A mild and non-irritating preservative composition and its application in cosmetics

Abstract

The present application provides a mild and non-irritating preservative composition, which comprises plant preservatives composed of peony root bark extract, Japanese cypress extract, lemon water extract, black bean water extract, etc., and ingredients selected from p-hydroxyacetophenone, 1,2-hexanediol, raspberry ketone, pentylene glycol, caprylyl glycol, ethylhexylglycerin, etc. The preservative composition of the present application is green, safe, and low in irritation, and can be used in various types of cosmetics.

Description

Technical field:

[0001] The present application belongs to the field of cosmetics, preservatives and plant extracts. Specifically, the present application provides a mild and non-irritating preservative composition and its application in cosmetics. Background technology:

[0002] Products in the fields of cosmetics, medicine, daily chemicals, etc. need to consider the issue of preservation. In addition to adopting appropriate sterilization, packaging, and preservation methods, the use of preservatives is also essential for most products. Currently, a large number of chemical preservatives are widely used in the above fields. These preservatives have deficiencies in terms of safety, irritation, broad-spectrum antibacterial properties, and consumer sensory perception.

[0003] A large number of plant extracts have antibacterial and preservative effects. The plant antibacterial mechanism is extensive (such as destroying the biofilm structure, inhibiting the activity of specific enzymes, etc.), and the safety, versatility and consumer sensory impression are good. The use of plant extracts or their compositions as preservatives suitable for the preparation of cosmetics and other fields has a good prospect. Summary of the invention:

[0004] In order to fully develop the antiseptic and antibacterial effects of plant extracts, the applicant has tried a large number of combinations of plant extracts to provide an antiseptic composition that has both antibacterial effects and irritation.

[0005] On the one hand, the present application provides a mild and non-irritating preservative composition, which includes a plant preservative, which is an aqueous solution or 1,3-butylene glycol solution containing 5-15% w / v peony root bark extract, 8-15% w / v Japanese cypress extract, 2-6% w / v lemonade extract, and 2-6% w / v black soybean water extract.

[0006] Furthermore, the antiseptic composition also contains one or more of p-hydroxyacetophenone, 1,2-hexanediol, raspberry ketone, pentylene glycol, caprylyl glycol, and ethylhexylglycerin.

[0007] Furthermore, the plant preservative is a 1,3-butylene glycol solution containing 12% w / v peony root bark extract, 10% w / v Japanese cypress extract, 5% w / v lemonade extract, 5% w / v black bean water extract and 20-40% w / v seaweed extract.

[0008] Furthermore, the plant preservative is a 1,3-butylene glycol solution containing 12% w / v peony root bark extract, 10% w / v Japanese cypress extract, 5% w / v lemonade extract, 5% w / v black bean water extract and 30% w / v seaweed extract.

[0009] Furthermore, the seaweed extract is an enzyme-modified Polysiphonia extract.

[0010] Furthermore, the seaweed extract is a Polysiphonia extract modified by bromelain or papain and aminopeptidase.

[0011] Furthermore, the seaweed extract is prepared by the following method: dissolving the water extract of Polysiphonia lanuginosa in water containing 200-400U / mL bromelain and 200-400U / mL aminopeptidase at 15-25 times the mass, adjusting the pH value to 6.5-7.5, performing enzyme treatment at 50-60 degrees Celsius for 1.5-4 hours; inactivating the enzyme for 5 minutes; and concentrating by rotary evaporation and freeze-drying to obtain the enzyme-modified seaweed extract.

[0012] Furthermore, the seaweed extract is prepared by the following method: dissolving the water extract of Polysiphonia lanuginosa in 20 times the mass of water containing 300U / mL bromelain and 300U / mL aminopeptidase, adjusting the pH value to 6.5-7.5, and performing enzyme treatment at 50-60 degrees Celsius for 2 hours; inactivating the enzyme for 5 minutes; and concentrating by rotary evaporation and freeze-drying to obtain the enzyme-modified seaweed extract.

[0013] In another aspect, the present application provides use of the preservative composition in preparing cosmetics.

[0014] Furthermore, the cosmetic is a facial cleanser, facial mud, facial cream, eye cream, toner or essence.

[0015] The use of the antiseptic composition of the present application is not limited to the above-mentioned cosmetics, and other dosage forms of cosmetics can be used as long as there is no physical or chemical incompatibility between the dosage form or ingredients and the ingredients in the composition. After appropriate adjustment and verification, the antiseptic composition of the present application can also be used in other medical and daily chemical products. Description of the drawings:

[0016] Figure 1 The figure shows the chicken embryo chorioallantoic membrane test results of formula 9-12 (the negative control is 0.9% saline, and the positive control is 1% NaOH).

[0017] Figure 2 This is a 28-day antiseptic challenge test report for the facial cleanser solution (as a finished product, its ingredients are slightly different from those of Example 3).

[0018] Figure 3 This is a 28-day anti-corrosion challenge test report of the mud film solution (as a finished product, its ingredients are slightly different from the solution in Example 3). Specific implementation method:

[0019] Example 1 Main raw materials and methods

[0020] Peony root bark and Japanese cypress extracts are made by the applicant:

[0021] Extraction method of moutan bark root bark: take moutan bark root bark, crush it and then sieve it to obtain moutan bark root bark fine powder; take the sieved moutan bark root bark fine powder and add it into a Soxhlet extractor, use 5 times w / v n-hexane as solvent, and reflux extraction at a temperature of 70-80°C for 24 hours; use a rotary evaporator to reduce pressure and concentrate the extract to obtain moutan bark root bark extract (oily).

[0022] Extraction method of Japanese cypress: Take Japanese cypress leaves, cut them into pieces and put them into a steam distillation extraction device, add 3 times w / v water, and extract by steam distillation for 8 hours; collect the oily part to obtain Japanese cypress extract (oily).

[0023] Lemon water extract (10:1) and black bean water extract (20:1) were customized from third-party manufacturers.

[0024] The water extract of Polysiphonium (polysaccharide content 50%), the water extract of Kelp (polysaccharide content 65%), and the water extract of Sargassum (polysaccharide content 50%) are customized from third-party manufacturers, and the raw materials used are Polysiphonium, Sargassum, and Kelp.

[0025] α-Amylase, cellulase, pectinase, dextranase, papain, bromelain, and aminopeptidase were purchased from Xiasheng, Dongheng Huadao and other manufacturers, and all were food grade.

[0026] The Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Burkholderia cepacia ATCC 25416, Enterobacter japonensis ATCC 33028, Candida albicans ATCC 10231, Aspergillus niger ATCC 16404, and Penicillium glabrata ATCC 10464 used in the antibacterial experiment are all commonly used standard strains in the field and were purchased from an agent of ATCC.

[0027] The enzyme treatment method of the seaweed extract is as follows: dissolving the seaweed extract in 20 times the mass of pure water containing a predetermined amount of enzyme, adjusting the pH value (α-amylase, cellulase, pectinase, dextranase are adjusted to 6.0, and papain, bromelain, and aminopeptidase are adjusted to 7.0), performing enzyme treatment at 55 degrees Celsius for 2 hours; inactivating the enzyme in a 90-degree Celsius water bath for 5 minutes; and concentrating by rotary evaporation and freeze-drying to obtain the enzyme-modified seaweed extract.

[0028] The experimental procedures and evaluation standards for chicken embryo chorioallantoic membrane refer to the method of SNT 2329-2009. The experiment is carried out using a 5% aqueous solution of a plant preservative. ES≤2 means no irritation; 3≤ES≤8 means mild irritation; 8≤ES≤14 means moderate irritation; 14≤ES≤20 means strong irritation / corrosiveness.

[0029] Example 2: Using seaweed extract to alleviate the irritation of plant preservatives

[0030] The antibacterial effects of single ingredients and combinations such as peony root bark, cinnamon bark extract, and Japanese cypress extract have been confirmed in the prior art (see the applicant's previous applications CN20211066369.4, CN20211066369.8, etc.). The applicant further studied a plant preservative composed of peony root bark extract, Japanese cypress extract, lemonade extract, and black bean water extract, which is composed of 12% w / v peony root bark extract, 10% w / v Japanese cypress extract, 5% w / v lemonade extract, and 5% w / v water extract dissolved in 1,3-butanediol.

[0031] The extract has good antibacterial effect, but it is found in practice that it performs poorly in terms of irritation. Therefore, the applicant tried to introduce 30% w / v seaweed extract or enzyme-modified seaweed extract to solve the irritation problem, forming the following formula:

[0032] Table 1 Plant preservatives introduced into 30% w / v seaweed extract and their ES values ​​of the chicken embryo chorioallantoic membrane test endpoint

[0033]

[0034] Table 2 Plant preservatives introduced into enzyme-modified seaweed extracts and their ES values ​​in the chicken embryo chorioallantoic membrane test endpoint

[0035]

[0036]

[0037] As shown in Tables 1 and 2, although polysaccharides are the main components of seaweed extracts, neither the polysaccharides themselves nor the polysaccharide hydrolysates can significantly alleviate the irritation of the above-mentioned plant preservatives. Instead, the protease-treated Polysiphonium extract showed a certain mitigation effect. From the perspective of deep hydrolysis, the applicant tried to use two proteases in combination with aminopeptidase. Table 3 Plant preservatives of seaweed extracts modified by the introduction of composite enzymes and their ES values ​​in the chicken embryo chorioallantoic membrane test endpoint

[0038]

[0039] The results are shown in Table 3 and Figure 1 As shown in the figure, the protein components in the deep hydrolysis of the Polysiphonium extract effectively reduced the irritation of the plant preservatives. It is speculated that this may be due to the hydrolysis of the Polysiphonium extract (such as phycobiliprotein) to obtain certain soothing and disease-resistant active peptide components. Formula 11 still achieved a non-irritating effect when the actual dosage was 10 times higher.

[0040] Example 3 Practical application of plant preservatives in cosmetics

[0041] The following products were prepared using the plant preservative of Formulation 11:

[0042] 1. An amino acid cleansing formula that is non-irritating to the eyes, prepared from the following raw materials in percentage by weight: 25-30% glycerol, 25-30% potassium cocoyl glycinate, 3-5% acrylic acid (ester) copolymer, 1.0-1.5% glyceryl stearate citrate, 0.5% p-hydroxyacetophenone, 0.4% VMC (plant preservative), 0.5% 1,2-hexanediol, and the balance is water.

[0043] 2. A mild and non-irritating cleansing mud mask formula, prepared from the following raw materials in percentage by weight: 2% stearic acid polyether-21, 1% stearic acid polyether-2, 5% caprylic / capric triglyceride, 2.0% cetearyl alcohol, 2% hydrogenated polydecene, 1% squalane, 0.1% tocopheryl acetate, 15% kaolin, 5% bentonite, 5% butylene glycol, 5% glycerol, 0.05% disodium EDTA, 0.05% potassium cetyl phosphate, 0.3% xanthan gum, 0.5% papain, 0.35% raspberry ketone, 0.3% VMC (plant preservative), 0.5% 1,2-hexanediol, and the balance is water.

[0044] 3. A minimalist formula of an aqueous solution with moisturizing properties that can be applied to sensitive skin, which is prepared from the following raw materials in percentage by weight: 3% glycerol, 4% butylene glycol, 0.1% sodium hyaluronate, 0.08% carbomer, 0.08% arginine, 2% betaine, 2% trehalose, 2% pentylene glycol, 0.5% 1,2-hexanediol, 0.2% caprylyl glycol, 0.05% ethylhexylglycerin, and the balance is water.

[0045] 4. A minimalist water-based formula having oil-control properties that is acceptable to eyes for sensitive skin, which is prepared from the following raw materials in percentage by weight: 3% glycerin, 4% butylene glycol, 0.05% sodium hyaluronate, 0.08% carbomer, 5% witch hazel flower water, 0.05% zinc gluconate, 0.08% arginine, 3% pentylene glycol, 0.5% VMC (plant preservative), 0.5% hexylene glycol, 0.05% ethylhexylglycerin, and the balance is water.

[0046] 5. A minimalist formula for an eye cream with anti-wrinkle and firming properties that is suitable for sensitive skin, the cream being prepared from the following raw materials in percentage by weight: 2% polyglyceryl-3 methyl glucose distearate, 5% caprylic / capric triglyceride, 1.0% cetearyl alcohol, 3% polydimethylsiloxane, 2% hydrogenated polydecene, 1% squalane, 0.1% tocopheryl acetate, 5% butylene glycol, 5% glycerol, 0.05% disodium EDTA, 0.05% potassium cetyl phosphate, 0.5% acrylates / C10-30 alkyl acrylate crosspolymer, 1% hydroxypropyl tetrahydropyrantriol, 0.4% VMC (plant preservative), 0.2% p-hydroxyacetophenone, 0.2% 1,2-hexanediol, and the balance being water.

[0047] The above products were subjected to a 28-day antiseptic challenge, and the results are shown in Table 4. Figure 2-3 As shown:

[0048] Table 4 28-day antiseptic challenge results of actual products

[0049]

[0050]

[0051] The results show that the antiseptic composition of the present application has good antibacterial effect in various types of cosmetics.

Claims

1. A mild and non-irritating antiseptic composition, characterized in that: The antiseptic composition comprises a plant preservative, which is a 1,3-butylene glycol solution containing 12% w / v of peony root bark extract, 10% w / v of Japanese cypress extract, 5% w / v of lemon water extract, 5% w / v of black soybean water extract and 20-40% w / v of seaweed extract; The seaweed extract is prepared by the following method: dissolving the water extract of Polysiphonia lanuginosa in water containing 200-400U / mL bromelain and 200-400U / mL aminopeptidase at 15-25 times the mass, adjusting the pH value to 6.5-7.5, performing enzyme treatment at 50-60 degrees Celsius for 1.5-4 hours; inactivating the enzyme for 5 minutes; and concentrating by rotary evaporation and freeze-drying to obtain the seaweed extract.

2. The antiseptic composition according to claim 1, further comprising one or more of p-hydroxyacetophenone, 1,2-hexanediol, raspberry ketone, pentylene glycol, caprylyl glycol, and ethylhexylglycerin.

3. The preservative composition according to claim 1 or 2, wherein the plant preservative is a 1,3-butylene glycol solution containing 12% w / v peony root bark extract, 10% w / v Japanese cypress extract, 5% w / v lemonade extract, 5% w / v black soybean water extract and 30% w / v seaweed extract.

4. The preservative composition according to claim 1, wherein the seaweed extract is prepared by the following method: dissolving the water extract of Polysiphonia lanuginosa in 20 times the mass of water containing 300U / mL bromelain and 300U / mL aminopeptidase, adjusting the pH value to 6.5-7.5, and subjecting to enzyme treatment at 50-60 degrees Celsius for 2 hours; inactivating the enzyme for 5 minutes; and concentrating by rotary evaporation and freeze-drying to obtain the seaweed extract.

5. Use of the antiseptic composition according to any one of claims 1 to 4 in the preparation of cosmetics.

6. The use according to claim 5, wherein the cosmetic is a cleanser, cleansing mud, facial cream, eye cream, toner or essence.

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