Application of taurine in promoting growth, inhibiting bacteria and inducing resistance of panax notoginseng

Abstract

The application provides application of taurine in promoting growth of Panax notoginseng, inhibiting bacteria, inducing Panax notoginseng to produce resistance and / or relieving Panax notoginseng continuous cropping obstacles, and further provides a method for promoting growth of Panax notoginseng, inhibiting bacteria, inducing Panax notoginseng to produce resistance and / or relieving Panax notoginseng continuous cropping obstacles, and a biological pesticide, a Panax notoginseng resistance inducer, a Panax notoginseng growth regulator and a Panax notoginseng continuous cropping obstacle reliever. Research shows that within a certain concentration range, taurine can significantly promote the growth of Panax notoginseng, inhibit the growth of the root rot pathogen of Panax notoginseng, reduce the occurrence of root rot, significantly relieve the continuous cropping obstacles of Panax notoginseng and enhance the resistance of Panax notoginseng to Alternaria alternata, and significantly reduce the disease area of Alternaria alternata. Moreover, taurine has the advantages of low toxicity, no pollution, environmental friendliness and the like, and is an effective way for green ecological prevention and control of Panax notoginseng.

Description

Technical Field

[0001] This invention belongs to the agricultural field, specifically relating to the application of taurine in promoting the growth of Panax notoginseng, inhibiting bacteria, inducing resistance in Panax notoginseng, and / or alleviating continuous cropping obstacles in Panax notoginseng; this invention also relates to a method for promoting the growth of Panax notoginseng, inhibiting bacteria, inducing resistance in Panax notoginseng, and / or alleviating continuous cropping obstacles in Panax notoginseng, as well as a biological pesticide, a Panax notoginseng resistance inducer, a Panax notoginseng growth regulator, and a Panax notoginseng continuous cropping obstacle alleviator. Background Technology

[0002] Panax notoginseng (Burk.) FHChen, belonging to the genus Panax in the Araliaceae family, is a unique and widely used medicinal crop in my country. It possesses properties such as dispersing blood stasis, stopping bleeding, reducing swelling, and relieving pain, and has significant therapeutic effects on cardiovascular and cerebrovascular diseases. Yunnan Province accounts for over 98% of the national Panax notoginseng cultivation area, and its cultivation and processing have become a crucial pillar industry for Yunnan's local economic development. Yunnan is a major producing area of ​​Panax notoginseng, but due to its growth habits, it is prone to severe leaf and root diseases, such as black spot and root rot. Furthermore, its continuous cropping obstacles are serious, all of which have become key factors restricting the healthy development of the Panax notoginseng industry, severely impacting its yield and quality. Currently, the main methods for alleviating these problems include chemical control, crop rotation, and biological control. The environmental harm of chemical control limits its application in actual production; while crop rotation cycles are long, and suitable crops for alleviating continuous cropping obstacles in Panax notoginseng are not yet used in production.

[0003] Taurine is a free β-sulfur-containing amino acid found in living organisms. Because it was first discovered in bovine bile, it is also known as taurine acid or taurine bile extract. Existing research indicates that taurine is an active substance that regulates normal physiological activities, possessing a wide range of biological functions, including anti-inflammatory and analgesic effects, maintaining osmotic pressure balance, regulating nerve conduction, participating in endocrine activity, regulating lipid digestion and absorption, increasing cardiac contractility, enhancing immunity, and strengthening cell membrane antioxidant capacity. Therefore, taurine has high medicinal and therapeutic value and is widely used in functional beverages, pet food, health foods, feed, and pharmaceuticals. Furthermore, it can also be used as a synthetic intermediate for biochemical reagents and other high-value-added products, making it a fine chemical with broad application value.

[0004] However, to date, there have been no reports of taurine promoting the growth of Panax notoginseng, inhibiting bacteria, inducing resistance in Panax notoginseng, or alleviating the obstacles of continuous cropping of Panax notoginseng. Summary of the Invention

[0005] The purpose of this invention is to provide an application of taurine in promoting the growth of Panax notoginseng, inhibiting bacteria, inducing resistance in Panax notoginseng and / or alleviating the obstacles of continuous cropping of Panax notoginseng, as well as related methods and agents.

[0006] This invention discovers that taurine can promote the growth of Panax notoginseng, induce resistance in Panax notoginseng, inhibit the growth of Panax notoginseng pathogens, and alleviate the obstacle of continuous cropping of Panax notoginseng. It is a potential biological pesticide, Panax notoginseng resistance inducer, Panax notoginseng growth regulator and Panax notoginseng continuous cropping obstacle alleviater. It has the advantages of low toxicity, no pollution and environmental friendliness, and is an effective way to control Panax notoginseng in a green and ecological way.

[0007] Specifically, the present invention is achieved through the following technical solutions:

[0008] In a first aspect, the present invention provides the application of taurine in promoting the growth of Panax notoginseng, inhibiting bacteria, inducing resistance in Panax notoginseng and / or alleviating the obstacles of continuous cropping of Panax notoginseng.

[0009] In a preferred embodiment, the antibacterial effect includes inhibiting pathogens of Panax notoginseng, such as the pathogens causing root rot of Panax notoginseng.

[0010] Preferably, the antibacterial activity includes inhibiting *Ilyonectria destructans*, *Fusarium solani*, *Monographella cucumerina*, and *Phytophthoracactorum*.

[0011] In a preferred embodiment, inducing resistance in Panax notoginseng includes inducing disease resistance in Panax notoginseng, which includes resistance to Panax notoginseng pathogens.

[0012] Preferably, the disease resistance includes resistance to Panax notoginseng black spot fungus.

[0013] In a preferred embodiment, the application includes treating Panax notoginseng with taurine, including drenching the roots or foliar spraying the Panax notoginseng plants with taurine.

[0014] In a preferred embodiment, taurine can be formulated with excipients into agriculturally acceptable formulations, including water-based solvents, sprays, etc.

[0015] Preferably, taurine is used in the form of an aqueous solution.

[0016] The concentration of the taurine solution can be from 0.01 to 100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL, 60 μg / mL, 70 μg / mL, 80 μg / mL, 90 μg / mL, or 100 μg / mL. More preferably, the concentration of the taurine solution can be from 0.1 to 100 μg / mL. When used to alleviate the obstacles of continuous cropping of Panax notoginseng, the concentration of taurine solution can be 0.01 μg / mL to 100 μg / mL; when used for antibacterial purposes, the concentration of taurine solution can be 0.1 μg / mL to 100 μg / mL; when used to promote the growth of Panax notoginseng, the concentration of taurine solution can be 0.01 to 100 μg / mL; when used to induce resistance in Panax notoginseng, the concentration of taurine solution can be 0.01 to 100 μg / mL.

[0017] In a second aspect, the present invention provides a method for promoting the growth of Panax notoginseng, inhibiting bacteria, inducing resistance in Panax notoginseng and / or alleviating the obstacle of continuous cropping of Panax notoginseng, the method comprising treating Panax notoginseng with taurine.

[0018] In a preferred embodiment, the antibacterial effect includes inhibiting pathogens of Panax notoginseng, such as the pathogens causing root rot of Panax notoginseng.

[0019] Preferably, the antibacterial activity includes inhibiting *Ilyonectria destructans*, *Fusarium solani*, *Monographella cucumerina*, and *Phytophthoracactorum*.

[0020] In a preferred embodiment, inducing resistance in Panax notoginseng includes inducing disease resistance in Panax notoginseng, which includes resistance to Panax notoginseng pathogens.

[0021] Preferably, the disease resistance includes resistance to Panax notoginseng black spot fungus.

[0022] In a preferred embodiment, the application includes treating Panax notoginseng with taurine, including drenching the roots or foliar spraying the Panax notoginseng plants with taurine.

[0023] In a preferred embodiment, taurine can be formulated with excipients into agriculturally acceptable formulations, including water-based solvents, sprays, etc.

[0024] Preferably, taurine is used in the form of an aqueous solution.

[0025] The concentration of the taurine solution can be from 0.01 to 100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL, 60 μg / mL, 70 μg / mL, 80 μg / mL, 90 μg / mL, or 100 μg / mL. More preferably, the concentration of the taurine solution can be from 0.1 to 100 μg / mL. When used to alleviate the obstacles of continuous cropping of Panax notoginseng, the concentration of taurine solution can be 0.01 μg / mL to 100 μg / mL; when used for antibacterial purposes, the concentration of taurine solution can be 0.1 μg / mL to 100 μg / mL; when used to promote the growth of Panax notoginseng, the concentration of taurine solution can be 0.01 to 100 μg / mL; when used to induce resistance in Panax notoginseng, the concentration of taurine solution can be 0.01 to 100 μg / mL.

[0026] In a third aspect, the present invention provides a biological pesticide, a Panax notoginseng resistant inducer, a Panax notoginseng growth regulator, and a Panax notoginseng continuous cropping obstacle alleviater, wherein the biological pesticide, Panax notoginseng resistant inducer, Panax notoginseng growth regulator, and Panax notoginseng continuous cropping obstacle alleviater contain taurine as an active ingredient.

[0027] In this invention, the biopesticide refers to a preparation that uses living organisms, biologically active substances produced by biological metabolism, or substances extracted from organisms to prevent and control diseases, pests, weeds, and rodents in agricultural and forestry crops, and can be commercially marketed and circulated. It includes antibacterial agents, biological insecticides, agricultural antibiotics, and ecological pesticides.

[0028] In a preferred embodiment, the biopesticide includes a bacteriostatic agent for inhibiting pathogens of Panax notoginseng, such as the pathogen causing root rot of Panax notoginseng; preferably, the bacteriostatic agent includes inhibition of Ilyonectriadestructans, Fusarium solani, Monographella cucumerina, and Phytophthora cactorum.

[0029] In this invention, the Panax notoginseng growth regulator is a type of substance that regulates various metabolic processes within Panax notoginseng to improve its quality and increase its yield.

[0030] In a preferred embodiment, the Panax notoginseng growth regulator is used to promote the growth of Panax notoginseng.

[0031] In this invention, promoting the growth of Panax notoginseng includes promoting seed germination, biomass accumulation, root growth, improving plant quality, and increasing yield.

[0032] In this invention, the term "Sanqi elicitor" refers to a general term for factors that can activate Sanqi to produce a defensive response.

[0033] In a preferred embodiment, the Panax notoginseng resistance inducer is used to induce resistance in Panax notoginseng, including inducing disease resistance, said disease resistance including resistance to Panax notoginseng pathogens. Preferably, the disease resistance includes resistance to Panax notoginseng black spot fungus.

[0034] In this invention, the Panax notoginseng continuous cropping obstacle alleviator refers to a class of substances that can alleviate the abnormal growth and development of Panax notoginseng caused by continuous cultivation of Panax notoginseng on the same piece of soil.

[0035] In a preferred embodiment, the bio-antibacterial agent, Panax notoginseng antagonist, Panax notoginseng growth regulator, and Panax notoginseng continuous cropping obstacle reliever may also include other excipients and may be water-soluble, spray, etc.

[0036] Preferably, taurine is used in the form of an aqueous solution.

[0037] The concentration of the taurine solution can be from 0.01 to 100 μg / mL, preferably 0.01 μg / mL, 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL, 60 μg / mL, 70 μg / mL, 80 μg / mL, 90 μg / mL, or 100 μg / mL. More preferably, the concentration of the taurine solution can be from 0.1 to 100 μg / mL. When used to alleviate the obstacles of continuous cropping of Panax notoginseng, the concentration of taurine solution can be 0.01 μg / mL to 100 μg / mL; when used for antibacterial purposes, the concentration of taurine solution can be 0.1 μg / mL to 100 μg / mL; when used to promote the growth of Panax notoginseng, the concentration of taurine solution can be 0.01 to 100 μg / mL; when used to induce resistance in Panax notoginseng, the concentration of taurine solution can be 0.01 to 100 μg / mL.

[0038] Compared with the prior art, the present invention has the following advantages:

[0039] This invention discloses the application of taurine in promoting the growth of Panax notoginseng, inhibiting bacteria, inducing resistance in Panax notoginseng, and / or alleviating continuous cropping obstacles in Panax notoginseng. Studies have found that within a certain concentration range, taurine can significantly promote the growth of Panax notoginseng, inhibit the growth of root rot pathogens and reduce the occurrence of root rot, significantly alleviate continuous cropping obstacles in Panax notoginseng, enhance its resistance to black spot disease, and significantly reduce the affected area of ​​black spot disease. Furthermore, it has advantages such as low toxicity, no pollution, and environmental friendliness, making it an effective approach for the green and ecological control of Panax notoginseng. Attached Figure Description

[0040] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings (*p<0.05):

[0041] Figure 1 The effect of taurine on Panax notoginseng cuttings transplanted in continuously cropped soil.

[0042] Figure 2 The effect of taurine on Panax notoginseng seeds grown in continuously cropped soil.

[0043] Figure 3 The study investigated the antibacterial effect of taurine on the main pathogens causing root rot in Panax notoginseng.

[0044] Figure 4 The effect of taurine on the growth of Panax notoginseng.

[0045] Figure 5 The image shows the area of ​​lesions induced by taurine drenching in Panax notoginseng to resist black spot disease.

[0046] Figure 6 A graph showing the area of ​​lesions induced by taurine foliar spraying to induce resistance to black spot disease in Panax notoginseng. Detailed Implementation

[0047] The embodiments of the present invention are described in detail below. The embodiments are provided to better illustrate the content of the present invention and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0048] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0049] Example 1: Evaluation test of the effect of taurine in alleviating the continuous cropping obstacles of Panax notoginseng

[0050] Take 0.1g of taurine standard (purity >99%) and add it to a blue-mouth bottle containing 1L of sterile water. Mix well and prepare a taurine solution with a concentration of 100μg / mL. Then take 100mL of the taurine solution with a concentration of 100μg / mL and add it to a blue-mouth bottle containing 900mL of sterile water to prepare a taurine solution with a final concentration of 10μg / mL. Repeat this process to prepare taurine solutions with concentrations of 1μg / mL, 0.1μg / mL, and 0.01μg / mL for later use. (1) Mix the soil from the continuous cropping of Panax notoginseng that has been planted for one year and then pot it. Select Panax notoginseng seedlings with uniform growth and transplant them. After the seedlings have recovered for one week, prepare a 50mL graduated cylinder and take out the corresponding concentration of taurine solution to irrigate the roots of the Panax notoginseng seedlings in the continuous cropping soil. Use sterile water as a control. Treat 6 pots for each concentration, with 50mL per pot. Fifteen days later, irrigate again with taurine solution. Two months later, the survival rate of Panax notoginseng seedlings and the incidence of root rot were statistically analyzed. The results are as follows: Figure 1 As shown. (2) After mixing the soil from the continuous cropping of Panax notoginseng for one year, fill pots, select plump Panax notoginseng seeds for sowing, and prepare a 50mL graduated cylinder. Take out the corresponding concentration of taurine solution for root irrigation treatment, with sterile water as the control. Treat 8 pots for each concentration, with 50mL per pot. Irrigate with taurine solution again after 15 days. After emergence, count the emergence rate. After 2 months, count the survival rate and the incidence of root rot. The results are as follows. Figure 2 As shown.

[0051] Seedling survival rate = (Number of seedlings remaining / Total number) * 100%

[0052] Root rot incidence rate = (Number of cases / Total number) * 100%

[0053] Germination rate = (Number of germinated seedlings / Total number) * 100%

[0054] from Figure 1 It can be seen that treatment with taurine solutions at concentrations ranging from 0.01 μg / mL to 100 μg / mL on transplanted Panax notoginseng cuttings from continuously cropped soil can increase the survival rate of Panax notoginseng seedlings. Figure 1 A), among which 1 μg / mL taurine solution showed the best treatment effect; it can also reduce the incidence of root rot in Panax notoginseng. Figure 1 B) Among them, the treatment with 1 μg / mL taurine solution showed the best effect. This indicates that taurine has a significant alleviating effect on the continuous cropping obstacle of Panax notoginseng.

[0055] from Figure 2 It can be seen that treating Panax notoginseng seeds with taurine solutions at concentrations ranging from 0.1 μg / mL to 100 μg / mL can increase the germination rate of Panax notoginseng seeds in continuously cropped soil. Figure 2 A), among which the treatment with 0.1 μg / mL taurine solution was the most effective; it could also improve the survival rate of Panax notoginseng seedlings. Figure 2B), among which 1 μg / mL taurine solution showed the best treatment effect; it can also reduce the incidence of root rot in Panax notoginseng. Figure 2 C) Among them, the treatment with 1 μg / mL taurine solution showed the best effect. This indicates that taurine has a significant alleviating effect on the continuous cropping obstacle of Panax notoginseng.

[0056] Example 2: Antibacterial test of taurine against the main root rot pathogens of Panax notoginseng

[0057] Prepare 6 sterilized centrifuge tubes (size: 15mL). Add 0.1g of taurine to a centrifuge tube containing 10mL of sterile water to prepare a taurine solution with a concentration of 10mg / mL. After mixing, take 1mL of the solution and add it to a centrifuge tube containing 9mL of sterile water to prepare a taurine solution with a concentration of 1mg / mL. Repeat this process to prepare taurine solutions with concentrations of 0.1mg / mL, 0.01mg / mL, and 0.001mg / mL for later use. Prepare 6 250mL Erlenmeyer flasks and place 100mL of PDA (potato agarose medium) in each flask. Take 1 mL of the prepared taurine solution and add it to sterilized PDA (potato agarose medium) to make plates with final concentrations of 0.01 μg / mL, 0.1 μg / mL, 1 μg / mL, 10 μg / mL, and 100 μg / mL. Use the same volume of sterile water in PDA medium as a control. The main root rot pathogens of Panax notoginseng, *Ilyonectria destructans* (strain number: RS6), *Fusarium solani* (strain number: F3), *Monographella cucumerina* (strain number: M), and *Phytophthoracactorum* (strain number: D-6), were cultured on PDA (potato agarose medium). After 7 days of culture, holes were punched around the outermost ring of the pathogens using a 6 mm punch. The pathogens were then inoculated into the center of the PDA medium and incubated in the dark at 25°C. Each treatment was replicated six times. When the control colonies reached 2 / 3 of the plate's height, the diameter of each colony was measured using the "cross-cross" method. The results are shown below. Figure 3 As shown.

[0058] from Figure 3 It can be seen that taurine solutions with concentrations of 0.1 μg / mL to 100 μg / mL can inhibit the growth of pathogens causing root rot in Panax notoginseng and reduce the occurrence of root rot.

[0059] Example 3: Experiment on the effect of taurine on the growth of Panax notoginseng

[0060] Take 0.1g of taurine standard (purity >99%) into a blue-mouth bottle containing 1L of sterile water, mix well, and prepare a taurine solution with a concentration of 100μg / mL. Then take 100mL of the 100μg / mL taurine solution and add it to a blue-mouth bottle containing 900mL of sterile water to prepare a taurine solution with a final concentration of 10μg / mL. Repeat this process to prepare taurine solutions of 1μg / mL, 0.1μg / mL, and 0.01μg / mL for later use. (1) Prepare a 50mL graduated cylinder and take out the corresponding concentration of taurine solution to irrigate the roots of Panax notoginseng seedlings planted in sterile soil with uniform growth. Use sterile water as a control. Treat 8 pots with each concentration, 50mL per pot. Irrigate with taurine solution again every 21 days for a total of three treatments. After 1 month, count the height of individual plants. The results are as follows. Figure 4 As shown.

[0061] from Figure 4 It can be seen that, compared with the blank control, under sterilization conditions, taurine solutions of 0.01–100 μg / mL can significantly promote the plant height of Panax notoginseng, indicating that taurine can significantly promote the growth of Panax notoginseng.

[0062] Example 4: Experiment on taurine-induced resistance of Panax notoginseng to black spot fungus

[0063] Take 0.1g of taurine standard (purity >99%) and add it to a blue-mouth bottle containing 1L of sterile water. Mix well to prepare a taurine solution with a concentration of 100μg / mL. Then, take 100mL of the 100μg / mL taurine solution and add it to a blue-mouth bottle containing 900mL of sterile water to prepare a taurine solution with a final concentration of 10μg / mL. Repeat this process to prepare taurine solutions with concentrations of 1μg / mL, 0.1μg / mL, and 0.01μg / mL for later use. (1) Select healthy Panax notoginseng potted plants after transplanting, remove weeds from the soil surface, water thoroughly and leave for 1 day. Then, use a 50mL graduated cylinder to measure 50mL of taurine solution of the corresponding concentration and apply it to the roots of the Panax notoginseng potted plants for root irrigation. Use sterile water as a control. After 1 day of treatment, inoculate the leaves with a fungal cake of A. panax (strain number: SL17) for black spot disease and transfer them to a transparent plastic incubator for moist cultivation. After 4 days of inoculation and disease onset, collect diseased leaves and scan the diseased leaves using an Epson Perfection V850 Pro scanner. Then, use Adobe Photoshop software to crop and calculate the relative lesion area between the induction treatment and the control treatment to evaluate the effect of taurine in inducing resistance in Panax notoginseng. Figure 5As shown. (2) Select Panax notoginseng potted plants that are growing well after transplanting, remove weeds from the soil surface, take out the Panax notoginseng seedlings, wash the roots with running water, avoid damaging the roots when washing, and place them in water for 1 day to allow them to recover. Then, use a 10mL spray bottle to take 10mL of taurine solution of the corresponding concentration and spray it onto the leaves of Panax notoginseng. Use sterile water as a control. After 1 day of treatment, challenge the leaves with the fungal cake of black spot fungus A. panax (strain number: SL17) and transfer them to a transparent plastic incubator for moist culture. After 4 days of inoculation and disease onset, collect the diseased leaves, scan the diseased leaves with an Epson Perfection V850 Pro scanner, and then use Adobe Photoshop software to cut and calculate the relative lesion area of ​​the induction treatment and the control treatment to evaluate the effect of taurine in inducing resistance in Panax notoginseng. Figure 6 As shown.

[0064] from Figure 5 It can be seen that taurine solutions with concentrations of 0.01–100 μg / mL applied to the roots of Panax notoginseng can significantly reduce the affected area of ​​black spot disease.

[0065] from Figure 6 It can be seen that taurine solutions with concentrations of 0.01–100 μg / mL can also significantly reduce the affected area of ​​Panax notoginseng black spot disease after being sprayed on the leaves.

[0066] In conclusion, in the cultivation of Panax notoginseng, taurine solutions with a concentration of 0.1–100 μg / mL can significantly promote the growth of Panax notoginseng, inhibit the growth of root rot pathogens and reduce the occurrence of root rot, significantly alleviate the continuous cropping obstacles of Panax notoginseng, enhance its resistance to black spot disease, and significantly reduce the affected area of ​​black spot disease.

[0067] Obviously, the above embodiments are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art can make various modifications and variations to the present invention without departing from the spirit and scope of the invention. Therefore, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention also intends to include these modifications and variations.

Claims

1. An application of taurine in alleviating the obstacle of continuous cropping of Panax notoginseng, wherein the taurine is prepared in the form of an aqueous solution with a concentration of 0.01 to 100 µg / mL.

2. The application according to claim 1, characterized in that, The concentration of taurine solution is 0.1–100 µg / mL.

3. The application according to claim 1 or 2, characterized in that, The applications include applying taurine to the roots or foliar spray of Panax notoginseng.

4. A method for alleviating the obstacle of continuous cropping of Panax notoginseng, characterized in that, The method includes treating Panax notoginseng with taurine, wherein the taurine is prepared as an aqueous solution and the concentration of the taurine solution is 0.01–100 µg / mL.

5. The method according to claim 4, characterized in that, The concentration of taurine solution is 0.1–100 µg / mL.

6. The method according to claim 5, characterized in that, The method includes applying taurine to the roots or foliar spray of Panax notoginseng.

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