A pet diet agent based on hyperbranched polylysine-starch complex and a preparation method thereof

Abstract

The application discloses a pet diet agent based on hyperbranched polylysine-starch compound and a preparation method thereof, and the pet diet agent is composed of hyperbranched polylysine (HBPL) and starch. The hyperbranched polylysine (HBPL) and the starch generate the hyperbranched polylysine-starch compound through electrostatic interaction. The pet diet agent can effectively decompose emulsion containing cholate and phosphocholine, further inhibit lipase activity, reduce the absorption of fat in the small intestine, and achieve the effect of inhibiting pet obesity, and the polylysine-starch compound can significantly change the intestinal flora structure and improve the immunity of pets.

Description

Technical Field

[0001] This invention relates to a pet food supplement based on hyperbranched polylysine-starch complex and its preparation method, belonging to the field of pet health products. Background Technology

[0002] With rising living standards, people are paying increasing attention to pet health. Pet obesity is becoming increasingly common in today's society, negatively impacting not only their quality of life but also potentially leading to serious health risks, including joint diseases, diabetes, and heart disease. One of the main causes of pet obesity is excessive energy intake and insufficient exercise. During pet ownership, owners often struggle to control their pets' food intake and frequently choose high-energy, high-fat foods, resulting in excessive calorie consumption. Therefore, providing a dietary supplement that meets pets' basic nutritional needs while helping them control their weight is essential. Currently, several products exist on the market for managing pet weight, but their effectiveness and safety vary. Some products may rely too heavily on restricting food intake, neglecting to provide comprehensive nutrition. Other products may contain inappropriate additives or ingredients that could negatively affect pets' health.

[0003] Meanwhile, a pet's immunity is crucial to its overall health. The gut microbiota plays a vital role in a pet's immune system. Scientific research shows that the gut microbiota is closely related to a pet's immune system, and an imbalance in the microbiota can lead to a decline in immune system function, making pets more susceptible to various health problems.

[0004] This invention aims to address pet obesity and immune health issues through a safe, non-toxic, and effective pet diet, providing pet owners with a reliable method to help them maintain their pets' ideal weight and strengthen their immune system. Summary of the Invention

[0005] The purpose of this invention is to provide a pet dietary supplement with comprehensive effects, which can both inhibit pet obesity and significantly alter the structure of the pet's gut microbiota, thereby enhancing its immunity. This dietary supplement contains a series of natural, gut microbiota-friendly ingredients that help control the pet's weight while promoting the growth of beneficial bacteria and inhibiting the proliferation of harmful bacteria. Through daily dietary supplementation, pets can more easily maintain a healthy gut microbiota balance, thereby improving the vitality and resistance of their immune system.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A pet food supplement based on a hyperbranched polylysine-starch complex is disclosed. The pet food supplement is composed of hyperbranched polylysine (HBPL) and starch. The supplement can be directly added to pet food to promote weight loss and balance gut microbiota. The preparation method involves dissolving hyperbranched polylysine and starch in water (usually, starch is first dissolved in an aqueous solution, and after thorough mixing, hyperbranched polylysine is added and stirred until homogeneous). The mixture is stirred and reacted at room temperature for 12-24 hours. A pH adjuster is added to adjust the pH of the solution. After the reaction is complete, the solution is freeze-dried to obtain the hyperbranched polylysine-starch complex pet food supplement.

[0008] The solution is suitable for a pH range of 6-8.

[0009] The mass ratio of hyperbranched polylysine to starch is 1:100-1:500.

[0010] The electrostatic interaction is the interaction between positively charged hyperbranched polylysine and negatively charged starch.

[0011] The pH adjuster is one or more of citric acid, acetic acid, and phosphoric acid.

[0012] Beneficial effects of the present invention

[0013] 1. The hyperbranched polylysine-starch complex pet food preparation prepared by this invention can be directly added to pet food. It can effectively decompose emulsions containing bile salts and phosphocholine, thereby inhibiting lipase activity, reducing the absorption of fat in the small intestine, and achieving the effect of inhibiting pet obesity. Moreover, the polylysine-starch complex can significantly change the intestinal flora structure and enhance the pet's immunity.

[0014] 2. The hyperbranched polylysine-starch complex pet food supplement prepared by this invention has a very strong lipase inhibitory effect, thus requiring a smaller dosage.

[0015] 3. The preparation process of the hyperbranched polylysine-starch complex pet food preparation prepared by the present invention is simple, safe, and has no side effects. The degradation products, lysine and glucose, are essential for the human body, non-toxic, and beneficial to health. Detailed Implementation

[0016] The present invention will be further described in detail below with reference to specific examples.

[0017] Example 1

[0018] The preparation method of the pet food supplement in this embodiment includes the following steps:

[0019] Hyperbranched polylysine and starch were added to an appropriate amount of deionized water (the starch was dissolved first, then the hyperbranched polylysine was added). The mass ratio of hyperbranched polylysine to starch was 1:100. The mixture was stirred for 12 hours, and the pH was adjusted to 7.0 with citric acid. The mixture was then freeze-dried to obtain a hyperbranched polylysine-starch complex. One part hyperbranched polylysine-starch complex and 100 parts pet food were used. The above ingredients were weighed according to their respective weight parts and thoroughly mixed to obtain a pet weight-loss food.

[0020] Example 2

[0021] Hyperbranched polylysine and starch were added to an appropriate amount of deionized water (the starch was dissolved first, then the hyperbranched polylysine was added). The mass ratio of hyperbranched polylysine to starch was 1:200. The mixture was stirred for 12 hours, and the pH was adjusted to 6.0 with citric acid. The mixture was then freeze-dried to obtain a hyperbranched polylysine-starch complex. One part hyperbranched polylysine-starch complex and 50 parts pet food were used. The above ingredients were weighed according to their respective weight parts and thoroughly mixed to obtain a pet weight-loss food.

[0022] Example 3

[0023] Hyperbranched polylysine and starch were added to an appropriate amount of deionized water (the starch was dissolved first, then the hyperbranched polylysine was added). The mass ratio of hyperbranched polylysine to starch was 1:300. The mixture was stirred for 12 hours, and the pH was adjusted to 8.0 with citric acid. The mixture was then freeze-dried to obtain a hyperbranched polylysine-starch complex. One part hyperbranched polylysine-starch complex and 33 parts pet food were used. The above ingredients were weighed according to their respective weight parts and thoroughly mixed to obtain a pet weight-loss food.

[0024] Example 4

[0025] Hyperbranched polylysine and starch were added to an appropriate amount of deionized water (the starch was dissolved first, then the hyperbranched polylysine was added). The mass ratio of hyperbranched polylysine to starch was 1:400. The mixture was stirred for 18 hours, and the pH was adjusted to 6.5 with citric acid. The mixture was then freeze-dried to obtain a hyperbranched polylysine-starch complex. One part hyperbranched polylysine-starch complex and 25 parts pet food were used. The above ingredients were weighed according to their respective weight parts and thoroughly mixed to obtain a pet weight-loss food.

[0026] Example 5

[0027] Hyperbranched polylysine and starch were added to an appropriate amount of deionized water (the starch was dissolved first, then the hyperbranched polylysine was added). The mass ratio of hyperbranched polylysine to starch was 1:500. The mixture was stirred for 24 hours, and the pH was adjusted to 7.5 with citric acid. The mixture was then freeze-dried to obtain a hyperbranched polylysine-starch complex. One part hyperbranched polylysine-starch complex and 20 parts pet food were used. The above ingredients were weighed according to their respective weight parts and thoroughly mixed to obtain a pet weight-loss food.

[0028] Comparative Example 1

[0029] This comparative example is similar to Examples 1-5, except that hyperbranched polylysine-starch complex was removed from the pet food.

[0030] Thirty healthy adult beagles were fed a high-fat diet (crude protein ≥30%, crude fat ≥20%) for four consecutive weeks to induce a high-fat obesity model. After successful modeling, they were randomly divided into six groups of five healthy dogs each, based on their body weight. Five groups were fed pet food supplemented with the pet dietary additives from Examples 1-5, while the other group (Comparative Example 1) served as the control group, feeding only pet food without the additives. The dosage of the weight-loss composition in the pet food was 10 g / kg·BW / d in all groups, and the feeding methods were identical. The amount of basic dog food in the weight-loss diet was the same in all groups, and the feeding was continuous for eight weeks.

[0031] Weight was measured before the start of the experiment, after successful model establishment, and every four weeks during the experiment. The results are shown in Table 1.

[0032] Table 1. Effects of various pet dietary supplements on body weight.

[0033] Week 0 Week 4 Week 8 Week 12 Example 1 <![CDATA[10.23±1.21 a ]]> <![CDATA[12.87±1.68 b ]]> <![CDATA[11.46±1.20 b ]]> <![CDATA[10.16±1.90 a ]]> Example 2 <![CDATA[10.43±1.34 a ]]> <![CDATA[12.67±1.70 b ]]> <![CDATA[11.77±1.69 b ]]> <![CDATA[11.10±1.78 a ]]> Example 3 <![CDATA[10.35±1.67 a ]]> <![CDATA[12.86±1.43 b ]]> <![CDATA[11.80±1.67 b ]]> <![CDATA[10.93±1.59 a ]]> Example 4 <![CDATA[10.67±1.73 a ]]> <![CDATA[13.01±1.45 b ]]> <![CDATA[12.11±1.60 b ]]> <![CDATA[11.22±1.30 a ]]> Example 5 <![CDATA[10.56±1.25 a ]]> <![CDATA[12.78±1.78 b ]]> <![CDATA[11.87±1.56 b ]]> <![CDATA[11.13±1.70 a ]]> Comparative Example 1 <![CDATA[10.64±1.45 a ]]> <![CDATA[12.89±1.89 a ]]> <![CDATA[13.45±1.28 a ]]> <![CDATA[14.56±1.63 a ]]>

[0034] Note: a: No significant difference (P>0.05), b: Significant difference (P<0.05), pet weight is in kg.

[0035] As shown in Table 1:

[0036] 1. After 4 weeks of experimentation, the body weight of all participants increased significantly (P<0.05), indicating that the model was successfully established;

[0037] 2. The body weight of the pets in Examples 1-5 was significantly different from that in the fourth week of the experiment (P<0.05), indicating that the pet diets in Examples 1-5 have a good weight loss effect;

[0038] 3. There was no significant difference in body weight between Comparative Example 1 and Week 4 of the experiment (P>0.05). The weight increased without the addition of dietary supplements, indicating that adding dietary supplements to pet food has a significant weight loss effect.

[0039] Gut microbiota assay

[0040] Before feeding, the gut microbiota of 6 groups of healthy dogs was measured and the average value of the beneficial bacteria in each group was recorded. Then, after 4 weeks of the experiment, a second measurement was performed and recorded. After 8 weeks of the experiment, a third measurement was performed and recorded. After 12 weeks of the experiment, a fourth measurement was performed and recorded. The results are shown in Table 2.

[0041] Table 2. Bacterial counts at weeks 0, 4, 8, and 12 of the experiment.

[0042]

[0043] Note: a: P < 0.05 compared to Comparative Example 1.

[0044] The results in Table 2 show that after 4 weeks of the experiment, the number of beneficial bacteria in the pet's gut decreased to some extent. After 12 weeks of the experiment, the number of beneficial bacteria in the pet's gut increased after adding the hyperbranched polylysine-starch complex from Examples 1-5. This indicates that adding the hyperbranched polylysine-starch complex to pet food can protect the pet's gut health, stabilize the gut microbiota, promote the growth of beneficial bacteria, and thus strengthen the pet's immune system.

Claims

1. A pet food supplement based on hyperbranched polylysine-starch complex, characterized in that, The pet food supplement comprises hyperbranched polylysine (HBPL) and starch, and is a hyperbranched polylysine-starch complex formed by the electrostatic interaction of hyperbranched polylysine (HBPL) and starch. It is prepared by the following method: starch is dissolved in an aqueous solution, and after uniform mixing, hyperbranched polylysine is added and stirred until homogeneous. The hyperbranched polylysine-starch complex is then prepared by electrostatic interaction. The reaction is carried out at room temperature for 12-24 hours, and a pH adjuster is added. After the reaction is complete, the mixture is freeze-dried to obtain the pet food supplement. The mass ratio of hyperbranched polylysine to starch is 1:100-1:

500.

2. The pet food supplement according to claim 1, characterized in that, The electrostatic interaction is the interaction between positively charged hyperbranched polylysine and negatively charged starch.

3. The pet food supplement according to claim 1, characterized in that, The pH range of the solution is 6-8.

4. The pet food supplement according to claim 1, characterized in that, The pH adjuster is one or more of citric acid, acetic acid, and phosphoric acid.

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