A composite moxa cigarette containing leaves of Aquilaria sinensis and a preparation method thereof

Abstract

The application relates to the technical field of fumigation and moxibustion materials, and discloses a composite fumigation and moxibustion cigarette containing bai-muxiang leaves and a preparation method thereof. The preparation method specifically comprises the following steps: crushing and screening the mugwort straw, soaking the mugwort straw in a sodium hydroxide solution, stirring after adding polyethylene glycol, cooling to-20 to-35 DEG C, keeping the temperature for 10-30 min, heating to 30-50 DEG C, keeping the temperature for 10-30 min, cooling to-30 to-40 DEG C again, keeping the temperature for 1-2 h, replacing, uniformly stirring after adding bai-muxiang leaves, drying, cutting into filaments to obtain pretreated filaments; drying the cotton linters, heating to 400-500 DEG C under nitrogen protection, calcining for 10-20 min, continuously heating to 700-750 DEG C, calcining for 5-15 min, cooling to room temperature with the furnace, crushing, uniformly mixing with the pretreated filaments, spraying tobacco leaf water extract on the surface of the mixture, air-drying after mixing, spraying a water composite extract of Hippophae rhamnoides L. again, and air-drying after mixing.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of fumigation and moxibustion materials, and particularly relates to a composite fumigation and moxibustion cigarette containing leaf of Aquilaria sinensis and a preparation method thereof. BACKGROUND

[0002] Traditional fumigation and moxibustion utilizes natural diffusion of moxa smoke to the affected part, and the heat of fumigation and moxibustion is not persistent and thorough enough. After long-term contact with fumigation and moxibustion cigarettes, a large amount of volatile substances and tar are generated during combustion, and long-term inhalation of these substances is easy to cause lung disease, pollute the treatment environment, and is not conducive to health and affects the curative effect.

[0003] The leaf of Aquilaria sinensis is a non-medicinal part of Aquilaria sinensis, which contains trace elements necessary for the human body, such as calcium, zinc, iron, manganese and other minerals, and is of great benefit to the elimination of various adverse factors in the body. The alcohol extract of the leaf of Aquilaria sinensis has some effective pharmaceutical effects of agarwood, such as anti-inflammatory, hemostatic, hypoglycemic, laxative, analgesic, anti-tumor activity, anti-brain hypoxia, etc. The extract of the leaf of Aquilaria sinensis has been proved to have no toxicity and can be developed into food and drugs.

[0004] The leaf of Aquilaria sinensis in China is not only rich in resources, but also has significant health care effects because it belongs to a variety of agarwood. Therefore, it is imperative to develop a cigarette containing the leaf of Aquilaria sinensis which not only meets the requirements of fumigation and moxibustion curative effect but also reduces the negative effects of fumigation and moxibustion. SUMMARY

[0005] The present application aims at solving the problems in the prior art and provides a composite fumigation and moxibustion cigarette containing the leaf of Aquilaria sinensis and a preparation method thereof.

[0006] A preparation method of a composite fumigation and moxibustion cigarette containing the leaf of Aquilaria sinensis, comprising the following steps:

[0007] S1, crushing and sieving the straw of wormwood, soaking in a sodium hydroxide solution, standing at room temperature for 20-40 hours, adding polyethylene glycol and stirring for 1-2 hours, cooling to-20 to-35 DEG C, keeping for 10-30 minutes, warming to 30-50 DEG C, keeping for 10-30 minutes, cooling to-30 to-40 DEG C again, keeping for 1-2 hours, replacing with tert-butyl alcohol, adding the leaf of Aquilaria sinensis and stirring uniformly, freeze-drying, and cutting into filaments to obtain pretreated filaments;

[0008] S2, vacuum drying the linter to a constant weight, adding to a tubular furnace, warming to 400-500 DEG C under nitrogen protection, calcining for 10-20 minutes, continuously warming to 700-750 DEG C, calcining for 5-15 minutes, cooling to room temperature with the furnace, crushing, uniformly mixing with the pretreated filaments, spraying tobacco leaf water extract onto the surface, uniformly mixing and air-drying, and then spraying a water composite extract of Hippophae rhamnoides Linn. again, uniformly mixing and air-drying.

[0009] Preferably, the weight ratio of the aqueous extract of the leaves of Aquilaria sinensis, the aqueous extract of tobacco leaves, and the aqueous composite extract of Hippophae rhamnoides is 1:2-5:1-2.

[0010] Preferably, in S1, the mass ratio of the straw of Artemisia vulgaris, polyethylene glycol, and the leaves of Aquilaria sinensis is 1-5:1-2:80-120.

[0011] Preferably, in S1, the concentration of the sodium hydroxide solution is 0.5-1.5 mol / L.

[0012] Preferably, in S1, the molecular weight of the polyethylene glycol is 500-1500.

[0013] Preferably, in S1, the temperature is raised at a rate of 1-5℃ / min to 30-50℃.

[0014] Preferably, in S2, the mass ratio of the cotton linters used and the straw of Artemisia vulgaris used in S1 is 1-5:1-5.

[0015] Preferably, in S2, the temperature for vacuum drying of the cotton linters is 40-60℃.

[0016] Preferably, in S2, the temperature is raised at a rate of 1-3℃ / min to 400-500℃.

[0017] Preferably, in S2, the aqueous extract of tobacco leaves is prepared by the following steps: soaking tobacco leaves in water for 3-5 h, and then filtering to obtain the aqueous extract of tobacco leaves.

[0018] More preferably, the mass ratio of the tobacco leaves to water is 10-30:200-350.

[0019] Preferably, the aqueous composite extract of Hippophae rhamnoides is prepared by the following steps: soaking Hippophae rhamnoides in water for 15-30 h, filtering, and then adding honey and menthol to the filtrate and stirring to obtain the aqueous composite extract of Hippophae rhamnoides.

[0020] More preferably, the mass ratio of Hippophae rhamnoides, water, honey, and menthol is 10-30:200-350:1-3:0.1-1.

[0021] A composite moxa cigarette containing the leaves of Aquilaria sinensis is prepared by the above method. Beneficial effects

[0022] 1. The present application belongs to the traditional Mongolian medicine incense conditioning innovation product, which is prepared from the extracts of Aquilaria sinensis leaves, sea buckthorn and tobacco leaves. The product has the effects of clearing heat and resolving toxins, dispelling wind and dampness, relieving inflammation and pain, relieving cough and reducing phlegm, and degrading tobacco toxins. In addition, the product has excellent effects on rheumatoid arthritis. The smoke is mellow and elegant, and has no peculiar smell. The product can not only meet the preferences of smokers, but also reduce the harm caused by smoking and passive smoking. Therefore, the product has broad market prospects and will produce great social and economic benefits.

[0023] 2. After the alkali treatment of the mugwort straw, the present application is regenerated with polyethylene glycol freeze-thawing. The generated fiber structure is very loose, and is loaded on the surface of the Aquilaria sinensis leaves. The fiber structure has good affinity and high binding strength. The loose fiber structure can promote the full combustion of the Aquilaria sinensis leaves, greatly reducing the content of harmful substances generated by incomplete combustion and greatly reducing the adverse effects on the human body. The present application provides a new recycling scheme for the mugwort straw, avoiding environmental pollution and resource waste caused by burning and composting. The raw materials used are widely available, the cost is low, and the preparation process is simple.

[0024] 3. Since the cotton linters are used as raw materials and are carbonized, a porous hydrophobic structure with a high specific surface area is formed. The highly porous and high specific surface area hydrophobic structure can quickly adsorb the tar generated by combustion, has high adsorption capacity, and causes less tar pollution, further reducing the harm caused by smoke. At the same time, the ignition point of the hydrophobic structure is much higher than the combustion temperature of the Aquilaria sinensis leaves, so the shell will not be ignited during use. The strong adsorption not only adsorbs heat to reduce the body temperature of the human body, but also adsorbs toxic and harmful gases generated by combustion to avoid harm to the user. When the temperature is low at the tail end of the combustion, the previously absorbed heat can be released, thereby moderating the temperature change. The present application has sustained flame resistance, significantly reduces the amount of smoke generated, and has very low smoke content and taste, which is suitable for large-scale popularization and application.

[0025] 4. The present application extracts tobacco and sea buckthorn after soaking, effectively preserves the effective components in tobacco and sea buckthorn through spraying and drying, and releases the low molecular effective components in Aquilaria sinensis leaves and sea buckthorn through flame combustion and high-temperature atomization. The small molecules quickly enter the human body due to their permeability, thereby playing the role of incense conditioning. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 The highest temperature, the lowest temperature and the concentration of PM10 in the smoke during the combustion process of the incense cigarettes obtained in Example 5 and Comparative Examples 1-2 are compared.

[0027] Figure 2 The AI scores of the rats in the model group, the positive drug group, the Example 5 group, the Comparative Example 1 group and the Comparative Example 2 group at different time points are compared.

[0028] Figure 3 The serum TNF-α, IL-1β concentration comparison chart (unit: pg / mL) of the normal group, positive drug group, example 5 group, comparative example 1 group, and comparative example 2 group of rats is shown in the following table.

[0029] Figure 4 The serum IFN-γ, IL-4 concentration comparison chart (unit: pg / mL) of the normal group, positive drug group, example 5 group, comparative example 1 group, and comparative example 2 group of rats is shown in the following table.

[0030] Note: Figure 3 and Figure 4 In the above tables, the data of the model group is not included in the chart because it is too different from the data of the other groups. DETAILED DESCRIPTION

[0031] The application will be further described below in conjunction with specific examples.

[0032] Example 1 A preparation method of a composite moxa cigarette containing leaf of Aquilaria sinensis, comprising the following steps:

[0033] S1, 1 kg of artemisia straw is crushed and passed through a 60-mesh sieve, soaked in 20 kg of a sodium hydroxide solution with a concentration of 1.5 mol / L, and left to stand at room temperature for 20 h. Then, 1 kg of polyethylene glycol 1500 is added, and the mixture is stirred at a speed of 50 r / min for 1 h. The temperature is lowered to -20℃, and the mixture is kept at this temperature for 10 min. The temperature is then raised to 30℃ at a speed of 1℃ / min, and the mixture is kept at this temperature for 10 min. The temperature is again lowered to -30℃, and the mixture is kept at this temperature for 1 h. Tert-butyl alcohol is used for replacement, and 80 kg of leaf of Aquilaria sinensis is added and stirred uniformly. The mixture is freeze-dried, cut into thin filaments with a width of 0.3±0.1 cm and a length of 0.5±0.1 cm, and the pretreated filaments are obtained.

[0034] S2, 1 kg of cotton linters is dried to a constant weight under vacuum at a temperature of 40℃. The dried cotton linters are added to a tubular furnace, and the temperature is raised to 400℃ at a speed of 1℃ / min under nitrogen protection. The mixture is calcined for 10 min, and the temperature is then raised to 700℃. The mixture is calcined for 5 min, and then the furnace is cooled to room temperature. The calcined mixture is crushed and mixed uniformly with the pretreated filaments. The surface of the mixture is sprayed with a water extract of tobacco leaves, and the mixture is dried in the shade. Then, the surface of the mixture is sprayed with a water composite extract of Hippophae rhamnoides, and the mixture is dried in the shade.

[0035] The weight ratio of the leaf of Aquilaria sinensis, the water extract of tobacco leaves, and the water composite extract of Hippophae rhamnoides is 1:4:1.5.

[0036] The water extract of tobacco leaves is prepared by the following steps: 10 kg of tobacco leaves are soaked in 200 kg of water for 3 h, and then filtered with gauze and filter paper in sequence to obtain the water extract of tobacco leaves.

[0037] The sea-buckthorn water composite extract is prepared by the following steps: 10 kg of sea-buckthorn is soaked in 200 kg of water for 15 h, and then filtered with gauze and filter paper in sequence, 1 kg of honey and 0.1 kg of menthol are added into the filtrate and stirred uniformly to obtain the sea-buckthorn water composite extract.

[0038] Example 2: A preparation method of a composite moxa cigarette containing leaf of Aquilaria sinensis, comprising the following steps:

[0039] S1, 5 kg of wormwood straw is crushed and passed through a 100-mesh sieve, soaked in 60 kg of 0.5 mol / L sodium hydroxide solution, and placed at room temperature for 40 h, 2 kg of polyethylene glycol 500 is added, stirred at a speed of 150 r / min for 2 h, cooled to-35℃, and kept for 30 min, then warmed to 50℃ at a speed of 5℃ / min, and kept for 30 min, cooled to-40℃ again, and kept for 2 h, replaced with tert-butyl alcohol, 120 kg of leaf of Aquilaria sinensis is added and stirred uniformly, and then frozen and dried, cut into fine filaments with a width of 0.5±0.1 cm and a length of 0.7±0.1 cm to obtain pretreated filaments;

[0040] S2, 5 kg of cotton linters is vacuum dried at a temperature of 60℃ until constant weight, added into a tubular furnace, and warmed to 500℃ at a speed of 3℃ / min under nitrogen protection, calcined for 20 min, then warmed to 750℃, calcined for 15 min, and cooled to room temperature, crushed, mixed uniformly with the pretreated filaments, and then sprayed with water extract of tobacco leaves, and then mixed uniformly and air-dried, and then sprayed with sea-buckthorn water composite extract, and then mixed uniformly and air-dried.

[0041] The weight ratio of the leaf of Aquilaria sinensis, the water extract of tobacco leaves and the sea-buckthorn water composite extract is 1:3:1.2.

[0042] The water extract of tobacco leaves is prepared by the following steps: 30 kg of tobacco leaves is soaked in 350 kg of water for 5 h, and then filtered with gauze and filter paper in sequence to obtain the water extract of tobacco leaves.

[0043] The sea-buckthorn water composite extract is prepared by the following steps: 30 kg of sea-buckthorn is soaked in 350 kg of water for 30 h, and then filtered with gauze and filter paper in sequence, 3 kg of honey and 1 kg of menthol are added into the filtrate and stirred uniformly to obtain the sea-buckthorn water composite extract.

[0044] Example 3: A preparation method of a composite moxa cigarette containing leaf of Aquilaria sinensis, comprising the following steps:

[0045] S1, 2 kg of wormwood straw is crushed and passed through a 90 mesh sieve, soaked in 30 kg of 1.2 mol / L sodium hydroxide solution, room temperature for 25 h, add 1.7 kg of polyethylene glycol 1200, stirring at 80 r / min for 100 min, cooling to-25℃, incubate for 25 min, heating to 45℃ at a rate of 2℃ / min, incubate for 15 min, cooling to-37℃ again, incubate for 80 min, replace with tert-butyl alcohol, add 110 kg of Aquilaria sinensis leaves and stir evenly, freeze-drying, cut into 0.4±0.1 cm wide and 0.6±0.1 cm long filaments, to obtain pretreated filaments;

[0046] S2, 2 kg of cotton linters is dried to constant weight at 55℃ under vacuum, added to a tube furnace, heated to 480℃ at a rate of 1.5℃ / min under nitrogen protection, calcined for 12 min, continue to heat to 740℃, calcine for 8 min, cool to room temperature with the furnace, crush, mix evenly with the pretreated filaments, spray tobacco leaf water extract on the surface, mix evenly, then air dry, spray sea buckthorn water compound extract, mix evenly, then air dry.

[0047] The weight ratio of Aquilaria sinensis leaf, tobacco leaf water extract and sea buckthorn water compound extract is 1:5:2.

[0048] The tobacco leaf water extract is prepared by the following steps: 25 kg of tobacco leaves are soaked in 260 kg of water for 4.5 h, filtered with gauze and filter paper in turn to obtain the tobacco leaf water extract.

[0049] The sea buckthorn water compound extract is prepared by the following steps: 15 kg of sea buckthorn is soaked in 310 kg of water for 18 h, filtered with gauze and filter paper in turn, 2.5 kg of honey and 0.3 kg of menthol are added to the filtrate and stirred evenly to obtain the sea buckthorn water compound extract.

[0050] Example 4 A preparation method of a composite moxa cigarette containing Aquilaria sinensis leaves, comprising the following steps:

[0051] S1, 2 kg of wormwood straw is crushed and passed through a 90 mesh sieve, soaked in 30 kg of 1.2 mol / L sodium hydroxide solution, room temperature for 25 h, add 1.7 kg of polyethylene glycol 1200, stirring at 80 r / min for 100 min, cooling to-25℃, incubate for 25 min, heating to 45℃ at a rate of 2℃ / min, incubate for 15 min, cooling to-37℃ again, incubate for 80 min, replace with tert-butyl alcohol, add 110 kg of Aquilaria sinensis leaves and stir evenly, freeze-drying, cut into 0.4±0.1 cm wide and 0.6±0.1 cm long filaments, to obtain pretreated filaments;

[0052] S2, 4 kg of cotton linters were dried to constant weight at a temperature of 45℃ under vacuum, added to a tube furnace, and heated to 420℃ at a rate of 2.5℃ / min under nitrogen protection, calcined for 18 min, continued to heat to 720℃, calcined for 12 min, cooled to room temperature with the furnace, crushed, mixed uniformly with the pretreated filaments, sprayed with tobacco water extract on the surface, mixed and dried, then sprayed with sea buckthorn water compound extract, mixed and dried.

[0053] The weight ratio of white arborvitae leaf, tobacco water extract and sea buckthorn water compound extract is 1:2:1.

[0054] The tobacco water extract was prepared by the following steps: 15 kg of tobacco leaves were soaked in 300 kg of water for 3.5 h, and then filtered with gauze and filter paper in sequence to obtain the tobacco water extract.

[0055] The sea buckthorn water compound extract was prepared by the following steps: 25 kg of sea buckthorn was soaked in 270 kg of water for 24 h, and then filtered with gauze and filter paper in sequence, 1.5 kg of honey and 0.7 kg of menthol were added to the filtrate and stirred uniformly to obtain the sea buckthorn water compound extract.

[0056] Example 5 A preparation method of a white arborvitae leaf composite fumigation and moxibustion cigarette, comprising the following steps:

[0057] S1, 3 kg of wormwood straw was crushed and passed through an 80 mesh sieve, soaked in 40 kg of 1 mol / L sodium hydroxide solution, and placed at room temperature for 30 h, 1.5 kg of polyethylene glycol 1200 was added, stirred at a speed of 100 r / min for 90 min, cooled to -28℃, heated to 40℃ at a rate of 3℃ / min, and kept for 20 min, cooled to -35℃ again, and kept for 90 min, replaced with tert-butyl alcohol, 100 kg of white arborvitae leaves were added and stirred uniformly, freeze-dried, cut into filaments with a width of 0.4±0.1 cm and a length of 0.6±0.1 cm to obtain pretreated filaments;

[0058] S2, 3 kg of cotton linters were dried to constant weight at a temperature of 50℃ under vacuum, added to a tube furnace, and heated to 450℃ at a rate of 2℃ / min under nitrogen protection, calcined for 15 min, continued to heat to 730℃, calcined for 10 min, cooled to room temperature with the furnace, crushed, mixed uniformly with the pretreated filaments, sprayed with tobacco water extract on the surface, mixed and dried, then sprayed with sea buckthorn water compound extract, mixed and dried.

[0059] The weight ratio of white arborvitae leaf, tobacco water extract and sea buckthorn water compound extract is 1:2:2.

[0060] The tobacco leaf water extract is prepared by the following steps: 20 kg of tobacco leaves are soaked in 280 kg of water for 4 hours, and then filtered with gauze and filter paper in sequence to obtain the tobacco leaf water extract.

[0061] The sea buckthorn water composite extract is prepared by the following steps: 20 kg of sea buckthorn is soaked in 300 kg of water for 21 hours, and then filtered with gauze and filter paper in sequence, 2 kg of honey and 0.5 kg of menthol are added into the filtrate and stirred uniformly to obtain the sea buckthorn water composite extract.

[0062] Comparative Example 1

[0063] A preparation method of a composite moxa cigarette containing leaf of Aquilaria sinensis, comprising the following steps:

[0064] S1, 100 kg of leaf of Aquilaria sinensis is cut into fine filaments with a width of 0.4±0.1 cm and a length of 0.6±0.1 cm to obtain pretreated filaments;

[0065] S2, 3 kg of cotton linters is dried under vacuum at 50℃ to a constant weight, added into a tubular furnace, and heated to 450℃ at a speed of 2℃ / min under nitrogen protection, calcined for 15 min, continuously heated to 730℃, calcined for 10 min, cooled to room temperature with the furnace, and pulverized, mixed uniformly with the pretreated filaments, sprayed with tobacco leaf water extract on the surface, mixed uniformly, air-dried, sprayed with sea buckthorn water composite extract, mixed uniformly, and air-dried.

[0066] The weight ratio of the leaf of Aquilaria sinensis, the tobacco leaf water extract and the sea buckthorn water composite extract is 1:2:2.

[0067] The tobacco leaf water extract is prepared by the following steps: 20 kg of tobacco leaves are soaked in 280 kg of water for 4 hours, and then filtered with gauze and filter paper in sequence to obtain the tobacco leaf water extract.

[0068] The sea buckthorn water composite extract is prepared by the following steps: 20 kg of sea buckthorn is soaked in 300 kg of water for 21 hours, and then filtered with gauze and filter paper in sequence, 2 kg of honey and 0.5 kg of menthol are added into the filtrate and stirred uniformly to obtain the sea buckthorn water composite extract.

[0069] Comparative Example 2

[0070] A preparation method of a composite moxa cigarette containing leaf of Aquilaria sinensis, comprising the following steps:

[0071] S1, 3 kg of wormwood straw was crushed and passed through an 80 mesh sieve, soaked in 40 kg of 1 mol / L sodium hydroxide solution, room temperature for 30 h, 1.5 kg of polyethylene glycol 1200 was added, stirred at 100 r / min for 90 min, cooled to-28℃, incubated for 20 min, heated to 40℃ at a rate of 3℃ / min, incubated for 20 min, cooled to-35℃ again, incubated for 90 min, replaced with tert-butyl alcohol, 100 kg of Aquilaria sinensis leaves were added and stirred evenly, freeze-dried, cut into fine filaments with a width of 0.4±0.1 cm and a length of 0.6±0.1 cm, and pretreated filaments were obtained;

[0072] S2, the surface of the pretreated filaments was sprayed with tobacco water extract, mixed and dried, then sprayed with sea buckthorn water extract, mixed and dried.

[0073] The weight ratio of Aquilaria sinensis leaves, tobacco water extract and sea buckthorn water extract is 1:2:2.

[0074] The tobacco water extract was prepared by the following steps: 20 kg of tobacco was soaked in 280 kg of water for 4 h, and then filtered with gauze and filter paper in sequence to obtain the tobacco water extract.

[0075] The sea buckthorn water extract was prepared by the following steps: 20 kg of sea buckthorn was soaked in 300 kg of water for 21 h, and then filtered with gauze and filter paper in sequence, 2 kg of honey and 0.5 kg of menthol were added to the filtrate and stirred evenly to obtain the sea buckthorn water extract.

[0076] The fumigation incense obtained in Example 5 and Comparative Examples 1-2 was ignited, and the highest temperature and the lowest temperature in the burning process were measured at a distance of 1 cm from the burning end of each group of incense. The concentration of smoke generated by the fumigation incense obtained in Example 5 and Comparative Examples 1-2 was tested by a portable microcomputer dust instrument P-SL2C, and the concentration of PM10 in the smoke was detected by light scattering method.

[0077] As shown in Figure 1 , the temperature fluctuation of the fumigation incense obtained in Example 5 was small, always maintained at 47-58℃, and the human body test evaluated its moxibustion feeling as surface not hot, deep hot, local not hot and surrounding hot, which was very suitable for fumigation and moxibustion; at the same time, the PM10 concentration of the smoke generated by burning was the lowest, which greatly reduced the harmful substances generated by insufficient burning and greatly reduced the adverse effects on the human body.

[0078] The applicant believes that: this is due to the fact that the application first alkali treatment of artemisia straw and polyethylene glycol freeze-thaw-regeneration, the generated fiber structure is extremely loose, load on the surface of the leaf of lindera glauca, good affinity, high mutual combination strength, the loose fiber structure of lindera glauca can promote the full combustion, greatly reduce the content of harmful substances produced by incomplete combustion, greatly reduce the adverse effects on the human body; then the cotton linters are used as raw materials to form a porous hydrophobic structure with high specific surface area after carbonization, the highly porous, high specific surface area and hydrophobic structure can quickly adsorb the tar produced by combustion, with high adsorption capacity and less tar pollution, further reducing the harm of flue gas, at the same time, its ignition point is much higher than the combustion temperature of lindera glauca, so the shell will not be ignited during use, and its strong adsorption not only can adsorb heat to reduce the body temperature of human body, but also can adsorb toxic and harmful gases produced by combustion to avoid harm to the user; when the temperature is low at the tail end of the combustion, the absorbed heat can be released, thereby moderating the temperature change.

[0079] Take 72 SPF Wistar rats (weight 180±20g), half male and half female; the standard accommodation conditions of the animals are temperature 22±2℃, humidity 55-60%, light and dark alternation each 12h, free diet and water. The rats are adaptively fed for 3d, weighed and labeled, and then randomly grouped (normal group, model group, positive drug group, example 5 group, comparative example 1 group, comparative example 2 group), 3 repeats in each group, 4 rats in each repeat. Except that the rats in the normal group are all modeled: the left hind foot sole skin is routinely disinfected, and then 1mL syringe is used for intradermal injection of complete Freund's adjuvant (0.1mL / one).

[0080] After modeling for 3d, the success of modeling is evaluated according to the arthritis index (arthritis index, AI) score. The success of modeling is determined by the following criteria: no joint redness is 0 points, foot toe joint redness is 1 point, toe joint and toe swelling is 2 points, ankle joint redness is 3 points, and all paw swelling including ankle joint is 4 points. The AI value is the sum of the scores of each rat. The score of 6 points or more is the success of modeling.

[0081] The positive drug group adopts methotrexate sodium chloride solution (mixing methotrexate uniformly in 0.9% sodium chloride solution), and is filled into the stomach at a dose of 0.1 mg / 100 g, twice a week (with an interval of 3-4 days each time), and is filled into the stomach for 3 weeks. The example 5 group, the comparative example 1 group and the comparative example 2 group respectively adopt the fumigating moxibustion cigarettes obtained in example 5 and comparative examples 1-2 for suspension moxibustion treatment: fixing the moxa sticks on the modified suspension moxibustion support, and performing moxibustion at a position 2 cm away from the kidney acupoint (kidney acupoint is located below the second lumbar spine, and is about 7 mm apart on the left and right sides) and the back three li acupoint (back three li is the posterior lateral of the knee joint, 5 mm below the small head of the fibula), and moxibustion is performed for 20 min for each acupoint, and the two acupoints are treated at the same time, and the left and right acupoints are alternated, once a day, and 6 days of continuous moxibustion treatment is a course of treatment, and three courses of treatment are performed, and 1 day is rested between the courses of treatment.

[0082] Arthritis inflammation AI scores were performed after modeling and on the last day of each cycle, and the results are shown in Figure 2 After the intervention, the rats were fasted and deprived of water for 12 h, anesthetized by intraperitoneal injection of 1% sodium pentobarbital (50 mg / kg), fixed on the operating table, and blood samples were taken from the abdominal aorta. The whole blood samples were placed at room temperature for 1 h and then centrifuged for 15 min (3000 r / min). The supernatant was stored at -20°C, and then the levels of TNF-α, IL-1β, IFN-γ and IL-4 in the serum of the rats were detected by ELISA, and the results are shown in Figure 3 and Figure 4

[0083] As can be seen from Figures 2-4 : the suspension moxibustion treatment with the fumigating moxibustion cigarettes can effectively improve joint redness, reduce inflammatory cytokines TNF-α and IL-1β, and improve IFN-γ / IL-4. At the same time, the suspension moxibustion treatment with the fumigating moxibustion cigarettes obtained in example 5 is better than methotrexate, which confirms the effect on rheumatoid arthritis and can overcome the adverse reactions such as gastrointestinal reactions caused by oral drugs.

[0084] The above describes only the preferred specific embodiments of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can make equivalent replacements or changes to the technical solutions and the inventive concept of the present application within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.​

Claims

1. A method for preparing a compound fumigation cigarette containing Aquilaria sinensis leaves, characterized in that, Includes the following steps: S1. Crush and sieve the Artemisia argyi stalks, soak them in sodium hydroxide solution, let them stand at room temperature for 20-40 hours, add polyethylene glycol and stir for 1-2 hours, cool down to -20 to -35℃, keep warm for 10-30 minutes, heat up to 30-50℃ and keep warm for 10-30 minutes, cool down again to -30 to -40℃ and keep warm for 1-2 hours, replace with tert-butanol, add Aucklandia lappa leaves and stir evenly, freeze dry, and cut into shreds to obtain pre-treated fine shreds; S2. Vacuum dry the cotton linters to constant weight, add them to a tube furnace, heat to 400-500℃ under nitrogen protection, calcine for 10-20 minutes, continue heating to 700-750℃, calcine for 5-15 minutes, cool to room temperature with the furnace, pulverize, mix evenly with pretreated fine fibers, spray tobacco leaf aqueous extract on its surface, mix well and air dry, then spray sea buckthorn aqueous compound extract, mix well and air dry.

2. The method for preparing the compound fumigation cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, In S1, the mass ratio of Artemisia argyi stalks, polyethylene glycol, and Aquilaria sinensis leaves is 1-5:1-2:80-120.

3. The method for preparing the compound fumigation cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, In S1, the concentration of sodium hydroxide solution is 0.5-1.5 mol / L.

4. The method for preparing the compound fumigation cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, In S1, the molecular weight of polyethylene glycol is 500-1500.

5. The method for preparing the compound fumigation cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, In S1, the heating rate is 1-5℃ / min during the process of heating to 30-50℃.

6. The method for preparing the compound moxibustion cigarette containing Aquilaria sinensis leaves according to claim 1, characterized in that, The mass ratio of cotton linters used in S2 to mugwort straw used in S1 is 1-5:1-5.

7. The method for preparing the compound fumigation cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, In S2, the vacuum drying temperature for cotton linters is 40-60℃.

8. The method for preparing the compound fumigation cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, In S2, the heating rate is 1-3℃ / min during the process of heating to 400-500℃.

9. The method for preparing the compound fumigation cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, In S2, the tobacco leaf aqueous extract is prepared by the following steps: soaking tobacco leaves in water for 3-5 hours and filtering to obtain the tobacco leaf aqueous extract; the mass ratio of tobacco leaves to water is 10-30:200-350.

10. The method for preparing the compound moxibustion cigarette containing Aucklandia lappa leaves according to claim 1, characterized in that, The sea buckthorn aqueous compound extract is prepared by the following steps: soaking sea buckthorn in water for 15-30 hours, filtering, adding honey and menthol to the filtrate and stirring evenly to obtain the sea buckthorn aqueous compound extract; the mass ratio of sea buckthorn, water, honey and menthol is 10-30:200-350:1-3:0.1-1.

11. A compound fumigation cigarette containing Aucklandia lappa leaves, characterized in that, The smoke is prepared using the method described in any one of claims 1-10, which involves the preparation of a compound moxibustion cigarette containing Aquilaria sinensis leaves.

Images