Use of torreya grandis inner seed coat extract in preparing a product for treating constipation

Abstract

The present application provides the application of torreya grandis inner seed coat extract in preparing a product for treating constipation, wherein the torreya grandis inner seed coat extract is obtained by stripping the black coat of torreya grandis after roasting, and then extracting the black coat with water or 50% ethanol, and drying the extract obtained by vacuum concentration; and the prepared torreya grandis inner seed coat extract is used in preparing various dosage forms of drugs for treating constipation and products for lubricating intestines and defecating. The prepared torreya grandis inner seed coat extract powder has the effect of promoting intestinal propulsion, can improve the small intestinal propulsion function, and can achieve good lubricating intestines and defecating effect, and can be used for treating constipation. The torreya grandis inner seed coat powder can be used for preparing food with the effect of lubricating intestines and defecating, so that the function of treating and conditioning constipation can be achieved through diet therapy, and the side effects of the commonly used drugs such as magnesium sulfate, castor oil, cisapride, loperamide, domperidone and bisacodyl can be overcome, and the resource utilization rate of torreya grandis is increased, and the economic benefits are increased.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological medicine, and particularly relates to application of Torreya grandis inner seed coat extract in preparation of a product for treating constipation. BACKGROUND

[0002] Torreya grandis, also known as Torreya grandis, is a plant of Torreya grandis in Taxaceae. The characteristic of Torreya grandis after picking is green olive-shaped green fruit. Torreya grandis green fruit is composed of perisperm (exotesta), shell, endosperm and seed kernel from outside to inside. Torreya grandis is one of the rare dried fruits in the world, which has special structure, unique flavor and high nutritional value and medicinal function. Torreya grandis endosperm is edible, but it is often discarded because of bitter taste. A large amount of endosperm waste is also produced in the process of roasting Torreya grandis. Current studies have shown that Torreya grandis endosperm has certain antioxidant effect. However, there is no detailed literature and patent report on the role and preparation method of Torreya grandis in treating constipation.

[0003] Constipation is a common clinical symptom, which is a common functional gastrointestinal disease mainly characterized by reduced defecation frequency, difficulty in defecation and dry stool. It occurs worldwide, has high incidence, complex causes, usually reduces intestinal movement time and prolongs fecal discharge time, and causes harmful bacteria to accumulate in the colon, thereby affecting intestinal health. Intestinal diseases, mental stress factors, living habits, environmental changes and the like can cause intestinal function disorder and lead to constipation. With the acceleration of modern social rhythm, the change of living habits and the increase of mental stress, the incidence of constipation is increasing, and gradually becomes one of the important diseases that disturb people's healthy life.

[0004] At present, the most commonly used drugs for treating constipation include magnesium sulfate, castor oil, cisapride, trimebutine maleate, domperidone and bisacodyl, etc. These drugs have good therapeutic effect on constipation, but also have many adverse reactions such as headache, rash, drowsiness, abdominal pain, diarrhea, electrolyte disturbance, etc. Long-term use will cause certain damage to the gastrointestinal tract, cause great harm to the body, and produce dependence. Therefore, it is of great social value and economic value to develop a product with safety and effectiveness for the prevention or treatment of constipation. Food therapy has the advantages of high safety and good therapeutic effect, and food therapy for treating constipation is more and more applied at present.

[0005] Torreya grandis is one of the economic crops in Zhejiang Province, and its main component is oil, more than 20 kinds of aromatic compounds, and part of protein and various vitamins. According to the 2020 edition of Chinese Pharmacopoeia, the function and treatment of Torreya grandis are as follows: killing insects and reducing accumulation, moistening the lungs and stopping coughing, moistening dryness and relieving constipation. It is used for hookworm disease, ascariasis, tapeworm disease, abdominal pain due to accumulation of worms, infantile malnutrition, lung dry cough, and constipation. Modern research on Torreya grandis is mostly on Torreya grandis kernel. The inner seed coat of Torreya grandis involved in the patent is the black pericarp of Torreya grandis nut, which is slightly bitter and usually scraped off and discarded when eaten. However, there is no detailed report on the effect of Torreya grandis inner seed coat and Torreya grandis on moistening the intestines and relieving constipation. SUMMARY

[0006] The application provides the application of Torreya grandis inner seed coat extract in the preparation of a product for treating constipation, so as to solve the defects in the prior art.

[0007] The application provides the application of Torreya grandis inner seed coat extract in the preparation of a product for treating constipation, and the Torreya grandis inner seed coat extract refers to the black skin of Torreya grandis after post-ripening treatment and frying, which is obtained by water or 50% ethanol reflux extraction and vacuum concentration and drying.

[0008] According to the application, the Torreya grandis inner seed coat extract is obtained by adopting the conventional pile ripening and frying technology of Torreya grandis, that is, the mature Torreya grandis is treated by post-ripening and pile ripening, the false seed coat is peeled off in time after the false seed coat rots, the pile ripening is continued until the inner seed coat of Torreya grandis changes from purple red to black brown, then the natural water washing and airing are performed; the first high-heat frying is performed, the black kernel skin in the fruit core is separated by cold water, the second frying is performed, and the black kernel skin is peeled and scraped; the obtained Torreya grandis inner seed coat is added with 10 times the amount of distilled water or 50% ethanol solution for reflux decocting three times, and the combined filtrate is cooled to obtain the Torreya grandis inner seed coat extract, which can also be spray dried or vacuum concentrated and dried to obtain a powder.

[0009] According to the application, the Torreya grandis inner seed coat extract is used to prepare food, health products or medicines.

[0010] According to the application, the dosage form of the Torreya grandis inner seed coat extract medicine is any one of oral liquid, oral tablet, capsule, granule and powder.

[0011] The application provides an application of a torreya grandis inner seed coat extract in preparation of a product for treating constipation, and the prepared torreya grandis inner seed coat powder extract has a promoting effect on intestinal propulsion, can improve small intestinal propulsion function, can achieve a good effect of lubricating intestines and defecation, and can be used for treating constipation. In addition, torreya grandis is a medicine and food homologous traditional Chinese medicinal material approved by the Ministry of Health, has the advantages of safety, almost no side effect and being edible, can be used for food production and processing, and the torreya grandis inner seed coat powder is used for preparing food with the effects of lubricating intestines and defecation, so that the function of treating and conditioning constipation is achieved through diet therapy, side effects of commonly used existing drugs such as magnesium sulfate, castor oil, cisapride, loperamide, domperidone and bisacodyl are overcome, the resource utilization rate of torreya grandis is increased, and economic benefits are increased. BRIEF DESCRIPTION OF DRAWINGS

[0012] In order to more clearly illustrate the technical solutions of the present application or prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0013] Figure 1 is a schematic diagram of the influence of different parts of torreya grandis on defecation of mice provided by the embodiment of the present application;

[0014] Figure 2 is a picture of colon tissue pathological section and HE staining of mice in each group with different treatments provided by the embodiment of the present application. DETAILED DESCRIPTION

[0015] In order to make the purpose, technical scheme and advantages of the present application more clear, the technical scheme of the present application will be described clearly and completely in the following with reference to the drawings in the present application. Obviously, the described embodiments are some embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0016] Embodiment 1

[0017] Preparation method of torreya grandis inner seed coat extract: take torreya grandis collected from Xinxianhe, Tonglu, and after ripening, when the aril rots, remove the aril in time, continue to pile for about 15 days until the inner seed coat of torreya grandis turns from purple red to black brown, then wash with natural water, and dry. First, roast with strong fire, then soak in cold water or salt water for about 1 minute, and roast again. Shell the roasted torreya grandis, take out the kernel, and separate the black coat. The obtained torreya grandis inner seed coat is added with 10 times the amount of distilled water or 50% ethanol solution for reflux decoction 3 times, and the filtrate is obtained after cooling. The filtrate can also be spray dried or vacuum concentrated and dried to obtain a powder, thereby obtaining the torreya grandis inner seed coat extract.

[0018] Test example

[0019] Study on the effect of different parts of torreya grandis on constipation of mice

[0020] (1) Preparation of related reagents

[0021] Torreya grandis oil solution: the kernel obtained in Example 1 is used to extract oil at 200°C, and the extracted torreya grandis oil is centrifuged at 13000 rpm and 25°C for 5 min, and the upper clear oil layer is taken for standby.

[0022] Torreya grandis residue powder solution: after the oil extraction residue is slightly cooled, it is ground into powder, sieved, and then mixed with distilled water to prepare a torreya grandis residue powder solution with a mass concentration of 100 mg / ml. Shake well before use.

[0023] Torreya grandis inner seed coat powder solution: the black coat obtained in Example 1 is ground into powder and sieved. After sieving, 10 times the volume of distilled water is added, and reflux decoction is performed 3 times. The filtrate is obtained after cooling. The administration dose is 300 mg / kg of black coat powder, and administration is performed.

[0024] 5% ink: weigh 5 g of gum arabic, add water and boil until the solution is transparent, then add 2.5 g of activated carbon powder and boil 3 times. After cooling, make up to 50 ml and store at 4°C. Shake well before use.

[0025] 1 mg / ml loperamide hydrochloride solution: weigh 10 mg of loperamide hydrochloride, dissolve in pure water, and make up to 10 ml.

[0026] (2) Experimental animals

[0027] 60 SPF KM male mice, weighing 30-35 g, were raised in the clean level animal room of Zhejiang Chinese Medicine University, with temperature of 25°C and humidity of 40%±5%. The light and dark cycle was 12 h. All mice were adaptively fed for one week before the experiment, with free access to water and food.

[0028] (3) Establishment of functional constipation model of mice

[0029] The mice functional constipation model was established by inhibiting the gastrointestinal peristalsis of mice to reduce defecation by giving loperamide hydrochloride modeling drug. After the mice were given the corresponding drugs and 5% ink by gavage, the first black stool time and the amount of black stool within 5h of the mice were observed to evaluate the effect of the drugs on improving constipation in mice

[0030] (4) Animal grouping and dosing

[0031] After the mice were adaptively fed for one week, the body weight of the mice was weighed in turn, and 60 mice were randomly divided into blank group, model group, positive drug group (castor oil), torreya grandis oil group, heiyi powder group, and torreya grandis residue powder group according to the body weight (bw), 10 mice in each group. The blank group and the model group were given distilled water (0.2 ml / day) by gavage, the positive drug group was given castor oil (0.1 ml / day) by gavage, the torreya grandis oil group was given torreya grandis oil (0.2 ml / day) by gavage, the heiyi powder group was given heiyi powder solution (300 mg / kg bw) by gavage, and the torreya grandis residue powder group was given torreya grandis residue powder solution (600 mg / kg bw) by gavage.

[0032] (5) Defecation observation experiment of mice

[0033] After 3 days of gavage of the corresponding drugs, the mice were fasted for 16 hours without water, and the mice functional constipation model was induced by gavage of loperamide 10 mg / kg in each group. After 30 minutes, the drug group was given the daily gavage amount of drugs and 0.2 ml of blank ink, and the normal group and the model group were given the daily gavage amount of distilled water and 0.2 ml of blank ink. The first black stool time and the amount of black stool within 5h of each mouse were recorded.

[0034] (6) Small intestine propulsion rate experiment of mice

[0035] After 3 days of gavage of the corresponding drugs, the mice were fasted for 16 hours without water, and the mice functional constipation model was induced by gavage of loperamide 10 mg / kg in each group. After 30 minutes, the drug group was given the daily gavage amount of drugs and 0.2 ml of blank ink, and the normal group and the model group were given the daily gavage amount of distilled water and 0.2 ml of blank ink. The mice were sacrificed by cervical dislocation 30 minutes after the ink was given, and the intestinal tract from the pylorus to the cecum was quickly removed, straightened without changing the length of the intestinal tract, and the ink propulsion rate was calculated.

[0036] Ink propulsion rate = L1 / L0 x 100%

[0037] In the formula, L1 (cm) is the ink propulsion length, and L0 (cm) is the length from the pylorus to the cecum.

[0038] (7) HE staining observation experiment

[0039] Take out 4% paraformaldehyde fixed mouse colon tissue, slice deparaffinization, gradient ethanol immersion dehydration, hematoxylin staining for 5 min, PBS washing, 1% hydrochloric acid ethanol differentiation, ammonia water counterstain for 5 s, eosin solution staining for 30 s, gradient ethanol immersion dehydration, xylene transparency, neutral glue sealing section, observe the intestinal barrier pathological changes of colon tissue under optical microscope.

[0040] The results of the effects of different parts of torreya grandis on defecation of mice are shown in Table 1 and Figure 1 Compared with the blank group, the first black stool time of the model group mice was prolonged, the 5h black stool particle number was reduced, the small intestine propulsion rate was significantly reduced, the colon section villi atrophy was adhered, the number of goblet cells was significantly reduced, a small amount of inflammatory cells infiltration was visible in the mucosa layer, the muscle layer was thinned, which indicated that the modeling was successful. Compared with the model group, the torreya grandis inner seed coat powder extract group could significantly shorten the first black stool time, increase the black stool particle number and small intestine propulsion rate (P<0.01). The results of HE staining pathological observation found that the colon villi of the torreya grandis inner seed coat powder extract group remained relatively complete, the colon goblet cells were relatively normal, and the muscle layer remained complete. The effects of different parts of torreya grandis on intestinal moistening and defecation of mice, and the comprehensive results of HE staining pathological observation showed that the torreya grandis inner seed coat powder extract had the best effect.

[0041] Table 1 Effects of different parts of torreya grandis on the first black stool time, 5h black stool particle number and ink propulsion rate of mice

[0042]

[0043]

[0044] Note: Compared with the blank group, #P<0.05, ##P<0.01, ###P<0.001; compared with the model, *P<0.05, **P<0.01, ***P<0.001.

[0045] The torreya grandis inner seed coat powder extract has the effects of shortening the first black stool time, increasing the black stool particle number, improving the small intestine propulsion function of mice, reducing the colon inflammatory cells and the like, so as to achieve a good intestinal moistening and defecation effect, and can be used for treating constipation. The torreya grandis inner seed coat powder can be used for treating constipation and used for preparing intestinal moistening and defecation products. In particular, torreya grandis is a medicine and food homologous traditional Chinese medicinal material approved by the Ministry of Health, has the advantages of high safety, small side effect and edible, can be used for food production, and therefore the torreya grandis inner seed coat powder can be used for preparing food with intestinal moistening and defecation function, so as to play a function of treating and conditioning constipation through diet therapy, and overcome the side effects of the commonly used existing drugs such as magnesium sulfate, castor oil, cisapride, loperamide, domperidone and bisacodyl.

[0046] It should be pointed out finally that the above embodiments are only used to illustrate the technical solutions of the present application, but not to limit the same; and although the present application has been described in detail with reference to the foregoing embodiments, it should be appreciated by those skilled in the art that the technical solutions recorded in the foregoing embodiments can be modified, or some technical features thereof can be replaced equivalently; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. The application of Torreya grandis inner seed coat extract in the preparation of products for treating constipation, characterized in that, The method for obtaining the inner seed coat extract of Torreya grandis is as follows: mature Torreya grandis are harvested and subjected to post-ripening composting. After the aril rots, the aril is promptly removed, and the plant continues to be piled until the inner seed coat of Torreya grandis turns from purplish-red to dark brown. Then, the plant is washed with natural water and dried. The plant is then stir-fried over high heat for the first time, and the black kernel skin in the kernel is separated by cold water. The plant is then stir-fried again, and the black kernel skin is scraped off. The obtained inner seed coat of Torreya grandis is added to 10 times the amount of distilled water or 50% ethanol solution and refluxed and boiled 3 times. The filtrates are combined and cooled to obtain the final product. Alternatively, it can be spray-dried or vacuum concentrated and dried to obtain powder.

2. The application of the Torreya grandis inner seed coat extract according to claim 1 in the preparation of a product for treating constipation, characterized in that, The inner seed coat extract of Torreya grandis is used to manufacture pharmaceuticals.

3. The application of the Torreya grandis inner seed coat extract according to claim 1 in the preparation of a product for treating constipation, characterized in that, The dosage form of the Torreya grandis inner seed coat extract is any one of oral liquid, oral tablets, capsules, granules, or powder.

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