A reactive fluorescent probe for mercury ions and / or silver ions, its preparation method and application

Abstract

This invention relates to the field of chemical analysis and detection technology, specifically to a reactive fluorescent probe for mercury ions and / or silver ions, its preparation method, and its application. The fluorescent probe has the structure shown in formula (I). The fluorescent probe of this invention can simultaneously and selectively detect mercury ions and silver ions in a dual-channel manner. It can detect mercury ions in solution, aggregated state, and solid state, and exhibits excellent selectivity and anti-interference ability. After reacting with mercury ions and adding water to form an aggregated state, the fluorescence enhancement increases by 486 times, accompanied by a change in fluorescence color from dark to bright yellow-green.

Description

Technical Field

[0001] This invention relates to the field of chemical analysis and detection technology, specifically to a reactive mercury ion and / or silver ion fluorescent probe, its preparation method, and its application. Background Technology

[0002] Mercury is one of the most toxic heavy metal pollutants, posing a significant threat to ecosystems regardless of its form. More seriously, inorganic mercury ions can accumulate along the food chain and transform into organic mercury, significantly increasing their harm to human health. Similar to mercury ions, while silver ions have lower toxicity, excessive amounts of Ag in organisms can lead to adverse effects. + It can bind to thiol enzymes, causing enzyme inactivation and leading to a series of diseases. Compared with traditional detection methods such as mass spectrometry and atomic absorption spectrometry, fluorescence technology has been widely used due to its advantages of simple operation, low cost, good selectivity, and high sensitivity.

[0003] Sensors designed based on specific chemical reactions between probe molecules and metal ions have attracted considerable attention due to their excellent selectivity. (Based on Hg...) 2+ and Ag + Due to the high affinity of Hg for sulfur, many sulfur-based acceptor probes have been developed. However, due to Hg... 2+ and Ag + Reactivity comparable to that of a probe often yields the same reaction products and signal output. Therefore, Ag + or Hg 2+ The presence of [a specific metal ion] often interferes with the detection of other metal ions, and the selective identification of mercury and silver ions is a significant challenge. A cleverly designed, ingenious probe is needed to achieve [the detection of] Hg [a specific metal ion]. 2+ and Ag + The differentiation of thiophilic activity results in different reaction products and output signals, thus completing the determination of Hg. 2+ and Ag + Meanwhile, selective detection will have significant theoretical and practical application value. Summary of the Invention

[0004] One objective of this invention is to provide a reactive fluorescent probe for mercury ions and / or silver ions, which can simultaneously and selectively detect Hg in solution. 2+ (red fluorescent signal) and Ag + (Green fluorescent signal) enables highly selective and sensitive detection of mercury ions in both aggregated and solid states.

[0005] The second objective of this invention is to provide a method for preparing reactive mercury ion and / or silver ion fluorescent probes. The preparation method is simple, easy to operate, uses inexpensive and readily available raw materials, and is convenient for industrial production.

[0006] A third objective of this invention is to provide a method for detecting mercury ions and silver ions in solution using a reactive fluorescent probe for mercury ions and / or silver ions.

[0007] The fourth objective of this invention is to provide a method for detecting mercury ions in the aggregated state using reactive fluorescent probes of mercury ions and / or silver ions.

[0008] The fifth objective of this invention is to provide a mercury ion detection test strip.

[0009] The sixth objective of this invention is to provide a method for detecting mercury ions using a mercury ion test strip.

[0010] One of the solutions adopted to achieve the objective of this invention is: a reactive mercury ion and / or silver ion fluorescent probe having the structure shown in formula (I):

[0011]

[0012] The second objective of this invention is achieved through a method for preparing the aforementioned reactive mercury ion and / or silver ion fluorescent probe, comprising the following steps:

[0013] (1) A certain amount of triphenylamine and anhydrous aluminum trichloride powder were added to organic solvent I, and then a certain amount of terephthaloyl chloride was added. The reaction was carried out completely at a certain temperature, and the compound shown in formula (II) was obtained after purification.

[0014] (2) The compound shown in formula (II) was added to organic solvent II and mixed well. 1,2-ethanedithiol and boron trifluoride-diethyl ether complex were added dropwise under stirring. The reaction was completed at a certain temperature and the compound shown in formula (I) was obtained after purification.

[0015] Preferably, in step (1), the molar ratio of terephthaloyl chloride, triphenylamine, and aluminum trichloride is 1:2-3:2-3; the reaction temperature is 50-70°C; and the organic solvent I is any one of dichloromethane, trichloromethane, and tetrahydrofuran.

[0016] Preferably, in step (1), the purification method is as follows: after the reaction is completed, the reaction solution is extracted with dichloromethane, the organic phases are combined and evaporated to dryness to obtain the crude product; then, the crude product is separated by silica gel column chromatography using a mixed solvent of petroleum ether and dichloromethane in a volume ratio of 1:6 as the eluent.

[0017] Preferably, in step (2), the molar ratio of the compound represented by formula (II), 1,2-ethanedithiol, and boron trifluoride-diethyl ether complex is 1:3 to 4:6 to 8, the reaction temperature is room temperature, and the organic solvent II is any one of dichloromethane, trichloromethane, and tetrahydrofuran.

[0018] Preferably, in step (2), the purification method is as follows: after the reaction is completed, the pH value of the reaction solution is adjusted to 8-9, and dichloromethane is used for extraction. After the organic phases are combined, the solution is evaporated to dryness to obtain the crude product. Then, the crude product is separated by silica gel column chromatography using a mixed solvent of petroleum ether and dichloromethane in a volume ratio of 1:1 as the eluent.

[0019] The pH of the reaction solution is adjusted using NaHCO3 or other weak bases.

[0020] The solution adopted to achieve the third objective of this invention is: a method for detecting mercury ions and silver ions in solution using the fluorescent probe described above, comprising the following steps: dissolving the fluorescent probe described in claim 1 in a good solvent to prepare a solution, then adding different metal ions and mixing evenly; under ultraviolet light irradiation, if the solution fluorescence color is red, the presence of mercury ions is detected; if the solution fluorescence color is green, the presence of silver ions is detected.

[0021] The solution adopted to achieve the fourth objective of this invention is: a method for detecting mercury ions in the aggregated state using the fluorescent probe described above, comprising the following steps: dissolving the fluorescent probe described in claim 1 in a good solvent to prepare a solution, then adding mercury ions, mixing evenly, and diluting with a poor solvent to obtain a dispersion; under ultraviolet light irradiation, if the fluorescence color of the dispersion is yellow-green, then the presence of mercury ions is detected; if the dispersion has almost no fluorescence, then the presence of mercury ions is not detected.

[0022] The fifth objective of this invention is achieved through a solution comprising: a mercury ion detection test strip, prepared by a method including the following steps: preparing the mercury ion fluorescent probe to a concentration of 1×10⁻⁶. -3 ~1×10 -2 The paper strip is immersed in a mol / L good solvent solution, then removed and air-dried to obtain the mercury ion detection test strip.

[0023] The solution adopted to achieve the sixth objective of this invention is: a method for detecting mercury ions using the aforementioned mercury ion detection test strip, comprising the following steps: wetting the mercury ion detection test strip in a solution containing mercury ions, removing it, drying it, and under ultraviolet light irradiation, the fluorescence of the test strip changes from dark to yellow-green, thus detecting the presence of mercury ions.

[0024] The good solvent for the fluorescent probe is an organic solvent that is miscible with the bad solvent. Preferably, the good solvent is tetrahydrofuran, and the bad solvent is water, n-hexane, ethanol, etc. Preferably, the bad solvent is water.

[0025] The fluorescent probe can only detect mercury and silver ions in a good solvent (the volume of poor solvent in the solution should not exceed 10%). The probe molecules do not fluoresce in solution or in the solid state. In a good solvent solution, they react with mercury and silver ions to produce red and green fluorescence, respectively, thus allowing for selective detection of Hg. 2+ and Ag + When the volume percentage of unsuitable solvent in the solution is ≥90%, the fluorescent probe forms nanoparticles and aggregates. In this state, the product of the reaction between the fluorescent probe and mercury ions exhibits strong yellow-green fluorescence, while the product obtained from the reaction with silver ions shows no fluorescence under these conditions. When the fluorescent probe is in a solid state (e.g., test strips), the product of the reaction between the fluorescent probe and mercury ions also exhibits strong yellow-green fluorescence, while the product obtained from the reaction with silver ions shows no fluorescence under these conditions. Therefore, the fluorescent probe can only detect mercury ions in its aggregated or solid state.

[0026] This invention designs and synthesizes a fluorescent probe TAS containing two thioclase groups, achieving the detection of Hg in solution. 2+ (red fluorescence) and Ag + The dual-channel selective "light-up" detection of (green fluorescence) had a detection limit of 9.2 × 10⁻⁶. -8 mol / L and 8.6×10 -7 mol / L. The probe TAS can cleverly distinguish Hg. 2+ and Ag + Different thiophilic reactivity of ions leads to different reaction products and signal outputs. Specifically, Hg 2+ The ions can immediately convert the two thioketal groups in TAS into carbonyl groups, yielding the reaction product TAO. However, under the same conditions, Ag... + The ion can only convert one thioacetate in TAS to a carbonyl group, yielding a partially deprotected product. Furthermore, due to the significant aggregation-induced emission properties of TAO, Hg... 2+ The triggered TAS to TAO transition allows the probe to target Hg in both aggregated and solid states. 2+ It also exhibits excellent sensing performance. The probes TAS and Hg... 2+ After the reaction, water was added to form an aggregated state (containing 98% water), which increased the fluorescence intensity by 486 times. In the solid state, the prepared test strip can detect Hg in water with high sensitivity. 2+ Under the illumination of a handheld ultraviolet lamp, without the need for other complex instruments, the fluorescence of the test strip changes from dark to bright yellow-green directly with the naked eye, and the detection limit is 1×10⁻⁶. -6 mol / L.

[0027] The present invention has the following advantages and beneficial effects:

[0028] (1) The reactive mercury ion and / or silver ion fluorescent probe of the present invention can simultaneously and selectively detect mercury ions and silver ions through dual channels, so as to achieve Hg 2+ (red fluorescence) and Ag + (Green fluorescence) dual-channel selective "on" detection.

[0029] (2) The reactive mercury ion and / or silver ion fluorescent probes of the present invention can detect mercury ions in solution, aggregated state and solid state, and have excellent selectivity and anti-interference ability.

[0030] (3) The reactive mercury ion and / or silver ion fluorescent probe of the present invention reacts with mercury ions and then adds water to form an aggregated state, which increases the fluorescence enhancement by 486 times, accompanied by the fluorescence color changing from dark to bright yellow-green.

[0031] (4) The preparation method of the present invention uses inexpensive and readily available triphenylamine as raw material and synthesizes a probe molecule containing two thioclase units through two simple reactions, which is a reactive mercury ion and / or silver ion fluorescent probe. The preparation method is simple, the raw materials are inexpensive and readily available, and it is convenient for industrial production.

[0032] (5) The test strip prepared by this invention has high sensitivity to aqueous solutions of mercury ions. Under ultraviolet light irradiation, the detection limit for mercury ions by naked-eye observation alone is 1×10⁻⁶. -6 mol / L. Attached Figure Description

[0033] Figure 1 The molecular structure and sensing process of compound TAS are shown.

[0034] Figure 2 The fluorescence response diagrams and fluorescence photographs of the tetrahydrofuran solution of compound TAS to different metal ions are shown.

[0035] Figure 3 After adding different metal ions to the compound TAS, water was added to obtain the fluorescence spectra of the aggregated state.

[0036] Figure 4 After adding different metal ions to the compound TAS, water was added to obtain fluorescence response patterns and fluorescence photographs of the aggregated state.

[0037] Figure 5 These are photographs of the fluorescence response of the test strip to different concentrations of mercury ions. Detailed Implementation

[0038] To better understand the present invention, the following embodiments are further illustrations of the present invention, but the content of the present invention is not limited to the following embodiments.

[0039] The raw materials used in the embodiments of the present invention can be purchased from the market or synthesized using methods known in the art.

[0040] A Hg based on deprotection reaction 2+ and / or Ag + The ion fluorescent probe (hereinafter referred to as compound TAS) has the structure shown in formula (I):

[0041] A method for preparing the above-mentioned mercury ion fluorescent probe includes the following steps:

[0042] (1) At room temperature, triphenylamine and anhydrous aluminum trichloride powder were added to dry dichloromethane, followed by the addition of terephthaloyl chloride, wherein the molar ratio of terephthaloyl chloride, triphenylamine, and aluminum trichloride was 1:2-3:2-3; the reaction was carried out at 50-70°C until complete, and the compound represented by formula (II) (hereinafter referred to as compound TAO) was obtained after purification.

[0043] (2) Under a nitrogen atmosphere (or an air atmosphere or other inert atmosphere), the compound shown in formula (II) and the boron trifluoride-diethyl ether complex are dissolved in anhydrous dichloromethane, and 1,2-ethanedithiol is added dropwise under stirring. The molar ratio of the compound shown in formula (II), 1,2-ethanedithiol and the boron trifluoride-diethyl ether complex is 1:3 to 4:6 to 8. The reaction is carried out at room temperature until the desired temperature is reached. The mixture is then purified to obtain the compound shown in formula (I).

[0044] The purification method described in step (1) is as follows: after the reaction is completed, the reaction solution is extracted with dichloromethane, the organic phases are combined and evaporated to dryness to obtain the crude product; then, the crude product is separated by silica gel column chromatography using a mixed solvent of dichloromethane and petroleum ether in a volume ratio of 6:1 as the eluent.

[0045] The purification method described in step (2) is as follows: After the reaction is completed, the pH value of the reaction solution is adjusted to 8-9, and dichloromethane is used for extraction. After the organic phases are combined, the solution is evaporated to dryness to obtain the crude product. Then, a mixed solvent of dichloromethane and petroleum ether in a volume ratio of 1:1 is used as the eluent to separate the crude product by silica gel column chromatography.

[0046] Example 1

[0047] The synthetic route for compound TAS is as follows:

[0048] (1) Compound TAO was first synthesized via Friedel-Crafts acylation. The specific synthetic steps are as follows: At room temperature, 40 mL of dry dichloromethane was added to a reaction flask, followed by triphenylamine (TPA) (2.45 g, 10 mmol) and thoroughly ground AlCl3 (1.33 g, 10 mmol). Terephthaloyl chloride (1.02 g, 5 mmol) was added with stirring, and the reaction was carried out at 60 °C for 24 hours. After the reaction was complete, the reaction solution was extracted with dichloromethane, and the combined organic phases were evaporated to dryness to obtain the crude product. The crude product was separated by silica gel column chromatography using a 1:6 volume ratio of petroleum ether to dichloromethane as the eluent to obtain a yellow solid product with a yield of 62%. The NMR data are as follows: 1 H NMR (300MHz, CDCl3): 7.82 (s, 4H, ArH), 7.73 (d, J = 9.0, 4H, ArH), 7.36 (m, 8H, ArH), 7.20 (m, 12H, ArH), 7.02 (d, J = 6.0, 4H, ArH). 13 C NMR (75MHz, CDCl3): 194.5, 152.3, 146.3, 141.1, 132.1, 129.7, 129.3, 128.9, 126.2, 124.9, 119.3.

[0049] (2) Synthesis of compound TAS. The specific synthetic steps are as follows: TAO (620 mg, 1 mmol) and 1,2-ethanedithiol (0.34 mL, 4 mmol) were dissolved together in dry dichloromethane, and then BF3·Et2O (1 mL, 8 mmol) was added. The reaction was carried out at room temperature for 24 hours. After the reaction was completed, NaHCO3 aqueous solution was added to adjust the pH of the reaction solution to 8-9, and the solution was extracted with dichloromethane. The organic phases were combined and evaporated to dryness to obtain the crude product. Then, the crude product was separated by silica gel column chromatography using a 1:1 volume ratio of petroleum ether and dichloromethane as the eluent to obtain a light yellow solid product with a yield of 36%. The NMR data are as follows: 1 HNMR(300MHz,THF-d8):7.54(s,4H,ArH),7.44(d,J=9.0,4H,ArH),7.22(t,J=6.0,8H,ArH),7.06(d,J=6.0 ,8H,ArH),7.00(t,J=6.0,4H,ArH),6.91(d,J=6.0,4H,ArH),7.02(d,J=6.0,4H,ArH),3.58(m,8H,-SCH2-). 13C NMR (75MHz, THF-d8): 147.7, 146.8, 143.7, 137.8, 129.3, 129.1, 127.8, 124.4, 122.9, 121.9, 76.4, 39.9.

[0050] Example 2

[0051] Fluorescence detection of mercury and silver ions in solution by compound TAS

[0052] Prepare concentrations of 1×10 -1 Aqueous solutions of Hg(ClO4)2·3H2O, AgNO3, Al(NO3)3·9H2O, Cr(NO3)3·9H2O, Fe(NO3)3·9H2O, Co(NO3)2·6H2O, Ba(NO3)2, Ca(NO3)2·4H2O, Pb(NO3)2, Ni(NO3)2·6H2O, Zn(NO3)2·6H2O, Mn(NO3)2·4H2O, Cu(NO3)2·3H2O, Cd(NO3)2·4H2O, Mg(ClO4)2, Fe(ClO4)2, NaNO3, KNO3, and LiNO3 in mol / L concentrations were diluted to the required concentrations before testing.

[0053] Prepare a THF (tetrahydrofuran) solution of compound TAS prepared in Example 1 with a concentration of 2 × 10⁻⁶. -5 mol / L, at which point the solution shows almost no fluorescence. Various metal cation solutions (1×10⁻⁶) were prepared. -1 Adding 18 μL of mol / L metal ions to a 3 mL THAF solution yields a final metal ion concentration of 6 × 10⁻⁶. -4 M. The changes in fluorescence intensity of the reaction solution caused by the addition of different metal ions were recorded using a fluorescence spectrometer. For example... Figure 1 and Figure 2 As shown, mercury ions can induce a complete deprotection reaction of TAS to yield the corresponding carbonyl compound TAO, with the fluorescence color changing from dark to red. Simultaneously, silver ions can induce a partial deprotection reaction of TAS, with the fluorescence color changing from dark to bright green. All other ions do not react with TAS, resulting in a near-non-fluorescent state. This significant difference in fluorescence can be directly distinguished by the naked eye under ultraviolet light. This demonstrates that the compound TAS of the present invention can achieve the reaction of Hg... 2+ and Ag + It features dual-channel selective detection with excellent selectivity.

[0054] Example 3

[0055] Fluorescence detection of mercury ions in the aggregated state by compound TAS

[0056] Prepare a THF (tetrahydrofuran) solution of compound TAS prepared in Example 1 with a concentration of 1×10⁻⁶. -3 mol / L. Various metal cation solutions (1×10⁻⁶) were prepared. -1 18 μL of TAS was added to a 60 μL THF solution, and then deionized water was added to dilute the solution to a total volume of 3 mL (where the volume fraction of water was 98%). Since TAS and the products obtained from its reaction with metal ions are sparingly soluble in water, the addition of water will gradually form an aggregated state. The changes in fluorescence intensity of the reaction solution caused by the addition of different metal ions were recorded using a fluorescence spectrometer.

[0057] Different metal ions Hg 2+ Ag + Fe 3+ Cu 2+ Pb 2+ Co 2+ Cr 3+ Al 3+ Cd 2+ Mg 2+ Mn 2+ Ba 2+ Fe 2+ Ca 2+ Ni 2+ Zn 2+ Li + K + and Na + When added to a THAS THF solution, followed by the addition of deionized water, an aggregated state is formed, resulting in a final metal ion concentration of 6 × 10⁻⁶. -4 The final concentration of M, TAS is 2 × 10 -5 M. Due to its significant aggregation-induced emission properties, TAO exhibits a bright yellow-green emission in its aggregated state with a water content of 98%. For example... Figure 3 and Figure 4 As shown, only the addition of mercury ions can induce the TAS deprotection reaction to obtain TAO, and the corresponding fluorescence color changes from dark to yellow-green, with the fluorescence intensity increasing by 486 times.

[0058] Example 4

[0059] Compound TAS was used as a test strip to detect the fluorescence response of mercury ions.

[0060] Compared to solution, test strips are a more convenient and effective detection method in practical applications, and they better reflect the true sensitivity (the detection limit in solution is generally the concentration after significant dilution of the mother liquor). We soaked the test strips in a TAS tetrahydrofuran solution (1×10⁻⁶). -3The test strip is prepared at a concentration of mol / L, and then removed and air-dried. Just like pH test strips, the prepared test strip is used to detect different concentrations of Hg. 2+ In aqueous solution. For example... Figure 5 As shown, due to the excellent aggregation-induced emission properties of the product TAO obtained from the reaction with mercury ions, the test strip exhibits a significant fluorescence response to mercury ions under ultraviolet light irradiation, with a detection limit of 1.0 × 10⁻⁶. -6 M. To further verify its practical application value, we prepared mercury ion aqueous solutions of different concentrations directly using tap water and river water. The detection limit of the test strip could still reach 1.0 × 10⁻⁶. -6 M. Therefore, this test strip can be used to conveniently detect whether the mercury ion concentration in a sample exceeds the standard.

[0061] The probe provided by this invention can selectively detect mercury ions and silver ions in THF solution through dual channels. It can also effectively detect mercury ions in both aggregated and solid states, exhibiting excellent selectivity and sensitivity. Combined with the ease of preparation and use of the test strip, it has practical application potential.

[0062] The above description is merely a preferred embodiment of the present invention, and should not be construed as limiting the scope of the invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A reactive fluorescent probe for mercury ions and / or silver ions, characterized in that, It has the structure shown in equation (I):

2. A method for preparing a reactive mercury ion and / or silver ion fluorescent probe as described in claim 1, characterized in that, Includes the following steps: (1) A certain amount of triphenylamine and anhydrous aluminum trichloride powder were added to organic solvent I, and then a certain amount of terephthaloyl chloride was added. The reaction was carried out completely at a certain temperature, and the compound shown in formula (II) was obtained after purification. (2) The compound shown in formula (II) was added to organic solvent II and mixed well. 1,2-ethanedithiol and boron trifluoride-diethyl ether complex were added dropwise under stirring. The reaction was completed at a certain temperature and the compound shown in formula (I) was obtained after purification.

3. The preparation method according to claim 2, characterized in that: In step (1), the molar ratio of terephthaloyl chloride, triphenylamine and aluminum trichloride is 1:2-3:2-3; the reaction temperature is 50-70℃; and the organic solvent I is any one of dichloromethane, trichloromethane and tetrahydrofuran.

4. The preparation method according to claim 2, characterized in that: In step (1), the purification method is as follows: after the reaction is completed, the reaction solution is extracted with dichloromethane, the organic phases are combined and evaporated to dryness to obtain the crude product; then, the crude product is separated by silica gel column chromatography using a mixed solvent of petroleum ether and dichloromethane in a volume ratio of 1:6 as the eluent.

5. The preparation method according to claim 2, characterized in that: In step (2), the molar ratio of the compound represented by formula (II), 1,2-ethanedithiol, and boron trifluoride-diethyl ether complex is 1:3-4:6-8, the reaction temperature is room temperature, and organic solvent II is any one of dichloromethane, trichloromethane, and tetrahydrofuran.

6. The preparation method according to claim 2, characterized in that: In step (2), the purification method is as follows: after the reaction is completed, the pH value of the reaction solution is adjusted to 8-9, and dichloromethane is used for extraction. After the organic phases are combined, the solution is evaporated to dryness to obtain the crude product. Then, the crude product is separated by silica gel column chromatography using a mixed solvent of petroleum ether and dichloromethane in a volume ratio of 1:1 as the eluent.

7. A method for detecting mercury ions and silver ions in solution using the fluorescent probe according to claim 1, characterized in that, The method includes the following steps: dissolving the fluorescent probe of claim 1 in a good solvent to prepare a solution, then adding different metal ions and mixing evenly. Under ultraviolet light irradiation, if the solution fluorescence color is red, the presence of mercury ions is detected; if the solution fluorescence color is green, the presence of silver ions is detected.

8. A method for detecting mercury ions in an aggregated state using the fluorescent probe of claim 1, characterized in that, The method includes the following steps: dissolving the fluorescent probe of claim 1 in a good solvent to prepare a solution, then adding mercury ions, mixing evenly, and diluting with a poor solvent to obtain a dispersion. Under ultraviolet light irradiation, if the fluorescence color of the dispersion is yellow-green, the presence of mercury ions is detected; if the dispersion has almost no fluorescence, the presence of mercury ions is not detected.

9. A mercury ion detection test strip, characterized in that, The mercury ion fluorescent probe of claim 1 is prepared by a method comprising the following steps: The mercury ion fluorescent probe of claim 1 is formulated to a concentration of 1×10⁻⁶. -3 ~1×10 -2 The paper strip is immersed in a mol / L good solvent solution, then removed and air-dried to obtain the mercury ion detection test strip.

10. A method for detecting mercury ions using the mercury ion detection test strip according to claim 9, characterized in that, The procedure includes the following steps: wetting the mercury ion detection test strip as described in claim 9 in a solution containing mercury ions, removing it, drying it, and under ultraviolet light irradiation, the fluorescence of the test strip changes from dark to yellow-green, indicating the detection of the presence of mercury ions.

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