Burrowing bivalve physiological experiment device and culture method and experiment method

Abstract

This invention discloses a physiological experimental device and cultivation method for burrowing shellfish, comprising: a box with an internal accommodating space; an inlet pipe located at the upper part of the box, on which a first flow control element is installed; a first outlet pipe located at the upper part of the box, its height lower than the inlet pipe; a second outlet pipe located at the bottom of the box, on which a second flow control element is installed; two buffer partitions located near the inlet pipe and the first outlet pipe, respectively, to divide the box into a buffer chamber and a cultivation chamber; a cultivation net cage placed inside the cultivation chamber; and a controller communicating with the first and second flow control elements. This novel physiological experimental device for burrowing shellfish can accurately simulate the actual marine environment in which burrowing shellfish grow, making the cultured shellfish closely resemble those cultured in the sea, thus ensuring the accuracy of the obtained parameters.

Description

Technical Field

[0001] This invention belongs to the field of burrowing shellfish aquaculture technology. Specifically, it relates to a physiological experimental device for burrowing shellfish and its corresponding aquaculture and experimental methods. Background Technology

[0002] Burrowing shellfish are an important biological species for expanding the utilization of the bottom space of shallow sea mudflats in my country.

[0003] The supply of appropriately sized seedlings is a crucial aspect of aquaculture. Currently, provinces such as Shandong, Liaoning, Fujian, and Zhejiang have successfully carried out seedling breeding and aquaculture of various burrowing shellfish, including Manila clams, hard clams, and blood clams. Bottom-seeding shellfish seedlings is a major task in tidal flat shellfish aquaculture and a key pathway to increasing production and income in tidal flat shellfish farming.

[0004] To study and obtain various parameters of burrowing shellfish, it is necessary to place the shellfish in artificial culture containers for cultivation, and then obtain some parameters that need to be studied, such as growth index parameters, or to measure the respiration rate and calcification rate of the shellfish in order to establish a carbon sink assessment model for marine burrowing shellfish culture.

[0005] Current experimental culture of burrowing shellfish mainly involves placing the shellfish in a plastic or glass container, adding seawater directly inside, and then collecting the required parameters after a period of culture. This culture method differs greatly from the actual living environment of the shellfish, resulting in poor accuracy of the obtained experimental parameters and significant bias in the research results.

[0006] The information disclosed in this background section is only intended to enhance the understanding of the background technology of this application, and therefore may include prior art that is not known to those skilled in the art. Summary of the Invention

[0007] This invention addresses the aforementioned technical problems in the experimental culture of burrowing shellfish in existing technologies by proposing a novel physiological experimental device for burrowing shellfish. This device can accurately simulate the actual marine environment in which burrowing shellfish grow, making the cultured shellfish similar to those cultured in the sea, thereby ensuring the accuracy of the obtained parameters.

[0008] To achieve the above-mentioned invention / design objectives, the present invention adopts the following technical solution:

[0009] A physiological experimental device for burrowing mollusks, comprising a box with an internal space for containment;

[0010] A water inlet pipe is located at the upper part of the tank, and a first flow control element is installed on the water inlet pipe;

[0011] The first water outlet pipe is located at the upper part of the tank, and its height is lower than that of the water inlet pipe;

[0012] The second water outlet pipe is located at the bottom of the tank, and a second flow control element is installed on the second water outlet pipe.

[0013] Two buffer partitions are provided, which are respectively arranged near the water inlet pipe and the first water outlet pipe to divide the tank into a buffer chamber and a breeding chamber in sequence. The bottom of the breeding chamber is covered with sediment.

[0014] A breeding net cage is placed inside the breeding chamber, and multiple breeding mesh holes are formed on the wall of the breeding net cage;

[0015] The controller communicates with the first flow control element and the second flow control element, and is able to control the opening degree of the first flow control element and the second flow control element.

[0016] In some embodiments of this application, the aquaculture cage includes:

[0017] The cage itself has an internal aquaculture space;

[0018] And the cage handle connected to the cage body;

[0019] Multiple conical protrusions are formed on the bottom of the cage body, and the multiple conical protrusions are evenly arranged at the intersection of adjacent aquaculture mesh openings.

[0020] In some embodiments of this application, the buffer baffle includes:

[0021] The partition body has multiple partition holes that are evenly distributed throughout it.

[0022] A flexible buffer element, detachably connected at the top of the baffle body, has a guide surface that directs the water flow.

[0023] In some embodiments of this application, a partition component is also included, arranged inside the breeding chamber to divide the breeding chamber into multiple sub-breeding chambers, and the breeding net cage is provided in each of the sub-breeding chambers;

[0024] The separating component includes:

[0025] The first partition is positioned between the two buffer partitions;

[0026] The second partition plate is set perpendicular to the first partition plate and is connected and fixed to the first partition plate.

[0027] In some embodiments of this application, a partition plate assembly is also included, the partition plate assembly including a first partition plate, a second partition plate and a third partition plate;

[0028] And the cover module;

[0029] A first slot with a top opening is provided on the body of the partition for inserting the first partition plate;

[0030] The first partition plate is provided with a second slot with a top opening for inserting the second partition plate;

[0031] The second partition plate is provided with a third slot with a top opening for inserting the third partition plate;

[0032] The experimental apparatus for the physiological functions of burrowing mollusks includes a first experimental mode and a second experimental mode.

[0033] In the first experimental mode, the first partition plate, the second partition plate and the third partition plate are respectively inserted into the first slot, the second slot and the third slot, and the cover module is placed on top of the sub-culture chamber to seal the multiple sub-culture chambers.

[0034] In the second experimental mode, the first partition, the second partition and the third partition are pulled out from the first slot, the second slot and the third slot respectively, the cover module is away from the sub-breeding chamber, and the multiple sub-breeding chambers are interconnected and connected to the buffer chamber.

[0035] In some embodiments of this application, the height of the first water outlet pipe is higher than that of the buffer barrier.

[0036] In some embodiments of this application, a control culture container is also provided on the side of the box, and a control culture space is formed inside the control culture container.

[0037] A method for cultivating burrowing shellfish based on a physiological experimental device for burrowing shellfish includes the following steps:

[0038] Place the aquaculture cages into the aquaculture chamber;

[0039] Lay sediment inside the aquaculture cages, making it lower than or level with the top surface of the aquaculture cages;

[0040] Open the inlet pipe, close the second outlet pipe, and open the first outlet pipe;

[0041] Water is injected into the cage at a certain flow rate. When the water level reaches the first preset height, burrowing shellfish are released into the aquaculture cage.

[0042] The first preset time for raising burrowing shellfish in the container;

[0043] The controller controls the inlet water flow rate to alternate between high and low within a certain flow range until the water level in the tank is level with the first outlet water pipe.

[0044] When the water level in the tank reaches the same level as the first water outlet pipe, the first water outlet pipe is kept open for a second preset time.

[0045] The inlet pipe is closed and the outlet pipe is opened. The flow rate of the outlet pipe is controlled to alternate between high and low within a certain flow range until all the water in the tank is discharged.

[0046] The control system keeps the inlet pipe closed, the first outlet pipe and the second outlet pipe open for a third preset time.

[0047] An experimental method for studying the performance parameters of burrowing mollusks based on the aforementioned physiological experimental apparatus for burrowing mollusks includes the following steps: placing the burrowing mollusks:

[0048] Multiple aquaculture cages were placed into multiple burial shellfish physiological experimental devices;

[0049] Sediment was laid inside multiple aquaculture cages;

[0050] Open the inlet pipe and the first outlet pipe of each burrowing shellfish physiological experimental device, close the second inlet pipe, and control the inlet pipe to inject water into the tank at the maximum flow rate so that the water level is at least higher than the height of the sediment.

[0051] Place the same number and size of burrowing shellfish in multiple aquaculture cages;

[0052] Water injection aquaculture: The water level in the tank is injected to the level of the first outlet pipe by controlling the water inlet pipe of multiple burial shellfish physiological experimental devices in a variable flow manner. The time required for each burial shellfish physiological experimental device is the water injection time, and the water injection time of multiple burial shellfish physiological experimental devices increases in an incremental manner.

[0053] After the internal position of the tank reaches the level of the first water outlet pipe, keep multiple water inlet pipes open for the first holding time.

[0054] Drainage culture: The second outlet pipe of multiple burial shellfish physiological experimental devices is controlled to drain the water in the tank in a variable flow manner. The time required for each burial shellfish physiological experimental device is the drainage time, and the drainage time of multiple burial shellfish physiological experimental devices varies in descending order.

[0055] After the water is drained from the tank, keep multiple inlet pipes closed and the second outlet pipe open for a second holding time.

[0056] The alternating cycle of water-filled and water-drained aquaculture was controlled, and multiple aquaculture cages were removed after a preset aquaculture period to extract growth parameters of the burrowing shellfish in the cages.

[0057] A method for determining the respiratory metabolic rate of burrowing mollusks based on a physiological experimental apparatus for burrowing mollusks:

[0058] After a pre-set breeding period, the first partition plate, the second partition plate, and the third partition plate in the physiological experimental device for burial shellfish with burial shellfish are respectively inserted into the first slot, the second slot, and the third slot. The cover module is placed on top of the sub-breeding chamber to seal the multiple sub-breeding chambers.

[0059] At the same time, a control burrowing shellfish physiological experiment device was set up and sealed, and no burrowing shellfish were placed inside it;

[0060] After a preset time period, the dissolved oxygen content and ammonia nitrogen concentration in the seawater of the burial shellfish physiological experimental device were measured. The respiratory metabolic rate of the burial shellfish was obtained based on the dissolved oxygen content and ammonia nitrogen concentration in the control burial shellfish physiological experimental device and the dissolved oxygen content and ammonia nitrogen concentration in the burial shellfish physiological experimental device.

[0061] Compared with the prior art, the advantages and positive effects of the present invention are:

[0062] The physiological experimental device for burrowing shellfish in this invention, by placing sediment in the aquaculture cage and installing an inlet pipe, a first outlet pipe, and a second outlet pipe on the cage, creates a growth environment for the burrowing shellfish located in the aquaculture chamber that closely resembles the tidal growth environment in the sea. This ensures that the parameters of the cultured burrowing shellfish are similar to those of the sea, thereby guaranteeing the accuracy of the shellfish measurement parameters and the precision of the experiment, providing accurate data references for scientific research.

[0063] Other features and advantages of the present invention will become clearer after reading the detailed embodiments of the invention in conjunction with the accompanying drawings. Attached Figure Description

[0064] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0065] Figure 1 This is a three-dimensional structural schematic diagram of an embodiment of the physiological experimental device for burrowing mollusks proposed in this invention;

[0066] Figure 2 This is a three-dimensional structural schematic diagram of another embodiment of the physiological experimental device for burrowing mollusks proposed in this invention;

[0067] Figure 3 This is a schematic diagram of the structure of the aquaculture net cage of the burial shellfish physiological experimental device proposed in this invention, placed inside the cage.

[0068] Figure 4 This is a schematic diagram of the structural arrangement of the buffer partition and the separation component in one embodiment of the physiological experimental device for burrowing mollusks proposed in this invention.

[0069] Figure 5 This is a schematic diagram of the structure of the aquaculture net cage, buffer partition, and separation components in one embodiment of the physiological experimental device for burial shellfish proposed in this invention.

[0070] Figure 6 This is a schematic diagram of the structure of a buffer partition in one embodiment of the physiological experimental device for burrowing mollusks proposed in this invention;

[0071] Figure 7 This is a three-dimensional structural diagram of a culture net cage according to an embodiment of the physiological experimental device for burrowing shellfish proposed in this invention;

[0072] Figure 8 This is a top view of a culture net cage according to an embodiment of the physiological experimental device for burrowing shellfish proposed in this invention;

[0073] Figure 9 for Figure 8 Sectional view along axis AA;

[0074] Figure 10 This is a schematic diagram of the respiratory metabolic rate of a physiological experimental device for burrowing mollusks proposed in one embodiment of the present invention. Figure 1 ;

[0075] Figure 11 This is a schematic diagram of the respiratory metabolic rate of a physiological experimental device for burrowing mollusks proposed in one embodiment of the present invention. Figure 2 ;

[0076] Figure 12 This is a schematic diagram of the respiratory metabolic rate of a physiological experimental device for burrowing mollusks proposed in one embodiment of the present invention. Figure 3 .

[0077] In the diagram, 100 is the enclosure; 110 is the buffer chamber; 120 is the rearing chamber; 121 is the sub-rearing chamber; 130 is the control rearing space; 200 is the water inlet pipe; 210 is the first flow control element; 300 is the first water outlet pipe; 400 is the second water outlet pipe; 410 is the second flow control element; 500 is the buffer baffle; 510 is the baffle body; 511 is the baffle hole; 512 is the first slot; 520 is the flexible buffer; 521 is the guide surface; 600 is the rearing cage; 610 is the rearing mesh. 630, Net cage handle; 640, Conical protrusion; 710, First partition plate; 711, Second slot; 720, Second partition plate; 721, Third slot; 800, Control culture container; 910, First baffle plate; 920, Second baffle plate; 930, Third baffle plate; 940, Cover module; 941, Sub-cover; 950, Detection probe. Detailed Implementation

[0078] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0079] In the description of this invention, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0080] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances. In the description of embodiments, specific features, structures, materials, or characteristics can be combined in any suitable manner in one or more embodiments or examples.

[0081] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature.

[0082] In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0083] This invention proposes an embodiment of a physiological experimental device for burrowing mollusks, comprising:

[0084] The box is 100mm in size, and its interior has a storage space.

[0085] A water inlet pipe 200 is arranged at the upper part of the housing 100, and a first flow control element 210 is provided on the water inlet pipe 200.

[0086] In some embodiments of this application, the water inlet pipe 200 is located near the top surface of the tank 100 and is connected to one side wall of the tank 100. It communicates with the accommodating space inside the tank 100 and can be used to deliver water into the tank 100.

[0087] The first flow control element 210 is a first flow control valve, which is connected to the water inlet pipe 200 and can control the water inlet flow of the water inlet pipe 200.

[0088] Of course, during use, the opening of the first flow control valve can also be controlled to 0, so that it closes the entire inlet pipe 200.

[0089] The first water outlet pipe 300 is located at the upper part of the box 100, and its height is lower than that of the water inlet pipe 200.

[0090] When the water level inside the tank 100 reaches the height of the first outlet pipe 300, the water level inside the tank 100 is kept stable by the combination of water inlet pipe 200 and water outlet pipe 300. At the same time, the water flow inside the tank 100 is ensured to be a continuous flow from inlet to outlet, making it closer to the marine living environment of shellfish.

[0091] The second water outlet pipe 400 is arranged at the bottom of the box 100, and a second flow control element 410 is provided on the second water outlet pipe 400.

[0092] In some embodiments of this application, the second water outlet pipe 400 is arranged on the other side wall of the tank 100 and close to the bottom surface of the tank 100. It is arranged opposite to the water inlet pipe 200 and is mainly used to discharge the water inside the tank 100 to the outside.

[0093] In some embodiments of this application, the second flow control element 410 is a second flow control valve, which can adjust the flow rate of the water outlet pipe 400 by adjusting the opening degree.

[0094] Two buffer partitions 500 are provided and are respectively arranged near the water inlet pipe 200 and the first water outlet pipe 300 to divide the box 100 into a buffer chamber 110 and a breeding chamber 120 in sequence.

[0095] A culture cage 600 is placed inside the culture chamber 120. Multiple culture mesh holes 610 are formed on the wall of the culture cage 600. The multiple culture mesh holes 610 can ensure the air permeability of the entire culture cage 600, ensuring that the buried shellfish can carry out normal respiration and growth inside. On the other hand, the culture mesh holes 610 can ensure that water can enter the interior of the culture cage 600 for shellfish culture when it is placed inside the culture chamber 120.

[0096] Sediment was laid inside the aquaculture cage 600;

[0097] In some embodiments of this application, the buffer barrier 500 includes:

[0098] The partition body 510 has partition holes 511 provided on it. Multiple partition holes 511 are provided and evenly distributed throughout the partition body 510.

[0099] The flexible buffer 520 is detachably connected to the top of the baffle body 510 and has a guide surface 521 for guiding water flow.

[0100] The baffle body 510 is a buffer baffle plate, which is vertically arranged inside the box 100. The top of the buffer baffle plate is a certain distance away from the top surface of the box 100 so that a flow space is formed between the buffer baffle 500 and the top of the box 100.

[0101] The buffer chamber 110 is formed by the buffer baffle 500 and the wall of the tank 100. In the water flow path, the buffer chamber 110 is located upstream of the aquaculture chamber 120.

[0102] The breeding chamber 120 is formed by two opposing buffer partitions 500 and the wall of the box body 100.

[0103] During aquaculture, when the aquaculture cage 600 is placed inside the aquaculture chamber 120, sediment is laid inside. Two buffer baffles 500 can also be used to separate the sediment in the aquaculture cage 600, preventing it from being continuously lost with the water flow and ensuring the aquaculture effect.

[0104] In some embodiments of this application, the sediment is silt or sand, so as to make it more similar to the environment in which shellfish live in marine silt.

[0105] In some embodiments of this application, the water flowing in from the inlet pipe 200 first enters the buffer chamber 110, and then flows into the breeding chamber 120 through the partition hole 511 on the buffer partition 500.

[0106] The baffle hole 511 on the buffer baffle 500 can buffer the water flow to a certain extent, so that the water flow can flow smoothly and evenly into the breeding chamber 120.

[0107] In some embodiments of this application, the pore size of the buffer hole is 0.2-2 mm to increase the water permeability and air permeability of the sediment.

[0108] The flexible buffer 520 is a flexible buffer sheet, a silk screen, or a filter cotton sheet, which buffers and guides the water flow so that when the water level in the buffer chamber 110 is higher than that in the baffle body 510, the water flowing into the aquaculture chamber 120 is less likely to stir up sediment.

[0109] It is detachably connected to the partition body 510 and can be removed or installed on the partition body 510 as needed.

[0110] When the water inlet pipe 200 is opened, the water flow first enters the buffer chamber 110. When the water flow height in the buffer chamber 110 exceeds the buffer baffle 500, it will enter the breeding chamber 120 through the upper flow space.

[0111] The controller communicates with the first flow control element 210 and the second flow control element 410 and is able to control the opening degree of the first flow control element 210 and the second flow control element 410.

[0112] When cultivating burrowing shellfish, the controller can control the switching of the first flow control element 210 and the flow rate, so that water can enter the inlet pipe 200 to simulate the tidal environment during the growth of burrowing shellfish.

[0113] By controlling the opening and closing of the second outlet pipe 400 and the outlet flow rate through the second flow control element 410, the tank 100 is drained, thereby simulating the low tide environment during the growth of burial shellfish, so that the growth environment of the burial shellfish growing inside is almost close to the marine environment.

[0114] When setting up the cages, a certain amount of seaweed can be placed inside to make the growth environment of the buried shellfish closer to the marine environment.

[0115] The burrowing shellfish physiological experimental device in this embodiment, by placing sediment in the aquaculture cage 600 and installing an inlet pipe 200, a first outlet pipe 300, and a second outlet pipe 400 on the cage body 100, works together to make the growth environment of the burrowing shellfish located in the aquaculture chamber 120 similar to the tidal growth environment in the sea. Therefore, the parameters of the cultured burrowing shellfish are similar to those of the sea, which can ensure the accuracy of the shellfish measurement parameters, ensure the accuracy of the experiment, and provide accurate data reference for scientific research.

[0116] In some embodiments of this application, the aquaculture cage 600 includes:

[0117] The cage itself has an internal aquaculture space;

[0118] and a cage handle 630 connected to the cage body;

[0119] The net cage handle 630 can be a U-shaped net cage handle 630, or handles can be provided on both sides of the net cage body. The net cage handle 630 can be used to easily place the aquaculture net cage 600 into the aquaculture chamber 120 or take it out of the aquaculture chamber 120.

[0120] Multiple conical protrusions 640 are formed on the bottom of the cage body, and the multiple conical protrusions 640 are evenly arranged at the intersection of adjacent aquaculture mesh 610.

[0121] In some embodiments of this application, the aperture of the aquaculture mesh 610 is 5-8mm, which ensures both the air permeability of the aquaculture cage 600 and prevents the buried shellfish from falling out of the aquaculture cage 600.

[0122] The conical protrusion 640 at the bottom of the cage body can loosen the mud and sand when lifted, making it easier to collect experimental shellfish.

[0123] In some embodiments, the top of the aquaculture cage 600 may be configured as an openable or closable cover structure to facilitate the release and retrieval of buried shellfish.

[0124] In some embodiments of this application, a partition component is also included, arranged within the breeding chamber 120 to divide the breeding chamber 120 into multiple sub-breeding chambers 121, which are interconnected, and the breeding net cage 600 is provided in each of the sub-breeding chambers 121.

[0125] The multiple sub-culture chambers 121 formed by the separation components allow the entire experimental device to simultaneously culture multiple groups of burrowing shellfish, thus forming multiple parallel groups.

[0126] When measuring parameters of burrowing shellfish, the accuracy of the measurement parameters can be further ensured by averaging the data from multiple sets of burrowing shellfish.

[0127] In some embodiments of this application, the separating component includes:

[0128] The first partition plate 710 is arranged between the two buffer partitions 500;

[0129] The second partition plate 720 is set perpendicular to the first partition plate 710 and is connected and fixed to the first partition plate 710.

[0130] The first partition plate 710 is provided with a plurality of first partition holes, and the second partition plate 720 is provided with a plurality of second partition holes.

[0131] The first and second partition holes on the two partition plates can be used to connect the various sub-breeding chambers 121 to each other, ensuring that the water flowing into the tank 100 eventually flows into the corresponding sub-breeding chamber 121 through the first and second partition holes.

[0132] The breeding chamber 120 is divided into four sub-breeding chambers 121 by the first partition plate 710 and the second partition plate 720, two of which are close to the buffer chamber 110.

[0133] The water flow first enters the two sub-breeding chambers 121 near the buffer chamber 110, and then enters the other two breeding chambers 120 connected to the two sub-breeding chambers 121.

[0134] A baffle cavity is also formed between the buffer baffle 500 near the first water outlet pipe 300 and the wall of the tank 100. The sediment flowing out of the breeding chamber 120 can settle in the baffle cavity to a certain extent, thereby ensuring that it will not block the second water outlet pipe 400.

[0135] In some embodiments of this application, the first water outlet pipe 300 is higher than the buffer baffle 500 and lower than the water inlet pipe 200. When the water level exceeds the buffer baffle 500, the water level is kept stable during the experiment.

[0136] In some embodiments of this application, a partition plate assembly is also included, the partition plate assembly including a first partition plate 910, a second partition plate 920 and a third partition plate 930;

[0137] And cover module 940;

[0138] A first slot 512 with a top opening is provided on the partition body 510 for inserting the first partition plate 910;

[0139] The first partition plate 710 is provided with a second slot 711 with a top opening for inserting the second partition plate 920;

[0140] The second partition plate 720 is provided with a third slot 721 with a top opening for inserting the third partition plate 930;

[0141] The experimental apparatus for the physiological functions of burrowing mollusks includes a first experimental mode and a second experimental mode.

[0142] In the first experimental mode, the flexible buffer is removed, and then the first partition plate 910, the second partition plate 920 and the third partition plate 930 are respectively inserted into the first slot 512, the second slot 711 and the third slot 721. The cover module 940 is placed on top of the sub-breeding chamber 121 to seal the multiple sub-breeding chambers 121.

[0143] In the second experimental mode, the first partition plate 910, the second partition plate 920 and the third partition plate 930 are pulled out from the first slot 512, the second slot 711 and the third slot 721 respectively, and the cover module 940 is moved away from the sub-breeding chamber 121. The multiple sub-breeding chambers 121 are interconnected and connected to the buffer chamber.

[0144] After the first baffle plate 910 is inserted into the first slot 512 inside the baffle body 510, it can separate the baffle hole 511 on the baffle body 510, so that the water flow will no longer enter the sub-breeding chamber 121 through the buffer chamber.

[0145] When sealed, the buffer partitions 500 on both sides of the box 100 are separated by the first partition plate 910.

[0146] The second partition plate 920 can be inserted into the first partition plate 710 to separate the first partition hole, so that it is no longer connected to each sub-breeding chamber 121.

[0147] The third partition plate 930 is inserted into the second partition plate 720 to separate the second partition hole on the second partition plate 720.

[0148] In some embodiments, when the first partition plate 910, the second partition plate 920 and the third partition plate 930 are inserted into their respective slots, their top surfaces are higher than the top surfaces of the partition body 510, the first partition plate 710 and the second partition plate 720, but lower than the top surface of the housing 100.

[0149] In some embodiments, vertically arranged sliding slots are provided on both buffer partitions 500.

[0150] Specifically, there are two sliding slots, which are arranged on both sides of the buffer partition. Each sliding slot is opened from top to bottom along the height direction of the buffer partition 500.

[0151] When sealing, the first baffle plate 910 can be inserted vertically into the buffer baffle 500 along the sliding slot to fit the outer side of the buffer baffle 500, so as to achieve the baffle sealing of the baffle hole on the buffer baffle 500. This sealing method has a better sealing effect on the buffer baffle. Its fit to the outer side of the buffer baffle 500 can avoid the interference of internal deposits on its sealing.

[0152] In some embodiments, the cover module 940 includes a plurality of sub-covers 941, which are respectively disposed on top of a plurality of sub-culture chambers 121 to seal the sub-culture chambers 121.

[0153] In some embodiments, a detection probe 950 is also provided on the cover body 941 for detection.

[0154] In some embodiments, the cover module 940 is a cover that simultaneously covers and seals the tops of the multiple sub-culture chambers 121.

[0155] A sealing strip can be installed at the contact point between the cover and the side wall of the box to ensure a tight seal against the box wall.

[0156] When it is necessary to test the respiratory metabolic rate of buried shellfish, the first experimental mode can be activated, and the breeding chamber can be sealed by the baffle assembly and the cover module 940.

[0157] When it is necessary to cultivate burrowing shellfish in a simulated actual growth environment, the baffle assembly and cover module 940 can be removed to ensure that water can flow normally into each sub-cultivation chamber for cultivation.

[0158] In some embodiments of this application, a control culture container 800 is also provided on the side of the box 100, and a control culture space is formed inside the control culture container 800.

[0159] To simultaneously achieve a comparison with the seawater tidal aquaculture group, a control aquaculture container 800 is set on the side of the tank 100.

[0160] When setting up the control culture container 800, water can be artificially added to it for culture, with all other conditions being the same, to serve as a control for the burrowing shellfish cultured in the culture chamber 120 located below.

[0161] Alternatively, all other conditions can be the same, and no sediment can be placed inside.

[0162] In this embodiment, the burial shellfish physiological experimental device can also be used to cultivate the control group, which allows the experimenter to simultaneously detect shellfish parameters in both the control and experimental groups, thereby improving the efficiency and accuracy of the experiment.

[0163] This embodiment also proposes a method for culturing burrowing shellfish using a physiological experimental device for burrowing shellfish, which includes the following steps:

[0164] Place a 600mm aquaculture cage into a 120mm aquaculture chamber;

[0165] Lay sediment inside the aquaculture cage 600, making it lower than or flush with the top surface of the aquaculture cage 600;

[0166] Laying the sediment after the aquaculture cage 600 is in place can prevent the sediment from falling off if it is laid in advance in the aquaculture cage 600.

[0167] Open the inlet pipe 200, close the second outlet pipe 400, and open the first outlet pipe 300;

[0168] Water is injected into the tank 100 at a certain flow rate. When the water level reaches the first preset height, burrowing shellfish are released into the aquaculture net cage 600.

[0169] The first preset time for raising burrowing shellfish within a 100-meter enclosure;

[0170] In the initial breeding stage, first fill the container 100 with water. When filling the container, the water inlet pipe 200 can be filled with water at the maximum flow rate or a medium flow rate.

[0171] The first preset height position is that the water level inside the tank 100 is at least higher than the height of the sediment, so as to ensure that the water can submerge the sediment, thereby ensuring that the buried shellfish can grow in a watery environment.

[0172] After filling the tank 100 with water, the burrowing shellfish can be placed inside the aquaculture cage 600, where they can automatically burrow into the sediment.

[0173] In the initial stage of aquaculture, water can be kept in the 600-meter-wide aquaculture cages to allow the buried shellfish to grow in this environment for a predetermined period of time, enabling them to adapt to their living environment in advance.

[0174] The initial preset time can be 3-5 days.

[0175] After 3-5 days of burrowing shellfish, the flow rate of the inlet pipe 200 can be controlled by the controller to alternate between high and low within a certain flow range until the water level in the tank 100 is level with the first outlet pipe 300.

[0176] The flow rate ranges from 5 to 50 L / h. Water can be introduced through the inlet pipe at varying flow rates to simulate the tidal environment of seawater.

[0177] For example, during setup, the initial flow rate of the inlet pipe 200 can be 5 L / h, then change to 15 L / h, then to 30 L / h, then to 45 L / h, then to 30 L / h, and then back to 15 L / h, alternating between high and low flow rates to simulate seawater.

[0178] In some embodiments of this application, after 5-6 hours, the water level in the tank 100 will reach the same level as the first water outlet pipe 300. When the water level in the tank 100 is the same as the first water outlet pipe 300, it means that the water level in the tank 100 has reached stability.

[0179] When the water level in the tank 100 reaches the same level as the first outlet pipe 300, the inlet pipe 200 is controlled to be open for a second preset time.

[0180] When the water level reaches a stable state, the inlet pipe 200 is normally open and the first outlet pipe 300 is normally open. Water will enter through the inlet pipe 200 and then continuously flow out through the first outlet pipe 300. The second preset time is 1-2 hours, simulating the stabilization time after the tide has risen.

[0181] Then, control the inlet pipe 200 to close and the second outlet pipe 400 to open, and control the flow rate of the second outlet pipe 400 to alternate between high and low within a certain flow range until all the water in the tank 100 is discharged.

[0182] The second water outlet pipe 400 is opened, at which point the water inside the tank 100 is continuously released.

[0183] By controlling the flow rate of the second outlet pipe 400, it can simulate the low tide environment at sea.

[0184] After the second water outlet pipe 400 is opened, the water inside the tank 100 will be completely drained in about 5-6 hours, which is equivalent to the receding tide.

[0185] The system controls the inlet pipe 200 to remain closed, and the first outlet pipe 300 and the second outlet pipe 400 to remain open for a third preset time. After the water in the tank 100 is completely drained, the system simulates the time period after the tide recedes.

[0186] The third preset time is 1-2 hours.

[0187] By effectively controlling the flow rate and water inlet time of the inlet pipe 200, the maintenance time for the water level inside the tank 100 to reach the height of the first outlet pipe 300, the flow rate and water outlet time of the second outlet pipe 400, and the maintenance time after the tank 100 is drained, the growth environment of the burrowing shellfish placed in the culture chamber 120 is close to that of the marine growth environment. This ensures that the parameters of the cultured burrowing shellfish are similar to those of the burrowing shellfish cultured in the sea, thereby ensuring the accuracy of the measured parameters.

[0188] This embodiment also proposes an experimental method for studying burrowing mollusks using the aforementioned physiological experimental apparatus, comprising the following steps:

[0189] Placement of burrowing shellfish:

[0190] Multiple aquaculture net cages, each with a capacity of 600, were placed into multiple burial shellfish physiological experimental devices.

[0191] Sediment was laid inside multiple aquaculture cages, each 600 cubic meters in size.

[0192] Open the inlet pipe 200 and the first outlet pipe 300 of each burrowing shellfish physiological experimental device, close the second inlet pipe 200, and control the inlet pipe 200 to inject water into the tank 100 at the maximum flow rate so that the water level is at least higher than the height of the sediment.

[0193] The same number and size of burrowing shellfish were placed in multiple aquaculture cages totaling 600.

[0194] By pre-filling multiple tanks with water, a burrowing environment for shellfish growth is created.

[0195] Water injection aquaculture: Multiple inlet pipes 200 of multiple burial shellfish physiological experimental devices are controlled to inject water from the tank 100 to the level of the corresponding first outlet pipe 300 in a variable flow rate manner. The time required for each burial shellfish physiological experimental device is the water injection time, and the water injection time of multiple burial shellfish physiological experimental devices increases in an incremental manner.

[0196] After the position inside the tank 100 is flush with the first water outlet pipe 300, the multiple water inlet pipes 200 are kept open for the first holding time.

[0197] In the water-filled aquaculture, water is injected into the box 100 of each burrowing shellfish physiological experimental device through the water inlet pipe 200. The flow rate of the water inlet pipe 200 in each device is set differently so that the water injection time taken for the water level in the box 100 of each device to reach the height of the first water outlet pipe 300 is different.

[0198] The water injection time of multiple burrowing shellfish physiological experimental devices was increased sequentially.

[0199] Drainage culture: The second outlet pipe 400 of multiple burial shellfish physiological experimental devices is controlled to drain the water in the tank 100 in a variable flow manner. The time required for each burial shellfish physiological experimental device is the drainage time, and the drainage time of multiple burial shellfish physiological experimental devices decreases in sequence.

[0200] After the water in the tank 100 is drained, keep multiple water inlet pipes 200 closed and the second water outlet pipe 400 open for a second holding time;

[0201] The alternating cycle of water-filled and water-drained aquaculture was controlled, and after a preset aquaculture period, multiple aquaculture cages 600 were removed to extract growth parameters of the burrowing shellfish in the cages 600.

[0202] In some embodiments of this application, four physiological experimental devices for burrowing shellfish are set up, and the water filling time for the four devices is 4 hours, 6 hours, 8 hours and 10 hours respectively.

[0203] The drainage times for the four devices are 8 hours, 6 hours, 4 hours, and 2 hours, respectively.

[0204] By setting varying water injection and drainage times, the time spent in seawater by the burrowing shellfish cultured inside is differentiated.

[0205] For burrowed shellfish cultured at different high and low tide times, their growth performance parameters can be measured after the culture is completed.

[0206] For example, parameters such as the weight, shell length, and shell thickness of burrowing shellfish can be measured to obtain the most suitable high tide and low tide times for burrowing shellfish.

[0207] The physiological experimental devices for burrowing shellfish in this embodiment showed that the growth parameters of burrowing shellfish were optimal when the water injection time (high tide time) was 10 hours and the drainage time (low tide time) was 2 hours, providing guidance for the cultivation of burrowing shellfish.

[0208] Therefore, when cultivating burrowing shellfish on the beach, they can be released into medium-deep areas of the beach to ensure that the shellfish spend a longer time in the seawater and grow better.

[0209] In some embodiments of this application, the experimental methods also include methods for measuring the respiratory metabolic rate of burrowing mollusks:

[0210] After a pre-set breeding period, the first partition plate, the second partition plate, and the third partition plate in the physiological experimental device for burial shellfish with burial shellfish are respectively inserted into the first slot, the second slot, and the third slot. The cover module is placed on top of the sub-breeding chamber to seal the multiple sub-breeding chambers.

[0211] At the same time, a control burrowing shellfish physiological experiment device was set up and sealed, and no burrowing shellfish were placed inside it;

[0212] After a preset time period, the dissolved oxygen content and ammonia nitrogen concentration in the seawater of the burial shellfish physiological experimental device were measured. The respiratory metabolic rate of the burial shellfish was obtained based on the dissolved oxygen content and ammonia nitrogen concentration in the control burial shellfish physiological experimental device and the dissolved oxygen content and ammonia nitrogen concentration in the burial shellfish physiological experimental device.

[0213] The control apparatus for the physiological experiment of burrowing shellfish is exactly the same as the above apparatus in terms of structure and sealing method, except that no burrowing shellfish are placed inside it;

[0214] After a preset time period, the dissolved oxygen content and ammonia nitrogen concentration in the seawater of the burial shellfish physiological experimental device are measured. The preset time can be 2-4 hours.

[0215] The respiratory metabolic rate of burrowing shellfish was obtained by comparing the dissolved oxygen and ammonia nitrogen concentrations in seawater in a control burrowing shellfish physiological experiment setup with those of burrowing shellfish.

[0216] The burrowing shellfish physiological experimental device in this embodiment can not only be used for the cultivation of burrowing shellfish, but also to directly measure the respiratory metabolic rate of burrowing shellfish after the cultivation is completed by sealing them. Multiple uses are achieved through one device, making it widely applicable.

[0217] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions claimed by the present invention.

Claims

1. A physiological experimental apparatus for burrowing mollusks, characterized in that, The box-shaped structure has an internal storage space. A water inlet pipe is located at the upper part of the tank, and a first flow control element is installed on the water inlet pipe; The first water outlet pipe is located at the upper part of the tank, and its height is lower than that of the water inlet pipe; The second water outlet pipe is located at the bottom of the tank, and a second flow control element is installed on the second water outlet pipe. Two buffer partitions are provided, which are respectively arranged near the water inlet pipe and the first water outlet pipe to divide the box into a buffer chamber and a breeding chamber in sequence. A breeding net cage is placed inside the breeding chamber. Multiple breeding mesh holes are formed on the wall of the breeding net cage, and sediment is laid inside the breeding net cage. The controller communicates with the first flow control element and the second flow control element, and is able to control the opening degree of the first flow control element and the second flow control element.

2. The physiological experimental apparatus for burrowing mollusks according to claim 1, characterized in that, The aquaculture cages include: The cage itself has an internal aquaculture space; And the cage handle connected to the cage body; Multiple conical protrusions are formed on the bottom of the cage body, and the multiple conical protrusions are evenly arranged at the intersection of adjacent aquaculture mesh openings.

3. The physiological experimental apparatus for burrowing mollusks according to claim 1, characterized in that, The buffer baffle includes: The partition body has multiple partition holes that are evenly distributed throughout it. A flexible buffer element, detachably connected at the top of the baffle body, has a guide surface that directs the water flow.

4. The physiological experimental apparatus for burrowing mollusks according to claim 3, characterized in that, It also includes a partition component arranged inside the breeding chamber to divide the breeding chamber into multiple sub-breeding chambers, and the breeding net cage is provided in each of the sub-breeding chambers; The separating component includes: The first partition plate is vertically arranged between the two buffer partitions, and the first partition holes are evenly distributed on the first partition plate. Two second partition plates are provided, which are perpendicular to the first partition plate and are connected and fixed to the first partition plate at one end, while the other end abuts against the side wall of the box. The second partition plate is evenly covered with second partition holes. The second partition plate, the first partition plate, the buffer partition and the side wall of the box form the sub-breeding cavity.

5. The physiological experimental apparatus for burrowing mollusks according to claim 1, characterized in that, It also includes a baffle assembly, which includes a first baffle, a second baffle, and a third baffle; And the cover module; A first slot with a top opening is provided on the body of the partition for inserting the first partition plate; The first partition plate is provided with a second slot with a top opening for inserting the second partition plate; The second partition plate is provided with a third slot with a top opening for inserting the third partition plate; The experimental apparatus for the physiological functions of burrowing mollusks includes a first experimental mode and a second experimental mode. In the first experimental mode, the first partition plate, the second partition plate and the third partition plate are respectively inserted into the first slot, the second slot and the third slot, and the cover module is placed on top of the sub-culture chamber to seal the multiple sub-culture chambers. In the second experimental mode, the first partition, the second partition and the third partition are pulled out from the first slot, the second slot and the third slot respectively, the cover module is away from the sub-breeding chamber, and the multiple sub-breeding chambers are interconnected and connected to the buffer chamber.

6. The physiological experimental apparatus for burrowing mollusks according to claim 1, characterized in that, The height of the water outlet pipe is higher than that of the buffer barrier.

7. The physiological experimental apparatus for burrowing mollusks according to claim 1, characterized in that, A control culture container is also provided on the side of the box, and a control culture space is formed inside the control culture container.

8. A method for cultivating burrowing shellfish based on the burrowing shellfish physiological experimental apparatus according to any one of claims 1-7, characterized in that, Includes the following steps: Place the aquaculture cages into the aquaculture chamber; Lay sediment inside the aquaculture cages, making it lower than or level with the top surface of the aquaculture cages; Open the inlet pipe, close the second outlet pipe, and open the first outlet pipe; Water is injected into the cage at a certain flow rate. When the water level reaches the first preset height, burrowing shellfish are released into the aquaculture cage. The first preset time for raising burrowing shellfish in the container; The controller controls the inlet water flow rate to alternate between high and low within a certain flow range until the water level in the tank is level with the first outlet water pipe. When the water level in the tank reaches the same level as the first water outlet pipe, the first water outlet pipe is kept open for a second preset time. The inlet pipe is closed and the outlet pipe is opened. The flow rate of the outlet pipe is controlled to alternate between high and low within a certain flow range until all the water in the tank is discharged. The control system keeps the inlet pipe closed, the first outlet pipe and the second outlet pipe open for a third preset time.

9. An experimental method for studying the performance parameters of burrowing mollusks based on the physiological experimental apparatus for burrowing mollusks according to any one of claims 1-7, characterized in that, Includes the following steps: Placement of burrowing shellfish: Multiple aquaculture cages were placed into multiple burial shellfish physiological experimental devices; Sediment was laid inside multiple aquaculture cages; Open the inlet pipe and the first outlet pipe of each burrowing shellfish physiological experimental device, close the second inlet pipe, and control the inlet pipe to inject water into the tank at the maximum flow rate so that the water level is at least higher than the height of the sediment. Place the same number and size of burrowing shellfish in multiple aquaculture cages; Water injection aquaculture: The water level in the tank is injected to the level of the first outlet pipe by controlling the water inlet pipe of multiple burial shellfish physiological experimental devices in a variable flow manner. The time required for each burial shellfish physiological experimental device is the water injection time, and the water injection time of multiple burial shellfish physiological experimental devices increases in an incremental manner. Inside the box water After the position reaches the level with the first water outlet pipe, keep multiple water inlet pipes open for the first holding time. Drainage culture: The second outlet pipe of multiple burial shellfish physiological experimental devices is controlled to drain the water in the tank in a variable flow manner. The time required for each burial shellfish physiological experimental device is the drainage time, and the drainage time of multiple burial shellfish physiological experimental devices varies in descending order. After the water is drained from the tank, keep multiple inlet pipes closed and the second outlet pipe open for a second holding time. The alternating cycle of water-filled and water-drained aquaculture was controlled, and multiple aquaculture cages were removed after a preset aquaculture period to extract growth parameters of the burrowing shellfish in the cages.

10. A method for determining the respiratory metabolic rate of burrowing mollusks based on the physiological experimental apparatus for burrowing mollusks as described in claim 5: After a pre-set breeding period, the first partition plate, the second partition plate, and the third partition plate in the physiological experimental device for burial shellfish with burial shellfish are respectively inserted into the first slot, the second slot, and the third slot. The cover module is placed on top of the sub-breeding chamber to seal the multiple sub-breeding chambers. At the same time, a control burrowing shellfish physiological experiment device was set up and sealed, and no burrowing shellfish were placed inside it; After a preset time period, the dissolved oxygen content and ammonia nitrogen concentration in the seawater of the burial shellfish physiological experimental device were measured. The respiratory metabolic rate of the burial shellfish was obtained based on the dissolved oxygen content and ammonia nitrogen concentration in the control burial shellfish physiological experimental device and the dissolved oxygen content and ammonia nitrogen concentration in the burial shellfish physiological experimental device.

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