Use of mangonia longipes extract in preparation of antioxidant medicine

Abstract

The application discloses application of a long-stalk mangrove extract in preparation of antioxidant medicines. The long-stalk mangrove extract is prepared by the following preparation method: long-stalk mangrove stems or leaves are immersed with ethanol, the immersion liquid is concentrated, then is dispersed with water, and then is extracted with petroleum ether, ethyl acetate and n-butanol in sequence, and the extract is concentrated to obtain petroleum ether parts, ethyl acetate parts and n-butanol parts respectively. The application firstly carries out research on natural active ingredients of the long-stalk mangrove, adopts petroleum ether, ethyl acetate, n-butanol and water as solvents, carries out distribution extraction on stems and leaves of the long-stalk mangrove, and then evaluates antioxidant activities of the extracted parts, so as to find effective parts and pharmacological activities of the long-stalk mangrove. The research result can not only provide a reference for extraction of natural plant effective ingredients by different extractants, but also provide important guiding significance for comprehensive development of the long-stalk mangrove resources.

Description

Technical Field

[0001] This invention belongs to the field of biomedicine, specifically relating to the application of extracts from *Magnolia denudata* in the preparation of antioxidant drugs. Background technology:

[0002] *Manglietia longipedunculata* Zeng et Law is an evergreen broad-leaved plant belonging to the genus *Manglietia* in the family Magnoliaceae. It typically grows in forests at altitudes of 650-800 meters. With excellent timber quality, it is an outstanding ornamental and timber species with high economic value. *Manglietia longipedunculata* was first discovered in 2003 by a research team from the South China Botanical Garden (South China National Botanical Garden) of the Chinese Academy of Sciences during a field survey in the Nankun Mountains. Due to a lack of pollinating insects, asynchronous development of stamens and pistils, and an extremely short receptive period of the stigma, this species is very difficult to reproduce, and in its natural state, it essentially only flowers and does not bear fruit. Only one population of *Manglietia longipedunculata* has been found worldwide in the Nankun Mountains Provincial Nature Reserve; its population size is extremely small, classifying it as a rare and endangered plant.

[0003] Currently, research on Magnolia longipes mainly focuses on its artificial propagation and introduction cultivation, while research on the efficacy and utilization of Magnolia longipes has not yet been reported. Summary of the Invention:

[0004] The purpose of this invention is to provide an application of *Magnolia denudata* extract in the preparation of antioxidant drugs.

[0005] This invention provides the application of *Magnolia longipes* extract in the preparation of antioxidant drugs. The *Magnolia longipes* extract is prepared by the following method: extracting *Magnolia longipes* stems or leaves with ethanol, concentrating the extract and dispersing it with water, then extracting it sequentially with petroleum ether, ethyl acetate and n-butanol, and concentrating the extract to obtain the petroleum ether fraction, ethyl acetate fraction and n-butanol fraction respectively.

[0006] Preferably, the extract of Magnolia longipes is the petroleum ether fraction or the ethyl acetate fraction of the stem or leaves of Magnolia longipes.

[0007] Preferably, the concentration is vacuum concentration under reduced pressure, and the temperature does not exceed 60°C.

[0008] The present invention also provides an antioxidant drug containing *Magnolia longipes* extract as an active ingredient. The *Magnolia longipes* extract is prepared by the following method: extracting *Magnolia longipes* stems or leaves with ethanol, concentrating the extract and dispersing it with water, and then extracting it sequentially with petroleum ether, ethyl acetate and n-butanol. The extracts are then concentrated to obtain the petroleum ether fraction, ethyl acetate fraction and n-butanol fraction, respectively.

[0009] Preferably, the extract of Magnolia longipes is the petroleum ether fraction or the ethyl acetate fraction of the stem or leaves of Magnolia longipes.

[0010] This invention is the first to study the natural active ingredients of *Magnolia longipes*. Petroleum ether, ethyl acetate, n-butanol, and water were used as solvents for the distribution extraction of the stems and leaves of *Magnolia longipes*. The antitumor, anti-inflammatory, and antioxidant activities of each extracted fraction were then evaluated to identify the effective components and pharmacological activities of *Magnolia longipes*. The research results not only provide a reference for the extraction of effective components from natural plants using different extractants but also offer important guidance for the comprehensive development of *Magnolia longipes* resources. Attached Figure Description

[0011] Figure 1 It is the antioxidant activity of different extracts from Magnolia longipes. Detailed implementation method:

[0012] The following embodiments are further illustrations of the present invention, but not limitations thereof.

[0013] Example 1:

[0014] Experimental Procedures and Results

[0015] 1. Preparation of crude extracts from stems and leaves of *Magnolia longipes* using different solvents

[0016] 1.1 Preparation of fresh green leaves of Magnolia longipes

[0017] First, 100g of fresh green leaves from *Magnolia longipes* were soaked in 300mL of anhydrous ethanol for 24 hours, followed by concentration of the extract. This soaking process was repeated three times. The three ethanol extracts were then combined and concentrated under vacuum (temperature not exceeding 60℃). The concentrated extract was dispersed in water, first extracted three times with petroleum ether to obtain the petroleum ether extract, then three times with ethyl acetate to obtain the ethyl acetate extract, and finally extracted with n-butanol to obtain the n-butanol extract. The remaining fraction was the aqueous fraction. Each fraction was then concentrated under vacuum (temperature not exceeding 60℃).

[0018] 1.2 Preparation of crude extracts from fresh stems and branches of Magnolia longipes

[0019] First, 100g of fresh stems and branches of *Magnolia longipes* were soaked in 300mL of anhydrous ethanol for 24 hours, followed by concentration of the extract. This soaking process was repeated three times. The three ethanol extracts were then combined and concentrated under vacuum (temperature not exceeding 60℃). The concentrated extract was dispersed in water, first extracted three times with petroleum ether to obtain the petroleum ether extract, then three times with ethyl acetate to obtain the ethyl acetate extract, and finally extracted with n-butanol to obtain the n-butanol extract. The remaining fraction was the aqueous fraction. Each fraction was then concentrated under vacuum (temperature not exceeding 60℃).

[0020] 1.3 Antioxidant Activity Test

[0021] RAW264.7 cells were grown at a rate of 4 × 10⁻⁶. 4 Seeds were planted at a density of 10 ...

[0022] The results of antioxidant activity determination of different extracts of Magnolia longipes are shown in Table 1 and Figure 1 As shown in the figure. Experimental results indicate that extracts of *Magnolia longipes* also possess good antioxidant activity. Among the four stem extracts, ethyl acetate and n-butanol fractions exhibited the best antioxidant activity, with inhibition rates of 88.24% and 88.78% for intracellular reactive oxygen species (ROS), respectively. Petroleum ether and aqueous fractions showed weak antioxidant activity, with inhibition rates of 60.44% and 56.31%, respectively. Among the four leaf extracts, ethyl acetate fraction showed the best antioxidant activity, with an inhibition rate of 93.93% for ROS, followed by n-butanol fraction with an inhibition rate of 75.02%. The petroleum ether and aqueous fractions of the leaves showed weak antioxidant activity, with inhibition rates of 64.98% and 48.51% for ROS at a concentration of 1 mg / mL, respectively. These experimental results indicate that extracts of *Magnolia longipes* possess strong antioxidant activity, mainly concentrated in the ethyl acetate and n-butanol fractions of the stems and the ethyl acetate fraction of the leaves. This suggests that future research could focus on these two parts, potentially leading to the discovery of antioxidant lead compounds for the development of new functional foods and antioxidant drugs.

[0023] Table 1. Antioxidant activity (inhibition rate %) of different solvent extracts from the stems and leaves of Magnolia longipes.

[0024]

[0025] The antioxidant activity IC50 of different solvent extracts of Magnolia longipes was determined using a cell model. 50 The results are shown in Table 2. Among the four extracts of the stem, the antioxidant activity ranked as follows: n-butanol fraction > ethyl acetate fraction > petroleum ether fraction > aqueous fraction; similarly, among the four extracts of the leaf fraction, the antioxidant activity ranked as follows: ethyl acetate fraction > n-butanol fraction > petroleum ether fraction > aqueous fraction. This indicates that the n-butanol fraction of the stem and the ethyl acetate fraction of the leaf have promising development and application prospects.

[0026] Table 2. Antioxidant activity (IC50) of different solvent extracts from the stems and leaves of Magnolia longipes. 50 (μg / mL)

[0027]

[0028]

Claims

1. Application of *Magnolia longipes* extract in the preparation of antioxidant drugs, wherein the *Magnolia longipes* extract is prepared by the following method: extracting *Magnolia longipes* stems or leaves with ethanol, concentrating the extract and dispersing it with water, and then extracting it sequentially with petroleum ether, ethyl acetate and n-butanol, and concentrating the extract to obtain the petroleum ether fraction, ethyl acetate fraction and n-butanol fraction respectively, wherein the *Magnolia longipes* extract is the ethyl acetate fraction and n-butanol fraction of *Magnolia longipes* stems or leaves.

2. The application according to claim 1, characterized in that, The concentration described is vacuum concentration under reduced pressure, with the temperature not exceeding 60°C. o C.

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