Rosin extract composition, method of preparation and use

Abstract

The present application relates to the technical field of medical preparation, and discloses a Russian olive leaf extract composition, a preparation method and application. The composition comprises the following components in parts by weight: 10-20 parts of Russian olive leaf extract derivative, 10-15 parts of bitter gourd extract, 10-15 parts of pueraria extract, 5-10 parts of hawthorn extract and 5-10 parts of astragalus extract. The present application also provides a preparation method. Compared with the prior art, the Russian olive leaf extract composition prepared by the present application has the effects of reducing blood sugar, blood lipid and blood pressure, and is stable in drug efficacy, mild and safe.

Description

Russian leaf extract composition, preparation method and application Technical Field

[0001] This invention relates to the field of medical preparations technology, and in particular to a Russian leaf extract composition, its preparation method, and its application. Background Technology

[0002] Russian-colored leaves are the leaves of two plants in the Rosaceae family: *Malus toringoides* (Rehd.) Hughes. and *Malus tiansitoria* (Batal.) Schneid. In Tibetan areas such as Tibet, Qinghai, and Sichuan, they are used as a health drink, steeped in water as a tea substitute. They are believed to have effects such as relieving indigestion, removing stagnation, and clearing away greasiness, and are used to treat indigestion, hyperglycemia, hyperlipidemia, and hypertension. Currently, more than 20 compounds have been isolated and identified from Russian-colored leaves, mainly including flavonoids, phenols, and aromatic compounds. The flavonoid components of Russian-colored leaves (such as phlorizin and phloretin) possess various important biological activities. Their total flavonoid content is 2.81 times that of leaves of other plants in the same family, such as *Malus spectabilis*; their phlorizin content is approximately 6 times that of leaves of *Malus hupehensis* and 33 times that of leaves of *Malus tiansitoria*.

[0003] The number of people with diabetes worldwide continues to rise. Diabetes can cause various complications, seriously endangering human health and affecting quality of life. Studies have found that flavonoids in *Russiania rubra* leaves can reduce blood glucose, liver glycogen, and malondialdehyde (MDA) levels in diabetic model mice induced by alloxan or streptozotocin, while increasing insulin, C-peptide levels, and superoxide dismutase (SOD) activity. Its hypoglycemic mechanism is related to improving the state and antioxidant capacity of damaged pancreatic β-cells. Both the alcoholic extract and phlorizin of *Russiania rubra* leaves have good lipid-lowering activity, and phlorizin can significantly inhibit the expression of the cholesterol synthesis rate-limiting enzyme—hydroxymethylglutaryl-CoA-R (HMG-CoA-R)—and promote cytochrome P450. 450 The expression of the CYP7A1 enzyme, a member of the CYP7A1 family, reduces serum total cholesterol levels. Furthermore, researchers observed the effects of different extracts of *Russula ovata* on the tension of isolated rat thoracic aortic vascular rings. They found no effect on basal vascular rings, but all had varying degrees of vasodilatory effects on vascular rings pre-constricted by potassium chloride, with the n-butanol fraction showing the strongest vasodilatory effect, suggesting a significant antihypertensive effect.

[0004] Chinese patent application 201810856386.4 discloses the use of *Russula ovata* leaves and their extracts in the preparation of antihypertensive drugs. This invention is the first to study the effects of different extracts from *Russula ovata* leaves (total extract, water extract, n-butanol extract, dichloromethane extract, and petroleum ether extract) on the tension of isolated rat thoracic aortic vascular rings, and compares this with *Russula ovata* fruit. The results show that different extracts from both *Russula ovata* fruit and leaves have no significant effect on the basal state of the vascular rings, but all have varying degrees of vasodilatory effects on vascular rings pre-constricted by potassium chloride. Among the extracts from both leaves and fruits, the n-butanol, ethyl acetate, and dichloromethane extracts from *Russula ovata* leaves showed the strongest vasodilatory effects, especially the n-butanol extract. The antihypertensive and vasodilatory effects of *Russula ovata* leaves are significantly stronger than those of *Russula ovata* fruit.

[0005] Chinese Patent 201410259005.6 discloses a method for preparing an extract of *Malus toringo*, comprising the following steps: a) taking *Malus toringo* medicinal material or its aqueous extract, and hydrolyzing it in a mixture of inorganic acid, organic acid, or biological enzyme to obtain a *Malus toringo* hydrolysate; b) concentrating the hydrolysate under reduced pressure and vacuum drying to obtain the *Malus toringo* extract. This invention also provides the uses of the *Malus toringo* extract and pharmaceutical compositions containing *Malus toringo*. This invention studies the hypoglycemic and lipid-lowering mechanisms of the hydrolysate of the Tibetan medicine *Malus toringo* from multiple perspectives. By hydrolyzing the medicinal material or aqueous extract with acid or biological enzymes, the efficacy is significantly enhanced, laying a foundation for the development of *Malus toringo* medicinal materials, a characteristic Tibetan medicinal resource in western Sichuan, and for the development and industrialization of ethnic medicine.

[0006] Phlorizin, an important component of *Lysimachia christinae* extract, possesses various biological activities, including hypoglycemic, hypolipidemic, and antioxidant effects. However, in vivo, phlorizin is hydrolyzed by lactose-phlorizin hydrolase (LPH) into phlorizin, reducing or eliminating its biological activity. Therefore, improving or modifying the structure of flavonoids is an effective way to enhance their bioavailability. Summary of the Invention

[0007] In view of the above-mentioned deficiencies of the prior art, the technical problem to be solved by the present invention is a Russian leaf extract composition, preparation method and application.

[0008] Flavonoids are the main active substances in *Russula ovata*, among which phloridzin is an important active component, accounting for more than 60% of the total flavonoids. Belonging to the dihydrochalcone class of flavonoids, it is a glucoside of phlorizin and possesses antioxidant, antibacterial, hypoglycemic, and anticancer biological activities. However, in vivo, phloridzin is hydrolyzed into phlorizin by lactose-phloridin hydrolase (LPH), reducing or eliminating its biological activity. Therefore, modification is needed to overcome this deficiency. Currently, most existing technologies for modification involve esterification. Since flavonoids, including phloridzin, have poor solubility and stability in both hydrophilic and hydrophobic media, their practical applications are severely limited. While esterification improves solubility in hydrophobic media, the application of *Russula ovata* extract is typically in water-based media, making esterification an unsuitable modification method. Therefore, in this invention, the extract of *Russula ovata* is first extracted and purified, and then modified. The bioactivity of phlorizin, the main component of the *Russula ovata* extract, comes from its phenolic hydroxyl groups. Although it has many hydroxyl groups in its structure, the hydroxyl groups on its pyranose ring form intramolecular hydrogen bonds, resulting in poor water solubility at room temperature. Furthermore, it is easily hydrolyzed into phlorizin under the action of enzymes, thus becoming ineffective. Substituting the hydroxyl group at the 4' position on its B ring can inhibit enzymatic hydrolysis. Therefore, in this invention, the purified extract of *Russula ovata* is modified to improve its water solubility and stability. Then, it is mixed with bitter melon extract, kudzu root extract, hawthorn extract, and astragalus extract, which exert their effects in multiple ways, such as promoting insulin secretion, improving insulin resistance, inhibiting glucosidase activity, antioxidation, and improving blood microcirculation, so that the composition can achieve the best hypoglycemic, hypolipidemic, and hypotensive effects.

[0009] To achieve the above objectives, the present invention provides a composition of Russula ovata extract, comprising the following components in parts by weight: 10-20 parts of Russula ovata extract derivative, 10-15 parts of bitter melon extract, 10-15 parts of kudzu root extract, 5-10 parts of hawthorn extract, and 5-10 parts of astragalus extract.

[0010] The preparation method of the Russian leaf extract derivative includes the following steps:

[0011] X1: Take dried Russian leaves, crush them, add water, heat and extract for 2-4 hours, extract twice, combine the extracts and concentrate to obtain crude extract;

[0012] X2: The crude extract was purified by column chromatography to obtain the Russian leaf extract;

[0013] X3: Add the purified Osmanthus fragrans extract to water, heat to 80-90℃ and stir until completely dissolved, then cool and add propylene oxide dropwise. After the addition is complete, heat to 25-35℃ and stir for 12-18 hours. Add dilute hydrochloric acid to adjust the pH and concentrate the reaction solution to obtain the residue. Dissolve the residue in ethanol and filter. Concentrate the filtrate again and dissolve it in water. Dialyze for 1-2 days and then dry for the next step.

[0014] X4: Add the product from the previous step, potassium carbonate, and phenyltrimethylammonium chloride to toluene, heat to 45~120℃ and stir for 2~5 hours, filter, distill the filtrate to remove the solvent and obtain the residue, add dilute hydrochloric acid to adjust the pH to 5~5.5 and distill again, add alkali to adjust the pH to alkaline and centrifuge and dry to obtain the final product.

[0015] Furthermore, in step X1, the mass ratio of water to Russian sage is 8~10:1.

[0016] Furthermore, the heating temperature range in step X1 is 80~100℃.

[0017] Furthermore, in step X3, the mass ratio of the purified Russian safflower extract to water is 5~8:1.

[0018] Furthermore, the temperature range for cooling in step X3 is 0~5℃.

[0019] Furthermore, in step X3, the pH is adjusted to a neutral level.

[0020] Furthermore, the molecular weight of the dialysis sample is <500.

[0021] A preferred method for preparing the Russian safflower extract derivative includes the following steps:

[0022] X1: Take 10-100 parts by weight of dried Russian leaves, crush them, add water, the mass ratio of water to Russian leaves is 8-10:1, heat to 80-100℃ and extract for 2-4 hours, extract twice, combine the extracts and concentrate to obtain crude extract;

[0023] X2: The crude extract was purified by column chromatography to obtain the Russian leaf extract;

[0024] X3: Add the purified Russian safflower extract to water at a mass ratio of 5-8:1. Heat to 80-90℃ and stir until completely dissolved. Then cool to 0-5℃ and add 0.4-0.5 times the mass of the Russian safflower extract of propylene oxide dropwise. After the addition is complete, heat to 25-35℃ and stir for 12-18 hours. Add dilute hydrochloric acid to adjust the pH to neutral and concentrate the reaction solution to obtain the residue. Dissolve the residue in ethanol and filter. Concentrate the filtrate again and dissolve it in water. Dialyze (Mw < 500) for 1-2 days and then dry for the next step.

[0025] X4: Add 40-100 parts by weight of the product from the previous step, 45-105 parts by weight of potassium carbonate, and 10-20 parts by weight of phenyltrimethylammonium chloride to 300-400 parts by weight of toluene. Heat to 45-120°C and stir for 2-5 hours. Filter, and distill the filtrate to remove the solvent to obtain the residue. Adjust the pH of the residue to 5-5.5 with dilute hydrochloric acid and distill again. Adjust the pH of the obtained residue to alkaline with alkali, centrifuge, and dry to obtain the final product.

[0026] A method for preparing a composition of Russian oleifera leaf extract includes the following steps:

[0027] The extracts of Russian safflower, bitter melon, kudzu root, hawthorn, and astragalus are pulverized, sieved, and then mixed evenly.

[0028] Furthermore, the mesh size of the sieve is 200-300 mesh.

[0029] The present invention also provides the application of the Russian foliage extract composition in the fields of lowering blood sugar, lowering blood lipids, and lowering blood pressure.

[0030] The beneficial effects of this invention are:

[0031] 1. Compared with the prior art, the present invention improves the water solubility and stability of the purified extract of Russian ochre leaves by modifying it.

[0032] 2. Compared with existing technologies, the Russian safflower extract composition prepared by this invention has the effects of lowering blood sugar, lowering blood lipids, and lowering blood pressure, and its efficacy is stable, mild, and highly safe. Detailed Implementation

[0033] Bitter melon extract (extraction ratio 10:1), kudzu root extract (extraction ratio 30:1), hawthorn extract (extraction ratio 10:1), and astragalus extract (extraction ratio 10:1) are all from Xi'an Zhongkeda Biotechnology.

[0034] Dilute hydrochloric acid, 1 mol / L.

[0035] Example 1

[0036] A method for preparing a composition of Russian oleifera leaf extract includes the following steps:

[0037] The following ingredients are prepared by pulverizing 150g of Russian safflower extract derivative, 100g of bitter melon extract, 100g of kudzu root extract, 80g of hawthorn extract, and 60g of astragalus extract, passing them through a 300-mesh sieve, and then mixing them evenly.

[0038] The preparation method of the Russian leaf extract derivative includes the following steps:

[0039] X1: Take 1000g of dried Russian leaves, crush them, add water, the mass ratio of water to Russian leaves is 10:1, heat to 95℃ and extract for 3h, extract twice, combine the extracts and concentrate to obtain crude extract;

[0040] X2: The crude extract was purified by column chromatography to obtain the Russian leaf extract;

[0041] X3: Add the purified Russian safflower extract to water at a mass ratio of 6:1. Heat to 85°C and stir until completely dissolved. Then cool to 0°C and add 0.42 times the mass of the Russian safflower extract of propylene oxide dropwise. After the addition is complete, heat to 30°C and stir for 16 hours. Add dilute hydrochloric acid to adjust the pH to neutral and concentrate the reaction solution to obtain the residue. Dissolve the residue in ethanol and filter. Concentrate the filtrate again and dissolve it in water. Dialyze (Mw < 500) for 1 day and then dry for the next step.

[0042] X4: Add 500g of the product from the previous step, 552g of potassium carbonate, and 188g of phenyltrimethylammonium chloride to 4L of toluene, heat to 100℃ and stir for 4 hours, filter, distill the filtrate to remove the solvent and obtain the residue, add dilute hydrochloric acid to adjust the pH to 5 and then distill again, add alkali to adjust the pH to alkaline and centrifuge, dry and obtain the product.

[0043] Example 2

[0044] Same as in Example 1, except that the amount of the Russian leaf extract derivative added is 100g.

[0045] Example 3

[0046] Same as in Example 1, except that the amount of the Russian leaf extract derivative added is 120g.

[0047] Example 4

[0048] Same as in Example 1, except that the amount of the Russian leaf extract derivative added is 170g.

[0049] Compare with Example 1

[0050] A method for preparing a composition of Russian oleifera leaf extract includes the following steps:

[0051] The following ingredients are prepared by pulverizing 150g of Russian safflower extract, 100g of bitter melon extract, 100g of kudzu root extract, 80g of hawthorn extract, and 60g of astragalus extract, passing them through a 300-mesh sieve, and then mixing them evenly.

[0052] The preparation method of the Russian safflower extract includes the following steps:

[0053] X1 Take 1000g of dried Russian leaves, crush them, add water, the mass ratio of water to Russian leaves is 10:1, heat to 95℃ and extract for 3h, extract twice, combine the extracts and concentrate to obtain crude extract;

[0054] X2 obtained the Russian leaf extract by column chromatography of the crude extract.

[0055] Compare with Example 2

[0056] A method for preparing a composition of Russian oleifera leaf extract includes the following steps:

[0057] The following ingredients are prepared by pulverizing 150g of Russian safflower extract, 100g of bitter melon extract, 100g of kudzu root extract, 80g of hawthorn extract, and 60g of astragalus extract, passing them through a 300-mesh sieve, and then mixing them evenly.

[0058] The preparation method of the Russian safflower extract includes the following steps:

[0059] Take 1000g of dried Russian safflower leaves, pulverize them, add water (water to Russian safflower leaves mass ratio of 10:1), heat to 95℃ and extract for 3h, extract twice, combine the extracts and concentrate to obtain Russian safflower leaf extract.

[0060] Compare with Example 3

[0061] A method for preparing a composition of Russian oleifera leaf extract includes the following steps:

[0062] The following ingredients are prepared by pulverizing 150g of Russian safflower extract derivative, 100g of bitter melon extract, 100g of kudzu root extract, 80g of hawthorn extract, and 60g of astragalus extract, passing them through a 300-mesh sieve, and then mixing them evenly.

[0063] The preparation method of the Russian leaf extract derivative includes the following steps:

[0064] X1: Take 1000g of dried Russian leaves, crush them, add water, the mass ratio of water to Russian leaves is 10:1, heat to 95℃ and extract for 3h, extract twice, combine the extracts and concentrate to obtain crude extract;

[0065] X2: The crude extract was purified by column chromatography to obtain the Russian leaf extract;

[0066] X3: Add the purified Russian safflower extract to water at a mass ratio of 6:1. Heat to 85°C and stir until completely dissolved. Then cool to 0°C and add 0.42 times the mass of the Russian safflower extract of propylene oxide dropwise. After the addition is complete, heat to 30°C and stir for 16 hours. Add dilute hydrochloric acid to adjust the pH to neutral and concentrate the reaction solution to obtain the residue. Dissolve the residue in ethanol and filter. Concentrate the filtrate again and dissolve it in water. Dialyze (Mw < 500) for 1 day and then dry to obtain the final product.

[0067] Test Example 1

[0068] Male Kunming mice, SPF grade, weighing (20±2) g, were used. After 7 days of acclimatization feeding, the mice were randomly divided into groups. They were fasted but allowed water for 12 hours. Each mouse was intraperitoneally injected with alloxan at a dose of 220 mg / kg. The control group and mice were injected with the same volume of physiological saline. They were then fed normally. After 72 hours, blood was collected from the tail vein, and fasting blood glucose was measured using blood glucose test strips. A blood glucose value ≥11.1 mmol / L was considered a successful establishment of the diabetic model. The successfully modeled diabetic mice were divided into three groups: an experimental group (500 mg / kg), a positive control group (metformin hydrochloride 260 mg / kg), and a model group. A blank group was also established. Each group consisted of 10 mice. The model group and the blank group were given the same volume of physiological saline by gavage for 21 consecutive days. On day 0, day 7, and day 21 after gavage administration, mice in each group were fasted for 12 hours but allowed free access to water. Blood was collected from the tail tip, and fasting blood glucose was measured using a glucometer. The results were expressed as mmol / L, and the changes in blood glucose among the mice in each group were compared.

[0069] Table 1. Effects of the Russian safflower extract composition on blood lipid levels in diabetic model mice.

[0070]

[0071] Test Example 2

[0072] Blood was collected from the eyes of mice that had been treated for 21 days in Test Example 1. Serum was separated and tested for serum triglyceride and total cholesterol levels, as detailed in Table 2.

[0073] Table 2. Effects of Russian safflower extract composition on blood lipid levels in diabetic model mice.

[0074]

[0075] Test Example 3

[0076] SHR-grade male spontaneously hypertensive rats, weighing 180-220g, were divided into 6 groups: control group, model group, and experimental group, with 10 rats in each group. Wistar male rats weighing 180-220g served as the normal control group. The hypertensive model SHR rats in the experimental group were administered captopril at a dose of 500mg / kg·d daily via gavage, diluted with 7% physiological saline. The control group received captopril at 5mg / kg·d. The normal and model groups were given the same amount of distilled water. The administration was continued for 30 days, with normal feeding during the administration period. The rats were administered the drug according to the prescribed dosage. On day 14 after gavage, a small animal non-invasive blood pressure monitor was placed on the proximal end of the rat's tail to block or release arterial blood flow. Systolic and diastolic blood pressure were accurately read based on the tail artery pulsation curve and the pressure transducer dose-pressure curve. Specific results are shown in Table 3.

[0077] Table 3. Effects of Russian safflower extract composition on blood lipid levels in diabetic model mice.

[0078]

[0079] The typical symptom of diabetes is persistently high blood sugar levels, accompanied by high blood lipids, leading to various diabetic complications. Lowering high blood sugar levels in diabetic patients is crucial for the treatment and prevention of diabetes and its complications. As shown in Tables 1-3, the extract composition of *Russula ovata* leaves exhibits significant hypoglycemic, hypolipidemic, and hypotensive effects. This is because the flavonoids in *Russula ovata* leaves can reduce blood glucose, liver glycogen, and malondialdehyde (MDA) levels in diabetic model mice induced by alloxan or streptozotocin, while increasing insulin, C-peptide levels, and superoxide dismutase (SOD) activity. Its hypoglycemic mechanism is related to improving the state and antioxidant capacity of damaged pancreatic β-cells. Furthermore, phlorizin significantly inhibits the expression of the cholesterol synthesis rate-limiting enzyme—hydroxymethylglutaryl-CoA (HMG-CoA-R)—and promotes cytochrome P450. 450 The expression of the CYP7A1 enzyme, a member of the CYP7A1 family, reduces serum total cholesterol levels. The antihypertensive mechanism of *Russula ovata* may involve inhibiting the opening of voltage-dependent calcium channels, reducing extracellular calcium influx, and thus lowering intracellular calcium levels. 2+The concentration decreases, thus dilating blood vessels. Bitter melon has a significant hypoglycemic effect, with its main components including saponins, polysaccharides, and polypeptides. Its mechanisms of action are diverse; bitter melon saponins have insulin-like effects, and bitter melon polypeptides can affect insulin secretion. Kudzu root is widely used in cardiovascular and cerebrovascular diseases, with pharmacological effects including lowering blood pressure, blood lipids, and blood sugar. Its main active ingredient is kudzu flavonoids, whose mechanism may be related to inhibiting glucosidase activity, resisting lipid peroxidation, improving vascular endothelial function, and improving blood microcirculation. Total kudzu flavonoids have strong α-glucosidase inhibitory activity, which can enhance the hypoglycemic effect of the combination. Hawthorn and astragalus extracts also have varying degrees of hypoglycemic, hypolipidemic, or hypotensive effects.

[0080] As can be seen from the effects on blood glucose, blood lipids, and blood pressure levels in Tables 1-3, both the control examples and the examples showed good efficacy. However, the effects of control examples 1-3 were not as good as those of example 1. This is because phlorizin, the main flavonoid component in the extract of *Russula ovata*, has poor water solubility and is easily decomposed. Control examples 1-2 only crudely extracted *Russula ovata*, so the content of phlorizin obtained from the final enrichment differed from that in example 1. In control example 2, further purification was carried out, but no modification was performed. Therefore, it not only had poor water solubility but was also easily decomposed by enzymes. In control example 3, water solubility was improved through modification, so the effect was more significant than that of control examples 1-2. However, phlorizin also has the risk of being decomposed. In Example 1, the extract of *Russula ovata* was first extracted and purified, and then modified. The main component of the *Russula ovata* extract, phlorizin, has bioactivity derived from its phenolic hydroxyl groups. Although it has many hydroxyl groups in its structure, the hydroxyl groups on its pyranose ring form intramolecular hydrogen bonds, resulting in poor water solubility at room temperature. Furthermore, it is easily hydrolyzed into phlorizin under the action of enzymes, thus becoming ineffective. Substituting the hydroxyl group at the 4' position on its B ring can inhibit enzymatic hydrolysis. Therefore, in this invention, the purified extract of *Russula ovata* is modified to improve its water solubility and stability. Then, it is mixed with bitter melon extract, kudzu root extract, hawthorn extract, and astragalus extract, which exert their effects in multiple ways, such as promoting insulin secretion, improving insulin resistance, inhibiting glucosidase activity, antioxidation, and improving blood microcirculation, so that the composition can achieve the best hypoglycemic, hypolipidemic, and hypotensive effects.

[0081] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A composition of Russian ochre leaf extract, characterized in that, It is prepared from the following components in parts by weight: 10-20 parts of *Russianthus chinensis* leaf extract derivative, 10-15 parts of bitter melon extract, 10-15 parts of kudzu root extract, 5-10 parts of hawthorn extract, and 5-10 parts of astragalus extract; the preparation method of the *Russianthus chinensis* leaf extract derivative includes the following steps: X1: Take 10-100 parts by weight of dried *Russianthus chinensis* leaves, pulverize them, add water, the mass ratio of water to *Russianthus chinensis* leaves is 8-10:1, heat to 80-100℃ and extract for 2-4 hours, extract twice, combine the extracts and concentrate to obtain crude extract; X2: The crude extract is subjected to column chromatography to obtain purified *Russianthus chinensis* leaf extract; X3: Add the purified Osmanthus fragrans extract to water at a mass ratio of 5-8:

1. Heat to 80-90°C and stir until completely dissolved. Then cool to 0-5°C and add 0.4-0.5 times the mass of Osmanthus fragrans extract in propylene oxide dropwise. After the addition is complete, heat to 25-35°C and stir for 12-18 hours. Add dilute hydrochloric acid to adjust the pH to neutral and concentrate the reaction solution to obtain the residue. Dissolve the residue in ethanol and filter. Concentrate the filtrate again and dissolve it in water. Dialyze under conditions of Mw < 500 for 1-2 days and then dry for the next step. X4: Add 40-100 parts by weight of the product from the previous step, 45-105 parts by weight of potassium carbonate, and 10-20 parts by weight of phenyltrimethylammonium chloride to 300-400 parts by weight of toluene. Heat to 45-120°C and stir for 2-5 hours. Filter, and distill the filtrate to remove the solvent to obtain the residue. Adjust the pH of the residue to 5-5.5 with dilute hydrochloric acid and distill again. Adjust the pH of the obtained residue to alkaline with alkali, centrifuge, and dry to obtain the final product.

2. A method for preparing the Russian safflower extract composition as described in claim 1, characterized in that, The process includes the following steps: pulverizing and sieving the extracts of Rhododendron simsii, bitter melon extract, kudzu root extract, hawthorn extract, and astragalus extract, and then mixing them evenly.

3. The method for preparing the Russian safflower extract composition as described in claim 2, characterized in that, The sieve mesh size is 200-300 mesh.

4. The use of the Russian safflower extract composition as described in claim 1 in the preparation of hypoglycemic, hypolipidemic, and hypotensive drugs.

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