A composition for protecting egg of green lacewing and its application

Abstract

The application discloses a kind of compositions for protecting egg of green lacewing natural enemy insects and application, it is related to agricultural technology field.The composition is by following weight fraction component composition: p-umbelliferone 1-35 parts, ascaridol 1-35 parts, eucalyptol 0.5-10 parts, alpha-pinene 0.5-10 parts, methyl benzoate 0.5-10 parts.The application prospect of the composition for protecting egg of green lacewing natural enemy insects is wide, and preparation method is simple, in the actual application in field, can large-scale utilize green lacewing to control pests, especially for the most, the most widely distributed, the most miscellaneous, the most serious thrips pests effect is good, economic, social and ecological benefits are remarkable.

Description

Technical Field

[0001] This invention relates to the field of agricultural technology, and more specifically, to a composition and its application for protecting the eggs of lacewing-like natural enemy insects. Background Technology

[0002] Lacewings are recognized as predatory natural enemies of insects, possessing large appetites and a wide food range, and have long been effective in controlling pests such as thrips, aphids, whiteflies, and mites. Common species in my country include the large lacewing, the beautiful lacewing (small lacewing), the Chinese lacewing, the leaf-colored lacewing, and the Asian and African lacewings. The volatiles released by plants induced by the feeding behavior of herbivorous insects are highly specific; parasitic wasps utilize this specificity to search for specific hosts, even hosts of different instars. Some plant volatiles attract the natural enemies of pests, while others repel them. The influence of individual volatiles on pest behavior is related to the type and concentration of the volatile; different concentrations and ratios of plant volatiles can even have diametrically opposed effects on insect behavior. Therefore, different plant volatiles have varying degrees of influence on the behavior of natural enemy insects. As a commercially available natural enemy, the lacewing's control role in actual production mainly relies on field release. However, this is hampered by several factors. Firstly, lacewing larvae are cannibalistic, and both adults and larvae feed on the eggs of their own species. Secondly, lacewing eggs are also consumed by other insects. These cannibalistic tendencies and the problem of lacewings being preyed upon by other insects pose significant obstacles to the successful release of lacewings in the field and the sustained maintenance of a high egg population for hatching. Developing a method to prevent lacewing eggs from being eaten before hatching is crucial for the field control of lacewings, aphids, whiteflies, and other pests.

[0003] Therefore, providing a composition for protecting the eggs of lacewing-like natural enemy insects is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0004] In view of this, the present invention provides a composition and application for protecting the eggs of lacewing natural enemy insects, which utilizes the repulsion of lacewing adults, larvae and other pests to plant-derived compounds to protect lacewing eggs from being eaten by lacewing adults, larvae or other pests.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A composition for protecting the eggs of lacewing-like natural enemy insects, the composition comprising the following components in parts by weight: 1-35 parts of p-cymene, 1-35 parts of pyrimethamine, 0.5-10 parts of eucalyptol, 0.5-10 parts of α-pinene, and 0.5-10 parts of methyl benzoate.

[0007] Furthermore, the composition comprises the following components in parts by weight: 5-30 parts of p-cymene, 1-28 parts of pyruvicin, 1-8 parts of eucalyptol, 1-8 parts of α-pinene, and 1-8 parts of methyl benzoate.

[0008] Furthermore, the composition comprises the following components in parts by weight: 8-25 parts of p-cymene, 5-20 parts of pyruvicin, 2-7 parts of eucalyptol, 2-7 parts of α-pinene, and 2-7 parts of methyl benzoate.

[0009] A compositional microemulsion for protecting the eggs of lacewing-like natural enemy insects, said microemulsion comprising the following components in parts by weight:

[0010] Active ingredients: 1-35 parts of p-cymene, 1-35 parts of baicalin, 0.5-10 parts of eucalyptol, 0.5-10 parts of α-pinene, and 0.5-10 parts of methyl benzoate;

[0011] Microemulsion adjuvants: sodium ascorbate 0.05-0.6 parts, glycerin 3-8 parts, sodium diisooctyl succinate sulfonate 2-10 parts, potassium sorbate 0.5-0.8 parts, guar gum 0.3-0.8 parts, emulsifier 10-15 parts;

[0012] 5-60 parts water.

[0013] Furthermore, the preparation method includes the following steps:

[0014] (1) Mix p-cymene, ascaridin, eucalyptol, α-pinene and methyl benzoate in a certain proportion and stir evenly to obtain an active ingredient mixture.

[0015] (2) Mix water and microemulsion additives in a certain proportion and stir evenly to obtain microemulsion additive mixture;

[0016] (3) The active ingredient mixture and the microemulsion adjuvant mixture are thoroughly mixed to obtain a microemulsion of the composition that protects the eggs of lacewing natural enemy insects.

[0017] A composition microcapsule formulation for protecting the eggs of lacewing-like natural enemy insects, said microcapsule formulation comprising the following components in parts by weight:

[0018] Active ingredients: 1-35 parts of p-cymene, 1-35 parts of baicalin, 0.5-10 parts of eucalyptol, 0.5-10 parts of α-pinene, and 0.5-10 parts of methyl benzoate.

[0019] Microencapsulation adjuvants: 8-30 parts toluene diisocyanate, 12-32 parts polyvinyl alcohol, 0.05-0.8 parts glycerol, 0.05-0.6 parts sodium ascorbate, and 0.02-0.5 parts guar gum;

[0020] 5-60 parts water.

[0021] Furthermore, the preparation method includes the following steps:

[0022] 1) Add the active ingredients to a container containing toluene diisocyanate and stir until well mixed. Then add polyvinyl alcohol and stir until well mixed. Finally, add glycerol, stir and let stand.

[0023] 2) Add sodium ascorbate, guar gum and water, filter through 60 mesh to obtain homogenized components, put the attractant microcapsules into an ultra-low temperature freezer and pre-freeze at -80℃, then take them out and dry them in a freeze dryer at -60℃ for 12 hours.

[0024] The application of a composition for protecting the eggs of lacewing natural enemy insects in pest control, wherein the lacewing is any one of the following: large lacewing, small lacewing, Chinese lacewing, leaf-colored lacewing, and Asian-African lacewing.

[0025] As can be seen from the above technical solution, compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] This invention contains a large number of screened plant-derived odor compounds that repel adult and larval lacewings, as well as other predatory insects, without adversely affecting the lacewing eggs themselves; in fact, they may even accelerate hatching. This allows the lacewing eggs to be protected from predation when released in the field. The composition is characterized by low cost, environmental friendliness, high efficiency, strong protective effect, and precise control of target pests. This composition for protecting the eggs of lacewing-like natural enemies has broad application prospects, a simple preparation method, and can be used on a large scale in the field for pest control, particularly effective against thrips, which are the most diverse, widely distributed, have the most varied host systems, and cause the most serious damage. The economic, social, and ecological benefits are significant. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0028] Figure 1 To investigate the protective effect of different concentrations of lacewing-protecting insect egg compositions on lacewing eggs in the presence of adult lacewings, an asterisk (*) indicates a significant difference between treatments and controls.

[0029] Figure 2 To investigate the protective effect of different concentrations of lacewing-protecting insect egg compositions on lacewing eggs in the presence of 3rd instar larvae, an asterisk (*) indicates a significant difference between treatments and controls.

[0030] Figure 3 To show the protective effect of different concentrations of lacewing-protecting insect egg compositions on lacewing eggs in the presence of adult lacewings, an asterisk (*) indicates a significant difference between treatments and controls.

[0031] Figure 4 To evaluate the protective effect of different concentrations of lacewing-protective insect egg compositions on lacewing eggs in the presence of 3rd instar larvae, an asterisk (*) indicates a significant difference between treatments and controls.

[0032] Figure 5 Example 2 illustrates the protective effect of a microemulsion composition for protecting the eggs of lacewing natural enemies on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control.

[0033] Figure 6 Example 3 illustrates the protective effect of a microemulsion composition for protecting the eggs of lacewing natural enemies on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control.

[0034] Figure 7 Example 4 illustrates the protective effect of a microemulsion composition for protecting the eggs of lacewing natural enemies on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control.

[0035] Figure 8 Example 5 illustrates the protective effect of a microemulsion composition for protecting the eggs of lacewing natural enemies on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control.

[0036] Figure 9 Example 6 illustrates the protective effect of microcapsules containing lacewing-like natural enemy insect eggs on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control.

[0037] Figure 10 Example 7 illustrates the protective effect of microcapsules containing lacewing-like natural enemy insect eggs on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control.

[0038] Figure 11 Example 8 illustrates the protective effect of microcapsules containing lacewing-like natural enemy insect eggs on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control.

[0039] Figure 12Example 9 illustrates the protective effect of microcapsules containing lacewing-like natural enemy insect eggs on lacewing eggs when releasing lacewing eggs, larvae, and adults in the field. An asterisk (*) indicates a significant difference between the treatment and the control. Detailed Implementation

[0040] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0041] Example 1

[0042] A Y-shaped olfactory instrument was used to determine the directional behavioral response of large lacewings to the composition (the Y-shaped olfactory instrument used was improved based on the basic principle of olfactory instruments using authorized patent number ZL201020239767.7). The main arm of the Y-shaped tube was 20cm long, and each of the two arms was 10cm long, with the included angle between the two arms being 90°. A distilled water bottle and an activated carbon bottle were sequentially connected to the ends of the two arms using silicone tubing. Finally, the activated carbon bottle was left open to the atmosphere, and the insect release bottle was connected to a suction device with gauze. Filter paper strips were cut into 5cm × 1cm pieces, folded into a "V" shape, and placed in the glass tube at the trap entrance.

[0043] Tilt the Y-shaped olfactory instrument at 30° and control the temperature at 25±2℃. Take 100μl of the mixture of the five effective components of this invention (mass ratio of p-cymene: cytotoxicin: eucalyptol: α-pinene: methyl benzoate = 3:3:1:1:1), the mixture of methyl benzoate and p-cymene (mass ratio of methyl benzoate: p-cymene = 1:5), and each individual component, and evenly drop them onto a filter paper strip and place it in one of the flavor source bottles. Use a filter paper with 100μl of liquid paraffin on the other side as a reference. The airflow rate is 1L / min. The airflow entering the two tubes is filtered through activated carbon and humidified with distilled water.

[0044] Thirty adult lacewings were placed in insect release bottles each time, and the tests were repeated three times. After each test, the inner and outer walls of the Y-shaped tube were wiped with anhydrous ethanol using a cotton ball and then dried. The two arms of the Y-shaped tube were then swapped with the flavor source bottle and the control to eliminate the possible influence of geometric errors of the two arms on the lacewing behavior. The test insects were placed in the insect release bottles of the Y-shaped tube to begin the experiment. Each test insect crawled past 1 / 2 of the selection arm and stayed for more than 10 seconds to be considered to have made a selection. Lacewings that stayed on the main arm of the Y-shaped tube were considered to have no response. The experimental results within 30 minutes were recorded and the avoidance rate was calculated according to the following formula.

[0045] Repellency rate / % = (Number of worms in the control tube - Number of worms in the treatment tube) / Total number of worms × 100.

[0046] The results showed that the repellency rates of the effective component mixture of the present invention, the mixture of methyl benzoate and p-cymene, and p-cymene, ascaridin, eucalyptol, α-pinene, and methyl benzoate alone against large lacewings were 88.89%, 52.22%, 66.67%, 64.44%, 58.89%, 54.44%, and 51.11%, respectively. This indicates that the repellency effect of the repellent of the present invention is significantly higher than that of the mixture of methyl benzoate and p-cymene, p-cymene, ascaridin, eucalyptol, α-pinene, and methyl benzoate.

[0047] The results above show that the composition of this invention has a significant repellent effect on adult lacewings. Similar repellent effects were also observed in indoor behavioral selection experiments on predatory natural enemies such as adult and larvae of *Lysimachia sinensis*, *Lysimachia clethroides*, *Lysimachia florida*, and *Lysimachia africana*.

[0048] Example 2

[0049] A microemulsion composition for protecting the eggs of lacewing natural enemies, comprising the following components by weight: 1 part p-cymene, 15 parts pyruvicin, 5 parts eucalyptol, 3 parts α-pinene, 2 parts methyl benzoate, 0.05 parts sodium ascorbate, 3 parts sodium diisooctyl succinate sulfonate, 4 parts glycerin, 0.5 parts potassium sorbate, 0.5 parts guar gum, 11 parts emulsifier, and 54.95 parts water.

[0050] The preparation steps are as follows:

[0051] (1) The active ingredients (p-cymene, ascaridin, eucalyptol, α-pinene, methyl benzoate) are mixed and stirred and dissolved at room temperature to obtain the oil phase;

[0052] (2) Mix water and all additives thoroughly;

[0053] (3) Slowly add (1) to (2), stir, shear and emulsify, take a sample, test and obtain the result.

[0054] Example 3

[0055] A microemulsion composition for protecting the eggs of lacewing natural enemies, comprising, by weight, 35 parts of p-cymene, 5 parts of pyruvic acid, 2 parts of eucalyptol, 2 parts of α-pinene, 2 parts of methyl benzoate, 0.05 parts of sodium ascorbate, 3 parts of sodium diisooctyl succinate sulfonate, 4 parts of glycerin, 0.5 parts of potassium sorbate, 0.5 parts of guar gum, 11 parts of emulsifier, and 34.95 parts of water.

[0056] The preparation method is the same as in Example 2.

[0057] Example 4

[0058] A microemulsion composition for protecting the eggs of lacewing natural enemy insects, comprising the following components by weight: 14 parts p-cymene, 6 parts pyruvicin, 3 parts eucalyptol, 2 parts α-pinene, 1 part methyl benzoate, 0.05 parts sodium ascorbate, 3 parts sodium diisooctyl succinate sulfonate, 4 parts glycerin, 0.5 parts potassium sorbate, 0.5 parts guar gum, 11 parts emulsifier, and 54.95 parts water.

[0059] The preparation method is the same as in Example 2.

[0060] Example 5

[0061] A microemulsion composition for protecting the eggs of lacewing natural enemy insects, comprising the following components by weight percentage: 12 parts p-cymene, 5.5 parts pyruvicin, 3.5 parts eucalyptol, 3.3 parts α-pinene, 1.7 parts methyl benzoate, 0.06 parts sodium ascorbate, 3.2 parts sodium diisooctyl succinate sulfonate, 3.8 parts glycerin, 0.55 parts potassium sorbate, 0.4 parts guar gum, 10 parts emulsifier, and 55.99 parts water.

[0062] The preparation method is the same as in Example 2.

[0063] Example 6

[0064] A microcapsule formulation for protecting the eggs of lacewing natural enemy insects, comprising the following components by weight: 1 part p-cymene, 30 parts pyruvicin, 5 parts eucalyptol, 6 parts α-pinene, 4 parts methyl benzoate, 0.05 parts sodium ascorbate; 19 parts polyvinyl alcohol, 21 parts toluene diisocyanate, 0.05 parts glycerol, 0.1 parts guar gum, and 13.8 parts water.

[0065] The mixed active ingredients were added to a container containing toluene diisocyanate and stirred. Then polyvinyl alcohol was added and stirred. Glycerol was added and stirred and allowed to stand. Other additives and water were added, filtered to obtain a homogenized component, dried, and microcapsules were obtained.

[0066] Example 7

[0067] A microcapsule formulation for protecting the eggs of lacewing natural enemy insects, comprising the following components by weight: 35 parts p-cymene, 4 parts pyruvicin, 3 parts eucalyptol, 3 parts α-pinene, 3 parts methyl benzoate, 0.07 parts sodium ascorbate; 24 parts polyvinyl alcohol, 16 parts toluene diisocyanate, 0.03 parts glycerol, 0.1 parts guar gum, and 11.8 parts water.

[0068] The preparation method is the same as in Example 6.

[0069] Example 8

[0070] A microcapsule formulation for protecting the eggs of lacewing natural enemy insects, comprising, by weight, 22 parts of p-cymene, 16.5 parts of pyruvic acid, 5.5 parts of eucalyptol, 6.3 parts of α-pinene, 4 parts of methyl benzoate, 0.06 parts of sodium ascorbate; 21 parts of polyvinyl alcohol, 19 parts of toluene diisocyanate, 0.06 parts of glycerol, 0.1 parts of guar gum, and 5.48 parts of water.

[0071] The preparation method is the same as in Example 6.

[0072] Example 9

[0073] A microcapsule formulation for protecting the eggs of lacewing natural enemy insects, comprising the following components by weight: 24 parts p-cymene, 18 parts pyruvicin, 6.2 parts eucalyptol, 6.8 parts α-pinene, 5 parts methyl benzoate, 0.05 parts sodium ascorbate; 17 parts polyvinyl alcohol, 16 parts toluene diisocyanate, 0.08 parts glycerol, 0.08 parts guar gum, and 6.79 parts water.

[0074] The preparation method is the same as in Example 6.

[0075] The composition prepared in Example 2 was used to spray lacewing eggs at dilutions of 6000 and 9000 times. Two adult lacewings were placed in each test dish, and the remaining egg count was recorded on days 1, 2, and 3 to calculate the protection rate. The experiment was repeated four times. Lacewing egg masses treated with water were used as a control.

[0076] The composition prepared in Example 3 was used to spray lacewing eggs at dilutions of 6000 and 9000 times. Two third-instar lacewing larvae were placed in each experimental dish, and the remaining egg count was recorded on days 1, 2, and 3 to calculate the protection rate. The experiment was repeated four times. Lacewing egg masses treated with water were used as a control.

[0077] The composition prepared in Example 4 was used to spray *Lysimachia clethroides* eggs at dilutions of 6000 and 9000 times. Two adult *Lysimachia clethroides* were placed in each test dish, and the remaining egg count was recorded on days 1, 2, and 3 to calculate the protection rate. The experiment was repeated four times. *Lysimachia clethroides* egg masses treated with water were used as a control.

[0078] The composition prepared in Example 5 was used to spray *Lysimachia foetida* eggs at dilutions of 6000 and 9000 times. Two third-instar larvae of *Lysimachia foetida* were placed in each experimental dish, and the remaining egg count was recorded on days 1, 2, and 3 to calculate the protection rate. The experiment was repeated four times. *Lysimachia foetida* egg masses treated with water were used as a control.

[0079] from Figure 1 , Figure 2 , Figure 3 , Figure 4The survey data shows that the protection rates of *Leptochloa macrocarpa* and *Leptochloa serpentina* eggs in the control area were significantly lower than those in the composition-treated area. Before hatching, the composition protecting the eggs of lacewing predators effectively protected *Leptochloa macrocarpa* eggs from predation by predatory insects. In addition to *Leptochloa macrocarpa* and *Leptochloa serpentina*, Examples 2-5 also showed similar protective effects on the eggs of *Leptochloa serpentina*, *Leptochloa sinensis*, and *Leptochloa africana*.

[0080] Microemulsions containing protective lacewing-like natural enemy insect eggs, prepared in Examples 2, 3, 4, and 5, were used. Two relatively independent strawberry fields, more than 500 meters apart, each 15 meters long and 5 meters wide, were selected. At the early stage of thrips infestation, 50 egg masses of large lacewings treated with the protective lacewing-like natural enemy insect eggs were placed, along with 50 adult large lacewings and 50 third-instar larvae in each treatment. Another egg mass containing large lacewings treated with water served as a control. The number of large lacewing eggs in each treatment was investigated and recorded 1, 2, and 3 days after application to calculate the protection rate. The treatment was repeated three times.

[0081] from Figure 5-8 The survey data shows that, 1, 2, and 3 days after treatment, the protection rate of lacewing eggs in the control area was significantly lower than that in the treated area, indicating a significant protective effect on lacewing eggs. Specifically, the microemulsion composition prepared in Example 4 showed the best protective effect on the eggs of lacewing-like natural enemy insects.

[0082] Microencapsulation formulations of compositions for protecting the eggs of lacewing-like natural enemy insects, prepared in Examples 6, 7, 8, and 9, were used. Two 300m² plots of similar growth were selected. 2 In strawberry fields, isolation zones were established around the perimeter. At the early stage of thrips infestation, 60 treated egg masses were mixed with 100 adult lacewings and 3rd instar larvae and released. Lacewing eggs in the control area were not treated. The number of lacewing eggs at each location was investigated and recorded 1, 2, and 3 days after application to calculate the protection rate. The experiment was repeated 3 times.

[0083] from Figure 9-12 The survey data shows that, 1, 2, and 3 days after treatment, the protection rate of lacewing eggs in the control area was significantly lower than that in the treated area, indicating a significant protective effect on lacewing eggs. Specifically, the microcapsules prepared in Example 9 showed the best protective effect on the eggs of lacewing predators.

[0084] When the above-mentioned composition for protecting the eggs of lacewing natural enemies is used for field release, it can protect the lacewing eggs from being eaten by adult lacewings, larvae, and other predatory natural enemies.

[0085] Toxicity test (the test insects were from the Nanjing Academy of Agricultural Sciences)

[0086] The microcapsule formulation of the composition for protecting the eggs of lacewing natural enemy insects prepared in Example 8 was used. Freshly laid lacewing eggs were used, with 200 capsules each for the control and treatment groups, and the experiment was conducted at 25°C.+ At 1℃ and 70% relative humidity, 95% of lacewing eggs in the control area hatched into larvae in 103 hours. In the treatment area, with the compound of this invention suspended, all lacewing eggs hatched into larvae in 81 hours. This indicates that exposure of lacewing eggs to the composition of this invention for protecting the eggs of natural enemies of lacewings has no adverse effect on egg development; on the contrary, it promotes the hatching rate. Utilizing these plant-derived volatiles as the main components is a relatively ideal, simple, and effective method for protecting lacewing eggs.

[0087] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composition for protecting the eggs of lacewing-like natural enemy insects, characterized in that, The composition comprises the following components in parts by weight: 1-35 parts of p-cymene, 1-35 parts of baicalin, 0.5-10 parts of eucalyptol, 0.5-10 parts of α-pinene, and 0.5-10 parts of methyl benzoate.

2. The composition for protecting the eggs of lacewing natural enemy insects according to claim 1, characterized in that, The composition comprises the following components in parts by weight: 5-30 parts of p-cymene, 1-28 parts of cytotoxicin, 1-8 parts of eucalyptol, 1-8 parts of α-pinene, and 1-8 parts of methyl benzoate.

3. The composition for protecting the eggs of lacewing natural enemy insects according to claim 1, characterized in that, The composition comprises the following components in parts by weight: 8-25 parts of p-cymene, 5-20 parts of baicalin, 2-7 parts of eucalyptol, 2-7 parts of α-pinene, and 2-7 parts of methyl benzoate.

4. A microemulsion composition for protecting the eggs of lacewing-like natural enemy insects, characterized in that, The microemulsion comprises the following components in parts by weight: Active ingredients: 1-35 parts of p-cymene, 1-35 parts of baicalin, 0.5-10 parts of eucalyptol, 0.5-10 parts of α-pinene, and 0.5-10 parts of methyl benzoate; Microemulsion adjuvants: sodium ascorbate 0.05-0.6 parts, glycerin 3-8 parts, sodium diisooctyl succinate sulfonate 2-10 parts, potassium sorbate 0.5-0.8 parts, guar gum 0.3-0.8 parts, emulsifier 10-15 parts; 5-60 parts water.

5. The microemulsion composition for protecting the eggs of lacewing natural enemy insects according to claim 4, characterized in that, The preparation method includes the following steps: (1) Mix p-cymene, ascaridin, eucalyptol, α-pinene and methyl benzoate in a certain proportion and stir evenly to obtain an active ingredient mixture; (2) Mix water and microemulsion additives in a certain proportion and stir evenly to obtain microemulsion additive mixture; (3) The active ingredient mixture and the microemulsion adjuvant mixture are thoroughly mixed to obtain a microemulsion of the composition that protects the eggs of lacewing natural enemy insects.

6. A microcapsule formulation of a composition for protecting the eggs of lacewing-like natural enemy insects, characterized in that, The microcapsule formulation comprises the following components in parts by weight: Active ingredients: 1-35 parts of p-cymene, 1-35 parts of baicalin, 0.5-10 parts of eucalyptol, 0.5-10 parts of α-pinene, and 0.5-10 parts of methyl benzoate. Microencapsulation adjuvants: 8-30 parts toluene diisocyanate, 12-32 parts polyvinyl alcohol, 0.05-0.8 parts glycerol, 0.05-0.6 parts sodium ascorbate, and 0.02-0.5 parts guar gum; 5-60 parts water.

7. The microcapsule formulation of the composition for protecting the eggs of lacewing natural enemy insects according to claim 6, characterized in that, The preparation method includes the following steps: 1) Add the active ingredients to a container containing toluene diisocyanate and stir until well mixed. Then add polyvinyl alcohol and stir until well mixed. Finally, add glycerol, stir and let stand. 2) Add sodium ascorbate, guar gum and water, filter through 60 mesh to obtain homogenized components, place the components in an ultra-low temperature freezer at -80℃ for pre-freezing, and then take them out and place them in a freeze dryer at -60℃ for 12 hours.

8. The application of the composition for protecting the eggs of lacewing natural enemy insects as described in any one of claims 1-3 in pest control, characterized in that, The lacewing is any one of the following: large lacewing, beautiful lacewing, Chinese lacewing, leaf-colored lacewing, and Asian-African lacewing.

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