Weakly fluorescent sealed collection device

Abstract

The application relates to the technical field of bio-imaging, and particularly discloses a weak fluorescence sealing collection device, which comprises a supporting unit, a fixing base and a placing plate; the upper end of the supporting unit is fixedly connected with the fixing base; a groove is arranged on the upper surface of the fixing base and rotationally connected with the placing plate; the side surface of the placing plate is transmissionally connected with a driving wheel; and the driving wheel is provided with a driving unit; after the anaesthetized biological mouse for collecting fluorescence data is placed on the upper surface of the placing plate, the driving unit is controlled to drive the placing plate to rotate one circle and stop for four times; at each time of stopping, the fluorescence CCD can collect the weak fluorescence emitted by the mouse in a dark environment, then the visible light CCD with a flash lamp is used to take a visible light photo of the biological mouse, and then the multi-angle photos after shooting can be used for three-dimensional modeling, so that the accurate fluorescence position of the biological mouse is obtained, and the problem that the existing weak fluorescence collection is exposed to the brightness space and is affected by visible light is solved.

Description

Technical Field

[0001] This invention relates to the field of bioimaging technology, specifically a weak fluorescence sealed acquisition device. Background Technology

[0002] In biofluorescence tomography, the acquisition of weak fluorescence signals is a crucial step. Only by accurately acquiring weak fluorescence data can three-dimensional models be fluorescently labeled, thereby enabling direct monitoring of cellular activities and gene behavior in living organisms. Through this in vivo imaging system, biological processes such as tumor growth and metastasis, the development of infectious diseases, and the expression of specific genes in living animals can be observed.

[0003] Chinese invention patent CN116626000A, published on August 22, 2023, discloses a microlens-based fluorescence imaging device. The device includes an expandable stage, a micro-dark chamber, an imaging cylinder, and an incident light source. The micro-dark chamber has a hole at its top center and a light-shielding cover movably mounted on its top. It also includes a cylindrical tube, microlenses, and a filter, an image sensor encapsulation cover, and an image sensor photosensitive array arranged sequentially from the bottom of the cylindrical tube downwards. This invention solves the problem of low imaging resolution in traditional lensless fluorescence imaging systems. The invention also discloses a fluorescence image acquisition method. Using the fluorescence imaging device of this invention, the method includes sequential fluorescence processing, turning on the incident light source, and adjusting the position of the microlens to acquire the fluorescence image. This invention achieves efficient fluorescence image acquisition.

[0004] However, the aforementioned publicly available solutions have the following shortcomings: the darkroom area of ​​existing fluorescence imaging devices is small, making it impossible to accommodate and fix the acquired fluorescence CCD, optical CCD, and laser within the darkroom. This makes it impossible to complete high-precision weak fluorescence acquisition, resulting in reduced imaging accuracy and reduced research effectiveness. Summary of the Invention

[0005] The purpose of this invention is to solve the above-mentioned technical problems and provide a weak fluorescence sealed collection device.

[0006] To achieve the above objectives, the present invention employs the following technical solutions; The technical solution adopted by this invention to solve its technical problem is as follows: A weak fluorescence sealed acquisition device according to this invention includes a support unit, a fixed base, and a placement plate; the upper end of the support unit is fixedly connected to the fixed base; a groove is opened on the upper surface of the fixed base and the placement plate is rotatably connected inside the groove; a drive wheel is meshed and driven to the side of the placement plate; a drive unit is provided on the drive wheel; a stepped groove is also opened on the upper surface of the fixed base; a light-shielding sleeve is sleeved on the stepped groove; multiple protruding square tubes are connected and fixedly connected to the light-shielding sleeve; a movable tube is pinned to the inside of the protruding square tubes; a filter and a limiting plate are inserted into the groove of the movable tube; a second laser, a fluorescent CCD, and a visible light CCD are inserted into the limiting plate; a sealing cover is fixedly connected to the movable tube at the position corresponding to the fluorescent CCD and the visible light CCD; a light-shielding film is sleeved between the protruding square tube and the movable tube; a light-shielding cover is sleeved on the light-shielding sleeve.

[0007] Furthermore, the drive unit includes a drive shaft and a motor; the drive shaft is fixedly connected to the hole of the drive wheel; the lower end of the drive shaft is fixedly connected to the motor; the motor is fixedly connected to the lower surface of the fixed base.

[0008] Furthermore, the support unit includes a fixed plate, a T-shaped frame, and a T-shaped sleeve; the T-shaped sleeve is fixedly connected to the lower surface of the fixed base; the lower end of the T-shaped sleeve is slotted and slidably inserted with the T-shaped frame; the lower end of the T-shaped frame is fixedly connected to the fixed plate.

[0009] Furthermore, multiple adjustment holes are evenly distributed on the T-shaped frame and T-shaped sleeve; insert plates are inserted into the adjustment holes.

[0010] Furthermore, the outer surface of the light-shielding sleeve is fixedly connected with multiple protruding plates; each of the protruding plates is rotatably connected with a rotating shaft; the upper end of the rotating shaft is threaded with a fixed sleeve; the upper end of the fixed sleeve is pinned to a support point; the support point is fixedly connected to the lower surface of the movable tube; a nut is threaded on the rotating shaft at a position corresponding to the fixed sleeve.

[0011] Furthermore, the fixed base and the lower surface of the placement plate are provided with clearance holes; a central hole is provided in the clearance holes; a support frame is fixedly connected in the clearance holes at the position corresponding to the central hole; a first laser is fixedly connected on the support frame at the position corresponding to the central hole.

[0012] The weak fluorescence sealed collection device provided by this invention has the following beneficial effects: 1. This invention involves placing an anesthetized biological mouse, after which fluorescence data is to be collected, on the surface of a placement plate. The angle of the movable tube is then adjusted so that the internal fluorescence CCD and visible light CCD are aligned with the center of the biological mouse. A second laser is then activated to project and excite fluorescence onto the biological mouse. A light-shielding cover is then fitted onto the light-shielding sleeve to complete the overall light shielding. The drive unit is controlled to drive the placement plate to rotate one revolution and pause at multiples of four. During each pause, the fluorescence CCD can collect the weak fluorescence emitted by the mouse in the dark environment. Then, in conjunction with a visible light CCD equipped with a flash, visible light photographs of the biological mouse are taken. The multi-angle photographs can then be used to create a three-dimensional model, thereby obtaining the precise fluorescence position on the biological mouse. This solves the problem of existing weak fluorescence collection methods being affected by visible light when exposed to bright spaces. 2. This invention allows the position within the fixed sleeve to be changed by rotating the rotating shaft, and then the rotating shaft can be fixed by rotating the nut. This allows for adjustment of the pitch angle of the movable tube at the end of the fixed sleeve, enabling the imaging of the center position of organisms of different sizes, and allowing for precise collection of weak fluorescence. Attached Figure Description

[0013] The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings; Figure 1 A schematic diagram of the first isometric structure of a weak fluorescence sealed acquisition device; Figure 2 A schematic diagram of the second isometric structure of a weak fluorescence sealed acquisition device; Figure 3 A schematic diagram of the first half-section structure of a weak fluorescence sealed collection device; Figure 4 This is a schematic diagram of the second half of a sealed collection device for weak fluorescence.

[0014] The following are the labels in the diagram: 1. Fixed plate; 2. T-shaped frame; 3. T-shaped sleeve; 4. Adjustment hole; 5. Insert plate; 6. Fixed base; 7. Clearance hole; 8. Placement plate; 9. Drive wheel; 10. Drive shaft; 11. Motor; 12. Center hole; 13. First laser; 14. Support frame; 15. Stepped groove; 16. Protruding square tube; 17. Movable tube; 18. Filter; 19. Second laser; 20. Limiting plate; 21. Sealing cover; 22. Light-shielding film; 23. Protruding plate; 24. Rotating shaft; 25. Fixed sleeve; 26. Support point; 27. Nut; 28. Light-shielding cover; 29. ​​Visible light CCD; 30. Fluorescent CCD; 31. Light-shielding sleeve. Detailed Implementation

[0015] It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the invention.

[0016] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] It should be noted that in the embodiments of the present invention, all directional indications (such as up-down-left-right-forward-backward...) are only used to explain the relative positional relationship and movement between the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indication will also change accordingly. The connection can be a direct connection or an indirect connection.

[0018] Please see Figure 1-4 As shown, a weak fluorescence sealed acquisition device includes a support unit, a fixed base 6, and a placement plate 8. The fixed base 6 is fixedly connected to the upper end of the support unit. The fixed base 6 has a groove on its upper surface and the placement plate 8 is rotatably connected inside the groove. The side of the placement plate 8 is meshed with a drive wheel 9. The drive wheel 9 is equipped with a drive unit. The fixed base 6 also has a stepped groove 15. A light-shielding sleeve 31 is sleeved on the stepped groove 15. Multiple protruding square tubes 16 are connected and fixedly connected to the light-shielding sleeve 31. A movable tube 17 is pinned to the inside of the protruding square tubes 16. A filter 18 and a limiting plate 20 are inserted into the slot of the movable tube 17. A second laser 19, a fluorescent CCD 30, and a visible light CCD 29 are inserted into the limiting plate 20. A sealing cover plate 21 is fixedly connected to the movable tube 17 at the positions corresponding to the fluorescent CCD 30 and the visible light CCD 29. A sleeve is fitted between the protruding square tubes 16 and the movable tube 17. A light-shielding film 22 is attached; a light-shielding cover 28 is fitted onto the light-shielding sleeve 31; during operation, anesthetized biological mice for which fluorescence data is to be collected are placed on the upper surface of the placement plate 8, and then the angle of the movable tube 17 is adjusted so that the internal fluorescent CCD 30 and visible light CCD 29 can be aligned with the center position of the biological mouse. Then, the second laser 19 is turned on to project and excite fluorescence onto the biological mouse. Then, the light-shielding cover 28 is fitted onto the light-shielding sleeve 31 to complete the overall light shielding. The control drive unit drives the placement plate 8 to rotate one revolution and pause at multiples of four. During each pause, the fluorescent CCD 30 can collect the weak fluorescence emitted by the mouse in the dark environment. Then, in conjunction with the visible light CCD 29 with a flash lamp, a visible light photograph of the biological mouse is taken. Then, a three-dimensional model can be performed on the completed multi-angle photographs to obtain the precise fluorescence position on the biological mouse, thus solving the problem that the existing weak fluorescence collection is affected by visible light when exposed to brightness space.

[0019] The drive unit includes a drive shaft 10 and a motor 11; the drive shaft 10 is fixedly connected to the hole of the drive wheel 9; the lower end of the drive shaft 10 is fixedly connected to the motor 11; the motor 11 is fixedly connected to the lower surface of the fixed base 6; during operation, the motor 11 drives the drive shaft 10 and the drive wheel 9 to rotate, and the drive wheel 9 drives the placement plate 8 to rotate through gear meshing transmission, thereby driving the organism placed in the center of the placement plate 8 to perform fluorescent photography.

[0020] The support unit includes a fixed plate 1, a T-shaped frame 2, and a T-shaped sleeve 3. The T-shaped sleeve 3 is fixedly connected to the lower surface of the fixed base 6. The lower end of the T-shaped sleeve 3 is slotted and slidably inserted with the T-shaped frame 2. The lower end of the T-shaped frame 2 is fixedly connected to the fixed plate 1. During operation, the interlocking T-shaped frame 2 and T-shaped sleeve 3 allow the distance between the fixed plate 1 and the fixed base 6 to be adjusted. With the fixed plate 1 fixed to the ground, the height of the fixed base 6 can be adjusted, making it convenient for people of different heights and in different situations to operate. Furthermore, the connection between the T-shaped frame 2 and T-shaped sleeve 3 ensures high support strength while preventing torsion and maintaining a horizontal position, thus solving the problem of non-parallelism in existing vertically adjustable structures.

[0021] Multiple adjustment holes 4 are evenly distributed on the T-shaped frame 2 and T-shaped sleeve 3; insert plates 5 are inserted into the adjustment holes 4; during operation, the position of the T-shaped sleeve 3 on the T-shaped frame 2 can be locked by inserting the insert plates 5 into the adjustment holes 4, thereby adjusting the height of the fixed base 6 fixed on the T-shaped sleeve 3, which is convenient for people of different heights and occasions to operate. Moreover, the connection between the T-shaped frame 2 and T-shaped sleeve 3 makes the support strong and not prone to torsion, always keeping it horizontal, thus solving the problem of non-parallelism in existing vertical adjustment structures.

[0022] Multiple protruding plates 23 are fixedly attached to the outer surface of the light-shielding sleeve 31; a rotating shaft 24 is rotatably connected to each of the protruding plates 23; a fixed sleeve 25 is threaded onto the upper end of the rotating shaft 24; a support point 26 is pinned to the upper end of the fixed sleeve 25; the support point 26 is fixed to the lower surface of the movable tube 17; a nut 27 is threaded onto the rotating shaft 24 corresponding to the position of the fixed sleeve 25; during operation, the position within the fixed sleeve 25 can be changed by rotating the rotating shaft 24, and then the fixed rotating shaft 24 can be fixed by rotating the nut 27, thereby adjusting the pitch angle of the movable tube 17 at the end of the fixed sleeve 25, so that the center position of organisms of different sizes can be photographed, and the collection of weak fluorescence can be carried out accurately.

[0023] The fixed base 6 and the placement plate 8 have clearance holes 7 on their lower surfaces; a central hole 12 is formed in the clearance hole 7; a support frame 14 is fixedly connected to the clearance hole 7 at the position corresponding to the central hole 12; a first laser 13 is fixedly connected to the support frame 14 at the position corresponding to the central hole 12; during operation, the clearance hole 7 and the central hole 12 allow the first laser 13 to be aligned with the center of the organism to be measured for transmission excitation, ensuring that it is always in the center position for transmission excitation, thereby improving the accuracy of fluorescence excitation and the accuracy of acquisition, and solving the problem that existing lasers are difficult to align with the center of the object.

[0024] Using the above scheme, in use, the anesthetized biological mouse for which fluorescence data is to be collected is placed on the upper surface of the placement plate 8. Then, the angle of the active tube 17 is adjusted so that the internal fluorescent CCD 30 and visible light CCD 29 are aligned with the center of the biological mouse. Then, the second laser 19 is turned on to project and excite fluorescence onto the biological mouse. Then, the light-shielding cover 28 is fitted onto the light-shielding sleeve 31 to complete the overall light shielding. The control drive unit drives the placement plate 8 to rotate one revolution and pause at multiples of four. During each pause, the fluorescent CCD 30 can collect the weak fluorescence emitted by the mouse in the dark environment. Then, in conjunction with the visible light CCD 29 with a flash, visible light photographs of the biological mouse are taken. Then, three-dimensional modeling can be performed on the multi-angle photographs to obtain... The precise fluorescence location on the biological mouse solves the problem of existing weak fluorescence collection being affected by visible light in bright spaces. The motor 11 drives the drive shaft 10 and the active wheel 9 to rotate. The active wheel 9 drives the placement plate 8 to rotate through gear meshing, thereby driving the biological sample placed in the center of the placement plate 8 to collect fluorescence images. The interlocking T-shaped frame 2 and T-shaped sleeve 3 allow the distance between the fixing plate 1 and the fixing base 6 to be adjusted. With the fixing plate 1 fixed to the ground, the height of the fixing base 6 can be adjusted, making it convenient for people of different heights and in different situations to operate. Furthermore, the connection between the T-shaped frame 2 and T-shaped sleeve 3 ensures strong support and prevents torsion, keeping the sample horizontal and solving the problem of non-parallelism in existing vertical adjustment structures.

[0025] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0026] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A weak fluorescence sealed collection device, characterized in that; The system includes a support unit, a fixed base (6), and a placement plate (8); the upper end of the support unit is fixedly connected to the fixed base (6); the upper surface of the fixed base (6) has a groove and the placement plate (8) is rotatably connected inside the groove; the side of the placement plate (8) is meshed with a drive wheel (9); the drive wheel (9) is provided with a drive unit; the upper surface of the fixed base (6) also has a stepped groove (15); a light-shielding sleeve (31) is sleeved on the stepped groove (15); a plurality of protruding square tubes (16) are connected and fixedly connected to the light-shielding sleeve (31); the protruding square tubes are... A movable tube (17) is connected to the inner tube (16) by a pin; a filter (18) and a limiting plate (20) are inserted into the movable tube (17) through a slot; a second laser (19), a fluorescent CCD (30), and a visible light CCD (29) are inserted into the limiting plate (20); a sealing cover plate (21) is fixed on the movable tube (17) at the position corresponding to the fluorescent CCD (30) and the visible light CCD (29); a light-shielding film (22) is sleeved between the protruding square tube (16) and the movable tube (17); a light-shielding cover (28) is sleeved on the light-shielding sleeve (31); The outer surface of the light-shielding sleeve (31) is fixed with a plurality of protruding plates (23); each of the protruding plates (23) is rotatably connected to a rotating shaft (24); the upper end of the rotating shaft (24) is threaded with a fixed sleeve (25); the upper end of the fixed sleeve (25) is pinned to a support point (26); the support point (26) is fixed to the lower surface of the movable tube (17); a nut (27) is threaded on the rotating shaft (24) at a position corresponding to the fixed sleeve (25). The support unit includes a fixed plate (1), a T-shaped frame (2) and a T-shaped sleeve (3); the T-shaped sleeve (3) is fixed to the lower surface of the fixed base (6); the T-shaped frame (2) is slidably inserted into the lower end of the T-shaped sleeve (3); the fixed plate (1) is fixed to the lower end of the T-shaped frame (2). Multiple adjustment holes (4) are evenly distributed on the T-shaped frame (2) and the T-shaped sleeve (3); a plate (5) is inserted into the adjustment hole (4); The fixed base (6) and the placement plate (8) have clearance holes (7) on their lower surfaces; a central hole (12) is provided in the clearance hole (7); a support frame (14) is fixedly connected in the clearance hole (7) at the position corresponding to the central hole (12); a first laser (13) is fixedly connected on the support frame (14) at the position corresponding to the central hole (12).

2. The weak fluorescence sealed acquisition device according to claim 1, characterized in that: The drive unit includes a drive shaft (10) and a motor (11); the drive shaft (10) is fixed in the hole of the drive wheel (9); the lower end of the drive shaft (10) is fixed to the motor (11); the motor (11) is fixed to the lower surface of the fixed base (6).

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