A pesticide composition containing Acynonapyr

Abstract

The present invention belongs to the field of pesticide technology and relates to a pesticide composition containing acenopyr. The active ingredients of the pesticide composition include active ingredient A and active ingredient B. Active ingredient A is acenopyr, and active ingredient B is selected from any one of nicofluprole, trifluoxetine, and fenazaquin. The pesticide composition or its formulation of the present invention can enhance efficacy, expand the acaricide spectrum, reduce usage costs, reduce dosage, extend the duration of efficacy, and delay the development of drug resistance.

Description

[0001] This invention application is a divisional application with application number 2023103330229, application date March 31, 2023, and invention name “A pesticide composition containing Acynonapyr”. Technical Field

[0002] The present invention relates to the technical field of pesticide compounding, in particular to a pesticide composition containing Acynonapyr and application thereof. Background Art

[0003] Mites belong to the phylum Arthropoda, class Arachnida, and subclass Acari. They are diverse in morphology, habits, and habitats, with a wide distribution, strong adaptability, and a wide variety of species. An estimated 300,000 species exist worldwide, second only to the class Insecta.

[0004] Acynonapyr, a novel acaricide with a cyclic amine backbone, developed by Japan Soda Co., Ltd. (CAS registration number 1332838-17-1), acts on inhibitory glutamate receptors (IGluRs), interfering with neurotransmission in mites. This deprives muscle cells of their ability to move, resulting in a paralysis-like state. This in turn affects feeding and movement, ultimately killing the mites. It has excellent control effectiveness against mites in fruit trees, vegetables, and tea plants.

[0005] Pyflubumide is a new carboxanilide acaricide discovered and developed by Nippon Nohaku Co., Ltd. It has significant inhibitory activity against mitochondrial complex II of pest mites. CAS registration number: 926914-55-8.

[0006] Nicofluprole is a new fluorochemical insecticide from the phenylpyrazolonicotinamide family, successfully developed and marketed by Bayer. It has high insecticidal activity against Lepidoptera, Hemiptera, and Acarina pests. CAS registration number: 1771741-86-6.

[0007] Trifluanidazole, trifluanidazole is a new compound independently developed by Shandong Zhongnong United Biotechnology Co., Ltd. CAS registration number is 2074661-82-6.

[0008] Quinazaquin is a quinazoline acaricide developed by Dow AgroSciences. It targets the mitochondria and chromosomes of insect cells, acting as both a contact and stomach poison against pest mites. It is effective against eggs and active mites of various mite species, including Panonychus and Tetranychus. CAS registration number: 120928-09-8.

[0009] Phytophagous mites are one of the most common plant pests. Small in size, they mostly live in dense colonies on the undersides of crop leaves, sucking and causing significant yield losses for fruit trees, cotton, vegetables, and ornamental plants. Phytophagous mites include spider mites (Tetranychidae), gall mites (Eriophyoidea), and root mites (Rhizoglyphus), with spider mites and gall mites being the most damaging. Chemical control is a key component of comprehensive mite management. Reasonable compounding or mixing of chemical agents has the potential to expand the insecticide spectrum, improve control efficacy, extend the appropriate application period, reduce dosage, reduce pesticide damage, minimize residues, and delay the development of pesticide resistance in pests. The applicants were surprised to discover, through indoor toxicity experiments and field efficacy tests, that the combination of Acynonapyr with fenpyroximate, Nicofluprole, trifluoxetine, and fenazaquin exhibits significant synergistic effects, and that the resulting compounded acaricide compositions and their applications have not been reported to date. Summary of the Invention

[0010] Based on the above situation, the purpose of the present invention is to provide a pesticide composition containing Acynonapyr and its preparation, which are mainly used to control herbivorous mites. The acaricidal composition or its preparation can enhance the efficacy, reduce the dosage, and at the same time prolong the lasting effect and delay the development of drug resistance.

[0011] In order to achieve the above object, the present invention adopts the following technical solution: a pesticide composition containing Acynonapyr, comprising an active ingredient A, Acynonapyr, and an active ingredient B selected from any one of fenpyroximate, Nicofluprole, trifluoxetine, and fenazaquin;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 35:1;

[0013] Furthermore, the mass ratio of Acynonapyr to Fenpyrad is 1:20 to 20:1;

[0014] Furthermore, the mass ratio of Acynonapyr to Fenpyrad is 1:35, 1:25, 1:20, 1:15, 1:10, 1:5, 1:1, 11:2, 7:2, 15:1, 10:1, 20:1, 25:1, and 35:1;

[0015] Furthermore, the mass ratio of Acynonapyr to Fenpyrad is 1:20, 1:15, 1:10, 1:5, 1:1, 11:2, 7:2, 15:1, 10:1, and 20:1;

[0016] Furthermore, the mass ratio of Acynonapyr to Fenpyrad is 1:15 to 20:1;

[0017] Furthermore, the mass ratio of Acynonapyr to Fenpyrad is 1:10 to 10:1;

[0018] Furthermore, the mass ratio of Acynonapyr to Nicofluprole is 1:30 to 28:1;

[0019] Furthermore, the mass ratio of Acynonapyr to Nicofluprole is 1:20 to 20:1;

[0020] Furthermore, the mass ratio of Acynonapyr to Nicofluprole is 1:30, 1:25, 1:20, 1:14, 1:8, 1:5, 1:3, 5:1, 9:1, 15:1, 20:1, and 28:1;

[0021] Furthermore, the mass ratio of Acynonapyr to Nicofluprole is 1:20, 1:14, 1:8, 1:5, 1:3, 5:1, 9:1, 15:1, and 20:1;

[0022] Furthermore, the mass ratio of Acynonapyr to Nicofluprole is 1:8 to 20:1;

[0023] Furthermore, the mass ratio of Acynonapyr to Trifluoxetine is 1:30 to 30:1;

[0024] Furthermore, the mass ratio of Acynonapyr to Trifluoxetine is 1:30, 1:20, 1:16, 1:7, 5:3, 6:1, 14:1, 20:1, and 30:1;

[0025] Furthermore, the mass ratio of Acynonapyr to Trifluoxetine is 1:20 to 20:1;

[0026] Furthermore, the mass ratio of Acynonapyr to Trifluoxetine is 1:20 to 14:1;

[0027] Furthermore, the mass ratio of Acynonapyr to Trifluoxetine is 1:20, 1:16, 1:7, 5:3, 6:1, and 14:1;

[0028] Furthermore, the mass ratio of Acynonapyr to Quinazac is 1:25 to 24:1;

[0029] Furthermore, the mass ratio of Acynonapyr to Fenazaquin is 1:25, 1:15, 1:8, 1:1, 13:2, 18:1, and 24:1;

[0030] Furthermore, the mass ratio of Acynonapyr to Fenazaquin is 1:20 to 20:1;

[0031] Furthermore, the mass ratio of Acynonapyr to Quinazac is 1:15 to 18:1;

[0032] Furthermore, the mass ratio of Acynonapyr to Fenazaquin is 1:15, 1:8, 1:1, 13:2, and 18:1;

[0033] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1 to 95 wt%;

[0034] Based on the total weight of the acaricidal composition being 100 wt%, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1 to 95 wt%;

[0035] Furthermore, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 5 to 80 wt%;

[0036] In a preferred embodiment, the sum of the contents of Acynonapyr and Fenpyraclostrobin in the pesticide composition is 5 to 70 wt%, for example, the sum of the contents of Acynonapyr and Fenpyraclostrobin is 6%, 18%, 24% or 40%;

[0037] In a preferred embodiment, the sum of the content of Acynonapyr and Nicofluprole in the pesticide composition is 5 to 70 wt%, for example, the sum of the content of Acynonapyr and Nicofluprole in the pesticide composition is 5.5%, 15%, 32% or 40%;

[0038] In a preferred embodiment, the sum of the contents of Acynonapyr and trifluoxetine in the pesticide composition is 5 to 70 wt %, for example, the sum of the contents of Acynonapyr and trifluoxetine in the pesticide composition is 15%, 20%, 24% or 44%;

[0039] In a preferred embodiment, the sum of the contents of Acynonapyr and fenazaquin in the pesticide composition is 5 to 70 wt%, for example, the sum of the contents of Acynonapyr and fenazaquin in the pesticide composition is 10%, 20%, 25%, 30% or 4.5%;

[0040] A pesticide composition containing Acynonapyr, wherein the pesticide composition further comprises, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist, or a carrier;

[0041] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0042] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0043] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0044] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0045] Disintegrants: The disintegrants are selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0046] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0047] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0048] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0049] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0050] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0051] Synergists are selected from synergist, piperonyl butoxide; and / or

[0052] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0053] Furthermore, the pesticide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is selected from a solid formulation and / or a liquid formulation;

[0054] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0055] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0056] Furthermore, the liquid preparation is selected from emulsifiable concentrates, suspension concentrates and / or aqueous emulsions, and the solid preparation is selected from wettable powders;

[0057] Use of a pesticide composition containing Acynonapyr for controlling phytophagous mites;

[0058] Furthermore, the phytophagous pest mites are Tetranychus, Acaridae and / or Eupodidae pest mites;

[0059] Furthermore, the herbivorous mites are Tetranychus pests;

[0060] Furthermore, the pest mite of the Tetranychus family is Tetranychus cinnabarinus, Tetranychus urticae, Panonychus citri, Tetranychus kanzawa, Tetranychus truncatus and / or Panonychus malus;

[0061] Furthermore, the harmful mites of the Tetranychus family are Tetranychus cinnabarinus and Tetranychus truncatus;

[0062] Use of a pesticide composition containing Acynonapyr for controlling phytophagous mites;

[0063] Furthermore, the phytophagous pest mite is spider mite on cotton;

[0064] Furthermore, the spider mites on the cotton are Tetranyehus cinnatarinus (Boisduval), Tetranyehus truncatus Ehara, Tetranychus two-spotted spider mite (Tetmnychusurticae koch), Tetranychus turkestani (Ugarov et Nikolski), Tetranychus dunhuangensis (wang), etc.

[0065] Furthermore, the acaricidal composition and / or its preparation is applied in an effective dose to the harmful mites to be controlled or the medium where they grow.

[0066] Beneficial effects of the present invention:

[0067] (1) Acynonapyr can be combined with any of fenvalerate, Nicofluprole, trifluoxetine, and fenazaquin to achieve a significant synergistic effect within a certain range and effectively control various mites.

[0068] (2) It reduces the amount of pesticides used, can delay the development of pesticide resistance in pest mites, and is safe, efficient, and environmentally friendly. DETAILED DESCRIPTION

[0069] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0070] Indoor toxicity

[0071] Examples Reference Pesticide Indoor Bioassay Test Guidelines Part 13: Leaf Disk Spray Method NY / T 1154.13-2008; Part 7: Determination of Combined Effects of Mixtures NY / T 1154.7-2006

[0072] Test material preparation

[0073] Acynonapyr TC, Fenpyrad Amine TC, Nicofluprole TC, Trifluoxetine TC, and Fenazaquin TC are all provided by the Group's R&D Center.

[0074] Test targets: Tetranychus cinnabarinnum Boisduval, Tetranychus truncatus Ehara;

[0075] Select female adult mites or nymphs raised indoors and in a consistent physiological state. The temperature is (25 ± 1)°C, the relative humidity is 65% ± 5%, and the photoperiod is 16 / 8h (L / D).

[0076] Select cotton leaves with uniform growth, use a hole puncher to make leaf discs with a diameter of 2 cm, place agar in a culture dish to keep it moist, place filter paper on it, and place leaf discs on the filter paper, with 3 leaf discs per dish, and inoculate adult mites or nymphs raised indoors onto the leaf discs, with 10 to 15 mites on each leaf disc.

[0077] Preparation: Dissolve the original drug in a suitable organic solvent and dilute with a 0.1% Tween-80 aqueous solution. Prepare separate single-dose stock solutions and design five groups of mixes based on the mixing purpose and drug activity. Prepare five series of mass concentrations for each single dose and each mix using the same ratio.

[0078] Chemical treatment: Place the petri dish on the bottom plate of a Potter spray tower and spray with a volume of 1 ml. Allow the solution to settle for 1 minute before removing it from the dish and transferring it to rearing conditions. Each treatment should be replicated at least four times, with at least 120 insects tested at each dose. A blank control containing no chemical (including all organic solvents and emulsifiers) should be used.

[0079] Data Statistics and Analysis: 48 hours after treatment, examine the insects for mortality and record the total insect count and the number of dead insects. Calculate the adjusted mortality rate for each treatment based on the survey data.

[0080] Calculate using the following formula, with the results rounded to two decimal places:

[0081]

[0082] Where:

[0083] P——mortality rate, in percentage (%);

[0084] K——indicates the number of dead insects, the unit is head;

[0085] N——represents the total number of insects processed, in heads.

[0086]

[0087] Where:

[0088] P1——adjusted mortality rate, in percentage (%);

[0089] P t ——Treatment mortality rate, expressed in percentage (%);

[0090] P0 - blank control mortality rate, in percentage (%).

[0091] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0092] The data were processed using the probability value analysis method. The LC of the toxicity regression line can be obtained by using the IBM SPSS Statistics 20 statistical analysis system. 50 The activity of the test agent on the biological test material was evaluated by using the b value and its 95% confidence limit.

[0093] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0094]

[0095] Where:

[0096] ATI - measured toxicity index of mixture;

[0097] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0098] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0099] TTI=TI A *P A +TI B *P B

[0100] Where:

[0101] TTI – Theoretical Toxicity Index of Mixtures;

[0102] TI A ——Agent toxicity index;

[0103] P A ——The percentage of agent A in the mixture, in percentage (%);

[0104] TIB ——Toxicity index of agent B;

[0105] P B ——The percentage of agent B in the mixture, in percentage (%).

[0106]

[0107] Where:

[0108] CTC – Co-toxicity coefficient;

[0109] ATI - measured toxicity index of mixture;

[0110] TTI - Theoretical Toxicity Index of Mixture.

[0111] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0112] Example 1

[0113] Determination of the combined toxicity of Acynonapyr with Fenpyraclostrobin, Nicofluprole, and Trifluprole in different ratios against Tetranychus cinnabarinus (female adult mites):

[0114] Table 1 shows that single agents of acynonapyr, fenpyraclostrobin, and nicofluprole exhibited high toxicity against T. cinnabarinus, with nicofluprole showing the greatest control efficacy. Acynonapyr and fenpyraclostrobin exhibited no antagonistic effect against T. cinnabarinus at a ratio of 1:35 to 35:1. In the 1:15 to 25:1 range, the cotoxicity coefficient exceeded 130, indicating a significant synergistic effect. Acynonapyr and nicofluprole exhibited no antagonistic effect against T. cinnabarinus at a ratio of 1:30 to 28:1. In the 1:14 to 20:1 range, the cotoxicity coefficient exceeded 130, indicating a significant synergistic effect. Acynonapyr and trifluoxetine exhibited no antagonistic effect against T. cinnabarinus at a ratio of 1:30 to 30:1. Synergistic effects were observed in the 1:20 to 20:1 range.

[0115] Table 1 Combined toxicity test of different ratios of Acynonapyr with fenvalerate, Nicofluprole and Trifluprole against Tetranychus cinnabarinus (female adult mites)

[0116]

[0117]

[0118] Example 2

[0119] Determination of the combined toxicity of different ratios of aceynonapyr and quinazaquin against Tetranychus truncatus (nymphs):

[0120] Table 2 shows that each single agent of acynonapyr and fenazaquin exhibited high toxicity against nymphs of Tetranychus truncatus. Acynonapyr and fenazaquin exhibited synergistic effects against Tetranychus truncatus at a ratio of 1:25 to 18:1. When the mass ratio of acynonapyr to fenazaquin was 1:1, the co-toxicity coefficient was 229.964, indicating significant synergy.

[0121] Table 2 Combined toxicity test of different ratios of Acynonapyr and Quinazacol to Tetranychus truncatus (nymphs)

[0122]

[0123] Example 3

[0124] Determination of the combined toxicity of Acynonapyr with Fenpyraclostrobin and Nicofluprole in different ratios against Tetranychus truncatus (female adult mites):

[0125] Table 3 shows that each single agent of acynonapyr, fenpyraclostrobin, and nicofluprole exhibited high toxicity against Tetranychus truncatus. Acynonapyr and fenpyraclostrobin exhibited synergistic effects against Tetranychus truncatus at a mass ratio of 1:20 to 20:1. At a mass ratio of 2:3, the co-toxicity coefficient was 194.006, indicating significant synergy. Acynonapyr and nicofluprole exhibited synergistic effects against Tetranychus truncatus at a mass ratio of 1:20 to 20:1. At a mass ratio of 5:1, the co-toxicity coefficient was 207.189, indicating significant synergy.

[0126] Table 3 Combined toxicity test of different ratios of Acynonapyr, Fenpyraclostrobin and Nicofluprole against Tetranychus truncatus (female adult mites)

[0127]

[0128] Preparation Example

[0129] Preparation Example 1: 24% Acynonapyr·Fenpyraclostrobin suspension (18:6)

[0130] Preparation formula: 18% Acynonapyr, 6% Cypermethrin, 3% Castor Oil Polyoxyethylene Ether Phosphate, 1.5% Polycarboxylate, 3% Sodium Lauryl Sulfate, 0.8% Sodium Paraben, 0.3% Xanthan Gum, 5% Ethylene Glycol, 1% Magnesium Aluminum Silicate, 0.2% Silicone, and water to make up the balance;

[0131] Preparation method: According to the formula ratio of the embodiment, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0132] Preparation Example 2: 32% Acynonapyr·Nicofluprole Suspension (27:5)

[0133] Preparation formula: 27% Acynonapyr, 5% Nicofluprole, 1.5% polycarboxylate, 3% sodium polyoxyethylene fatty alcohol ether sulfate, 1% sodium dioctyl sulfosuccinate, 0.5% sodium p-hydroxybenzoate, 0.1% sorbic acid, 0.2% xanthan gum, 4% ethylene glycol, 1% glycerin, 0.5% magnesium aluminum silicate, 0.1% silicone defoaming agent, and water to make up the balance;

[0134] Preparation method: Same as Preparation Example 1.

[0135] Preparation Example 3: 24% Acynonapyr·Trifluoxetine Suspension (15:9)

[0136] Preparation formula: 15% Acynonapyr, 9% Trifluoxetine, 3% Castor Oil Polyoxyethylene Ether Phosphate, 3% Sodium Lauryl Sulfate, 0.8% Sodium Paraben, 0.3% Xanthan Gum, 5% Ethylene Glycol, 1% Magnesium Aluminum Silicate, 0.2% Silicone, and water to make up the balance;

[0137] Preparation method: Same as Preparation Example 1.

[0138] Preparation Example 4: 30% Acynonapyr·Quinazac suspension (15:15)

[0139] Preparation formula: 15% Acynonapyr, 15% Quinazaquin, 3% Castor Oil Polyoxyethylene Ether Phosphate, 1% Polycarboxylate, 2% Sodium Lauryl Sulfate, 0.5% Kasson, 0.2% Xanthan Gum, 5% Ethylene Glycol, 1% Magnesium Aluminum Silicate, 0.2% Silicone Defoamer, and water to make up the balance;

[0140] Preparation method: Same as Preparation Example 1.

[0141] Preparation Example 5: 40% Acynonapyr·Azolam wettable powder (20:20)

[0142] Preparation formula: 20% Acynonapyr, 20% Cypermethrin, 2% Sodium Lauryl Sulfate, 9% Sodium Dodecylbenzenesulfonate, 8% Polycarboxylate, 7% Starch, 4% White Carbon Black, Kaolin makes up the balance;

[0143] Preparation method: According to the formula ratio of the embodiment, the active ingredients are added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and then air flow crushed and mixed again to obtain the wettable powder product.

[0144] Preparation Example 6: 44% Acynonapyr·Trifluoxetine Wettable Powder (24:20)

[0145] Preparation formula: 24% Acynonapyr, 20% Trifluoxetine, 10% Sodium Dodecylbenzenesulfonate, 8% Polycarboxylate, 2% Sodium Lauryl Sulfate, 5% Sucrose, 2% White Carbon Black, and Bentonite to make up the balance;

[0146] Preparation method: Same as Preparation Example 5.

[0147] Preparation Example 7: 6% Acynonapyr·Azolam EC (4:2)

[0148] Preparation formula: 4% Acynonapyr, 2% Cypermethrin, 20% Propylene Carbonate, 5% Calcium Dodecylbenzenesulfonate, 4% Corn Oil, 15% Fatty Alcohol Polyoxyethylene Ether, and Methyl Oleate to make up the balance;

[0149] Preparation method: Add the active ingredients into a carrier according to the formula ratio of the embodiment, add a surfactant and other functional additives thereto, stir and mix them evenly in a stirring mixing kettle to obtain an emulsifiable concentrate product.

[0150] Preparation Example 8: 5.5% Acynonapyr·Nicofluprole emulsifiable concentrate (4:1.5)

[0151] Preparation formula: 4% Acynonapyr, 1.5% Nicofluprole, 20% propylene carbonate, 5% calcium dodecylbenzenesulfonate, 15% fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0152] Preparation method: Same as Preparation Example 7.

[0153] Preparation Example 9: 4.5% Acynonapyr·Quinazacuin EC (2:2.5)

[0154] Preparation formula: 2% Acynonapyr, 2.5% Quinazaquin, 17% Propylene Carbonate, 5% Sodium Dodecylbenzenesulfonate, 5% Soybean Oil, 14% Fatty Alcohol Polyoxyethylene Ether, and Methyl Oleate to make up the balance;

[0155] Preparation method: Same as Preparation Example 7.

[0156] Preparation Example 10: 18% Acynonapyr·Fenpyraclostrobin aqueous emulsion (10:8)

[0157] Preparation formula: 10% Acynonapyr, 8% Cyclohexanil, 10% xylene, 10% cyclohexanone, 2% calcium dodecylbenzenesulfonate, 8% ethylene oxide-propylene oxide copolymer, 4% ethylene glycol, 1.3% glycerol, 0.05% silicone defoamer, 0.1% xanthan gum, 0.01% kason, and water to make up the balance;

[0158] Preparation method: According to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase. Deionized water and antifreeze are mixed together to form a uniform water phase; the water phase is added to the oil phase, stirred evenly, and then a defoaming agent is added and high-speed shearing is performed to form a well-dispersed water emulsion product.

[0159] Preparation Example 11: 15% Acynonapyr·Nicofluprole aqueous emulsion (12:3)

[0160] Preparation formula: 12% Acynonapyr, 3% Nicofluprole, 5% xylene, 15% cyclohexanone, 7% styrylphenol polyoxyethylene ether, 1% alkylphenol ether phosphate, 5% ethylene glycol, 0.05% silicone defoamer, 0.1% xanthan gum, 0.01% methyl paraben, and water to make up the balance;

[0161] Preparation method: Same as Preparation Example 10.

[0162] Example 4 Field Trial Control of Cotton Spider Mites

[0163] Test crops: cotton (Zhongmian EB001);

[0164] Test subjects: Tetranychus cinnabarinus, Tetranychus truncatus;

[0165] Test location: cotton planting base in Yutai County, Jining City, Shandong Province. The previous crop was garlic. The soil fertility of the test site was medium, the terrain was flat, the fertility was uniform, and the irrigation conditions were good.

[0166] Experimental treatment: Each plot was randomly arranged in blocks, with a plot area of 20m 2 , repeated 4 times, the spraying equipment was WS-16D Guardian electric sprayer, the nozzle was a single fan-shaped mist nozzle, the working pressure was 0.15-0.4Mpa, the front and back of the cotton leaves were sprayed evenly, the water consumption per mu was 50kg for foliar spray, and the pesticide was applied only once during the entire test process.

[0167] The weather was good during the test. On the day of the test, the average daily temperature was 24°C, the highest temperature was 29°C, the lowest temperature was 17°C, and the relative humidity was 70%.

[0168] Survey method: Investigate the insect population base before applying the pesticide. Hang 40 leaves on fixed plants in each plot for investigation. The number of mites before treatment should be no less than 500. Investigate the number of live mites 3 days and 14 days after applying the pesticide.

[0169] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0170]

[0171]

[0172] During the experiment, cotton in each treatment plot grew well and no pesticide damage was observed in any treatment.

[0173] Test results:

[0174] Test results of each treatment agent for controlling cotton spider mites:

[0175] Table 4 Field test results of different test agents on cotton spider mites

[0176]

[0177] Note: The control efficacy (%) in the above table is the average of each replicate. Lowercase letters represent significant differences at the 5% level, and uppercase letters represent significant differences at the 1% level.

[0178] Table 5 Field test results of different test agents on cotton spider mites

[0179]

[0180] Note: The control efficacy (%) in the above table is the average of each replicate. Lowercase letters represent significant differences at the 5% level, and uppercase letters represent significant differences at the 1% level.

[0181] Safety was assessed based on the presence of pesticide damage on cotton leaves, buds, flowers, and bolls in each plot. Field surveys revealed no adverse reactions to the pesticide on leaves or shoots across all treatments, demonstrating that each treatment was safe for cotton growth at the concentrations used in the study.

[0182] The field test results for different test agents against cotton spider mites in Tables 4 and 5 demonstrate that the acaricide compositions obtained by combining Acynonapyr with fenpyroximate, Nicofluprole, trifluoxetine, and fenazaquin exhibit excellent rapid efficacy against cotton spider mites. Three days after application, the control efficacy of each combination exceeded 80%. Fourteen days after application, the efficacy of each combination increased, reaching over 90% in all groups, demonstrating good long-term effectiveness.

[0183] Through indoor toxicity determination and tests on cotton in the field, the acaricidal composition of the present invention, which is a combination of Acynonapyr and any one of fenpyroximate, Nicofluprole, trifluoxetine, and fenazaquin, shows good control effect on phytophagous pest mites.

[0184] The acaricidal composition or formulation obtained by the present invention exhibits significant control efficacy, outperforming single agents in delaying the development of resistance and prolonging drug retention. Furthermore, no crop damage was observed with the combined formulation in testing, demonstrating that the acaricidal composition or formulation, while enhancing synergistic acaricidal efficacy, can reduce production and usage costs and is safe for crops.

[0185] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition containing Acynonapyr, characterized in that: The active ingredient A is Acynonapyr, and the active ingredient B is Nicofluprole. The mass ratio of Acynonapyr to Nicofluprole is 1:20 to 20:

1.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of Acynonapyr to Nicofluprole is 1:8-20:

1.

3. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition as 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1 to 95 wt%.

4. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition as 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 5 to 80 wt%.

5. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

6. The pesticide composition according to claim 5, characterized in that The pesticide composition can be prepared into an agriculturally acceptable formulation, and the formulation is selected from a solid formulation and / or a liquid formulation.

7. The pesticide composition according to claim 6, characterized in that The liquid preparation is selected from emulsifiable concentrates, suspensions, and aqueous emulsions, and the solid preparation is selected from wettable powders.

8. Use of the pesticide composition according to any one of claims 1 to 7 for controlling phytophagous pest mites, characterized in that: The herbivorous pest mites are Tetranychus cinnabarinus and Tetranychus truncatus.

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