A composition for reducing uric acid and a preparation method and application thereof

Abstract

The application belongs to the technical field of traditional Chinese medicine health care, and particularly relates to a composition for reducing high uric acid, a preparation method and application thereof. The composition for reducing high uric acid is composed of the following components in parts by weight: 10-25 parts of Pingshuqiu Sanqi, 10-15 parts of Pueraria, 8-12 parts of sweet leaf chrysanthemum, 5-15 parts of Eucommia ulmoides leaf, and 0.1-3 parts of fruit and vegetable powder. The application obtains a composition for reducing high uric acid by scientifically proportioning Pingshuqiu Sanqi, Pueraria, sweet leaf chrysanthemum, Eucommia ulmoides leaf and fruit and vegetable powder. The composition formula does not produce toxic side effects after long-term use, is safer to use, and provides a preparation method of the composition for reducing high uric acid, which is low in production cost and effectively saves energy. The total flavonoid content of the prepared composition for reducing high uric acid is 10.88 mg / g on average, and the transfer rate of total flavonoids is 89.48%, which is good in extraction effect.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine health care technology, specifically relating to a composition for lowering high uric acid, its preparation method, and its application. Background Technology

[0002] Hyperuricemia (HUA) refers to excessive uric acid production and / or insufficient uric acid excretion in the body under normal dietary conditions. Hyperuricemia is defined as a fasting blood uric acid level exceeding 420 μmol / L in men and 360 μmol / L in women on two separate occasions. In recent years, due to changes in lifestyle, the intake of high-protein and high-fat diets, as well as unhealthy habits such as excessive alcohol consumption, staying up late, and lack of exercise, the incidence of hyperuricemia is increasing and showing a trend towards affecting younger people. Clinically, febuxostat and allopurinol, which inhibit uric acid production, and probenecid and benzbromarone, which promote uric acid excretion, are commonly used to lower blood uric acid levels. However, long-term use can produce multiple side effects, such as gastrointestinal discomfort, skin rashes, bone marrow suppression, and liver and kidney damage, affecting its widespread clinical application. Therefore, further research and development of drug formulations that are effective in lowering blood uric acid with fewer toxic side effects is needed. Summary of the Invention

[0003] The purpose of this invention is to address the shortcomings of existing technologies by providing a composition for lowering uric acid, its preparation method, and its application. The invention scientifically combines Panax notoginseng, kudzu root, stevia, Eucommia ulmoides leaves, and fruit and vegetable powders to obtain a composition for lowering uric acid. This composition is safe to use and will not produce toxic side effects with long-term use. Furthermore, the invention provides a method for preparing this uric acid-lowering composition that is low in production cost and effectively saves energy.

[0004] To solve the above-mentioned technical problems of the present invention, the present invention provides the following technical solution:

[0005] One of the objectives of this invention is to provide a composition for lowering uric acid, comprising the following components in parts by weight: 10-25 parts of Panax notoginseng, 10-15 parts of Pueraria lobata, 8-12 parts of Stevia rebaudiana, 5-15 parts of Eucommia ulmoides leaves, and 0.1-3 parts of fruit and vegetable powder.

[0006] Gynostemma pentaphyllum is neutral in nature and has a sweet and bland taste. It is a unique perennial herbaceous plant belonging to the genus Gynostemma of the Asteraceae family that can be used for both medicinal and edible purposes. It contains active ingredients such as organic calcium, neochlorogenic acid, flavonoids, alkaloids, and sesquiterpenes. Gynostemma pentaphyllum has the effects of promoting blood circulation, relieving inflammation and cough, dispersing blood stasis and reducing swelling, and promoting blood circulation and tissue regeneration. It is mainly used to treat traumatic injuries, rheumatic joint pain, and gout.

[0007] Kudzu root is an important natural plant in my country that is both food and medicine, and is known as "southern kudzu and northern ginseng". It contains isoflavone compounds such as puerarin, daidzein, and daidzin, which have a variety of pharmacological effects, including relieving muscle tension and fever, promoting body fluid production and quenching thirst, promoting rash eruption, raising yang and stopping diarrhea, clearing the meridians and activating collaterals, and detoxifying alcohol.

[0008] Stevia is the leaf of the stevia plant, belonging to the genus Stevia in the family Asteraceae. Native to the Amanbai Mountains on the border of Paraguay and Brazil in South America, it was initially used by locals to mask the bitterness of herbs. Stevia was successfully introduced from Japan in 1976. Currently, my country is the world's largest producer and exporter of stevia, with cultivation in Beijing, Hebei, Shaanxi, Jiangsu, Fujian, Hunan, Yunnan, and other regions. It is widely used in the food, pharmaceutical, and cosmetic industries. In recent years, scholars both domestically and internationally have discovered that stevia extract possesses pharmacological activities such as antioxidant, antibacterial, antiviral, antitumor, adjuvant treatment of diabetes, regulation of lipid metabolism, and immunomodulation.

[0009] Eucommia ulmoides leaves are the dried leaves of the Eucommia ulmoides plant, belonging to the Eucommiaceae family. They are a dual-purpose medicinal and edible ingredient, sharing the same active ingredients and pharmacological effects as Eucommia ulmoides bark. More than 70 organic compounds have been isolated and identified, including at least 15 inorganic mineral elements, which can be broadly categorized into iridoids, lignans, flavonoids, eucommia gum, phenylpropanoids, phenols, amino acids, polysaccharides, fatty acids, and vitamins. Eucommia ulmoides leaves are slightly pungent and warm in nature. They enter the liver and kidney meridians, and have the functions of tonifying the liver and kidneys, and strengthening tendons and bones. They are used for liver and kidney deficiency, dizziness, lower back and knee pain, and weakness of tendons and bones.

[0010] Fruit and vegetable powder is made from fresh fruits and vegetables through more than 10 processes, including pretreatment, quick-freezing, vacuum drying, ultraviolet sterilization, packaging, and storage, using internationally advanced freeze-drying technology and equipment. It can be applied to almost all areas of food processing, and can be used to improve the nutritional content, color, and flavor of products.

[0011] This invention scientifically combines Panax notoginseng, kudzu root, stevia, Eucommia ulmoides leaves, and fruit and vegetable powder to obtain a composition for lowering uric acid. In this formula, Panax notoginseng, kudzu root, and Eucommia ulmoides leaves are all medicinal and edible raw materials, while stevia and fruit and vegetable powder are food raw materials. This composition will not produce toxic side effects with long-term use, is relatively safe to use, and has a good function of lowering uric acid.

[0012] Furthermore, the fruit and vegetable powder is at least one of prickly pear powder, apple powder, and acerola cherry powder.

[0013] A second objective of this invention is to provide a method for preparing a composition for lowering uric acid, comprising the following steps:

[0014] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take the prescribed amount of Panax notoginseng, kudzu root, stevia, and eucommia leaves and pulverize them.

[0015] Take the pulverized material, add mixed enzyme and purified water to obtain a mixed solution, add pH adjuster to adjust pH to 4.0-5.0, and then perform variable frequency ultrasound-assisted enzymatic extraction at 40-50℃. First, treat with low frequency ultrasound at 30-40kHz for 10-15min, and then treat with high frequency ultrasound at 70-80kHz for 15-20min; boil for 5-6min to inactivate the enzyme and obtain the extract.

[0016] The obtained extract was filtered through 200-300 mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. 3-5 times the volume of 50%-70% ethanol was added to the concentrate for alcohol precipitation, centrifugation, and freeze drying to obtain the compound powder of traditional Chinese medicine extract.

[0017] A compound powder of traditional Chinese medicine extracts and fruit and vegetable powders are mixed to obtain a composition that lowers uric acid.

[0018] Further, the mixed enzyme is cellulase R-10 and analyte R-10, wherein the mass ratio of cellulase R-10 to analyte R-10 is 2-3:1, and the mixed enzyme accounts for 1%-2% of the total weight of the mixture.

[0019] Cellulase R-10 is a type of cellulase derived from *Trichoderma viride*. It is an enzyme mixture comprising cellulase, hemicellulase, pectinase, and protease. Isolase R-10 is a macerating enzyme derived from *Rhizopus*, containing high pectinase and hemicellulase activity. Combining cellulase R-10 and isolase R-10 with variable-frequency ultrasound-assisted enzymatic hydrolysis ensures thorough contact between the mixed enzymes and the raw medicinal material. This enzymatic hydrolysis effectively disrupts the cell wall structure of the raw material, and the variable-frequency ultrasound further facilitates the release of the active ingredients. Traditionally, large-scale industrial production of raw medicinal materials typically involves 2-3 cycles of water decoction, resulting in high water and energy consumption and production costs. This invention addresses this by subjecting the raw medicinal materials to frequency-modulated ultrasonic-assisted enzymatic extraction at a pH of 4.0-5.0 and a temperature of 40-50°C. This significantly reduces the production cost of raw medicinal material extraction. Furthermore, under the action of mixed enzymes, the average total flavonoid content of the active ingredient is 10.88 mg / g, with a total flavonoid transfer rate of 89.48%, demonstrating excellent extraction results.

[0020] Furthermore, the pH adjuster is at least one of citric acid, lactic acid, and malic acid. By using the pH adjuster to adjust the pH of the enzymatic hydrolysis environment to between 4.0 and 5.0, the enzymatic activities of cellulase R-10 and dissociative enzyme R-10 can be fully utilized.

[0021] Furthermore, the mass ratio of the pulverized material to purified water is 1:6-10.

[0022] Furthermore, the centrifugation speed is 4000-6000 rpm, and the centrifugation time is 3-5 min.

[0023] A third objective of this invention is to provide the application of the above-mentioned uric acid-lowering composition in the preparation of drugs, health foods, or functional foods for the prevention and treatment of hyperuricemia.

[0024] The beneficial effects of this invention are:

[0025] 1. This invention scientifically combines Panax notoginseng, kudzu root, stevia, Eucommia ulmoides leaves, and fruit and vegetable powder to obtain a composition for lowering uric acid. In this formula, Panax notoginseng, kudzu root, and Eucommia ulmoides leaves are all medicinal and edible raw materials, while stevia and fruit and vegetable powder are food raw materials. This composition will not produce toxic side effects with long-term use, is relatively safe to use, and has a good function of lowering uric acid.

[0026] 2. This invention also provides a method for preparing a low-cost, energy-saving composition for lowering uric acid. Traditionally, large-scale industrial production of raw medicinal materials typically involves 2-3 water decoctions, resulting in high water and energy consumption and production costs. This invention involves enzymatic extraction of the raw medicinal materials at pH 4.0-5.0 and 40-50℃. Cellulase R-10 and analyte R-10 are used in combination, with variable-frequency ultrasound assisting the enzymatic extraction to ensure sufficient contact between the mixed enzymes and the raw medicinal materials. Enzymatic extraction effectively disrupts the cell wall structure of the raw medicinal materials, and the variable-frequency ultrasound assists in the full release of the active ingredients, effectively reducing the production cost of raw medicinal material extraction. Simultaneously, under the action of the mixed enzymes, the average total flavonoid content of the raw medicinal materials is 10.88 mg / g, and the total flavonoid transfer rate is 89.48%, indicating good extraction results. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with specific embodiments in the specification. In the present invention, unless otherwise specified, all raw materials, equipment, and instruments used in the preparation are commercially available products well-known to those skilled in the art.

[0028] The present invention provides a composition for lowering high uric acid, comprising the following components in parts by weight: 10-25 parts of Panax notoginseng, 10-15 parts of Pueraria lobata, 8-12 parts of Stevia rebaudiana, 5-15 parts of Eucommia ulmoides leaves, and 0.1-3 parts of fruit and vegetable powder.

[0029] Preferably, in this invention, the composition for lowering uric acid consists of the following components in parts by weight: 20 parts of Panax notoginseng, 12 parts of kudzu root, 10 parts of stevia, 10 parts of Eucommia ulmoides leaves, and 1 part of fruit and vegetable powder.

[0030] In this formula, Panax notoginseng, kudzu root, and Eucommia leaves are all medicinal and edible ingredients, while Stevia and fruit and vegetable powders are food ingredients. This combination formula will not produce toxic side effects with long-term use, is relatively safe to use, and has a good function of lowering uric acid.

[0031] In this invention, the fruit and vegetable powder is at least one of prickly pear powder, apple powder, and acerola cherry powder.

[0032] The present invention also provides a method for preparing a composition for lowering uric acid, comprising the following steps:

[0033] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take the prescribed amount of Panax notoginseng, kudzu root, stevia, and eucommia leaves and pulverize them.

[0034] Take the pulverized material, add mixed enzyme and purified water to obtain a mixed solution, add pH adjuster to adjust pH to 4.0-5.0, and then perform variable frequency ultrasound-assisted enzymatic extraction at 40-50℃. First, treat with low frequency ultrasound at 30-40kHz for 10-15min, and then treat with high frequency ultrasound at 70-80kHz for 15-20min; boil for 5-6min to inactivate the enzyme and obtain the extract.

[0035] The obtained extract was filtered through 200-300 mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. 3-5 times the volume of 50%-70% ethanol was added to the concentrate for alcohol precipitation, centrifugation, and freeze drying to obtain the compound powder of traditional Chinese medicine extract.

[0036] A compound powder of traditional Chinese medicine extracts and fruit and vegetable powders are mixed to obtain a composition that lowers uric acid.

[0037] Among them, the leaves of Panax notoginseng, kudzu root, stevia, and Eucommia ulmoides can be pulverized and then sieved. The sieve mesh size is 40-60 mesh, preferably 60 mesh.

[0038] In this invention, when the pulverized material is added to the mixed enzyme and purified water to obtain a mixed solution, it is preferable to first mix the purified water with the pulverized material and then add the mixed enzyme. This can effectively avoid the environmental influence causing the mixed enzyme to lose its activity. After obtaining the mixed solution, a pH adjuster is added to adjust the pH of the mixed solution to 4.0-5.0. The pH adjuster is preferably added dropwise, with pH 4.8 being the preferred pH. Under pH 4.8 conditions, the mixed enzyme has better enzyme activity.

[0039] When performing enzymatic hydrolysis of the mixture, it is preferable to conduct it in a constant-temperature water bath to maintain a constant hydrolysis temperature. The optimal hydrolysis temperature is 45℃, at which point the enzyme activity of the mixed enzyme is better, resulting in a better hydrolysis effect. During enzymatic hydrolysis, variable-frequency ultrasound is used to assist in the extraction. The high-energy kinetic energy generated by the ultrasound allows the mixed enzyme to gain kinetic energy and make full contact with the raw materials, resulting in more complete hydrolysis, disrupting the cell wall structure of the original medicinal materials, and accelerating the migration rate of the active ingredients in the original medicinal materials, thereby achieving the purpose of extraction.

[0040] Preferably, during the frequency-modulated ultrasound-assisted enzymatic extraction, the process involves first treating with low-frequency ultrasound at 35 kHz for 12 minutes, followed by high-frequency ultrasound at 75 kHz for 18 minutes; then boiling for 5 minutes to inactivate the enzyme, yielding the extract. In large-scale industrial production, extraction typically involves 2-3 cycles of water decoction, resulting in high water and energy consumption and production costs. This invention utilizes enzymatic hydrolysis combined with frequency-modulated ultrasound treatment, effectively reducing production costs and improving the extraction efficiency of the total flavonoids, the active ingredient.

[0041] The obtained extract is filtered through 200-300 mesh gauze, preferably 300 mesh, to effectively remove filter residue. The filtrate is concentrated under reduced pressure to 1 / 3-1 / 2 of its original volume. Then, 3-5 times the volume of 50%-70% ethanol is added to the concentrate for alcohol precipitation, followed by centrifugation and freeze-drying to obtain a compound powder of the traditional Chinese medicine extract. Alcohol precipitation is preferably performed using 4 times the volume of 60% ethanol, which effectively collects the total flavonoids, the active ingredient, from the enzymatic hydrolysate. Centrifugation and freeze-drying can be performed according to procedures well known to those skilled in the art.

[0042] In this invention, the mixed enzyme is cellulase R-10 and cleavage enzyme R-10, wherein the mass ratio of cellulase R-10 to cleavage enzyme R-10 is 2-3:1, and the mixed enzyme accounts for 1%-2% of the total weight of the mixture. Preferably, the mass ratio of cellulase R-10 to cleavage enzyme R-10 is 2:1, and the mixed enzyme accounts for 1.5% of the total weight of the mixture. The cell walls of the original medicinal plant mainly consist of cellulose, hemicellulose, lignin, pectin, mucopolysaccharides, etc., and the composition is relatively complex. Selecting a single enzyme for enzymatic hydrolysis usually cannot effectively destroy the cell walls of the original medicinal plant. Therefore, this invention selects cellulase R-10 and cleavage enzyme R-10 for combined use among many enzyme types, which has a better enzymatic hydrolysis effect.

[0043] In this invention, the pH adjuster is at least one of citric acid, lactic acid, and malic acid.

[0044] In this invention, the mass ratio of the pulverized material to purified water is 1:6-10. Preferably, the mass ratio of the pulverized material to purified water is 1:8. The amount of purified water added should not be too much or too little. If too much purified water is added, the enzymatic contact will be insufficient, and the subsequent concentration will take longer, which is not conducive to saving production costs. If too little purified water is added, the pulverized material cannot be sufficiently dispersed, which is not conducive to the full enzymatic hydrolysis.

[0045] In this invention, the centrifugation speed is 4000-6000 rpm and the centrifugation time is 3-5 min.

[0046] The present invention also provides the application of the above-mentioned composition for lowering uric acid in the preparation of drugs, health foods or functional foods for the prevention and treatment of hyperuricemia.

[0047] To further illustrate the present invention, the following detailed description, in conjunction with examples, provides a composition for lowering uric acid, its preparation method, and its application, but these descriptions should not be construed as limiting the scope of protection of the present invention.

[0048] Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods. The invention is further illustrated below with reference to the embodiments:

[0049] Example 1: Preparation of a composition for lowering uric acid

[0050] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take 20 parts of Panax notoginseng, 12 parts of kudzu root, 10 parts of stevia, and 10 parts of eucommia leaves and pulverize them.

[0051] Take the pulverized material, add 8 times the amount of purified water, cellulase R-10, and analyte R-10 to obtain a mixture, wherein the amount of cellulase R-10 added is 1.0% of the total amount of the mixture, and the amount of analyte R-10 added is 0.5% of the total amount of the mixture. After adjusting the pH to 4.8 with citric acid, perform frequency-modulated ultrasonic-assisted enzymatic extraction at 45℃. First, treat with low-frequency ultrasonic at 35kHz for 12min, and then treat with high-frequency ultrasonic at 75kHz for 18min. Boil for 5min to inactivate the enzymes and obtain the extract.

[0052] The obtained extract was filtered through 300-mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. Four times the volume fraction of 60% ethanol was added to the concentrate for precipitation. After centrifugation at 5000 rpm for 5 min, the extract was freeze-dried to obtain the compound powder of traditional Chinese medicine extract.

[0053] A mixture of one part of traditional Chinese medicine extract powder and one part of acerola cherry powder was prepared to obtain a composition for lowering uric acid.

[0054] Example 2: Preparation of a composition for lowering uric acid

[0055] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take 10 parts of Panax notoginseng, 15 parts of kudzu root, 8 parts of stevia, and 15 parts of eucommia leaves and pulverize them.

[0056] Take the pulverized material, add 10 times the amount of purified water, cellulase R-10, and analyte R-10 to obtain a mixture, wherein the amount of cellulase R-10 added is 1.5% of the total amount of the mixture, and the amount of analyte R-10 added is 0.5% of the total amount of the mixture. After adjusting the pH to 4.0 with citric acid, perform frequency-modulated ultrasonic-assisted enzymatic extraction at 40℃. First, treat with low-frequency ultrasonic at 40kHz for 10min, and then treat with high-frequency ultrasonic at 80kHz for 18min. Boil for 6min to inactivate the enzymes and obtain the extract.

[0057] The obtained extract was filtered through 200-mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. Five times the volume fraction of 50% ethanol was added to the concentrate for alcohol precipitation. After centrifugation at 4000 rpm for 5 min, the extract was freeze-dried to obtain the compound powder of traditional Chinese medicine extract.

[0058] Three parts of compound powder of traditional Chinese medicine extract and apple powder were mixed to obtain a composition for lowering uric acid.

[0059] Example 3: Preparation of a composition for lowering uric acid

[0060] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take 25 parts of Panax notoginseng, 10 parts of kudzu root, 12 parts of stevia, and 5 parts of eucommia leaves and pulverize them.

[0061] Take the pulverized material, add 6 times the amount of purified water, cellulase R-10, and analyte R-10 to obtain a mixture, wherein the amount of cellulase R-10 added is 1.2% of the total amount of the mixture, and the amount of analyte R-10 added is 0.6% of the total amount of the mixture. After adjusting the pH to 5.0 with citric acid, perform frequency-modulated ultrasonic-assisted enzymatic extraction at 50℃. First, treat with low-frequency ultrasonic at 30kHz for 15min, and then treat with high-frequency ultrasonic at 70kHz for 20min. Boil for 6min to inactivate the enzymes and obtain the extract.

[0062] The obtained extract was filtered through 200-mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. Three times the volume fraction of 70% ethanol was added to the concentrate for alcohol precipitation. After centrifugation at 6000 rpm for 4 min, the extract was freeze-dried to obtain the compound powder of traditional Chinese medicine extract.

[0063] Two parts of compound powder of traditional Chinese medicine extract and prickly pear powder were mixed to obtain a composition for lowering uric acid.

[0064] Comparative Example 1: The same as Example 1, but without the use of dissociative enzyme R-10.

[0065] Comparative Example 2: The same as Example 1, but without cellulase R-10.

[0066] Comparative Example 3:

[0067] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take 20 parts of Panax notoginseng, 12 parts of kudzu root, 10 parts of stevia, and 10 parts of eucommia leaves and pulverize them.

[0068] Take the pulverized material, add 8 times the amount of purified water, cellulase R-10, and analyte R-10 to obtain a mixture, wherein the amount of cellulase R-10 added is 1.0% of the total amount of the mixture, and the amount of analyte R-10 added is 0.5% of the total amount of the mixture. After adjusting the pH to 4.8 with citric acid, enzymatic hydrolysis is performed at 45℃ for 30 min; the enzyme is then inactivated by boiling for 5 min to obtain the extract.

[0069] The obtained extract was filtered through 300-mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. Four times the volume fraction of 60% ethanol was added to the concentrate for precipitation. After centrifugation at 5000 rpm for 5 min, the extract was freeze-dried to obtain the compound powder of traditional Chinese medicine extract.

[0070] A mixture of one part of traditional Chinese medicine extract powder and one part of acerola cherry powder was prepared to obtain a composition for lowering uric acid.

[0071] Comparative Example 4:

[0072] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take 20 parts of Panax notoginseng, 12 parts of kudzu root, 10 parts of stevia, and 10 parts of eucommia leaves and pulverize them.

[0073] Take the pulverized material, add 8 times the amount of purified water, cellulase R-10, and analyte R-10 to obtain a mixture, wherein the amount of cellulase R-10 added is 1.0% of the total amount of the mixture, and the amount of analyte R-10 added is 0.5% of the total amount of the mixture. After adjusting the pH to 4.8 with citric acid, perform ultrasonic-assisted enzymatic hydrolysis extraction at 45℃ with an ultrasonic frequency of 35kHz for 30 min; boil for 5 min to inactivate the enzymes and obtain the extract.

[0074] The obtained extract was filtered through 300-mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. Four times the volume fraction of 60% ethanol was added to the concentrate for precipitation. After centrifugation at 5000 rpm for 5 min, the extract was freeze-dried to obtain the compound powder of traditional Chinese medicine extract.

[0075] A mixture of one part of traditional Chinese medicine extract powder and one part of acerola cherry powder was prepared to obtain a composition for lowering uric acid.

[0076] Comparative Example 5:

[0077] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take 20 parts of Panax notoginseng, 12 parts of kudzu root, 10 parts of stevia, and 10 parts of eucommia leaves and pulverize them.

[0078] Take the pulverized material, add 8 times the amount of purified water, cellulase R-10, and analyte R-10 to obtain a mixture, wherein the amount of cellulase R-10 added is 1.0% of the total amount of the mixture, and the amount of analyte R-10 added is 0.5% of the total amount of the mixture. After adjusting the pH to 4.8 with citric acid, perform ultrasonic-assisted enzymatic hydrolysis extraction at 45℃ with an ultrasonic frequency of 75kHz for 30 min; boil for 5 min to inactivate the enzyme, and obtain the extract.

[0079] The obtained extract was filtered through 300-mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. Four times the volume fraction of 60% ethanol was added to the concentrate for precipitation. After centrifugation at 5000 rpm for 5 min, the extract was freeze-dried to obtain the compound powder of traditional Chinese medicine extract.

[0080] A mixture of one part of traditional Chinese medicine extract powder and one part of acerola cherry powder was prepared to obtain a composition for lowering uric acid.

[0081] Comparative Example 6:

[0082] After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take 20 parts of Panax notoginseng, 12 parts of kudzu root, 10 parts of stevia, and 10 parts of eucommia leaves and pulverize them.

[0083] Take the pulverized material, add 8 times the amount of purified water, cellulase R-10, and analyte R-10 to obtain a mixture, wherein the amount of cellulase R-10 added is 1.0% of the total amount of the mixture, and the amount of analyte R-10 added is 0.5% of the total amount of the mixture. After adjusting the pH to 4.8 with citric acid, perform ultrasonic-assisted enzymatic hydrolysis extraction at 45℃ with an ultrasonic frequency of 75kHz for 30 min; boil for 5 min to inactivate the enzyme, and obtain the extract.

[0084] The obtained extract was filtered through 300-mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. After centrifugation at 5000 rpm for 5 min, it was freeze-dried to obtain the compound powder of traditional Chinese medicine extract.

[0085] A mixture of one part of traditional Chinese medicine extract powder and one part of acerola cherry powder was prepared to obtain a composition for lowering uric acid.

[0086] Experimental Example 1:

[0087] Calculate the total flavonoid content and the conversion rate of total flavonoids in the high-uric-acid-lowering compositions prepared in Examples 1-3 and Comparative Examples 1-6. The test method for total flavonoids refers to "Determination of Total Flavonoids in Health Foods" in the "Technical Guidelines for Inspection and Evaluation of Physical and Chemical and Hygiene Indicators of Health Foods (2020 Edition)". The calculation formula for the conversion rate of total flavonoids is: Total flavonoid transfer rate (%) = Total flavonoid content in the extract / Total flavonoid content in the medicinal materials × 100%. The results are shown in Table 1.

[0088] Table 1

[0089] Group Total flavonoid content, mg / g Total flavonoid transfer rate, % Example 1 11.34 90.24 Example 2 10.25 88.63 Example 3 11.07 89.58 Comparative Example 1 6.46 50.37 Comparative Example 2 7.15 56.91 Comparative Example 3 8.32 65.38 Comparative Example 4 9.50 76.12 Comparative Example 5 9.88 78.49 Comparative Example 6 9.39 73.73

[0090] As can be seen from Table 1, the average total flavonoid content of the high-uric-acid-lowering compositions prepared in Examples 1-3 is 10.88 mg / g, and the transfer rate of total flavonoids is 89.48%. It can be seen that the extraction rate of total flavonoids is relatively high.

[0091] After not adding macerozyme R-10 and cellulase R-10 in Comparative Examples 1 and 2 respectively, the total flavonoid contents of the high-uric-acid-lowering compositions prepared are 6.46 mg / g and 7.15 mg / g respectively, and the transfer rates of total flavonoids are 50.37% and 56.91% respectively, indicating that macerozyme R-10 and cellulase R-10 have a good synergistic effect, and the total flavonoid content of the high-uric-acid-lowering compositions prepared is relatively high.

[0092] After not using ultrasonic-assisted enzymatic hydrolysis in Comparative Example 3, the total flavonoid content of the high-uric-acid-lowering composition prepared is 8.32 mg / g, and the transfer rate of total flavonoids is 65.38%, which is lower than that of Example 1 and Comparative Examples 4 and 5, indicating that enzymatic hydrolysis assisted by variable-frequency ultrasound has a good extraction effect.

[0093] After not using alcohol precipitation in Comparative Example 6, the total flavonoid content of the high-uric-acid-lowering composition prepared is 9.39 mg / g, and the transfer rate of total flavonoids is 73.73%, indicating that alcohol precipitation can effectively collect the active ingredient total flavonoids in the enzymatic hydrolysate.

[0094] Test Example 2: Effect of the high-uric-acid-lowering composition on hyperuricemia in rats

[0095] 2.1 Experimental materials

[0096] 2.1.1 Experimental animals: SD rats, male, 140-160 g, 60 rats, purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd., license number: SCXK (Beijing) 2016-0011.

[0097] 2.2.2 Drugs: The uric acid-lowering composition prepared in Example 1, benzbromarone (Hermann Pharmaceuticals, Germany, batch number: 1513419); adenine (Sigma, batch number: Lot#WXBB0585V); ethambutol (Shenyang Hongqi Pharmaceutical, batch number: 1510121); CMC-Na (Shanghai Zhanyun Chemical, batch number: 170410).

[0098] 2.2 Experimental Methods

[0099] 2.2.1 Grouping and Dosing

[0100] Sixty male rats were randomly divided into six groups: a normal group, a model group, and high-, medium-, and low-dose solid beverage groups, with ten rats in each group. The rats were administered the medication by gavage at the same time every morning. The normal and model groups were administered pure water by gavage, while the other groups were administered the corresponding medication by gavage. (The high-, medium-, and low-dose groups of the hyperuricemic composition prepared in Example 1 contained 4.8 g crude drug / kg, 2.4 g crude drug / kg, and 1.2 g crude drug / kg, respectively, and benzbromarone was administered at a dose of 20 mg / kg, all prepared with pure water.) The gavage volume was 10 ml / kg for each group, and the administration continued for nine days.

[0101] 2.2.2 Establishment of a hyperuricemia model

[0102] Adenine was prepared as a suspension using 0.5% CMC-Na at a dose of 100 mg / kg, and ethambutol was prepared using pure water at a dose of 250 mg / kg. The corresponding drugs were administered by gavage at the same time every afternoon to establish the model. Except for the normal group animals, which were administered 0.5% CMC-Na (10 ml / kg) by gavage, the other animals were administered adenine (5 ml / kg) and ethambutol (5 ml / kg) by gavage. The model was established for 6 consecutive days (the first modeling was started 3 days after the drug administration). One hour after the last modeling, about 1 ml of blood was collected from the retro-orbital venous plexus of all animals. After standing at room temperature for 1 hour, the serum was separated by centrifugation at 3500 r / min for 10 min.

[0103] 2.2.3 Detection Indicators

[0104] Serum uric acid (UA), creatinine (CREA), and blood urea nitrogen (BUN) levels were measured using a fully automated biochemical analyzer.

[0105] After blood collection from the animals, they were quickly euthanized by dislocation of the neck. Kidney tissue was rapidly separated on an ice table. The right kidney was demembranes and medulla were removed, and it was split in half. One half was frozen in liquid nitrogen and stored at -80°C for protein assays. The other half was immediately shredded on the ice table and stored at -80°C for RNA extraction. Simultaneously, the left kidney was dissected, surface fat removed, and soaked in 4% formalin for histopathological examination. The results are shown in Table 2.

[0106] Table 2. Effects of Panax notoginseng solid beverage on rats with hyperuricemia (x±s, n=10)

[0107]

[0108] Note: Compared with the model group, *P<0.05, **P<0.01; compared with the normal group, △P<0.05.

[0109] △△ P < 0.01

[0110] As shown in Table 2, compared with the normal group, the weight of animals in the other groups decreased, and the serum creatinine (CREA) and blood urea nitrogen (BUN) increased, with significant differences (P < 0.01). The serum uric acid (UA) of the model group was significantly higher than that of the normal group (P < 0.01), indicating that the rat model of hyperuricemia with renal excretion disorder was successfully established.

[0111] Compared with the model group, serum uric acid levels decreased in all treatment groups. Except for the low-dose group (1200 mg crude drug / kg), the high- and medium-dose groups (4800 and 2400 mg crude drug / kg) and the benzbromarone group (20 mg / kg) showed significant decreases in serum uric acid levels (P < 0.05). This indicates that the high- and medium-dose groups of the uric acid-lowering composition prepared in Example 1 can significantly reduce serum uric acid levels in animals.

[0112] Compared with the model group, all treatment groups showed a decrease in serum creatinine (CREA) and blood urea nitrogen (BUN) levels, and the high-dose group (4800 mg crude drug / kg, yield 20%) showed a significant decrease in serum creatinine (CREA) and blood urea nitrogen (BUN) levels (P < 0.05 or P < 0.01). This indicates that the high-dose composition for lowering uric acid prepared in Example 1 has a certain protective effect on renal excretion function.

[0113] Finally, it should be noted that the above examples are merely some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments and many variations are possible. All variations that can be directly derived or conceived by those skilled in the art from the disclosure of the present invention should be considered within the scope of protection of the present invention.

Claims

1. A composition for lowering uric acid, characterized in that: It is composed of the following components in parts by weight: 10-25 parts of Panax notoginseng, 10-15 parts of kudzu root, 8-12 parts of stevia, 5-15 parts of Eucommia ulmoides leaves, and 0.1-3 parts of fruit and vegetable powder. The method for preparing the composition for lowering uric acid includes the following steps: After washing and removing impurities from Panax notoginseng, kudzu root, stevia, and eucommia leaves, take the prescribed amount of Panax notoginseng, kudzu root, stevia, and eucommia leaves and pulverize them. Take the pulverized material, add mixed enzyme and purified water to obtain a mixed solution, add pH adjuster to adjust pH to 4.0-5.0, and then perform variable frequency ultrasound-assisted enzymatic extraction at 40-50℃. First, treat with low frequency ultrasound at 30-40 kHz for 10-15 min, and then treat with high frequency ultrasound at 70-80 kHz for 15-20 min; boil for 5-6 min to inactivate the enzyme and obtain the extract. The obtained extract was filtered through 200-300 mesh gauze, and the filtrate was concentrated under reduced pressure to 1 / 3-1 / 2 of the original volume. 3-5 times the volume fraction of 50%-70% ethanol was added to the concentrate for alcohol precipitation, centrifugation, and freeze drying to obtain the compound powder of traditional Chinese medicine extract. A compound powder of traditional Chinese medicine extracts and fruit and vegetable powders are mixed to obtain a composition for lowering high uric acid. The mixed enzyme is cellulase R-10 and dissociative enzyme R-10, wherein the mass ratio of cellulase R-10 to dissociative enzyme R-10 is 2-3:1, and the mixed enzyme accounts for 1%-2% of the total weight of the mixture. The total flavonoid content of the composition for lowering uric acid is on average 10.88 mg / g, and the total flavonoid transfer rate is 89.48%.

2. The composition for lowering uric acid according to claim 1, characterized in that, The fruit and vegetable powder is at least one of prickly pear powder, apple powder, and acerola cherry powder.

3. The method for preparing a composition for lowering uric acid as described in claim 1, characterized in that, The method for preparing a composition for lowering uric acid as described in claim 1, wherein the pH adjuster is at least one selected from citric acid, lactic acid, and malic acid.

4. The method for preparing a composition for lowering uric acid as described in claim 1, characterized in that, The mass ratio of the pulverized material to purified water is 1:6-10.

5. The method for preparing a composition for lowering uric acid as described in claim 1, characterized in that, The centrifugation speed is 4000-6000 rpm, and the centrifugation time is 3-5 minutes.

6. The use of the uric acid-lowering composition as described in claim 1 or 2, or the uric acid-lowering composition prepared by any one of the preparation methods in claims 3-5, in the preparation of a medicament for the prevention and treatment of hyperuricemia.

Images