Binary insecticide composition and use thereof

Abstract

The present invention discloses an insecticide synergistic composition containing a piperic acid derivative compound and an application thereof. The composition consists of two active ingredients, compound A and compound B, wherein the active component A is compound I-72; the active component B is selected from any one of indoxacarb, s-indoxacarb or metaflumizone; the compound I-72 has the following structure: The composition of the present invention has the advantages of obvious synergy, delayed resistance, etc., and can be used for preventing and controlling lepidopteran and coleopteran pests on agriculture, forestry and ornamental plants, especially for use on resistant striped stem borer and resistant diamondback moth.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide application, and particularly relates to a binary insecticide composition containing a piperic acid derivative compound mixed with any one of indoxacarb, indoxacarb-s-carb or metaflumizone and a use thereof. Background Art

[0002] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, wherein compound I-72 has contact and stomach poison activity, good conduction activity, and can be used to control a variety of Lepidoptera, Hemiptera, Thysanoptera, Coleoptera and other pests. The compound has a novel structure, a broad insecticide spectrum, high activity, and no cross-resistance with other insecticides, and is expected to become an environmentally friendly agent for the control of resistant pests.

[0003] Indoxacarb is the first commercial diazinon insecticide developed by DuPont in the United States. It has contact and stomach poison effects and is effective against larvae of all ages. It can effectively control a variety of pests on crops such as grain, cotton, fruit and vegetables, such as beet armyworm, diamondback moth, cabbage worm, cutworm, cabbage armyworm, cotton bollworm, tobacco budworm, leaf roller, codling moth, leafhopper, golden guillotine, potato beetle, etc. on cabbage, cauliflower, kale, tomato, pepper, cucumber, courgette, eggplant, lettuce, apple, pear, peach, apricot, cotton, potato, grape and other crops. Research reports on the resistance mechanism of indoxacarb show that diamondback moth, cutworm and beet armyworm in different regions have developed different degrees of resistance to indoxacarb, and other insects have the risk of developing high levels of resistance to indoxacarb.

[0004] Metaflumizone is a semicarbazone insecticide jointly developed by BASF of Germany and Japan Agricultural Chemicals Co., Ltd. It has both stomach poison and contact killing effects. Its mechanism of action is similar to that of indoxacarb. Both are new sodium channel inhibitors with a broad insecticide spectrum, harmless to mammals and beneficial insects, and safe for the environment. It can effectively prevent and control various lepidopteran pests and the larvae and adults of some coleoptera.

[0005] In order to overcome the limitations of single-ingredient insecticides in pest control, such as narrow insecticide spectrum, high cost, poor rapid effect, and pests are very likely to develop resistance after continuous use. Two insecticides with different action mechanisms are mixed into mixed components in a certain proportion, so as to overcome the shortcomings of single drug use, improve drug efficacy, reduce dosage, and extend the service life of the variety. Summary of the invention

[0006] The present invention aims at a binary insecticide composition containing a piperic acid derivative compound mixed with any one of indoxacarb, fine indoxacarb or metaflumizone and its use.

[0007] To achieve the above purpose, the present invention adopts the following technical solution:

[0008] A binary insecticide composition, the composition is composed of two active ingredients A and B, wherein the active component A is selected from compound I-72, and the active component B is selected from indoxacarb, indoxacarb-sperm or metaflumizone; the weight ratio of the two active components in the composition is 100:1 to 1:50;

[0009] The active component A has the following structure:

[0010]

[0011] Preferably, the active component A is selected from compound I-72, and the active component B is selected from indoxacarb, indoxacarb-S or metaflumizone; the weight ratio of the two active components is 20:1 to 1:20.

[0012] Further preferably, the active component A is selected from compound I-72, and the active component B is selected from indoxacarb or indoxacarb-S; the weight ratio of the two active components is 10:1 to 1:10.

[0013] More preferably, the active component A is selected from compound I-72, and the active component B is selected from metaflumizone, and the weight ratio of the two active components in the composition is 10:1 to 1:10.

[0014] The invention discloses an application of a binary insecticide composition, wherein the composition is used for controlling lepidopteran and coleopteran pests on agricultural, forestry and ornamental plants, especially for controlling resistant chilo suppressalis and resistant diamondback moth.

[0015] The composition is applied to the pests to be controlled or the medium where they grow at an effective dose.

[0016] Indoor biological activity determination shows that the binary insecticide composition of the present invention has a synergistic effect. For example, compound I-72 and indoxacarb have a significant synergistic effect on beet armyworm in the range of 1:1 to 1:10, and have a significant synergistic effect on fall armyworm in the range of 10:1 to 1:10; compound I-72 and metaflumizone have a synergistic effect on corn borer in the range of 10:1 to 1:10, and have a synergistic effect on diamondback moth in the range of 10:1 to 1:10.

[0017] The composition of the invention is suitable for preventing and controlling various lepidoptera and coleoptera pests that harm agricultural and forestry crops and resistant striped stem borer, diamondback moth or beet armyworm.

[0018] The lepidopteran pests include, but are not limited to, the following species, such as the striped stem borer, rice leaf folder, yellow stem borer, stem borer, armyworm, cotton bollworm, beet armyworm, armyworm, cabbage armyworm, gypsy moth, cutworm, looper, peach borer, pear borer, fall armyworm, diamondback moth, and cabbage worm.

[0019] The coleopteran pests include, but are not limited to, the following species: flea beetles, twenty-eight-spotted ladybirds, potato beetles, wireworms, and white grubs.

[0020] The composition of the present invention is applicable to but not limited to the following crops or plants:

[0021] Cereal crops, such as wheat, barley, rye, oats, rice, corn, sorghum, millet, etc.; tuber crops: including sweet potatoes, yams, potatoes, etc.; legume crops: including soybeans, broad beans, peas, mung beans, etc.; fiber crops: including cotton, jute, kenaf, hemp, flax, sisal, mat grass, etc.; oil crops: including oilseeds, iris, mustard, peanuts, sesame, hemp, sunflower, etc.; sugar crops: including sugarcane, sugar beets, etc.; Beverage crops: including tea, coffee, cocoa, etc.; dye crops: including indigo, saffron, etc.; spice crops: including lemongrass, mint, rose; feed crops: including alfalfa, sweet clover, astragalus, vetch, clover, etc.; feed crops include ryegrass, oat grass, Sudan grass, etc.; green manure crops, such as vetch, astragalus, yellow alfalfa, sweet clover, tamarind, sesbania, amorpha, etc.; fruits, such as apples, pears, plums, peaches, apricots kernel, cherry, citrus, mango, litchi, banana, grape; or berry, such as strawberry, raspberry or blackberry mustard, poppy, olive, coconut, castor, etc.; vegetable, such as cucumber, sponge gourd, watermelon, melon, pumpkin, hanging melon, spinach, celery, cabbage, cabbage, cauliflower, gourd, pepper, eggplant, tomato, onion, ginger, garlic, leek, lettuce, kidney bean, cowpea, radish, carrot, yam, etc.; the composition of the present invention can also be used for Any ornamental plant, including flowers, shrubs, broadleaf trees and evergreens, for example, pansies, begonias, dahlias, gerberas, hydrangeas, verbenas, roses, galanthus, poinsettias, aster, cornflowers, coreopsis, delphiniums, monopetalums, phlox, rhodiola, sedums, petunias, violets, impatiens, geraniums, chrysanthemums, ranunculus, sage, valerian, rosemary, sage, St. John’s wort, mint.

[0022] Compared with the prior art, the composition of the present invention has the following advantages:

[0023] First, the composition of the present invention exhibits an obvious synergistic effect within a certain ratio range, thereby improving the control effect of the compound on pests.

[0024] Secondly, the active component A selected for the composition has the characteristics of a broad insecticidal spectrum and high insecticidal activity. Its successful application in the binary insecticidal composition broadens the insecticidal spectrum and can achieve the purpose of controlling multiple pests with one application, thereby reducing the cost of control.

[0025] Third, the effective ingredients in the composition have different mechanisms of action, and there is no problem of cross-resistance, so it can be used for the comprehensive control of resistant pests. DETAILED DESCRIPTION

[0026] The following specific examples are used to further illustrate the present invention in detail, but the present invention is by no means limited to these examples.

[0027] The synergistic binary insecticide composition containing compound I-72 of the present invention is used to effectively prevent and control lepidopteran and coleopteran pests on lepidopteran forestry and ornamental plants, especially on resistant striped stem borer and resistant diamondback moth.

[0028] The active component A in the embodiment is compound I-72 prepared according to the description in patent document CN112457288 A, with a content of 97%; the active component B and other raw materials are all commercially available products. The percentage or ratio of each component is measured by weight. The active component in each formula is calculated by effective content.

[0029] The structural formula of compound I-72 is as follows:

[0030] Indoor biological activity measurement example

[0031] The following examples are used to illustrate the technical solutions of the present invention, but the present invention is not limited to the following examples.

[0032] Example 1 Indoor joint toxicity test of the composition containing compound I-72 against Spodoptera exigua

[0033] Test subjects: Beet armyworm (Spodoptera exigua Hübner), 3rd instar larvae, sensitive strain reared indoors.

[0034] Test conditions: temperature: 24-26°C, humidity: RH 60%, light: L:D=14:10.

[0035] Preparation method: Use an electronic analytical balance to accurately weigh the test drugs, add appropriate amount of solvent to completely dissolve the two original drugs, prepare the mother solution of required concentration with water containing 0.1% Tween 80, mix the two active ingredient mother solutions in a certain proportion to prepare a mixed solution, and dilute them into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0036] Test method: Dipping method is adopted. Take cabbage leaves grown in the greenhouse, remove the waxy layer on the surface, use a hole puncher to make a leaf disc with a diameter of 3 cm, immerse it in the prepared solution for 10 seconds, put it in a culture dish with a diameter of 9 cm and lined with filter paper, dry it naturally in the shade, and inoculate with standard test insects, 10 insects per dish, and repeat 3 times. Place the treated test insects in an observation room with certain conditions, observe the reaction of the test insects regularly, and investigate the number of dead and alive insects after 72 hours.

[0037] Evaluation method: Sun YP method was used to calculate the co-toxicity coefficient of each mixture ratio and evaluate the type of combined action of each mixture.

[0038] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0039]

[0040] Where: ATI-actual toxicity index of the mixture; S-LC of the standard agent 50 ; LC of M-mixture 50 .

[0041] TTI=TIA×PA+TIB×PB

[0042] Where: TTI-theoretical toxicity index of mixture; TIA-toxicity index of A agent; percentage of PA-A agent in the mixture; TIB-toxicity index of B agent; percentage of PB-B agent in the mixture.

[0043]

[0044] Where: CTC-co-toxicity coefficient; ATI-actual toxicity index of mixture; TTI-theoretical toxicity index of mixture.

[0045] CTC - Co-toxicity coefficient

[0046] ATI——Actual Toxicity Index of Mixtures

[0047] TTI - Theoretical Toxicity Index

[0048] In the formula, the co-toxicity coefficient (CTC) of the mixture ≥ 120 indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; 80 < CTC < 120 indicates an additive effect.

[0049] The test results in Table 1 show that the mixture of compound I-72 and indoxacarb in the ratio range of 10:1 to 1:10 has a significant synergistic effect on beet armyworm.

[0050] Table 1 Indoor combined toxicity test of compound I-72 and indoxacarb in different ratios against Spodoptera exigua

[0051]

[0052] Example 2: Determination of the combined toxicity of different ratios of compound I-72 and metaflumizone against Ostrinia furnacalis

[0053] Test subjects: Corn borer (Pyrausta nubilalis Hubern), 3rd instar larvae, sensitive strain reared indoors.

[0054] Test method: artificial feed film method is used. Select a standard clean 24-well culture plate, add 1ml of artificial feed to each well, cool and solidify, add a certain amount of liquid medicine to the culture plate with a continuous sampler, and dry it naturally in the shade. Inoculate standard test insects, 1 head per well, a total of 48 heads. Place the treated test insects in an observation room with certain conditions, observe the test insects' reactions regularly, and investigate the number of dead and alive insects after 72 hours.

[0055] The test conditions, the preparation method of the drug solution and the evaluation method of the combined action type of the mixture (Sun YP) are the same as those in Example 1.

[0056] Compound I-72 and metaflumizone have obvious synergistic effects against corn borer in the ratio range of 10:1 to 1:10.

[0057] Table 2. Indoor combined toxicity test of different ratios of compound Ⅰ-72 and metaflumizone against corn borer

[0058]

[0059] Example 3: Indoor joint toxicity test of different ratios of the combination of compound I-72 and indoxacarb against fall armyworm

[0060] Test subjects: Fall armyworm (Spodoptera frugiperda), 3rd instar larvae, sensitive strain reared indoors

[0061] Test method: Dipping method was used. Take corn leaves grown in the greenhouse, cut them into 5cm long leaf segments, soak them in the prepared solution for 10s, dry them naturally in the shade, cut them into 1.5cm*1.5cm small pieces, apply them to a 24-well culture plate, and inoculate standard test insects, 48 ​​per treatment. Place the treated test insects in an observation room under certain conditions, observe the test insects' reactions regularly, and investigate the number of dead and alive insects after 72h.

[0062] The test conditions, the preparation method of the drug solution and the evaluation method of the combined action type of the mixture (Sun YP) are the same as those in Example 1.

[0063] As can be seen from the table (Table 3), compound I-72 and indoxacarb showed obvious synergistic effects on the third instar larvae of Spodoptera frugiperda at a ratio of 10:1 to 1:10.

[0064] Table 3 Results of indoor combined toxicity test of compound A and indoxacarb against fall armyworm

[0065]

[0066]

[0067] Example 4: Determination of the combined toxicity of different ratios of compound I-72 and chlorflumizone against Plutella xylostella

[0068] Test target: Diamondback moth (Plutella xylostella), 3rd instar larvae, susceptible strain reared indoors.

[0069] Test conditions: Temperature: 25-27°C, Humidity: RH 60%, Light: L:D=14:10

[0070] Test method: The airbrush spray method was used. Cabbage grown in the greenhouse was taken and made into leaf discs with a diameter of 3 cm. After being evenly sprayed with an airbrush manual sprayer, the discs were placed in a 9 cm culture dish lined with filter paper. After being naturally dried in the shade, standard test insects were inoculated and the number of dead and alive insects was investigated for 72 hours.

[0071] The preparation of the drug and the evaluation method of the combined action type of the mixture (Sun YP) are the same as those in Example 1.

[0072] Table 4 Results of indoor combined toxicity test of compound I-72 and metaflumizone against Plutella xylostella

[0073]

Claims

1. A binary insecticide composition, characterized in that: The composition consists of two active ingredients A and B, wherein the active component A is selected from compound I-72, and the active component B is selected from indoxacarb or metaflumizone; the weight ratio of the two active components in the composition is 100:1-1:50; The active component A has the following structure: Compound 1-72.

2. The binary insecticide composition according to claim 1, characterized in that: The active component A is selected from compound I-72, and the active component B is selected from indoxacarb or metaflumizone; the weight ratio of the two active components is 20:1 to 1:

20.

3. The composition according to claim 2, characterized in that: The active component A is selected from compound I-72, and the active component B is selected from indoxacarb; the weight ratio of the two active components is 10:1 to 1:

10.

4. The composition according to claim 2, characterized in that: The active component A is selected from compound I-72, and the active component B is selected from metaflumizone. The weight ratio of the two active components in the composition is 10:1 to 1:

10.

5. Use of the binary insecticide composition according to claims 1-4, characterized in that: The composition is used for controlling lepidoptera and coleoptera pests on agriculture, forestry and ornamental plants.

6. Use of the binary insecticide composition according to claim 5, characterized in that: The composition is used for controlling resistant Chilo suppressalis and resistant Diamondback moth on agricultural, forestry and ornamental plants.

7. Use of the binary insecticide composition according to claim 5, characterized in that: The composition is applied to the pests to be controlled or the medium where they grow at an effective dose.

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