Liquid monitoring structure, microfluidic chip and detection system

Abstract

This application relates to a liquid monitoring structure, a microfluidic chip, and a detection system. The microfluidic chip includes a chip body and the liquid monitoring structure. The liquid monitoring structure includes a quality control chamber, a liquid inlet passage, and a liquid outlet passage. The quality control chamber contains a detection reagent; one end of the liquid inlet passage is connected to the quality control chamber, and the other end of the liquid inlet passage is used to connect to other liquid paths of the microfluidic chip to introduce the liquid to be detected into the quality control chamber to mix and react with the detection reagent; the liquid outlet passage is connected to the quality control chamber. The liquid monitoring structure has a first state and a second state. In the first state, the liquid outlet passage is closed, and the detection liquid in the quality control chamber can mix and react with the detection reagent; in the second state, the liquid outlet passage is open, and the liquid outlet passage can drain the mixture in the quality control chamber, while the quality control chamber can retain at least a portion of the mixture.

Description

Technical Field

[0001] This invention relates to the field of medical technology, and in particular to a liquid monitoring structure, a microfluidic chip, and a detection system. Background Technology

[0002] Microfluidic chips are microanalytical systems that integrate sample pretreatment, mixing, reaction, separation, and detection into one or more chips, replacing traditional laboratory procedures. Microfluidic chips offer advantages such as small sample volumes, simple operation, and the ability to accurately complete the entire process from sample preparation to result display in a short time, effectively overcoming experimental errors caused by manual operations in traditional laboratory work. Therefore, microfluidic chips are increasingly used in fields such as chemical analysis, DNA sequencing, protein analysis, single-cell analysis, single-molecule analysis, food safety, environmental monitoring, and drug screening.

[0003] However, some traditional immunomicrofluidic chips can only monitor the liquid path once, and some traditional immunomicrofluidic chips have no quality control at all. Immunoassay requires multiple liquid transfer processes such as incubation, washing, and substrate addition, making it impossible to accurately determine whether the liquid at each node has been accurately transferred to the preset position, and thus unable to effectively monitor the operation of the liquid path.

[0004] The information disclosed above in the background art of this application is only for understanding the background of the concept of this application, and may contain information that does not constitute prior art. Summary of the Invention

[0005] Therefore, it is necessary to provide a liquid monitoring structure, microfluidic chip, and detection system to address the above problems.

[0006] A liquid monitoring structure for a microfluidic chip, comprising:

[0007] A quality control chamber containing testing reagents;

[0008] The liquid inlet passage has one end connected to the quality control chamber and the other end connected to other liquid paths of the microfluidic chip to introduce the liquid to be tested into the quality control chamber to mix and react with the detection reagent.

[0009] A drainage passage, which is connected to the quality control chamber;

[0010] The liquid monitoring structure has a first state and a second state. In the first state, the drain passage is closed, and the detection liquid in the quality control chamber can mix and react with the detection reagent. In the second state, the drain passage is open, and the drain passage can drain the mixture in the quality control chamber, while the quality control chamber can retain at least a portion of the mixture.

[0011] Before describing the technical effects achievable by the embodiments of this application, it should be noted that some traditional immune microfluidic chips can only monitor the liquid path once, and some traditional immune microfluidic chips have no quality control at all. Immunological detection requires multiple liquid transfer processes such as incubation, cleaning, and substrate addition, making it impossible to accurately determine whether the liquid at each node has been accurately transferred to the preset position, and thus unable to effectively monitor the operation of the liquid path.

[0012] To address the aforementioned problems, the liquid monitoring structure of the microfluidic chip described in this application can achieve at least the following beneficial effects: the liquid inlet passage of the liquid monitoring structure can be connected to a certain liquid path of the microfluidic chip to detect whether liquid flows through that liquid path. This liquid path can be considered the monitored liquid path. When liquid flows through this liquid path, at least a portion of the liquid will flow into the quality control chamber through the liquid inlet passage. That is, the liquid flowing through the liquid path is the test liquid mentioned above. When the test liquid enters the quality control chamber, the drain passage is in its first state, i.e., the drain passage is closed. In this way, the test liquid can mix and react with the detection reagent in the quality control chamber, instead of being directly discharged from the drain passage. The detection reagent can detect signals, such as one or more of the following signals: luminescence signal, fluorescence signal, absorption signal, color development signal, etc. By mixing and reacting the detection reagent with the test liquid, it is possible to detect whether liquid flows through the monitored liquid path, thereby accurately determining whether the liquid has been accurately transferred to the preset position. After the current test is completed, the drainage path enters its second state, meaning the drainage path is open, allowing most of the mixture in the control chamber to drain. The control chamber can also retain at least a portion of the mixture. It is important to emphasize that leaving a portion of the mixture in the control chamber allows the detection reagents contained within it to be used during the next monitoring cycle. This ensures that when the next liquid to be tested flows into the control chamber, there are still detection reagents with detection effectiveness present, guaranteeing the effectiveness of the detection and monitoring. In other words, the liquid monitoring structure of the microfluidic chip in this application does not perform one-time monitoring of a specific liquid path, but rather allows for multiple liquid path monitoring cycles by consistently retaining a portion of detection reagents with detection effectiveness in the control chamber.

[0013] In one embodiment, the quality control chamber includes a main chamber and a retention chamber connected to the main chamber. The main chamber is connected to one end of the inlet passage, and the bottom of the main chamber is connected to the drain passage. In the second state, the drain passage is open, allowing the mixed liquid in the main chamber to be discharged. The retention chamber is used to retain the mixed liquid. When the drain passage is in the first state, it is closed, allowing the test solution to mix and react with the test reagent in the main chamber and the retention chamber of the quality control chamber. After the reaction and detection are completed, the drain passage switches to the second state, i.e., the drain passage is open, allowing the mixed solution of the test solution and the test reagent in the main chamber to be discharged from the drain passage. However, some of the mixed solution can remain in the retention chamber for mixing and reaction with the next test solution, thus achieving the effect of monitoring the detection liquid path.

[0014] In one embodiment, in the drainage direction of the drainage passage, the retention chamber is at least partially lower than the inlet cross-section of the drainage passage. The drainage direction can be considered as the flow direction of the liquid in the quality control chamber as it is discharged from the drainage passage, or as the length extension direction of the drainage passage. This structural arrangement can better ensure that a portion of the mixture containing the test reagent is always retained in the retention chamber, rather than flowing out completely from the drainage passage.

[0015] In one embodiment, the liquid monitoring structure further includes an overflow path, one end of which is connected to the side wall of the main cavity. When too much test liquid enters the main cavity of the quality control chamber, there is a risk that the test liquid will overflow onto the chip surface, affecting the entire testing process and results. To avoid this situation, an overflow path connected to the main cavity is added here. When there is too much test liquid or too much mixture of test liquid and test reagent, it can flow out in time through this overflow path, thereby preventing the liquid in the quality control chamber from overflowing onto the chip surface.

[0016] In one embodiment, the liquid monitoring structure further includes an overflow chamber, the other end of which is connected to the overflow passage. The overflow chamber can collect liquid flowing out from the overflow passage.

[0017] In one embodiment, a control valve is provided within the drainage passage to control the opening and closing of the drainage passage. The control valve controls the opening and closing of the drainage passage to switch the liquid monitoring structure between a first state and a second state.

[0018] In one embodiment, the control valve includes at least one of a capillary valve, a hydrophilic valve, a hydrophobic valve, and a siphon valve.

[0019] In one embodiment, the liquid monitoring structure further includes a drainage chamber, one end of which is connected to the bottom of the quality control chamber, and the other end of which is connected to the drainage chamber. The drainage chamber can collect liquid discharged from the drainage passage.

[0020] This application also provides a microfluidic chip, which includes a chip body and a liquid monitoring structure as described in any of the above embodiments.

[0021] This application also provides a detection system, which includes the microfluidic chip described in any of the above embodiments.

[0022] The aforementioned microfluidic chip and detection system, including the liquid monitoring structure described in any of the above embodiments, also possess at least the following beneficial effects: the liquid inlet passage of the liquid monitoring structure can be connected to a liquid path of the microfluidic chip to detect whether liquid flows through that liquid path. This liquid path can be considered the monitored liquid path. When liquid flows through this liquid path, at least a portion of the liquid will flow into the quality control chamber through the liquid inlet passage. That is, the liquid flowing through the liquid path is the test liquid mentioned above. When the test liquid enters the quality control chamber, the drain passage is in its first state, i.e., the drain passage is closed. Thus, the test liquid can mix and react with the detection reagent in the quality control chamber without being directly discharged from the drain passage. The detection reagent can detect signals, such as one or more of the following: luminescence signal, fluorescence signal, absorbance signal, color development signal, etc. By mixing and reacting the detection reagent with the test liquid, it is possible to detect whether liquid flows through the monitored liquid path, thereby accurately determining whether the liquid has been accurately transferred to the preset position. After the current test is completed, the drainage path enters its second state, meaning the drainage path is open, allowing most of the mixture in the control chamber to drain. The control chamber can also retain at least a portion of the mixture. It is important to emphasize that leaving a portion of the mixture in the control chamber allows the detection reagents contained within it to be used during the next monitoring cycle. This ensures that when the next liquid to be tested flows into the control chamber, there are still detection reagents with detection effectiveness present, guaranteeing the effectiveness of the detection and monitoring. In other words, the liquid monitoring structure of the microfluidic chip in this application does not perform one-time monitoring of a specific liquid path, but rather allows for multiple liquid path monitoring cycles by consistently retaining a portion of detection reagents with detection effectiveness in the control chamber. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of this application or the conventional technology, the drawings used in the description of the embodiments or the conventional technology will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of a liquid monitoring structure provided in one embodiment of the present invention.

[0025] Figure 2 This is a schematic diagram of a liquid monitoring structure provided in another embodiment of the present invention.

[0026] Figure label:

[0027] 10. Liquid monitoring structure; 100. Quality control chamber; 110. Main chamber; 120. Liquid retention chamber; 200. Liquid inlet passage; 300. Liquid drain passage; 310. Control valve; 400. Overflow passage; 500. Overflow chamber; 600. Liquid drain chamber. Detailed Implementation

[0028] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0029] Please see Figure 1 and Figure 2 In some embodiments, this application provides a liquid monitoring structure 10 for a microfluidic chip, comprising a quality control chamber 100, a liquid inlet passage 200, and a liquid outlet passage 300. The quality control chamber 100 contains a detection reagent; one end of the liquid inlet passage 200 is connected to the quality control chamber 100, and the other end of the liquid inlet passage 200 is used to connect to other liquid paths of the microfluidic chip to introduce a test liquid into the quality control chamber 100 to mix and react with the detection reagent; the liquid outlet passage 300 is connected to the quality control chamber 100. The liquid monitoring structure 10 has a first state and a second state. In the first state, the liquid outlet passage 300 is closed, and the test liquid in the quality control chamber 100 can mix and react with the detection reagent; in the second state, the liquid outlet passage 300 is open, and the liquid outlet passage 300 can drain the mixture in the quality control chamber 100, while the quality control chamber 100 can retain at least a portion of the mixture.

[0030] Before describing the technical effects achievable by the embodiments of this application, it should be noted that some traditional immune microfluidic chips can only monitor the liquid path once, and some traditional immune microfluidic chips have no quality control at all. Immunological detection requires multiple liquid transfer processes such as incubation, cleaning, and substrate addition, making it impossible to accurately determine whether the liquid at each node has been accurately transferred to the preset position, and thus unable to effectively monitor the operation of the liquid path.

[0031] To address the aforementioned problems, the liquid monitoring structure 10 of the microfluidic chip described in this application can achieve at least the following beneficial effects: the liquid inlet passage 200 of the liquid monitoring structure 10 can be connected to a certain liquid path of the microfluidic chip to detect whether liquid flows through that liquid path. This liquid path can be considered as the monitored liquid path. When liquid flows through this liquid path, at least a portion of the liquid will flow into the quality control chamber 100 through the liquid inlet passage 200. That is, the liquid flowing through the liquid path is the test liquid mentioned above. When the test liquid enters the quality control chamber 100, the drain passage 300 is in its first state, i.e., the drain passage 300 is closed. In this way, the test liquid can mix and react with the detection reagent in the quality control chamber 100, instead of being directly discharged from the drain passage 300. The detection reagent can detect signals, such as one or more of the following signals: luminescence signal, fluorescence signal, absorption signal, and colorimetric signal. By mixing and reacting the test reagent with the test liquid, the presence of liquid flowing through the monitored liquid path can be detected, thus accurately determining whether the liquid has been accurately transferred to the preset position. After the detection is completed, the drain passage 300 enters a second state, i.e., the drain passage 300 is open, allowing most of the mixture in the quality control chamber 100 to be drained. The quality control chamber 100 can also retain at least a portion of the mixture. It is important to emphasize that leaving a portion of the mixture in the quality control chamber 100 allows the test reagent contained within it to be used in the next monitoring. That is, when the test liquid flows into the quality control chamber 100 again, it ensures that there is still a test reagent with detection effect in the quality control chamber 100, ensuring the effectiveness of detection and monitoring. In other words, the liquid monitoring structure 10 of the microfluidic chip in this application does not perform one-time monitoring of a specific liquid path, but rather can always retain a portion of the test reagent with detection effect in the quality control chamber 100 to achieve multiple liquid path monitoring.

[0032] Specifically, such as Figure 1 and Figure 2As shown, in some embodiments, the quality control chamber 100 includes a main chamber 110 and a retention chamber 120 connected to the main chamber 110. The main chamber 110 is connected to one end of the inlet passage 200, and the bottom of the main chamber 110 can be connected to the drain passage 300. In the second state, the drain passage 300 is opened, and the drain passage 300 can discharge the mixed liquid in the main chamber 110. The retention chamber 120 is used to retain the mixed liquid. When the drain passage 300 is in the first state, the drain passage 300 is closed, and the test solution can be mixed and reacted with the test reagent in the main chamber 110 and the retention chamber 120 of the quality control chamber 100. After the reaction and detection are completed, the drain passage 300 switches to the second state, that is, the drain passage 300 is opened, and the mixture of the test solution and the test reagent in the main chamber 110 can be discharged from the drain passage 300. However, some of the mixture can still be retained in the retention chamber 120, so that it can be mixed and reacted with the test solution again when it enters next time, so as to achieve the effect of monitoring the detection liquid path.

[0033] More specifically, such as Figure 1 and Figure 2 As shown, in some embodiments, the shape of the retention chamber 120 is not limited, as long as it can retain the test reagent. For example, the cross-sectional profile of the retention chamber 120 can be circular, elliptical, square, rectangular, irregular, or other shapes. In the drainage direction of the drainage passage 300, the retention chamber 120 is at least partially lower than the inlet cross-section of the drainage passage 300. The drainage direction can be considered as the flow direction of the liquid in the quality control chamber 100 when it is discharged from the drainage passage 300, or it can be considered as the length extension direction of the drainage passage 300. This structural arrangement can better ensure that a portion of the mixture containing the test reagent is always retained in the retention chamber 120, and does not flow out completely from the drainage passage 300.

[0034] Furthermore, such as Figure 1 and Figure 2 As shown, in some embodiments, a control valve 310 is provided within the drainage passage 300, which is used to control the opening and closing of the drainage passage 300. The control valve 310 controls the opening and closing of the drainage passage 300 to achieve switching of the liquid monitoring structure 10 between a first state and a second state.

[0035] Furthermore, such as Figure 1 and Figure 2 As shown, in some embodiments, the control valve 310 includes at least one of a capillary valve, a hydrophilic valve, a hydrophobic valve, and a siphon valve.

[0036] Furthermore, such as Figure 1 and Figure 2As shown, in some embodiments, the liquid monitoring structure 10 further includes a drainage chamber 600. One end of the drainage passage 300 is connected to the bottom of the quality control chamber 100, and the other end of the drainage passage 300 is connected to the drainage chamber 600. The shape of the drainage chamber 600 is not limited; its cross-sectional profile can be circular, elliptical, square, rectangular, irregular, or other shapes. The drainage chamber 600 can collect the liquid discharged from the drainage passage 300.

[0037] Specifically, such as Figure 2 As shown, in some embodiments, the liquid monitoring structure 10 further includes an overflow path 400, one end of which is connected to the side wall of the main cavity 110. The shape of the overflow channel, such as the overflow path 400, can be a straight line, a broken line, or a curve. When too much test liquid enters the main cavity 110 of the quality control cavity 100, there is a risk that the test liquid will overflow onto the chip surface, affecting the entire detection process and results. To avoid this situation, an overflow path 400 connected to the main cavity 110 is added here. When there is too much test liquid or too much mixture of test liquid and test reagent, it can flow out in time through the overflow path 400, thereby preventing the liquid in the quality control cavity 100 from overflowing onto the chip surface.

[0038] More specifically, such as Figure 2 As shown, in some embodiments, the liquid monitoring structure 10 further includes an overflow cavity 500, the other end of the overflow passage 400 being connected to the overflow cavity 500. The overflow cavity 500 can collect liquid flowing out from the overflow passage 400. The shape of the overflow cavity 500 is not limited; its cross-sectional profile can be circular, elliptical, square, rectangular, irregular, or other shapes.

[0039] In addition, this application also provides a microfluidic chip, which includes a chip body (not shown) and a liquid monitoring structure 10 as described in any of the above embodiments.

[0040] This application also provides a detection system, which includes the microfluidic chip described in any of the above embodiments.

[0041] The aforementioned microfluidic chip and detection system, including the liquid monitoring structure 10 described in any of the above embodiments, also have at least the following beneficial effects: the liquid inlet passage 200 of the liquid monitoring structure 10 can be connected to a certain liquid path of the microfluidic chip to detect whether liquid flows through that liquid path. This liquid path can be considered as the monitored liquid path. When liquid flows through this liquid path, at least a portion of the liquid will flow into the quality control chamber 100 through the liquid inlet passage 200. That is, the liquid flowing through the liquid path is the test liquid mentioned above. When the test liquid enters the quality control chamber 100, the drain passage 300 is in the first state, that is, the drain passage 300 is closed. In this way, the test liquid can mix and react with the detection reagent in the quality control chamber 100, instead of being directly discharged from the drain passage 300. The detection reagent can detect signals, such as one or more of the following signals: luminescence signal, fluorescence signal, absorption signal, and colorimetric signal. By mixing and reacting the test reagent with the test liquid, the presence of liquid flowing through the monitored liquid path can be detected, thus accurately determining whether the liquid has been accurately transferred to the preset position. After the detection is completed, the drain passage 300 enters a second state, i.e., the drain passage 300 is open, allowing most of the mixture in the quality control chamber 100 to be drained. The quality control chamber 100 can also retain at least a portion of the mixture. It is important to emphasize that leaving a portion of the mixture in the quality control chamber 100 allows the test reagent contained within it to be used in the next monitoring. That is, when the test liquid flows into the quality control chamber 100 again, it ensures that there is still a test reagent with detection effect in the quality control chamber 100, ensuring the effectiveness of detection and monitoring. In other words, the liquid monitoring structure 10 of the microfluidic chip in this application does not perform one-time monitoring of a specific liquid path, but rather can always retain a portion of the test reagent with detection effect in the quality control chamber 100 to achieve multiple liquid path monitoring.

[0042] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0043] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

[0044] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0045] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0046] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0047] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0048] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0049] In the description of this specification, references to terms such as "an embodiment," "another implementation," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative descriptions of the above terms do not necessarily refer to the same embodiment or example. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of this application.

Claims

1. A liquid monitoring structure for a microfluidic chip, characterized in that, include: A quality control chamber containing test reagents, the quality control chamber including a main chamber and a retention chamber communicating with the main chamber; The liquid inlet passage has one end connected to the main cavity and the other end connected to other liquid paths of the microfluidic chip to introduce the liquid to be tested into the quality control cavity to mix and react with the detection reagent. A drainage passage, which is connected to the bottom of the main cavity; The liquid monitoring structure has a first state and a second state. In the first state, the drain passage is closed, and the detection liquid in the quality control chamber can mix and react with the detection reagent. In the second state, the drainage passage is opened, which can drain the mixture in the main chamber, and the retention chamber is used to retain the mixture.

2. The liquid monitoring structure of the microfluidic chip according to claim 1, characterized in that, In the drainage direction of the drainage passage, the retention chamber is at least partially lower than the inlet cross-section of the drainage passage.

3. The liquid monitoring structure of the microfluidic chip according to claim 1, characterized in that, The liquid monitoring structure also includes an overflow path, one end of which is connected to the side wall of the main cavity.

4. The liquid monitoring structure of the microfluidic chip according to claim 3, characterized in that, The liquid monitoring structure also includes an overflow cavity, and the other end of the overflow path is connected to the overflow cavity.

5. The liquid monitoring structure of the microfluidic chip according to any one of claims 1 to 4, characterized in that, The drainage passage is equipped with a control valve, which is used to control the opening and closing of the drainage passage.

6. The liquid monitoring structure of the microfluidic chip according to claim 5, characterized in that, The control valve includes at least one of a capillary valve, a hydrophilic valve, a steam trap, and a siphon valve.

7. The liquid monitoring structure of the microfluidic chip according to any one of claims 1 to 4, characterized in that, The liquid monitoring structure also includes a drainage chamber, one end of which is connected to the bottom of the quality control chamber, and the other end of which is connected to the drainage chamber.

8. A microfluidic chip, characterized in that, It includes the chip body and the liquid monitoring structure as described in any one of claims 1 to 7.

9. A detection system, characterized in that, Including the microfluidic chip as described in claim 8.

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