A kind of cornus officinalis extract and its preparation method and application

Abstract

The present invention relates to a cornus officinalis extract and a preparation method and application thereof. The preparation method of the cornus officinalis extract comprises the following steps: (1) extracting cornus officinalis by mixing with an extraction solvent, centrifuging and filtering to obtain a sample solution; (2) concentrating and drying the sample solution to obtain the cornus officinalis extract. The present invention adopts a specific optimization process to extract cornus officinalis. The cornus officinalis extract prepared by the method of the present invention contains a high content of ursolic acid and other active ingredients, has a good antioxidant and anti-photoaging effect, can effectively prevent skin roughness and aging caused by ultraviolet rays in sunlight, keep the skin bright and bright, and rejuvenate the skin.

Description

Technical Field

[0001] The invention belongs to the technical field of plant natural products, and particularly relates to a cornus officinalis extract and a preparation method and application thereof. Background Art

[0002] Cornus officinalis is an economic shrub native to my country. It is distributed in Henan, Shandong, Shaanxi and other places. Its dried and mature fruits are commonly used in traditional Chinese medicine. It is also a plant with both medicinal and edible properties. Cornus officinalis fruit contains a variety of active ingredients, mainly including cyclopentadiene ether terpenes, polyphenols, triterpenes, polysaccharide organic acids, etc. Among them, the triterpenes are mainly ursolic acid and oleanolic acid. Ursolic acid, also known as ursolic acid, is a weakly polar pentacyclic triterpene acid. It is widely distributed in nature and is a functional component of many natural products. It is soluble in methanol and ethanol, but insoluble in water and petroleum ether. Modern medical research shows that ursolic acid has a variety of biological effects such as sedation and anti-inflammatory, and also has a significant antioxidant function, so it is widely used as a raw material for medicine and cosmetics. Excessive exposure to ultraviolet radiation can have a negative impact on human skin, which is characterized by photodamage. Traditional Chinese medicine has its unique advantages in preventing and treating skin photoaging, including phenolic acids, flavonoids, polysaccharides, triterpenes, etc. Studies have shown that ursolic acid in triterpenes has anti-photoaging effects. Cornus officinalis contains a large amount of ursolic acid. Currently, research on extracting the bioactive compound ursolic acid from Cornus officinalis to resist photoaging is very limited.

[0003] At present, Cornus officinalis extract has been used in cosmetics. Chinese patent CN201580017172.2 discloses an anti-wrinkle composition and an anti-wrinkle cream made from Cornus officinalis extract. The product can easily penetrate the skin, significantly relax excessive and single muscle tension, and relieve and improve existing wrinkles.

[0004] Chinese patent CN201410644298.X discloses a composition of cornus officinalis extract for skin wrinkle prevention, which contains ursolic acid extracted from cornus officinalis, has excellent skin safety, and has the effect of preventing skin aging and improving skin wrinkles. Chinese patent CN201310052487.3 discloses a whitening cosmetic containing cornus officinalis ethanol extract, which can improve skin metabolism, promote skin blood circulation, increase skin nutrition, effectively remove free radicals, eliminate pigmentation, and reduce wrinkles and spots on the skin, making the skin more delicate, smooth, white and elastic. Chinese patent CN201811640348.1 discloses an anti-aging composition containing cornus officinalis extract, which has significant effects.

[0005] In general, there are cosmetics containing Cornus officinalis extracts on the market, which are mainly used for anti-aging, and there are few reports on their use in anti-photoaging. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a Cornus officinalis extract and a preparation method and application thereof, and in particular to provide a Cornus officinalis extract with anti-photoaging effect and a preparation method and application thereof.

[0007] To achieve this object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a method for preparing a Cornus officinalis extract, the method for preparing the Cornus officinalis extract comprising the following steps:

[0009] (1) extracting cornus officinalis by mixing with an extraction solvent, centrifuging and filtering to obtain a sample solution;

[0010] (2) The sample solution is concentrated and dried to obtain a Cornus officinalis extract.

[0011] The present invention adopts a specific optimization process to extract Cornus officinalis. The Cornus officinalis extract prepared by the method of the present invention contains a high level of active ingredients such as ursolic acid, has a good antioxidant and anti-photoaging effect, can effectively prevent skin roughness and aging caused by ultraviolet rays in sunlight, keep the skin bright and shiny, and rejuvenate the skin.

[0012] The invention utilizes the extraction method to extract ursolic acid from Cornus officinalis, which is time-consuming and simple in process. Meanwhile, the extraction solvent can be recycled and reused, and the economic benefit is good.

[0013] Preferably, the extraction solvent is selected from any one of ethanol, methanol, glycerol, ethylene glycol, acetone or acetic acid, or a combination of at least two thereof; preferably a combination of ethanol, glycerol and acetone.

[0014] Preferably, the volume ratio of ethanol, glycerol and acetone is (1-4):(3-6):(5-10).

[0015] The present invention adopts a specific combination of organic solvents, namely ethanol, glycerol and acetone, as an extraction solvent. When the combination of the organic solvents meets a specific volume ratio, the ursolic acid content in the extract is higher and the effects in terms of anti-oxidation and anti-photoaging are better.

[0016] Specific point values ​​of 1-4 include 1, 2, 3, 3.5, 4, etc.; specific point values ​​of 3-6 include 3, 4, 4.5, 5, 5.5, 6, etc.; specific point values ​​of 5-10 include 5, 6, 7, 8, 9, 10, etc.; specific point values ​​within the above numerical range can be selected and will not be listed here one by one.

[0017] Preferably, the mass volume ratio of Cornus officinalis and the extraction solvent is (2-6):(20-60) g / mL.

[0018] The point values ​​of 2-6 may be, for example, 2, 2.3, 2.5, 3, 3.3, 3.5, 4, 5, 5.5, 6, etc.; the specific point values ​​of 20-60 may be, for example, 20, 25, 30, 35, 40, 45, 50, 55, 60, etc.; any specific point values ​​within the above numerical range may be selected and will not be described one by one here.

[0019] Preferably, the extraction method is selected from any one of ultrasound-assisted extraction, enzyme-assisted extraction or microwave-assisted extraction, or a combination of at least two thereof; further preferred is a combination of ultrasound-assisted extraction and microwave-assisted extraction.

[0020] Preferably, the ultrasound-assisted extraction and microwave-assisted extraction are performed simultaneously.

[0021] Preferably, the extraction temperature is 10-50°C.

[0022] Preferably, the extraction time is independently 20-40 min.

[0023] Preferably, the power of the ultrasound-assisted extraction is 80W-120W.

[0024] Preferably, the power of the microwave-assisted extraction is 500-700W.

[0025] The specific point values ​​of 10-50 may be, for example, 10, 15, 20, 25, 30, 35, 40, 45, 50, etc.; the specific point values ​​of 20-40 may be, for example, 20, 23, 25, 30, 33, 35, 38, 40, etc.; the specific point values ​​of 80-120 may be 80, 85, 90, 95, 100, 105, 110, 115, 120, etc.; the specific point values ​​of 500-700 may be 500, 530, 580, 600, 650, 660, 680, 700, etc.; the specific point values ​​within the above numerical range may be selected, and will not be described one by one here.

[0026] Preferably, the concentration comprises any one of rotary evaporation, precipitation or recrystallization, or a combination of at least two thereof.

[0027] Preferably, the drying comprises any one of freeze drying, boiling drying or spray drying, or a combination of at least two thereof.

[0028] In a second aspect, the present invention provides a Cornus officinalis extract prepared by the preparation method described in the first aspect.

[0029] In a third aspect, the present invention provides use of the Cornus officinalis extract according to the second aspect in preparing an anti-skin photoaging product.

[0030] Preferably, the product comprises a skin care product.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention adopts a specific optimization process to extract Cornus officinalis. The Cornus officinalis extract prepared by the method of the present invention contains a high level of active ingredients such as ursolic acid, has a good antioxidant and anti-photoaging effect, can effectively prevent skin roughness and aging caused by ultraviolet rays in sunlight, keep the skin bright and shiny, and rejuvenate the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a graph showing the effect of Cornus officinalis extract on the life span of nematodes under UVB irradiation;

[0034] Figure 2 This is the result diagram of the effect of Cornus officinalis extract on the relative lifespan of nematodes under UVB irradiation;

[0035] Figure 3 This is the fluorescence image of the active oxygen in nematodes irradiated by UVB by the extract of Cornus officinalis.

[0036] Figure 4 This is the result diagram of the effect of Cornus officinalis extract on the level of active oxygen in nematodes after UVB irradiation;

[0037] Figure 5 This is the result diagram of the effect of Cornus officinalis extract on superoxide dismutase activity in nematodes after UVB irradiation;

[0038] Figure 6 This is the result diagram of the effect of Cornus officinalis extract on malondialdehyde (MDA) in nematodes after UVB irradiation;

[0039] Figure 7 This is a graph showing the effect of Cornus officinalis extract on the expression levels of related genes in nematodes after UVB irradiation. DETAILED DESCRIPTION

[0040] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and through specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0041] Example 1

[0042] This embodiment provides a cornus officinalis extract and a preparation method thereof:

[0043] (1) Preparation of crude extract: 50 g of Cornus officinalis powder was taken, and the Cornus officinalis powder was mixed with an extraction solvent (the volume ratio of ethanol, glycerol and acetone was 1:3:5) at a solid-liquid ratio of 1:15 (g / mL), and ultrasonic-assisted extraction and microwave-assisted extraction were performed simultaneously. The extraction was performed at 35°C, the power of ultrasonic-assisted extraction was 80 W, the extraction time was 40 min, and the power of microwave-assisted extraction was 700 W, and the extraction time was 40 min. The crude extract was filtered with a 0.22 μm filter membrane, and the filtrate was the Cornus officinalis crude extract;

[0044] (2) Concentration: The crude extract of Cornus officinalis is concentrated by rotary evaporation, and then freeze-dried to obtain the Cornus officinalis extract.

[0045] Example 2

[0046] This embodiment provides a cornus officinalis extract and a preparation method thereof:

[0047] (1) Preparation of crude extract: 50 g of Cornus officinalis powder was taken, and the Cornus officinalis powder was mixed with an extraction solvent (the volume ratio of ethanol, glycerol and acetone was 3:5:8) at a solid-liquid ratio of 1:30 (g / mL), and ultrasonic-assisted extraction and microwave-assisted extraction were performed simultaneously. The extraction was performed at 40°C, the power of ultrasonic-assisted extraction was 100 W, the extraction time was 30 min, and the power of microwave-assisted extraction was 600 W, and the microwave-assisted extraction time was 30 min. The crude extract was filtered with a 0.22 μm filter membrane, and the filtrate was the Cornus officinalis crude extract;

[0048] (2) Concentration: The crude extract of Cornus officinalis is concentrated by rotary evaporation, and then freeze-dried to obtain the Cornus officinalis extract.

[0049] Example 3

[0050] This embodiment provides a cornus officinalis extract and a preparation method thereof:

[0051] (1) Preparation of crude extract: 50 g of cornus officinalis powder was taken, and the cornus officinalis powder was mixed with an extraction solvent (the volume ratio of ethanol, glycerol and acetone was 2:3:5) at a solid-liquid ratio of 1:9 (g / mL), and ultrasonic-assisted extraction and microwave-assisted extraction were performed simultaneously. The extraction was performed at 35°C, the power of ultrasonic-assisted extraction was 120 W, the extraction time was 20 min, and the power of microwave-assisted extraction was 500 W, and the microwave-assisted extraction time was 20 min. The crude extract was filtered with a 0.22 μm filter membrane, and the filtrate was the cornus officinalis crude extract;

[0052] (2) Concentration: The crude extract of Cornus officinalis is concentrated by rotary evaporation, and then freeze-dried to obtain the Cornus officinalis extract.

[0053] Example 4

[0054] This embodiment provides a Cornus officinalis extract and a preparation method thereof. The preparation method is different from that of Example 1 only in that the formula of the extraction solvent is different, the extraction solvent does not contain acetone, and the volume ratio of ethanol and glycerol and the amount of the extraction solvent remain unchanged, and other conditions remain unchanged.

[0055] Example 5

[0056] This embodiment provides a Cornus officinalis extract and a preparation method thereof. The preparation method is different from that of Example 1 only in that the formula of the extraction solvent is different, the extraction solvent does not contain glycerol, and the volume ratio of ethanol and acetone and the amount of the extraction solvent remain unchanged, and other conditions remain unchanged.

[0057] Example 6

[0058] This embodiment provides a Cornus officinalis extract and a preparation method thereof. The preparation method is different from that of Example 1 only in that the formula of the extraction solution is different, the extraction solvent does not contain ethanol, and the volume ratio of glycerol to acetone and the amount of the extraction solvent remain unchanged, and other conditions remain unchanged.

[0059] Example 7

[0060] This embodiment provides a Cornus officinalis extract and a preparation method thereof. The preparation method is different from that of Example 1 only in that microwave-assisted extraction is not performed, and the power of ultrasonic-assisted extraction is 110 W, the extraction time is 40 min, and other conditions remain unchanged.

[0061] Example 8

[0062] This embodiment provides a Cornus officinalis extract and a preparation method thereof. The preparation method is different from that of Example 1 only in that ultrasound-assisted extraction is not performed, the power of microwave-assisted extraction is 900 W, the extraction time is 40 min, and other conditions remain unchanged.

[0063] Comparative Example 1

[0064] The comparative example provides a cornus officinalis extract and a preparation method thereof. The preparation method comprises the following steps: taking 50 g of cornus officinalis powder, mixing the cornus officinalis powder with an extraction solvent (80% ethanol aqueous solution) at a solid-liquid ratio (g / mL) of 1:15, stirring at 60° C. and 160 rpm for 40 min, filtering the crude extract with a 0.22 μm filter membrane, and obtaining a cornus officinalis crude extract; concentrating the cornus officinalis crude extract by rotary evaporation, and then freeze-drying the cornus officinalis extract.

[0065] Comparative Example 2

[0066] The comparative example provides a cornus officinalis extract and a preparation method thereof. The preparation method comprises the following steps: taking 50 g of cornus officinalis powder, mixing the cornus officinalis powder with an extraction solvent (80% ethanol aqueous solution) at a solid-liquid ratio (g / mL) of 1:15, subjecting the mixture to electric shock for 200 times at a voltage of 7000 V, filtering the mixture with a 0.22 μm filter membrane, and obtaining a cornus officinalis crude extract; concentrating the cornus officinalis crude extract by rotary evaporation, and then freeze-drying the mixture to obtain the cornus officinalis extract.

[0067] Comparative Example 3

[0068] The comparative example provides a cornus officinalis extract and a preparation method thereof. The preparation method comprises the following steps: taking 50 g of cornus officinalis powder, mixing the cornus officinalis powder with an extraction solvent (80% ethanol aqueous solution) at a solid-liquid ratio (g / mL) of 1:15, homogenizing at 1000 bar for 4 min, stirring at 60° C. for 20 min, filtering with a 0.22 μm filter membrane, and obtaining a cornus officinalis crude extract; concentrating the cornus officinalis crude extract by rotary evaporation, and then freeze-drying the cornus officinalis extract.

[0069] Comparative Example 4

[0070] The comparative example provides a cornus officinalis extract and a preparation method thereof. The preparation method comprises the following steps: taking 50 g of cornus officinalis powder, mixing the cornus officinalis powder with an extraction solvent (80% ethanol aqueous solution) at a solid-liquid ratio (g / mL) of 1:15, extracting for 1.5 h in a reflux device at 74° C., filtering with a 0.22 μm filter membrane, and obtaining a filtrate that is a cornus officinalis crude extract; concentrating the cornus officinalis crude extract by rotary evaporation, and then freeze-drying the cornus officinalis extract.

[0071] Test Example 1

[0072] The Cornus officinalis extracts obtained in Examples 1-8 and Comparative Examples 1-4 were subjected to HPLC analysis. The conditions for HPLC analysis were as follows:

[0073] Sample processing method:

[0074] Each group of samples was dissolved in methanol, filtered through a 0.45um filter membrane, and the filtrate was used as the test solution.

[0075] Liquid chromatography conditions:

[0076] Chromatographic column: Eclipse XDB-C18 Column (4.6×150mm×5μm);

[0077] Mobile phase ratio: V (mobile phase A-methanol) 87%, V (mobile phase B-0.1% trifluoroacetic acid aqueous solution) 13%; flow rate: 1.0 mL / min; detection wavelength: 210 nm; column temperature: 30°C; injection volume: 10 μL.

[0078] The contents of ursolic acid in the Cornus officinalis extracts obtained in Examples 1-8 and Comparative Examples 1-4 are shown in Table 1.

[0079] Table 1

[0080]

[0081]

[0082] It can be seen from the data in Table 1 that compared with other extraction processes, the Cornus officinalis extract prepared by the preparation method of the present invention has a higher content of ursolic acid, and the use of ultrasound-assisted extraction combined with microwave-assisted extraction can better increase the content of ursolic acid. At the same time, the formula of the extraction solvent also affects the above effect.

[0083] Test Example 2

[0084] Effect of Cornus officinalis extract on the survival rate of nematodes under ultraviolet irradiation:

[0085] After the nematodes were lysed, the eggs were dropped onto NGM plates containing different concentrations (0 mg / mL, 1 mg / mL, 2 mg / mL, and 4 mg / mL) of the Cornus officinalis extract samples prepared in Example 1. During the egg-laying period of the nematodes, the nematodes were transferred to a new drug plate every day to prevent the eggs from hatching into larvae and affecting the experiment. At the end of the egg-laying period, the nematodes were picked onto a blank NGM plate. For the lifespan test, about 70 nematodes were placed on each plate, with 3 parallel groups in each group, and placed in a UV crosslinker for irradiation (UVB 100 mJ / cm 2 ). After irradiation, the nematodes were transferred to a plate containing E. coli OP50 solution. The time of picking nematodes after irradiation was recorded as day 0. During the entire experimental period, the number of nematodes was counted every 24 hours. When all the nematodes died, the counting was stopped, and the lifespan curve was drawn. The relative lifespan was calculated (with the control group 0 mg / mL as 100%). The results are shown as follows: Figure 1 and Figure 2 shown.

[0086] according to Figure 1 and Figure 2 It can be seen that the three concentrations of Cornus officinalis extract (1 mg / mL, 2 mg / mL, and 4 mg / mL, respectively) can significantly increase the lifespan of nematodes under UVB irradiation.

[0087] According to the above method, the relative lifespan (%) of the Cornus officinalis extracts prepared in Examples 1-8 and Comparative Examples 1-4 at 4 mg / mL was simultaneously measured, as shown in Table 2.

[0088] Table 2

[0089] Group Relative life span (%) Control group 100％ Example 1 120.12％ Example 2 122.24％ Example 3 120.35％ Example 4 116.32％ Example 5 112.27％ Example 6 114.43％ Example 7 104.13％ Example 8 106.27％ Comparative Example 1 106.78％ Comparative Example 2 109.47％ Comparative Example 3 102.13％ Comparative Example 4 105.37％

[0090] It can be seen from the data in Table 2 that compared with other extraction processes, the Cornus officinalis extract prepared by the preparation method of the present invention has a better anti-photoaging effect, and the selection of the extraction method and the formula of the extraction solvent will affect the above effect.

[0091] Test Example 3

[0092] Effect of Cornus officinalis extract on the ultraviolet radiation (UVB 100mJ / cm 2 ) Effects of reactive oxygen levels in nematodes:

[0093] Chronic UVB irradiation can induce excessive reactive oxygen free radicals (ROS) in the skin. Excessive ROS can destroy the balance of the skin's oxidative defense system, ultimately leading to skin oxidative stress and photoaging. Therefore, the level of ROS in the nematodes after irradiation can reflect the photoaging condition. The ROS level in the nematodes of Example 1 in Test Example 2 was detected using a fluorescent probe, and the relative fluorescence intensity of the nematodes was analyzed using Image J software. The results are as follows: Figure 3 and Figure 4 shown.

[0094] Compared with the control group without adding Cornus officinalis extract, the fluorescence intensity of ROS in nematodes in the groups intervened with Cornus officinalis extract at three concentrations was significantly reduced, showing a dose-dependent effect, indicating that the Cornus officinalis extract prepared by the present invention can remove excessive ROS in nematodes and improve the oxidative damage state.

[0095] Test Example 4

[0096] Effect of Cornus officinalis extract on the ultraviolet radiation (UVB 100mJ / cm 2 ) Effects of antioxidant enzyme activities in nematodes:

[0097] Superoxide dismutase (SOD) can catalyze superoxide anion free radicals to generate oxygen and hydrogen peroxide, thereby exerting an antioxidant effect; malondialdehyde (MDA) is the final metabolite of one of the main products of lipid peroxidation damage and the numerous unsaturated aldehydes and ketones produced by lipid peroxidation. The three groups of nematodes treated with the cornus extract obtained in Test Example 2 were collected and ground to obtain tissue fluid, and the supernatant was taken to determine the SOD activity and MDA content.

[0098] The results are as follows Figure 5 and Figure 6 As shown, compared with the control group, the group treated with Cornus officinalis extract increased the superoxide dismutase activity, reduced the malondialdehyde content, and enhanced the ability of nematodes to resist oxidative stress.

[0099] Test Example 5

[0100] Effect of Cornus officinalis extract on the ultraviolet radiation (UVB 100mJ / cm 2) The impact of related genes in nematodes:

[0101] The nematodes treated with the Cornus officinalis extract obtained in Test Example 2 were subjected to 100 mJ / cm 2 After UVB irradiation, the nematodes obtained in Test Example 2 were collected, and 1 mL of Trizol solution was added to extract total RNA, and then placed in a grinder to grind into tissue fluid. 0.2 mL of chloroform was added to each sample, vortexed for 30 seconds, allowed to stand at room temperature for 3 minutes, and then centrifuged (4°C, 12,000 rpm, 15 minutes). Note: At this time, the sample will present three layers, and the supernatant RNA layer (about 400-450 μL) was taken to a new EP tube. Then, an equal volume of isopropanol was added, gently mixed, and after standing for 10 minutes, centrifuged (4°C, 12000 rpm, 15 minutes), the supernatant was discarded, and a small amount of white precipitate (ie, RNA) was obtained. Then 75% anhydrous ethanol (about 1 mL) was added to wash the obtained sample to remove isopropanol impurities, centrifuged (4°C, 14,000 rpm, 5 minutes), the supernatant was discarded, and it was dried naturally. Finally, an appropriate amount of DEPC water was added to completely dissolve the RNA. Note: Determine OD 260 / 280 ratio (in the range of 1.8–2.0), cDNA was synthesized by a reverse transcription kit (Takara, Japan), and primers of related genes were added for fluorescence quantification.

[0102] The results are as follows Figure 7 As shown in the figure (the columns from left to right are the results of 0 mg / mL, 1 mg / mL, 2 mg / mL, and 4 mg / mL), the Cornus officinalis extract involved in the present invention can significantly increase the expression of stress resistance genes mev-1 and isp-1 genes, the expression of antioxidant gene sod-3 gene, and the expression of sek-1, skn-1, gcs-1, gst-4, and gst-7 genes in the skn-1 pathway in nematodes after UVB irradiation.

[0103] The applicant declares that the present invention illustrates the detailed process flow of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned detailed process flow, that is, it does not mean that the present invention must rely on the above-mentioned detailed process flow to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A method for increasing the content of ursolic acid in a cornus officinalis extract, characterized in that: The preparation method comprises the following steps: (1) Mixing Cornus officinalis with an extraction solvent for extraction, centrifuging and filtering to obtain a sample solution; The extraction solvent is a combination of ethanol, glycerol and acetone; the volume ratio of ethanol, glycerol and acetone is (1-4): (3-6): (5-10); The extraction method is a combination of ultrasound-assisted extraction and microwave-assisted extraction; the extraction time is independently 20-40 minutes; the power of the ultrasound-assisted extraction is 80W-115W; the power of the microwave-assisted extraction is 530-700W; (2) The sample solution is concentrated and dried to obtain a Cornus officinalis extract.

2. The method for preparing a cornus officinalis extract for increasing the content of ursolic acid according to claim 1, characterized in that: The mass volume ratio of the Cornus officinalis and the extraction solvent is (2-6): (50-70) g / mL.

3. The method for preparing the method for increasing the content of ursolic acid in the Cornus officinalis extract according to claim 1, characterized in that: The ultrasound-assisted extraction and microwave-assisted extraction are performed simultaneously.

4. The method for preparing the method for increasing the ursolic acid content in the Cornus officinalis extract according to claim 1, characterized in that: The extraction temperature is 10-50°C.

5. The method for preparing the method for increasing the content of ursolic acid in the Cornus officinalis extract according to claim 1, characterized in that: The concentration includes any one of rotary evaporation, precipitation or recrystallization or a combination of at least two thereof.

6. The method for preparing the method for increasing the ursolic acid content in the Cornus officinalis extract according to claim 1, characterized in that: The drying includes any one of freeze drying, boiling drying or spray drying, or a combination of at least two of them.

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