Microfluidic chip and microfluidic device

Abstract

The application provides a microfluidic chip and a microfluidic device. The microfluidic chip comprises: a substrate comprising a detection area and a non-detection area, the non-detection area being arranged around the detection area; a grounding trace and a driving power supply trace arranged in the non-detection area and respectively surrounding the detection area; a binding part arranged in the non-detection area, the grounding trace and the driving power supply trace being electrically connected to the binding part; and a breakage detection line arranged in the non-detection area, two ends of the breakage detection line being respectively electrically connected to the binding part, and the breakage detection line extending from a first end to a second end around the detection area. The impedance value between the first end and the second end is detected through the binding part, or a detection signal is input at the first end and a feedback signal is received at the second end through the binding part, so as to detect whether the microfluidic chip is broken. The microfluidic chip can realize breakage detection of the chip, so as to prevent biochemical reagents from being invalidly used and biochemical detection cost from being increased.

Description

TECHNICAL FIELD

[0001] The present application relates to the microfluidic technology field, in particular to a microfluidic chip and a microfluidic device. BACKGROUND

[0002] The microfluidic technology is a kind of technology for manipulating droplets by dielectric wetting, which can realize the generation, movement, mixing and splitting of droplets, and can realize the construction of a universal biochemical analysis platform by manipulating droplets with different chemical components combined with optical, electrochemical, immune reaction and other technologies.

[0003] At present, the microfluidic technology is still a new technology, and the microfluidic chip as a consumable has high cost, and as a high-precision biochemical analysis platform, the biochemical reagent has high unit price, so the consumption needs to be strictly controlled. Generally, the microfluidic chip adopts a glass substrate, which is easy to break, and if the experiment analysis is carried out on the broken microfluidic chip, the biochemical reagent will be used invalidly, and the detection cost will be increased. SUMMARY

[0004] The present application provides a microfluidic chip and a microfluidic device, which aims to solve the problem of invalid use of biochemical reagents and high detection cost caused by using broken microfluidic chips in the prior art.

[0005] In order to solve the above technical problems, the first technical solution provided by the present application is to provide a microfluidic chip. The microfluidic chip comprises:

[0006] A substrate, the substrate comprises a detection area and a non-detection area, and the non-detection area is arranged around the detection area;

[0007] A ground trace and a driving power supply trace are arranged in the non-detection area and surround the detection area respectively;

[0008] A binding part is arranged in the non-detection area, and the ground trace and the driving power supply trace are electrically connected to the binding part;

[0009] The microfluidic chip further comprises a broken piece detection line arranged in the non-detection area, both ends of the broken piece detection line are electrically connected to the binding part, and the broken piece detection line extends from the first end to the second end around the detection area.

[0010] The impedance value between the first end and the second end is detected by the binding part, or a detection signal is input at the first end by the binding part, and a feedback signal is received at the second end, so as to detect whether the microfluidic chip is broken.

[0011] The two ends of the broken piece detection line are respectively electrically connected to the opposite ends of the binding part; the binding part comprises a plurality of vacant pins, and the first end and the second end are respectively electrically connected to different vacant pins.

[0012] The microfluidic chip further comprises a first conductive part and a second conductive part arranged in the non-detection area and located at opposite sides of the detection area and close to the binding part, and the first conductive part and the second conductive part are electrically connected to the binding part for transmitting a common voltage.

[0013] The first conductive part and the second conductive part comprise conductive silver glue.

[0014] The microfluidic chip further comprises a common electrode trace arranged in the non-detection area, the common electrode trace surrounds the detection area, and the common electrode trace is electrically connected to the first conductive part and the second conductive part.

[0015] The detection area comprises a plurality of driving electrodes for driving droplets.

[0016] The microfluidic chip further comprises an electrode detection line, one end of the electrode detection line is electrically connected to the driving electrode, and the other end is electrically connected to a vacant pin of the binding part, so as to detect the voltage of the driving electrode.

[0017] To solve the above technical problems, the second technical solution provided by the present application is to provide a microfluidic device. The microfluidic device comprises:

[0018] The microfluidic chip is as described in the above technical solution.

[0019] The control module is electrically connected to the binding part of the microfluidic chip, and is used to provide a driving signal to the microfluidic chip and detect whether the microfluidic chip is damaged.

[0020] The control module comprises a driving chip, and the driving chip comprises a signal sending port and a signal receiving port.

[0021] The signal sending port is electrically connected to the first end of the broken piece detection line through the binding part, so as to send a detection signal to the first end of the broken piece detection line; the detection signal comprises a square wave, a triangular wave, or a sine wave.

[0022] The signal receiving port is electrically connected to the second end of the broken piece detection line through the binding part, so as to receive a feedback signal of the second end of the broken piece detection line.

[0023] The driving chip is used to judge whether the waveform of the feedback signal is the same as the waveform of the detection signal, and in response to the waveform of the feedback signal being different from the waveform of the detection signal, an alarm signal is outputted, and the alarm signal is used to represent that the driving chip is damaged.

[0024] The control module includes a first detection port and a second detection port, the first detection port is electrically connected with the first end of the fragment detection line through the binding part, and the second detection port is electrically connected with the second end of the fragment detection line through the binding part.

[0025] The impedance between the first detection port and the second detection port is detected to determine whether the microfluidic chip is damaged according to the impedance value.

[0026] The detection area of the microfluidic chip includes a plurality of detection units, each detection unit includes an electrode driving circuit and a driving electrode electrically connected to the electrode driving circuit for driving droplets, and the microfluidic chip further includes an electrode detection line, one end of the electrode detection line is electrically connected to the driving electrode, and the other end is electrically connected to the idle pin of the binding part for detecting the potential of the driving electrode.

[0027] The control module includes a driving chip, the driving chip includes a voltage collection port, the voltage collection port is electrically connected with the electrode detection line through the binding part, and is used for receiving the voltage of the driving electrode.

[0028] The driving chip is used for providing scanning signals and data signals of different gears to the electrode driving circuit, receiving the voltage of the driving electrode under different gears at the voltage collection port, and feeding back the received voltage to a visual interface, so as to adjust the gears of the scanning signals and the data signals according to the droplet driving condition and the received voltage.

[0029] The detection area of the microfluidic chip includes a plurality of detection units, each detection unit includes an electrode driving circuit and a driving electrode electrically connected to the electrode driving circuit for driving droplets, and the microfluidic chip further includes an electrode detection line, one end of the electrode detection line is electrically connected to the driving electrode, and the other end is electrically connected to the idle pin of the binding part for detecting the potential of the driving electrode.

[0030] The control module includes a voltage detection port and a driving chip, the driving chip is used for providing scanning signals and data signals of different gears to the electrode driving circuit; the voltage of the driving electrode under different gears is received at the voltage detection port, so as to adjust the gears of the scanning signals and the data signals according to the droplet driving condition and the received voltage.

[0031] The beneficial effects of the present application: Different from the prior art, the present application provides a microfluidic chip and a microfluidic device. The microfluidic chip is provided with a broken piece detection line in a non-detection area. The two ends of the broken piece detection line are electrically connected to the binding part, and the broken piece detection line extends from the first end to the second end around the detection area. That is, the broken piece detection line is around the periphery of the substrate. When the microfluidic chip is damaged, the broken piece detection line can be broken. Therefore, the impedance value between the first end and the second end of the broken piece detection line can be detected by the binding part to determine whether the microfluidic chip is damaged. That is, if it is damaged, the detected resistance value is infinite. Alternatively, a detection signal can be input at the first end of the broken piece detection line through the binding part, and a feedback signal is received at the second end. By judging the case of the feedback signal received at the second end, it can be determined whether the microfluidic chip is damaged. That is, if it is damaged, no feedback signal is received at the second end, or the received feedback signal is abnormal. By detecting whether the microfluidic chip is damaged before experimental detection, the biochemical reagent can be effectively prevented from being used invalidly, and the cost of biochemical detection can be effectively avoided. BRIEF DESCRIPTION OF DRAWINGS

[0032] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without any creative labor.

[0033] Figure 1 is a structural schematic diagram of a first embodiment of the microfluidic chip provided by the present application;

[0034] Figure 2 is a structural schematic diagram of a microfluidic device provided by the first embodiment of the present application;

[0035] Figure 3 is a waveform diagram of a detection signal and a feedback signal provided by an embodiment of the present application;

[0036] Figure 4 is a structural schematic diagram of a microfluidic device provided by a second embodiment of the present application;

[0037] Figure 5 is a structural schematic diagram of a second embodiment of the microfluidic chip provided by the present application;

[0038] Figure 6 is a structural schematic diagram of a microfluidic device provided by a third embodiment of the present application;

[0039] Figure 7 is a structural schematic diagram of a third embodiment of the microfluidic chip provided by the present application;

[0040] Figure 8 is a structural schematic diagram of a microfluidic device provided by a fourth embodiment of the present application;

[0041] Figure 9 is a structural schematic diagram of a microfluidic device provided by a fifth embodiment of the present application.

[0042] Reference signs:

[0043] 100, microfluidic chip; 10, substrate; 11, detection area; 111, driving electrode; 12, grounding line; 121, first part; 122, second part; 13, driving power supply trace; 131, body part; 132, extension part; 14, broken piece detection line; 141, first end; 142, second end; 151, first conductive part; 152, second conductive part; 16, common electrode trace; 17, electrode detection line; 18, non-detection area; 20, binding part; 30, driving chip; 31, signal sending port; 32, signal receiving port; 33, voltage collection port; 41, first detection port; 42, second detection port; 43, voltage detection port; 200, control module; FPC, flexible circuit board. DETAILED DESCRIPTION

[0044] The scheme of the embodiments of the present application will be described in detail below with reference to the accompanying drawings of the specification.

[0045] In the following description, specific details are set forth in order to provide a thorough understanding of the present application. The present application can be practiced without resorting to the details specifically set forth.

[0046] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the accompanying drawings of the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0047] The terms "first", "second", "third", etc. in the present application are only for descriptive purpose, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second", "third" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise explicitly specified. All directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present application are only used to explain the relative position relationship, movement condition, etc. between components in a certain posture (as shown in the drawings), and if the certain posture changes, the directional indications also change accordingly. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device including a series of steps or units is not limited to the listed steps or units, but can optionally include steps or units not listed, or can optionally include other steps or units inherent to the process, method, product or device.

[0048] Reference herein to "embodiment" means that the particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearance of the phrase in various places in the specification indicates that the described feature, structure, or characteristic can be included in at least one embodiment of the application. It is explicitly and implicitly appreciated by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0049] The present application will be described in detail below with reference to the accompanying drawings and embodiments.

[0050] Please refer to Figure 1 , Figure 1 is a structural schematic diagram of a first embodiment of a microfluidic chip provided by the present application. In the present embodiment, a microfluidic chip 100 is provided, which includes a substrate 10, a ground trace and a driving power supply trace 13 arranged on one side of the substrate 10, and a binding portion 20.

[0051] The substrate 10 includes a detection area 11 and a non-detection area 18, and the non-detection area 18 is arranged around the detection area 11, i.e. the central area of the substrate 10 is the detection area 11, which is used for biochemical detection, and the outer periphery of the detection area 11 is the non-detection area 18. Specifically, the substrate 10 includes a transparent glass substrate, which is beneficial for light transmission, so that the microfluidic chip 100 can support photochemical immunoassay and other biochemical detection requiring optical principles.

[0052] The binding part 20 is arranged in the non-detection area 18 and located at the outermost side of the substrate 10, and is used to connect signals outside the chip into the chip, such as working voltage signals, ground signals, common voltage signals, scanning signals, data signals and the like, so as to drive the droplets in the detection area 11 to perform corresponding biochemical detection. The number of the binding part 20 can be one, two, three, four or more, which can be arranged according to the signal interface design and the chip structure layout and the like. In the embodiments of the present application, one binding part 20 is taken as an example for description.

[0053] The ground trace and the driving power supply trace 13 are arranged in the non-detection area 18 and surround the detection area 11. Specifically, the ground trace includes a first part 121 and a second part 122. The two ends of the first part 121 are electrically connected to the opposite ends of the binding part 20 respectively, and the ground trace extends from one end along the non-detection area 18 to the other end around the detection area 11, specifically in the shape of a “H”. The second part 122 is arranged in the “H” and is in the shape of an “L”, and the two ends thereof are connected to the first part 121 respectively, forming a ring with the first part 121. The ring surrounds the outer periphery of the detection area 11, so that the ground signal line of the detection area 11 can be electrically connected to the ground trace at any position, which is beneficial to the layout of the traces of the detection area 11 and can reduce the length of the traces and further reduce the load voltage drop caused by the traces. Specifically, the ground trace can also be divided into multiple parts, and different parts can be arranged on different metal layers. The parts on different metal layers can be electrically connected through vias, which can further optimize the layout of the traces of the detection area 11.

[0054] The driving power supply trace 13 is used to transmit working voltage signals, which are usually direct current constant voltage signals. The driving power supply trace 13 includes a body part 131 and a connecting part. The body part 131 surrounds the outer periphery of the detection area 11 and is in the shape of a ring. The connecting part connects the body part 131 and the binding part 20, so that the external working voltage signals enter the extension part 132 of the driving power supply trace 13 through the binding part 20 and are transmitted to the body part 131 through the extension part 132, so that the driving power supply signal line of the detection area 11 can be electrically connected to the driving power supply trace 13 at any position, so as to introduce the working voltage signals into the detection area 11, which is beneficial to the layout of the traces of the detection area 11 and can reduce the length of the traces and further reduce the load voltage drop caused by the traces. Specifically, the body part 131 and the connecting part of the driving power supply trace 13 can also include multiple parts, and different parts can be arranged on different metal layers. The parts on different metal layers can be electrically connected through vias, which can further optimize the layout of the traces of the detection area 11.

[0055] Furthermore, the microfluidic chip 100 further includes a fragment detection line 14 disposed in the non-detection area 18. Both ends of the fragment detection line 14 are electrically connected to the bonding part 20 respectively, and the fragment detection line 14 extends from the first end 141 around the detection area 11 to the second end 142. Specifically, the fragment detection line 14 may be in a "冂" shape or other curved shapes, as long as it extends from the first end 141 to the second end 142 in a non-closed figure and surrounds the outside of the substrate 10, and its first end 141 and second end 142 are electrically connected to the opposite ends of the bonding part 20 respectively. Through the above settings, the fragment detection line 14 surrounds the periphery of the substrate 10, and the fragment detection line 14 can be broken along with the breakage of the microfluidic chip 100. Furthermore, by detecting the impedance value between the first end 141 and the second end 142 through the bonding part 20, or by inputting a detection signal at the first end 141 through the bonding part 20 and receiving a feedback signal at the second end 142, it is used to detect whether the microfluidic chip 100 is broken.

[0056] Specifically, the first end 141 and the second end 142 can be led out to an external detection point through the bonding part 20 and an external connecting wire, and a multimeter or other instrument that can detect the resistance value is used to connect to the detection point to detect the resistance value of the fragment trace. If the microfluidic chip 100 is not broken, the fragment detection line 14 is a complete and conductive trace, and the impedance between the first end 141 and the second end 142 of the fragment detection line 14 is a fixed value; if the microfluidic chip 100 is broken, the fragment detection line 14 is broken accordingly, and the detected resistance value is infinite; thus, it can be detected whether the microfluidic chip 100 is broken through the fragment detection line 14. By performing the above detection on the microfluidic chip 100 before the biochemical experiment detection, it can effectively prevent the biochemical reagent from being used ineffectively and can effectively avoid the increase in the cost of biochemical detection.

[0057] Or, a detection signal can be input at the first end 141 through the bonding part 20, and a feedback signal can be received at the second end 142 through the bonding part 20. If the microfluidic chip 100 is not broken, the fragment detection line 14 is a complete and conductive trace, then a feedback signal can be received at the second end 142 through the bonding part 20, and the waveform of the feedback signal is the same as the waveform of the detection signal input at the first end 141; if the microfluidic chip 100 is broken, the fragment detection line 14 is broken accordingly, and the fragment detection line 14 cannot be conducted, so no feedback signal is received at the second end 142 or the received feedback signal is an abnormal signal; thus, it can be detected whether the microfluidic chip 100 is broken through the fragment detection line 14. By performing the above detection on the microfluidic chip 100 before the biochemical experiment detection, it can effectively prevent the biochemical reagent from being used ineffectively and can effectively avoid the increase in the cost of biochemical detection.

[0058] In specific embodiments, the two ends of the breakage detection line 14 are respectively electrically connected to the opposite ends of the binding portion 20; the binding portion 20 includes a plurality of vacant pins, the first end 141 and the second end 142 are respectively electrically connected to different vacant pins, by connecting the first end 141 and the second end 142 to different vacant pins, the breakage detection of the microfluidic chip 100 is realized by the redundant design of the binding portion 20, without the need to additionally design the pins of the binding portion 20.

[0059] Please refer to Figure 2 , Figure 2 is a structural schematic diagram of a microfluidic device provided by the first embodiment of the present application. In the present embodiment, a microfluidic device is provided, which includes a microfluidic chip 100 and a control module 200 as involved in the above embodiments. The control module 200 is electrically connected to the binding portion 20 of the microfluidic chip 100, for providing a driving signal to the microfluidic chip 100, and detecting whether the microfluidic chip 100 is broken.

[0060] Specifically, the microfluidic chip 100 is the microfluidic chip 100 provided in the above embodiments. The control module 200 can be bound to the binding portion 20 of the microfluidic chip 100 through a flexible circuit board FPC (Flexible Printed Circuit, FPC), and the control module 200 can be a PCB fixture board. The control module 200 includes a driving chip 30, which is electrically connected to the microfluidic chip 100 through a flexible circuit board FPC, for providing a driving signal to the microfluidic chip 100.

[0061] In the present embodiment, the driving chip 30 has a signal output and feedback function, and the driving chip 30 includes a signal sending port 31 and a signal receiving port 32. The signal sending port 31 is electrically connected to the pin of the first end 141 of the breakage detection line 14 through the flexible circuit board FPC and the binding portion 20, and the signal receiving port 32 is electrically connected to the pin of the second end 142 of the breakage detection line 14 through the flexible circuit board FPC and the binding portion 20.

[0062] Before biochemical detection experiment is carried out, the microfluidic chip 100 is first broken piece detection. Specifically, the driving chip 30 outputs a detection signal at the signal sending port 31, so that the detection signal is transmitted to the first end 141 of the broken piece detection line 14 through the flexible circuit board FPC and the binding part 20; if the microfluidic chip 100 is not damaged, the detection signal can be transmitted along the broken piece detection line 14 to the second end 142, and then transmitted from the second end 142 to the driving chip 30 through the binding part 20 and the flexible circuit board FPC, so that the driving chip 30 receives the feedback signal at the signal receiving port 32; if the microfluidic chip 100 is damaged, since the broken piece detection line 14 is broken, the detection signal cannot be normally transmitted along the broken piece detection line 14, so that the driving chip 30 cannot receive the feedback signal at the signal receiving port 32, or the feedback signal received is an abnormal signal. Further, the driving chip 30 can feed back the detection result to the visual terminal in response to the above detection result, and output an alarm signal to the visual terminal in response to the waveform of the feedback signal being different from the waveform of the detection signal; or, the control module 200 further includes an alarm unit electrically connected with the driving chip 30, and the driving chip 30 outputs an alarm signal to the alarm unit in response to the above detection result, so as to remind the user that the microfluidic chip 100 is damaged and needs to be replaced. The alarm signal is used to indicate that the driving chip 30 is damaged.

[0063] Please refer to Figure 3 , Figure 3 is a waveform diagram of the detection signal and the feedback signal provided by an embodiment of the present application. Specifically, the detection signal includes a square wave, a triangular wave, a sine wave, a step wave or other waveforms, which can be set according to actual needs. In this embodiment, the detection signal is a square wave, wherein the driving chip 30 outputs a square wave signal at the sending port, and if the microfluidic chip 100 is not damaged, the feedback signal received by the driving chip 30 at the signal receiving port 32 is also a square wave signal; if the microfluidic chip 100 is damaged, the driving chip 30 does not receive the feedback signal at the signal receiving end.

[0064] Please refer to Figure 4 , Figure 4 is a structural diagram of a microfluidic device provided by a second embodiment of the present application. In this embodiment, the control module 200 includes a first detection port 41 and a second detection port 42, the first detection port 41 is electrically connected with the first end 141 of the broken piece detection line 14 through the flexible circuit board FPC and the binding part 20, and the second detection port 42 is electrically connected with the second end 142 of the broken piece detection line 14 through the flexible circuit board FPC and the binding part 20.

[0065] Before the biochemical detection experiment, the microfluidic chip 100 is first detected for breakage. Specifically, when the breakage detection is performed, a multimeter or other instrument capable of detecting resistance value is used to connect the first detection port 41 and the second detection port 42 to detect the resistance value of the breakage trace. If the microfluidic chip 100 is not damaged, the breakage detection line 14 is a complete and conductive trace, and the impedance between the first end 141 and the second end 142 of the breakage detection line 14 is a fixed value; if the microfluidic chip 100 is damaged, the breakage detection line 14 is broken, and the detected resistance value is infinite; thus, whether the microfluidic chip 100 is damaged can be detected through the breakage detection line 14. By performing the above detection on the microfluidic chip 100 before the biochemical experiment, the biochemical reagent can be effectively prevented from being used invalidly, and the cost of biochemical detection can be effectively avoided from increasing.

[0066] Referring to Figure 5 , Figure 5 is a structural schematic diagram of a second embodiment of the microfluidic chip provided in the present application. In the present embodiment, in addition to the above structure, the microfluidic chip 100 further comprises a first conductive part 151 and a second conductive part 152 arranged in the non-detection area 18, which are respectively located on the opposite sides of the detection area 11 and close to the binding part 20. The first conductive part 151 and the second conductive part 152 are respectively electrically connected to the binding part 20, and are used to transmit a common voltage.

[0067] Specifically, the first conductive part 151 and the second conductive part 152 comprise conductive silver paste; generally, the common electrode of the counter substrate arranged above the microfluidic chip 100 is electrically connected to the first conductive part 151 and the second conductive part 152 on the microfluidic chip 100 through a via hole, so that the common voltage signal is transmitted to the counter substrate through the first conductive part 151 and the second conductive part 152.

[0068] Further, the microfluidic chip 100 further comprises a common electrode trace 16 arranged in the non-detection area 18, which surrounds the detection area 11, and the common electrode trace 16 is respectively electrically connected to the first conductive part 151 and the second conductive part 152. That is, the common electrode trace 16 is introduced on the periphery of the substrate 10 to realize the communication of the common electrode in the plane of the microfluidic chip 100, that is, the first conductive part 151 and the second conductive part 152 are conducted by introducing the common electrode trace 16, so as to prevent the problem that the common voltage signals are different due to different pins of the flexible circuit board FPC giving the common voltage signals to the first conductive part 151 and the second conductive part 152, or the problem that the common voltage signal level is uneven due to the unilateral conduction caused by the pressing of the flexible circuit board FPC pin and the binding part 20.

[0069] Referring to Figure 6 , Figure 6The microfluidic device is provided by the third embodiment of the present application. The microfluidic device comprises a microfluidic chip 100 and a control module 200, the control module 200 is electrically connected to the binding part 20 of the microfluidic chip 100, used to provide a driving signal to the microfluidic chip 100, and detect whether the microfluidic chip 100 is damaged.

[0070] Specifically, the microfluidic chip 100 is the microfluidic chip 100 provided by the above-mentioned Figure 5 embodiment, and the same technical effects can be achieved. The control module 200 is bound to the binding part 20 of the microfluidic chip 100 through the flexible circuit board FPC, and the structure and function of the control module 200 are the same as those of the above-mentioned Figure 4 or Figure 5 control module 200, and the same technical effects can be achieved. For details, please refer to the above detailed introduction, which will not be repeated here.

[0071] Please refer to Figure 7 , Figure 7 The structure diagram of the third embodiment of the microfluidic chip provided by the present application is shown. In this embodiment, in addition to the structure in the above-mentioned embodiments, the detection area 11 of the microfluidic chip 100 comprises a plurality of detection units, each detection unit comprises an electrode driving circuit and a driving electrode 111 electrically connected to the electrode driving circuit, used to drive the droplet; the microfluidic chip 100 further comprises an electrode detection line 17, one end of the electrode detection line 17 is electrically connected to the driving electrode 111, and the other end is electrically connected to the idle pin of the binding part 20, so as to detect the potential of the driving electrode 111, realize real-time monitoring of the driving electrode 111, and provide basis for digital microfluidic device to adjust data voltage.

[0072] Specifically, the design of the driving unit of the microfluidic chip 100 focuses on two processes: first, the charging process, to ensure that the charging voltage reaches the specified data voltage and working voltage level; second, the discharging process (reset process), the data line input low reference potential, to realize the driving electrode 111 voltage level as low reference potential.

[0073] In this embodiment, by setting the electrode detection line 17, and electrically connecting one end of the electrode detection line 17 to the driving electrode 111, and electrically connecting the other end to the idle pin of the binding part 20, the voltage monitoring of the driving electrode 111 can be realized through the software interface without damaging the microfluidic chip 100, or through the simple oscilloscope measurement, so as to facilitate the inspection of the relationship between the droplet driving and the input data voltage level, thereby improving the working efficiency. For details of the detection method, please refer to the detailed description below.

[0074] Please refer to Figure 8 , Figure 8is a structural schematic diagram of a microfluidic device provided in a fourth embodiment of the present application. In the embodiment, the microfluidic device comprises a microfluidic chip 100 and a control module 200 electrically connected to the microfluidic chip 100. The microfluidic chip 100 is the microfluidic chip 100 in the above Figure 7 The control module 200 comprises a driving chip 30, and the driving chip 30 comprises a voltage collection port 33 electrically connected to the electrode detection line 17 through the binding part 20, and is configured to receive the voltage of the driving electrode 111.

[0075] Specifically, the driving chip 30 is configured to provide the scanning signal and the data signal of different gears to the electrode driving circuit, receive the voltage of the driving electrode 111 under different gears at the voltage collection port 33, and feed back the received voltage to a visual interface; so as to adjust the gears of the scanning signal and the data signal according to the droplet driving condition and the received voltage.

[0076] In the present application, the driving chip 30 has a signal receiving function and feeds back to the software interface display. As shown in the figure, the voltage of the driving electrode 111 at a certain position in the detection area 11 is led out through the electrode detection line 17, and enters the driving chip 30 through the flexible circuit board FPC. Specifically, the control module 200 can transmit the scanning signal and the data signal of different gears to the driving unit of the microfluidic chip 100 respectively, so as to receive the voltage of the driving electrode 111 under different gears at the voltage collection port 33, timely capture the voltage of the driving electrode 111 in the detection area 11, and feed back the received voltage to the visual interface, so as to determine whether the gear needs to be adjusted according to the droplet driving condition.

[0077] For example, the three gears can include a first gear, a second gear and a third gear. The first gear is a low gear, in which the voltage of the scanning signal can be 40V, and the voltage of the data voltage signal can be 35V. The second gear is a middle gear, in which the voltage of the scanning signal can be 45V, and the voltage of the data voltage signal can be 40V. The third gear is a high gear, in which the voltage of the scanning signal can be 50V, and the voltage of the data voltage signal can be 45V.

[0078] Please refer to Figure 9 , Figure 9 is a structural schematic diagram of a microfluidic device provided in a fifth embodiment of the present application. In the embodiment, the microfluidic device comprises a microfluidic chip 100 and a control module 200 electrically connected to the microfluidic chip 100. The microfluidic chip 100 is the microfluidic chip 100 in the above Figure 7 The control module 200 comprises a driving chip 30, and the driving chip 30 comprises a voltage collection port 33 electrically connected to the electrode detection line 17 through the binding part 20, and is configured to receive the voltage of the driving electrode 111.

[0079] Specifically, the control module 200 includes a voltage detection port 43 and a driving chip 30, the driving chip 30 is used to provide the scanning signal and the data signal of different gears to the electrode driving circuit; the voltage of the driving electrode 111 under different gears is received through the voltage detection port 43, so as to adjust the gear of the scanning signal and the data signal according to the droplet driving condition and the received voltage.

[0080] In the embodiment, if the driving chip 30 does not have the signal receiving function and feedback to the software interface display. As shown in the figure, the voltage of the driving electrode 111 at a certain position in the detection area 11 is led out through the electrode detection line 17, enters the voltage detection port 43 of the control module 200 through the flexible circuit board FPC, the size of the voltage detection port 43 is visible to the eye, the control module 200 transmits the driving signal of different gears to the driving unit of the microfluidic chip 100, then the voltage of the driving electrode 111 is collected through the signal measurement instrument, for example, the oscilloscope is connected with the voltage detection port 43 to collect the voltage of the driving electrode 111, then according to the droplet driving condition, it can be determined whether the gear needs to be adjusted. Specifically, the gear can include the gear in the above embodiment, or, according to the circuit design of the microfluidic, the voltage value of different gears can be set, and more gears can also be included.

[0081] The above is only the embodiment of the present application, and does not limit the patent protection scope of the present application, any equivalent structure or equivalent process transformation using the content of the specification and drawings of the present application, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A microfluidic chip, comprising: a substrate comprising a detection region and a non-detection region surrounding the detection region; a ground trace and a driving power supply trace disposed in the non-detection region and surrounding the detection region, respectively; a binding portion disposed in the non-detection region, the ground trace and the driving power supply trace being electrically connected to the binding portion; characterized in that the microfluidic chip further comprises a chip breakage detection line disposed in the non-detection region, two ends of the chip breakage detection line being electrically connected to the binding portion, respectively, and the chip breakage detection line extending from a first end to a second end around the detection region; the two ends of the chip breakage detection line being electrically connected to opposite ends of the binding portion, respectively; the binding portion comprising a plurality of vacant pins, the first end and the second end being electrically connected to different vacant pins, respectively; impedance between the first end and the second end is detected through the binding portion, or a detection signal is input at the first end through the binding portion, and a feedback signal is received at the second end for detecting whether the microfluidic chip is broken; the detection region comprising a plurality of driving electrodes for driving droplets; the microfluidic chip further comprising an electrode detection line, one end of the electrode detection line being electrically connected to the driving electrodes, and the other end being electrically connected to a vacant pin of the binding portion for detecting voltage of the driving electrodes.

2. The microfluidic chip of claim 1, wherein, the microfluidic chip further comprising a first conductive portion and a second conductive portion disposed in the non-detection region, respectively, located on opposite sides of the detection region and close to the binding portion, the first conductive portion and the second conductive portion being electrically connected to the binding portion, respectively, for transmitting a common voltage.

3. The microfluidic chip of claim 2, wherein, the first conductive portion and the second conductive portion comprising conductive silver paste; the microfluidic chip further comprising a common electrode trace disposed in the non-detection region, the common electrode trace surrounding the detection region, and the common electrode trace being electrically connected to the first conductive portion and the second conductive portion, respectively.

4. A microfluidic device, characterized in that, comprising: a microfluidic chip as claimed in any one of claims 1-3; a control module electrically connected to the binding portion of the microfluidic chip for providing a driving signal to the microfluidic chip and detecting whether the microfluidic chip is broken.

5. The microfluidic device of claim 4, wherein, the control module comprising a driving chip, the driving chip comprising a signal sending port and a signal receiving port; wherein, the signal sending port being electrically connected to the first end of the chip breakage detection line through the binding portion for sending a detection signal to the first end of the chip breakage detection line; the detection signal comprising a square wave, a triangular wave, or a sine wave; the signal receiving port being electrically connected to the second end of the chip breakage detection line through the binding portion for receiving a feedback signal at the second end of the chip breakage detection line; the driving chip being configured to judge whether a waveform of the feedback signal is the same as a waveform of the detection signal, and in response to the waveform of the feedback signal being different from the waveform of the detection signal, output a warning signal, the warning signal being used to represent that the driving chip is broken.

6. The microfluidic device of claim 4, wherein, The control module comprises a first detection port and a second detection port, the first detection port is electrically connected with the first end of the fragment detection line through the binding part, and the second detection port is electrically connected with the second end of the fragment detection line through the binding part; The impedance between the first detection port and the second detection port is detected to determine whether the microfluidic chip is damaged according to the impedance value.

7. The microfluidic device of claim 4, wherein, The detection area of the microfluidic chip comprises a plurality of detection units, each detection unit comprises an electrode driving circuit and a driving electrode electrically connected with the electrode driving circuit for driving droplets; the microfluidic chip further comprises an electrode detection line, one end of the electrode detection line is electrically connected to the driving electrode, and the other end is electrically connected to the idle pin of the binding part for detecting the potential of the driving electrode. The control module comprises a driving chip, the driving chip comprises a voltage collection port, the voltage collection port is electrically connected with the electrode detection line through the binding part, and is used for receiving the voltage of the driving electrode; The driving chip is used for providing scanning signals and data signals of different gears to the electrode driving circuit, receiving the voltage of the driving electrode under different gears at the voltage collection port, and feeding back the received voltage to a visual interface; The gear of the scanning signal and the data signal is adjusted according to the driving condition of the droplet and the received voltage.

8. The microfluidic device of claim 4, wherein, The detection area of the microfluidic chip comprises a plurality of detection units, each detection unit comprises an electrode driving circuit and a driving electrode electrically connected with the electrode driving circuit for driving droplets; the microfluidic chip further comprises an electrode detection line, one end of the electrode detection line is electrically connected to the driving electrode, and the other end is electrically connected to the idle pin of the binding part for detecting the potential of the driving electrode. The control module comprises a voltage detection port and a driving chip, the driving chip is used for providing scanning signals and data signals of different gears to the electrode driving circuit; the voltage of the driving electrode under different gears is received at the voltage detection port, so as to adjust the gear of the scanning signal and the data signal according to the driving condition of the droplet and the received voltage.

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