An anti-fatigue composition comprising rice bran fatty alkanol and plant components

Abstract

The present invention relates to the technical field of health foods, and more particularly to an anti-fatigue composition comprising rice bran fatty alkanols and plant components. The composition comprises rice bran fatty alkanols and plant components, and the addition of wolfberry polysaccharides imparts excellent anti-fatigue effects to the composition. The anti-fatigue effects are further enhanced by the addition of whey protein, collagen peptides, crystalline fructose, vitamin B1, taurine, and β-nicotinamide mononucleotide.

Description

Technical Field

[0001] The invention relates to the technical field of health-care foods, and in particular to an anti-fatigue composition prepared by compounding rice bran fatty alkanol and plant components. Background Art

[0002] Fatigue is a common physiological phenomenon and a complex physiological and biochemical process. Persistent or severe fatigue not only disrupts normal life but can also lead to endocrine disorders, decreased immunity, and even organic diseases, threatening health. Fatigue is often accompanied by changes in energy substances, metabolites, hormone levels, cellular metabolism regulatory enzymes, and antioxidant system enzymes. Essentially, it is a loss of homeostasis in the internal environment that causes discomfort.

[0003] Fatigue is a state of reduced function and discomfort caused by overuse. It can be divided into two types: central fatigue and peripheral fatigue. Central fatigue is caused by a disturbance in the motor nerve center, resulting in a decrease in motor nerve excitability. Peripheral fatigue manifests as muscle fatigue, or decreased muscle strength.

[0004] Ways to eliminate fatigue include sleep, warm water bath, physical therapy, nutritional supplements and medicine.

[0005] In recent years, many researchers have devoted themselves to anti-fatigue research. Unlike pharmaceuticals, which combat fatigue, food-derived active ingredients have attracted significant attention due to their minimal side effects. However, effective non-pharmaceutical anti-fatigue products are relatively rare, and their effectiveness remains suboptimal. Therefore, there is an urgent need for highly effective anti-fatigue compositions. Summary of the Invention

[0006] The present invention is based on the solution of the above technical problems and provides a technical solution of the present invention. The present invention adopts rice bran fatty alkanol and plant components to compound, and adds wolfberry polysaccharide to give the composition excellent anti-fatigue effect, and adds whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, and β-nicotinamide mononucleotide to further improve the anti-fatigue effect.

[0007] The technical solution of the present invention uses rice bran fatty alkanol and walnut oil to form a rice bran fatty alkanol and walnut oil coating, which enhances the anti-fatigue effect of the composition, making the active ingredients easier to store and more effective. The present invention creatively selects to use the rice bran fatty alkanol and walnut oil coating to compound with wolfberry polysaccharides, optimizes the dosage ratio of the two, and produces a mutually reinforcing enhancement of the anti-fatigue function of the composition. At the same time, in order to enhance the storage stability of the composition and enhance the effectiveness of the composition during the shelf life, the present invention selects to coat the rice bran fatty alkanol and walnut oil with tragacanth gum and β-cyclodextrin. The combined coating of the two can significantly enhance the anti-fatigue effect of the rice bran fatty alkanol and walnut oil coating.

[0008] On the one hand, the present invention provides an anti-fatigue composition compounded with rice bran fatty alkanol and plant components, the preparation raw materials including: a coating of rice bran fatty alkanol and walnut oil, honeysuckle extract, peony bark extract, whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, β-nicotinamide mononucleotide and wolfberry polysaccharide.

[0009] Furthermore, the anti-fatigue composition of rice bran fatty alkanol and plant components provided by the present invention comprises, in parts by mass, 1.5-5 parts of a coating of rice bran fatty alkanol and walnut oil, 0.8-4.5 parts of honeysuckle extract, 1-5 parts of peony bark extract, 30-40 parts of whey protein, 10-25 parts of collagen peptide, 8-22 parts of crystalline fructose, 0.3-2 parts of vitamin B1, 0.2-1.5 parts of taurine, 4-10 parts of β-nicotinamide mononucleotide, and 1-3 parts of wolfberry polysaccharide.

[0010] Furthermore, the amount of the coating of rice bran fatty alkanol and walnut oil is 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts, 4.8 parts, 5 parts or the range between any two of them.

[0011] Furthermore, the amount of the honeysuckle extract is 0.8 parts, 1 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts, or a range between any two of them.

[0012] Furthermore, the amount of the Paeonia suffruticosa Andrews extract is one of 1 part, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts, 5 parts, or a range between any two of them.

[0013] Furthermore, the amount of whey protein is 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts, 40 parts or a range between any two of them.

[0014] Furthermore, the amount of the collagen peptide is 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts or a range between any two of them.

[0015] Furthermore, the amount of the crystalline fructose is one of 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, or a range between any two of them.

[0016] Furthermore, the amount of vitamin B1 is 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts or a range between any two of them.

[0017] Furthermore, the amount of taurine is 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, or a range between any two of them.

[0018] Furthermore, the amount of β-nicotinamide mononucleotide is 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, or a range between any two of them.

[0019] Furthermore, the amount of the wolfberry polysaccharide is one of 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, or a range between any two of them.

[0020] Furthermore, the rice bran fatty alkanol and walnut oil coating is obtained by coating rice bran fatty alkanol and walnut oil under the action of tragacanth gum and beta-cyclodextrin.

[0021] In order to further enhance the anti-fatigue effect of the composition, the mass ratio of the rice bran fatty alkanol and walnut oil is (2.5-4):1; further, the mass ratio of the two is selected to be (2.6-4):1, (2.6-3.8):1, (2.7-3.6):1, (2.8-3.6):1, (2.9-3.6):1 or (3-3.5):1.

[0022] In order to make the active ingredients easier to store and better exert their effect, the mass ratio of tragacanth gum to β-cyclodextrin is (3-5):1; further selected as (3.1-4.9):1, (3.2-4.8):1, (3.3-4.6):1, (3.5-4.5):1 or (3.7-4.2):1.

[0023] Furthermore, an emulsifier is also used in the preparation process of the rice bran fatty alkanol and walnut oil coating.

[0024] Furthermore, the emulsifier includes Tween-80 and Tween-40.

[0025] In order to enhance the stability of the coating and the performance of the active ingredients, the present invention selects a mass ratio of Tween-80 and Tween-40 of (1-2):1; further selects a mass ratio of the two of (1.1-2):1, (1.2-2):1, (1.2-1.9):1, (1.3-1.8):1 or (1.4-1.7):1.

[0026] Furthermore, the rice bran fatty alkanol and walnut oil coating is obtained by coating rice bran fatty alkanol and walnut oil in the presence of tragacanth gum and β-cyclodextrin in combination with Tween-80 and Tween-40.

[0027] Furthermore, the preparation method of the coating of rice bran fatty alkanol and walnut oil comprises the following steps:

[0028] Step A: Heat and melt rice bran fatty alkanol, add walnut oil, Tween-80 and Tween-40, mix well to obtain an oil phase;

[0029] Step B: heating and mixing tragacanth gum, β-cyclodextrin and water to obtain an aqueous phase;

[0030] Step C: adding the oil phase to the water phase, mixing evenly, and spray drying to obtain a coating of rice bran fatty alkanol and walnut oil.

[0031] Furthermore, the preparation method of the coating of rice bran fatty alkanol and walnut oil comprises the following steps:

[0032] Step A: In an oil phase pot, heat and melt 2.5-4 times the mass of rice bran fatty alkanol relative to walnut oil, add walnut oil and Tween-80 and Tween-40 in a mass ratio of (1-2):1, mix well, and obtain an oil phase; wherein the mass ratio of walnut oil to Tween-40 is 1:(1.5-5);

[0033] Step B: Adding tragacanth gum and β-cyclodextrin in a mass ratio of (3-5):1 and water to an aqueous phase pot, heating and mixing uniformly to obtain an aqueous phase; wherein the mass ratio of walnut oil to β-cyclodextrin is 1:(2.5-5), and the mass ratio of walnut oil to water is 1:(50-200);

[0034] Step C: adding the oil phase to the water phase, mixing evenly, and spray drying to obtain a coating of rice bran fatty alkanol and walnut oil.

[0035] In order to further enhance the anti-fatigue effect of the composition, the mass ratio of the rice bran fatty alcohol and walnut oil coating to wolfberry polysaccharide is (1-2):1, and the mass ratio of the two is further selected as (1.1-2):1, (1.1-1.9):1, (1.2-1.9):1, (1.2-1.8):1, (1.3-1.8):1 or (1.4-1.7):1.

[0036] On the other hand, the present invention also provides a method for preparing an anti-fatigue composition of rice bran fatty alkanol and plant components, comprising: weighing raw materials and mixing the raw materials in a mixer.

[0037] Furthermore, the preparation method also includes a packaging step.

[0038] On the other hand, the present invention also provides an application, including the application of the anti-fatigue composition in the preparation of an anti-fatigue preparation.

[0039] Beneficial effects:

[0040] The present invention adopts rice bran fatty alkanol and plant components in combination, and adds wolfberry polysaccharide to give the composition excellent anti-fatigue effect, and adds whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, and β-nicotinamide mononucleotide to further improve the anti-fatigue effect.

[0041] The present invention uses rice bran fatty alkanol and walnut oil to form a coating of the rice bran fatty alkanol and walnut oil, thereby enhancing the anti-fatigue effect of the composition, making it easier to store the active ingredients and achieving a better effect. The present invention creatively selects to use the coating of the rice bran fatty alkanol and walnut oil in combination with wolfberry polysaccharides, optimizing the dosage ratio of the two, thereby mutually enhancing the anti-fatigue function of the composition. Furthermore, to enhance the storage stability of the composition and the effectiveness of the composition during its shelf life, the present invention selects to coat the rice bran fatty alkanol and walnut oil with tragacanth gum and β-cyclodextrin. The combined coating of the two significantly enhances the anti-fatigue effect of the coating of the rice bran fatty alkanol and walnut oil.

[0042] After the anti-fatigue composition obtained by the present invention was stored for 10 months, a weight-bearing swimming experiment in mice showed that the exhaustive swimming time t value of the mice was as high as 327.38 seconds.

[0043] The anti-fatigue composition containing both the rice bran fatty alkanol and walnut oil coating and wolfberry polysaccharide in the system of the present invention shows a longer exhaustive swimming time t value for mice, and when the mass ratio of the two is (1-2):1, the composition has a better anti-fatigue effect.

[0044] The present invention uses tragacanth gum and β-cyclodextrin to co-coat a coating of rice bran fatty alkanol and walnut oil. The coating exhibits a more stable storage effect and efficacy in the composition (after storage for 10 months). The co-coating of the two can significantly enhance the anti-fatigue effect of the coating of rice bran fatty alkanol and walnut oil.

[0045] The anti-fatigue composition containing the coating of rice bran fatty alkanol and walnut oil and wolfberry polysaccharide in the composition of the present invention shows more favorable blood lactate value, serum urea nitrogen value and muscle glycogen water value of serum samples; tragacanth gum and β-cyclodextrin jointly coat the coating of rice bran fatty alkanol and walnut oil, and the coating shows a more stable storage effect and efficacy in the composition (after storage for 10 months), thereby giving the composition better performance.

[0046] The preparation method of the composition of the present invention is simple, environmentally friendly and has wide application prospects. DETAILED DESCRIPTION

[0047] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are for illustrating the present invention, rather than for limiting the present invention.

[0048] 1. Preparation of coating

[0049] 1. Coating 1: Preparation steps are as follows:

[0050] Step A: In an oil phase pot, heat 3.2 times the mass of rice bran fatty alkanol relative to walnut oil to 88° C. and melt, maintain the temperature, add walnut oil and Tween-80 and Tween-40 in a mass ratio of 1.5:1, and stir at a speed of 500 rpm for 1.5 hours to mix uniformly to obtain an oil phase; wherein the mass ratio of walnut oil to Tween 40 is 1:3.6;

[0051] Step B: Adding tragacanth gum and β-cyclodextrin in a mass ratio of 4:1 and water to an aqueous phase pot, heating to 60°C, and stirring at a speed of 500 rpm for 0.8 hour to mix uniformly to obtain an aqueous phase; wherein the mass ratio of walnut oil to β-cyclodextrin is 1:4, the ratio of the mass of walnut oil to the total mass of tragacanth gum and β-cyclodextrin is 1:20, and the mass ratio of walnut oil to water is 1:85;

[0052] Step C: adding the oil phase to the water phase, stirring at a speed of 500 rpm for 2 hours to mix uniformly, and then spray drying at a temperature of 105° C. to obtain a coating 1 of rice bran fatty alkanol and walnut oil.

[0053] 2. Coating 2: Preparation steps are as follows:

[0054] Step A: In an oil phase pot, heat rice bran fatty alkanol in an amount three times the mass of walnut oil to 87° C. and melt, maintain the temperature, add walnut oil and Tween-80 and Tween-40 in a mass ratio of 1.6:1, and stir at a speed of 550 rpm for 1.2 hours to mix uniformly to obtain an oil phase; wherein the mass ratio of walnut oil to Tween 40 is 1:3.8;

[0055] Step B: Adding tragacanth gum and β-cyclodextrin in a mass ratio of 3.8:1 and water to an aqueous phase pot, heating to 65°C, and stirring at a speed of 550 rpm for 1 hour to mix uniformly to obtain an aqueous phase; wherein the mass ratio of walnut oil to β-cyclodextrin is 1:3.7, and the mass ratio of walnut oil to water is 1:100;

[0056] Step C: adding the oil phase to the water phase, stirring at a speed of 500 rpm for 2.2 hours to mix uniformly, and then spray drying at a temperature of 102° C. to obtain a coating 2 of rice bran fatty alkanol and walnut oil.

[0057] 3. Coating 3: Preparation steps are as follows:

[0058] Step A: In an oil phase pot, heat 3.2 times the mass of rice bran fatty alkanol relative to walnut oil to 88° C. and melt, maintain the temperature, add walnut oil and Tween-80 and Tween-40 in a mass ratio of 1.5:1, and stir at a speed of 500 rpm for 1.5 hours to mix uniformly to obtain an oil phase; wherein the mass ratio of walnut oil to Tween 40 is 1:3.6;

[0059] Step B: Add β-cyclodextrin and water to an aqueous phase pot, heat to 60° C., and stir at 500 rpm for 0.8 hour to mix uniformly to obtain an aqueous phase; wherein the mass ratio of walnut oil to β-cyclodextrin is 1:20, and the mass ratio of walnut oil to water is 1:85;

[0060] Step C: adding the oil phase to the water phase, stirring at a rotation speed of 500 rpm for 2 hours to mix uniformly, and then spray drying at a temperature of 105° C. to obtain a coating 3 of rice bran fatty alkanol and walnut oil.

[0061] 4. Coating 4: Preparation steps are as follows:

[0062] Step A: In an oil phase pot, heat 3.2 times the mass of rice bran fatty alkanol relative to walnut oil to 88° C. and melt, maintain the temperature, add walnut oil and Tween-80 and Tween-40 in a mass ratio of 1.5:1, and stir at a speed of 500 rpm for 1.5 hours to mix uniformly to obtain an oil phase; wherein the mass ratio of walnut oil to Tween 40 is 1:3.6;

[0063] Step B: Add tragacanth gum and water to an aqueous phase pot, heat to 60°C, and stir at 500 rpm for 0.8 hour to mix uniformly to obtain an aqueous phase; wherein the mass ratio of walnut oil to tragacanth gum is 1:20, and the mass ratio of walnut oil to water is 1:85;

[0064] Step C: Add the oil phase to the water phase, stir at a speed of 500 rpm for 2 hours to mix evenly, and then spray dry at a temperature of 105° C. to obtain a coating 4 of rice bran fatty alkanol and walnut oil.

[0065] 5. Coating 5: Preparation steps are as follows:

[0066] Step A: In an oil phase pot, heat 3.2 times the mass of rice bran fatty alkanol relative to walnut oil to 88° C. and melt, maintain the temperature, add walnut oil and Tween-80 and Tween-40 in a mass ratio of 1.5:1, and stir at a speed of 500 rpm for 1.5 hours to mix uniformly to obtain an oil phase; wherein the mass ratio of walnut oil to Tween 40 is 1:3.6;

[0067] Step B: Adding tragacanth gum and β-cyclodextrin in a mass ratio of 1:1 and water to an aqueous phase pot, heating to 60°C, and stirring at 500 rpm for 0.8 hour to mix uniformly to obtain an aqueous phase; wherein the mass ratio of walnut oil to the total mass of tragacanth gum and β-cyclodextrin is 1:20, and the mass ratio of walnut oil to water is 1:85;

[0068] Step C: adding the oil phase to the water phase, stirring at a rotation speed of 500 rpm for 2 hours to mix uniformly, and then spray drying at a temperature of 105° C. to obtain a coating 5 of rice bran fatty alkanol and walnut oil.

[0069] 6. Coating 6: Preparation steps are as follows:

[0070] Step A: In an oil phase pot, heat 3.2 times the mass of rice bran fatty alkanol relative to walnut oil to 88° C. and melt, maintain the temperature, add walnut oil and Tween-80 and Tween-40 in a mass ratio of 1.5:1, and stir at a speed of 500 rpm for 1.5 hours to mix uniformly to obtain an oil phase; wherein the mass ratio of walnut oil to Tween 40 is 1:3.6;

[0071] Step B: Adding tragacanth gum and β-cyclodextrin in a mass ratio of 6:1 and water to an aqueous phase pot, heating to 60°C, and stirring at 500 rpm for 0.8 hour to mix uniformly to obtain an aqueous phase; wherein the mass ratio of walnut oil to the total mass of tragacanth gum and β-cyclodextrin is 1:20, and the mass ratio of walnut oil to water is 1:85;

[0072] Step C: Add the oil phase to the water phase, stir at a speed of 500 rpm for 2 hours to mix evenly, and then spray dry at a temperature of 105° C. to obtain a coating 6 of rice bran fatty alkanol and walnut oil.

[0073] 2. Preparation of Anti-Fatigue Composition

[0074] 1. Composition 1, preparation steps are as follows:

[0075] Step A: Weigh the raw materials according to mass: 1:4 parts of rice bran fatty alkanol and walnut oil coating, 2.5 parts of honeysuckle extract, 2 parts of peony bark extract, 35 parts of whey protein, 22 parts of collagen peptide, 12 parts of crystalline fructose, 1.2 parts of vitamin B1, 0.8 parts of taurine, 7 parts of β-nicotinamide mononucleotide, and 2 parts of wolfberry polysaccharide, wherein the mass ratio of rice bran fatty alkanol and walnut oil coating to wolfberry polysaccharide is 2:1;

[0076] Step B: adding the coating of rice bran fatty alkanol and walnut oil, honeysuckle extract, peony bark extract, whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, β-nicotinamide mononucleotide, and wolfberry polysaccharide into a mixer and mixing to obtain the composition 1.

[0077] 2. Composition 2: The preparation steps are as follows:

[0078] Step A: Weigh the raw materials according to mass: 2:4.1 parts of rice bran fatty alkanol and walnut oil coating, 2.4 parts of honeysuckle extract, 2.1 parts of peony bark extract, 34 parts of whey protein, 24 parts of collagen peptide, 11 parts of crystalline fructose, 1.1 parts of vitamin B1, 0.9 parts of taurine, 6.9 parts of β-nicotinamide mononucleotide, and 1.8 parts of wolfberry polysaccharide, wherein the mass ratio of the rice bran fatty alkanol and walnut oil coating to wolfberry polysaccharide is 2.28:1;

[0079] Step B: Add the rice bran fatty alkyl alcohol and walnut oil coating 2, honeysuckle extract, peony bark extract, whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, β-nicotinamide mononucleotide, and wolfberry polysaccharide into a mixer and mix to obtain the composition 2.

[0080] 3. Composition 3: The preparation steps are as follows:

[0081] Step A: Weigh the following raw materials by mass: honeysuckle extract: 2.5 parts, peony bark extract: 2 parts, whey protein: 35 parts, collagen peptide: 22 parts, crystalline fructose: 12 parts, vitamin B1: 1.2 parts, taurine: 0.8 parts, β-nicotinamide mononucleotide: 7 parts, and wolfberry polysaccharide: 6 parts;

[0082] Step B: Add the rice bran fatty alkyl alcohol and walnut oil coating 1, honeysuckle extract, peony bark extract, whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, β-nicotinamide mononucleotide, and wolfberry polysaccharide into a mixer and mix to obtain the composition 3.

[0083] 4. Composition 4: The preparation steps are as follows:

[0084] Step A: Weigh the following raw materials by mass: 1:6 parts of rice bran fatty alkanol and walnut oil coating, 2.5 parts of honeysuckle extract, 2 parts of peony bark extract, 35 parts of whey protein, 22 parts of collagen peptide, 12 parts of crystalline fructose, 1.2 parts of vitamin B1, 0.8 parts of taurine, and 7 parts of β-nicotinamide mononucleotide;

[0085] Step B: Add the rice bran fatty alkyl alcohol and walnut oil coating 1, honeysuckle extract, peony bark extract, whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, β-nicotinamide mononucleotide, and wolfberry polysaccharide into a mixer and mix to obtain the composition 4.

[0086] 5. Composition 5: The preparation steps are as follows:

[0087] Step A: Weigh the raw materials according to mass: 1:1 part of the coating of rice bran fatty alkanol and walnut oil, 2.5 parts of honeysuckle extract, 2 parts of peony bark extract, 35 parts of whey protein, 22 parts of collagen peptide, 12 parts of crystalline fructose, 1.2 parts of vitamin B1, 0.8 parts of taurine, 7 parts of β-nicotinamide mononucleotide, and 5 parts of wolfberry polysaccharide, wherein the mass ratio of the coating of rice bran fatty alkanol and walnut oil to wolfberry polysaccharide is 0.2:1;

[0088] Step B: Add the rice bran fatty alkyl alcohol and walnut oil coating 1, honeysuckle extract, peony bark extract, whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, β-nicotinamide mononucleotide, and wolfberry polysaccharide into a mixer and mix to obtain the composition 5.

[0089] 6. Composition 6: The preparation steps are as follows:

[0090] Step A: Weigh the raw materials according to mass: 1:5 parts of rice bran fatty alkanol and walnut oil coating, 2.5 parts of honeysuckle extract, 2 parts of peony bark extract, 35 parts of whey protein, 22 parts of collagen peptide, 12 parts of crystalline fructose, 1.2 parts of vitamin B1, 0.8 parts of taurine, 7 parts of β-nicotinamide mononucleotide, and 1 part of wolfberry polysaccharide, wherein the mass ratio of rice bran fatty alkanol and walnut oil coating to wolfberry polysaccharide is 5:1;

[0091] Step B: Add the rice bran fatty alkyl alcohol and walnut oil coating 1, honeysuckle extract, peony bark extract, whey protein, collagen peptide, crystalline fructose, vitamin B1, taurine, β-nicotinamide mononucleotide, and wolfberry polysaccharide into a mixer and mix to obtain the composition 6.

[0092] 7. Composition 7: The coating 3 of rice bran fatty alkanol and walnut oil is used to replace the coating 1 of rice bran fatty alkanol and walnut oil in composition 1. Other components are the same as those in composition 1.

[0093] 8. Composition 8: The coating 4 of rice bran fatty alkanol and walnut oil is used to replace the coating 1 of rice bran fatty alkanol and walnut oil in composition 1. Other aspects are the same as those of composition 1.

[0094] 9. Composition 9: The coating 5 of rice bran fatty alkanol and walnut oil is used to replace the coating 1 of rice bran fatty alkanol and walnut oil in composition 1. Other components are the same as those in composition 1.

[0095] 10. Composition 10: The coating 6 of rice bran fatty alkanol and walnut oil is used to replace the coating 1 of rice bran fatty alkanol and walnut oil in composition 1. Other components are the same as those in composition 1.

[0096] 2. Effect Test

[0097] After the above compositions 1-10 were stored at 30° C. for 10 months, the following performance tests were performed.

[0098] Test samples: Compositions 1-10 after storage for 10 months, and Red Bull beverage.

[0099] Grouping of test subjects: Several male mice weighing approximately 20-22 g were selected; after feeding the mice for 1 week, the mice were randomly divided into 12 groups: a blank control group (normal saline group), the above-mentioned composition 1-10 groups, and a Red Bull beverage group, with 15 mice in each group.

[0100] Administration: The above composition groups 1-10 and the Red Bull beverage group were gavaged at a dose of 2.2 g / kg / d, and the blank control group was given an equal mass of normal saline. Each group was gavaged once a day for 20 consecutive days.

[0101] Experimental testing:

[0102] 1. Mouse weighted swimming test: 40 minutes after the last drug administration, weights were placed on the tails of mice, with the weight being 15% of the mouse's own weight. The mice were placed in a constant temperature swimming pool with a water temperature of (25±1)°C. The timer was started after each group of mice were placed in the pool. The time it took for the mice to sink to the surface of the water for 10 seconds and then fail to surface was recorded as the exhaustive swimming time. The average exhaustive swimming time t value of each group was then calculated.

[0103] 2. Blood Lactate, Serum Urea Nitrogen, and Muscle Glycogen Content Assays: After the mice in the above groups finished swimming, blood was collected from their eye sockets and serum was separated. The mice were sacrificed, and hind leg muscles were harvested. Serum samples were assayed for blood lactate (A), serum urea nitrogen (B), and muscle glycogen levels (C). The mean values ​​for blood lactate (A), serum urea nitrogen (B), and muscle glycogen levels (C) were calculated for each group.

[0104] Among them, the average exhaustive swimming time t value test results of each group are shown in Table 1.

[0105] Table 1: Effect of exhaustive swimming time t

[0106] Test Group t / s Test Group t / s Blank control group 156.51 Composition 5 groups 309.08 Red Bull Drink Group 205.29 Composition 6 groups 317.13 Composition 1 set 327.38 Composition 7 groups 307.53 Composition 2 groups 324.45 Composition 8 groups 316.67 Composition 3 groups 300.73 Composition 9 groups 310.32 Composition 4 groups 314.25 Composition 10 groups 321.16

[0107] From the exhaustive swimming time t effect test results in Table 1, it can be seen that after the anti-fatigue composition obtained by the present invention was stored for 10 months, the mouse weight-bearing swimming experiment showed that the exhaustive swimming time t value of the mouse was as high as 327.38 seconds.

[0108] The anti-fatigue composition containing both rice bran fatty alkanols and walnut oil coatings and wolfberry polysaccharides showed a longer t-value for exhaustive swimming in mice, as shown in the comparison between Group 1 and Groups 3-4. Furthermore, when the mass ratio of the rice bran fatty alkanols and walnut oil coating to wolfberry polysaccharides was (1-2):1, the composition had a more effective anti-fatigue effect.

[0109] The present invention uses tragacanth gum and β-cyclodextrin to co-coat a coating of rice bran fatty alkanol and walnut oil. The coating exhibits more stable storage and efficacy in the composition (after 10 months of storage). The co-coating of the two significantly enhances the anti-fatigue effect of the rice bran fatty alkanol and walnut oil coating, as shown in the comparison of Groups 1, 7, and 8. A ratio of (3-5):1 demonstrates more stable performance and better anti-fatigue effect.

[0110] The test results of the average blood lactate value A, the average serum urea nitrogen value B, and the average muscle glycogen level C of each group of serum samples are shown in Table 2.

[0111] Table 2: Blood lactate (A), serum urea nitrogen (B), and muscle glycogen level (C) tests

[0112] Test Group A value / mmol / L B value / mmol / L C value / mg / g Blank control group 14.75 28.01 22.82 Red Bull Drink Group 11.86 21.94 28.62 Composition 1 set 5.73 11.27 38.54 Composition 2 groups 5.86 11.53 38.07 Composition 3 groups 6.90 13.59 34.31 Composition 4 groups 6.28 12.38 36.50 Composition 5 groups 6.51 12.81 35.72 Composition 6 groups 6.17 12.13 36.96 Composition 7 groups 6.61 12.99 35.41 Composition 8 groups 6.21 12.23 36.83 Composition 9 groups 6.47 12.74 35.87 Composition 10 groups 5.98 11.78 37.64

[0113] As shown in Table 2, the blood lactate (A) value, serum urea nitrogen (B) value, and muscle glycogen level (C) value test results indicate that after 10 months of storage, the anti-fatigue composition obtained by the present invention had an average serum lactate (A) value, an average serum urea nitrogen (B) value, and an average muscle glycogen level (C) value of 5.73 mmol / L, 11.27 mmol / L, and 38.54 mg / g, respectively. The anti-fatigue composition containing both rice bran fatty alkanols and walnut oil and wolfberry polysaccharides exhibited more favorable serum lactate (A) values, serum urea nitrogen (B) values, and muscle glycogen level (C) values. Tragacanth gum and β-cyclodextrin co-coated the rice bran fatty alkanols and walnut oil coating, which exhibited a more stable storage effect and efficacy in the composition (after 10 months of storage), imparting better anti-fatigue performance to the composition.

[0114] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. An anti-fatigue composition comprising rice bran fatty alkanol and plant components, characterized in that: The raw materials for the preparation include, by weight: 1.5-5 parts of a coating of rice bran fatty alkanol and walnut oil, 0.8-4.5 parts of honeysuckle extract, 1-5 parts of peony bark extract, 30-40 parts of whey protein, 10-25 parts of collagen peptide, 8-22 parts of crystalline fructose, 0.3-2 parts of vitamin B1, 0.2-1.5 parts of taurine, 4-10 parts of β-nicotinamide mononucleotide, and 1-3 parts of wolfberry polysaccharide; The preparation method of the coating of rice bran fatty alkanol and walnut oil comprises the following steps: Step A: Heat and melt rice bran fatty alkanol, add walnut oil, Tween-80 and Tween-40, mix well to obtain an oil phase; Step B: heating and mixing tragacanth gum, β-cyclodextrin and water to obtain an aqueous phase; Step C: The oil phase is added to the aqueous phase, mixed uniformly, and spray dried to obtain a coating of rice bran fatty alkanol and walnut oil; The mass ratio of tragacanth gum to β-cyclodextrin is (3-5): 1; The mass ratio of the rice bran fatty alkanol and walnut oil coating to wolfberry polysaccharide is (1-2):

1.

2. The anti-fatigue composition according to claim 1, wherein: The mass ratio of the rice bran fatty alkanol to walnut oil is (2.5-4):

1.

3. The anti-fatigue composition according to claim 1, wherein: The amount of the rice bran fatty alkanol and walnut oil coating is 2-4.8 parts.

4. The method for preparing an anti-fatigue composition comprising a rice bran fatty alkanol and a plant component as claimed in any one of claims 1 to 3, wherein: It involves weighing the raw materials and mixing them in a mixer.

5. The preparation method according to claim 4, wherein: Also includes the packaging step.

6. An application, characterized in that: The invention comprises using the anti-fatigue composition according to any one of claims 1 to 3 in preparing an anti-fatigue preparation.