A test method for cosmetic repair effect and its application

Abstract

The present invention provides a test method for the repair effect of cosmetics and its application, the test method comprising the steps of establishing a skin model and testing the effect of a sample. In the present invention, a skin model is quickly established by selecting the inner skin of the forearm of a subject's arm, thereby avoiding the direct use of facial skin for long-term testing, improving safety, making the subject's acceptance degree high, and greatly reducing the test cost. In addition, the modeling success rate is high and the test result reliability is high. When the sample repair effect test is performed, the test result can be measured in 1-2 hours, which greatly shortens the test cycle and reduces the test cost. The test method is used to test the repair effect of cosmetics, which can help R&D personnel to develop better products more quickly and efficiently.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetic testing, and in particular to a method for testing the repairing effect of cosmetics and an application thereof. Background Art

[0002] With the rapid development of the cosmetics industry, consumers have a growing demand for cosmetics, and their requirements are becoming higher and higher. The purpose of cosmetics efficacy testing is to evaluate the actual effects and safety of cosmetics to ensure the rights and health of consumers. The efficacy of cosmetics includes moisturizing, whitening, repairing, etc., and the actual efficacy of the product can be evaluated through scientific testing methods.

[0003] For the repair effect of cosmetics, the commonly used test method is usually the human skin test of the subjects. Generally, subjects with skin problems are selected to use samples, and in order to ensure that the repair and other effects of the product can be accurately evaluated, the use cycle of the sample needs to be maintained at least 28 days, which leads to a long repair effect test cycle, slow detection speed, and high detection cost. During the 28-day use process, the subjects cannot be supervised, and the test results are inaccurate due to the use of the subjects. At the same time, the existing cosmetic effect test is usually conducted on the facial skin of the subject. Due to the uncertainty of the effect of the cosmetics to be tested, especially for some subjects with sensitive skin, direct use of facial skin for testing has potential risks such as easy allergies and easy damage to the skin. It makes it more difficult to recruit subjects, high R&D costs and no guarantee of safety.

[0004] Therefore, how to test the repair effect of cosmetics quickly and accurately without causing harm to the subjects is the current research focus in the field of cosmetics testing. Summary of the invention

[0005] In view of the above problems, the object of the present invention is to provide a method for testing the repair effect of cosmetics which is efficient, accurate and safe for test subjects.

[0006] In one aspect, the present invention provides a method for testing the repair effect of cosmetics, and the specific testing steps of the testing method are as follows:

[0007] Step S1: Establishing a skin model: setting a sample area and a control area on the skin on the inner side of the subject's arm, and then using tape to tear the sample area and the control area on the inner side of the arm to establish a skin model for testing; and testing the transepidermal water loss rate of the skin;

[0008] Step S2: Perform a repair effect test: apply the cosmetic sample to be tested to the sample area on the inner side of the arm, and then test the transepidermal water loss rate of the sample area and the control area on the inner side of the arm to obtain the repair effect of the cosmetic to be tested.

[0009] In order to better ensure the safety of the subjects and the reliability of the experimental results in the present invention, preferably, the skin model is established by selecting the inner skin of the forearm of the subject. This can avoid directly using the facial skin for testing, reducing the potential risks of skin allergies and skin damage of the subject. The subject's acceptance is high, and it is easier to carry out the test, which greatly reduces the cost of the test.

[0010] More preferably, in step S1, before the tearing treatment, the subject's arm is exposed and rested in a constant temperature and humidity chamber for 10-30 minutes. All subjects are placed in the same environment to maintain the consistency of the subject's skin condition, improve the success rate of model establishment and the accuracy of test results.

[0011] The subjects of the present invention are mainly people in the Pearl River Delta region. The ultraviolet rays in the Pearl River Delta region are strong, and the skin barrier is generally in a damaged state. The transepidermal water loss rate of the subject group is mostly 10-15g / m 2 / h range.

[0012] The skin model in step S1 is mainly established by using a tape tearing process. The specific method for verifying the reliability of the skin model is not limited in the present invention. Preferably, the specific steps of the reliability verification are as follows: the skin model of the present invention and the commonly used 28-day test method are used to test the repair effect of the same test sample. If the repair effects obtained by the test are similar, the skin model of the present invention is judged to be reliable.

[0013] Preferably, the specific steps of the tearing treatment in step S1 are: first stick the tape on the sample area and the control area of ​​the skin, press and then stick it for 15-30s to stabilize the pressure, and then tear it off; repeat the tape sticking-tearing steps 6-10 times to obtain a skin model for testing.

[0014] Preferably, the viscosity of the tape is 3-5N. The tape used in the present invention is not specifically limited. Preferably, the tape is 3M transparent tape, medical transparent tape, or Deli transparent tape. The viscosity of the tape will directly affect the tearing effect. Too high a viscosity will not be easy to tear and may cause skin damage to the subject. Therefore, it is necessary to control the viscosity of the tape and cooperate with specific tearing rates and other conditions to achieve successful modeling.

[0015] Preferably, the tearing rate of the tape is 20-40 cm / s. The tearing speed of the tape will affect the success of the skin barrier damage modeling. Too slow tearing speed will easily lead to unsuccessful modeling, while too fast tearing speed will easily lead to excessive skin damage and dermatitis.

[0016] Preferably, the width of the tape is 4-5 cm. The width of the tape affects the uniformity of modeling. Using a tape that is too narrow than 4 cm is prone to incomplete coverage of the test area. Using a tape that is wider than 5 cm is more likely to cause excessive damage to the skin when tearing due to the large coverage area, thereby causing adverse reactions such as dermatitis and papules.

[0017] The tape tearing treatment of the present invention achieves a balance between the effects of various parameters by controlling the pressing time, tearing times, and viscosity of the tape, and can effectively establish a skin test model. The skin state and reaction obtained are most similar to the facial skin state, and after testing, the effect test results are highly accurate. In addition, by controlling the processing conditions, the modeling success rate is high, and it is possible to avoid damage to the skin of the test subject, which would affect the subsequent sample effect test.

[0018] Preferably, the pressing pressure is 20-50N, and the pressing is performed 2-4 times.

[0019] Preferably, the sample area and the control area in step S1 are both 3 cm*3 cm in area, and the interval between each area is at least 1 cm. In the present invention, when setting the test area, in order to further ensure that the position of each area is statistically balanced, the sample area and the control area should be randomly distributed in the test area.

[0020] Preferably, the skin condition of the skin model used for testing in step S1 is: the transepidermal water loss rate of the tested area should be >15g / m 2 / h is considered successful modeling, and when the transepidermal water loss rate of the arm is less than 15g / m 2 / h means modeling failed and tearing modeling should be performed again.

[0021] Preferably, in step S2, after the sample is applied to the sample area, it needs to be retained for 1-2 hours, and then the transepidermal water loss rate of the sample area and the control area of ​​the inner arm skin is tested.

[0022] Another aspect of the present invention provides an application of the above-mentioned cosmetic repair effect testing method in cosmetic repair effect testing, which can be used for repair effect testing of various cosmetics, such as lotions, creams, lotions, etc.

[0023] Beneficial effects:

[0024] (1) The present invention selects the skin on the inner forearm of the subject to establish the skin model, thereby avoiding the direct use of facial skin for long-term testing, improving safety, increasing subject acceptance, and greatly reducing testing costs.

[0025] (2) The present invention adopts the method of tearing the arm to make the subject's skin quickly reach a more ideal test state and quickly establish a skin test model; after using the sample, the test result can be measured in 1-2 hours, which greatly shortens the test cycle and reduces the test cost.

[0026] (3) The present invention has a high modeling success rate by controlling the processing conditions in the establishment of the skin model, and the reliability of the test results is highly reliable after reliability verification. The test method is used to test the repair effect of cosmetics, which can help R&D personnel develop better products more quickly and efficiently. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The skin surface before tearing treatment established by the skin model of Example 3 of the present invention;

[0028] Figure 2 The skin surface after tearing treatment is established for the skin model of Example 3 of the present invention. DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. The following tests are in accordance with the "Cosmetic Safety Technical Specifications 2015 Edition".

[0030] Example

[0031] Thirty subjects aged 18-60 were selected. All the subjects lived in the Pearl River Delta region. After testing, the transepidermal water loss rate of the subjects before the skin modeling tearing treatment was 10-15g / m 2 / h. The test samples used in the embodiments of the present invention are: Kebeier moisturizing repair essence mask, Baifubang hand and foot repair cream, and Meizhuanglian microcrystalline capsule repair mask.

[0032] Example 1

[0033] This embodiment provides a method for testing the repair effect of cosmetics, and the specific steps are as follows:

[0034] Step S1: Establishment of skin model: set a sample area and a control area on the skin of the inner forearm of the subject's arm, the area of ​​the sample area and the control area are both 3cm*3cm, and the interval between each area is 1cm; after the subject's arm skin is exposed in a constant temperature and humidity chamber (temperature is 20℃±1℃, humidity is 50%±10%) for 20 minutes, use tape to tear the skin of the sample area and the control area on the inner side of the arm, stick the tape on the test area, use a roller weighing 80g to roll back and forth on the surface of the tape 3 times, stick it for 30s, stabilize the pressure, and then tear it off, the tearing rate is 30cm / s; repeat the tape-tearing steps 10 times to establish a skin model for testing; use a TEWL transepidermal water loss rate meter (Tewameter) to test the skin test site, and measure the transepidermal water loss rate of the skin;

[0035] The tape used is 3M transparent tape, the tape viscosity is 3N; the tape width is 4.6 cm.

[0036] Step S2: Perform a repair effect test: apply the cosmetic sample to be tested to the sample area on the inner side of the forearm and keep it for 1 hour; test the transepidermal water loss rate of the sample area and the control area on the inner side of the arm when the sample is kept for 1 hour, and obtain the repair effect of the cosmetic to be tested.

[0037] The test sample used in this embodiment is the Cobalt Soothing Repair Essence Mask.

[0038] Example 2

[0039] The present embodiment provides a method for testing the repair effect of cosmetics. The specific implementation method is the same as that of Example 1. The difference from Example 1 is that in step S1, the tearing rate is 30 cm / s, and the tape sticking-tearing steps are repeated 6 times to establish a skin model for testing; a TEWL trans-epidermal water loss meter (Tewameter) is used to test the skin test site to measure the trans-epidermal water loss rate of the skin.

[0040] Example 3

[0041] This embodiment provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of embodiment 1. The difference from embodiment 1 is that in step S1, the sample area and the control area skin on the inner side of the arm are torn with tape, the tape is attached to the test area, and a roller with a weight of 80g is used to roll back and forth on the surface of the tape for 3 times, and the tape is attached for 20s to stabilize the pressure, and then the tape is torn off at a tearing rate of 30cm / s; the tape-attaching-tearing steps are repeated 8 times to establish a skin model for testing; the skin test site is tested using a TEWL transepidermal water loss rate meter (Tewameter) to measure the transepidermal water loss rate of the skin;

[0042] Example 4

[0043] This embodiment provides a method for testing the repair effect of cosmetics. The specific implementation method is the same as that of Embodiment 3. The difference from Embodiment 3 is that in the tearing process in step S1, a roller weighing 60 g is used.

[0044] Example 5

[0045] This embodiment provides a method for testing the repair effect of cosmetics. The specific implementation method is the same as that of Embodiment 3. The difference from Embodiment 3 is that in the tearing process of step S1, the viscosity of the tape is 5N.

[0046] Example 6

[0047] This embodiment provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of Embodiment 3. The difference from Embodiment 3 is that in the tearing process of step S1, the tearing rate is 40 cm / s.

[0048] Example 7

[0049] This embodiment provides a method for testing the repair effect of cosmetics. The specific implementation method is the same as that of Example 3. The difference from Example 3 is that the test sample used in this embodiment is Baifubang Hand and Foot Repair Cream.

[0050] Example 8

[0051] This embodiment provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of Example 3. The difference from Example 3 is that the test sample used in this embodiment is the Meizhuanglian microcrystalline capsule repair mask.

[0052] Comparative Example 1

[0053] This embodiment provides a method for testing the repair effect of cosmetics, and the specific testing steps are:

[0054] Step S1: Establishment of skin model: a sample area and a control area are set on the skin of the inner arm of the subject, the area of ​​the sample area and the control area are both 3 cm*3 cm, and the interval between each area is 1 cm; a TEWL transepidermal water loss rate meter (Tewameter) is used to test the skin test site to measure the transepidermal water loss rate of the skin;

[0055] Step S2: Perform a repair effect test: apply the cosmetic sample to be tested to the sample area on the inner side of the arm and keep it for 28 days; test the transepidermal water loss rate of the sample area and the control area on the inner side of the arm after the sample is kept for 28 days to obtain the repair effect of the cosmetic to be tested.

[0056] The test sample used in this embodiment is the Cobalt Soothing Repair Essence Mask.

[0057] Comparative Example 2

[0058] This comparative example provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of comparative example 1. The difference from comparative example 1 is that the test sample used is Baifubang hand and foot repair cream.

[0059] Comparative Example 3

[0060] This comparative example provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of comparative example 1. The difference from comparative example 1 is that the test sample used is the Meizhuanglian microcrystalline capsule repair mask.

[0061] Comparative Example 4

[0062] This comparative example provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of Example 3. The difference from Example 3 is that in the tearing process of step S1, the viscosity of the tape is 7N.

[0063] Comparative Example 5

[0064] This comparative example provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of Example 3. The difference from Example 3 is that in the tearing process of step S1, the tearing rate of the tape is 50 cm / s.

[0065] Comparative Example 5

[0066] This comparative example provides a test method for the repair effect of cosmetics. The specific implementation method is the same as that of Example 3. The difference from Example 3 is that in the tearing process of step S1, a roller weighing 120 g is used.

[0067] Comparative Example 6

[0068] This comparative example provides a method for testing the repair effect of cosmetics. The specific implementation method is the same as that of Example 3. The difference from Example 3 is that in the tearing process of step S1, the tape-tearing step is repeated 13 times.

[0069] Performance Testing

[0070] 1. Use the TEWL transepidermal water loss meter (Tewameter) to measure the transepidermal water loss rate of the skin.

[0071] The samples were tested using the test methods of Examples 1-8 and Comparative Examples 1-6, and the test results were as shown in Table 1. The test data of the transepidermal water loss rate of 30 subjects in Example 3 are shown in Table 2.

[0072] Table 1

[0073]

[0074] Table 2

[0075]

[0076]

[0077] In the present invention, the initial transepidermal water loss rate of the inner arm skin of the test subject before the skin model is established, the transepidermal water loss rate before the sample test (i.e., the transepidermal water loss rate after the skin model is established), and the transepidermal water loss rate after the sample test are tested respectively.

[0078] Figure 1 and Figure 2 These are the skin surface images of the inner arm skin of a subject in Example 3 before and after the skin model was established. Obviously, after the skin model was established, the subject's skin was slightly red, but there was no swelling or damage, and there was no skin damage to the subject and the impact was minimal. In Example 3, the skin model was established to make the inner arm skin of the subject reach an ideal test state, and then the repair effect of the sample was tested; as can be seen from the data in the above table, in Example 3, after the skin model was established, the reaction of the subject's skin to the test sample was significantly different compared with the sample area and the control area.

[0079] Comparative Example 1 and Example 3, Comparative Example 2 and Example 7, and Comparative Example 3 and Example 8 respectively use the same test samples, and the test methods of Comparative Examples 1-3 are the existing conventional 28-day facial skin test methods. According to the data of Examples 3, 7, and 8 compared with the data of Comparative Examples 1-3, it can be seen that the two achieve similar skin conditions before the sample test, and the transepidermal water loss rates measured after using the samples are also similar, indicating that the test results of the two test methods for the same sample are the same. However, the test methods of Comparative Examples 1-3 have a long cycle, and use the facial skin of the subjects, which has high safety risks.

[0080] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for testing the repair effect of cosmetics, characterized in that: The specific test steps of the test method are as follows: Step S1: Establishing a skin model: setting a sample area and a control area on the skin on the inner side of the subject's arm, and then using tape to tear the sample area and the control area on the inner side of the arm to establish a skin model for testing; and testing the transepidermal water loss rate of the skin; Step S2: Perform a repair effect test: apply the cosmetic sample to be tested to the sample area on the inner side of the arm, and then test the transepidermal water loss rate of the sample area and the control area on the inner side of the arm to obtain the repair effect of the cosmetic to be tested; The specific steps of the tearing treatment in step S1 are: first stick the tape on the sample area and the control area of ​​the skin, press and then stick for 15-30 seconds to stabilize the pressure, and then tear it off; repeat the tape sticking-tearing steps 6-10 times to obtain a skin model for testing; the pressing pressure is 20-50 N, and the pressing is performed 2-4 times; The viscosity of the tape is 3-5N; the tearing rate of the tape is 20-40 cm / s; The width of the tape is 4-5 cm; The sample area and the control area in step S1 are both 3 cm*3 cm in area, and the interval between each area is at least 1 cm; The skin condition of the skin model used for testing in step S1 is: the transepidermal water loss rate of the test area should be >15g / m² / h to be considered as successful modeling, and when the transepidermal water loss rate of the arm is <15g / m² / h, the modeling fails and the tearing modeling should be repeated.

2. A method for testing the repair effect of cosmetics according to claim 1, characterized in that: In step S1, before the tearing treatment, the subjects were asked to keep their arms exposed and rest in a constant temperature and humidity chamber for 10-30 minutes.

3. Application of the test method for the repairing effect of cosmetics according to any one of claims 1 to 2 in the test of the repairing effect of cosmetics.

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