A composition with beauty effects and its application in oral preparations

Abstract

The present invention relates to the technical field of food processing, and particularly relates to an application of a composition for improving chloasma and its oral preparation in health food. The composition for improving chloasma comprises the following raw materials in parts by weight: 25-60 parts of hawthorn, 4-12 parts of mulberry leaves, 1-6 parts of phyllanthus emblica, 0.1-1.2 parts of chrysanthemum, 0.5-5 parts of kudzu root, 3-12 parts of Chinese yam, 1-8 parts of poria cocos, 0.1-1 part of ginseng, 0.05-0.9 part of donkey-hide gelatin, 0.1-1 part of mangosteen, and 40-100 parts of collagen peptide. Preparing health food with the composition for improving chloasma of the present invention has the efficacy of improving chloasma, is safe and convenient, and has a broad market prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of food processing, and particularly to the application of a composition for improving chloasma and its oral preparation in health foods. Background Art

[0002] The pursuit of fair, smooth and bright skin has always been the skin care principle of Orientals. The modern lifestyle features overly refined diet, reduced physical activity, overeating, or excessive fatigue, which gradually weakens the body's metabolic function, leads to disharmony of zang-fu organs, and easily causes pigmentation skin problems such as facial chloasma and freckles. Western medicine treatment mainly involves topical or oral administration of vitamins and flavonoids, but the effects vary, it is not easy to cure completely, and some drugs have relatively large toxic and side effects. Summary of the Invention

[0003] The purpose of the present invention is to provide a composition containing hawthorn, mulberry leaf, phyllanthus emblica, chrysanthemum, kudzu root, Chinese yam, poria cocos, ginseng, mangosteen, collagen peptide and donkey-hide gelatin. This composition works synergistically and has a significant effect on improving chloasma.

[0004] In order to achieve the above object of the present invention, the following technical solutions are provided:

[0005] A composition for improving chloasma, comprising the following raw materials in parts by weight: 25 - 60 parts of hawthorn, 4 - 12 parts of mulberry leaf, 1 - 6 parts of phyllanthus emblica, 0.1 - 1.2 parts of chrysanthemum, 0.5 - 5 parts of kudzu root, 3 - 12 parts of Chinese yam, 1 - 8 parts of poria cocos, 0.1 - 1 part of ginseng, 0.05 - 0.9 part of donkey-hide gelatin, 0.1 - 1 part of mangosteen, and 40 - 100 parts of collagen peptide.

[0006] Further, the composition comprises the following raw materials in parts by weight: 35 - 55 parts of hawthorn, 6 - 10 parts of mulberry leaf, 2 - 4 parts of phyllanthus emblica, 0.3 - 1 part of chrysanthemum, 1 - 3 parts of kudzu root, 4 - 9 parts of Chinese yam, 2 - 6 parts of poria cocos, 0.2 - 0.8 part of ginseng, 0.1 - 0.7 part of donkey-hide gelatin, 0.3 - 0.9 part of mangosteen, and 50 - 90 parts of collagen peptide.

[0007] Still further, the composition comprises the following raw materials in parts by weight: 40 parts of hawthorn, 9 parts of mulberry leaf, 3 parts of phyllanthus emblica, 0.5 part of chrysanthemum, 2.5 parts of kudzu root, 7 parts of Chinese yam, 4 parts of poria cocos, 0.5 part of ginseng, 0.4 part of donkey-hide gelatin, 0.5 part of mangosteen, and 70 parts of collagen peptide.

[0008] The collagen peptide is deep-sea fish collagen peptide or freshwater fish collagen peptide.

[0009] The chrysanthemum is selected from Hangzhou white chrysanthemum, Huangshan tribute chrysanthemum, Bozhou chrysanthemum, and Chuzhou chrysanthemum; preferably, the chrysanthemum is Hangzhou white chrysanthemum.

[0010] The present invention provides a method for preparing the above composition, which specifically includes the following steps:

[0011] (1) Decoct the formula amounts of hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen with 400 - 800 mL of water twice, filter, and combine the filtrates;

[0012] (2) Add the formula amounts of collagen peptide and donkey-hide gelatin to the combined filtrate, add water again and make up the volume to 1300 - 1700 mL;

[0013] (3) Can and sterilize the solution after volume adjustment, and then subpackage it.

[0014] In the step (1), the ratio of the mass of mangosteen added to the volume of water added is (0.1 - 1): 1.2 g / L.

[0015] In the step (2), the ratio of the mass of collagen peptide added to the volume of water added for volume adjustment is (40 - 100): 1.5 g / L.

[0016] The present invention provides an oral preparation, which contains the above composition.

[0017] The oral preparation further contains food-acceptable additives; the food-acceptable additives include 0.1 - 2 parts of acidulant and 20 - 70 parts of sweetener; preferably, the food-acceptable additives include 0.5 - 1.5 parts of acidulant and 30 - 60 parts of sweetener; in a specific embodiment, the food-acceptable additives include 1 part of acidulant and 45 parts of sweetener.

[0018] The sweetener is selected from one or more of xylitol, sorbitol, mannitol, lactitol, maltitol, isomaltitol, erythritol, stevioside, licorice, miraculin, mogroside, thaumatin; preferably, the sweetener is selected from one or more of xylitol, sorbitol, erythritol, stevioside.

[0019] The acidulant is selected from one or more of citric acid, ascorbic acid, gluconic acid, malic acid, tartaric acid, lactic acid, fumaric acid; preferably, the sweetener is selected from one or more of xylitol, sorbitol, erythritol, stevioside; in a specific embodiment, the acidulant is selected from one or more of ascorbic acid, gluconic acid, malic acid.

[0020] According to an embodiment of the present invention, the oral preparation is a powder, granule, oral liquid, capsule or tablet.

[0021] According to an embodiment of the present invention, when the oral preparation is an oral liquid, its preparation method includes the following steps:

[0022] (1) Decoct the medicinal amounts of hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen with 400 - 800 mL of water twice, filter, and combine the filtrates;

[0023] (2) Add the medicinal amounts of collagen peptide, sweetener, acidulant, and donkey-hide gelatin to the combined filtrate, add water again, and make up the volume to 1300 - 1700 mL;

[0024] (3) Sterilize and fill the volume-fixed solution into cans and sub-pack it to obtain the oral liquid.

[0025] The ratio of the mass of mangosteen added in step (1) to the volume of water added is (0.1 - 1): 1.2 g / L.

[0026] The ratio of the mass of collagen peptide added in step (2) to the volume of water added for volume fixation is (40 - 100): 1.5 g / L.

[0027] According to an embodiment of the present invention, when the oral preparation is a powder, its preparation method includes the following steps:

[0028] (1) Decoct the medicinal amounts of hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen with 400 - 800 mL of water twice, filter, and combine the filtrates, concentrate, dry, and pulverize to obtain the extract solid powder;

[0029] (2) Mix the extract solid powder evenly with the medicinal amounts of collagen peptide, sweetener, acidulant, and donkey-hide gelatin, and then sub-pack to obtain the powder.

[0030] The ratio of the mass of mangosteen added in step (1) to the volume of water added is (0.1 - 1): 1.2 g / L.

[0031] According to a preferred embodiment of the present invention, when the oral preparation is a granule, it includes the following steps:

[0032] (1) Decoct the medicinal amounts of hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen with 400 - 800 mL of water twice, filter, and combine the filtrates, and concentrate to obtain the extract;

[0033] (2) Add the medicinal amounts of collagen peptide, sweetener, acidulant, and donkey-hide gelatin to the extract, and spray granulate to obtain the granules.

[0034] The ratio of the mass of mangosteen added in step (1) to the volume of water added is (0.1 - 1): 1.2 g / L.

[0035] According to an embodiment of the present invention, when the oral preparation is a tablet, its preparation method includes the following steps:

[0036] (1) Decoct the formula amounts of hawthorn, Phyllanthus emblica, chrysanthemum, mulberry leaf, Poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen with 400 - 800 mL of water twice, filter, combine the filtrates, and concentrate to obtain an extract.

[0037] (2) Add the formula amounts of collagen peptide, sweetener, acidulant, and donkey-hide gelatin to the extract, perform spray granulation, and then press into tablets to obtain tablets.

[0038] The ratio of the mass of mangosteen added in step (1) to the volume of water added is (0.1 - 1):1.2 g / L.

[0039] According to an embodiment of the present invention, when the oral preparation is a capsule, its preparation method includes the following steps:

[0040] (1) Decoct the formula amounts of hawthorn, Phyllanthus emblica, chrysanthemum, mulberry leaf, Poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen with 400 - 800 mL of water twice, filter, combine the filtrates, and concentrate to obtain an extract.

[0041] (2) Add the formula amounts of collagen peptide, sweetener, acidulant, and donkey-hide gelatin to the extract, perform spray granulation, and then fill into capsules to obtain capsules.

[0042] The ratio of the mass of mangosteen added in step (1) to the volume of water added is (0.1 - 1):1.2 g / L.

[0043] The present invention provides the use of the above composition and its oral preparation in the preparation of health foods having the efficacy of improving chloasma.

[0044] The composition in the present invention can be used as a main raw material in the preparation of health foods having the efficacy of improving chloasma.

[0045] The so-called improvement of chloasma refers to the prevention, alleviation, or elimination of chloasma.

[0046] The beneficial effects of the present invention are as follows:

[0047] The present invention combines and proportionally mixes a variety of foods homologous to medicine and food with collagen peptide, having a high taste acceptance, and the taste is better after adding appropriate food additives. Each component works synergistically. Volunteers evaluated the freckle-removing effect of the product of the present invention, and the effective rate reached more than 70%. The oral liquid preparation of the composition of the present invention can inhibit the proliferation activity of B16 cells, reduce the local skin melanin deposition caused by the transfer of melanin to epidermal basal cells, thereby reducing the formation of skin spots, and can play a role in fading various different types of skin spots in a short time by treating both the symptoms and the root causes, with an obvious effect compared to the same type of oral liquid. Detailed implementation manners

[0048] The present invention will be further described by specific implementation methods below, but the application scope of the present invention is not limited to the following examples. Substitutions and / or combinations of the technical features of the present invention are obvious to those skilled in the art within the content, spirit, and / or scope of the present invention and are included in the present invention.

[0049] The hawthorn, mulberry leaf, phyllanthus emblica, chrysanthemum morifolium, kudzu root, Chinese yam, poria cocos, ginseng, mangosteen, and food additives used in the present invention are common commercially available products and can all be purchased in the market. The collagen peptide is deep-sea fish collagen peptide or freshwater fish collagen peptide, and the donkey-hide gelatin is purchased from the Dong'e area in Shandong. The amount of water added for decocting and the fixed volume in the preparation method of the present invention belong to conventional technologies, and the liquid concentration can be within the conventional range of oral preparations.

[0050] Example 1

[0051] A composition with the efficacy of improving chloasma, the components are as follows:

[0052] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of chrysanthemum morifolium, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, and 70 g of collagen peptide.

[0053] Preparation method:

[0054] (1) Decoct 40 g of hawthorn, 3 g of phyllanthus emblica, chrysanthemum morifolium, 9 g of mulberry leaf, 4 g of poria cocos, 7 g of Chinese yam, 0.5 g of ginseng, 2.5 g of kudzu root, and 0.5 g of mangosteen with 600 mL of water twice, filter, and combine the filtrates;

[0055] (2) Add the formula amount of collagen peptide and donkey-hide gelatin to the combined filtrate, add water again and make the volume up to 1500 mL;

[0056] (3) Can and sterilize the fixed-volume solution, and then subpackage it.

[0057] Example 2

[0058] A composition with the efficacy of improving chloasma, the components are as follows:

[0059] 50 g of hawthorn, 7.5 g of mulberry leaf, 2.5 g of phyllanthus emblica, 0.8 g of chrysanthemum morifolium, 1.6 g of kudzu root, 6 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.2 g of donkey-hide gelatin, 0.7 g of mangosteen, and 80 g of collagen peptide.

[0060] The preparation method is the same as that of Example 1.

[0061] Example 3

[0062] A composition with the efficacy of improving chloasma, the components are as follows:

[0063] 35 g of hawthorn, 10 g of mulberry leaf, 2 g of phyllanthus emblica, 0.3 g of hangzhou white chrysanthemum, 3 g of kudzu root, 9 g of Chinese yam, 2 g of poria cocos, 0.8 g of ginseng, 0.1 g of donkey-hide gelatin, 0.9 g of mangosteen, 90 g of collagen peptide.

[0064] The preparation method is the same as that of Example 1.

[0065] Example 4

[0066] A composition with the efficacy of improving chloasma, the components are as follows:

[0067] 35 g of hawthorn, 10 g of mulberry leaf, 2 g of phyllanthus emblica, 0.3 g of huangshan tribute chrysanthemum, 3 g of kudzu root, 9 g of Chinese yam, 2 g of poria cocos, 0.8 g of ginseng, 0.1 g of donkey-hide gelatin, 0.9 g of mangosteen, 90 g of collagen peptide.

[0068] The preparation method is the same as that of Example 1.

[0069] Example 5

[0070] A composition with the efficacy of improving chloasma, the components are as follows:

[0071] 55 g of hawthorn, 6 g of mulberry leaf, 4 g of phyllanthus emblica, 1 g of hangzhou white chrysanthemum, 1 g of kudzu root, 4 g of Chinese yam, 6 g of poria cocos, 0.2 g of ginseng, 0.7 g of donkey-hide gelatin, 0.3 g of mangosteen, 50 g of collagen peptide.

[0072] The preparation method is the same as that of Example 1.

[0073] Example 6

[0074] A composition with the efficacy of improving chloasma, the components are as follows:

[0075] 25 g of hawthorn, 12 g of mulberry leaf, 1 g of phyllanthus emblica, 0.1 g of hangzhou white chrysanthemum, 5 g of kudzu root, 3 g of Chinese yam, 8 g of poria cocos, 0.1 g of ginseng, 0.9 g of donkey-hide gelatin, 0.1 g of mangosteen, 40 g of collagen peptide.

[0076] The preparation method is the same as that of Example 1.

[0077] Example 7

[0078] A composition with the efficacy of improving chloasma, the components and its preparation method are as follows:

[0079] 60 g of hawthorn, 4 g of mulberry leaf, 6 g of phyllanthus emblica, 1.2 g of hangzhou white chrysanthemum, 0.5 g of kudzu root, 12 g of Chinese yam, 1 g of poria cocos, 1 g of ginseng, 0.05 g of donkey-hide gelatin, 1 g of mangosteen, 100 g of collagen peptide.

[0080] The preparation method is the same as that of Example 1.

[0081] Example 8

[0082] An oral liquid with the efficacy of improving chloasma, and the components are as follows:

[0083] 40 g of hawthorn, 9 g of mulberry leaves, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 45 g of erythritol, 1 g of L-malic acid.

[0084] Preparation method:

[0085] (1) Add 600 mL of water to 40 g of hawthorn, 3 g of phyllanthus emblica, chrysanthemum, 9 g of mulberry leaves, 4 g of poria cocos, 7 g of Chinese yam, 0.5 g of ginseng, 2.5 g of kudzu root, 0.5 g of mangosteen and decoct twice. After filtration, combine the filtrates;

[0086] (2) Add the formula amount of collagen peptide, sweetener, acidulant and donkey-hide gelatin to the combined filtrate, add water again and make up the volume to 1500 mL;

[0087] (3) Sterilize and fill the fixed-volume solution into cans and sub-pack, to obtain the oral liquid.

[0088] Example 9

[0089] An oral liquid with the efficacy of improving chloasma, and the components are as follows:

[0090] 50 g of hawthorn, 7.5 g of mulberry leaves, 2.5 g of phyllanthus emblica, 0.8 g of hangzhou white chrysanthemum, 1.6 g of kudzu root, 6 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.2 g of donkey-hide gelatin, 0.7 g of mangosteen, 80 g of collagen peptide, 51 g of erythritol, 1.2 g of L-malic acid.

[0091] The preparation method is the same as that of Example 8.

[0092] Example 10

[0093] An oral liquid with the efficacy of improving chloasma, and the components are as follows:

[0094] 35 g of hawthorn, 10 g of mulberry leaves, 2 g of phyllanthus emblica, 0.3 g of huangshan tribute chrysanthemum, 3 g of kudzu root, 9 g of Chinese yam, 2 g of poria cocos, 0.8 g of ginseng, 0.1 g of donkey-hide gelatin, 0.9 g of mangosteen, 90 g of collagen peptide, 60 g of erythritol, 0.5 g of L-malic acid.

[0095] The preparation method is the same as that of Example 8.

[0096] Example 11

[0097] An oral liquid with the efficacy of improving chloasma, and the components are as follows:

[0098] 25 g of hawthorn, 12 g of mulberry leaf, 1 g of phyllanthus emblica, 0.1 g of hangzhou white chrysanthemum, 5 g of kudzu root, 3 g of Chinese yam, 8 g of poria cocos, 0.1 g of ginseng, 0.9 g of donkey-hide gelatin, 0.1 g of mangosteen, 40 g of collagen peptide, 20 g of erythritol, 2 g of L-malic acid.

[0099] The preparation method is the same as that of Example 8.

[0100] Example 12

[0101] A powder with the efficacy of improving chloasma, the components are as follows:

[0102] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 45 g of erythritol, 1 g of L-malic acid.

[0103] Preparation method:

[0104] (1) Add 600 mL of water to decoct the hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen in the formula amount twice, filter and combine the filtrates, concentrate, dry, and pulverize to obtain the extract solid powder;

[0105] (2) Mix the solid powder of the extract with the collagen peptide, sweetener, acidulant, and donkey-hide gelatin in the formula amount evenly, and then pack and make into powder.

[0106] Example 13

[0107] A tablet with the efficacy of improving chloasma, the components are as follows:

[0108] 50 g of hawthorn, 7.5 g of mulberry leaf, 2.5 g of phyllanthus emblica, 0.8 g of hangzhou white chrysanthemum, 1.6 g of kudzu root, 6 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.2 g of donkey-hide gelatin, 0.7 g of mangosteen, 80 g of collagen peptide, 51 g of erythritol, 1.2 g of L-malic acid.

[0109] Preparation method:

[0110] (1) Add 600 mL of water to decoct the hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen in the formula amount twice, filter and combine the filtrates, and concentrate to obtain an extract;

[0111] (2) Add the collagen peptide, sweetener, acidulant, and donkey-hide gelatin in the formula amount to the extract, spray granulate and then press into tablets to obtain tablets.

[0112] Example 14

[0113] A capsule with the efficacy of improving chloasma, the components are as follows:

[0114] 35 g of hawthorn, 10 g of mulberry leaf, 2 g of phyllanthus emblica, 0.3 g of huangshi tribute chrysanthemum, 3 g of kudzu root, 9 g of Chinese yam, 2 g of poria cocos, 0.8 g of ginseng, 0.1 g of donkey-hide gelatin, 0.9 g of mangosteen, 90 g of collagen peptide, 60 g of erythritol, 0.5 g of L-malic acid.

[0115] Preparation method:

[0116] (1) Add 600 mL of water to decoct the hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen in the formula amounts twice, filter and combine the filtrates, and concentrate to obtain an extract.

[0117] (2) Add the collagen peptide, sweetener, acidulant, and donkey-hide gelatin in the formula amounts to the extract, spray granulate and fill into capsules to obtain capsule preparations.

[0118] Example 15

[0119] A granule with the efficacy of improving chloasma, the components are as follows:

[0120] 25 g of hawthorn, 12 g of mulberry leaf, 1 g of phyllanthus emblica, 0.1 g of hangzhou white chrysanthemum, 5 g of kudzu root, 3 g of Chinese yam, 8 g of poria cocos, 0.1 g of ginseng, 0.9 g of donkey-hide gelatin, 0.1 g of mangosteen, 40 g of collagen peptide, 20 g of erythritol, 2 g of L-malic acid.

[0121] Preparation method:

[0122] (1) Add 600 mL of water to decoct the hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen in the formula amounts twice, filter and combine the filtrates, and concentrate to obtain an extract.

[0123] (2) Add the collagen peptide, sweetener, acidulant, and donkey-hide gelatin in the formula amounts to the extract, spray granulate to obtain granule preparations.

[0124] Example 16

[0125] An oral liquid with the efficacy of improving chloasma, the components are as follows:

[0126] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 45 g of maltitol, 1 g of citric acid.

[0127] The preparation method is the same as that of Example 8.

[0128] Example 17

[0129] An oral liquid with the efficacy of improving chloasma, the components are as follows:

[0130] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 20 g of erythritol, 2 g of L-malic acid.

[0131] The preparation method is the same as that of Example 8.

[0132] Example 18

[0133] A powder with the efficacy of improving chloasma, the components are as follows:

[0134] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 70 g of xylitol, 0.1 g of ascorbic acid.

[0135] The preparation method is the same as that of Example 12.

[0136] Example 19

[0137] An oral liquid with the efficacy of improving chloasma, the components are as follows:

[0138] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 45 g of mannitol, 5 g of lactitol, 1 g of tartaric acid, 0.5 g of lactic acid.

[0139] The preparation method is the same as that of Example 8.

[0140] Comparative Example 1

[0141] AXXZIA ALESIA AG's fifth-generation anti-sugar oral liquid, product number: 100020357100.

[0142] Comparative Example 2

[0143] A composition with the efficacy of improving chloasma, the components are as follows:

[0144] 15 g of hawthorn, 15 g of mulberry leaf, 7 g of phyllanthus emblica, 1.5 g of hangzhou white chrysanthemum, 6 g of kudzu root, 14 g of Chinese yam, 9 g of poria cocos, 1.2 g of ginseng, 1 g of donkey-hide gelatin, 1.3 g of mangosteen, 110 g of collagen peptide.

[0145] The preparation method is the same as that of Example 1.

[0146] Comparative Example 3

[0147] A composition with the efficacy of improving chloasma, the components are as follows:

[0148] 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide.

[0149] Preparation method:

[0150] (1) Add 600 mL of water to decoct the poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen in the formula amount twice, filter, and combine the filtrates.

[0151] (2) Add the collagen peptide and donkey-hide gelatin in the formula amount to the combined filtrate, add water again and make up the volume to 1500 mL.

[0152] (3) Sterilize and fill the solution after volume adjustment into cans and then sub-pack.

[0153] Comparative Example 4

[0154] A composition with the efficacy of improving chloasma, the components are as follows:

[0155] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of Hangzhou white chrysanthemum, 0.5 g of mangosteen, 70 g of collagen peptide.

[0156] Preparation method:

[0157] (1) Add 600 mL of water to decoct the hawthorn, phyllanthus emblica, Hangzhou white chrysanthemum, mulberry leaf, and mangosteen in the formula amount twice, filter, and combine the filtrates.

[0158] (2) Add the collagen peptide in the formula amount to the combined filtrate, add water again and make up the volume to 1500 mL.

[0159] (3) Sterilize and fill the solution after volume adjustment into cans and then sub-pack.

[0160] Comparative Example 5

[0161] An oral liquid with the efficacy of improving chloasma, the components are as follows:

[0162] 15 g of hawthorn, 15 g of mulberry leaf, 7 g of phyllanthus emblica, 1.5 g of Hangzhou white chrysanthemum, 6 g of kudzu root, 14 g of Chinese yam, 9 g of poria cocos, 1.2 g of ginseng, 1 g of donkey-hide gelatin, 1.3 g of mangosteen, 110 g of collagen peptide, 75 g of erythritol, 4 g of L-malic acid.

[0163] The preparation method is the same as that of Example 8.

[0164] Comparative Example 6

[0165] An oral liquid with the efficacy of improving chloasma, the components are as follows:

[0166] 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 45 g of erythritol, 1 g of L-malic acid.

[0167] Preparation method:

[0168] (1) Decoct the formula amounts of poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen with 600 mL of water twice, filter, and combine the filtrates.

[0169] (2) Add the formula amounts of collagen peptide, sweetener, acidulant, and donkey-hide gelatin to the combined filtrate, add water again, and make up the volume to 1500 mL.

[0170] (3) Sterilize and sub-pack the solution after volume adjustment to obtain the oral liquid.

[0171] Comparative Example 7

[0172] An oral liquid with the efficacy of improving chloasma, the components are as follows:

[0173] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 0.5 g of mangosteen, 70 g of collagen peptide, 45 g of erythritol, 1 g of L-malic acid.

[0174] Preparation method:

[0175] (1) Decoct the formula amounts of hawthorn, phyllanthus emblica, hangzhou white chrysanthemum, mulberry leaf, and mangosteen with 600 mL of water twice, filter, and combine the filtrates.

[0176] (2) Add the formula amounts of collagen peptide, sweetener, and acidulant to the combined filtrate, add water again, and make up the volume to 1500 mL.

[0177] (3) Sterilize and sub-pack the solution after volume adjustment to obtain the oral liquid.

[0178] Comparative Example 8

[0179] An oral liquid with the efficacy of improving chloasma, the components are as follows:

[0180] 40 g of hawthorn, 9 g of mulberry leaf, 3 g of phyllanthus emblica, 0.5 g of hangzhou white chrysanthemum, 2.5 g of kudzu root, 7 g of Chinese yam, 4 g of poria cocos, 0.5 g of ginseng, 0.4 g of donkey-hide gelatin, 0.5 g of mangosteen, 70 g of collagen peptide, 17 g of erythritol, 3 g of L-malic acid.

[0181] The preparation method is the same as that of Example 8.

[0182] Experimental Example 1 Effect of the oral liquid of the present invention on the proliferation activity of B16 cells

[0183] 1 Materials

[0184] 1.1 Cells: B16 murine melanoma cells were derived from the New Drug Pharmacology Center of Lunan Pharmaceutical Group Co., Ltd.

[0185] 1.2 Drugs, Reagents

[0186] 1.2.1 Drugs

[0187] The oral liquids of Example 8, Example 9 and Comparative Example 1 of the present invention. Vitamin C was purchased from Sigma, USA.

[0188] 1.2.2 Reagents

[0189] RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco, USA; dimethyl sulfoxide (DMSO) and thiazolyl blue (MTT) were purchased from Sigma, USA.

[0190] 1.2.3 Dosages

[0191] The oral liquids of Example 8, Example 9 and Comparative Example 1 were diluted 10 times with PBS-sterilized distilled water. Vitamin C was prepared into 4 mg / mL with sterilized distilled water.

[0192] 2. Experimental Procedure

[0193] B16 cells were inoculated into RPMI-1640 medium containing 10% FBS and 1% double antibiotics (100 U / mL penicillin, 0.1 g / L streptomycin sulfate), and cultured in a 5% CO2 cell incubator at 37°C and saturated humidity for 24 h, then the medium was changed once. On the 3rd day after cell inoculation, the cells were digested with 0.25% trypsin for subculture. When the cells were subcultured to the 4th generation, cells in the logarithmic growth phase were taken for experiments.

[0194] The MTT method was used for detection. Cells in the logarithmic growth phase were digested with 0.25% trypsin, counted, and made into a single cell suspension of 4×10 4 cells / mL. 100 μL of the single cell suspension was inoculated into a 96-well cell culture plate. After inoculation for 24 h, the medium was changed to serum-free RPMI-1640 medium for standby. The experimental groups and the control group (Vc) were set, with 3 parallel replicates in each group. After intervention with the samples, the cells were cultured at 37°C, 5% CO2, and saturated humidity, and the action time groups of 24 h and 48 h were set respectively.

[0195] When the action time was up, the supernatant of the cells was carefully discarded, 5 g / L MTT solution was added to each well, and the cells were incubated in an incubator at 37°C and 5% CO2 for another 4 h. Then the supernatant was gently aspirated, 150 μL of DMSO was added, and the mixture was shaken for 10 min. The absorbance A was measured at 490 nm, and the inhibition rate was calculated.

[0196]

[0197] 3. Results

[0198] Skin melanocyte tissue can transfer melanin to the epidermal basal layer cells, thereby causing local skin melanin deposition and leading to the formation of spots. Therefore, the B16 cell proliferation rate is an important indicator for judging the spot-lightening effect. After calculation, the B16 mouse melanoma cell activity inhibition rate of Example 8 was 80.55%, the B16 mouse melanoma cell activity inhibition rate of Example 9 was 69.66%, and the inhibition rate of the commercially available whitening oral liquid in Comparative Example 1 was 52.20%. It can be seen that the oral preparation of the composition of the present invention has a high activity inhibition rate for B16 mouse melanoma cells, and is superior to commercially available products, and has good spot-lightening activity.

[0199] Experimental Example 2 Evaluation of the Lightening Effect of the Composition of the Present Invention

[0200] 1. Subjects

[0201] 270 volunteers aged 25-50 were selected, all of whom were healthy women with no trauma, skin disease, infectious disease, or long-term medication history. Their facial skin had different degrees of pregnancy spots, chloasma, sun spots, age spots, and other problems.

[0202] 2. Experimental process

[0203] The above trial subjects were divided into 9 groups according to the depth of pigmentation and age, with 30 people in each group. The first group tried the product of Example 1, the second group tried the product of Example 2, the third group tried the product of Example 3, the fourth group tried the product of Example 4, the fifth group tried the product of Example 6, the sixth group tried the product of Comparative Example 1, the seventh group tried the product of Comparative Example 2, the eighth group tried the product of Comparative Example 3, and the ninth group tried the product of Comparative Example 4. Each person took it once a day after meals, each time with a dosage of 30 mL, for 30 consecutive days, and no cosmetics with similar functions such as spot lightening were used during this period.

[0204] The subjective evaluation of the trial users after use was investigated by questionnaire survey, and the comparison of the photos of the bare face before and after using the product was carefully compared and statistically analyzed. Among them, significant means that both the subjective evaluation and the comparison photos can show obvious changes; effective means that the subjective evaluation is difficult to judge, but the comparison photos can show changes; ineffective means that there is no change in both. The statistical results are shown in Table 1.

[0205] 3. Results

[0206] Table 1 Evaluation table of the lightening effect of the subjects

[0207]

[0208] As can be seen from the results in the table, changes in the content and composition of the formula components in Examples 1-6 and Comparative Examples 1-4 will cause changes in the freckle-removing effect. Within the protection scope of the present invention, the freckle-removing effect of Example 1 is the most significant, and the effective rates of Examples 1 to 3 are relatively high. The chrysanthemum in Example 4 is selected from Huangshan Gongju, and the freckle-removing effect is slightly worse than that of the chrysanthemum selected from Hangbaiju in the formula of Example 3. When the formula amount is not appropriate (Comparative Example 2), or the formula is incomplete (Comparative Examples 3 and 4), the freckle-removing effect is significantly inferior to that of the products in the examples. In addition, the products of this invention patent are all superior to the product of Comparative Example 1, indicating that the composition of the present invention has a significant freckle-removing effect.

[0209] Experimental Example 3 Taste Test of Oral Preparations of the Composition of the Present Invention

[0210] Take the products obtained in Example 8, Example 9, Example 16, Example 17, Comparative Example 1 and Comparative Example 8 for taste test. The specific method is to select 90 volunteers and randomly divide them into 6 groups, with 15 people in each group. Let them taste the above six groups of products respectively, and evaluate and score the taste of the products. The total score of the evaluation is 5 points.

[0211] Among them, 5 = good taste, acceptable; 4 = relatively good taste, acceptable; 3 = average taste, relatively acceptable; 2 = relatively poor taste, barely acceptable; 1 = very poor taste, unacceptable. This grade includes the ability to use 0.1 points, so there is a possibility of 50 grade differences. The results are as follows in the table:

[0212] Results

[0213] Table 2 Taste Test Effect Table of Oral Preparations

[0214]

[0215] As can be seen from the above scoring results, the taste of this product is acceptable to the public. The taste of Example 8 is the best, and except for Comparative Example 8, the taste is mostly superior to that of Comparative Example 1.

Claims

1. A composition with the efficacy of improving chloasma, characterized in that, The active ingredients of the composition are made from the following raw materials in parts by weight: 25 - 60 parts of hawthorn, 4 - 12 parts of mulberry leaf, 1 - 6 parts of phyllanthus emblica, 0.1 - 1.2 parts of chrysanthemum, 0.5 - 5 parts of kudzu root, 3 - 12 parts of Chinese yam, 1 - 8 parts of poria cocos, 0.1 - 1 part of ginseng, 0.05 - 0.9 part of donkey-hide gelatin, 0.1 - 1 part of mangosteen, 40 - 100 parts of collagen peptide; the collagen peptide is deep-sea fish collagen peptide or freshwater fish collagen peptide; the chrysanthemum is selected from Hangzhou white chrysanthemum, Huangshan tribute chrysanthemum, Bozhou chrysanthemum, and Chuzhou chrysanthemum.

2. The composition according to claim 1, wherein The active ingredients of the composition are made from the following raw materials in parts by weight: 35 - 55 parts of hawthorn, 6 - 10 parts of mulberry leaf, 2 - 4 parts of phyllanthus emblica, 0.3 - 1 part of chrysanthemum, 1 - 3 parts of kudzu root, 4 - 9 parts of Chinese yam, 2 - 6 parts of poria cocos, 0.2 - 0.8 part of ginseng, 0.1 - 0.7 part of donkey-hide gelatin, 0.3 - 0.9 part of mangosteen, 50 - 90 parts of collagen peptide.

3. The composition according to claim 2, characterized in that, The active ingredients of the composition are made from the following raw materials in parts by weight: 40 parts of hawthorn, 9 parts of mulberry leaf, 3 parts of phyllanthus emblica, 0.5 part of chrysanthemum, 2.5 parts of kudzu root, 7 parts of Chinese yam, 4 parts of poria cocos, 0.5 part of ginseng, 0.4 part of donkey-hide gelatin, 0.5 part of mangosteen, 70 parts of collagen peptide.

4. The composition according to claim 1, characterized in that, The composition is prepared by the following steps: (1) Add 400 - 800 mL of water to decoct hawthorn, phyllanthus emblica, chrysanthemum, mulberry leaf, poria cocos, Chinese yam, ginseng, kudzu root, and mangosteen in the formula amounts twice, filter, and combine the filtrates; (2) Add the collagen peptide and donkey-hide gelatin in the formula amounts to the combined filtrate, add water again and make up the volume to 1300 - 1700 mL; (3) Sterilize and fill the made-up solution into cans and sub-pack.

5. An oral preparation, characterized in that, The oral preparation contains the composition as described in any one of claims 1 - 4.

6. The oral preparation according to claim 5, wherein The oral preparation further contains food-acceptable additives.

7. The oral preparation according to claim 6, characterized in that, The food-acceptable additives contain 0.1 - 2 parts of acidulant and 20 - 70 parts of sweetener; the acidulant is L-malic acid; the sweetener is erythritol.

8. The oral preparation according to claim 7, characterized in that, The food-acceptable additives contain 0.5 - 1.5 parts of acidulant and 30 - 60 parts of sweetener.

9. The oral preparation according to claim 8, characterized in that, The food-acceptable additives contain 1 part of acidulant and 45 parts of sweetener.

10. The oral preparation according to claim 5, characterized in that, The oral preparation is powder, granule, oral liquid, capsule, or tablet.

11. Use of a composition as described in any one of claims 1 - 4 or an oral preparation as described in any one of claims 5 - 10 in the preparation of a health food with the efficacy of improving chloasma.

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