Composition for inhibiting sebum secretion and shrinking pores, as well as preparation method and application thereof

Abstract

The present invention belongs to the technical field of cosmetics, and discloses a composition for inhibiting sebum secretion and shrinking pores, as well as a preparation method and application. The composition of the present invention comprises the following components: angustifolia extract, kudzu root extract, plankton extract, olive leaf extract and medicinal pore fungus extract. The composition of the present invention is obtained by compounding angustifolia extract, kudzu root extract, plankton extract, olive leaf extract and medicinal pore fungus extract. On the basis of astringing pores, it innovatively combines the following pathways: reducing IGF-1-induced lipid synthesis, inhibiting acetyl-CoA carboxylase for de novo fatty acid synthesis, and down-regulating COX-2 gene expression. These multiple pathways work together to achieve the purpose of long-term inhibition of sebum secretion and shrinking pores.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a composition for inhibiting sebum secretion and shrinking pores, as well as a preparation method and application thereof. Background Art

[0002] With the increasingly fast-paced work and lifestyles of modern people, high stress levels, irregular work and rest schedules, and unhealthy diets, oily skin is becoming increasingly common, especially among men and adolescents. Oily skin is caused by excessive sebum secretion. Sebum is secreted by the sebaceous glands through ducts into the hair follicles, and then discharged through the pores onto the skin surface. Sebum is an oily, semi-fluid mixture containing various lipids. Its main components are triglycerides, fatty acids, phospholipids, and lipofectamine cholesterol. Normal sebum secretion forms a membrane that helps maintain the skin's barrier function, keeping it soft and moisturized. However, excessive oil secretion can easily lead to a range of skin problems, including enlarged pores, skin inflammation, and dull complexion, compromising skin health. Furthermore, chronic excessive oil production can increase the likelihood of dust and dirt sticking to the skin, leading to skin infections and acne. Therefore, to control excessive sebaceous gland secretion and reduce oil production, it is necessary to develop cosmetics with oil-control properties.

[0003] Excessive sebaceous gland secretion is often caused by an excess of androgens. Testosterone and dihydrotestosterone are both androgens. Testosterone is converted to dihydrotestosterone by the enzyme 5α-reductase. Both can promote excessive proliferation of sebaceous gland cells. Dihydrotestosterone also promotes the secretion of pro-inflammatory factors by sebaceous gland cells, further leading to skin diseases such as acne and seborrheic dermatitis. Medical treatment strategies include the use of drugs that reduce androgen secretion, such as cimetidine, and the use of finasteride, dutasteride, and epristeride to inhibit 5α-reductase. However, the use of hormone-regulating drugs has various harmful effects on the body. In particular, finasteride, dutasteride, and epristeride can affect male fertility. Therefore, the safety of existing 5α-reductase inhibitors needs to be improved.

[0004] Currently, most oil-control cosmetics on the market focus on inhibiting 5α-reductase. Although they have certain immediate effects, they do not fundamentally solve the problem and therefore cannot achieve long-lasting effects.

[0005] Patent publication number CN106361645A discloses a bio-protease acne-clearing serum. This serum contains sodium acrylate copolymer, protease, butylene glycol, niacinamide, lecithin, salicylic acid, silver oxide, and traditional Chinese medicine extracts. The addition of salicylic acid reduces oil secretion, thereby preventing excessive sebaceous gland secretion due to abnormal inflammation. However, because the active ingredient, salicylic acid, is a fruit acid, this serum can be irritating to the skin.

[0006] Therefore, it is very necessary to provide a gentle, long-lasting oil control product. Summary of the Invention

[0007] The present invention aims to address at least one of the technical problems existing in the aforementioned prior art. To this end, the present invention provides a composition for inhibiting sebum secretion and shrinking pores, as well as a preparation method and application thereof. The composition is mild and non-irritating, effectively inhibiting sebum secretion, reducing oil production, shrinking pores, achieving long-lasting oil control, and improving skin condition.

[0008] In a first aspect, the present invention provides a composition comprising the following components: angustifolia herb extract, kudzu root extract, plankton extract, olive leaf extract and medicinal facies porphyra extract.

[0009] In the composition described in the first aspect of the present invention, the effects of each component are as follows:

[0010] Epilobium angustifolium extract is derived from the aerial parts of Epilobium angustifolium. Epilobium angustifolium is a perennial, sturdy herbaceous plant of the genus Epilobium, in the family Onagraceae, native to the temperate and frigid zones of the Northern Hemisphere. Studies have shown that insulin-like growth factor-1 (IGF-1) promotes androgen synthesis in the adrenal and gonadal glands, influences androgen receptor signal transduction, stimulates sebaceous cell proliferation, promotes lipid synthesis, and induces the production of inflammatory factors. Epilobium angustifolium extract contains oenothein B and quercetin 3-O-glucuronide, which can reduce IGF-1-induced lipid synthesis.

[0011] Pueraria lobata extract is derived from the root of Pueraria lobata. Analysis of Pueraria lobata's biochemical composition revealed that the isoflavone content in Pueraria lobata is significantly higher than in other plants of the same genus. The isoflavones in Pueraria lobata extract can inhibit lipid synthesis, with puerarin, in particular, inhibiting acetyl-CoA carboxylase (ACC). ACC unidirectionally catalyzes the irreversible carboxylation of acetyl-CoA to form a substrate for fatty acid synthesis and is the rate-limiting enzyme in de novo fatty acid synthesis, thereby reducing lipid production and lowering sebaceous gland lipid secretion.

[0012] Plankton extract is derived from single-celled algae found in the Mediterranean. COX-2 is a key factor in sebum secretion and skin sensitivity. COX-2 is involved in the conversion of excessive arachidonic acid into prostaglandin E2 (PGE2) during sebaceous gland cell proliferation, leading to excessive sebum secretion and inflammation. Plankton extract regulates COX-2 gene expression, thereby controlling sebum production and inflammation at the source.

[0013] Olive leaf extract is derived from the leaves of the olive tree (Olea europaea L.), a member of the genus Oleaceae, and a Mediterranean subtropical tree native to southern Europe along the Mediterranean coast. Modern pharmacological research has demonstrated that compounds in olive leaves, such as oleuropein, hydroxytyrosol (a degradation product of oleuropein), lignans, flavonoids, and caffeoylphenylethanoid glycosides, exhibit a variety of pharmacological activities, including anti-inflammatory, antibacterial, antioxidant, antiviral, anti-tumor, and hypoglycemic and lipid-lowering activities. Oleuropein (OLE), a bitter compound with potent antioxidant properties and antibacterial properties, is absorbed and utilized by the body, downregulating IGF-1 expression and inhibiting excessive oil secretion by sebaceous glands.

[0014] The medicinal porphyrite genus belongs to the order Aphyllophorales. Its Chinese name is "Pseudo- ...

[0015] The composition described in the first aspect of the present invention is obtained by compounding an herbaceous herb extract, a kudzu root extract, a plankton extract, an olive leaf extract and a medicinal pore fungus extract. On the basis of tightening pores, it innovatively combines the reduction of IGF-1-induced lipid synthesis, the inhibition of acetyl-CoA carboxylase for de novo fatty acid synthesis, and the downregulation of COX-2 gene expression. These multiple pathways work together to achieve the purpose of long-term inhibition of sebum secretion and shrinking pores.

[0016] In some embodiments of the present invention, the composition comprises the following components, calculated by weight: 2-8 parts of Epilobium angustifolium extract, 1-5 parts of Pueraria lobata extract, 1-10 parts of plankton extract, 1-10 parts of olive leaf extract, and 3-20 parts of medicinal pore fungus extract.

[0017] In some embodiments of the present invention, the composition includes the following components, calculated by weight: 6-8 parts of Epilobium angustifolium extract, 3-5 parts of Pueraria lobata extract, 7-10 parts of plankton extract, 7-10 parts of olive leaf extract, and 14-20 parts of medicinal pore fungus extract.

[0018] In some embodiments of the present invention, the composition comprises the following components, calculated by weight: 2-5 parts of Epilobium angustifolium extract, 1-2 parts of Pueraria lobata extract, 1-6 parts of plankton extract, 1-6 parts of olive leaf extract, and 3-13 parts of medicinal pore fungus extract.

[0019] The second aspect of the present invention provides a method for preparing the composition according to the first aspect of the present invention, comprising the following steps:

[0020] The composition is obtained by mixing an herbaceous epilobium extract, a kudzu root extract, a plankton extract, an olive leaf extract and a medicinal pore mushroom extract.

[0021] In some embodiments of the present invention, the method for preparing the composition comprises the following steps:

[0022] Firstly, the epilobium angustifolia extract and the kudzu root extract are mixed with a solvent, and then the plankton extract, the olive leaf extract and the medicinal facies porphyra extract are added and mixed to obtain the composition.

[0023] In some embodiments of the present invention, the solvent is water.

[0024] The third aspect of the present invention provides a cosmetic comprising the composition described in the first aspect of the present invention.

[0025] In some embodiments of the present invention, the cosmetic further comprises cosmetically acceptable excipients; the excipients comprise at least one of an emollient, a thickener, a humectant, a pH adjuster, and a preservative.

[0026] In some embodiments of the present invention, the composition is added to the cosmetic in an amount of 0.5-10 wt %.

[0027] In some embodiments of the present invention, the composition is added to the cosmetic in an amount of 1-5 wt %.

[0028] In some embodiments of the present invention, the cosmetic comprises any one of lotion, essence, emulsion, cream, gel, mask or foundation.

[0029] In some embodiments of the present invention, the cosmetic is an essence; the essence is composed of the following components in mass percentage: 0.5-3% glycerol, 2-5% butylene glycol, 0.5-1% β-glucan, 1-2% trehalose, 0.1-0.4% polyacrylate cross-linked polymer-6, 0.02-0.1% xanthan gum, 0.02-0.05% EDTA-2Na, 1-5% of the composition described in the first aspect of the present invention, an appropriate amount of preservative, and the balance is water.

[0030] It can be understood that an appropriate amount of preservatives means that the amount added does not exceed the relevant regulations on cosmetics based on the specific type of preservatives added.

[0031] In some embodiments of the present invention, the method for preparing the essence comprises the following steps:

[0032] First, water, glycerin, and butylene glycol are mixed, heated to 80-85°C, kept constant for 15-30 minutes, and then cooled to 40-45°C; then, β-glucan, trehalose, polyacrylate cross-linked polymer-6, xanthan gum, and EDTA-2Na are added, stirred for 20-30 minutes, and mixed; finally, the temperature is cooled to 40°C, and the composition described in the first aspect of the present invention and a preservative are added, stirred and dispersed, and an essence is obtained.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] The components of the composition of the present invention are derived from natural plants, fungi and algae, do not contain hormone drugs and salicylic acid, have no side effects, are mild and non-irritating, and the combination of angustifolia extract, kudzu root extract, plankton extract, olive leaf extract and medicinal pore fungus extract exerts a synergistic effect, can inhibit sebum secretion and shrink pores in a long-lasting manner; in addition, the preparation method of the composition of the present invention is simple and suitable for industrial mass production. DETAILED DESCRIPTION

[0035] The present invention is further described in detail below by way of specific examples. Unless otherwise specified, the raw materials, reagents, or devices used in the examples are all commercially available or can be obtained by conventional methods. Unless otherwise specified, all experiments or testing methods are conventional methods in the art.

[0036] In the following examples, comparative examples, application examples, and comparative application examples, the raw materials used are all conventional commercially available products. For example, Epilobium angustifolium extract (product model: ), medicinal phytomycetes extract (product model: LS8865) can be purchased from Foshan Xin'an Company; Pueraria lobata extract (product model: ACC-Pure) can be purchased from Miaosen Co., Ltd.; plankton extract (product model: Care) can be purchased from Guangzhou Aoxue Chemical; olive leaf extract (product model: Olive leaf extract) can be purchased from Xi'an Green Sky Biotechnology Co., Ltd.

[0037] Example 1

[0038] A composition comprising the following components in parts by weight:

[0039] 2 parts of Epilobium angustifolium extract, 1 part of Pueraria lobata extract, 1 part of plankton extract, 1 part of olive leaf extract, 3 parts of medicinal pore fungus extract and 92 parts of water.

[0040] The preparation method of the above composition is as follows:

[0041] According to the above ratio, angustifolia extract and kudzu root extract are added to water and mixed evenly, and then plankton extract, olive leaf extract and medicinal phyllophora extract are added and mixed evenly to prepare a composition.

[0042] Example 2

[0043] A composition comprising the following components in parts by weight:

[0044] 8 parts of Epilobium angustifolium extract, 5 parts of Pueraria lobata extract, 10 parts of plankton extract, 10 parts of olive leaf extract, 20 parts of medicinal phytomycetes extract and 47 parts of water.

[0045] The preparation method of the above composition is as follows:

[0046] According to the above ratio, angustifolia extract and kudzu root extract are added to water and mixed evenly, and then plankton extract, olive leaf extract and medicinal phyllophora extract are added and mixed evenly to prepare a composition.

[0047] Example 3

[0048] A composition comprising the following components in parts by weight:

[0049] 6 parts of Epilobium angustifolium extract, 3 parts of Pueraria lobata extract, 8 parts of plankton extract, 7 parts of olive leaf extract, 15 parts of medicinal phytomycetes extract and 61 parts of water.

[0050] The preparation method of the above composition is as follows:

[0051] According to the above ratio, angustifolia extract and kudzu root extract are added to water and mixed evenly, and then plankton extract, olive leaf extract and medicinal phyllophora extract are added and mixed evenly to prepare a composition.

[0052] Example 4

[0053] A composition comprising the following components in parts by weight:

[0054] 7 parts of Epilobium angustifolium extract, 4 parts of Pueraria lobata extract, 7 parts of plankton extract, 7 parts of olive leaf extract, 14 parts of medicinal pore fungus extract and 61 parts of water.

[0055] The preparation method of the above composition is as follows:

[0056] According to the above ratio, angustifolia extract and kudzu root extract are added to water and mixed evenly, and then plankton extract, olive leaf extract and medicinal phyllophora extract are added and mixed evenly to prepare a composition.

[0057] Example 5

[0058] A composition comprising the following components in parts by weight:

[0059] 5 parts of Epilobium angustifolium extract, 3 parts of Pueraria lobata extract, 4 parts of plankton extract, 5 parts of olive leaf extract, 10 parts of medicinal pore fungus extract and 73 parts of water.

[0060] The preparation method of the above composition is as follows:

[0061] According to the above ratio, angustifolia extract and kudzu root extract are added to water and mixed evenly, and then plankton extract, olive leaf extract and medicinal phyllophora extract are added and mixed evenly to prepare a composition.

[0062] Comparative Example 1

[0063] The only difference from Example 3 is that the composition provided in this comparative example contains one active ingredient; specifically, it is composed of the following components in parts by mass: 39 parts of plankton extract and 61 parts of deionized water.

[0064] Comparative Example 2

[0065] The only difference from Example 3 is that the composition provided in this comparative example contains one active ingredient; specifically, it is composed of the following components in parts by mass: 39 parts of Epilobium angustifolium extract and 61 parts of deionized water.

[0066] Comparative Example 3

[0067] The only difference from Example 3 is that the composition provided in this comparative example contains one active ingredient; specifically, it is composed of the following components in parts by mass: 39 parts of olive leaf extract and 61 parts of deionized water.

[0068] Comparative Example 4

[0069] The only difference from Example 3 is that the composition provided in this comparative example contains one active ingredient; specifically, it is composed of the following components in parts by mass: 39 parts of medicinal pore fungus extract and 61 parts of deionized water.

[0070] Comparative Example 5

[0071] The only difference from Example 3 is that the composition provided in this comparative example contains one active ingredient; specifically, it is composed of the following components in parts by mass: 39 parts of Pueraria lobata extract and 61 parts of deionized water.

[0072] Comparative Example 6

[0073] The only difference from Example 3 is that the composition provided in this comparative example does not contain Pueraria lobata extract. The missing amount is added to the remaining four components in proportion to the mass ratio of the four components in Example 3. Specifically, the composition is composed of the following components in parts by mass: 6.5 parts of Epilobium angustifolium extract, 8.67 parts of plankton extract, 7.58 parts of olive leaf extract, 16.25 parts of medicinal pore fungus extract, and 61 parts of water.

[0074] Comparative Example 7

[0075] The only difference from Example 3 is that the composition provided in this comparative example does not contain the medicinal porphyromonas extract. The missing amount is added to the remaining four components in proportion to the mass ratio of the four components in Example 3. Specifically, the composition is composed of the following components in parts by mass: 9.75 parts of angustifolia extract, 4.875 parts of kudzu root extract, 13 parts of plankton extract, 11.375 parts of olive leaf extract, and 61 parts of water.

[0076] Comparative Example 8

[0077] The only difference from Example 3 is that the composition provided in this comparative example does not contain olive leaf extract. The missing amount is added to the remaining four components in proportion to the mass ratio of the four components in Example 3. Specifically, the composition is composed of the following components in parts by mass: 7.31 parts of Epilobium angustifolium extract, 3.66 parts of Pueraria lobata extract, 9.75 parts of plankton extract, 18.28 parts of medicinal pore fungus extract, and 61 parts of water.

[0078] Comparative Example 9

[0079] The only difference from Example 3 is that the composition provided in this comparative example does not contain Epilobium angustifolium extract. The missing amount is added proportionally to the remaining four components according to the mass ratio of the four components in Example 3. Specifically, the composition is composed of the following components in parts by mass: 3.55 parts of Pueraria lobata extract, 9.45 parts of plankton extract, 8.27 parts of olive leaf extract, 17.73 parts of medicinal pore fungus extract, and 61 parts of water.

[0080] Comparative Example 10

[0081] The only difference from Example 3 is that the composition provided in this comparative example does not contain plankton extract. The missing amount is added to the remaining four components in proportion to the mass ratio of the four components in Example 3. Specifically, the composition is composed of the following components in parts by mass: 7.55 parts of Epilobium angustifolium extract, 3.77 parts of Pueraria lobata extract, 8.81 parts of Olea europaea leaf extract, 18.87 parts of Familiar Fungus extract, and 61 parts of water.

[0082] Experimental Example 1 Chicken embryo hair follicle stimulation test

[0083] Taking advantage of the fact that the vascular system of the chorioallantoic membrane of mid-stage incubated chicken embryos is intact, clear and transparent, a certain amount of the test substance is directly exposed to the chorioallantoic membrane of the chicken embryo. After a period of exposure, the changes in the toxic effect indicators of the chorioallantoic membrane (such as bleeding, coagulation and vascular melting) are observed. These indicators reflect the changes in the morphology, structure, color and permeability of the blood vessels and vascular network, as well as the phenomena such as chorioallantoic membrane protein denaturation and the degree of damage. The scores are then combined to obtain a score for evaluating the eye irritation of the test substance.

[0084] The experimental method was based on the method in SNT2329-2009, using the reaction time method and the irritation score method. The eye irritation of the test substance was classified according to Table 1 based on the calculated IS value.

[0085] Table 1 Evaluation results of stimulation scoring method

[0086] Stimulus score Irritation rating IS<1 Non-irritating 1≤IS<5 Mild irritation 5≤IS<9 Moderate irritation IS≥10 Strong irritant / corrosive

[0087] Experimental results: Chicken embryo hair follicle experiments were conducted on the compositions provided in Examples 1-5 and Comparative Examples 1-10 of the present invention. The results showed that the irritation ratings of the compositions of Examples 1-5 and Comparative Examples 1-10 were all non-irritating, indicating that the components selected from the compositions of the present invention are mild and non-irritating.

[0088] Experimental Example 2 Sebum Level (CSL) Test

[0089] 75 volunteers aged 18-40 years, both male and female, were selected. The screening criteria were: sebum level ≥ 150 μg / cm3 three hours after cleansing in the morning 2 The participants were randomly divided into 15 groups. After cleansing their faces, each group took a soybean-sized sample of the compositions provided in Examples 1-5 and the compositions provided in Comparative Examples 1-10 and applied it to their foreheads. The samples were gently patted for absorption. This was done once daily, morning and evening. A sebum analyzer was used to measure sebum levels (CSL) in the fourth week and three days after discontinuation of use. The average of the user test results was taken, with the sebum level 30 minutes after cleansing the test area as the initial value. The test results are shown in Table 2.

[0090] Table 2 Sebum level test results

[0091] sample Degree of reduction in sebum levels Reduction in sebum levels after 3 days of discontinuation Example 1 -23.35％ -22.18％ Example 2 -32.27％ -30.65％ Example 3 -32.48％ -30.85％ Example 4 -31.82％ -30.23％ Example 5 -27.37％ -26.00％ Comparative Example 1 -3.55％ -0.10％ Comparative Example 2 -5.64％ -0.28％ Comparative Example 3 -6.77％ -0.20％ Comparative Example 4 -2.64％ -0.08％ Comparative Example 5 -8.13％ -2.51％ Comparative Example 6 -13.54％ -7.44％ Comparative Example 7 -16.23％ -8.83％ Comparative Example 8 -14.37％ -7.57％ Comparative Example 9 -12.54％ -6.52％ Comparative Example 10 -15.22％ -7.91％

[0092] As can be seen from Table 2, the compositions provided in Examples 1-5 and the compositions provided in Comparative Examples 1-10 both have an effect on lowering sebum levels. However, the degree of sebum reduction in the compositions provided in Examples 1-5 is between -20% and -33%, which is significantly higher than the degree of oil secretion inhibition of the compositions provided in Comparative Examples 1-10, which contain a single component or lack any active ingredient. This indicates that in the compositions provided by the present invention, the ratio of the components is scientific and reasonable, which has a synergistic effect and can effectively inhibit oil secretion.

[0093] Experimental Example 3: Number of Active Pores (NAP) and Skin Surface Sebum Percentage (SSP) Test

[0094] 75 volunteers aged 18-40 years, both male and female, were selected. The screening criteria were: sebum level ≥ 150 μg / cm3 three hours after cleansing in the morning 2 . The subjects were randomly divided into 15 groups. After cleaning the face, each group took a soybean-sized sample of the composition provided in Examples 1-5 and the composition provided in Comparative Examples 1-10 and applied it to the forehead. They were patted for absorption and used once a day in the morning and evening. The active skin surface analysis system was used to test the number of active pores (NAP) and the skin surface sebum percentage (SSP) in the fourth week and after 7 days of discontinuation. The average value of the user test results was taken, and the number of active pores (NAP) and the skin surface sebum percentage (SSP) 30 minutes after cleaning the test area were used as the initial values. The test results are shown in Table 3.

[0095] Table 3 Test results of number of active pores (NAP) and skin surface sebum percentage (SSP)

[0096]

[0097] As can be seen from the test results in Table 3, each of the Examples and Comparative Examples can reduce the number of active pores and the percentage of sebum on the skin surface. Relatively speaking, the compositions provided by Examples 1-5 have a more pronounced effect in reducing the number of active pores and the percentage of sebum on the skin surface, and can continue to maintain a good effect of inhibiting sebum secretion and shrinking pores for a certain period of time after discontinuation of the drug. This shows that the oil control effect of the composition of the present invention is stronger than that of a single component or a composition lacking any active ingredient. This shows that the active ingredients in the composition of the present invention cooperate with each other to achieve synergistic effects, which has a good effect of inhibiting sebum secretion and shrinking pores, and this effect can be maintained for a certain period of time.

[0098] Application Examples 1-5

[0099] Application Examples 1-5 provide an essence respectively. The specific formulas of the essences provided in Application Examples 1-5 and the essence provided in the blank control group are shown in Table 4 below; the component units are mass percentages.

[0100] Table 4 Essence formula

[0101] Components Application Example 1 Application Example 2 Application Example 3 Application Example 4 Application Example 5 Blank control group glycerin 3 3 3 3 3 3 Butanediol 4 4 4 4 4 4 β-glucan 1 1 1 1 1 1 Trehalose 1 1 1 1 1 1 Polyacrylate Crosspolymer-6 0.3 0.3 0.3 0.3 0.3 0.3 Xanthan gum 0.05 0.05 0.05 0.05 0.05 0.05 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Composition of Example 3 1 2 3 4 5 0 preservative appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount water to 100 to 100 to 100 to 100 to 100 to 100

[0102] The essence provided in Application Examples 1-5 is prepared as follows:

[0103] First, water, glycerin, and butylene glycol were mixed, heated to 80°C, kept constant for 15 minutes, and then cooled to 40°C; then, β-glucan, trehalose, polyacrylate cross-linked polymer-6, xanthan gum, and EDTA-2Na were added, stirred for 20 minutes, and mixed evenly; finally, the temperature was lowered to 40°C, and the composition of Example 3 and a preservative were added, stirred and dispersed to obtain an essence.

[0104] Comparative Application Example 1

[0105] The only difference from Application Example 3 is that the essence provided in this comparative application example replaces the composition of Example 3 with the composition of Comparative Example 8 in equal amounts, and the other components, contents and preparation methods remain unchanged.

[0106] Comparative Application Example 2

[0107] The only difference from Application Example 3 is that the essence provided in this comparative application example replaces the composition of Example 3 with the composition of Comparative Example 10 in equal amounts, and the other components, contents and preparation methods remain unchanged.

[0108] Experimental Example 4: Product Safety Testing

[0109] Thirty healthy subjects aged 25-50 years with no history of skin allergies were selected. The patch test method was as follows: a qualified patch tester was selected, and a closed patch test method was used. Approximately 20 mg of the essence of Application Examples 1-5 of the present invention was dropped into the patch tester. The patch was then covered on the back of the subject with a special adhesive tape. The essence of Application Examples 1-5 was applied to the tester, and the test substance was removed after 24 hours. The skin reaction was observed at 0.5, 6, 12, 24, and 48 hours after removal. The results were recorded according to the skin reaction grading standard in the "Cosmetic Hygiene Standards".

[0110] Test results: The results of the human skin patch test showed that all subjects passed the patch test and observed skin reactions at 0.5, 6, 12, 24, and 48 hours. Among them, no case showed adverse reactions such as skin erythema, papules, and blisters, indicating that the essence of the present invention is safe and non-irritating to use.

[0111] Experimental Example 5: Product Stability Test

[0112] Stability tests were performed on the essences of Example 1-5, the blank control group, and the comparative application example 1-2.

[0113] Centrifugation test: Weigh an appropriate amount of product into a centrifuge tube, centrifuge at 3000 rpm for 30 minutes, and observe the changes in appearance.

[0114] Cold resistance test: Take an appropriate amount of product, place it in a weighing bottle, seal it, and put it in a -18℃ refrigerator. Take samples every 7 days and observe the changes in appearance for 3 consecutive months.

[0115] Heat resistance test: Take an appropriate amount of product, place it in a weighing bottle, seal it, and put it in a 48℃ oven. Take samples every 7 days for observation. Continue to observe for 3 months and observe the changes in appearance.

[0116] The test results show that the essences of Application Examples 1-5, the blank control group, and Comparative Application Examples 1-2 did not separate into layers after centrifugation, high temperature, and low temperature tests, and their appearance did not change, indicating that the matrix of the cosmetics of the present invention is reasonably selected and has no adverse effect on the stability of the product.

[0117] Experimental Example 6: Product Trial Evaluation

[0118] To evaluate the effectiveness of the compositions of the present invention, the essences from Application Examples 1-5, a blank control group, and Comparative Application Examples 1-2 were used as test samples. A sensory evaluation test was conducted on 80 volunteers with oily skin, divided into 8 groups of 10 participants each. The test items included the product's oil-control effect, pore-tightening effect, mildness, and overall evaluation. The test period was 5 weeks, with the product used for the first 4 weeks and then discontinued. The test value represents the number of volunteers who approved of the corresponding product. The specific test results are shown in Table 5.

[0119] Table 5 Product trial evaluation results

[0120] sample Oil control effect after 4 weeks Pore ​​tightening effect after 4 weeks Oil control effect after 5 weeks Mildness Overall evaluation Application Example 1 7 8 8 10 8 Application Example 2 8 9 8 10 9 Application Example 3 9 10 9 10 10 Application Example 4 8 9 8 10 9 Application Example 5 9 9 9 10 9 Blank control group 1 2 1 10 2 Comparative Application Example 1 5 6 4 10 6 Comparative Application Example 2 6 4 4 10 5

[0121] The test results in Table 5 indicate that the serums of Application Examples 1-5 received significantly higher consumer approval. This indicates that the active ingredients in the composition of the present invention cooperate with each other to produce a synergistic effect, regulating skin oil secretion by tightening pores, reducing IGF-1-induced lipid synthesis, inhibiting acetyl-CoA carboxylase, which is involved in de novo fatty acid synthesis, and downregulating COX-2 gene expression, thereby achieving long-lasting sebum suppression and pore shrinkage.

[0122] The above specifically describes the preferred embodiments of the present invention, but the invention is not limited to the embodiments. Those skilled in the art may make various equivalent modifications or substitutions without violating the spirit of the present invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A composition, characterized in that The composition comprises the following components in parts by mass: 2-8 parts of angustifolia extract, 1-5 parts of kudzu root extract, 1-10 parts of plankton extract, 1-10 parts of olive leaf extract, and 3-20 parts of medicinal facies porcellus extract.

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by mass: 6-8 parts of angustifolia extract, 3-5 parts of kudzu root extract, 7-10 parts of plankton extract, 7-10 parts of olive leaf extract, and 14-20 parts of medicinal facies porcellus extract.

3. The composition according to claim 1, characterized in that The composition comprises the following components in parts by mass: 2-5 parts of angustifolia extract, 1-2 parts of kudzu root extract, 1-6 parts of plankton extract, 1-6 parts of olive leaf extract, and 3-13 parts of medicinal facies porcellus extract.

4. The method for preparing the composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: The composition is obtained by mixing an herbaceous epilobium extract, a kudzu root extract, a plankton extract, an olive leaf extract and a medicinal pore mushroom extract.

5. The preparation method according to claim 4, characterized in that The following steps are involved: Firstly, the epilobium angustifolia extract and the kudzu root extract are mixed with a solvent, and then the plankton extract, the olive leaf extract and the medicinal facies porphyra extract are added and mixed to obtain the composition.

6. A cosmetic, characterized in that: The composition comprises the composition according to any one of claims 1 to 3.

7. The cosmetic according to claim 6, characterized in that The addition amount of the composition is 0.5-10 wt%.

8. The cosmetic according to claim 7, characterized in that The addition amount of the composition is 1-5 wt%.

9. The cosmetic according to claim 6, characterized in that The cosmetics include any one of lotion, essence, emulsion, cream, gel, facial mask or foundation.

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