Production method of medicinal plant additive for suckling pig feed containing vitamin B12

Abstract

The present invention discloses a method containing VB 12 The production method of the medicinal plant additive for suckling pig feed and the product thereof relate to the field of suckling pig breeding. The production method of the medicinal plant additive for suckling pig feed of the present invention comprises the following steps: (1) crushing cardamom, tricholoma, oregano and inulin respectively and enzymolyzing them with cellulase, and then mixing them in proportion, or first mixing cardamom, tricholoma, oregano and inulin in proportion, and then crushing and enzymolyzing them with cellulase to obtain a primary enzymolysis material; (2) adding potassium dihydrogen phosphate and oxalic acid to the primary enzymolysis material, and continuing to degrade to obtain a hot-cooked enzymolysis material; (3) mixing the hot-cooked enzymolysis material with molasses, wormwood, glucose, and yeast extract to obtain a mixture, adding sterilized tap water and Clostridium butyricum liquid, and fermenting to obtain a primary fermentation material; (4) adding pretreated Sanguisorba officinalis and molasses to the primary fermentation material, and continuing to ferment. The medicinal plant additive for suckling pig feed of the present invention can significantly reduce the feed-to-meat ratio of suckling pigs and significantly increase daily weight gain.

Description

Technical Field

[0001] The present invention relates to the technical field of suckling pig breeding, and more particularly to a method of feeding a suckling pig comprising VB 12 The invention discloses a production method of a medicinal plant additive for suckling pig feed and its product. Background Art

[0002] In the past, antibiotics played a role in disease prevention and control and growth promotion in the aquaculture industry. With the use of antibiotics and the further development of science and technology, studies have found that the use of antibiotics has caused some bacterial resistance problems, which in turn has brought some food safety risks.

[0003] Therefore, how to provide an alternative product or method to enhance immunity and promote growth in the aquaculture industry is an urgent problem that needs to be solved by those skilled in the art. Summary of the Invention

[0004] In view of this, the present invention provides a fermentation method for producing a high vitamin B12 product by using Chinese medicine mugwort as the main raw material, compounding with cardamom, tricholoma, oregano and elecampane. 12 The medicinal plant additives for suckling pig feed are used to improve the immunity of suckling pigs and promote their growth.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A VB-containing 12 The method for producing a medicinal plant additive for suckling pig feed comprises the following steps:

[0007] (1) pulverizing cardamom, striga, oregano, and elecampane in sequence, and performing enzymolysis with cellulase, and then mixing them in proportion; or pulverizing cardamom, striga, oregano, and elecampane in advance, and then performing enzymolysis with cellulase, to obtain a primary enzymolysis material;

[0008] (2) adding potassium dihydrogen phosphate and oxalic acid to the primary enzymatic hydrolysis material and continuing the degradation to obtain a hot-cooked enzymatic hydrolysis material;

[0009] (3) mixing the hot-cooked enzymatic hydrolyzed material with molasses, mugwort leaves, glucose, and yeast extract to obtain a mixture, adding sterilized tap water and Clostridium butyricum liquid, and fermenting to obtain a primary fermentation material;

[0010] (4) Pretreated sanguinea vine and molasses are added to the primary fermentation material, and the fermentation is continued to obtain a medicinal plant additive for suckling pig feed.

[0011] Vitamin B 12It plays an important role in the growth of suckling pigs. At the same time, the effective ingredients in the traditional Chinese medicine and Clostridium butyricum also have an important impact on the growth and metabolism of suckling pigs. When the traditional Chinese medicine of the present invention is fermented, the ingredients of the traditional Chinese medicine will affect the metabolism of the bacteria, thereby making the entire fermentation system more conducive to the growth of suckling pigs and the improvement of immunity.

[0012] As a preferred technical solution of the present invention, the ratio in step (1) is a mass ratio, specifically cardamom: tricholoma: oregano: elecampane = 1:2:3:1.

[0013] As a preferred technical solution of the present invention, the pulverization in step (1) is 100 mesh;

[0014] The conditions for the cellulase hydrolysis are: keeping warm at 50° C. for 16 hours, and adding cellulase in an amount of 1% of the mass of the material to be hydrolyzed, and adding sterilized tap water in an amount of 36% of the mass of the material to be hydrolyzed.

[0015] As a preferred technical solution of the present invention, the amount of potassium dihydrogen phosphate added in step (2) is 0.01% of the mass of the primary enzymatic hydrolysis material, and the amount of oxalic acid added is 0.06% of the mass of the primary enzymatic hydrolysis material;

[0016] Furthermore, the degradation was continued under the condition of keeping the temperature at 70° C. for 10 hours. The mass concentration of the oxalic acid was 36%.

[0017] As a preferred technical solution of the present invention, the mass ratio of the hot-cooked enzymatic hydrolysis material to molasses, mugwort leaves, glucose, and yeast extract in step (3) is 18:20:80:3:2;

[0018] The amount of the sterilized tap water added is 30% of the mass of the mixture, and the amount of the Clostridium butyricum liquid added is 2% of the mass of the mixture;

[0019] Furthermore, the mugwort leaves are crushed and passed through a 100-mesh sieve.

[0020] As a preferred technical solution of the present invention, the thickness of the fermentation material layer in step (3) is controlled to be 25 cm, the fermentation temperature is 30° C., and the fermentation time is 20 h.

[0021] As a preferred technical solution of the present invention, the preparation method of Clostridium butyricum liquid is as follows: from the Clostridium butyricum species preserved on the slant, six rings of bacteria are scraped with an inoculating loop (loop diameter 0.3 mm), inoculated into 300 mL of culture medium (1% wt yeast extract, 1% wt peptone, 4% wt glucose; after preparation, sterilized at 115°C for 30 minutes, cooled for standby use), loaded into a 500 mL Erlenmeyer flask, cultured at 33°C, a rotation speed of 180 r / m, and cultured for 18 hours to obtain the Clostridium butyricum liquid.

[0022] As a preferred technical solution of the present invention, the mass ratio of the primary fermentation material to the pretreated Saccharum officinale and molasses in step (4) is 85:5:10.

[0023] As a preferred technical solution of the present invention, the thickness of the material layer for continued fermentation in step (4) is 15 cm, the fermentation temperature is 30° C., and the fermentation time is 16 h.

[0024] As a preferred technical solution of the present invention, the pretreatment method of the safflower vine in step (4) is to acid-hydrolyze the safflower vine with a 5% oxalic acid solution whose mass is 4 times that of the safflower vine, acid-hydrolyze at 50°C for 12 hours, and then keep it at 65°C for 16 hours to dry the safflower vine.

[0025] Another object of the present invention is to provide a VB-containing product prepared by any of the above production methods. 12 Medicinal plant additive for suckling pig feed.

[0026] The invention has the beneficial effects of significantly reducing the feed-to-meat ratio of suckling pigs and significantly increasing the daily weight gain. DETAILED DESCRIPTION

[0027] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0028] The embodiment of the present invention discloses a method containing VB 12 The invention relates to a method for producing a medicinal plant additive for suckling pig feed and a product thereof, wherein the raw materials used are all commercially available and their sources are not specifically limited, such as Clostridium butyricum (Wei Lan Biotechnology), peptone (Jinan Lan Jue Trading Co., Ltd.), glucose (Shandong Xujie Chemical Co., Ltd.), cellulase (Xiasheng Group, cellulase CEL-01, 11000U / g), molasses (Jinan Hongtai Chemical Co., Ltd.), yeast extract (Jinan Xinsenyuan Chemical Co., Ltd.), Qi Ai Artemisia argyi (Ding Ai Technology), cardamom (Bozhou Renyi Chinese Medicine Sales Co., Ltd.), blood wind vine (Bozhou Yihongtang Pharmaceutical Co., Ltd.), single foot gold (Anhui Bozhou Medicine General Company Medicinal Materials Company), oregano (Leling Yongxinghe Food Co., Ltd.), elecampane (Bozhou Chen Chunmei Pharmaceutical Co., Ltd.), potassium dihydrogen phosphate (Xingtai Chemical), oxalic acid (Liangtai Chemical, mass concentration 36%), vitamin B 12 Standards (Aladdin), sodium nitrite (Nanjing Reagent), glacial acetic acid (Nanjing Reagent), sodium cyanide (Nanjing Reagent), KH2PO4 (Foshan Shunde Weima Chemical Co., Ltd.), acetonitrile (Aladdin).

[0029] Unless otherwise mentioned, the methods involved are conventional methods and will not be described in detail here.

[0030] Example 1

[0031] Preparation of medicinal plant additives for suckling pig feed:

[0032] (1) Grind cardamom and pass it through a 100-mesh sieve. Add 1% cellulase (11,000 U / g) based on the weight of the cardamom powder, add 36% sterilized tap water, and keep warm at 50°C for 16 hours to obtain the cardamom enzymatic hydrolyzate.

[0033] The Striga was crushed and passed through a 100-mesh sieve. Based on the mass of the Striga powder, 1% cellulase (11000 U / g) was added, and 36% sterilized tap water was added. The mixture was kept at 50°C for 16 hours to obtain the Striga enzymatic hydrolyzed material.

[0034] The oregano was crushed and passed through a 100-mesh sieve. Based on the mass of the oregano powder, 1% cellulase (11000 U / g) was added, and 36% sterilized tap water was added. The mixture was kept at 50°C for 16 hours to obtain an oregano enzymatic hydrolyzate.

[0035] The inula was crushed and passed through a 100-mesh sieve. Based on the mass of the inula powder, 1% cellulase (11000 U / g) was added, and 36% sterilized tap water was added. The mixture was kept at 50°C for 16 hours to obtain the inula enzymatic hydrolyzate.

[0036] The cardamom enzymatic hydrolysis material, the Striga enzymatic hydrolysis material, the oregano enzymatic hydrolysis material and the Inula enzymatic hydrolysis material are uniformly mixed in a mass ratio of 1:2:3:1 to obtain a primary enzymatic hydrolysis material.

[0037] (2) Heat-ripening treatment: Add potassium dihydrogen phosphate and oxalic acid solution to the primary enzymatic hydrolysate and continue to degrade (keep at 70°C for 10 hours) to obtain heat-ripened enzymatic hydrolysate;

[0038] Among them, the addition amount of potassium dihydrogen phosphate is 0.01% of the mass of the primary enzymatic hydrolysis material, the addition amount of oxalic acid solution is 0.06% of the mass of the primary enzymatic hydrolysis material, and the mass concentration of oxalic acid is 36%.

[0039] (3) The hot-cooked enzymatic hydrolyzed material was mixed with molasses, mugwort, glucose, and yeast extract in a mass ratio of 18:20:80:3:2 to obtain a mixture, 30% of the mass of the mixture was sterilized tap water and 2% of Clostridium butyricum solution were added, and the mixture was spread and fermented with a material layer thickness of 25 cm and fermented at 30° C. for 20 h to obtain a primary fermentation material;

[0040] Among them, the mugwort is Qi Ai mugwort, which is pre-treated by crushing and passing through a 100-mesh sieve.

[0041] (4) adding pretreated Caulis Sanguisorba officinalis and molasses to the primary fermentation material (the mass ratio of the primary fermentation material to the pretreated Caulis Sanguisorba officinalis and molasses is 85:5:10), continuing the fermentation, controlling the material layer thickness to 15 cm, the fermentation temperature to 30° C., and the fermentation time to 16 h, to obtain a medicinal plant additive for suckling pig feed;

[0042] The pretreatment method of the Caulis Sanguisorbae was as follows: the Caulis Sanguisorbae was soaked in 5% oxalic acid solution at 50° C. for 12 h, then dried, and then kept warm at 65° C. for 16 h.

[0043] Comparative Example 1

[0044] In the raw materials, the Inula was replaced by Artemisia argyi of equal mass, and the rest was the same as in Example 1.

[0045] Comparative Example 2

[0046] The Caulis Sanguineae Rhizoma was not pretreated and was directly mixed with the primary fermentation material and molasses for fermentation, and the other procedures were the same as in Example 1.

[0047] Comparative Example 3

[0048] During the heat treatment, only oxalic acid was added to the primary enzymatic hydrolyzed material (the amount of oxalic acid solution added was 0.06% of the mass of the primary enzymatic hydrolyzed material, and the mass concentration of oxalic acid was 36%), and the rest was the same as in Example 1.

[0049] Comparative Example 4

[0050] The raw materials were replaced by wormwood leaves of equal mass, and the rest were the same as in Example 1.

[0051] Comparative Example 5

[0052] The enzymatic hydrolyzed powder of cardamom, enzymatic hydrolyzed powder of Striga, enzymatic hydrolyzed powder of oregano, and enzymatic hydrolyzed powder of Inula were mixed in a ratio of 1:2:3:1 to obtain a mixed enzymatic hydrolyzed powder (not subjected to heat cooking). The same procedures as in Example 1 were applied except that molasses: Artemisia argyi leaf: glucose: yeast extract: mixed enzymatic hydrolyzed powder were mixed in a mass ratio of 20:80:3:2:18.

[0053] Comparative Example 6

[0054] In addition to adding pretreated Caulis Sanguisorba officinalis and molasses into the primary fermentation material (the mass ratio of primary fermentation material to pretreated Caulis Sanguisorba officinalis and molasses was 85:5:10), the fermentation was not continued after mixing, and the VB was detected. 12 Content, add VB 12 260μg / kg, VB 12 Except that the content reached 426 μg / kg, other aspects were the same as those in Example 1.

[0055] Effect Example 1

[0056] Detection of VB in the medicinal plant additives for suckling pig feed obtained in Example 1 and Comparative Examples 1-512 content.

[0057] Sample processing: The sample was diluted 50 times with sterilized ultrapure water, shaken for 15 minutes after dilution, centrifuged at 3500 rpm for 10 minutes, and filtered with a microporous membrane.

[0058] Detection method: refer to the literature (Gu Qing; Li Ping; Sun Yalian; Yu Yue, High-yield folic acid and vitamin B 12 Research on lactic acid bacteria and their application in fermented milk, Scientific and Technological Achievements, 2016).

[0059] The test results are shown in Table 1:

[0060] Table 1

[0061]

[0062] Effect Example 2

[0063] Effects of the medicinal plant additives for suckling pig feed obtained in Example 1 and Comparative Examples 1-5 on the growth performance of suckling pigs

[0064] Method: Except that the addition amount of medicinal plant additives in piglet feed was 0.15%wt in the experimental group, other operations were the same as those in the literature (Xie Bifeng, Chen Chengwen, Chen Rubi. Feeding effect of Aspergillus niger SL2-111 feed compound enzyme on weaned piglets, Feed Expo, 2006).

[0065] Test results:

[0066] Example 1: The results of 21 days of feeding showed that the feed-to-meat ratio of the experimental group was reduced by 32.9% compared with the blank control group, and the daily weight gain of the experimental group was increased by 30.6% compared with the blank control group.

[0067] Comparative Example 1: The results of 21 days of feeding showed that the feed-to-meat ratio of the experimental group was 26.9% lower than that of the blank control group, and the daily weight gain of the experimental group was 27.6% higher than that of the blank control group.

[0068] Comparative Example 2: The results of 21 days of feeding showed that the feed-to-meat ratio of the experimental group was 23.9% lower than that of the blank control group, and the daily weight gain of the experimental group was 24.1% higher than that of the blank control group.

[0069] Comparative Example 3: The results of 21 days of feeding showed that the feed-to-meat ratio of the experimental group was 24.8% lower than that of the blank control group, and the daily weight gain of the experimental group was 25.6% higher than that of the blank control group.

[0070] Comparative Example 4: The results of 21 days of feeding showed that the feed-to-meat ratio of the experimental group was 29.1% lower than that of the blank control group, and the daily weight gain of the experimental group was 26.9% higher than that of the blank control group.

[0071] Comparative Example 5: The results of 21 days of feeding showed that the feed-to-meat ratio of the experimental group was 25.1% lower than that of the blank control group, and the daily weight gain of the experimental group was 26.3% higher than that of the blank control group.

[0072] Comparative Example 6: The results of 21 days of feeding showed that the feed-to-meat ratio of the experimental group was 26.1% lower than that of the blank control group, and the daily weight gain of the experimental group was 26.3% higher than that of the blank control group.

[0073] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0074] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A kind of VB 12 The method for producing a medicinal plant additive for suckling pig feed is characterized in that: The following steps are involved: (1) Crush cardamom, striga, oregano and elecampane in sequence and enzymatically hydrolyze with cellulase, and then mix them in proportion; or first mix cardamom, striga, oregano and elecampane in proportion, and then crush and enzymatically hydrolyze with cellulase to obtain a primary enzymatic hydrolyzate; The ratio described in this step is the mass ratio, specifically cardamom: striga: oregano: elecampane = 1:2:3:1; (2) Potassium dihydrogen phosphate and oxalic acid are added to the primary enzymatic hydrolysis material and the degradation is continued to obtain a hot-cooked enzymatic hydrolysis material; (3) mixing the hot-cooked enzymatic hydrolyzed material with molasses, mugwort leaves, glucose, and yeast extract to obtain a mixture, adding sterilized tap water and Clostridium butyricum liquid, and fermenting to obtain a primary fermentation material; The mass ratio of the hot-cooked enzymatic hydrolyzed material to molasses, wormwood leaf, glucose, and yeast extract is 18:20:80:3:2; (4) adding pretreated Sanguisorba officinalis and molasses to the primary fermentation material and continuing the fermentation to obtain a medicinal plant additive for suckling pig feed; The mass ratio of the primary fermentation material to the pretreated safflower vine and molasses is 85:5:10; and the pretreatment method of the safflower vine is to acidify the safflower vine with a 5% oxalic acid solution with a mass four times that of the safflower vine, acidify it at 50°C for 12 hours, and then keep it at 65°C for 16 hours to dry the safflower vine.

2. A VB-containing compound according to claim 1 12 The method for producing a medicinal plant additive for suckling pig feed is characterized in that: The crushing in step (1) is 100 mesh; The conditions for the cellulase hydrolysis are: keeping warm at 50° C. for 16 hours, and adding cellulase in an amount of 1% of the mass of the material to be hydrolyzed, and adding sterilized tap water in an amount of 36% of the mass of the material to be hydrolyzed.

3. A VB-containing compound according to claim 1 12 The method for producing a medicinal plant additive for suckling pig feed is characterized in that: In step (2), the amount of potassium dihydrogen phosphate added is 0.01% of the mass of the primary enzymatic hydrolysis material, and the amount of oxalic acid added is 0.06% of the mass of the primary enzymatic hydrolysis material; Furthermore, the condition for continued degradation is keeping the temperature at 70°C for 10 hours.

4. A VB-containing compound according to claim 1 12 The method for producing a medicinal plant additive for suckling pig feed is characterized in that: In step (3), the amount of the sterilized tap water added is 30% of the mass of the mixture, and the amount of the Clostridium butyricum liquid added is 2% of the mass of the mixture; Furthermore, the mugwort leaves are crushed and passed through a 100-mesh sieve.

5. A VB-containing compound according to claim 1 12 The method for producing a medicinal plant additive for suckling pig feed is characterized in that: In step (3), the thickness of the fermentation material layer is controlled to be 25 cm, the fermentation temperature is 30° C., and the fermentation time is 20 h.

6. A VB-containing compound according to claim 1 12 The method for producing a medicinal plant additive for suckling pig feed is characterized in that: The thickness of the material layer for continued fermentation in step (4) is 15 cm, the fermentation temperature is 30° C., and the fermentation time is 16 h.

7. The VB-containing product prepared by the production method according to any one of claims 1 to 6 12 Medicinal plant additive for suckling pig feed.

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