A method for cultivating zebra-patterned pearls

By targeted cultivation of the cuming oyster donor oyster and controlling the secretion ratio of nacre in the pearl sac, the problem of heterologous immune rejection between the donor oyster and the pearl cultivation oyster was solved, the cultivation of high-quality zebra-patterned pearls was achieved, and the economic value and color diversity of pearls were improved.

CN117502328BActive Publication Date: 2025-09-23SHANGHAI OCEAN UNIV
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Patent Information

Application Number
CN202311627674.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-11-30
Publication Date
2025-09-23
Estimated Expiration
2043-11-30

AI Technical Summary

Technical Problem

The existing technology has failed to effectively solve the problem of xenoimmune rejection between the donor oyster cell fragments and the pearl oysters, resulting in the difficulty in producing zebra pearls with a high pearl yield rate, and the quality and style of the pearls cannot meet consumer demand.

Method used

By directionally cultivating cuming oysters with different inner shell colors, small cell pieces of nacre of specific colors are obtained, and inserted into pearl oysters in the grooves of the bead nucleus according to a predetermined arrangement. Combined with the design of the bead nucleus made of polypropylene plastic, the secretion ratio and arrangement of nacre in the pearl sac are controlled, and combined with astaxanthin and β-carotene nutrition, high-quality zebra-striped pearls can be quickly cultivated.

Benefits of technology

It solves the problem of heterologous immune rejection between the supply oysters and the pearl cultivation oysters, ensures the cultivation of high-quality zebra-patterned pearls, improves the economic value and color brightness of pearls, and meets the market demand for pearl diversity.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention discloses a method for cultivating zebra pearls, comprising the following steps: first, directing the cultivation of Hyriopsis cumingii with different inner shell colors to prepare cell flakes that secrete nacre of a specific color; then, determining the bead nucleus size parameters according to the pearl oyster specifications, shaping a pearl sac prefabricated mold for producing zebra pearls, the mold being provided with grooves, the size and number of which are set according to the mold, and the cell flakes of different colors can be arranged according to a predetermined pattern; then, inserting the pearl sac prefabricated mold into the mantle of the pearl oyster, and prefabricating a pearl sac that can cultivate zebra pearls in 4-5 months; removing the pearl sac prefabricated mold, implanting the bead nucleus, and cultivating to obtain zebra pearls. The zebra pearls cultivated by the method of the present invention enrich the pearl tones and significantly improve the market competitiveness of pearls. The present invention not only reduces the difficulty and time cost of operation, but also can scientifically cultivate high-quality zebra pearls, thereby improving the economic value of the product.
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Description

Technical Field

[0001] The invention relates to the technical field of pearl cultivation, in particular to a method for cultivating zebra-patterned pearls. Background Art

[0002] Pearls, the product of biomineralization of mollusks, hold great collectible and aesthetic value. The uniqueness and diversity of pearl colors have always been a trendy pursuit among consumers. While pearls come in a wide variety of colors, they all primarily consist of a single color, each encompassing numerous shades of color. This has led to a limited monochromatic color palette in the jewelry market. While advanced dyeing techniques are available, these unnatural colors often fade, making them unpopular with consumers and failing to meet their demands.

[0003] Pearls on the market are primarily cultivated, primarily through the use of a nucleus insertion procedure to insert small cell fragments or bead nuclei, stimulating the formation of pearl sacs, which then secrete nacre. Currently, due to the issue of immune rejection between the donor oyster cell fragments and between the oysters, there is no method for producing zebra-striped pearls with a high yield. Consequently, the styles and quality of pearls cannot meet consumer demand, limiting the development of the pearl industry. Summary of the Invention

[0004] The present invention provides a method for cultivating zebra-patterned pearls, the purpose of which is to solve the problems raised in the background technology.

[0005] In order to solve the above problems, the present invention provides the following technical solutions:

[0006] A method for cultivating zebra-patterned pearls comprises the following steps:

[0007] (a) Directed cultivation of Hyriopsis cumingii donor oysters with different inner shell colors to obtain cell fragments that cause the pearl oysters to secrete nacre of a specific color;

[0008] (b) shaping the grooves in the bead core to accommodate different numbers of cell sheets;

[0009] (c) placing two cell fragments that control the secretion of nacre of a specific color by the pearl oyster in a predetermined arrangement in the groove of the bead nucleus, and inserting the bead nucleus into the mantle of the pearl oyster;

[0010] (d) quickly prefabricating a pearl capsule capable of cultivating zebra-patterned pearls, thereby achieving the cultivation of zebra-patterned pearls;

[0011] (e) After the cultivation is completed, the pearls are taken out and the quality of the zebra-patterned pearls can be judged based on the pearl size, the color of the front and back, the clarity of the zebra pattern, and the glossiness;

[0012] (f) The pearl capsule prefabricated mold is taken out and the pearl nucleus is implanted to produce zebra-patterned pearls for the second time.

[0013] Preferably, the pearl-producing clams and pearl-producing clams in step (a) are both Hyriopsis cumingii.

[0014] Preferably, the directed cultivation of Hyriopsis cumingii with different inner shell colors in step (a) is to obtain stable genetic supply clams through family selection and whole genome selection breeding, and the supply clams are directed cultivated by controlling the breeding environment and the conditions for raising algae.

[0015] Preferably, the grooved bead cores formed in step (b) to accommodate different numbers of cell flakes are made of polypropylene plastic.

[0016] Preferably, the size of the bead core in step (b) is adjusted according to the length of the pearl oyster. Since the bead core has an irregular shape, the maximum length of the bead core is defined as the length (X) of the bead core, and the maximum length perpendicular to the length is defined as the width (Y). Therefore, the functional relationship between the length and width of the bead core and the length (W) of the pearl oyster shell is:

[0017]

[0018] Preferably, the size of the bead core groove in step (c) is determined according to the size of the bead core and the number of grooves, and the groove length (γ) is: The groove width is calculated as a function of:

[0019]

[0020] Calculation is performed and the number of grooves (z) is:

[0021]

[0022] Wherein, the groove width is ω, the bead core length is X, and the bead core width is Y, and the unit is mm.

[0023] Preferably, in order to overcome the sliding problem of the cell flakes, the cell flakes are just filled into the groove of the bead nucleus to fix their position in the pearl oyster and quickly form high-quality zebra-patterned pearl sacs.

[0024] Preferably, in the cultivation of zebra-patterned pearls in step (d), the secretion ratio and arrangement of two specific colors of nacre in the pearl sac are controlled according to the size and position of the cell flakes, so as to achieve the color effect required by the market.

[0025] Preferably, the square-shaped bead nucleus is implanted in step (f). The tip of the crochet hook is used to hook the epidermis of the inner membrane from right to left at the inner membrane of the pearl oyster and make a small hole about 12 mm long. The one side serving as the fulcrum is placed at the top and the other side is placed at the bottom and slowly placed into the small hole.

[0026] Preferably, during the breeding and cultivation process, the top of the pearl oyster shell and the sail are placed under the net bag, with the surgical wound site facing upwards. In daily pearl breeding management, trace mineral elements, astaxanthin and β-carotene are regularly supplemented to ensure the nutrients necessary for the color formation of the pearl sac of the pearl oyster, making the color brighter and clearer. The color characteristics and glossiness of the front and back sides of the square pearls are taken out to judge the quality of the zebra-patterned pearl sac.

[0027] Beneficial effects:

[0028] 1. The donor clams and pearl oysters used in the present invention are both Hyriopsis cumingii, and the donor clams with different inner shell colors are obtained through the latest breeding methods such as whole genome selection breeding, and can be stably inherited, thereby solving the problem of heterologous immune rejection between the donor clams and the pearl oysters during the preparatory process of zebra-striped pearl sacs;

[0029] 2. The bead nucleus designed by the present invention to accommodate cell flakes can effectively avoid the problem of low-quality zebra pearl sacs and the resulting low-quality zebra pearls caused by the sliding of cell flakes, thereby ensuring the smooth cultivation of high-quality zebra pearls.

[0030] 3. The present invention controls the secretion ratio and arrangement of two specific colors of nacre in the pearl sac by regulating the size and position of cell flakes, thereby quickly prefabricating pearl sacs capable of cultivating high-quality two-color pearls, thereby improving the quality of the pearls and increasing the economic value of the product.

[0031] 4. The present invention designs a function to limit the size of the pearl nucleus and the number and size of the grooves according to the length of the pearl oyster shell, which not only reduces the difficulty and time cost of operation, but also can scientifically cultivate high-quality zebra-patterned pearls and improve the economic value of the product. DETAILED DESCRIPTION

[0032] The following is a detailed description of an embodiment of the present invention in conjunction with the accompanying drawings: This embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation method and specific operation process are given, but the protection scope of the present invention is not limited to the following embodiment.

[0033] A method for cultivating zebra-patterned pearls comprises the following steps:

[0034] (a) Directed cultivation of Hyriopsis cumingii donor oysters with different inner shell colors to obtain cell fragments that cause the pearl oysters to secrete nacre of a specific color;

[0035] (b) shaping the grooves in the bead core to accommodate different numbers of cell sheets;

[0036] (c) placing two cell fragments that control the secretion of nacre of a specific color by the pearl oyster in a predetermined arrangement in the groove of the bead nucleus, and inserting the bead nucleus into the mantle of the pearl oyster;

[0037] (d) quickly prefabricating a pearl capsule capable of cultivating zebra-patterned pearls, thereby achieving the cultivation of zebra-patterned pearls;

[0038] (e) After the cultivation is completed, the pearls are taken out and the quality of the zebra-patterned pearls can be judged based on the pearl size, the color of the front and back, the clarity of the zebra pattern, and the glossiness;

[0039] (f) The pearl capsule prefabricated mold is taken out and the pearl nucleus is implanted to produce zebra-patterned pearls for the second time.

[0040] The clams for supplying pearls and the clams for cultivating pearls in the step (a) are both Hyriopsis cumingii.

[0041] In the step (a), the directional cultivation of cuming clams with different inner shell colors is carried out by obtaining stable genetic clams through family selection and whole genome selection breeding, and the clams are directional cultivated by controlling the breeding environment and the conditions for feeding algae.

[0042] In the step (b), the grooved bead cores that can accommodate different numbers of cell flakes are made of polypropylene plastic.

[0043] The size of the bead core in step (b) is adjusted according to the length of the pearl oyster. Since the bead core has an irregular shape, the maximum length of the bead core is defined as the length (X) of the bead core, and the maximum length perpendicular to the length is defined as the width (Y). Therefore, the functional relationship between the length and width of the bead core and the length (W) of the pearl oyster shell is:

[0044]

[0045] The size of the bead core groove in step (c) is determined according to the size of the bead core and the number of grooves, and the groove length (γ) is: The groove width is calculated as a function of:

[0046]

[0047] Calculation is performed and the number of grooves (z) is:

[0048]

[0049] Wherein, the groove width is ω, the bead core length is X, and the bead core width is Y, and the unit is mm.

[0050] In order to overcome the sliding problem of cell flakes, the cell flakes are just filled into the groove of the bead nucleus to fix their position in the pearl oyster and quickly form high-quality zebra-patterned pearl sacs.

[0051] In step (d), the cultivation of zebra-patterned pearls is carried out by controlling the secretion ratio and arrangement of two specific colors of nacre in the pearl sac according to the size and position of the cell flakes, so as to achieve the color effect required by the market.

[0052] In step (f), a square-shaped pearl nucleus is implanted. The tip of a crochet hook is used to hook the epidermis of the inner membrane of the pearl oyster from right to left and make a small opening of about 12 mm in length. One side serving as a fulcrum is placed at the top and the other side is placed at the bottom and the pearl nucleus is slowly placed into the small opening.

[0053] During the breeding and cultivation process, the top of the pearl oyster shell and the sail are placed under the net bag, and the surgical wound is placed upwards. In the daily pearl breeding management, trace mineral elements, astaxanthin and β-carotene are regularly supplemented to ensure the nutrition necessary for the color formation of the pearl sac of the pearl oyster, making the color brighter and clearer. The color characteristics and glossiness of the front and back of the square pearls are taken out to judge the quality of the zebra-patterned pearl sac.

[0054] This example combines the core insertion technology of Hyriopsis cumingii and is implemented in the following four steps:

[0055] (1) Selection of clams and pearl oysters

[0056] One-year-old clams of the white strain of Hyriopsis cumingii "Shenzhe No. 3" and the purple strain of Hyriopsis cumingii "Shenzi No. 1" cultured in the same aquaculture water body were selected as the supply clams, and two-year-old white Hyriopsis cumingii in the same aquaculture water body were selected as the pearl-breeding clams.

[0057] (2) Zebra-patterned pearl shaping and cultivation

[0058] The two cell flakes provided by the clams are cut into long strips (10mm long × 3mm wide) and attached to the two grooves of the square bead nucleus (the bead nucleus is 15mm long, 12mm wide, and 1.2mm high, and the groove is a rectangular parallelepiped with a length of 10mm, a width of 3mm, and a height of 0.9mm). The grooves of the square bead nucleus correspond to the length and width of the cell flakes, respectively, and there is a 2mm gap between the two cell flakes. When implanting the square bead nucleus, use the tip of the crochet hook to hook the epidermis of the inner membrane of the pearl oyster from right to left and make a small hole about 12mm long. Place one side as the fulcrum at the top and the other side at the bottom and slowly put it into the small hole to reduce damage to the pearl oyster. After the pearl oyster is cultured for 4-6 months, zebra-striped pearl sacs are cultivated.

[0059] (3) Large-scale bead nucleus replacement and pearl cultivation

[0060] After the zebra pearl sac is complete, a small incision approximately 12mm long is made in the inner membrane of the pearl oyster to remove the square pearl nucleus. Select the pearl oyster that has produced square pearls with clear color, distinct lines, and high gloss on both sides. Continue inserting a 10mm diameter round bead nucleus. Then, place the round bead nucleus with small cell pieces attached into the pearl sac of the pearl oyster that has already removed the square pearl. Place the oyster in the water for pearl cultivation.

[0061] (4) Breeding management

[0062] Place the top and sail of the pearl oyster shell under the net bag, with the surgical wound facing upwards, to prevent the pearl nucleus from falling out. Follow routine freshwater pearl cultivation practices and regularly supplement with trace minerals, astaxanthin, beta-carotene, and other nutrients to ensure the necessary nutrients for the formation of the bicolor pearl's color, resulting in a brighter and more distinct zebra pattern.

[0063] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for cultivating zebra-patterned pearls, characterized in that: The steps include: (a) Directed cultivation of Hyriopsis cumingii donor oysters with different inner shell colors to obtain cell fragments that cause the pearl oysters to secrete nacre of a specific color; (b) shaping the grooves in the bead core to accommodate different numbers of cell sheets; (c) placing two cell fragments that control the secretion of nacre of a specific color by the pearl oyster in a predetermined arrangement in the groove of the bead nucleus, and inserting the bead nucleus into the mantle of the pearl oyster; (d) quickly prefabricating a pearl capsule capable of cultivating zebra-patterned pearls, thereby achieving the cultivation of zebra-patterned pearls; (e) After the incubation is completed, the pearls are removed and the quality of the zebra-patterned pearls is judged based on the pearl size, the color of the front and back surfaces, the distinctness of the zebra pattern, and the glossiness; (f) After the pearls are taken out, the pearl nucleus is replaced to produce zebra-patterned pearls for the second time; The size of the bead nucleus in step (b) is adjusted according to the length of the pearl oyster. Since the bead nucleus is irregular in shape, the maximum length of the bead nucleus is defined as the length X of the bead nucleus, and the maximum length perpendicular to the length is defined as the width Y. Therefore, the functional relationship between the length and width of the bead nucleus and the length W of the pearl oyster shell is:

2. The method for cultivating zebra-patterned pearls according to claim 1, wherein: The clams for supplying pearls and the clams for cultivating pearls in the step (a) are both Hyriopsis cumingii.

3. The method for cultivating zebra-patterned pearls according to claim 1, wherein: In the step (a), the directional cultivation of cuming clams with different inner shell colors is carried out by obtaining stable genetic clams through family selection and whole genome selection breeding, and the clams are directional cultivated by controlling the breeding environment and the conditions for feeding algae.

4. The method for cultivating zebra-patterned pearls according to claim 1, wherein: In the step (b), the bead core grooves that can accommodate different numbers of cell pieces are made of polypropylene plastic.

5. The method for cultivating zebra-patterned pearls according to claim 1, wherein: The size of the bead core groove in step (c) is determined according to the size of the bead core and the number of grooves, and the groove length (γ) is: The groove width is calculated as a function of: Calculation is performed and the number of grooves (z) is: Wherein, the groove width is ω, the bead core length is X, the bead core width is Y, γ is the groove length, and the unit is mm; z is the number of grooves; α is a constant.

6. The method for cultivating zebra-patterned pearls according to claim 1, wherein: In order to overcome the sliding problem of cell flakes, the cell flakes are just filled into the groove of the bead nucleus to fix their position in the pearl oyster and quickly form high-quality zebra-patterned pearl sacs.

7. The method for cultivating zebra-patterned pearls according to claim 1, wherein: The cultivation of zebra-patterned pearls in step (d) is to control the secretion ratio and arrangement of two specific colors of nacre in the pearl sac according to the size and position of the cell flakes, so as to achieve the color effect required by the market.

8. The method for cultivating zebra-patterned pearls according to claim 1, wherein: In the step (f), the square flake bead nucleus is hooked from right to left at the endometrium of the pearl oyster using the tip of a crochet hook and a small opening of 12 mm in length is made, and one side serving as a fulcrum is placed upward and the other side is placed downward and slowly placed into the small opening.

9. The method for cultivating zebra-patterned pearls according to claim 1, wherein: During the breeding and cultivation process, the top of the pearl oyster shell and the sail are placed under the net bag, and the surgical wound is placed upwards. Daily pearl breeding management regularly supplements trace mineral elements, astaxanthin, and β-carotene to ensure the nutrients necessary for the color formation of the pearl oyster pearl sac, making the color brighter and clearer. The quality of the zebra-patterned pearl sac is judged by testing the color characteristics and glossiness of the front and back of the square pearls.

Citation Information

Patent Citations

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