A fungicidal composition for controlling xanthomonas campestris pv. oryzae

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a kind of fungicide composition for preventing and treating bacterial leaf streak of rice. The fungicide composition for preventing and treating bacterial leaf streak of rice is composed of amino acid and auristatin or paclobutrazol; the mass ratio of the amino acid and auristatin is 1-7:14-1; the mass ratio of the amino acid and paclobutrazol is 1-20:8-1. When L-pyroglutamic acid is used in combination with auristatin or paclobutrazol, the effect of synergism is found, and compared with single active ingredient, the prevention and treatment effect on bacterial leaf streak of rice can be improved, and the new high-efficiency fungicide can be developed. In addition, compared with single active ingredient, the fungicide composition for preventing and treating bacterial leaf streak of rice can reduce the risk of drug resistance, and is safe.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticides, and particularly relates to a fungicidal composition for preventing and treating bacterial leaf streak of rice. BACKGROUND

[0002] Ginucin is an adenosine-containing nucleoside antibiotic obtained by culturing and fermenting Streptomyces aureus Suzhou variant, belongs to a microbial source fungicide, has excellent effects on various bacterial diseases, is safe to human, livestock and environment, is an agricultural antibiotic for preventing and treating bacterial plant diseases, and has good prevention and treatment effects on bacterial diseases such as citrus canker caused by Xanthomonas campestris, bacterial leaf streak of rice and bacterial leaf blight of rice.

[0003] PP333 is a plant growth regulator, has effects of delaying plant growth, inhibiting stem elongation, shortening internodes, promoting plant tillering, increasing plant stress resistance and improving crop yield, and is suitable for plants such as rice, wheat, peanuts, fruit trees, tobacco, soybeans and flowers. According to research reports, PP333 has effects on plant diseases. The patent with publication number CN104488893B discloses that the co-toxicity coefficient of a mixture of flutriafol and PP333 for preventing and treating soft rot of Chinese cabbage is greater than 120, and the mixture shows obvious synergistic effect.

[0004] L-pyroglutamic acid, CAS: 98-79-3, molecular formula: C5H7NO3, and structural formula as follows:

[0005]

[0006] According to research reports, L-pyroglutamic acid can be used for preventing and treating crop diseases. The patent with publication number CN107467029A discloses an amino acid agricultural composition, the fungicidal active ingredient of which is L-pyroglutamic acid, which has high fungicidal activity on crop diseases including cucumber downy mildew, potato late blight and grape downy mildew. Moreover, L-pyroglutamic acid belongs to an amino acid compound, can promote plant growth, enhance the immunity of crops to diseases, and is safe to crops.

[0007] When using pesticides to prevent and treat plant diseases, long-term use of a single active ingredient pesticide can easily cause plant pathogens to develop drug resistance, resulting in reduced or even lost efficacy. Screening of a compound formula with synergistic effect can delay the development of drug resistance of plant pathogens and improve the prevention and treatment effect. Therefore, it is of great significance to screen a compound formula of L-pyroglutamic acid.

[0008] The information disclosed in this BACKGROUND section is only intended to increase an understanding of the general context of the application and should not be taken as an acknowledgement that this information constitutes prior art that is already known in the art. SUMMARY

[0009] The present application aims to provide a bactericidal composition for preventing and treating Xanthomonas oryzae pv. oryzae.

[0010] To achieve the above object, the present application provides the following technical solutions.

[0011] A bactericidal composition for preventing and treating Xanthomonas oryzae pv. oryzae, wherein the effective component of the bactericidal composition is composed of amino acid and kasugamycin or paclobutrazol; the mass ratio of the amino acid to the kasugamycin is 1-7:14-1; and the mass ratio of the amino acid to the paclobutrazol is 1-20:8-1.

[0012] More specifically, the amino acid is L-pyroglutamic acid.

[0013] More specifically, the mass ratio of the amino acid to the kasugamycin is 1:3.

[0014] More specifically, the mass ratio of the amino acid to the paclobutrazol is 1:8 or 20:1.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] (1) The present application finds that L-pyroglutamic acid has a synergistic effect when used in combination with kasugamycin or paclobutrazol, and can improve the prevention and treatment effect on Xanthomonas oryzae pv. oryzae compared with a single effective component, and can be used for developing a new type of high-efficiency bactericide.

[0017] (2) The present application can reduce the risk of drug resistance compared with a single effective component, and has safety. DETAILED DESCRIPTION

[0018] The following clearly and completely describes the technical solutions of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0019] Examples : Virulence determination test

[0020] 1. Culture medium

[0021] NA culture medium: polyprotein peptone 6 g, yeast powder 1 g, beef extract 3 g, sucrose 15 g, agar 17 g, distilled water to 1 L, and adjusted to pH 7.0 with 10 mol / L NaOH.

[0022] NB medium: poly-peptone 6g, yeast powder 1g, beef extract 3g, sucrose 15g, distilled water to 1L, and adjust to pH 7.0 with 10 mol / L NaOH.

[0023] 2. Target pathogen

[0024] Xanthomonas oryzae pv. oryzicola, isolated from diseased rice plants.

[0025] The target pathogen was activated and cultured in a 30℃ bacterial incubator for 48h, and then a single colony was picked and cultured in 30mL NB medium at 30℃, 120r / min, and the OD 600 value was adjusted to about 0.5 to obtain a target pathogen liquid.

[0026] 3. Test agents:

[0027] 98% L-pyroglutamic acid technical material (Hubei Zhenbo Chemical Co., Ltd.), 94% gancimycin (Shanghai Nongle Biological Products Co., Ltd.), 96% paclobutrazol technical material (Liaoning Shenglian Biological Technology Co., Ltd.)

[0028] After the test agents were dissolved, they were diluted with 0.1% Tween-80 aqueous solution to a single agent stock solution with a concentration of 1000mg / L, and multiple groups of ratios were set up, and each group of combined agents was set up with 5 series of mass concentration gradients by equal ratio method using 0.1% Tween-80 aqueous solution. The above agents were prepared and used immediately.

[0029] 4. Test method (reference: NT / T 1156.16-2008 Pesticide Laboratory Bioassay Test Guidelines Fungicides Part 16: Inhibition of Bacterial Growth Quantity Test Turbidity Method)

[0030] 1mL of the liquid was added to a container containing 20mL of NB medium to prepare NB medium containing different concentrations of the liquid, and then 200μL of the bacterial liquid was inoculated into the NB medium, 5 replicates were set up for each mass concentration of the liquid, and 0.1% Tween-80 aqueous solution was added as a blank control. Place in a 30℃, 120r / min incubator and shake culture until the OD 600 value of the blank control is about 0.5, then use a spectrophotometer to measure the OD 600 value of each agent-treated NB medium, and calculate the growth inhibition rate of the test pathogen.

[0031] In addition, the OD 600 value of the sterile NB medium with different concentrations of the liquid was measured during the test, as well as the OD 600 value of the sterile NB medium of the blank control, to correct the OD value caused by the agent itself.

[0032] Corrected OD value = OD value of NB medium containing bacteria - OD value of NB medium without bacteria

[0033]

[0034] 5. Data Analysis

[0035] The regression equations for the target pathogens and the EC50 values ​​for each combination of drugs were obtained. 50 The synergistic effect coefficient (SR) of the mixture was calculated using the Wadley method, and the synergistic effect of the drug mixture was evaluated based on the SR. The results are shown in Table 1-2.

[0036] Table 1. Efficacy evaluation of L-pyroglutamic acid combined with chlortetracycline against bacterial leaf streak of rice.

[0037]

[0038] Table 1 shows that the synergistic effect of L-pyroglutamic acid and chlortetracycline in controlling bacterial leaf streak of rice is greater than 1.5, indicating a synergistic effect. The product manual states that the combined use of L-pyroglutamic acid and chlortetracycline in this invention can improve the control effect against bacterial leaf streak of rice.

[0039] Table 2. Efficacy evaluation of L-pyroglutamic acid combined with paclobutrazol against bacterial leaf streak of rice.

[0040]

[0041]

[0042] Table 2 shows that the synergistic effect of L-pyroglutamic acid and paclobutrazol in controlling bacterial leaf streak of rice is greater than 1.5, indicating a synergistic effect. The product manual states that the combined use of L-pyroglutamic acid and paclobutrazol in this invention can improve the control effect against bacterial leaf streak of rice.

Claims

1. A fungicidal composition for controlling bacterial leaf streak of rice, characterized in that, The active ingredient of the bactericidal composition is composed of amino acids and chlortetracycline; wherein the mass ratio of the amino acids to chlortetracycline is 1-7:14-1, and the amino acid is L-pyroglutamic acid.

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