A method for preparing large seaweed primary color specimens

Abstract

This invention discloses a method for preparing large-scale, original-color seaweed specimens, belonging to the field of seaweed preparation methods. The method includes the following steps: S1: Collecting algal bodies with reproductive organs and basal holdfasts; S2: Washing the algal bodies from S1 with pretreated water, selecting algal bodies with intact reproductive organs and basal holdfasts, and drying them; S3: Completely wrapping the algal bodies from S2 with plastic wrap and irradiating them with ultraviolet light; S4: Preparing a preservation solution; S5: Immersing the algal bodies from S3 in the preservation solution; removing them after 5 minutes; S6: Preparing the original-color specimen: removing the algal bodies from S5, placing them in a paper bag, and air-drying them in a dry, ventilated location. The dried specimen is then the original-color specimen. This method can produce original-color seaweed specimens.

Description

Technical Field

[0001] This invention pertains to methods for preparing seaweed specimens, specifically a method for preparing large, original-color seaweed specimens. Background Technology

[0002] For teaching and research purposes, seaweed specimens are usually made.

[0003] Authorization announcement number CN 101548670 B discloses a method for preparing seaweed specimens, which solves the problem of gel outflow affecting the weak adhesion of the specimens.

[0004] However, in practice, for seaweed specimens prepared using the drying method, bacteria on the seaweed surface can affect the original color of the seaweed specimen during the air-drying process. Bacteria active on the seaweed specimen can easily cause spot-like contamination on the surface, affecting the integrity of the original color range. Traditional sterilization methods mainly rely on sulfurous acid. The sterilization effect of sulfurous acid is affected by temperature and contact time. Soaking in sulfurous acid for too long can bleach the seaweed, affecting its original color; if the time is too short, the sterilization effect will be compromised. The same applies to ultraviolet (UV) lamps; UV lamps also affect the original color of the specimen and rapidly dehydrate it, affecting its shape. Therefore, it is necessary to consider both sterilization and preservation of the seaweed's original color and shape. Summary of the Invention

[0005] The present invention provides a method for preparing large-scale seaweed specimens in their original color, which can simultaneously sterilize the seaweed while maintaining its original color and shape.

[0006] To achieve the above objectives, the present invention provides a method for preparing a large primary color specimen of seaweed, comprising the following steps:

[0007] S1: Collect algae with reproductive organs and basal holdfasts;

[0008] S2: Use pretreated water to wash the algae in S1, select algae with intact reproductive organs and basal holdfasts, and dry them.

[0009] S3: Completely wrap the algae in S2 with plastic wrap and irradiate the wrapped algae with ultraviolet light.

[0010] S4: Prepare the preservation solution;

[0011] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0012] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens.

[0013] Further, in step S3, the irradiation time of the ultraviolet light is 8-10 minutes.

[0014] Further, in step S3, the wrapping thickness of the preservative film is 2 layers.

[0015] Further, in step S2, the pretreatment water is fresh water and seawater in a volume ratio of 1:1; and the washed algal body is dried with a water absorption paper.

[0016] Further, in step S4, the storage solution is uniformly mixed from sulfurous acid, glycerol and water in a volume ratio of 4:10:86.

[0017] Beneficial effects:

[0018] The method can kill bacteria by ultraviolet light, reduce the damage of bacteria to the original color of the specimen, and then reduce the water loss of the seaweed caused by ultraviolet irradiation by the preservative film, so as to maintain the original color and shape of the seaweed. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 is a comparison chart of the comparative example 1 and the comparative example 2 of the method;

[0020] Figure 2 is a flow chart of the method. DETAILED DESCRIPTION

[0021] To make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the present application will be described clearly and completely below with reference to the drawings. Obviously, the described embodiments are some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application. Embodiment 1:

[0022] A method for making a large seaweed specimen with original color, comprising the following steps:

[0023] S1: for the attached algae, the algal body with reproductive organs and base fixer is taken;

[0024] The attached algae, such as brown algae and red algae, are selected and cut in the intertidal zone and subtidal zone with a knife to obtain the algal body with reproductive organs and base fixer.

[0025] S2: the algal body in S1 is washed with pretreatment water, and the algal body with complete reproductive organs and base fixer is selected and dried;

[0026] The pretreatment water is fresh water and seawater in a volume ratio of 1:1. The washed algal body is dried with a water absorption paper.

[0027] S3: Completely wrap the algae in S2 with plastic wrap and irradiate the wrapped algae with 280-315 nanometer ultraviolet light for 8-10 minutes.

[0028] Completely wrap the algae with plastic wrap, ensuring the wrapping thickness does not exceed 5 layers, ideally 2 layers. Avoid excessive thickness, as this will affect the irradiation effect. During wrapping, do not damage the algae's original reproductive organs or basal holdfast. Place the algae on the support of the UV chamber and turn on the UV lamps to allow the UV light to sterilize the algae, maintaining a sterile environment as much as possible. The plastic wrap helps prevent rapid dehydration due to algal death and also prevents discoloration.

[0029] S4: Prepare the preservation solution by uniformly mixing and stirring sulfurous acid, glycerol and water in a volume ratio of 4:10:86 to obtain the preservation solution;

[0030] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0031] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens. Example 2:

[0032] The method for preparing primary color specimens of brown algae includes the following steps:

[0033] S1: Collect brown algal thallus with reproductive organs and basal holdfasts;

[0034] S2: Use pretreated water to wash the algae in S1, select algae with intact reproductive organs and basal holdfasts, and dry them.

[0035] The pretreatment water consisted of fresh water and seawater in a 1:1 volume ratio. After washing, the algae were dried with absorbent paper.

[0036] S3: Completely wrap the algae in S2 with plastic wrap and irradiate the wrapped algae with 280-315 nanometer ultraviolet light for 8 minutes.

[0037] Preferably, two layers of cling film are used.

[0038] S4: Prepare the preservation solution by uniformly mixing and stirring sulfurous acid, glycerol and water in a volume ratio of 4:10:86 to obtain the preservation solution;

[0039] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0040] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens. Example 3:

[0041] The method for preparing primary color specimens of brown algae includes the following steps:

[0042] S1: Collect brown algal thallus with reproductive organs and basal holdfasts;

[0043] S2: Use pretreated water to wash the algae in S1, select algae with intact reproductive organs and basal holdfasts, and dry them.

[0044] The pretreatment water consisted of fresh water and seawater in a 1:1 volume ratio. After washing, the algae were dried with absorbent paper.

[0045] S3: Completely wrap the algae in S2 with plastic wrap and irradiate the wrapped algae with 280-315 nanometer ultraviolet light for 9 minutes.

[0046] Preferably, two layers of cling film are used.

[0047] S4: Prepare the preservation solution by uniformly mixing and stirring sulfurous acid, glycerol and water in a volume ratio of 4:10:86 to obtain the preservation solution;

[0048] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0049] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens. Example 4:

[0050] The method for preparing primary color specimens of brown algae includes the following steps:

[0051] S1: Collect brown algal thallus with reproductive organs and basal holdfasts;

[0052] S2: Use pretreated water to wash the algae in S1, select algae with intact reproductive organs and basal holdfasts, and dry them.

[0053] The pretreatment water consisted of fresh water and seawater in a 1:1 volume ratio. After washing, the algae were dried with absorbent paper.

[0054] S3: Completely wrap the algae in S2 with plastic wrap, and irradiate the wrapped algae with 280-315 nanometer ultraviolet light for 10 minutes; preferably, use 2 layers of plastic wrap.

[0055] S4: Prepare the preservation solution by uniformly mixing and stirring sulfurous acid, glycerol and water in a volume ratio of 4:10:86 to obtain the preservation solution;

[0056] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0057] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens. Example 5:

[0058] The method for preparing primary color specimens of red algae includes the following steps:

[0059] S1: Collect red algal thallus with reproductive organs and basal holdfasts;

[0060] S2: Use pretreated water to wash the algae in S1, select algae with intact reproductive organs and basal holdfasts, and dry them.

[0061] The pretreatment water consisted of fresh water and seawater in a 1:1 volume ratio. After washing, the algae were dried with absorbent paper.

[0062] S3: Completely wrap the algae in S2 with plastic wrap, and irradiate the wrapped algae with 280-315 nanometer ultraviolet light for 8 minutes; preferably, 2 layers of plastic wrap.

[0063] S4: Prepare the preservation solution by uniformly mixing and stirring sulfurous acid, glycerol and water in a volume ratio of 4:10:86 to obtain the preservation solution;

[0064] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0065] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens. Example 6:

[0066] The method for preparing primary color specimens of red algae includes the following steps:

[0067] S1: Collect red algal thallus with reproductive organs and basal holdfasts;

[0068] S2: Use pretreated water to wash the algae in S1, select algae with intact reproductive organs and basal holdfasts, and dry them.

[0069] The pretreatment water consisted of fresh water and seawater in a 1:1 volume ratio. After washing, the algae were dried with absorbent paper.

[0070] S3: Completely wrap the algae in S2 with plastic wrap, and irradiate the wrapped algae with 280-315 nanometer ultraviolet light for 9 minutes; preferably, use 2 layers of plastic wrap.

[0071] S4: Prepare the preservation solution by uniformly mixing and stirring sulfurous acid, glycerol and water in a volume ratio of 4:10:86 to obtain the preservation solution;

[0072] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0073] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens. Example 7:

[0074] The method for preparing primary color specimens of red algae includes the following steps:

[0075] S1: Collect red algal thallus with reproductive organs and basal holdfasts;

[0076] S2: Use pretreated water to wash the algae in S1, select algae with intact reproductive organs and basal holdfasts, and dry them.

[0077] The pretreatment water consisted of fresh water and seawater in a 1:1 volume ratio. After washing, the algae were dried with absorbent paper.

[0078] S3: Completely wrap the algae in S2 with plastic wrap, and irradiate the wrapped algae with 280-315 nanometer ultraviolet light for 10 minutes; preferably, use 2 layers of plastic wrap.

[0079] S4: Prepare the preservation solution by uniformly mixing and stirring sulfurous acid, glycerol and water in a volume ratio of 4:10:86 to obtain the preservation solution;

[0080] S5: Immerse the algae from S3 in the preservation solution; remove after soaking for 5 minutes;

[0081] S6: Preparation of original color specimens. Take out the algae from S5 and place them in a paper bag. Place them in a dry and ventilated place to air dry naturally. After drying, they will be original color specimens.

[0082] Comparative Example 1:

[0083] S1: For the same brown algae, cut 4 segments and take pictures of these 4 segments with a camera, and record them as P1, P2, P3 and P4.

[0084] S2: Wrap each of the four brown algae segments in two layers of plastic wrap and irradiate them with 280-315 nanometer ultraviolet light for 8, 9, 10 and 11 minutes respectively. Then take photos of each of the four brown algae segments with a camera.

[0085] S3: Randomly select the RGB color components of 150 pixels in P1 and take the average value to obtain the RGB color space components in P1, denoted as (Ar1, Ag1, Ab1); similarly, obtain the RGB color space components in P2 and P3 respectively, denoted as (Ar2, Ag2, Ab2) and (Ar3, Ag3, Ab3); the RGB color space components in P4 are (Ar4, Ag4, Ab4).

[0086] S4: Using the method in S3, obtain the components in the RGB color space corresponding to 8, 9, 10 and 11 minutes in S2, respectively, and denoted as (Br1, Bg1, Bb1), (Br2, Bg2, Bb2), (Br3, Bg3, Bb3) and (Br4, Bg4, Bb4).

[0087] S5: Calculate the corresponding Euclidean distances respectively;

[0088] D1 = sqrt((Ar1 – Br1)^2 + (Ag1 – Bg1)^2 + (Ab1 – Bb1)^2);

[0089] D2= sqrt((Ar2 – Br2)^2 + (Ag2 – Bg2)^2 + (Ab2 – Bb2)^2);

[0090] D3= sqrt((Ar3 – Br3)^2 + (Ag3 – Bg3)^2 + (Ab3 – Bb3)^2);

[0091] D4= sqrt((Ar4 – Br4)^2 + (Ag4 – Bg4)^2 + (Ab4 – Bb4)^2);

[0092] Figure 1 The experiment showed that within 8-10 minutes, the Euclidean distance increased, indicating that the brown algae gradually faded in color during this period. However, the overall Euclidean distance value remained small, suggesting that the fading was not significant. At 11 minutes, the Euclidean distance value of the brown algae increased rapidly, indicating that the algae faded quickly and was no longer its original color.

[0093] Comparative Example 2:

[0094] S1: For the same red algae, cut 4 segments and take pictures of these 4 segments with a camera, and label them P1, P2, P3 and P4.

[0095] S2: Wrap each of the four red algae segments in two layers of plastic wrap and irradiate them with ultraviolet light for 8, 9, 10 and 11 minutes respectively. Then take photos of each of the four red algae segments with a camera.

[0096] S3: Randomly select the RGB color components of 150 pixels in P1 and take the average value to obtain the RGB color space components in P1, denoted as (Ar1, Ag1, Ab1); similarly, obtain the RGB color space components in P2 and P3 respectively, denoted as (Ar2, Ag2, Ab2) and (Ar3, Ag3, Ab3); the RGB color space components in P4 are (Ar4, Ag4, Ab4).

[0097] S4: Using the method in S3, obtain the components in the RGB color space corresponding to 8, 9, 10 and 11 minutes in S2, respectively, and denoted as (Br1, Bg1, Bb1), (Br2, Bg2, Bb2), (Br3, Bg3, Bb3) and (Br4, Bg4, Bb4).

[0098] S5: Calculate the corresponding Euclidean distances respectively;

[0099] D1 = sqrt((Ar1 – Br1)^2 + (Ag1 – Bg1)^2 + (Ab1 – Bb1)^2);

[0100] D2= sqrt((Ar2 – Br2)^2 + (Ag2 – Bg2)^2 + (Ab2 – Bb2)^2);

[0101] D3= sqrt((Ar3 – Br3)^2 + (Ag3 – Bg3)^2 + (Ab3 – Bb3)^2);

[0102] D4= sqrt((Ar4 – Br4)^2 + (Ag4 – Bg4)^2 + (Ab4 – Bb4)^2);

[0103] Figure 1 The experiment showed that within 8-10 minutes, the Euclidean distance increased, indicating that the red algae gradually faded in color during this period. However, the overall Euclidean distance value remained small, suggesting that the fading was not significant. At 11 minutes, the Euclidean distance value of the red algae increased rapidly, indicating that the algae faded quickly and was no longer its original color.

[0104] Based on the above-described preferred embodiments of the present invention, and through the foregoing description, those skilled in the art can make various changes and modifications without departing from the inventive concept. The technical scope of this invention is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. A method for making a large-sized seaweed original color specimen, characterized by, It comprises the following steps: S1: take the thallus with the reproductive organs and the basal fixer; S2: clean the thallus in S1 with pretreated water, select the thallus with the complete reproductive organs and the basal fixer, and suck the water dry; S3: completely wrap the thallus in S2 with the preservative film, and irradiate the wrapped thallus with ultraviolet rays of 280-315 nm; S4: configure the storage solution; the storage solution is uniformly mixed with sulfurous acid, glycerol and water in a volume ratio of 4:10:86; S5: immerse the thallus in S3 in the storage solution; take it out after soaking for 5 minutes; S6: make the original color specimen, take out the thallus in S5 and place it in a paper bag, place it in a dry and ventilated position and dry naturally, and it is the original color specimen after drying.

2. A method of preparing a large seaweed specimen in its natural color according to claim 1, comprising the steps of: In step S3, the irradiation time of the ultraviolet rays is 8-10 minutes.

3. A method of preparing a large seaweed specimen in its natural color according to claim 1, comprising the steps of: In step S3, the wrapping thickness of the preservative film is 2 layers.

4. A method of preparing a large seaweed specimen in its natural color according to claim 1, comprising the steps of: In step S2, the pretreated water is fresh water and seawater in a volume ratio of 1:1; the washed thallus is dried with a water-absorbing paper.

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