed utilization and growth rate, specifically by enhancing the body's immune function, improving liver metabolism, and enhancing the integrity of intestinal digestion and absorption.

[0058] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for protecting the liver of aquaculture animals, characterized in that it comprises the following components: The traditional Chinese medicine composition is prepared from the following raw materials by weight: 16±2 parts of rhubarb, 18±2 parts of dried tangerine or orange peel, 18±2 parts of perilla, 30±2 parts of capillary yellow herb, 20±2 parts of forsythia, 18±2 parts of tuckahoe, 16±2 parts of Chinese ginseng, and 20±10 parts of licorice. ​ 2. The traditional Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials by weight: 16±1 parts of rhubarb, 18±1 parts of dried tangerine or orange peel, 18±1 parts of perilla, 30±1 parts of capillary yellow herb, 20±1 parts of forsythia, 18±1 parts of tuckahoe, 16±1 parts of Chinese ginseng, and 20±10 parts of licorice.

3. The preparation method of the traditional Chinese medicine composition for protecting and preserving the liver of aquaculture animals according to claim 1 or 2, characterized in that: The method comprises the following steps: The rhubarb, dried tangerine or orange peel, perilla, capillary yellow herb, forsythia, tuckahoe, Chinese ginseng, and licorice are dried, crushed, sieved, and mixed to obtain the traditional Chinese medicine composition for protecting liver.

4. The preparation method of claim 3, wherein: The drying temperature is 60-70°C. The sieving is performed through a 60-100 mesh sieve.

5. Use of the traditional Chinese medicine composition of claim 1 or 2 in the preparation of feed for protecting liver of aquaculture animals.

6. The use of claim 5, wherein: The aquaculture animals are in the fast growth period.

7. The use of claim 5, wherein: The aquaculture animals include fish.

8. The use of claim 5, wherein: The aquaculture animals include at least one of California bass and golden mandarin fish.

9. The use of any one of claims 5-8, wherein: The amount of the traditional Chinese medicine composition added to the feed is 2±1 g / kg.

Images