Preparation of Brasenia schrenkiana extract and its application in cosmetics

Abstract

The invention relates to the preparation of a water shield extract and application in cosmetics. The preparation method is as follows: fresh water shield is cleaned and crushed, and then water and a composite enzyme are added for enzymolysis to obtain an enzymolysis product; a fermentation agent is added to the enzymolysis product for fermentation treatment to obtain a fermentation product; the fermentation product is subjected to ultrasonic treatment, and then taken out and put into a grinder for grinding to obtain a slurry; the slurry is filtered to remove solid impurities in the slurry, and then the remaining liquid is put into a vacuum concentration evaporator for vacuum concentration to obtain the water shield extract; the water shield extract is first subjected to enzymolysis, and then fermented, ultrasonically treated and ground, so that the functional components in the water shield can be more fully extracted, and the prepared water shield extract has good sunburn repairing effect, and the water shield extract is added into cosmetics with sunburn repairing effect to significantly alleviate and accelerate the recovery of skin after sun exposure.

Description

Technical Field

[0001] The invention relates to the technical field of plant extraction, and in particular to the preparation of a Brasenia schrenkiana extract and the application of the extract in cosmetics. Background Art

[0002] Brasenia schreberi is a perennial aquatic herb in the genus Brasenia of the family Brasenaceae. It has a small rhizome, a slender stem with many branches; the leaves are dimorphic, the floating leaves are shield-shaped, green on the upper side, purple on the lower side, and hairless; the flowers are small, dark purple, with linear anthers; the fruits are leathery, oblong-ovate, and have curved thorns on the top. The characteristic of Brasenia schreberi is that the edible part of the tender shoots and petioles of young leaves are covered with transparent colloid, with a crystal clear appearance and smooth taste. Among the various ingredients contained in Brasenia schreberi, it has a high nutritional value and medicinal value, and is deeply loved by people, so it has become a good choice for banquets.

[0003] At present, there are many related reports on extracting the material components in Brasenia schrenkiana and using them in the field of cosmetics and health care. For example, an invention patent with application number CN201510336166.5 discloses a Brasenia schrenkiana facial mask liquid, which includes Brasenia schrenkiana water extract; licorice extract can also be added thereto; the Brasenia schrenkiana water extract is obtained by extracting Brasenia schrenkiana dry powder or freshly crushed Brasenia schrenkiana with water, centrifuging, decolorizing, removing tannins and other impurities, and then concentrating. The present invention uses Brasenia schrenkiana water extract to prepare a facial mask liquid, which can effectively inhibit the activity of elastase, collagenase, hyaluronidase, and tyrosinase. It also has a significant inhibitory effect, has the effects of increasing skin elasticity, tightening skin, delaying skin aging and whitening, has little irritation to the skin, has good skin affinity, and is healthy and environmentally friendly.

[0004] However, in the prior art, the preparation of water shield extract mostly adopts the water extraction method, that is, the water shield is placed in water for heating extraction, and the water shield leaves are taken out after a period of time. Although this method is simple to operate, it cannot fully extract the material components in the water shield, thereby limiting the application of the water shield extract.

[0005] Therefore, it is necessary to provide a new technical solution to overcome the above-mentioned defects. Summary of the invention

[0006] The purpose of the present invention is to provide a preparation method of a water shield extract and its application in cosmetics that can effectively solve the above technical problems; the method can not only fully extract the material components in the water shield, but also add the extracted water shield extract to cosmetics with the effect of repairing sunburn, which can significantly alleviate and accelerate the recovery of skin after sunburn.

[0007] In order to achieve the purpose of the present invention, the following technical scheme is adopted:

[0008] A preparation method of a Brasenia schrenkiana extract, wherein the preparation method of the Brasenia schrenkiana extract is as follows:

[0009] Step S1: clean and crush fresh water shield, then add water and complex enzyme to perform enzymolysis to obtain an enzymolysis product;

[0010] Step S2: adding a fermentation agent to the enzymatic hydrolysis product for fermentation to obtain a fermentation product;

[0011] Step S3: subjecting the fermentation product to ultrasonic treatment, and then taking it out and grinding it in a grinder to obtain a slurry;

[0012] And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid in a vacuum concentration evaporator for vacuum concentration to obtain the Brasenia schrenkiana extract.

[0013] Preferably, in step S1, the complex enzyme is composed of cellulase, xylanase, pectinase and amylase, and the mass ratio of cellulase, xylanase, pectinase and amylase is 1:0.3:0.1:0.2.

[0014] Preferably, the mass ratio of the water shield to the complex enzyme is 100:(1-5).

[0015] Preferably, in step S1, the enzymolysis temperature is 45-60°C and the enzymolysis time is 3-5h.

[0016] Preferably, in step S2, the fermentation bacteria agent is a composite of Bacillus subtilis, Lactobacillus plantarum and Propionibacterium freudenreichii, and the addition ratio of the fermentation bacteria liquid is 3-7% of the weight of the enzymatic hydrolysis product.

[0017] Preferably, the inoculation amount of Bacillus subtilis is (1×10 6 -3×10 8 )CFU / m;

[0018] The inoculation amount of the plant lactobacillus is (3×10 6 -10×10 8 )CFU / m;

[0019] The inoculum size of Propionibacterium freudenreichii is (2×10 6 -7×10 8 )CFU / m.

[0020] Preferably, the temperature of the ultrasonic treatment process in step S3 is 30-35° C. and the duration is 1-1.5 h.

[0021] Preferably, the vacuum concentration in step S4 is carried out under the condition of 100 kPa, and the concentration time is 40-50 min, until the soluble solids are 35-40%.

[0022] Furthermore, the present invention also provides a Brasenia schrenkiana extract prepared by the method described above; and provides the use of the Brasenia schrenkiana extract in cosmetics, specifically, the Brasenia schrenkiana extract is added into cosmetics with sunburn repairing effect at a ratio of 10-50%.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The method adopted by the present invention can not only more fully extract the effective components in the water shield by first enzymolyzing the water shield and then fermenting, ultrasonically treating and grinding the water shield, but also the prepared water shield extract has good sunburn repairing effect. Adding the water shield extract to cosmetics with sunburn repairing effect can significantly alleviate and accelerate the recovery of skin after sun exposure. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are partial embodiments of the present invention, rather than all embodiments.

[0026] The present invention provides a preparation method of a Brasenia schrenkiana extract, and the preparation method of the Brasenia schrenkiana extract is as follows:

[0027] Step S1: clean and crush fresh water shield, then add water and composite enzyme for enzymolysis to obtain enzymolysis product; wherein the composite enzyme is composed of cellulase, xylanase, pectinase and amylase, and the mass ratio of cellulase, xylanase, pectinase and amylase is 1:0.3:0.1:0.2; the mass ratio of water shield to composite enzyme is 100:(1-5); the enzymolysis temperature is 45-60°C, and the enzymolysis time is 3-5h.

[0028] Step S2: adding a fermentation agent to the enzymolysis product for fermentation treatment to obtain a fermentation product; wherein the fermentation agent is a composite of Bacillus subtilis, Lactobacillus plantarum and Propionibacterium freudenreichii, and the addition ratio of the fermentation solution is 3-7% of the weight of the enzymolysis product; the inoculation amount of Bacillus subtilis is (1×10 6 -3×10 8 ) CFU / m; the inoculum amount of the plant lactobacillus is (3×10 6 -10×10 8 ) CFU / m; the inoculum size of the Propionibacterium freudenreichii is (2×10 6 -7×108 )CFU / m.

[0029] Step S3: subjecting the fermentation product to ultrasonic treatment, and then taking it out and grinding it in a grinder to obtain a slurry; wherein the temperature of the ultrasonic treatment process is 30-35° C., and the duration is 1-1.5 h.

[0030] And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid into a vacuum concentration evaporator for vacuum concentration to obtain a water shield extract, wherein the vacuum concentration is carried out under the condition of 100 kPa, the concentration time is 40-50 minutes, and the soluble solid matter is 35-40%.

[0031] In addition, the present invention also provides a Brasenia schrenkiana extract prepared by the method described above; and provides the use of the Brasenia schrenkiana extract in cosmetics, specifically, the Brasenia schrenkiana extract is added into cosmetics with sunburn repairing effect at a ratio of 10-50%.

[0032] Example 1

[0033] The steps for preparing Brasenia schrenkiana extract-1 are as follows:

[0034] A preparation method of a Brasenia schrenkiana extract, wherein the preparation method of the Brasenia schrenkiana extract is as follows:

[0035] Step S1: 100 g of fresh water shield was washed and crushed, and then 150 g of water and 1 g of complex enzyme (the mass ratio of cellulase, xylanase, pectinase and amylase was 1:0.3:0.1:0.2) were added, and enzymolysis was performed at 50° C. for 4 h to obtain an enzymolysis product;

[0036] Step S2: adding a fermentation agent of 3% of the weight of the enzymatic hydrolysis product to the enzymatic hydrolysis product, and fermenting at 30° C. for 24 hours to obtain a fermentation product;

[0037] Step S3: subjecting the fermentation product to ultrasonic treatment at 30-35° C. for 1-1.5 h, and then taking it out and grinding it in a grinder to obtain a slurry.

[0038] And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid into a vacuum concentration evaporator, and concentrating it at 100 kPa for 40-50 minutes until the soluble solid matter is 35-40%, thereby obtaining the Brasenia schrenkiana extract.

[0039] Example 2

[0040] The steps for preparing Brasenia schrenkiana extract-2 are as follows:

[0041] A preparation method of a Brasenia schrenkiana extract, wherein the preparation method of the Brasenia schrenkiana extract is as follows:

[0042] Step S1: 100 g of fresh water shield was washed and crushed, and then 150 g of water and 2 g of complex enzyme (the mass ratio of cellulase, xylanase, pectinase and amylase was 1:0.3:0.1:0.2) were added, and enzymolysis was performed at 50° C. for 4 h to obtain an enzymolysis product;

[0043] Step S2: adding a fermentation agent of 4% of the weight of the enzymolysis product to the enzymolysis product, and fermenting at 30° C. for 24 hours to obtain a fermentation product;

[0044] Step S3: subjecting the fermentation product to ultrasonic treatment at 30-35° C. for 1-1.5 h, and then taking it out and grinding it in a grinder to obtain a slurry.

[0045] And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid into a vacuum concentration evaporator, and concentrating it at 100 kPa for 40-50 minutes until the soluble solid matter is 35-40%, thereby obtaining the Brasenia schrenkiana extract.

[0046] Example 3

[0047] The steps for preparing Brasenia schrenkiana extract-3 are as follows:

[0048] A preparation method of a Brasenia schrenkiana extract, wherein the preparation method of the Brasenia schrenkiana extract is as follows:

[0049] Step S1: 100 g of fresh water shield was washed and crushed, and then 150 g of water and 3 g of complex enzyme (the mass ratio of cellulase, xylanase, pectinase and amylase was 1:0.3:0.1:0.2) were added, and enzymolysis was performed at 50° C. for 4 h to obtain an enzymolysis product;

[0050] Step S2: adding a fermentation agent of 5% by weight of the enzymolysis product to the enzymolysis product, and fermenting at 30° C. for 24 hours to obtain a fermentation product;

[0051] Step S3: subjecting the fermentation product to ultrasonic treatment at 30-35° C. for 1-1.5 h, and then taking it out and grinding it in a grinder to obtain a slurry.

[0052] And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid into a vacuum concentration evaporator, and concentrating it at 100 kPa for 40-50 minutes until the soluble solid matter is 35-40%, thereby obtaining the Brasenia schrenkiana extract.

[0053] Example 4

[0054] The steps for preparing Brasenia schrenkiana extract-4 are as follows:

[0055] A preparation method of a Brasenia schrenkiana extract, wherein the preparation method of the Brasenia schrenkiana extract is as follows:

[0056] Step S1: 100 g of fresh water shield was washed and crushed, and then 150 g of water and 4 g of complex enzyme (the mass ratio of cellulase, xylanase, pectinase and amylase was 1:0.3:0.1:0.2) were added, and enzymolysis was performed at 50° C. for 4 h to obtain an enzymolysis product;

[0057] Step S2: adding 3-5% of the weight of the enzymatic hydrolysate into a fermentation agent, and fermenting at 30° C. for 24 hours to obtain a fermentation product;

[0058] Step S3: subjecting the fermentation product to ultrasonic treatment at 30-35° C. for 1-1.5 h, and then taking it out and grinding it in a grinder to obtain a slurry.

[0059] And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid into a vacuum concentration evaporator, and concentrating it at 100 kPa for 40-50 minutes until the soluble solid matter is 35-40%, thereby obtaining the Brasenia schrenkiana extract.

[0060] Example 5

[0061] The steps for preparing Brasenia schrenkiana extract-5 are as follows:

[0062] A preparation method of a Brasenia schrenkiana extract, wherein the preparation method of the Brasenia schrenkiana extract is as follows:

[0063] Step S1: 100 g of fresh water shield was washed and crushed, and then 150 g of water and 5 g of complex enzyme (the mass ratio of cellulase, xylanase, pectinase and amylase was 1:0.3:0.1:0.2) were added, and enzymolysis was performed at 50° C. for 4 h to obtain an enzymolysis product;

[0064] Step S2: adding a fermentation agent of 7% of the weight of the enzymolysis product to the enzymolysis product, and fermenting at 30° C. for 24 hours to obtain a fermentation product;

[0065] Step S3: subjecting the fermentation product to ultrasonic treatment at 30-35° C. for 1-1.5 h, and then taking it out and grinding it in a grinder to obtain a slurry.

[0066] And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid into a vacuum concentration evaporator, and concentrating it at 100 kPa for 40-50 minutes until the soluble solid matter is 35-40%, thereby obtaining the Brasenia schrenkiana extract.

[0067] Comparative Example

[0068] The steps of preparing Brasenia schrenkiana extract-6 by conventional water extraction are as follows:

[0069] 1) Rinse the water shield with cold water and soak it for 8-10 hours, then soak it in 60-70°C water for 2-4 hours, then crush it and soak it in ethanol for 6-8 hours to obtain a crude extract;

[0070] 2) Centrifuging the crude extract in step 1) and collecting the filtrate, and then removing ethanol from the filtrate to obtain the product.

[0071] The water shield extracts of Examples 1-5 and the comparative example are incorporated into cosmetics having sunburn repairing effects, including but not limited to emulsions, creams, gels, and the like.

[0072] Taking the sunburn repair gel as an example, the water shield extracts of Examples 1-5 and the comparative example were added to the sunburn repair gel to prepare after-sun repair gels 1-7, and the sunburn repair effects of the sunburn repair gels were tested.

[0073] The formula of the sunburn repair gel is shown in Table 1 below, and the amount of each component added is calculated by weight percentage.

[0074] Table 1 Sunburn repair gel formula

[0075]

[0076]

[0077] Effect test

[0078] 1. Test samples: After-sun repair gel 1-7 prepared according to the formula in Table 1.

[0079] 2. Test method: 210 volunteers with healthy skin, no history of skin allergies and sunburn symptoms were selected to participate in the test, including 130 females and 80 males. They were randomly divided into 7 groups according to gender, age, etc., with 30 people in each group. They tried gels 1-7 respectively. During the trial period, they washed the sunburned area with water in the morning, noon and evening, and then applied an appropriate amount of after-sun repair gel to the sunburned area. The trial lasted for 2 weeks. After the trial, the volunteers evaluated the effects of the after-sun repair gel, including hydrating and moisturizing effect, rapid analgesic and antipruritic effect (i.e., swelling, pain and itching symptoms were significantly reduced within 5 to 20 minutes), rapid erythema fading effect (diffuse erythema was significantly subsided or disappeared within 3 hours), anti-pigmentation effect (i.e., tanning was not obvious or only slightly tanned), refreshing and non-greasy feeling, and rapid repair effect of second-degree sunburn (i.e., peeling symptoms were significantly improved or disappeared within 4 days, and blisters began to scab and fall off). They gave scores between 0 and 10 points according to the effects, with 9 to 10 points being very good, 7 to 8 points being good, 5 to 6 points being average, 3 to 4 points being poor, and 0 to 2 points being very poor, and the average value was taken.

[0080] 3. Experimental results: see Table 2.

[0081]

[0082] It can be seen from the contents of Table 2 that after adding the water shield extract prepared by the method of the present invention to the after-sun repair gel, the after-sun repair effect can be significantly improved, while the water shield extract prepared by the conventional water extraction method has no obvious promoting effect on after-sun repair.

[0083] Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention.

Claims

1. A method for preparing a Brasenia schrenkiana extract, characterized in that: The preparation method of the water shield extract is as follows: Step S1: clean and crush fresh water shield, then add water and complex enzyme to perform enzymolysis to obtain an enzymolysis product; Step S2: adding a fermentation agent to the enzymatic hydrolysis product for fermentation to obtain a fermentation product; Step S3: subjecting the fermentation product to ultrasonic treatment, and then taking it out and grinding it in a grinder to obtain a slurry; And, step S4: filtering the slurry to remove solid impurities in the slurry, and then placing the remaining liquid into a vacuum concentration evaporator for vacuum concentration to obtain a Brasenia schrenkiana extract; In the step S1, the complex enzyme is composed of cellulase, xylanase, pectinase and amylase, and the mass ratio of cellulase, xylanase, pectinase and amylase is 1:0.3:0.1:0.2; The mass ratio of the water shield to the complex enzyme is 100:(1-5); In step S1, the enzymatic hydrolysis temperature is 45-60°C and the enzymatic hydrolysis time is 3-5h; In the step S2, the fermentation bacteria agent is a composite of Bacillus subtilis, Lactobacillus plantarum and Propionibacterium freudenreichii, and the addition ratio of the fermentation bacteria liquid is 3-7% of the weight of the enzymatic hydrolysis product; The inoculum size of Bacillus subtilis is (1×10 6 -3×10 8 )CFU / m; The inoculation amount of the plant lactobacillus is (3×10 6 -10×10 8 )CFU / m; The inoculum size of Propionibacterium freudenreichii is (2×10 6 -7×10 8 )CFU / m; The temperature of the ultrasonic treatment process in step S3 is 30-35° C. and the duration is 1-1.5 h; In step S4, vacuum concentration is performed under the condition of 100 kPa, and the concentration time is 40-50 min, until the soluble solids are 35-40%.

2. A Brasenia schrenkiana extract prepared by the method according to claim 1.

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