Sample quantitative transfer device

Abstract

The present application relates to sample quantitative transfer device, belong to sample detection technical field, sample quantitative transfer device includes: sample storage tank, sample storage tank has pressurizing mouth and pressure relief port, liquid in sample storage tank can be pushed to pressure relief port from the pressure of pressurizing mouth;Reaction pool, reaction pool has sample inlet and sample outlet;Switch channel, switch channel is located between sample storage tank and reaction pool, switch channel is provided with first interface that communicates with pressure relief port, second interface that communicates with sample inlet and third interface that communicates with sample outlet;Switch, switch has first connection channel, switch is movably arranged in switch channel, so that switch can reach first position and second position, when switch is in first position, first connection channel communicates first interface with second interface, so as to communicate sample storage tank with reaction pool;When switch is in second position, switch closes second interface and third interface, so as to close reaction pool.

Description

Technical Field

[0001] This invention relates to the field of in vitro diagnostic technology, and in particular to a sample quantitative transfer device. Background Technology

[0002] Sample testing, such as nucleic acid testing, generally includes the following steps: nucleic acid extraction, reaction system preparation, PCR amplification, and quantitative fluorescence detection. Nucleic acid extraction is typically performed inside sample tubes. After extraction, the sample tubes are placed into the reaction system's consumable cartridge. Inside the cartridge, the nucleic acid is transferred from the sample tubes to the reaction chamber, preventing contact between the nucleic acid and external air. During this transfer, the amount of nucleic acid needs to be strictly controlled; too much or too little will hinder subsequent PCR amplification. Therefore, this transfer process is also called quantitative nucleic acid transfer.

[0003] To achieve quantitative transfer of nucleic acids, a piston is usually used to control the flow of nucleic acids, because controlling the movement distance of the piston can more precisely control the amount of nucleic acid flowing.

[0004] The inventors discovered that during the PCR amplification of nucleic acids in the reaction chamber, the reaction chamber and the sample tube are always connected, and the liquid in the reaction chamber can leave the reaction chamber by evaporation, resulting in a reduction in the amount of liquid in the reaction system. Summary of the Invention

[0005] The purpose of this invention is to provide a sample quantitative transfer device that, to a certain extent, avoids the reduction of liquid volume in the reaction system due to liquid evaporation in the reaction tank.

[0006] To achieve the above objectives, embodiments of the present invention provide a sample quantitative transfer device, comprising: A sample storage tank having a pressurization port and a depressurization port, wherein liquid in the sample storage tank can be pushed towards the depressurization port by pressure from the pressurization port; A reaction cell having a sample inlet and a sample outlet; A switching channel is located between the sample storage tank and the reaction cell. The switching channel is provided with a first interface communicating with the pressure relief port, a second interface communicating with the sample inlet, and a third interface communicating with the sample outlet. A switch having a first connection channel, the switch being movably disposed within the switch channel, enabling the switch to reach a first position and a second position. When the switch is in the first position, the first connection channel connects the first interface to the second interface so as to connect the sample storage tank and the reaction tank; When the switch is in the second position, the switch closes the second and third interfaces to shut down the reaction tank.

[0007] In some embodiments, the switch channel is further provided with a drain port, the switch further has a second connection channel, and the sample quantitative transfer device further includes: A liquid collection tank is located on one side of the switch channel and is connected to the drain port; When the switch is in the first position, the second connection channel connects the third interface to the drain port so as to collect the liquid overflowing from the sample outlet in the collection tank.

[0008] In some embodiments, the sample quantitative transfer device further includes: A first liquid channel is connected between the sample storage tank and the switch channel. The first liquid channel has a liquid inlet communicating with the pressure relief port and a liquid outlet communicating with the first interface. Preferably, the pressure relief port is located at the bottom of the sample storage tank.

[0009] In some embodiments, the sample quantitative transfer device further includes: Installation port; A liquid storage tube, wherein the liquid storage tube is detachably disposed in the mounting port; A puncture device is disposed in the installation port and is capable of puncturing the wall of the liquid storage tube; A liquid transfer channel, the inlet of which is located on the puncture device; A displacement channel, wherein the outlet of the liquid transfer channel is located within the displacement channel; A sample quantitative holder, wherein the sample storage slot is disposed on the sample quantitative holder, and the sample quantitative holder is movably disposed within the displacement channel, enabling the sample quantitative holder to reach a third position and a fourth position. When the sample quantification seat is in the third position, the pressurization port is connected to the outlet of the liquid transfer channel; When the sample quantification seat is in the fourth position, the pressurization port is offset from the outlet of the liquid transfer channel.

[0010] In some embodiments, The reservoir tube has a sealable reservoir cavity. When the puncture tool punctures the wall of the reservoir tube, the connection between the puncture tool and the reservoir tube remains sealed, such that the volume of the puncture tool inserted into the reservoir cavity is equal to the volume of liquid discharged from the reservoir cavity; and / or The bottom wall of the reservoir tube is provided with a puncture channel adapted to the puncture device, the inner end of the puncture channel has a fragile area, and there is a height difference between the fragile area and the bottom wall of the reservoir tube; and / or The puncture channel is located at the edge of the bottom wall of the reservoir tube.

[0011] In some embodiments, the sample quantitative transfer device further includes: Piston channel, which is connected to the pressurization port; A piston is disposed within the piston channel and is capable of moving toward the pressurization port to increase the pressure at the pressurization port.

[0012] In some embodiments, the piston has an initial position and a working position, and when the piston moves from the initial position to the working position, the reaction tank is filled with liquid.

[0013] In some embodiments, the piston can be coupled to the switch after moving to the working position, so as to drive the switch to move from the first position to the second position.

[0014] In some embodiments, the sample quantitative transfer device further includes: A connector is disposed on the piston or the switch so that the piston is coupled to the switch via the connector.

[0015] In some embodiments, the sample quantitative transfer device further includes: A connection area that connects the piston channel and the switch channel, wherein the connector passes through the connection area.

[0016] Compared with the prior art, the beneficial effects of the embodiments of the present invention are: a switch channel is provided between the sample storage tank and the reaction cell, and the sample storage tank and the reaction cell can be connected or the reaction cell can be closed by moving the switch in the switch channel. Thus, after the sample in the sample storage tank is quantitatively transferred to the reaction cell, the reaction cell can be closed to prevent the liquid in the reaction cell from leaving the reaction cell by evaporation, so that the amount of liquid in the reaction cell remains basically unchanged. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the sample quantitative transfer device provided in Embodiment 1 of the present invention.

[0018] Figure 2 An exploded view of the sample quantitative transfer device provided in Embodiment 1 of the present invention from one perspective.

[0019] Figure 3 An exploded view of the sample quantitative transfer device provided in Embodiment 1 of the present invention from another perspective.

[0020] Figure 4 This is a schematic diagram of the bottom of the liquid storage tube provided in Embodiment 1 of the present invention.

[0021] Figure 5 for Figure 4 Cross-sectional view at point AA.

[0022] Figure 6 This is a schematic diagram of the fragile area inside the liquid storage tube after damage, as provided in Embodiment 1 of the present invention.

[0023] Figure 7 This is a schematic diagram of the swab head being disposed inside the liquid storage tube according to Embodiment 1 of the present invention.

[0024] Figure 8 This is a schematic diagram of the puncture device provided in Embodiment 1 of the present invention.

[0025] Figure 9 for Figure 8 Cross-sectional view at point BB.

[0026] Figure 10 This is a schematic diagram showing the puncture device provided in Embodiment 1 of the present invention before and after puncturing the vulnerable area.

[0027] Figure 11 This is a schematic diagram of the upper part of the base provided in Embodiment 1 of the present invention.

[0028] Figure 12 This is a schematic diagram of the bottom of the base provided in Embodiment 1 of the present invention.

[0029] Figure 13 for Figure 11 Cross-sectional view at point CC.

[0030] Figure 14 This is a schematic diagram of the upper part of the sample quantification seat provided in Embodiment 1 of the present invention.

[0031] Figure 15 This is a schematic diagram of the bottom of the sample quantification seat provided in Embodiment 1 of the present invention.

[0032] Figure 16 This is a top view of the base provided in Embodiment 1 of the present invention, wherein the sample quantification seat is located in the third position.

[0033] Figure 17 for Figure 16 Cross-sectional view at point DD.

[0034] Figure 18 This is a top view of the base provided in Embodiment 1 of the present invention, wherein the sample quantification seat is located in the fourth position.

[0035] Figure 19 for Figure 18 Cross-sectional view at EE.

[0036] Figure 20This is a schematic diagram of the top of the cover provided in Embodiment 1 of the present invention.

[0037] Figure 21 This is a schematic diagram of the bottom of the upper cover provided in Embodiment 1 of the present invention.

[0038] Figure 22 This is a schematic diagram of the switch provided in Embodiment 1 of the present invention.

[0039] Figure 23 This is a top view of the sample quantitative transfer device provided in Embodiment 1 of the present invention.

[0040] Figure 24 for Figure 23 A cross-sectional view at point FF, where the switch is in its initial position.

[0041] Figure 25 for Figure 23 A cross-sectional view at point FF, where the switch is in the first position.

[0042] Figure 26 for Figure 23 A cross-sectional view at point FF, where the switch is in the second position.

[0043] Figure 27 This is a cross-sectional view of the sample quantitative transfer device provided in Embodiment 2 of the present invention.

[0044] In the picture: 100. Outer shell; 110. Base; 111. Mounting port; 1111. Mounting groove; 112. Second liquid channel; 1121. Channel inlet; 1122. Channel outlet; 113. Gas channel; 1131. Gas inlet; 1132. Gas outlet; 114. First liquid channel; 1141. Liquid inlet; 1142. Liquid outlet; 115. Shifting channel; 120. Top cover; 121. Piston channel; 122. Switch channel; 1222. Second interface; 1223. Third interface; 1224. Drain port; 124. Connection port; 130. End cap; 200. Liquid reservoir tube; 210. Tube body; 211. Bottom wall; 212. Side wall; 220. Tube cap; 230. Liquid reservoir cavity; 240. Puncture channel; 250. Vulnerable area; 260. Swab head; 300. Puncture element; 310. Puncture section; 311. Puncture head; 320. Mounting section; 330. Third fluid channel; 400. Sample quantification stand; 410. Sample storage tank; 420. Pressurization port; 430. Pressure relief port; 440. Liquid collection tank; 500, Piston; 600, switch; 610, first connection channel; 620, second connection channel; 700. Reaction vessel; 710. Reaction cell; 711. Sample inlet; 712. Sample outlet; 800. Connectors. Detailed Implementation

[0045] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, only the parts relevant to the present invention are shown in the accompanying drawings, not all of them.

[0046] Example 1 Figure 1 A schematic diagram of the sample quantification device provided in this embodiment is shown. Figure 2 An exploded view of the sample quantification device provided in this embodiment is shown. Figure 1 and Figure 2 As shown, the sample quantification device includes a housing 100, a liquid storage tube 200, a piston 500, and a reaction vessel 700. The housing 100 has a mounting port 111 for installing the liquid storage tube 200, which can be longitudinally held within the mounting port 111. In this embodiment, the liquid storage tube 200 is detachably disposed in the mounting port 111. For example, when a sample needs to be collected, the liquid storage tube 200 can be removed from the mounting port 111; after the liquid storage tube 200 extracts the sample, it can be reinstalled into the mounting port 111. When the liquid storage tube 200 is installed in the mounting port 111, the tube wall is punctured, allowing the liquid storage tube 200 to communicate with the reaction vessel 700. Then, by moving the piston 500, the liquid in the liquid storage tube 200 can be transferred to the reaction pool 710 of the reaction vessel 700, and typically, after the liquid transfer, the reaction pool 710 is filled with liquid.

[0047] Figure 3 An exploded view of another perspective of the sample quantification device provided in this embodiment is shown.

[0048] like Figure 2 and Figure 3 As shown, an upward-protruding piercing element 300 is provided inside the installation port 111. When the liquid storage tube 200 is installed into the installation port 111, the bottom of the liquid storage tube 200 and the piercing element 300 are pressed against each other, so that the piercing element 300 pierces the bottom of the liquid storage tube 200. A third liquid channel 330 is provided on the piercing element 300. After the piercing element 300 pierces the bottom of the liquid storage tube 200, one end of the third liquid channel 330 extends into the interior of the liquid storage tube 200, thereby transferring the liquid inside the liquid storage tube 200 to the outside of the liquid storage tube 200 so that the liquid can be transferred to the reaction vessel 700 later.

[0049] like Figure 2 and Figure 3 As shown, the outer casing 100 includes a base 110 and a top cover 120. The puncture device 300 is mounted on the base 110. A sample quantification seat 400 is also provided inside the base 110. A sample storage tank 410 is provided on the sample quantification seat 400. The sample storage tank 410 is connected to a second liquid channel 112 provided on the base 110 (see...). Figure 12 This is connected to the third liquid channel 330 mentioned above, so that the liquid in the liquid storage tube 200 can flow into the sample storage tank 410, and a predetermined amount is achieved in the sample storage tank 410. For example... Figure 3 As shown, in this embodiment, the base 110 is provided with a laterally extending displacement channel 115, and the sample quantitative seat 400 is movably disposed within the displacement channel 115. The sample quantitative seat 400 is provided with a pressure port 420 communicating with the sample storage tank 410 (see...). Figure 14 When the sample metering seat 400 moves in the shifting channel 115, the position of the pressure port 420 moves accordingly. Specifically, when the pressure port 420 is connected to the second liquid channel 112, the liquid in the storage tube 200 can enter the sample storage tank 410; otherwise, it cannot enter the sample storage tank 410. After a predetermined amount of liquid has been collected in the sample storage tank 410, the sample metering seat 400 can be moved to offset the pressure port 420 from the second liquid channel 112, so that a predetermined amount of liquid is maintained in the sample storage tank 410. In addition, in this embodiment, the top of the sample storage tank 410 is an upward-opening pressure relief port 430.

[0050] like Figure 2 and Figure 3 As shown, the outer casing 100 is also provided with a gas channel 113 and a first liquid channel 114 (see...). Figure 24 ). Figure 3 The diagram shows a portion of a gas channel 113 and a first liquid channel 114. The gas inlet of the gas channel 113 is connected to the upper cover 120, and the gas outlet of the gas channel 113 is located in the shift channel 115. When the piston 500 moves in the piston channel 121 in the upper cover 120, the gas in the gas channel 113 is pushed by the piston 500 and flows toward the gas outlet. If the pressurization port 420 on the sample metering seat 400 is connected to the gas outlet at this time, the liquid in the sample storage tank 410 is pushed upward and enters the first liquid channel 114 from the pressure relief port 430.

[0051] like Figure 2 and Figure 3As shown, a connection port 124 for installing the reaction container 700 is provided on one side of the top cover 120. The connection port 124 is connected to the first liquid channel 114 so that the liquid entering the first liquid channel 114 can flow into the connection port 124 and then enter the reaction pool 710 inside the reaction container 700 through the sample inlet 711 on the reaction container 700. It should be noted that the amount of liquid in the sample storage tank 410 is sufficient to fill the reaction pool 710.

[0052] like Figure 3 As shown, the outer casing 100 also includes an end cap 130. After the reaction container 700 is installed on the connection port 124, the end cap 130 passes through the reaction container 700 and connects to the upper cover 120 and the base 110, thereby fixing the reaction container 700 to the outer casing 100. In addition, the end cap 130 also confines the sample quantification seat 400 within the displacement channel 115, preventing the sample quantification seat 400 from falling out of the displacement channel 115.

[0053] like Figure 2 and Figure 3 As shown, the upper cover 120 is also provided with a piston channel 121 and a switch channel 122 extending longitudinally downward from the top surface. The piston 500 is movably disposed in the piston channel 121, and the switch 600 is movably disposed in the switch channel 122. The lower end of the piston channel 121 is connected to the gas channel 113. When the piston 500 moves in the piston channel 121, the gas in the gas channel 113 is compressed, thereby moving towards the displacement channel 115. If the pressure port 420 of the sample metering seat 400 is connected to the gas channel 113 at this time, the liquid in the sample storage tank 410 moves towards the pressure relief port 430 under pressure. The switch channel 122 connects the first liquid channel 114 and the reaction tank 710. When the switch 600 moves in the switch channel 122, it can connect or disconnect the first liquid channel 114 from the reaction tank 710.

[0054] The specific structure of the sample quantitative transfer device will be described below in the order of liquid flowing from the storage tube 200 to the reaction cell 710.

[0055] Figure 4 A schematic diagram of the bottom of the liquid storage tube 200 provided in this embodiment is shown. Figure 4As shown, the liquid storage tube 200 includes a tube body 210 and a cap 220 connected to the top of the tube body 210. The tube body 210 is a hollow tube, and a longitudinally extending liquid storage cavity 230 is provided inside the tube body 210. An opening is formed at the top of the liquid storage cavity 230, and the cap 220 is closed over the opening to seal the liquid storage cavity 230. The tube body 210 includes a bottom wall 211 and a side wall 212 connected to the bottom wall 211. The bottom wall 211 defines the bottom of the liquid storage cavity 230, and the side wall 212 defines the side of the liquid storage cavity 230. A puncture channel 240 extending toward the liquid storage cavity 230 is provided on the bottom wall 211. A fragile area 250 is provided within the puncture channel 240, separating the puncture channel 240 from the liquid storage cavity 230. The fragile area 250 refers to the area that can be damaged when subjected to a certain pressure. Commonly, the fragility function can be achieved by reducing the wall thickness of the fragility zone 250. Of course, it is also feasible to use a less strong material to manufacture the fragility zone 250.

[0056] Figure 5 It shows Figure 4 Cross-sectional view at point AA. (See diagram below.) Figure 5 As shown, a height difference is formed between the fragile region 250 and the bottom wall 211, dividing the reservoir 230 into an upper chamber higher than the fragile region 250 and a lower chamber lower than the fragile region 250. The purpose of setting the fragile region 250 higher than the bottom wall 211 is to ensure that denser impurities and residues in the sample are contained in the lower chamber. Even if the fragile region 250 is punctured, these substances will remain in the lower chamber and will not flow out of the reservoir 200, thus avoiding interference with the sample quantification process.

[0057] Figure 6 This diagram illustrates the situation after the vulnerable area 250 in this embodiment has been punctured. (See diagram below.) Figure 6 As shown, when the vulnerable area 250 is damaged, the puncture channel 240 and the liquid storage chamber 230 are connected to each other at the original position of the vulnerable area 250 so that the liquid in the upper chamber flows into the puncture channel 240.

[0058] Figure 7 A cross-sectional view of the reservoir tube 200 in this embodiment is shown, and the swab head 260 is disposed inside the reservoir tube 200. Figure 7 As shown, in this embodiment, the puncture channel 240 is located at the edge of the bottom wall 211, giving the lower chamber a relatively large accommodating space so that the swab head 260 can still be placed inside the tube body 210 without increasing the diameter and / or length of the tube body 210. However, it is not excluded that the puncture channel 240 may be far from the edge of the bottom wall 211.

[0059] Figure 8 A perspective view of the puncture member 300 provided in this embodiment is shown. Figure 8 As shown, the puncture member 300 includes a puncture part 310 and a mounting part 320. The puncture part 310 can extend into the puncture channel 240, and the mounting part 320 can be installed in the mounting port 111. The top of the puncture part 310 is a puncture head 311, which can puncture the vulnerable area 250. The puncture member 300 also has a third liquid channel 330. The inlet of the third liquid channel 330 is located on the puncture head 311, and the outlet of the third liquid channel 330 is located on the mounting part 320. When the puncture head 311 punctures the vulnerable area 250, the liquid storage chamber 230 communicates with the third liquid channel 330, so that liquid enters the second liquid channel 112 on the base 110 through the third liquid channel 330.

[0060] Figure 9 It shows Figure 8 Cross-sectional view at point BB. (See diagram below.) Figure 9 As shown, in this embodiment, the outlet of the third liquid channel 330 is located at the bottom of the mounting portion 320 so as to facilitate communication with the second liquid channel 112 located at the bottom of the mounting port 111.

[0061] Furthermore, the puncture member 300 has an annular groove in its center, within which a sealing ring (not shown) is placed. When the puncture part 310 extends into the puncture channel 240, the sealing ring seals the connection between the puncture channel 240 and the puncture part 310. This prevents outside air from contacting the liquid even if the vulnerable area 250 is punctured. Simultaneously, this ensures that the volume of the puncture member 300 extending into the liquid storage cavity 230 is equal to the volume of liquid flowing out of the liquid storage cavity 230. Of course, in other embodiments, a sealing ring may not be used; instead, the puncture part 310 and the puncture channel 240 may be press-fitted together to achieve a seal.

[0062] Figure 10 This illustration shows a schematic diagram before and after the puncture device 300 provided in this embodiment punctures the vulnerable area 250. (See diagram below.) Figure 10 As described above, when it is necessary to puncture the vulnerable area 250, the reservoir cavity 230 of the reservoir tube 200 is kept sealed, the puncture member 300 is aligned with the puncture channel 240, and then the puncture member 300 and the reservoir tube 200 are brought close to each other, so that the puncture part 310 extends into the puncture channel 240, and continues to move closer to the puncture member 300 and the reservoir tube 200, so that the puncture head 311 abuts against the vulnerable area 250. As the puncture head 311 applies greater force to the vulnerable area 250, the vulnerable area 250 is eventually broken, so that the puncture head 311 extends into the reservoir cavity 230.

[0063] Figure 11 A schematic diagram of the upper part of the base 110 provided in this embodiment is shown. (See diagram below.) Figure 11As shown, a mounting groove 1111 is provided on the base 110 at a position corresponding to the mounting opening 111. The shape of the mounting groove 1111 is adapted to the shape of the mounting portion 320 on the piercing member 300, so that the piercing member 300 can be placed in the mounting groove 1111. It should be noted that in other embodiments, the piercing member 300 may be fixed on the base 110 and cannot be separated from the base 110, for example, the piercing member 300 and the base 110 may be integrally formed.

[0064] like Figure 11 As shown, the inlet 1121 of the second liquid channel 112 is located in the mounting groove 1111, and the outlet 1122 of the second liquid channel 112 is located in the displacement channel 115 and on the bottom wall of the displacement channel 115.

[0065] It should be noted that, in this embodiment, when the second liquid channel 112 is connected to the third liquid channel 330, the second liquid channel 112 and the third liquid channel 330 can be referred to as liquid transfer channels.

[0066] Figure 12 A schematic diagram of the bottom of the base 110 provided in this embodiment is shown. Figure 12 The arrows indicate the flow direction of the liquid in the second liquid channel 112, or the flow direction of the gas in the gas channel 113. For example... Figure 12 As shown, the second liquid channel 112 extends from the bottom of the mounting port 111 to the bottom of the shift channel 115, so that liquid enters the channel outlet 1122 from the channel inlet 1121. Furthermore, a portion of the gas channel 113 is located at the bottom of the base 110, and the gas outlet 1132 of the gas channel 113 is located at the bottom of the shift channel 115. Like the channel outlet 1122, the gas outlet 1132 is also located on the bottom wall of the shift channel 115. Thus, when the sample metering seat 400 moves within the shift channel 115, the pressurization port 420 can selectively communicate with either the channel outlet 1122 or the gas outlet 1132.

[0067] Figure 13 It shows Figure 11 Cross-sectional view at point CC. Figure 13 The arrows in the diagram indicate the direction of gas or liquid flow. For example... Figure 13As shown, in this embodiment, the gas channel 113 is approximately L-shaped. The gas inlet 1131 of the gas channel 113 is connected to the upper cover 120, and the gas outlet 1132 of the gas channel 113 extends upward from the bottom of the base 110 to the bottom of the shift channel 115. The liquid inlet 1141 of the first liquid channel 114 is located at the top of the shift channel 115 and is directly opposite the gas outlet 1132. The upper end of the first liquid channel 114 extends into the upper cover 120. When the pressurization port 420 of the sample metering seat 400 is connected to the gas outlet 1132, as the gas in the gas channel 113 is compressed by the piston 500, the pressure at the gas outlet 1132 is greater than that at the liquid inlet 1141. Therefore, the liquid in the sample storage tank 410 enters the liquid inlet 1141 under the action of the pressure difference.

[0068] Figure 14 A schematic diagram of the upper part of the sample quantification seat 400 provided in this embodiment is shown. Figure 15 A schematic diagram of the bottom of the sample quantification holder 400 provided in this embodiment is shown. Figure 14 As shown, a sample storage tank 410 is provided on the upper surface of the sample quantification holder 400, and a pressure port 420 is provided at the bottom of the sample storage tank 410, such as... Figure 15 As shown, the pressurization port 420 faces the bottom surface of the sample metering seat 400. Therefore, when the sample metering seat 400 moves in the shifting channel 115, the pressurization port 420 faces the bottom surface of the shifting channel 115. Consequently, when the sample metering seat 400 moves to a suitable position in the shifting channel 115, it can communicate with the gas outlet 1132 or the channel outlet 1122. Since the pressurization port 420 is located at the bottom of the sample storage tank 410, liquid can only enter from the bottom of the sample storage tank 410, rather than dripping into the sample storage tank 410, thus preventing liquid loss due to splashing.

[0069] like Figure 14 As shown, the upper end of the sample storage tank 410 is the pressure relief port 430, which faces the top surface of the shift channel 115. Therefore, the pressure relief port 430 can communicate with the liquid inlet 1141. In particular, when the pressurization port 420 is connected to the gas inlet 1131, the pressure relief port 430 is connected to the liquid inlet 1141. Furthermore, as... Figure 14As shown, a liquid collection tank 440 is also provided on the upper surface of the sample quantitative holder 400. The liquid collection tank 440 is located on one side of the sample storage tank 410 and is used to collect liquid overflowing from the sample storage tank 410. This ensures that the sample storage tank 410 is exactly filled with liquid to achieve the predetermined amount of liquid. In this embodiment, the liquid collection tank 440 is located near the mounting port 111, or in other words, away from the reaction vessel 700, so that when the liquid in the sample storage tank 410 is transferred to the reaction vessel 700, the liquid collection tank 440 can receive the liquid overflowing from the reaction vessel 700. The specific overflow process will be described in detail later.

[0070] Figure 16 A top view of the base 110 and sample quantitative seat 400 provided in this embodiment is shown, wherein the sample quantitative seat 400 is in the third position. Figure 17 It shows Figure 16 Cross-sectional view at point DD. (See diagram below.) Figure 17 As shown, when the sample metering seat 400 is in the third position, the pressure port 420 is connected to the channel outlet 1122 so that the liquid in the liquid storage chamber 230 can enter the sample storage tank 410. And when the sample storage tank 410 is full of liquid, the excess liquid can overflow from the pressure relief port 430, so that the excess liquid can flow into the collection tank 440.

[0071] Figure 18 A top view of the base 110 and sample quantitative seat 400 provided in this embodiment is shown, wherein the sample quantitative seat 400 is in the fourth position. Figure 19 It shows Figure 18 Cross-sectional view at the EE section. (See diagram below.) Figure 19 As shown, when the sample quantification seat 400 is in the fourth position, the pressurization port 420 is no longer connected to the channel outlet 1122, but is connected to the gas outlet 1132, and the pressure relief port 430 is connected to the liquid inlet 1141, so that under the action of air pressure, the liquid in the sample storage tank 410 can enter the first liquid channel 114.

[0072] Figure 20 A schematic diagram of the top of the cover 120 provided in this embodiment is shown. Figure 20As shown, a piston channel 121 and a switch channel 122 are provided on the top surface of the upper cover 120. The piston 500 is movably disposed in the piston channel 121, and the switch 600 is movably disposed in the switch channel 122. The lower end of the piston channel 121 is connected to the gas inlet 1131, so that when the piston 500 moves in the piston channel 121, the gas pressure in the gas channel 113 changes. The switch channel 122 is connected to the first liquid channel 114 and also to the reaction vessel 700. When the switch 600 moves in the switch channel 122, it can control the opening and closing of the connection between the first liquid channel 114 and the reaction vessel 700.

[0073] like Figure 20 As shown, in this embodiment, the piston channel 121 and the switch channel 122 are interconnected, allowing the connector 800 connected to the piston 500 to extend into the switch channel 122. Therefore, when the piston 500 moves within the piston channel 121, it can drive the switch 600 to move synchronously. Specifically, the piston 500 and the switch 600 can always be connected via the connector 800, or the connector 800 can only control the movement of the switch 600 at certain positions. For example, the connector 800 will only contact the top surface of the switch 600 after the piston 500 has pressed down a certain distance, thereby pushing the switch 600 to move. More specifically, the connector 800 can be configured to push the switch 600 from the first position to the second position, as described below.

[0074] Of course, in other embodiments, the connector 800 may also be disposed on the piston 500.

[0075] Figure 21 A schematic diagram of the bottom of the upper cover 120 provided in this embodiment is shown. (See diagram below.) Figure 21 As shown, the switch channel 122 is located between the first liquid channel 114 and the connection port 124, so that the switch 600 located in the switch channel 122 can control the on / off connection between the first liquid channel 114 and the connection port 124, that is, control the on / off connection between the first liquid channel 114 and the reaction vessel 700.

[0076] like Figure 21 As shown, a first pair of interfaces is provided on the wall of switch channel 122 (see...). Figure 24 The system includes a second pair of interfaces, a third pair of interfaces, and a drain outlet 1224. The first pair of interfaces is connected to the liquid outlet 1142, or it can be considered that the first pair of interfaces is the liquid outlet 1142. The second pair of interfaces is connected to the sample inlet 711, the third pair of interfaces is connected to the sample outlet 712, and the drain outlet 1224 is connected to the collection tank 440. Figure 22As shown, the switch 600 is provided with a first connection channel 610 and a second connection channel 620. When the switch 600 is in the first position, the first connection channel 610 can connect the first pair of interfaces and the second pair of interfaces, and the second connection channel 620 can connect the third pair of interfaces and the drain port 1224.

[0077] When switch 600 is in the first position, liquid enters the first pair of interfaces from the liquid outlet 1142 of the first liquid channel 114, then enters the second pair of interfaces through the first connecting channel 610, and then enters the reaction tank 710. When the reaction tank 710 is full of liquid, the liquid overflowing from the reaction tank 710 enters the third pair of interfaces, and enters the drain port 1224 through the second connecting channel 620, and finally enters the collection tank 440. Thus, it is possible to observe whether there is liquid dripping in the collection tank 440 to determine whether the reaction tank 710 is full. For easy observation, the top surface of the cover 120 can be transparent.

[0078] Figure 23 A top view of the sample quantitative transfer device provided in this embodiment is shown. Figure 24 It shows Figure 23 A cross-sectional view at point FF, where switch 600 is in its initial position. Figure 24 It shows Figure 23 A cross-sectional view at point FF, where switch 600 is in the first position. Figure 24 It shows Figure 23 A cross-sectional view at point FF, where switch 600 is in the second position.

[0079] like Figure 24 As shown, when switch 600 is in the initial position, the first pair of interfaces and the second pair of interfaces are separated by switch 600, and the third pair of interfaces and the drain port 1224 are also separated by switch 600. At this time, the liquid is held in the sample storage tank 410.

[0080] like Figure 25 As shown, as piston 500 moves downward, switch 600 is correspondingly driven and also moves downward. When switch 600 moves to the first position, the first connecting channel 610 connects the first pair of interfaces and the second pair of interfaces, and the second connecting channel 620 connects the third pair of interfaces and the drain port 1224. Thus, liquid in sample storage tank 410 can enter reaction tank 710 along the first liquid channel 114 and the first connecting channel 610, and liquid in reaction tank 710 can enter collection tank 440 from drain port 1224 along the second connecting channel 620.

[0081] like Figure 26As shown, as piston 500 moves further downward, switch 600 is further driven by piston 500, causing switch 600 to leave the first position and enter the second position. At this time, the first pair of interfaces and the second pair of interfaces are separated by switch 600, and the third pair of interfaces and the drain port 1224 are separated by switch 600. Thus, both sample inlet 711 and sample outlet 712 are closed by switch 600, and the liquid in reaction chamber 710 is kept in reaction chamber 710 and cannot leave reaction chamber 710. Therefore, during PCR amplification, the liquid in reaction chamber 710 cannot leave reaction chamber 710 by evaporation.

[0082] In this embodiment, the piston 500 has an initial position and a working position. As the piston 500 moves from the initial position to the working position, liquid gradually enters the reaction tank 710 from the sample storage tank 410. When the piston 500 is in the working position, the reaction tank 710 is filled, which can be observed as dripping at the drain port 1224. When the piston 500 continues to move downwards from the working position, the piston 500 drives the switch 600 to leave the first position.

[0083] The process of using the sample quantitative transfer device provided in this embodiment is as follows: S1 - Nucleic Acid Pre-quantification Process: The reservoir tube 200, with its reservoir chamber 230 sealed, is placed into the mounting port 111, and the puncture channel 240 of the reservoir tube 200 is aligned with the puncture element 300 in the mounting port 111. The reservoir tube 200 is then moved toward the puncture element 300, causing the puncture head 311 at the top of the puncture element 300 to abut against the vulnerable area 250 within the puncture channel 240. As the reservoir tube 200 is further pressed down, the puncture head 311 punctures the vulnerable area 250 and extends into the reservoir chamber 230, thereby storing the nucleic acid. The liquid in the liquid chamber 230 can leave the liquid storage chamber 230 along the third liquid channel 330 on the puncture head 311, and then the liquid enters the second liquid channel 112, and finally enters the shift channel 115 from the channel outlet 1122. It should be noted that at this time the sample quantitative seat 400 is in the third position, so the pressure port 420 is connected to the channel outlet 1122, so that the liquid can flow upward into the sample storage tank 410 until the liquid overflows from the sample storage tank 410, indicating that the sample storage tank 410 has collected a predetermined amount of liquid.

[0084] S2 - Nucleic Acid Quantitative Transfer Process: The sample quantification seat 400, which is in the third position, is moved to the fourth position, so that the pressurization port 420 is connected to the gas outlet 1132 of the gas channel 113. At the same time, the pressure relief port 430 is connected to the liquid inlet 1141 of the first liquid channel 114. Then, the piston 500 is moved downward, so that the gas in the gas channel 113 flows from the gas inlet 1131 to the gas outlet 1132, which increases the gas pressure at the gas outlet 1132. Under the action of pressure, the liquid in the sample storage tank 410 flows upward into the liquid inlet 1141. As the piston 500 continues to move downward, the liquid flows out from the liquid outlet 1142 and enters the sample inlet 711 through the first connecting channel 610, thereby entering the reaction tank 710. When the reaction tank 710 is filled with liquid, the overflowing liquid is discharged from the sample outlet 712, and the discharged liquid enters the drain port 1224 through the second connecting channel 620 and finally drips into the collection tank 440.

[0085] S3 - Reaction Pool Closure Process: After the reaction pool 710 is filled with liquid, the piston 500 drives the switch 600 downward through the connector 800, causing the switch 600 to leave the first position. This changes the position of the first connecting channel 610 and the second connecting channel 620, not only disconnecting the first interface from the second interface 1222 and the third interface 1223 from the drain port 1224, but also causing the switch 600 to close the second interface 1222 and the third interface 1223, thereby sealing the reaction pool 710 and preventing the liquid in the reaction pool 710 from leaving the reaction pool 710.

[0086] Example 2 Unlike the technical solution in Embodiment 1 where the pressure port 420 is located at the bottom of the sample storage tank 410 and the pressure relief port 430 is located at the top of the sample storage tank 410, with the liquid leaving the sample storage tank 410 from bottom to top, this embodiment provides a technical solution where the pressure port 420 is located at the top of the sample storage tank 410 and the pressure relief port 430 is located at the bottom of the sample storage tank 410, with the liquid leaving the sample storage tank 410 from top to bottom.

[0087] Compared to the technical solution of Embodiment 1, the technical solution of Embodiment 2 can reduce the problem of air bubbles generated during liquid transfer to a certain extent, making the liquid volume in the reaction tank 710 more accurate.

[0088] Figure 27 A cross-sectional view of the sample quantitative transfer device provided in Embodiment 2 is shown, as follows. Figure 27 As shown, when the sample metering seat 400 is in the fourth position, the piston channel 121 and the gas channel 113 are located above the sample storage tank 410, the pressurization port 420 is connected to the gas outlet 1132, and the pressure relief port 430 is connected to the liquid inlet 1141 of the first liquid channel 114.

[0089] When the piston 500 moves downward in the piston channel 121, the gas in the gas channel 113 flows toward the gas outlet 1132, thereby increasing the gas pressure at the pressurization port 420, creating a pressure difference between the pressurization port 420 and the pressure relief port 430. Under the action of the pressure difference, the liquid in the sample storage tank 410 flows out from the pressure relief port 430, and then the liquid flows from the liquid inlet 1141 into the first liquid channel 114, and flows along the first liquid channel 114 into the liquid outlet 1142. After that, the flow path of the liquid is the same as in Example 1.

[0090] The foregoing description of embodiments of the present invention, through which those skilled in the art are able to implement or use the present invention, will be readily apparent to those skilled in the art. Various modifications to these embodiments will be readily apparent to those skilled in the art. The general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novelty disclosed herein.

Claims

1. A sample quantitative transfer device, comprising: A sample storage tank (410) has a pressurization port (420) and a pressure relief port (430), wherein liquid in the sample storage tank (410) can be pushed towards the pressure relief port (430) by pressure from the pressurization port (420). The reaction cell (710) has a sample inlet (711) and a sample outlet (712). A switch channel (122) is located between the sample storage tank (410) and the reaction tank (710). The switch channel (122) is provided with a first interface communicating with the pressure relief port (430), a second interface (1222) communicating with the sample inlet (711), and a third interface (1223) communicating with the sample outlet (712). A switch (600) having a first connection channel (610) is movably disposed within the switch channel (122), allowing the switch (600) to reach a first position and a second position. When the switch (600) is in the first position, the first connection channel (610) connects the first interface to the second interface (1222) so as to connect the sample storage tank (410) and the reaction tank (710). When the switch (600) is in the second position, the switch (600) closes the second interface (1222) and the third interface (1223) in order to close the reaction tank (710). The sample quantitative transfer device further includes: Installation port (111); A liquid storage tube (200) is detachably disposed in the mounting port (111); A puncture element (300) is disposed in the mounting port (111) and is capable of puncturing the wall of the liquid storage tube (200); A liquid transfer channel, the inlet of which is located on the puncture member (300); The outlet of the liquid transfer channel is located within the displacement channel (115); A sample quantitative holder (400) is provided, and a sample storage slot (410) is disposed on the sample quantitative holder (400). The sample quantitative holder (400) is movably disposed within the shift channel (115) so that the sample quantitative holder (400) can reach the third and fourth positions. When the sample quantification seat (400) is in the third position, the pressurization port (420) is connected to the outlet of the liquid transfer channel; When the sample quantification seat (400) is in the fourth position, the pressurization port (420) is offset from the outlet of the liquid transfer channel. The liquid storage tube (200) has a sealable liquid storage cavity (230). When the puncture member (300) punctures the wall of the liquid storage tube (200), the connection between the puncture member (300) and the liquid storage tube (200) remains sealed so that the volume of the puncture member (300) inserted into the liquid storage cavity (230) is equal to the volume of liquid discharged from the liquid storage cavity (230). The bottom wall (211) of the liquid storage tube (200) is provided with a puncture channel (240) adapted to the puncture member (300). The puncture channel (240) extends toward the liquid storage cavity (230). The inner end of the puncture channel (240) has a ruptureable area (250). There is a height difference between the ruptureable area (250) and the bottom wall (211) of the liquid storage tube (200), so that the liquid storage cavity (230) is divided into an upper cavity with a height higher than the ruptureable area (250) and a lower cavity with a height lower than the ruptureable area (250). The puncture channel (240) is located at the edge of the bottom wall (211) of the reservoir tube (200).

2. The sample quantitative transfer device according to claim 1, wherein, The switch channel (122) is also provided with a drain port (1224), the switch (600) also has a second connection channel (620), and the sample quantitative transfer device further includes: A liquid collection tank is located on one side of the switch channel (122) and is connected to the drain port (1224); When the switch (600) is in the first position, the second connection channel (620) connects the third interface (1223) to the drain port (1224) so ​​as to collect the liquid overflowing from the sample outlet (712) in the collection tank.

3. The sample quantitative transfer device according to claim 1, wherein, The sample quantitative transfer device further includes: A first liquid channel (114) is connected between the sample storage tank (410) and the switch channel (122). The first liquid channel (114) has a liquid inlet (1141) communicating with the pressure relief port (430) and a liquid outlet (1142) communicating with the first interface.

4. The sample quantitative transfer device according to claim 3, wherein, The pressure relief port (430) is located at the bottom of the sample storage tank (410).

5. The sample quantitative transfer device according to any one of claims 1 to 4, wherein, The sample quantitative transfer device further includes: Piston passage (121), the piston passage (121) is connected to the pressurization port (420); A piston (500) is disposed in the piston channel (121) and is capable of moving toward the pressurization port (420) to increase the pressure at the pressurization port (420).

6. The sample quantitative transfer device according to claim 5, wherein, The piston (500) has an initial position and a working position, and when the piston (500) moves from the initial position to the working position, the reaction tank (710) is filled with liquid.

7. The sample quantitative transfer device according to claim 6, wherein, After the piston (500) moves to the working position, it can couple with the switch (600) so as to drive the switch (600) to move from the first position to the second position.

8. The sample quantitative transfer device according to claim 7, wherein, The sample quantitative transfer device further includes: A connector (800) is disposed on the piston (500) or the switch (600) so that the piston (500) is coupled to the switch (600) via the connector (800).

9. The sample quantitative transfer device according to claim 8, wherein, The sample quantitative transfer device further includes: A connection area that connects the piston channel (121) and the switch channel (122), wherein the connector (800) passes through the connection area.

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