A liquid microbial inoculant application method that can be adapted for different crops

Abstract

The present application belongs to the technical field of microbial inoculant, and particularly relates to a liquid microbial inoculant application method applicable to different crops, comprising the following steps: S1, dissolving the microbial inoculant in 50-90wt% water to obtain a liquid microbial inoculant; S2, selecting a target crop, determining the fertilization requirement according to the growth stage, and uniformly applying the liquid microbial inoculant to the soil at the root of the target crop; S3, performing field management, regularly irrigating, controlling soil humidity, regularly weeding, and timely preventing and treating diseases and pests. The active microbial strains in the liquid microbial inoculant can form a symbiotic relationship with the roots of crops, promote the absorption and utilization of nutrients by crops, and thus increase the yield of crops. Meanwhile, the beneficial microorganisms in the microbial inoculant can decompose harmful substances in the soil, improve the soil environment, and improve the quality of crops. The microbial inoculant does not contain chemical synthetic pesticides and fertilizer components, is friendly to the environment and ecology, meets the requirements of green and sustainable development.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of microbial inoculant, and particularly relates to a liquid microbial inoculant application method applicable to different crops. BACKGROUND

[0002] Microbial inoculant is a symbiotic bacteria formed by artificially modifying microbial strains isolated from nature. It has been widely used in environmental protection and agriculture. Microbial inoculant plays a key role in water, gas and solid waste treatment, accelerates treatment efficiency, maintains system stability and improves the removal effect of specific pollutants. Compared with other treatment methods, it has the advantages of no secondary pollution, on-site treatment and simple operation. Liquid microbial inoculant is a kind of microbial inoculant, which is simple to prepare, easy to add and fast to take effect, but it lacks comprehensive research on various factors in preparation, has high application cost, and may cause changes in microbial population and ecological safety problems in actual environment. However, liquid microbial inoculant still has wide application prospects in many fields, especially in the field of agriculture. Liquid microbial inoculant can significantly increase the number and activity of beneficial microorganisms in soil, form a symbiotic relationship with crop roots, improve nutrient uptake efficiency, and promote the healthy growth of crops. In addition, liquid microbial inoculant can also improve soil structure and improve soil water and fertilizer retention capacity.

[0003] In the prior art, the survival rate of beneficial microorganisms in traditional liquid microbial inoculant is low, which is easy to be inactivated during storage, transportation and application, affecting the effect. Its fluidity leads to uneven application, and is greatly affected by environmental conditions such as temperature, humidity and pH value, making it difficult to maintain an ideal survival environment, thereby affecting reproduction and application effect. SUMMARY

[0004] The present application aims to overcome the shortcomings of the prior art and provide a liquid microbial inoculant application method applicable to different crops.

[0005] The purpose of the present application can be achieved by the following technical solutions:

[0006] A liquid microbial inoculant application method applicable to different crops, comprising the following steps:

[0007] S1, dissolving microbial inoculant in 50-90wt% water to obtain liquid microbial inoculant;

[0008] S2, selecting target crops, determining fertilizer demand according to their growth stages, and uniformly applying liquid microbial inoculant to the root soil of target crops;

[0009] S3, field management, regular irrigation, control soil humidity at 60-80%, regular weeding, and timely prevention and control of diseases and pests.

[0010] Further, the microbial inoculant in step S1 includes the following raw materials by weight: 5-7 parts of active microbial strains, 10-20 parts of modified carriers, 30-40 parts of water-soluble organic fertilizers, 1-3 parts of protective agents, and 2-4 parts of ethylenediaminetetraacetic acid.

[0011] Further, the fertilization requirement in step S2 is specifically:

[0012] A1. Within 7-14 days after the target crop germination, the fertilization frequency is once every 2-3 days, and each time 5-10 g / m of liquid microbial inoculant is applied. 2 ;

[0013] A2. The fertilization frequency during the seedling growth period is once every 1-2 days, and each time 10-20 g / m of liquid microbial inoculant is applied. 2 ;

[0014] A3. From before flowering to the end of the flowering period, the fertilization frequency is once every 7 days, and each time 30-50 g / m of liquid microbial inoculant is applied. 2 ;

[0015] A3. During the fruiting period, the fertilization frequency is once every 5-7 days, and each time 20-40 g / m of liquid microbial inoculant is applied. 2 .

[0016] The protective agent includes one or more of trehalose, polyethylene glycol, carboxymethyl cellulose, glycine, and proline.

[0017] Further, the modified carrier is prepared by the following steps:

[0018] Calcium chloride and disodium hydrogen phosphate are added to 50-60 wt% ammonia water, mixed uniformly, stirred for 1-2 h, centrifuged, washed, and hydroxyapatite is obtained. The hydroxyapatite is dispersed in anhydrous ethanol, and phosphatidylserine is added. Stirring is performed for 2-4 h, and then polyethyleneimine is continuously added, with the temperature controlled at 50-60°C. The reaction is continued for 4-5 h, and then centrifugation, washing, and drying are performed to obtain polyethyleneimine-loaded phosphatidylserine-modified hydroxyapatite, i.e., the modified carrier.

[0019] The active microbial strain suspension is inoculated onto the modified carrier after 3-5 days of culture, and the active microbial strain includes one or more of Bacillus subtilis, lactic acid bacteria, actinomycetes, Bacillus megaterium, and rhizobium.

[0020] The water-soluble organic fertilizer includes the following raw materials by weight: 10-20 parts of humic acid, 40-60 parts of organic matter, 10-14 parts of urea, 7-9 parts of potassium dihydrogen phosphate, and 5-7 parts of potassium nitrate.

[0021] The mass ratio of the calcium chloride, the disodium hydrogen phosphate, the phosphatidylserine and the polyethyleneimine is (10-15):(8-14):(5-7):(20-22).

[0022] Advantages of the present application:

[0023] 1、In the technical scheme of the present application, the hydroxyapatite has a skeleton structure formed by the connection of calcium ions and phosphate ions, wherein the hydroxyl groups are connected with the phosphate ions to form a crystal network. The phosphate groups of the phosphatidylserine can form stable coordination bonds with the calcium ions in the hydroxyapatite, thereby enhancing the interaction with the surrounding environment, improving the dispersion and stability in the liquid microbial inoculant. The amino groups of the polyethyleneimine can form hydrogen bonds or ionic bonds with the hydroxyl groups or other functional groups on the surface of the hydroxyapatite, thereby improving the mechanical strength, stability and adsorption and dispersion performance of the modified carrier.

[0024] 2、In the technical scheme of the present application, the modified carrier can provide sites for the attachment of beneficial microorganisms, thereby increasing the stability of the inoculant during storage, transportation and application, and reducing the inactivation rate.

[0025] 3、In the technical scheme of the present application, the modified carrier can adjust the affinity of the carrier to the soil around the crop roots by introducing phosphatidylserine, so that it can better adapt to changes in the environment of different soil types, thereby maintaining a high application effect.

[0026] 4、In the technical scheme of the present application, the active microbial strains in the liquid microbial inoculant can form a symbiotic relationship with the crop roots, promoting the absorption and utilization of nutrients by the crops, thereby increasing the yield of the crops. At the same time, the beneficial microorganisms in the microbial inoculant can decompose harmful substances in the soil, improve the soil environment and improve the quality of the crops. Moreover, it does not contain chemical synthetic pesticides and fertilizers, is friendly to the environment and ecology, and meets the requirements of green and sustainable development. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0028] Example 1

[0029] The modified carrier is prepared by the following steps:

[0030] 10 g of calcium chloride and 8 g of disodium hydrogen phosphate were added to 50 wt% ammonia water, mixed uniformly, stirred for 1 h, centrifuged, washed, and hydroxyapatite was obtained. The hydroxyapatite was dispersed in anhydrous ethanol, 5 g of phosphatidylserine was added, stirred for 2 h, 20 g of polyethyleneimine was continuously added, the temperature was controlled at 50°C, and the reaction was continued for 4 h. After centrifugation, washing, and drying, polyethyleneimine-loaded phosphatidylserine-modified hydroxyapatite, i.e., a modified carrier, was prepared.

[0031] Example 2

[0032] The modified carrier was prepared by the following steps:

[0033] 12.5 g of calcium chloride and 11 g of disodium hydrogen phosphate were added to 50 wt% ammonia water, mixed uniformly, stirred for 1 h, centrifuged, washed, and hydroxyapatite was obtained. The hydroxyapatite was dispersed in anhydrous ethanol, 6 g of phosphatidylserine was added, stirred for 2 h, 21 g of polyethyleneimine was continuously added, the temperature was controlled at 50°C, and the reaction was continued for 4 h. After centrifugation, washing, and drying, polyethyleneimine-loaded phosphatidylserine-modified hydroxyapatite, i.e., a modified carrier, was prepared.

[0034] Example 3

[0035] The modified carrier was prepared by the following steps:

[0036] 15 g of calcium chloride and 14 g of disodium hydrogen phosphate were added to 50 wt% ammonia water, mixed uniformly, stirred for 1 h, centrifuged, washed, and hydroxyapatite was obtained. The hydroxyapatite was dispersed in anhydrous ethanol, 7 g of phosphatidylserine was added, stirred for 2 h, 22 g of polyethyleneimine was continuously added, the temperature was controlled at 50°C, and the reaction was continued for 4 h. After centrifugation, washing, and drying, polyethyleneimine-loaded phosphatidylserine-modified hydroxyapatite, i.e., a modified carrier, was prepared.

[0037] Example 4

[0038] A liquid microbial agent application method suitable for different crops includes the following steps:

[0039] S1, dissolving the microbial agent in 50 wt% water to obtain a liquid microbial agent;

[0040] S2, selecting a target crop, determining the fertilization requirement according to its growth stage, and uniformly applying the liquid microbial agent prepared in Example 1 to the soil at the root of the target crop. Within 7 days after the target crop germinates, the fertilization frequency is once every 2 days, and each time 5 g / m 2 of the liquid microbial agent prepared in Example 1 is applied. During the seedling growth period, the fertilization frequency is once every 1 day, and each time 10 g / m 2, and 40 g / m of the liquid microbial inoculant prepared in Example 2 is applied every 7 days from before flowering to the end of the flowering period 2 , and 30 g / m of the liquid microbial inoculant prepared in Example 2 is applied every 5 days during the fruiting period 2 .

[0041] S3, field management is performed, regular irrigation is performed to control the soil humidity to be 60%, regular weeding is performed, and diseases and pests are controlled in a timely manner.

[0042] Example 5

[0043] A liquid microbial inoculant application method applicable to different crops includes the following steps:

[0044] S1, dissolving the microbial inoculant in 50wt% water to obtain a liquid microbial inoculant;

[0045] S2, selecting a target crop, determining the fertilization requirement according to the growth stage, and uniformly applying the liquid microbial inoculant prepared in Example 2 to the root soil of the target crop, the fertilization frequency being 7.5 g / m of the liquid microbial inoculant prepared in Example 2 every 2 days within 7 days after the target crop germinates 2 , the fertilization frequency being 15 g / m of the liquid microbial inoculant prepared in Example 2 every 1 day during the seedling growth period 2 , the fertilization frequency being 40 g / m of the liquid microbial inoculant prepared in Example 2 every 7 days from before flowering to the end of the flowering period 2 , and the fertilization frequency being 30 g / m of the liquid microbial inoculant prepared in Example 2 every 5 days during the fruiting period 2 .

[0046] S3, field management is performed, regular irrigation is performed to control the soil humidity to be 60%, regular weeding is performed, and diseases and pests are controlled in a timely manner.

[0047] Example 6

[0048] A liquid microbial inoculant application method applicable to different crops includes the following steps:

[0049] S1, dissolving the microbial inoculant in 50wt% water to obtain a liquid microbial inoculant;

[0050] S2, selecting a target crop, determining the fertilization requirement according to the growth stage, and uniformly applying the liquid microbial inoculant prepared in Example 3 to the root soil of the target crop, the fertilization frequency being 10 g / m of the liquid microbial inoculant prepared in Example 3 every 2 days within 7 days after the target crop germinates 2 , the fertilization frequency being 20 g / m of the liquid microbial inoculant prepared in Example 3 every 1 day during the seedling growth period 2, the flowering period, every 7d, each time applying the liquid microbial inoculum prepared in Example 3 50g / m 2 , the flowering period, every 7d, each time applying the liquid microbial inoculum prepared in Example 3 50g / m 2 .

[0051] S3, field management, regular irrigation, control the soil humidity to 60%, regular weeding, and timely control of diseases and pests.

[0052] Comparative Example 1

[0053] In this comparative example, no modified carrier is used, only microbial inoculum is used, and the rest of the steps are the same as Example 6, i.e.:

[0054] S1, dissolve the microbial inoculum in 50wt% water to obtain a liquid microbial inoculum;

[0055] S2, select the target crop, determine the fertilization requirement according to its growth stage, and uniformly apply the microbial inoculum to the soil at the root of the target crop, the fertilization frequency is every 2d within 7d after the target crop germination, each time applying the microbial inoculum 10g / m 2 , the fertilization frequency is every 1d during the seedling growth period, each time applying the microbial inoculum 20g / m 2 , the flowering period, every 7d, each time applying the liquid microbial inoculum prepared in Example 3 50g / m 2 , the flowering period, every 7d, each time applying the liquid microbial inoculum prepared in Example 3 50g / m 2 .

[0056] S3, field management, regular irrigation, control the soil humidity to 60%, regular weeding, and timely control of diseases and pests.

[0057] Select a test field, plow and level the soil, divide it into several plots, each plot area is 10mx10m, plant the same target crop wheat in each plot, seed according to the normal planting density, ensure the number of crops in each plot is consistent, according to the liquid microbial inoculum application method in Examples 4-6 and Comparative Example 1, set up another group of blank control, fertilize each plot at different stages, observe and record the growth status of crops at different growth stages, the results are shown in Table 1:

[0058] Table 1. Wheat growth status of Examples 4-6 and Comparative Example 1

[0059]

[0060] According to the same method, replace the target crop with corn for testing, fertilize each plot at different stages, observe and record the growth status of crops at different growth stages, the results are shown in Table 2:

[0061] Table 2. Corn growth conditions of Examples 4-6 and Comparative Example 1

[0062]

[0063]

[0064] According to the same method, the target crop was replaced with soybeans, and each plot was fertilized at different stages. The growth conditions of the crops were observed and recorded at different growth stages, and the results are shown in Table 3:

[0065] Table 3. Soybean growth conditions of Examples 4-6 and Comparative Example 1

[0066]

[0067]

[0068] As can be seen from Table 1, the liquid microbial agent application methods in Examples 4-6 significantly improved the growth conditions of wheat. Compared with Comparative Example 1 and the blank control, the average plant height and average yield were significantly increased. In addition, the soil organic matter content was also increased, indicating that the modified carrier had a significant synergistic effect on the microbial agent.

[0069] As can be seen from Table 2, the liquid microbial agent application methods in Examples 4-6 performed similarly on corn as on wheat, with significant increases in average plant height and average yield, and an increase in soil organic matter content. Compared with Comparative Example 1 and the blank control, the modified carrier also had a significant synergistic effect on the microbial agent.

[0070] As can be seen from Table 3, the liquid microbial agent application methods in Examples 4-6 performed slightly worse on soybeans than on wheat and corn, but still showed some synergistic effect. The average yields of Examples 4-6 were 3.85 kg / m 2 , 3.89 kg / m 2 , and 4.24 kg / m 2 , respectively, which were higher than those of Comparative Example 1 and the blank control. The average plant height and average yield were increased, and the soil organic matter content was also increased.

[0071] In summary, the liquid microbial agent application methods in Examples 4-6 showed some synergistic effect on different crops, with the modified carrier playing a key role. In contrast, Comparative Example 1, which did not use a modified carrier, performed poorly in terms of crop growth conditions and soil organic matter content. Therefore, the modified carrier can improve the fertilizer efficiency of the microbial agent, promote the growth of crops, and increase the soil organic matter content.

[0072] In the description, references to "one embodiment," "an example," "certain examples" etc. mean that the particular feature, structure, material, or characteristic being described is included in at least one embodiment or example of the application. The appearances of an item in various examples or embodiments is not necessarily indicative of a frequency of occurrence in the various examples or embodiments. Moreover, descriptions of well-known methods associated are omitted so as not to obscure the disclosure.

[0073] The foregoing merely illustrates the principles of the application. Various modifications and adaptations will occur to those skilled in the art after consideration of the preceding description. All such modifications and adaptations employing the principles of the application are intended to be within the scope of the claims.

Claims

1. A method of applying liquid microbial inoculant applicable to different crops, characterized in that, The method comprises the following steps: S1, dissolving the microbial agent in 50-90wt% water to obtain a liquid microbial agent; S2, selecting a target crop, determining the fertilization requirement according to the growth stage, and uniformly applying the liquid microbial agent to the soil at the root of the target crop; S3, performing field management, regularly irrigating, controlling the soil humidity to be 60-80%, regularly weeding, and timely preventing and treating diseases and pests; The microbial agent in step S1 comprises the following raw materials in parts by weight: 5-7 parts of active microbial strains, 10-20 parts of modified carriers, 30-40 parts of water-soluble organic fertilizers, 1-3 parts of protective agents, and 2-4 parts of ethylenediaminetetraacetic acid; The protective agent comprises one or more of trehalose, polyethylene glycol, carboxymethyl cellulose, glycine, and proline; The modified carrier is prepared by the following steps: Calcium chloride and disodium hydrogen phosphate are added to 50-60wt% ammonia water, mixed uniformly, stirred and reacted for 1-2h, centrifuged, washed, and hydroxyapatite is obtained, the hydroxyapatite is dispersed in anhydrous ethanol, phosphatidylserine is added, stirred and reacted for 2-4h, polyethyleneimine is continuously added, the temperature is controlled at 50-60℃, and the reaction is continuously carried out for 4-5h, centrifuged, washed, and dried to obtain polyethyleneimine loaded phosphatidylserine modified hydroxyapatite, i.e., the modified carrier.

2. The method of claim 1, wherein the liquid microbial inoculant is applied to different crops. The fertilization requirement in step S2 is specifically: A1, within 7-14 days after the target crop germination, the fertilization frequency is once every 2-3 days, and the liquid microbial agent is applied 5-10 g / m each time 2 ; A2, the frequency of fertilization during the seedling growth period is once every 1-2 days, and 10-20 g / m of liquid microbial agent is applied each time 2 ; A3, before flowering to the end of flowering period, once every 7d, 30-50g / m of liquid microbial inoculant was applied each time 2 ; A3, once every 5-7 days during the results period, each time applying 20-40 g / m of liquid microbial inoculant 2 .

3. The method of claim 1, wherein the liquid microbial inoculant is applied to different crops. The active microbial strain suspension is cultured for 3-5d and then inoculated on the modified carrier, and the active microbial strain comprises one or more of Bacillus subtilis, lactic acid bacteria, actinomycetes, Bacillus megaterium, and rhizobium.

4. The method of claim 1, wherein the liquid microbial inoculum is applied to different crops. The water-soluble organic fertilizer comprises the following raw materials in parts by weight: 10-20 parts of humic acid, 40-60 parts of organic matter, 10-14 parts of urea, 7-9 parts of potassium dihydrogen phosphate, and 5-7 parts of potassium nitrate.

5. The method of claim 1, wherein the liquid microbial inoculum is applied to different crops. The mass ratio of calcium chloride, disodium hydrogen phosphate, phosphatidylserine, and polyethyleneimine is (10-15):(8-14):(5-7):(20-22).

Images