Modular bioprocessing plant

Abstract

This invention discloses a modular bioculture workshop, comprising a central control system and several independently configured bioculture systems. Each bioculture system includes at least one bioreactor, a central support unit, and a sub-control system. The central support unit has several main pipelines and longitudinal pipelines. The main pipelines are connected to branch pipelines, which are directly or indirectly connected to the bioreactors. The ends of the main pipelines are equipped with sealing devices and independent pipe connectors. By integrating the main pipelines, longitudinal pipelines, and branch pipelines onto the central support unit, and by providing sealing devices and pipe connectors at the ends of the main pipelines, and by establishing communicative connections between the central and sub-control systems, the problems of complex piping, difficult installation, and maintenance in bioculture systems are solved. Furthermore, it enables material transfer between different bioculture systems, preventing economic losses caused by prolonged system malfunctions leading to unsuitable cultured organisms for production.

Description

Technical Field

[0001] This invention belongs to the technical field of plant tissue culture devices, specifically, it relates to a modular biological culture workshop. Background Technology

[0002] In recent years, biological tissue and cell culture technology has been widely used in the production of various medical products, cosmetics, health products and beverages. The market prospects for plant tissues and cells cultured by this technology and their derivative products are very broad, and the demand is increasing year by year as people's living standards continue to improve.

[0003] However, the culture of plant tissues and cells requires relatively harsh conditions, which are often difficult to achieve for large-scale plant tissue and cell cultures. Taking the culture of adventitious roots as an example, the biological culture system for cultivating adventitious roots requires a large number of pipes to regulate and maintain the culture environment inside the bioreactor. The large number and complex arrangement of these pipes increase the difficulty for technicians to install, operate, and maintain them. Furthermore, it is difficult to achieve effective material transfer between different biological culture systems. Consequently, when one biological culture system malfunctions, other normally operating biological culture systems cannot connect to the pipes of the malfunctioning system. Under such circumstances, it is difficult to avoid the bioreactor of the malfunctioning biological culture system producing plant adventitious roots of poor quality.

[0004] In view of this, the present invention is proposed. Summary of the Invention

[0005] The purpose of this invention is to provide a modular bioculture workshop. By integrating the main pipelines and longitudinal pipelines of several independently set bioculture systems on the back of a main support device, and extending the branch pipelines connected to the main pipelines through the main support device toward the bioreactor and directly or indirectly connected to the bioreactor, and providing detachable sealing devices at the ends of the main pipelines, and setting independent pipeline connectors between two bioculture systems, this invention not only solves the problems of complex pipelines in bioculture systems, making installation and maintenance difficult, but also realizes the transfer of materials between different bioculture systems. This avoids economic losses caused by the cultured biological tissues or cells failing to meet production requirements due to prolonged failures of the bioculture systems.

[0006] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is as follows:

[0007] A modular bioculture workshop includes several independently configured bioculture systems and a central control system. Each bioculture system comprises: at least one bioreactor, a central support device located on one side of the bioreactor, and a sub-control system located inside the central support device. The workshop is characterized by...

[0008] The back of the main support device has several main pipelines arranged horizontally and several longitudinal pipelines arranged vertically, each connected to the main pipelines.

[0009] The main pipeline is connected to branch pipelines, which pass through the main support device and extend toward the bioreactor and are directly or indirectly connected to the bioreactor.

[0010] The main pipeline is provided with a detachable sealing device at at least one end;

[0011] A central control system communicates with each sub-control system.

[0012] Furthermore, the modular biological culture workshop also includes independent pipeline connectors. After the sealing device is disassembled, the pipeline connectors can connect the main pipeline (B1) of the biological culture system that has malfunctioned to another normal main pipeline in the corresponding biological culture system. This is to ensure that material transfer can be carried out between different biological culture systems when the main control system and / or the sub-control system displays a fault in the main pipeline and / or the longitudinal pipeline.

[0013] Furthermore, several independently configured biological culture systems are spaced apart, and the installation position of the main pipeline of the biological culture system corresponds to the installation position of the main pipeline of another adjacent biological culture system.

[0014] Furthermore, the main pipeline is evenly distributed vertically on the main support device, the longitudinal pipeline is concentrated horizontally on the main support device, and the connection between the longitudinal pipeline and the main pipeline is close to the middle of the main pipeline.

[0015] Furthermore, the line connecting the ends of the main pipeline is perpendicular to the horizontal plane, and the position of the ends of the main pipeline does not exceed the edge of the main support device.

[0016] Furthermore, the main pipeline includes:

[0017] The main gas supply pipe is used to introduce culture gas into the interior of the bioreactor;

[0018] Steam supply main pipe, used to introduce steam into the interior of the bioreactor and auxiliary devices or the temperature control layer of the bioreactor;

[0019] The liquid supply main pipe is used to introduce liquid into the temperature control layer of the bioreactor;

[0020] The liquid recovery main pipe is used to recover the liquid introduced into the temperature control layer of the bioreactor;

[0021] The pipeline is provided in several parts. One end of each pipeline is connected to at least one of the four main pipelines: gas supply main, steam supply main, liquid supply main, and liquid recovery main. The other end of each pipeline is directly or through a pipeline connected to the bioreactor. The opening and closing of the pipeline is controlled by the corresponding pipeline valve to transport the medium.

[0022] Furthermore, the sub-control system is connected to the branch valve, and the sub-control system can control the opening and closing of the branch valve to regulate the introduction rate and duration of culture gas, steam and liquid into the bioreactor.

[0023] The biological culture system is equipped with a temperature control system, which includes at least a temperature control water tank. The inlet of the temperature control water tank is connected to the liquid recovery main pipe, and the outlet of the temperature control water tank is connected to the liquid supply main pipe, so that the medium circulates between the bioreactor and the temperature control water tank.

[0024] Furthermore, the sub-control system monitors the amount of medium and / or the temperature of the medium entering the biological culture system and the culture parameter data of the bioreactor in real time. When the data detected by the sub-control system differs from the preset value and the duration reaches the preset time, the sub-control system issues an early warning message; the data of the sub-control system is transmitted to the main control system in real time.

[0025] Furthermore, the main control system is connected to the pipeline connector; when the sub-control system issues an early warning message, the main control system controls the pipeline connector to connect the faulty main pipeline in the biological culture system to the corresponding normal main pipeline in the biological culture system; when the faulty main pipeline is repaired and resumes normal operation, the data detected by the sub-control system is the same as the preset value, and the duration reaches the preset time, the main control system controls the pipeline connector to disconnect the two main pipelines.

[0026] Furthermore, the main support device includes an adjacent main support frame and a second support frame. The main support frame and the second support frame are interconnected by a number of horizontal bars, vertical bars and uprights to form a hollow support frame. The main support frame and the second support frame are adjacent independent frame structures, or frame structures that share horizontal bars, vertical bars and uprights. The main pipeline, branch pipelines and longitudinal pipelines are fixed on the main support device.

[0027] By adopting the above technical solution, the present invention has achieved the following beneficial effects.

[0028] 1. By integrating the main pipelines and longitudinal pipelines of several independently configured biological culture systems on the back of the main support device, and extending the branch pipelines connected to the main pipelines through the main support device toward the bioreactor and directly or indirectly connecting them to the bioreactor, the problem of complex pipelines in the biological culture system, which are difficult to install and maintain, is solved.

[0029] 2. By installing a detachable sealing device at the end of the main pipeline, and then connecting the main pipeline of the malfunctioning biological culture system to the corresponding main pipeline of another normally functioning biological culture system using pipeline connectors, not only are both ends of the main pipeline sealed to prevent media leakage, but also material transfer between different biological culture systems is achieved. This prevents the plant tissues or cells cultured by the malfunctioning biological culture system from failing to meet production requirements and causing economic losses. Attached Figure Description

[0030] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0031] Figure 1 This is a schematic diagram of the layout of a modular biological culture workshop in an embodiment of the present invention;

[0032] Figure 2 This is a three-dimensional schematic diagram of the tubing fixing frame of the biological culture system in an embodiment of the present invention;

[0033] Figure 3 This is a front view of the tubing fixture of the biological culture system in an embodiment of the present invention;

[0034] Figure 4 This is a top view of the tubing fixture of the biological culture system in an embodiment of the present invention;

[0035] Figure 5 This is a schematic diagram of the piping structure of the biological culture system in an embodiment of the present invention;

[0036] Figure 6 This is a schematic diagram of the piping structure including the temperature control system in an embodiment of the present invention;

[0037] Figure 7 This is a schematic diagram of the piping structure of the temperature control system of the biological culture system in an embodiment of the present invention;

[0038] Figure 8 yes Figure 6 A schematic diagram of the connection of the piping in area A within the overall support frame of the temperature control system.

[0039] Figure 9 yes Figure 8 A schematic diagram showing the connection between the intake structure and the intake pipeline in Zone B.

[0040] Among them: 1. Outer horizontal bar; 2. Inner horizontal bar; 3. Outer vertical bar; 4. Inner vertical bar; 5. Inner longitudinal bar; 6. Outer longitudinal bar; 7. Upper support longitudinal bar; 8. Lower support longitudinal bar; 9. Inner support vertical bar; 10. Outer support vertical bar; 11. Second outer horizontal bar; 12. Third outer horizontal bar; 21. Second inner horizontal bar; 211. Second outer support horizontal bar; 22. Third inner horizontal bar; 31. Second outer vertical bar; 32. Third outer vertical bar; 41. Second inner vertical bar; 42. Third inner vertical bar; 51. Second inner longitudinal bar; 52. Third inner longitudinal bar; 61. Second outer longitudinal bar; 611. Second inner support longitudinal bar; 62. Third outer longitudinal bar; 71. Second outer support longitudinal bar; 91. Second inner support vertical bar; 101. Second outer support vertical bar; 100. Seed Tank; 200, Culture tank; 300, Fixing device; 400, Mounting device; 410, Pressure reducing filter; 420, Discharge branch; A1, Supply unit; A2, Conveying unit; A3, Temperature-controlled water tank; A21, Inlet pipe; A22, Outlet pipe; A31, Temperature control layer; A23, Heat exchange structure; A24, Liquid inlet pipe; A25, Liquid outlet pipe; A110, Air inlet branch; A120, Check valve; A130, Filter device; A140, Filter disinfection branch; A150, Tank disinfection branch; A160, Backup branch; A170, Exhaust pipe; A180, Gas filter; A211, Water pump; A212, Branch pipe; A241, Chilled water inlet pipe; A242, Steam inlet pipe; A251. Chilled water outlet pipe; A252, Steam outlet pipe; A301, Water container; A1101, First air inlet branch; A1102, Second air inlet branch; A1103, Third air inlet branch; A1104, Fourth air inlet branch; A1105, Fifth air inlet branch; A1106, Air inlet branch pipe; A1107, Air inlet branch pipe; C20, Chilled water supply branch pipe; D20, Cooling water supply branch pipe; E20, Hot water supply branch pipe; C10, Chilled water recovery branch pipe; D10, Cooling water recovery branch pipe; E10, Hot water recovery branch pipe; B10, Steam supply branch pipe; A100, Main steam supply pipe; B100, Main steam supply pipe; C100, Main chilled water recovery pipe; D100, Main cooling water recovery pipe; E 100. Hot water recovery main pipe; C200. Chilled water supply main pipe; E200. Hot water supply main pipe; D200. Cooling water supply main pipe; C300. Cold radiator pipe; C400. Hot radiator pipe; B1. Main pipe; B2. Branch pipe; C1. Longitudinal pipe; B3. Cascade culture device; B4. Pneumatic valve; B5. Manual valve; B6. Input pipe; B7. Output pipe; B8. Air inlet tee; B40. Air inlet structure; B41. Air inlet section; X100. Biological culture system; X101. Main support device; X102. Bioreactor; X1011. Main support frame; X1012. Secondary support frame; Y1. Pipe connector; X11. Liquid supply main pipe; X12. Liquid recovery main pipe.

[0041] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0043] In the description of this invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "inner", "outer", "lower", "higher", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0044] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0045] This invention provides a modular biological culture workshop, such as Figure 1-4 As shown in one embodiment, the modular bioculture workshop of the present invention includes several independently configured bioculture systems X100 and a main control system. Each bioculture system X100 includes: at least one bioreactor X102, a main support device X101 disposed on one side of the bioreactor X102, and a sub-control system disposed inside the main support device X101. The back of the main support device X101 is provided with several main pipelines B1 arranged horizontally and several longitudinal pipelines C1 arranged vertically and respectively connected to the main pipelines B1. The main pipelines B1 are connected to branch pipelines B2, which extend through the main support device X101 toward the bioreactor X102 and are directly or indirectly connected to the bioreactor X102.

[0046] The main pipeline B1 is provided with a detachable sealing device at at least one end. The sealing device seals the end of the main pipeline B1 to prevent the culture air, steam or liquid or other media inside the main pipeline B1 from leaking from the end of the main pipeline B1 and affecting the normal operation of the bioreactor X102.

[0047] A central control system is communicatively connected to each sub-control system. Each sub-control system detects and records the culture parameter data of the biological culture system X100 to which it is located, as well as the amount of medium and / or the temperature of the medium entering the biological culture system X100, and uploads the data to the central control system. When the data detected by the sub-control system is different from the preset value, the sub-control system issues an early warning message. At this time, the central control system analyzes the current data and issues corresponding control commands based on the analysis results.

[0048] The modular biological culture workshop of the present invention also includes an independent pipeline connector Y1. The pipeline connector Y1 can transmit media such as air, steam or liquid. After the sealing device is disassembled, the pipeline connector Y1 can connect the main pipeline B1 of the biological culture system X100 that has failed to function properly to the corresponding main pipeline B1 of another biological culture system X100. This is to ensure that material transfer can be carried out between different biological culture systems X100 when the main control system and / or the sub-control system displays a failure in the main pipeline B1 and / or the longitudinal pipeline C1.

[0049] When a malfunction occurs in a biological culture system X100 where the culture medium (air, steam, or liquid) in the main pipeline B1 fails to meet the operating conditions of the bioreactor X102, it is necessary to temporarily replace the medium in the main pipeline B1. This allows the bioreactor X102 to be promptly supplied with the appropriate medium. The sealing device is then removed from the malfunctioning end of the main pipeline B1. One end of the pipe connector Y1 is connected to one end of the main pipeline B1, and the other end of the pipe connector Y1 is connected to the corresponding main pipeline B1 of another normally functioning biological culture system X100. The appropriate medium is then introduced into the main pipeline B1 of the malfunctioning biological culture system X100, ensuring that the bioreactor X102 is promptly supplied with the appropriate medium. This prevents the culture environment in the bioreactor X102 from being in an abnormal state for an extended period, which could lead to the cultured biological products failing to meet production conditions and causing economic losses.

[0050] Preferably, the sealing device has a connecting structure and a connecting switch. The connecting structure can be directly connected to the pipeline connector Y1 without disassembling the sealing device. After the connection is completed, the connecting switch of the sealing device is adjusted to connect the main pipeline B1 to the pipeline connector Y1, so as to quickly connect the main pipeline B1 to the pipeline connector Y1, save time, and avoid leakage of the medium in the main pipeline B1 during the disassembly of the sealing device.

[0051] The pipeline connector Y1 has two working states, including:

[0052] In the first working state, the main pipeline B1 of the biological culture system X100 is connected to the corresponding main pipeline B1 of another biological culture system X100.

[0053] In the second operating state, the connection between the main pipeline B1 of the biological culture system X100 and the corresponding main pipeline B1 of the other biological culture system X100 is blocked.

[0054] In the above scheme, the two working states are switched by setting an on / off switch on the pipe connector Y1 to control the flow of the medium inside the pipe connector Y1. When the on / off switch is open, the pipe connector Y1 switches to the first working state, and the medium can be transferred through the inside of the pipe connector Y1 from the main pipeline B1 of a normally functioning biological culture system X100 to the main pipeline B1 of another biological culture system X100 that has malfunctioned. When the on / off switch is closed, the pipe connector Y1 switches to the second working state, and the medium cannot be transferred through the pipe connector Y1 to the main pipeline B1 of another biological culture system X100.

[0055] The biological culture systems X100 are set up independently and spaced apart. Each biological culture system X100 does not affect the others. When one biological culture system X100 fails, the other biological culture systems X100 can still work normally. The spacing also provides space for the staff to install, operate and maintain the system.

[0056] like Figure 1 As shown, the installation position of the main pipeline B1 of the biological culture system X100 corresponds to the installation position of the main pipeline B1 of the adjacent biological culture system X100. The extension direction of the main pipeline B1 of the biological culture system X100 is the same as the extension direction of the main pipeline B1 of the other biological culture system X100, and the extension directions of the main pipeline B1 of the biological culture systems X100 in the same row are on the same straight line, so as to facilitate the connection of the pipeline connector Y1 to the main pipeline B1 of the two biological culture systems X100.

[0057] like Figure 1As shown, in this embodiment, the main support devices X101 of several biological culture systems X100 are arranged opposite each other in a straight line. The main pipeline B1 and the longitudinal pipeline C1 of the biological culture system X100 are close to each other, and space is left between the main support devices X101 of the biological culture system X100 to facilitate the connection and use of the pipeline connector Y1.

[0058] like Figure 1 As shown, the two biological culture systems X100 connected by the pipe connector Y1 can be adjacent to each other on the left and right, front and back, or diagonally, so as to connect the two biological culture systems X100 as needed according to the actual production situation.

[0059] The main pipelines B1 are evenly distributed vertically on the main support device X101 to facilitate the differentiation of different main pipelines B1 with different types of media, and to facilitate the connection of the longitudinal pipelines C1 and the branch pipelines B2 to the corresponding main pipelines B1.

[0060] The longitudinal pipeline C1 is centrally distributed horizontally on the main support device X101. The longitudinal pipeline C1 connects to the upper floor of the current workshop. The upper floor has a large number of equipment that produce the medium that the longitudinal pipeline C1 needs to pass through. The upper floor has a large load-bearing capacity. The centralized distribution of the longitudinal pipeline C1 helps to avoid the weighing structure buried on the floor, reduce damage to the building structure, and save space.

[0061] The longitudinal pipe C1 extends perpendicular to the horizontal plane, and the connection between the longitudinal pipe C1 and the main pipe B1 is close to the middle of the main pipe B1. This arrangement can reduce the transmission distance of the medium in the longitudinal pipe C1 from the longitudinal pipe C1 through the main pipe B1 and the branch pipe B2 to finally reach the bioreactor X102, thereby improving the transmission efficiency of the medium and reducing the energy loss of the medium during the transmission process.

[0062] The line connecting the ends of the main pipeline B1 is perpendicular to the horizontal plane, and the position of the end of the main pipeline B1 does not exceed the edge of the main support device X101, so that the staff can distinguish the main pipeline B1 that carries different media, which is beneficial to the installation of the pipeline connector Y1, and improves the working environment of the staff, preventing the staff from accidentally bumping into the end of the main pipeline B1 while working, and ensuring the safety of the staff.

[0063] In this embodiment, the main pipeline B1 includes:

[0064] The gas supply main pipe A100 is used to introduce culture gas into the interior of the bioreactor X102;

[0065] Steam supply main pipe B100 is used to supply steam to the interior of bioreactor X102 and auxiliary devices or the temperature control layer A31 of bioreactor X102.

[0066] The liquid supply main pipe X11 is used to introduce liquid into the temperature control layer A31 of the bioreactor X102;

[0067] The liquid recovery main pipe X12 is used to recover the liquid introduced into the temperature control layer A31 of the bioreactor X102;

[0068] When the bioreactor X102 requires high-temperature sterilization, the steam supply main pipe B100 introduces high-temperature steam into the interior of the bioreactor X102 or into the temperature control layer A31 of the bioreactor X102. The auxiliary device includes a pipeline connecting to the interior of the bioreactor X102 for transferring plant tissue and / or materials required for culturing plant tissue, a valve for controlling the opening and closing of the pipeline, and a steam hose connecting to the valve. When the bioreactor X102 needs to transfer plant tissue and / or materials required for culturing plant tissue through the auxiliary device, the steam supply main pipe B100 introduces high-temperature steam into the auxiliary device to sterilize the parts of the auxiliary device that come into contact with the transferred plant tissue and / or materials required for culturing plant tissue at high temperature, so as to avoid contamination of the plant tissue and / or materials required for culturing plant tissue after passing through the auxiliary device.

[0069] The liquid supply main pipe X11 includes a chilled water supply main pipe C200, a cooling water supply main pipe D200, and a hot water supply main pipe E200. The chilled water supply main pipe C200 is used to introduce chilled water into the temperature control layer A31 of the bioreactor X102 to maintain the internal temperature of the bioreactor X102 at a preset temperature. The cooling water supply main pipe D200 is used to introduce cooling water into the temperature control layer A31 of the bioreactor X102 to cool down the internal temperature of the bioreactor X102 until it returns to the preset temperature when the internal temperature is too high. The hot water supply main pipe E200 is used to introduce hot water into the temperature control layer A31 of the bioreactor X102 to heat up the internal temperature of the bioreactor X102 until it returns to the preset temperature when the internal temperature is too low.

[0070] The liquid recovery main pipe X12 includes a chilled water recovery main pipe C100, a cooling water recovery main pipe D100, and a hot water recovery main pipe E100. The chilled water recovery main pipe C100 is used to recover chilled water introduced into the temperature control layer A31 of the bioreactor X102; the cooling water recovery main pipe D100 is used to recover cooling water introduced into the temperature control layer A31 of the bioreactor X102; and the hot water recovery main pipe E100 is used to recover hot water introduced into the temperature control layer A31 of the bioreactor X102.

[0071] By recycling and reusing chilled water, cooling water, and hot water, resource waste can be reduced and the operating costs of the X100 biological culture system can be saved.

[0072] The branch pipes B2 are provided in several parts. One end of each branch pipe B2 is connected to at least one of the four main pipes: gas supply main pipe A100, steam supply main pipe B100, liquid supply main pipe X11, and liquid recovery main pipe X12. The other end of each branch pipe B2 is directly or through a pipe connected to the bioreactor X102. The opening and closing of the branch pipe B2 is regulated by the corresponding branch valve to transport the medium, so as to transfer the medium in the above four pipes to the bioreactor X102 and maintain the normal operation of the bioreactor.

[0073] At least one branch valve corresponding to branch pipe B2 is provided on branch pipe B2. The number of branch valves is greater than or equal to the number of branch pipes B2. The branch valve can regulate the on / off state of the branch pipe B2. When the branch valve is open, the branch pipe B2 where the branch valve is located can transfer the medium from the main pipe B1 to the temperature control layer A31 or the interior of the bioreactor X102. When the branch valve is closed, the branch valve prevents the medium from the main pipe B1 from being transferred to the temperature control layer A31 or the interior of the bioreactor X102 through the branch pipe B2 where it is located.

[0074] The sub-control system is connected to the branch valve, and the sub-control system can control the opening and closing of the branch valve to regulate the flow rate and duration of culture gas, steam and liquid media introduced into bioreactor X102.

[0075] The sub-control system monitors the amount of medium and / or temperature of the medium entering the biological culture system X100 and the culture parameter data of the bioreactor X102 in real time. When the data detected by the sub-control system is different from the preset value and the duration reaches the preset time, the sub-control system issues an early warning message. The data of the sub-control system is transmitted to the main control system in real time so that the main control system can analyze the current data and issue control commands based on the analysis results.

[0076] The main control system is connected to the pipeline connector Y1. When the sub-control system issues an early warning message, the main control system controls the pipeline connector Y1 to connect the faulty main pipeline B1 in the biological culture system X100 to the corresponding normal main pipeline B1 in the other biological culture system X100. When the faulty main pipeline B1 is repaired and resumes normal operation, the data detected by the sub-control system is the same as the preset value, and the duration reaches the preset time, the main control system controls the pipeline connector Y1 to disconnect the two main pipelines B1.

[0077] In the above scheme, the warning information issued by the sub-control system includes text information. The sub-control system will display the collected data on the amount of medium and / or the temperature of the medium entering the biological culture system X100 and the culture parameter data of the bioreactor X102 in real time on the control panel of the biological culture system X100. At the same time, the control panel will also display the current preset amount of medium and / or the preset temperature of the medium entering the biological culture system X100 and the preset culture parameter data of the bioreactor X102, so as to facilitate comparison and processing by the staff.

[0078] The medium introduced into the biological culture system X100 includes at least steam, cooling water, chilled water, culture air, and compressed air; the culture parameter data of the bioreactor X102 includes at least the amount of culture air in the culture medium, the pH of the culture medium, the nutrient content, the amount of foam generated in the culture medium, the temperature of the culture medium, and the pressure inside the bioreactor X102.

[0079] The preset medium volume and / or preset medium temperature data displayed on the control panel, as well as the preset culture parameter data of the bioreactor X102, are all range values. However, the medium volume and / or medium temperature data introduced into the bioreactor X100, and the culture parameter data of the bioreactor X102, are all specific numerical values. When the value is within the range, the sub-control system assumes that the bioreactor X100 is working normally. When the value is outside the range, the sub-control system determines that the bioreactor X100 has malfunctioned, issues a warning message, and prompts the operator to connect the corresponding pipeline connector Y1. This connects the main pipeline B1 of the normally functioning bioreactor X100 system to the corresponding main pipeline B1 of the malfunctioning bioreactor X100 system. The main control system is also connected to the on / off switch of the pipeline connector Y1, so that the main control system can control the opening and closing of the on / off switch, thereby switching the working state of the pipeline connector Y1 to the first working state, introducing a medium that meets the working requirements of the bioreactor X102, replacing the original medium that does not meet the working requirements, and maintaining the normal operation of the bioreactor X102.

[0080] Pipeline connector Y1 can also be pre-connected to the corresponding main pipeline B1 of the two biological culture systems X100 before the sub-control system detects a fault in the biological culture system X100. The main control system connects to the on / off switch of pipeline connector Y1 so that when the sub-control system detects a fault in the biological culture system X100, the main control system can immediately control the on / off switch to open, switch the working state of pipeline connector Y1 to the first working state, and introduce the medium that meets the working requirements of bioreactor X102. This reduces the labor intensity of the staff and reduces the time that the biological culture system X100 is in fault condition, keeping the culture state inside bioreactor X102 basically unchanged.

[0081] When the malfunction in the biological culture system X100 is repaired by the staff, and the sub-control system detects that the data of the medium transmitted from the longitudinal pipeline C1 in the main pipeline B1 meets the working requirements of the bioreactor X100, the main control system controls the opening and closing, switches the working state of the pipeline connector Y1 to the second working state, and the sealing device re-closes the end of the main pipeline B1.

[0082] When the media from the longitudinal pipeline C1 and the temperature-controlled water tank A3 in the liquid supply main pipe X11 do not meet the cultivation conditions of the bioreactor X102, the liquid supply main pipe X11 of the currently malfunctioning bioreactor X100 needs to be connected to the liquid supply main pipe X11 of another normally functioning bioreactor X100. Furthermore, depending on the actual situation, it should be determined whether the liquid recovery main pipe X12 of the currently malfunctioning bioreactor X100 needs to be connected to the corresponding normally functioning liquid recovery main pipe X12 of another bioreactor X100. If the liquid recovery main pipe X12 of the malfunctioning bioreactor X100 cannot properly recover the media from the liquid supply main pipe X11 of another normally functioning bioreactor X100, then it needs to be connected to the corresponding normally functioning liquid recovery main pipe X12 of the bioreactor X100 so that the bioreactor X102 in the malfunctioning bioreactor X100 can maintain normal operation. Depending on the actual situation, the supply and discharge of media such as steam and cultivation air can also be selected with reference to the above methods.

[0083] The main support device X101 includes an adjacent main support frame X1011 and a second support frame X1012. The main support frame X1011 and the second support frame X1012 are connected by a number of horizontal bars, vertical bars and uprights to form a hollow support frame. The main support frame X1011 and the second support frame X1012 are adjacent independent frame structures, or frame structures that share horizontal bars, vertical bars and uprights. The main pipeline B1, the branch pipeline B2 and the longitudinal pipeline C1 are fixed on the main support device X101.

[0084] When the individual biological culture system X100 needs to be moved, the operator can disconnect the branch pipe B2 from the bioreactor X102 and disconnect the longitudinal pipe C1 from the equipment on the upper floor. The biological culture system X100 is then divided into two parts: one part is an integrated unit composed of the main support device X101, the main pipe B1, the longitudinal pipe C1, and the branch pipe B2; the other part is the bioreactor X102 and its support components. This reduces the volume during handling and distributes the overall weight of the biological culture system X100, making it easier for operators or engineering equipment to move the biological culture system X100.

[0085] like Figure 5-7 As shown in the embodiment of the present invention, a pipeline structure of a cascade culture device B3 is described. In order to reduce the number of times the cultured plant tissues are inoculated into the cascade culture device B3 and to achieve the purpose of multiple cycles of proliferation of plant tissues, the cascade culture device B3 is divided into a seed tank 100 and a culture tank 200 according to the tanks used for culturing plant tissues or cells.

[0086] The culture tank 200 is used for the rapid cultivation and propagation of plant tissues, enabling the mass production of plants. The seed tank 100 is used to incubate plant tissues, serving as seeds for the rapid cultivation and propagation of plant tissues in the next cycle of the culture tank 200.

[0087] In particular, the seed tank 100 is smaller in volume than the culture tank 200, being 1 / 3 to 1 / 5 the volume of the culture tank 200.

[0088] In actual production, the process of transferring and replanting is as follows: after all the culture medium and plant tissue in the seed tank 100 are introduced into the culture tank 200, the culture medium in the culture tank 200 is first raised to the set water level to dilute the concentration of the plant tissue, and then the diluted plant tissue and culture medium mixture is immediately transported into the seed tank 100.

[0089] Specifically, the cascade culture device B3 is provided with at least one seed tank 100 and one culture tank 200, which are connected in parallel to the main pipeline B1 via the branch pipeline B2. Furthermore, a seed delivery pipe and a reverse seeding pipe are connected between the seed tank 100 and the culture tank 200.

[0090] The seed delivery tube is used to transfer all the culture medium and plant tissue in the seed tank 100 into the culture tank 200; the reverse seed tube is used to transfer the diluted culture medium and plant tissue in the culture tank 200 back to the seed tank 100.

[0091] In this embodiment, by setting the cascade culture device B3 as a seed tank 100 and a culture tank 200, the number of times the cascade culture device B3 is inoculated is reduced, and the purpose of multiple cyclic proliferation of plant tissue is achieved, which greatly improves the efficiency of large-scale production of plant tissue.

[0092] In this embodiment of the invention, the front of the main support frame X1011 is further provided with a bioreactor fixing frame, wherein the bioreactor X102 is a single culture device or a cascaded culture device B3, and the bioreactor X102 includes at least a seed tank 100, which is mounted on the bioreactor fixing frame. The bioreactor fixing frame includes two parallel third inner uprights 42, and the two ends of the third inner uprights 42 are perpendicularly connected to two parallel third inner crossbars 22. The bioreactor fixing frame also includes two third outer uprights 32 parallel to the third inner uprights 42, and the bottom end of the third outer uprights 32 is connected to a third outer crossbar 12. The two ends of the third outer crossbar 12 are perpendicularly connected to the bottom ends of the two third outer uprights 32, respectively. The third outer crossbar 12 is parallel to the third inner crossbar 22, and adjacent third outer uprights 32 and third inner crossbars 22 are parallel to each other. The inner vertical rod 42 is connected to the third inner longitudinal rod 52 and the third outer longitudinal rod 62 at both ends. The two ends of the third inner longitudinal rod 52 are perpendicularly connected to the ends of the third outer vertical rod 32 and the third inner vertical rod 42, which are in the same vertical plane. The two ends of the third outer longitudinal rod 62 are perpendicularly connected to the ends of the third outer vertical rod 32 and the third inner vertical rod 42, which are in the same vertical plane. The third inner longitudinal rod 52 and the third outer longitudinal rod 62 at the height of the bioreactor mounting frame are respectively provided with a mounting device 400 for fixing the seed tank 100. The third outer vertical rod 32 and the third inner vertical rod 42 have a certain height so that the seed tank 100 can be suspended and installed on the bioreactor mounting frame. The third outer longitudinal rod 62 and the third inner longitudinal rod 52 have a certain length so that the center of gravity of the seed tank 100 is located within the space where the bioreactor mounting frame is located. A part of the seed tank 100 protrudes from the bioreactor mounting frame.

[0093] The plane formed by the connection of the third inner upright 42 and the third inner crossbar 22 is in contact with the front of the main support frame X1011. The third inner upright 42 is in contact with the inner upright 4 and the second inner upright 41. The lower third inner crossbar 22 is in contact with the inner crossbar 2.

[0094] In another embodiment, at the junction of the bioreactor fixing frame and the main support frame X1011, the third inner upright 42 and the inner upright 4 are combined to form the inner upright 4, the third inner crossbar 22 and the inner crossbar 2 are combined to form the inner crossbar 2, and the third inner longitudinal bar 52 is an extension of the inner longitudinal bar 5.

[0095] In this embodiment of the invention, a plurality of fixing devices 300 are evenly distributed within the main support frame X1011 and the second support frame X1012.

[0096] Specifically, a plurality of fixing devices 300 for fixing and installing pipeline structures are evenly distributed on the inner sides of the outer uprights 3, outer support uprights 10, and second outer uprights 31 on the main support frame X1011 and the second support frame X1012. A plurality of fixing devices 300 for fixing and installing pipeline structures are evenly distributed on the outer crossbar 1 on the back of the main support frame X1012 and between the two outer support uprights 10. The pipeline structure is distributed as follows: the main pipeline B1 fixed to the outer uprights 3, outer support uprights 10, and second outer uprights 31 is parallel to the outer crossbar 1 and extends along the direction of the outer crossbar 1. The main pipeline B1... The main pipeline B1 can extend from the right outer upright 3 to the second outer upright 31 along the direction of the outer horizontal bar 1, that is, the main pipeline B1 extends from the outer end of the main support frame X1011 to the outer end of the second support frame X1012; the longitudinal pipeline C1 fixed on the outer horizontal bar 1 is parallel to the outer upright 3 and extends along the direction of the outer upright 3. The longitudinal pipeline C1 extends upward to another space and downward to the corresponding main pipeline B1, and connects with the corresponding main pipeline B1 to provide the medium required by the culture device to the main pipeline B1. The longitudinal pipeline C1 can be concentrated in the middle area of ​​the main support frame X1011 to reduce the energy change when the medium is transmitted in the pipeline.

[0097] The present invention provides a total support device X101, which is further provided with multiple wire grooves for installing and fixing wires. The wire grooves are arranged along the outer horizontal bar 1 and / or outer vertical bar 3 and / or inner vertical bar 5 of the total support frame X1011. The wires are neatly placed into the wire grooves to avoid messy wire connections, which is beneficial to the maintenance and repair of the circuit, and prevents the wires from causing safety hazards to the operators due to leakage, aging, etc.

[0098] Specifically, the cable trays used for installing and fixing the wires are arranged as follows: the wires are installed in the cable trays, which are set along the direction of the outer horizontal bar 1 and / or outer vertical bar 3 and / or inner longitudinal bar 5 of the main support frame X1011. According to control needs, when the wires need to connect to equipment such as tanks and valves in the system, the wires are led out from one end of the cable trays and connected to the aforementioned equipment. The wires are also connected to the control cabinet in the first space from the other end of the cable trays, so that the control elements inside the control cabinet can control the tanks and valves in the system to work.

[0099] The main support frame X1011 and the second support frame X1012 of this invention can be connected by threaded connections, welding, or riveting, etc. In this embodiment, welding is preferred as the connection method for the horizontal bars, vertical bars, and vertical bars. Figures 2 to 4As shown, multiple fixing devices 300 are evenly arranged on the main support frame X1011 and the second support frame X1012. The fixing devices 300 can be connected to the main support frame X1011 and the second support frame X1012 by threaded connection, welding or riveting. In this embodiment, the fixing device 300 is preferably connected to the crossbar and the upright by welding. The top of the fixing device 300 is provided with a clamp for fixing the pipelines installed in the biological culture system X100. The various devices in the system are connected by pipelines. Culture medium, cooling water, chilled water, hot water, steam, culture air, compressed gas, etc. are introduced into the pipelines to provide the necessary substances for the operation of each device and to regulate the temperature required for the operation of each device, so as to realize the continuous operation of the entire biological culture system X100.

[0100] like Figure 5-7 The diagram shows the piping structure of the biological culture system X100 in this invention. Specifically, the piping structure includes a main pipe B1 and a branch pipe B2 connected to the main pipe B1, which are disposed on the back of the main support frame X1011 and the second support frame X1012. The main pipe B1 is separated from the cascade culture device B3.

[0101] In the embodiments of the present invention, the main pipeline B1 is also referred to as the main pipe B1, and the branch pipeline B2 is also referred to as the branch pipe B2. The pipeline structure includes several main pipes B1 and branch pipes B2 that connect the main pipes B1 with the bioreactor X102 and other required equipment. The pipeline structure is arranged as follows: each main pipe B1 is distributed parallel to the outer crossbar 1 and is fixedly installed on the main support frame X1011 by the fixing device 300. Each main pipe B1 can extend from the right outer upright 3 to the second outer upright 31 along the direction of the outer crossbar 1. The fixing device 300 of the pipeline is connected to the pipeline by bolts and / or clamps.

[0102] like Figure 5-9 As shown in the embodiment of the present invention, the gas supply system of the cascade culture device B3 is described. The gas supply system includes a main gas supply pipe A100 and a main steam supply pipe B100, with an inlet branch A110 connected to the main gas supply pipe A100. The main steam supply pipe B100 is provided with a steam supply branch pipe B10, which is connected to the inlet branch pipe A110 within the main support device X101.

[0103] To better decompose air into small bubbles that dissolve in the culture medium and increase the solubility of culture air in the culture medium, the gas supply system also includes an air intake structure B40 installed on the bottom side wall of the cascade culture device B3. One end of the air intake structure B40 extends into the cascade culture device B3, and the other end of the air intake structure B40 extends out of the cascade culture device B3 and is connected to the air intake branch A110, for delivering gas from the main gas supply pipe A100 into the cascade culture device B3.

[0104] In this embodiment, the main gas supply pipe A100 is connected to the gas supply equipment to provide stable pressure for cultivation air. Several cascade cultivation devices B3 can be connected in parallel to the main gas supply pipe A100 via the air intake branch A110. The cultivation air carried in the main gas supply pipe A100 is delivered to each cascade cultivation device B3 through the parallel multiple air intake branches A110. The cultivation air is then dispersed through the air intake structure B40 and forms a large number of fine bubbles in the culture solution, increasing the cultivation air content in the culture solution, thereby better promoting the growth and proliferation of plant tissues or cells.

[0105] Specifically, such as Figure 8 As shown, the gas supply main A100 and steam supply main B100 extend from the outer upright 3 on the right side of the main support frame X1011 to the second outer upright 31 on the left side of the second support frame X1012. The gas supply main A100 and steam supply main B100 are arranged in parallel from top to bottom on the back of the main support frame X1011. The cascade culture device B3 includes a seed tank 100 and a culture tank 200. Four air inlet structures B40 are evenly provided on the bottom side wall of the culture tank 200, and two air inlet structures B40 are evenly provided on the bottom side wall of the seed tank 100. The branch pipe B2 connecting the seed tank 100 with the gas supply main A100 and the steam supply main B100 is located in the biological culture system X1012. The arrangement of the main support frame X1011 of the 100 is basically the same as the arrangement of the branch pipes B2 between the culture tank 200 and the gas supply main A100 and the steam supply main B100 in the main support frame X1011 of the biological culture system X100. Taking the culture tank 200 as an example, the culture tank 200 is mapped on the back of the main support frame X1011 and close to its right side. The gas supply main A100 extends an air intake branch A110 near the right end of the main support frame X1011 and perpendicular to the back of the main support frame X1011 to the front. The air intake branch A110 extends to a preset distance and then bends several times to connect with the air intake structure B40 of the seed tank 100 and / or the culture tank 200.

[0106] like Figure 8-9As shown, taking the air inlet branch connecting the culture tank 200 as an example, the air inlet branch A110 includes a first air inlet branch A1101, a second air inlet branch A1102, a third air inlet branch A1103, a fourth air inlet branch A1104, a fifth air inlet branch A1105, an air inlet manifold A1106, and an air inlet branch pipe A1107. After extending a predetermined distance, the air intake branch A110 bends towards the ground to form a first air intake branch A1101. The first air intake branch A1101 extends below the steam supply main pipe B100. A second air intake branch A1102 is connected to the first air intake branch A1101 by bending and extending. The second air intake branch A1102 extends from the back of the vertical main support frame X1011 towards the front, and after extending a predetermined distance, bends and extends to form a third air intake branch A1103. The third air intake branch A1103 extends towards the ground to near the side wall of the culture tank 200. A fourth air intake branch A1104 extends from the bottom of the main support frame X1011, bending backwards. This fourth air intake branch A1104 extends vertically from the back of the main support frame X1011 towards the front. After extending a predetermined distance, it bends and extends to form a fifth air intake branch A1105. The fifth air intake branch A1105 bends and extends towards the left end of the main support frame X1011 and connects to a filter device A130. After extending between the culture tank 200 and the main support frame X1011, it bends and extends towards the ground, reaching a point below the bottom height of the culture tank 200. From there, it connects to the left and right sides of the main support frame X1011 via a three-way pipe. Two symmetrical air inlet pipes A1106 extend from the end of the culture tank 200. Taking one air inlet pipe A1106 as an example, the extension distance of the air inlet pipe A1106 is less than the maximum radius of the culture tank 200. After extending a predetermined distance, the air inlet pipe A1106 bends and extends towards the culture tank 200, passing through the main support frame X1011 and extending to the plane where the central axis of the culture tank 200 is located. This plane is parallel to the front of the main support frame X1011, so that the two air inlet pipes A1106 are symmetrically positioned below the culture tank 200 and close to the center of the bottom of the culture tank 200. At this point, the air intake manifold A1106 extends vertically upwards by a predetermined distance, and then extends two air intake branches A1107 to both sides through the air intake tee pipe B8. Finally, it bends upwards at a certain angle and connects to the two air intake structures B40 on the same side of the bottom of the culture tank 200. The other air intake manifold A1106 is symmetrically distributed therewith. The two air intake branches A1107 on the symmetrical side are connected to the two air intake structures B40 on the same side. The angle between the upward-bending section of the air intake branch pipe A1107 and the section extending through the air intake tee pipe B8 is less than 90°.In the direction of air intake, the intake branch A110 is divided into two branch pipes, and the two branch pipes are connected to an intake tee pipe B8. The intake tee pipe B8 then divides the incoming culture air into two more branch pipes, for a total of 4 branch pipes, which are connected to the 4 air intake structures B40 at the bottom of the culture tank 200. This ensures that the air is evenly supplied to the culture tank 200, which greatly improves the uniformity of the culture air entering the culture medium. Moreover, the pipes are hidden under the tank body, making full use of the space without affecting the arrangement of other pipes.

[0107] like Figure 8 As shown, taking the culture tank 200 as an example, at the connection between the second air inlet branch A1102 and the third air inlet branch A1103, there is a spare branch A160 connected in parallel with the third air inlet branch A1103. The spare branch A160 extends vertically upwards by a predetermined distance, then extends by a predetermined distance in a direction parallel to the fourth air inlet branch A1104, and finally extends vertically and connects with the fourth air inlet branch A1104. A manual valve B5 and a pneumatic valve B4 are installed on the spare branch A160. A pneumatic valve B4 is connected to the third air intake branch A1103, which adds a backup air intake line to the air supply system. This ensures that when the pneumatic valve B4 of the third air intake branch A1103 is damaged or under maintenance, the backup branch A160 can be used to maintain and / or regulate the culture air content of the culture medium in the culture tank 200, ensuring that the growth conditions of plant tissues or cells are stable and controllable, so as to maintain the continuous operation of the culture tank 200 and improve the reliability of the culture tank 200.

[0108] like Figure 7 As shown, in order to prevent the culture medium in the seed tank 100 and / or culture tank 200 from flowing into the air inlet branch A110, a check valve A120 is also provided in the air supply system. The check valve A120 is connected in series between the air inlet structure B40 and the air inlet branch A110.

[0109] In this embodiment, by adding a check valve A120 to the air intake section B41 of the air intake structure B40, the air intake branch A110 connected to the seed tank 100 and / or culture tank 200 can only deliver culture air to the inside of the seed tank 100 and / or culture tank 200 in one direction. This prevents the plant tissue and culture medium inside the seed tank 100 and / or culture tank 200 from flowing back into the pipeline, thereby improving the safety and reliability of the seed tank 100 and / or culture tank 200.

[0110] Because air contains a large number of microorganisms, in order to prevent microorganisms from multiplying in the culture medium, consuming the nutrients in the culture medium, or even causing plant tissues or cells to fail to grow and be cultivated normally, a filter device A130 is also installed in the air supply system.

[0111] Specifically, an air filter A130 is connected in series in the air intake branch A110. In this way, microorganisms are filtered out when the culture air passes through the air filter A130, thereby purifying the culture air entering the seed tank 100 and / or culture tank 200.

[0112] The filter elements of existing air filters A130 are typically made of materials such as polytetrafluoroethylene (PTFE). However, PTFE filter elements are easily punctured by microorganisms after a period of use, causing them to lose their ability to filter microorganisms, resulting in a short lifespan. To extend the lifespan of the filter elements and increase the maintenance cycle of air filters A130, the filter elements are made of silicate material.

[0113] In this embodiment, by improving the filter element material, the filter element is made of silicate material. The high hardness and high strength of silicate are used to improve the filter element's resistance to puncture, greatly extending the service life of the filter element and increasing the maintenance cycle of the filtration device A130. This allows the seed tank 100 and / or culture tank 200 to operate for a longer period of time, reducing the maintenance frequency.

[0114] To maintain a stable gas pressure within the seed tank 100 and / or culture tank 200, the gas supply system also includes an exhaust pipe A170. One end of the exhaust pipe A170 is connected to the top of the seed tank 100 and / or the culture tank 200, and the other end of the exhaust pipe A170 is connected to the outside, allowing the waste gas from the seed tank 100 and / or culture tank 200 to be directly discharged into the atmosphere.

[0115] In another embodiment, a gas filter A180 is also connected in series in the exhaust pipe A170 to trap the moisture and nutrients contained in the gas in the seed tank 100 and / or culture tank 200.

[0116] To ensure that the gas filter A180 remains at a low temperature for an extended period to improve the filtering effect on moisture and nutrients in the exhaust gas, the gas filter A180 is equipped with a cooling structure that covers the filter element, used to cool the gas filter A180.

[0117] Specifically, the cooling structure is equipped with cooling pipes for introducing and discharging liquids. These cooling pipes can be circulated with either low-temperature cooling water or low-temperature chilled water. The difference between chilled water and cooling water is that chilled water is 10-20 degrees Celsius cooler than cooling water.

[0118] In this embodiment, an exhaust pipe A170 is provided at the top of the seed tank 100 and / or culture tank 200. The exhaust pipe A170 and the air intake structure B40 control the gas entering and exiting the seed tank 100 and / or culture tank 200. This allows for stable gas pressure control within the seed tank 100 and / or culture tank 200 using the gas supply system. Furthermore, the intake and exhaust rates can be adjusted according to the optimal growth pressure for plant tissues or cells, ensuring that adventitious roots receive sufficient air. Simultaneously, a gas filter A180 is connected in series in the exhaust pipe A170 to prevent external microorganisms from entering the seed tank 100 and / or culture tank 200 through the exhaust pipe A170, and also to prevent moisture and nutrients from diffusing into the outside environment.

[0119] like Figure 8 As shown, taking the steam supply branch pipe B10 connected to the culture tank 200 as an example, the steam supply branch pipe B10 includes a filter-disinfection branch A140 and a tank-disinfection branch A150. One end of the filter-disinfection branch A140 and the tank-disinfection branch A150 is connected to the main steam supply pipe B100. The filter-disinfection branch A140 extends from the back of the main support frame X1011 to the front of the left side near the air inlet branch A110, perpendicular to the back of the main support frame X1011, to a position corresponding to the fifth air inlet branch A1105 in the same vertical plane, and then bends vertically to connect with the fifth air inlet branch A1105. The connection point is located between the air filter A130 and the fourth air inlet branch A1104. The filter-disinfection branch A140 is used to introduce steam to the air filter A130. High-temperature sterilization prevents microorganisms from entering the culture tank 200 through the air filter A130, thus affecting the operational stability of the biological culture system X100. The tank sterilization branch A150 is located to the left of the filter sterilization branch A140, extending perpendicularly from the back of the main support frame X1011 to its front, corresponding to the position of the fifth air inlet branch A1105 in the vertical plane. It then bends and extends perpendicularly to the ground, connecting with the fifth air inlet branch A1105. This branch is used to introduce high-temperature steam into the culture tank 200 to sterilize the culture medium before culturing plant tissues or cells. Furthermore, the steam supply main pipe B100 and the air supply main pipe A100 share a single pipe connected to the culture tank 200. This reduces the number of connection ports on the culture tank 200, simplifying the fabrication of the cascade culture device B3 and improving its sealing performance.

[0120] like Figure 8 As shown, the steam supply main pipe B100 is provided with a filtration branch A140 connected to the air intake branch A110. The connection end of the filtration branch A140 and the air intake branch A110 is located before the connection end of the inlet of the filter device A130 and the air intake branch A110.

[0121] In this embodiment, the air filter A130 and the steam supply main pipe B100 are connected by a filtration and disinfection branch A140. This allows the air filter A130 to be disinfected by high-temperature steam before the air supply system is turned on. This ensures that microorganisms on the air filter A130 are cleaned and that it is in good working condition, thereby improving the filtration effect of the air filter A130 and ensuring the cleanliness of the air entering the culture tank 200. This is beneficial for maintaining a good culture environment in the culture tank 200.

[0122] To allow high-temperature steam to be directly introduced into the culture tank 200, a tank-dissipation branch A150 connected to the culture tank 200 is also provided.

[0123] Specifically, one end of the tank disinfection branch A150 is connected to the main steam supply pipe B100, and the other end of the tank disinfection branch A150 is connected to the outlet of the filter device A130 and the connection end of the air inlet branch A110, so that the main steam supply pipe B100 and the main air supply pipe A100 share a single pipeline connected to the culture tank 200. This reduces the number of connection ports on the culture tank 200, which both reduces the difficulty of manufacturing the cascade culture device B3 and improves the sealing performance of the cascade culture device B3.

[0124] like Figure 5-7 As shown in the embodiment of the present invention, a piping structure of a cascade culture device B3 is described. Specifically, the piping structure includes a main pipeline B1 and branch pipelines B2. The main pipeline B1 and the cascade culture device B3 are separated.

[0125] Several main pipelines B1 extend linearly and parallel to each other along the main support frame X1011 and the second support frame X1012. The cascade culture device B3 is connected to branch pipelines B2 for exchanging and circulating substances with the main pipelines B1. One end of each branch pipeline B2 is connected to the main pipeline B1, passes through the main support frame X1011 and / or the second support frame X1012, and connects to the cascade culture device B3, thus linking the main pipelines B1 and the cascade culture device B3.

[0126] In this embodiment, the pipeline structure connected to the cascade culture device B3 is optimized and arranged according to the interception size. The main pipeline B1 has a large interception size for centralized material supply, while the branch pipeline B2 has a small interception size for introducing the material from the main pipeline B1 into the cascade culture device B3. The different main pipelines B1 are arranged in parallel to each other, which not only allows multiple branch pipelines B2 to be connected in the extension direction of each main pipeline B1, but also facilitates quick identification of each main pipeline B1 during maintenance, thereby improving the regularity of the pipeline structure and reducing the ineffective space occupation of the pipeline.

[0127] like Figure 2-7As shown in the embodiment of the present invention, a pipeline structure of a cascade culture device B3 is described. The device has several main pipelines B1 arranged in parallel with each other, and each main pipeline B1 carries a different medium.

[0128] Specifically, the main pipeline B1 includes a chilled water supply main pipeline C200 and a chilled water recovery main pipeline C100. Correspondingly, the branch pipeline B2 includes a chilled water supply branch pipeline C20 and a chilled water recovery branch pipeline C10.

[0129] One end of the chilled water supply branch pipe C20 is connected to the cascade culture device B3, and the other end is connected to the chilled water supply main pipe C200, for supplying chilled water to the cascade culture device B3; one end of the chilled water recovery branch pipe C10 is connected to the cascade culture device B3, and the other end is connected to the chilled water recovery main pipe C100, for draining the chilled water from the cascade culture device B3.

[0130] The main pipeline B1 includes a hot water supply main pipeline E200 and a hot water recovery main pipeline E100. Correspondingly, the branch pipeline B2 includes a hot water supply branch pipeline E20 and a hot water recovery branch pipeline E10.

[0131] One end of the hot water supply branch pipe E20 is connected to the cascade culture device B3, and the other end is connected to the hot water supply main pipe E200, for inputting hot water into the cascade culture device B3; one end of the hot water recovery branch pipe E10 is connected to the cascade culture device B3, and the other end is connected to the hot water recovery main pipe E100, for exporting the hot water in the cascade culture device B3.

[0132] The main pipeline B1 includes a cooling water supply main pipeline D200 and a cooling water recovery main pipeline D100. Correspondingly, the branch pipeline B2 includes a cooling water supply branch pipeline D20 and a cooling water recovery branch pipeline D10.

[0133] One end of the cooling water supply branch pipe D20 is connected to the cascade culture device B3, and the other end is connected to the cooling water supply main pipe D200, for supplying cooling water to the cascade culture device B3; one end of the cooling water recovery branch pipe D10 is connected to the cascade culture device B3, and the other end is connected to the cooling water recovery main pipe D100, for draining the cooling water from the cascade culture device B3.

[0134] In this embodiment, main pipelines B1 are provided for supplying and recovering media at different temperatures, and are connected to branch pipelines B2 corresponding to the main pipelines B1 to form a complete circulation loop. This allows the media in the main pipelines B1 to exchange heat with the cascaded culture device B3, thereby providing stable growth conditions for the proliferation of adventitious roots and other plant tissues. The parallel arrangement of the different main pipelines B1 makes the pipeline structure neat and easy to maintain.

[0135] In order to independently control the material circulation of the seed tank 100 and / or culture tank 200 in the cascade culture device B3, a pneumatic valve B4 for controlling the on / off of the pipeline is provided on the hot water supply branch pipe E20, the chilled water supply branch pipe C20 and the cooling water supply branch pipe D20.

[0136] In this embodiment, by connecting a pneumatic valve B4 in series on the pipeline, the pipeline connecting the cascade culture device B3 can achieve automated control of the on / off state, making the on / off control of the pipeline more convenient and rapid.

[0137] To improve the reliability of pipeline control and ensure that the pipeline can still switch between on and off states even when the pneumatic valve B4 fails or is under maintenance, thus maintaining the continuous operation of the cascade culture device B3, manual valves B5 for controlling the on / off state of the pipeline are also installed on the hot water supply branch E20, the chilled water supply branch C20, and the cooling water supply branch D20.

[0138] Specifically, the manual valve B5 and the pneumatic valve B4 are connected in series.

[0139] In this embodiment, by connecting a manual valve B5 in series on the pipeline, the pipeline connecting the cascade culture device B3 can still change its on / off state when the pneumatic valve B4 fails or is under maintenance, so as to maintain the continuous operation of the cascade culture device B3 and improve the reliability of the pipeline structure.

[0140] To reduce the number of connection ports on the cascade culture device B3, the hot water supply branch pipe E20, the chilled water supply branch pipe C20, and the cooling water supply branch pipe D20 are all connected to an input pipe B6 on the cascade culture device B3; the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10, and the cooling water recovery branch pipe D10 are all connected to an output pipe B7 on the cascade culture device B3.

[0141] In this embodiment, by setting an input pipe B6 and an output pipe B7 on the cascade culture device B3, and connecting the hot water supply branch pipe E20, the chilled water supply branch pipe C20, and the cooling water supply branch pipe D20 connected to the input pipe B6 in parallel, and connecting the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10, and the cooling water recovery branch pipe D10 connected to the output pipe B7 in parallel, the number of connection ports of the cascade culture device B3 is reduced. This reduces the difficulty of manufacturing the cascade culture device B3 and improves its sealing performance.

[0142] To improve the safety and reliability of the pipeline structure, a loop is provided in the main pipeline B1 to directly discharge the high-heat medium, facilitating the maintenance of the circulation loop. The main pipeline B1 also includes a heat exhaust pipe C400 equipped with a pressure-reducing filter 410. A discharge branch 420 connecting the input pipe B6 and the branch pipeline B2 to the pressure-reducing filter 410 is provided, allowing the medium supplied in the main pipeline B1 to flow directly into the heat exhaust pipe C400 without passing through the cascade culture device B3.

[0143] In another embodiment, in order to ensure that the branch pipe B2 can accurately form a circulation loop after sharing an input pipe B6, a pneumatic valve B4 and / or a manual valve B5 for controlling the opening and closing of the pipes are provided on the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10 and the cooling water recovery branch pipe D10.

[0144] The pneumatic valves B4 and / or manual valves B5 on the hot water recovery branch pipe E10, the chilled water recovery branch pipe C10, and the cooling water recovery branch pipe D10 are used to correspond and match with the pneumatic valves B4 and / or manual valves B5 on the hot water supply branch pipe E20, the chilled water supply branch pipe C20, and the cooling water supply branch pipe D20, thereby forming a complete circulation loop.

[0145] like Figure 5-7 As shown in the embodiment of the present invention, the biological culture system X100 includes a cascade culture device B3, which is equipped with a temperature control system controlled by a sub-control system. The temperature control system includes a supply unit A1, a temperature control layer A31 covering the outer surface of the cascade culture device B3, and a conveying unit A2 connecting the supply unit A1 and the temperature control layer A31. The supply unit A1 is capable of carrying multiple media at different temperatures and introducing them into and / or exporting them from the temperature control layer A31 via the conveying unit A2. The supply unit A1 includes several parallel main pipelines B1, each of which is used to introduce or export different media into or from the cascade culture device B3 or the temperature control layer A31.

[0146] Specifically, the temperature control system also includes at least a temperature-controlled water tank A3 connected to the supply unit A1. The inlet of the temperature-controlled water tank A3 is connected to the liquid recovery main pipe X12, and the outlet of the temperature-controlled water tank A3 is connected to the liquid supply main pipe X11, allowing the medium to circulate between the bioreactor X12 and the temperature-controlled water tank A3. The temperature-controlled water tank A3 is connected in parallel to the seed tank 100 and / or the culture tank 200 on the supply unit A1. The supply unit A1 can carry multiple media at different temperatures and introduce them into and out of the temperature-controlled water tank A3 via the conveying unit A2. The media in the temperature-controlled water tank A3 circulates to the temperature control layer A31, exchanges heat with the seed tank 100 and / or the culture tank 200, and then flows back into the temperature-controlled water tank A3.

[0147] In this embodiment, both the temperature-regulating water tank A3 and the temperature-controlling layer A31 are connected to the supply unit A1. The temperature-regulating water tank A3 and the temperature-controlling layer A31 can simultaneously exchange heat media with the supply unit A1. Furthermore, the temperature-regulating water tank A3 and the temperature-controlling layer A31 can be connected in series by the supply unit A1 to form a temperature-regulating circuit, allowing direct circulation between the temperature-regulating water tank A3 and the temperature-controlling layer A31. This ensures that the temperature-regulating circuit can independently regulate the temperature of the culture tank 200 when the supply source of the temperature-regulating system is damaged or under maintenance, thereby improving the fault tolerance of the temperature-regulating system.

[0148] Specifically, the temperature-regulating water tank A3 is provided with a water-containing cavity A301, which is used to hold and heat the medium that can be input into the temperature-controlling layer A31, thereby forming a circulation loop between the temperature-regulating water tank A3 and the temperature-controlling layer A31 that can supply heat to the temperature-controlling layer A31.

[0149] The water-containing chamber A301 and the temperature-controlled layer A31 are connected to the supply unit A1 via the conveying unit A2 to form a circulation loop. The conveying unit A2 includes an inlet pipe A21 and an outlet pipe A22 that connect the supply unit A1 and the water-containing chamber A301, and the temperature of the seed tank 100 and / or the culture tank 200 can be maintained by the temperature-controlled water tank A3.

[0150] In this embodiment, the supply unit A1 can simultaneously supply the medium to the temperature-regulating water tank A3 and the temperature control layer A31, and can also supply the medium from the temperature-regulating water tank A3 to the temperature control layer A31, thereby making the temperature-regulating water tank A3 a backup heat source for the temperature regulation system, which can ensure that the seed tank 100 and / or the culture tank 200 can maintain and regulate the temperature of the culture medium in the seed tank 100 and / or the culture tank 200 normally when the supply unit A1 is damaged or under maintenance.

[0151] like Figure 6-7 As shown in the embodiments of the present invention, a temperature control system for a seed tank 100 and / or a culture tank 200 is described. In this temperature control system, a temperature-controlled water tank A3 is provided with a heat exchange structure A23. In order to save resources, the heat exchange structure A23 is connected to a supply unit A1, and the medium in the water chamber A301 can be heated or cooled by the supply unit A1.

[0152] Specifically, the temperature-regulating water tank A3 has a heat exchange structure A23 disposed against the water-containing cavity A301. The conveying unit A2 includes an inlet pipe A24 and an outlet pipe A25 with multiple branches connecting the supply unit A1 and the heat exchange structure A23, for introducing and discharging media of different temperatures into and out of the heat exchange structure A23 respectively.

[0153] The temperature-regulating water tank A3 is also equipped with a central control unit that is electrically connected to the pneumatic valves B4 connected in series in each branch of the liquid inlet pipe A24. The central control unit is used to control the proportion of medium delivered from the liquid inlet pipe A24 to the heat exchange structure A23 of the temperature-regulating water tank A3.

[0154] Specifically, the central control unit is electrically connected to the pneumatic valves B4 connected in series in each branch of the inlet pipe A24, enabling it to adjust the flow rate ratio of the medium supplied to the temperature-controlled water tank A3 and the heat exchange structure A23. When the medium enters the temperature-controlled water tank A3 or the heat exchange structure A23, it can exchange heat with another medium in the water-containing chamber A301, thereby ensuring that the medium supplied to the temperature-controlled layer A31 meets the needs of the seed tank 100 and / or the culture tank 200. Furthermore, when the supply unit A1 cannot directly supply the medium to the temperature-controlled layer A31, hot water can be supplied from the temperature-controlled water tank A3 to maintain the operation of the seed tank 100 and / or the culture tank 200.

[0155] In another embodiment, the temperature-regulating water tank A3 can be equipped with an electric heating structure to directly heat the medium in the water-containing chamber A301. Therefore, when hot water cannot be directly supplied to the temperature-controlled layer A31 from the hot water supply main pipe E200, the hot water supply main pipe E200 and the hot water recovery main pipe E100 can be directly connected to the temperature-controlled layer A31 and the temperature-regulating water tank A3. This forms a separate circulation loop between the temperature-regulating water tank A3 and the temperature-controlled layer A31, enabling the direct supply of hot water from the temperature-regulating water tank A3 to the temperature-controlled layer A31, ensuring the temperature requirements of the seed tank 100 and / or the culture tank 200.

[0156] Specifically, during normal operation of the supply unit A1, the temperature control layer A31 can be supplied with hot water directly from the hot water supply main pipe E200, or simultaneously with hot water from the temperature-regulating water tank A3. In this case, the temperature of the hot water in the water-containing chamber A301 can be further increased before being supplied to the hot water supply main pipe E200, which can provide temperature compensation for the hot water in the hot water supply main pipe E200, thereby allowing for more precise adjustment and control of the temperature of the seed tank 100 and / or the culture tank 200.

[0157] In this embodiment, by setting a heat exchange structure A23 in the temperature-controlled water tank A3, the medium in the temperature-controlled water tank A3 is kept at the set temperature during the operation of the supply unit A1. This ensures that the temperature-controlled water tank A3 can immediately supply the required medium to the temperature control layer A31 when the supply unit A1 stops supplying or is under maintenance. This saves the time that the temperature-controlled water tank A3 needs to adjust the temperature of the medium inside it, and ensures that the backup heat exchange circuit of the temperature control system can quickly take over from the main heat exchange circuit to maintain the cultivation temperature of the seed tank 100 and / or the culture tank 200.

[0158] The supply unit A1 includes a hot water supply main pipe E200 and a hot water recovery main pipe E100 arranged in parallel. The conveying unit A2 includes a hot water supply branch pipe E20 and a hot water recovery branch pipe E10. One end of the hot water supply branch pipe E20 is connected to the hot water supply main pipe E200 and the other end is connected to the temperature control layer A31. One end of the hot water recovery branch pipe E10 is connected to the temperature control layer A31 and the other end is connected to the hot water recovery main pipe E100.

[0159] The inlet pipe A21 is connected to the hot water supply main pipe E200, and the outlet pipe A22 is connected to the hot water recovery main pipe E100, connecting the water container A301 and the temperature control layer A31 to form a circulation loop.

[0160] A water pump A211 is connected in series in the water inlet pipe A21 to transport water from the water storage chamber A301 to the temperature control layer A31 through the hot water supply main pipe E200.

[0161] In this embodiment, the supply unit A1 is equipped with a hot water supply main pipe E200 and a hot water recovery main pipe E100 for supplying and recovering hot water. These are connected to form a complete circulation loop via corresponding branch pipes B2, allowing media of different temperatures in the supply unit A1 and the temperature-controlled water tank A3 to exchange heat with the seed tank 100 and / or the culture tank 200, thereby providing stable growth conditions for the proliferation of adventitious roots and other plant tissues. A water pump A211 is connected in series with the inlet pipe A21 of the temperature-controlled water tank A3 to provide driving force for the circulation loop formed by the temperature-controlled water tank A3 and the temperature-controlled layer A31, realizing the circulation of the media between the temperature-controlled water tank A3 and the temperature-controlled layer A31.

[0162] To reduce the resistance of the medium transported between the supply unit A1 and the temperature-regulating water tank A3, and to prevent the water pump A211 from blocking the flow of the medium into the temperature-regulating water tank A3, a pump branch pipe A212 is installed on the inlet pipe A21 in parallel with the water pump A211. The two ends of the pump branch pipe A212 are respectively connected to the inlet end of the water pump A211 and the outlet end of the inlet pipe A21, thus allowing the medium flowing from the supply unit A1 into the temperature-regulating water tank A3 to flow through the pump branch pipe A212 and bypass the water pump A211.

[0163] In this embodiment, by connecting a pump branch pipe A212 in parallel to the water inlet pipe A21 equipped with a water pump A211, the resistance of the supply unit A1 in conveying the medium to the temperature-regulating water tank A3 is reduced.

[0164] To prevent the temperature of the medium in the temperature-regulating water tank A3 from becoming too high, the supply unit A1 includes a chilled water supply main pipe C200 and a cold air vent pipe C300 connected in parallel, which are used to transport cooling water to the heat exchange structure A23 and transfer heat with the medium in the water chamber A301 to reduce the temperature of the medium in the temperature-regulating water tank A3.

[0165] Specifically, the inlet pipe A24 includes a chilled water inlet pipe A241, and the outlet pipe A25 includes a chilled water outlet pipe A251. One end of the chilled water inlet pipe A241 is connected to the chilled water supply main pipe C200, and the other end is connected to the heat exchange structure A23. One end of the chilled water outlet pipe A251 is connected to the heat exchange structure A23, and the other end is connected to the radiator pipe C300.

[0166] In this embodiment, the supply unit A1 is equipped with a chilled water supply main pipe C200 and a cold air radiator pipe C300, which are connected to the heat exchange structure A23. This allows the temperature control system to quickly adjust the heat exchange structure A23 to exchange heat with the temperature control water tank A3, thereby preventing the medium temperature in the temperature control water tank A3 from becoming too high and ensuring that the medium in the temperature control water tank A3 is always kept at the set temperature.

[0167] In order to increase the temperature of the medium in the temperature-regulating water tank A3, the supply unit A1 includes a steam supply main pipe B100 and a heat exhaust pipe C400 arranged in parallel, which are used to transport steam to the heat exchange structure A23 and transfer heat to the medium in the water chamber A301 to increase the temperature of the medium in the temperature-regulating water tank A3.

[0168] Specifically, the liquid inlet pipe A24 includes a steam liquid inlet pipe A242, and the liquid outlet pipe A25 includes a steam liquid outlet pipe A252. One end of the steam liquid inlet pipe A242 is connected to the steam supply main pipe B100, and the other end is connected to the heat exchange structure A23. One end of the steam liquid outlet pipe A252 is connected to the heat exchange structure A23, and the other end is connected to the heat exhaust pipe C400.

[0169] In this embodiment, the supply unit A1 is equipped with a steam supply main pipe B100 and a heat exhaust pipe C400, which are connected to the heat exchange structure A23. This allows the temperature control system to quickly adjust the heat exchange structure A23 to exchange heat with the temperature control water tank A3, thereby preventing the medium temperature in the temperature control water tank A3 from being too low and ensuring that the medium in the temperature control water tank A3 is always kept at the set temperature.

[0170] To ensure the reliability and safety of the control steam inlet pipe A242, two parallel branches are provided on the steam inlet pipe A242: one is connected in series with a manual valve B5 and a pneumatic valve B4, and the other is connected in series with only a manual valve B5, which is used to manually control the opening and closing of the steam inlet pipe A242 when the automatic control fails.

[0171] A steam outlet pipe A252 connects the temperature-regulating water tank A3 to the heat dissipation pipe C400. At the end of the steam outlet pipe A252 connected to the temperature-regulating water tank A3, it splits into two pipes: one with a one-way valve and a pneumatic valve B4 connected in series, and the other with only the pneumatic valve B4 connected in series.

[0172] In this embodiment, by setting two parallel pipelines for controlling the on / off state on the steam inlet pipe A242 and the steam outlet pipe A252 respectively, the steam circulation loop of the temperature regulating water tank A3 can be automatically controlled by pneumatics, and the on / off state of the steam circulation loop can be manually switched when the automatic control fails or maintenance is required. This avoids the situation where the supply unit A1 cannot be used to heat the temperature regulating water tank A3, and improves the stability and reliability of the temperature regulating system.

[0173] During the transmission of the medium, the temperature of the medium may change slightly due to heat transfer between the medium and the pipes and the outside environment. If the medium with the changed temperature is directly introduced into the temperature control layer A31, the temperature of the culture medium in the seed tank 100 and / or culture tank 200 will be inconsistent with the preset temperature, which will have a negative impact on the culture of plant tissues.

[0174] In this embodiment, the medium passing through the longitudinal pipeline C1 and the main pipeline B1 can first be transported to the temperature-controlled water tank A3 for heating or cooling treatment so that the temperature of the medium meets the cultivation requirements. Then, the medium is transported to the temperature control layer A31 to ensure that the temperature of the culture medium in the seed tank 100 and / or the culture tank 200 is within the preset temperature range.

[0175] As one implementation method, such as Figure 7 As shown, first open the valve on the pump branch pipe A212, which is connected to the inlet pipe A21. The medium flows from the hot water supply main pipe E200 through the inlet pipe A21 and the pump branch pipe A212 into the water chamber A301 of the temperature-regulating water tank A3. Then open the valve on the outlet pipe A22 to connect the water chamber A301 of the temperature-regulating water tank A3 with the hot water recovery main pipe E100. Finally, the water chamber A301 of the temperature-regulating water tank A3 is filled with the medium that needs to be regulated. Then close the valve on the pump branch pipe A212. Open the valves on the branch pipe B2 connecting the hot water supply main pipe E200 and the temperature control layer A31, and the valves on the branch pipe B2 connecting the hot water recovery main pipe E100 and the temperature control layer A31, so that the water chamber A301 of the temperature-regulating water tank A3 is connected to the temperature control layer A31 to form a circulation loop. When the medium introduced into the temperature control layer A31 causes the culture medium temperature to be too low, an appropriate amount of steam is introduced into the temperature regulating water tank A3 to raise the medium temperature. The medium is then pumped to the temperature control layer A31 via water pump A211 to raise the culture medium temperature to a suitable culture temperature and maintain the culture medium temperature within the culture temperature range. Conversely, when the medium introduced into the temperature control layer A31 causes the culture medium temperature to be too high, an appropriate amount of chilled water is introduced into the temperature regulating water tank A3 to lower the medium temperature. The medium is then pumped to the temperature control layer A31 via water pump A211 to lower the culture medium temperature to a suitable culture temperature and maintain the culture medium temperature within the culture temperature range.

[0176] When a biological culture system X100 malfunctions, its liquid supply main pipe X11 needs to be connected to the liquid supply main pipe X11 of another normally functioning biological culture system X100. Specifically, the temperature-regulating water tank A3 of the other normally functioning biological culture system X100 needs to regulate the temperature of the medium supplied to the malfunctioning biological culture system X100, ensuring that the medium, after entering the temperature control layer A31, maintains the culture medium in the bioreactor X102 at an appropriate culture temperature, so that the plant tissues in the culture medium can proliferate and grow normally.

[0177] like Figure 1-4 As shown, in this embodiment, the total support device X101 includes a total support frame X1011 and a second support frame X1012, as detailed below:

[0178] The main support frame X1011 includes two vertically parallel outer uprights 3. Each outer upright 3 has two parallel outer horizontal bars 1 connected to its two ends. The two ends of each horizontal bar 1 are perpendicularly connected to the two ends of the outer uprights 3 in the vertical direction. The outer uprights 3 and horizontal bars 1 form the back of the main support frame X1011. Opposite to the back is a parallel front. The front includes two parallel inner uprights 4. Each inner upright 4 has two parallel inner horizontal bars 2 connected to its two ends in the vertical direction. The two ends of the inner upright 4 are vertically connected. The inner upright 4 is parallel to the outer upright 3. The inner horizontal bar 2 is parallel to the outer horizontal bar 1. The ends of adjacent inner upright 4 and outer upright 3 are connected to form two inner longitudinal bars 5 and two outer longitudinal bars 6. The inner longitudinal bars 5 are perpendicular to the outer upright 3 in the vertical direction and perpendicular to the outer horizontal bar 1 in the horizontal direction. The outer longitudinal bars 6 are perpendicular to the outer upright 3 in the vertical direction and perpendicular to the outer horizontal bar 1 in the horizontal direction. The outer horizontal bar 1, inner horizontal bar 2, outer upright 3, inner upright 4, inner longitudinal bar 5 and outer longitudinal bar 6 are connected to form a hollow overall support frame X1011.

[0179] Specifically, the plane formed by the outer upright 3 and the outer horizontal bar 1 is the back of the main support frame X1011, the plane formed by the inner upright 4 and the inner horizontal bar 2 is the front of the main support frame X1011, the plane formed by the inner upright 4, the outer upright 3 and the inner vertical bar 5 is the left end of the main support frame X1011, and the plane formed by the inner upright 4, the outer upright 3 and the outer vertical bar 6 is the right end of the main support frame X1011.

[0180] Between the outer horizontal bar 1 and the inner horizontal bar 2 at the height of the main support frame X1011, several upper support longitudinal bars 7 are evenly distributed and connected to them. The upper support longitudinal bars 7 are parallel to the inner longitudinal bars 5 and the outer longitudinal bars 6, which are in the same plane. Between the outer horizontal bar 1 and the inner horizontal bar 2 at the low height of the main support frame X1011, several lower support longitudinal bars 8 are evenly distributed and connected to them. The lower support longitudinal bars 8 are parallel to the inner longitudinal bars 5 and the outer longitudinal bars 6, which are in the same plane. Between the inner horizontal bars 2, several inner support uprights 9 are evenly distributed and connected to them. The inner support uprights 9 are parallel to the inner uprights 4. Between the outer horizontal bars 1, several outer support uprights 10 are evenly distributed and connected to them. The outer support uprights 10 are parallel to the outer uprights 3.

[0181] In this embodiment of the invention, there are two upper support longitudinal rods 7, three lower support longitudinal rods 8, one inner support upright rod 9, and two outer support upright rods 10.

[0182] The main support frame X1011 has a second support frame X1012 at one end. The second support frame X1012 includes two parallel outer horizontal bars 11 and inner horizontal bars 21 extending along the direction of the outer horizontal bar 1 and the inner horizontal bar 2. The two ends of the second outer horizontal bar 11 are connected to parallel outer vertical bars 31. The second outer vertical bars 31 and the second outer horizontal bar 11 are perpendicular to each other in the vertical direction. The two ends of the second inner horizontal bar 21 are connected to parallel inner vertical bars 41. The two ends of the second outer horizontal bar 11 and the second inner horizontal bar 21 on the same plane are respectively connected to a second inner longitudinal bar 51 and a second outer longitudinal bar 61. The second inner longitudinal bar 51 and the second inner horizontal bar 21 are perpendicular to each other in the horizontal direction. The second outer longitudinal bar 61 and the second inner horizontal bar 21 are perpendicular to each other in the horizontal direction.

[0183] The second support frame X1012 is provided with two parallel second outer support longitudinal rods 71. The two ends of the second outer support longitudinal rods 71 ​​are perpendicularly connected to the second outer cross rod 11 and the second inner cross rod 21 in the same plane, respectively. The second outer support longitudinal rods 71 ​​and the second inner longitudinal rods 51 are parallel to each other.

[0184] The second support frame X1012 also includes a second inner support column 91 and a second outer support column 101. The two ends of the second inner support column 91 are perpendicularly connected to the second inner horizontal bar 21 in the same plane; the two ends of the second outer support column 101 are perpendicularly connected to the second outer horizontal bar 11 in the same plane; the second inner support column 91 and the second inner column 41 are parallel to each other in the same vertical plane, and the second outer support column 101 and the second outer column 31 are parallel to each other in the same vertical plane. The second outer support longitudinal bar 71, the second inner support column 91, and the second outer support column 101 are all on the same vertical plane; a second inner support longitudinal bar 611 connects the second inner support column 91 and the second outer support column 101. The two ends of the second inner support longitudinal rod 611 are perpendicularly connected to the second inner support upright rod 91 and the second outer support upright rod 101, respectively. On the side of the second support frame X1012 near the end of the main pipeline B1, between the second outer upright rod 31 and the second inner upright rod 41, another second inner support longitudinal rod 611 is connected. The two ends of the second inner support longitudinal rod 611 are perpendicularly connected to the second outer upright rod 31 and the second inner upright rod 41 in the same vertical plane, respectively. A second outer support horizontal rod 211 is connected between the second inner upright rod 41 and the second inner support upright rod 91. The two ends of the second outer support horizontal rod 211 are perpendicularly connected to the second inner upright rod 41 and the second inner support upright rod 91 in the same vertical plane, respectively. The second outer support horizontal rod 211 and the second inner support longitudinal rod 611 are on the same horizontal plane.

[0185] In another embodiment, the second outer crossbar 11 and the second inner crossbar 21 may also be extensions of the outer crossbar 1 and the inner crossbar 2. The inner longitudinal bar 5 and the second inner longitudinal bar 51 at the connection between the main support frame X1011 and the second support frame X1012 are combined into the upper support longitudinal bar 7 and the lower support longitudinal bar 8. The outer upright bar 3 and the second outer upright bar 31 are combined into the outer support upright bar 10. The inner upright bar 4 and the second inner upright bar 41 are combined into the inner support upright bar 9. The second outer upright bar 31 at the non-connection point is the outer upright bar 3, the second inner upright bar 41 is the inner upright bar 4, and the second outer longitudinal bar 61 is the inner longitudinal bar 5.

[0186] The plane formed by the connection of the second outer support crossbar 211 and the second inner support longitudinal bar 611 divides the hollow space formed by the connection of the second outer crossbar 11, the second inner crossbar 21, the second outer vertical bar 31, the second inner vertical bar 41, the second outer longitudinal bar 61, the second outer support vertical bar 101, the second inner support vertical bar 91 and the second outer support longitudinal bar 71 into two storage spaces, the upper part being the first space and the lower part being the second space. The space is used to place the control devices required in the biological culture system X100 and / or the equipment for processing the medium required for biological culture.

[0187] In this embodiment of the invention, a control cabinet is placed in the first space, and a temperature-regulating water tank A3 is placed in the second space. In the first space, the distance between the second outer support crossbar 211 and the second inner crossbar 21 above it, which is on the same vertical plane, is greater than or equal to the height of the control cabinet. In the second space, the distance between the second outer support crossbar 211 and the second inner crossbar 21 below it, which is on the same horizontal plane, is greater than or equal to the height of the temperature-regulating water tank A3. The length of the second outer longitudinal bar 61 is greater than the width of the temperature-regulating water tank A3 and the control cabinet. The bottom of the control cabinet is fitted and erected in the first space with the second outer support crossbar 211 and the second inner support longitudinal bar 611. The bottom of the temperature-regulating water tank A3 is fitted and erected in the second space with the second outer longitudinal bar 61, the second inner crossbar 21, and the second outer support longitudinal bar 71.

[0188] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A modular bioprocessing plant comprising a plurality of independently arranged bioprocessing systems (X100) and a general control system, the bioprocessing systems (X100) comprising: At least one bioreactor (X102), a general support device (X101) arranged on one side of the bioreactor (X102), and a sub-control system arranged inside the general support device (X101), characterized in that, The back of the general support device (X101) is provided with a plurality of main pipes (B1) in the horizontal direction and a plurality of longitudinal pipes (C1) respectively connected with the main pipes (B1) in the vertical direction; The main pipes (B1) are connected with sub-pipes (B2) which extend through the general support device (X101) to the direction of the bioreactor (X102) and are directly or indirectly connected with the bioreactor (X102); The general support device comprises a general support frame and a second support frame arranged adjacently, and the general support frame and the second support frame are hollow support frames formed by a plurality of horizontal rods, vertical rods and vertical rods connected with each other; the main pipes, the sub-pipes and the longitudinal pipes are fixed on the general support device; At least one end of the main pipe (B1) is provided with a detachable sealing device; A general control system is in communication connection with each sub-control system; the modular biological culture workshop further comprises independent pipe connecting pieces which can connect the main pipe with a fault in the biological culture system with the normally working main pipe in the corresponding another biological culture system after the sealing device is detached, so as to ensure that the material can be transferred between different biological culture systems when the general control system and / or the sub-control system shows that the main pipe and / or the longitudinal pipe is faulty.

2. A modular bio-cultivation plant according to claim 1, characterized in that, A plurality of independently arranged biological culture systems (X100) are arranged at intervals, and the installation position of the main pipe (B1) of the biological culture system (X100) corresponds to the installation position of the main pipe (B1) of the adjacent another biological culture system (X100).

3. A modular bio-cultivation plant according to claim 2, characterized in that, The main pipes (B1) are uniformly distributed in the vertical direction on the general support device (X101), and the longitudinal pipes (C1) are concentratedly distributed in the horizontal direction on the general support device (X101), and the communication position of the longitudinal pipe (C1) and the main pipe (B1) is close to the middle part of the main pipe (B1).

4. A modular bio-cultivation plant according to claim 3, characterized in that, The connecting line of the end part of the main pipe (B1) is perpendicular to the horizontal plane, and the position of the end part of the main pipe (B1) does not exceed the edge of the general support device (X101).

5. A modular bio-cultivation plant according to any one of claims 1-4, characterized in that, The main pipe (B1) comprises: A gas supply main pipe (A100) for supplying culture gas to the inside of the bioreactor (X102); A steam supply main pipe (B100) for supplying steam to the inside of the bioreactor (X102) and the auxiliary device or the temperature control layer (A31) of the bioreactor (X102); A liquid supply main pipe (X11) for supplying liquid to the temperature control layer (A31) of the bioreactor (X102); A liquid recovery main pipe (X12) for recovering the liquid supplied to the temperature control layer (A31) of the bioreactor (X102); The sub-pipeline (B2) is provided with a plurality of sub-pipelines (B2), one end of each of the sub-pipelines (B2) is connected to one of the four main pipelines, i.e., the gas supply main pipeline (A100), the steam supply main pipeline (B100), the liquid supply main pipeline (X11) and the liquid recovery main pipeline (X12), and the other end of each of the sub-pipelines (B2) is connected to the bioreactor (X102) directly or through a pipeline, and the on-off of the sub-pipeline (B2) is controlled by the corresponding sub-pipeline valve to deliver the medium.

6. A modular bio-cultivation plant according to claim 5, characterized in that, The sub-control system is connected to the sub-pipeline valve, and the sub-control system can control the opening and closing of the sub-pipeline valve to control the delivery speed and delivery time of the culture gas, steam and liquid into the bioreactor (X102). The biological culture system (X100) is provided with a temperature regulating system, and the temperature regulating system at least includes a temperature regulating water tank (A3), the inlet of the temperature regulating water tank (A3) is connected to the liquid recovery main pipeline (X12), and the outlet of the temperature regulating water tank (A3) is connected to the liquid supply main pipeline (X11) to circulate the medium between the bioreactor (X102) and the temperature regulating water tank (A3).

7. A modular bio-cultivation plant according to claim 6, characterized in that, The sub-control system detects the amount and / or temperature of the medium delivered into the biological culture system (X100) and the culture parameter data of the bioreactor (X102) in real time, and when the detected data of the sub-control system is different from the preset value and the duration reaches the preset time, the sub-control system sends a warning information; the data of the sub-control system is transmitted to the total control system in real time.

8. A modular bio-cultivation plant according to claim 7, characterized in that, The total control system is connected to the pipeline connector (Y1); when the sub-control system sends a warning information, the total control system controls the pipeline connector (Y1) to connect the main pipeline (B1) in the biological culture system (X100) which fails to the main pipeline (B1) in the corresponding another biological culture system (X100) which works normally; when the main pipeline (B1) which fails works normally after being repaired, the detected data of the sub-control system is the same as the preset value, and the duration reaches the preset time, the total control system controls the pipeline connector (Y1) to disconnect the two main pipelines (B1).

9. The modular bio-cultivation plant according to claim 1, characterized in that, The total support frame (X1011) and the second support frame (X1012) are adjacent independent frame structures, or frame structures sharing horizontal rods, vertical rods and vertical rods.

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