A method for breeding a new variety of red crayfish

Abstract

The application discloses a breeding method of a new variety of red chirochirus, and relates to the technical field of breeding of new varieties of shrimps.The breeding steps are as follows: (1) parent cross: male chirochirus of yellow abdomen and female common chirochirus are used as parent shrimps to cross, and F1 generation is produced; (2) first in-family inbreeding: the chirochirus of yellow abdomen in the F1 generation is selected to mate with the chirochirus of yellow abdomen, and F2 generation is produced; (3) second in-family inbreeding: the red chirochirus in the F2 generation is selected to mate with the red chirochirus, and F3 generation is obtained, and the F3 generation is the new variety of the red chirochirus.The new variety of the red chirochirus with red body color can be bred, the new variety of the chirochirus is rich in types, the new variety has good growth performance, and has remarkable commercial traits, and can be popularized for breeding.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of breeding new varieties of shrimps, and particularly relates to a breeding method of a new variety of red red-knuckle crayfish. BACKGROUND

[0002] The red-knuckle crayfish is originally from Oceania and is a large freshwater shrimp introduced from Australia, also known as Australian freshwater lobster. It is one of the best freshwater shrimps in the world. It lives in freshwater all its life, is omnivorous, has a fast growth rate, can naturally reproduce and hatch in freshwater, has a high survival rate and strong disease resistance, weak aggressiveness, low oxygen tolerance, pollution tolerance, strong adaptability, tender meat, delicious taste, high edible rate, long-distance transportation tolerance, fresh and live market, simple artificial breeding equipment, low cost and other advantages. In recent years, the aquaculture industry has developed rapidly.

[0003] The existing red-knuckle crayfish variety has low intraspecific variation, few characteristic new varieties are bred, and there is a lack of commercial varieties with significant differences. The applicant found that a natural mutant with a yellow abdomen (including legs) appeared in the red-knuckle crayfish breeding population, and through mating design using these mutants, a new variety of red-knuckle crayfish with red body color was obtained, providing a population for red-knuckle crayfish breeding. SUMMARY

[0004] In view of the above problems, the present application provides a breeding method of a new variety of red red-knuckle crayfish, which can breed a new variety of red red-knuckle crayfish with red body color. The new variety has good growth performance and can be popularized for breeding. The specific technical scheme is as follows:

[0005] The breeding method of a new variety of red red-knuckle crayfish comprises the following steps:

[0006] (1) Parental crossbreeding: male yellow abdomen red-knuckle crayfish and female ordinary red-knuckle crayfish are used as parent shrimps for crossbreeding to produce F1 generation; the F1 generation contains yellow abdomen shrimps and normal body color shrimps.

[0007] (2) First inbreeding in family: yellow abdomen red-knuckle crayfish in the F1 generation is selected and mated with yellow abdomen red-knuckle crayfish to produce F2 generation; the F2 generation contains red individuals, yellow abdomen individuals and normal color individuals.

[0008] (3) Second inbreeding in family: red red-knuckle crayfish in the F2 generation is selected and mated with red red-knuckle crayfish to obtain F3 generation; the F3 generation has all red body color, and the F3 generation is the new variety of red red-knuckle crayfish.

[0009] Further, in step (1), the parent shrimp selection criteria are: complete limbs, bright carapace, strong body, healthy, and good vitality.

[0010] Further, in step (1), the male Procambarus clarkii has a weight of 75 g or more; and the female Procambarus clarkii has a weight of 50 g or more. Further, in step (1), the specific method of the parent hybridization is that the male Procambarus clarkii and the female Procambarus clarkii are paired in the same pool at a quantity ratio of 1:6, and after 30 days of cultivation, the female Procambarus clarkii carrying eggs is artificially hatched, and the offspring hatched from each female Procambarus clarkii carrying eggs is a full-sib family, and each full-sib family is cultivated for 180 days to obtain the F1 generation of the kind of prawn.

[0011] Further, in step (2), the specific method of the first intra-family inbreeding is that the same amount of male Procambarus clarkii and female Procambarus clarkii are selected from the F1 generation and cultivated in the same pool for intra-family inbreeding, and after 30 days of cultivation, the female Procambarus clarkii carrying eggs is artificially hatched, and the offspring hatched from each female Procambarus clarkii carrying eggs is a full-sib family, and each full-sib family is cultivated for 180 days to obtain the F2 generation of the kind of prawn.

[0012] Further, in step (3), the specific method of the second intra-family inbreeding is that the red male and female individuals in the F2 generation are selected and cultivated in the same pool at a quantity ratio of 1:1 for intra-family inbreeding, and after 30 days of cultivation, the female individuals carrying eggs are artificially hatched to obtain the F3 generation.

[0013] Further, the environmental conditions for the cultivation are that the pool area is 3-8 m2, the water depth is 50-80 cm, the water dissolved oxygen content is controlled to be greater than or equal to 4 mg / L, and the water temperature is controlled to be 25-30 DEG C.

[0014] Further, the feed for the cultivation has a protein content of greater than or equal to 30%, the daily feeding amount accounts for 2%-5% of the body weight, the feed is fed twice a day, the feeding time is from 8:00 to 10:00 in the morning and from 17:00 to 19:00 in the afternoon.

[0015] Compared with the prior art, the present application has the beneficial effects that the present application can breed a new variety of Procambarus clarkii with red body color, enriches the types of the characteristic new variety of Procambarus clarkii, and the new variety has good growth performance and significant commercial traits, and can be popularized for breeding. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description.

[0017] Figure 1 The pedigree chart for breeding the new variety of Procambarus clarkii. DETAILED DESCRIPTION

[0018] The specific embodiments of the present application are described in detail below, but it should be understood that the scope of protection of the present application is not limited by the specific embodiments.

[0019] Example 1

[0020] A breeding method of a new variety of red crayfish, the steps are as follows:

[0021] (1) Parental crossbreeding: male yellow-bellied red crayfish and female common red crayfish are used as parent crayfish for crossbreeding to produce F1 generation; F1 generation contains yellow-bellied crayfish and normal color crayfish, each accounting for 50%. The parent crayfish selection criteria are: complete limbs, bright carapace, strong body, healthy, good vitality.

[0022] Specific measures:

[0023] One male yellow-bellied red crayfish (natural mutant in the breeding population) with a body weight of about 150g is used for same pool cultivation with six female common red crayfish with a body weight of 50-90g, and three female crayfish are obtained for carrying eggs within 30 days, and three full-sibling families (F101, F102, F103) are obtained by artificial incubation, and the number of each family of fry is 75, 218 and 125, respectively. After being cultivated in three 8m2 cement pools for 60 days, the body color is checked, and the proportion of yellow-bellied individuals in the three families is recorded, as shown in Table 1 in detail:

[0024] The above cultivation environment conditions are: water depth 50cm, control water dissolved oxygen content ≥4mg / L, control water temperature 25℃. During cultivation, feed with protein content ≥30%, daily feeding amount accounts for 2% of body weight, feeding twice a day, feeding time is 8:00-10:00 in the morning, 17:00-19:00 in the afternoon.

[0025] Table 1 Proportion of yellow-bellied individuals in each family of F1 generation

[0026]

[0027] (2) Inbreeding in one family: yellow-bellied red crayfish in F1 generation is selected and mated with yellow-bellied red crayfish to produce F2 generation; F2 generation contains red individuals, yellow-bellied individuals, and normal color individuals, wherein red individuals account for about 25%, yellow-bellied individuals account for about 50%, and normal color individuals account for about 25%

[0028] Specific measures:

[0029] Select 20 individuals (10 females and 10 males) of yellow abdomen from each of the families F101, F102 and F103, and carry out inbreeding within the families in three 8 m2 cement pools. Within 30 days, 17 brood females are obtained, including 6 brood females of family F101, 5 brood females of family F102 and 6 brood females of family F103. The above-mentioned brood females are artificially hatched to obtain three F2 inbred lines (F201, F202 and F203) with quantities of 852, 540 and 682, respectively. After cultivation, it is found that the red individuals account for about 25%, the yellow abdomen individuals account for about 50% and the normal color individuals account for about 25% of the offspring (F2). See Table 2 for details:

[0030] The environmental conditions for the above-mentioned cultivation are as follows: the pool area is 8 m2, the water depth is 60 cm, the water dissolved oxygen content is controlled to be greater than or equal to 4 mg / L, and the water temperature is controlled to be 30°C. During the cultivation, the feed protein content is greater than or equal to 30%, the daily feeding amount accounts for 5% of the body weight, the feeding is carried out twice a day, and the feeding time is from 8:00 to 10:00 in the morning and from 17:00 to 19:00 in the afternoon.

[0031] Table 2 Proportion of individuals with different body colors in F2 generation

[0032]

[0033]

[0034] (3) Secondary inbreeding within the family: red red claw crayfish in the F2 generation are mated with red red claw crayfish of the same type to obtain F3 generation, and the body color of the F3 generation is all red, thereby obtaining a new red variety.

[0035] Specific measures:

[0036] Select 60 red individuals (30 females and 30 males) from each of the families F201, F202 and F203, and carry out inbreeding within the families in three 200 m2 pools. Within 30 days, 49 brood females are obtained, including 15 brood females of family F201, 14 brood females of family F202 and 20 brood females of family F203. The above-mentioned brood females are artificially hatched to obtain three F3 inbred lines (F301, F302 and F303). After cultivation, it is found that the red individuals account for 100% of the offspring (F3). See Table 3 for details.

[0037] The environmental conditions for the above-mentioned cultivation are as follows: the water depth is 60 cm, the water dissolved oxygen content is controlled to be greater than or equal to 4 mg / L, and the water temperature is controlled to be 28°C. During the cultivation, the feed protein content is greater than or equal to 30%, the daily feeding amount accounts for 4% of the body weight, the feeding is carried out twice a day, and the feeding time is from 8:00 to 10:00 in the morning and from 17:00 to 19:00 in the afternoon.

[0038] Table 3F proportion of individuals with different body colors in each family of the third generation

[0039]

[0040] Example 2

[0041] Growth performance evaluation of the red Procambarus clarkia new variety:

[0042] The red Procambarus clarkia new variety obtained by the method is mainly used for breeding and eating.

[0043] The growth performance of the red variety is evaluated by using a 200 square meter pool, and the stocking density is 4 tails per square meter; the control group uses normal body color Procambarus clarkia fry, which is mixed with the red variety fry for evaluation, and the results are shown in Table 4:

[0044] Table 4 Growth performance of the red Procambarus clarkia new variety

[0045]

[0046] The red Procambarus clarkia new variety obtained by the method has good growth performance, and the commercial traits are obvious, and can be popularized for breeding.

[0047] The foregoing description of specific exemplary embodiments of the application is intended to be illustrative only and is not intended to limit the application to the precise forms disclosed. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the application. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application and to allow others skilled in the art to understand the application for various exemplary embodiments with various modifications being applicable. The scope of the application is intended to be defined by the claims and their equivalents.

Claims

1. A method for breeding a new variety of red crayfish, characterized in that, The method comprises the following steps: (1) Parental cross: male Procambarus clarkii and female Procambarus clarkii are used as parent crayfishes to produce F1 generation; the male Procambarus clarkii is a natural mutant from a breeding population; (2) First inbreeding in family: the male Procambarus clarkii and the female Procambarus clarkii in F1 generation are mated to produce F2 generation; (3) Second inbreeding in family: the red male Procambarus clarkii and the red female Procambarus clarkii in F2 generation are mated to produce F3 generation; the F3 generation is all red in body color; the F3 generation is the new variety of the red Procambarus clarkii.

2. The method for breeding a new variety of red crayfish according to claim 1, characterized in that, In step (1), the parent crayfish selection criteria are: complete limbs, bright carapace, strong body, healthy, and good vitality.

3. The method for breeding a new variety of red crayfish according to claim 1, characterized in that, In step (1), the weight of the male Procambarus clarkii is more than 75 g; the weight of the female Procambarus clarkii is more than 50 g.

4. The method for breeding a new variety of red crayfish according to claim 1, characterized in that, In step (1), the specific method of the parental cross is: the male Procambarus clarkii and the female Procambarus clarkii are paired in the same pool at a ratio of 1:6, and after 30 days of cultivation, the female Procambarus clarkii carrying eggs is artificially hatched, and the offspring of each female Procambarus clarkii carrying eggs is a full-sibling family; each full-sibling family is cultivated for 180 days to obtain the F1 generation.

5. The method for breeding a new variety of red crayfish according to claim 1, wherein the red crayfish is a new variety of Procambarus clarkia. In step (2), the specific method of the first inbreeding in family is: equal amounts of male and female Procambarus clarkii are selected from the F1 generation to breed in the same pool for inbreeding in family; after 30 days of pairing and cultivation, the female individuals carrying eggs are artificially hatched, and the offspring of each female individual carrying eggs is a full-sibling family; each full-sibling family is cultivated for 180 days to obtain the F2 generation.

6. The method for breeding a new variety of red crayfish according to claim 1, wherein the red crayfish is a new variety of Procambarus clarkia. In step (3), the specific method of the second inbreeding in family is: red male and female individuals in the F2 generation are selected at a ratio of 1:1 to breed in the same pool for inbreeding in family; after 30 days of pairing, the female individuals carrying eggs are artificially hatched to obtain the F3 generation.

7. The method for breeding a new variety of red crayfish according to claim 4, 5 or 6, wherein the red crayfish is a species of Procambarus clarkii. The environmental conditions for cultivation are: a water pool area of 3-8 square meters, a water depth of 50-80 cm, a water dissolved oxygen content of greater than or equal to 4 mg / L, and a water temperature of 25-30 DEG C.

8. The method for breeding a new variety of red crayfish according to claim 4, 5 or 6, wherein the red crayfish is a species of Procambarus clarkii. The feed protein content during cultivation is greater than or equal to 30%, the daily feed amount is 2%-5% of the body weight, the feed is fed twice a day, the feeding time is from 8:00 to 10:00 in the morning and from 17:00 to 19:00 in the afternoon.

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