A pesticide composition containing profenofos and its application

Abstract

The present invention belongs to the technical field of pesticide acaricides and discloses a pesticide composition containing profenofos and its application. The active ingredients of the pesticide composition include a compound of formula I: #imgabs0# (Formula I) and profenofos, and the mass ratio of the compound of formula I to profenofos is 1:45 to 50:1. The pesticide composition of the present invention has an excellent synergistic effect on common pest mites of crops, a significant acaricidal effect, can effectively reduce the dosage of the pesticide, and slow down the development of drug resistance of the pest mites.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural acaricide and discloses a pesticide composition containing profenofos and application thereof. Background Art

[0002] Profenofos, commonly known as profenofos and chemically named 4-bromo-2-chloro-1-[ethoxy(propylthio)phosphoryl]oxybenzene or O-ethyl-O-(4-bromo-2-chlorophenyl)-S-propylthiophosphate, is a broad-spectrum organophosphorus insecticide and acaricide. Profenofos is an acetylcholinesterase inhibitor that acts on the pest nervous system. This enzyme terminates the action of the excitatory neurotransmitter acetylcholine in nerve synapses, leading to increased acetylcholine accumulation and continuous activation of receptors on the postsynaptic membrane, causing overexcitement, convulsions, paralysis, and death in the pest.

[0003] The compound of formula I, whose chemical name is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl-3-yl])(2,2,2-trifluoroethyl) sulfide, has excellent control effects on common plant pests and mites. Its structural formula is as follows:

[0004]

[0005] Pest mites include the Tetranychus, Acaridae, and Tenebrionidae families. Among these, the most harmful are the Panonychus, Tetranychus cinnabarinus, and Tetranychus urticae. Pest mites have many generations, strong reproductive capacity, and a high degree of self-fertilization, making them prone to developing resistance to acaricides. Frequent pesticide applications increase agricultural costs and worsen environmental pollution.

[0006] Combining acaricides with different mechanisms of action is a common approach to combating the development of pesticide resistance in pests and controlling resistant mites. Different ingredients are combined, and actual application results determine whether a given compound produces synergistic, additive, or antagonistic effects. Synergistic compounding significantly improves control effectiveness, reduces pesticide dosage, and thus delays the development of resistance in mites, making it an effective comprehensive approach for mite control. Currently, no reports exist on the combination of a compound of Formula I with profenofos, or its application in mite control. Summary of the Invention

[0007] To address the shortcomings of the prior art, the present invention provides a pesticide composition containing profenofos and its use. This pesticide composition, composed of a compound of Formula I and profenofos with different mechanisms of action, exhibits excellent control efficacy against mite pests within an appropriate mass ratio, exhibits a broad spectrum of control, exhibits rapid and sustained efficacy, and exhibits a long duration of action, thereby helping to delay the development of pesticide resistance in mite pests. Another object of the invention is to provide the use of this composition for controlling mite pests in crops such as fruit trees, cotton, and vegetables.

[0008] In order to achieve the above object, the present invention adopts the following technical solution: a pesticide composition containing profenofos, wherein the active ingredient of the pesticide composition comprises a compound of formula I: and profenofos, the mass ratio of the compound of formula I to profenofos is 1:45 to 50:1, such as 1:45, 1:40, 1:30, 1:25, 1:20, 1:15, 1:7, 1:5, 1:3, 1:2, 2:1, 3:1, 5:1, 7:1, 10:1, 15:1, 20:1, 25:1, 30:1, 45:1, 50:1, or any value between the above numerical ranges;

[0009] Furthermore, the mass ratio of the compound of formula I to profenofos is 1:40 to 45:1, such as 1:40, 1:30, 1:25, 1:20, 1:15, 1:7, 1:5, 1:3, 1:2, 2:1, 3:1, 5:1, 7:1, 10:1, 15:1, 20:1, 25:1, 30:1, 45:1 or any value within the above numerical range;

[0010] Furthermore, the mass ratio of the compound of formula I to profenofos is 1:25 to 45:1, such as 1:25, 1:20, 1:15, 1:7, 1:5, 1:3, 1:2, 2:1, 3:1, 5:1, 7:1, 10:1, 15:1, 20:1, 25:1, 30:1, 45:1, or any value between the above numerical ranges;

[0011] Furthermore, the mass ratio of the compound of formula I to profenofos is 1:20 to 10:1, such as 1:20, 1:15, 1:7, 1:5, 1:3, 1:2, 2:1, 3:1, 5:1, 7:1, 10:1 or any value between the above numerical ranges;

[0012] Furthermore, based on 100 wt% of the total weight of the pesticide composition, the compound of formula I and profenofos account for 0.5% to 80% of the total weight of the acaricidal composition;

[0013] Furthermore, the pesticide composition contains, in addition to the active ingredient, auxiliary ingredients permitted in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

[0014] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0015] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0016] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0017] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0018] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0019] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0020] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0021] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0022] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0023] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0024] Synergists are selected from synergist, piperonyl butoxide; and / or

[0025] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0026] Furthermore, the pesticide composition is prepared into a formulation acceptable for pesticides, and the formulation is a solid formulation and / or a liquid formulation;

[0027] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0028] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0029] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is an emulsifiable concentrate, a microemulsion or a suspoemulsion.

[0030] The present invention also discloses the use of the above pesticide composition and / or its preparation for preventing and controlling agricultural pests.

[0031] Furthermore, the harmful mites are from the family Tetranychus.

[0032] Furthermore, the pest mites of the family Tetranychidae include Tetranychus urticae, Tetranychus cinnabarinus, Panonychus citri, Tetranychus truncatus, Tetranychus citri, Tetranychus kanzawa, Panonychus apicale, Panonychus citri and / or Rock mite;

[0033] Preferably, the Tetranychus pest mite is Tetranychus urticae, Tetranychus cinnabarinus or Panonychus citri;

[0034] Furthermore, the pesticide composition and / or its preparation is applied to the living environment of agricultural pest mites or to crops where agricultural pest mites occur.

[0035] The beneficial effects of the present invention are as follows: the pesticide composition of the present invention is composed of two active ingredients with different action mechanisms, which overcomes the inherent defects of a single agent, has a significant synergistic effect on pest mites, is beneficial for delaying the resistance of pest mites to pesticides, and expands the control spectrum; improves the control effect, reduces the dosage of the active ingredient of the pesticide, improves the long-lasting effect, reduces the number of pesticide applications during the crop growing season, reduces agricultural costs, and reduces environmental pollution. DETAILED DESCRIPTION

[0036] In order to better understand the essence of the present invention, the present invention is further described below in conjunction with specific preparation examples and embodiments, but they should not be regarded as limiting the present invention.

[0037] Preparation Example 1: 24% Formula I compound·Profenofos emulsifiable concentrate (1:5)

[0038] Formula composition: 4% compound of formula I, 20% profenofos, 18% propylene glycol methyl ether, 12% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 10% cyclohexanone, 2% epoxidized soybean oil, and methyl oleate makes up the balance;

[0039] Preparation method: according to the formula ratio, the measured active ingredients, solvent and cosolvent are added into a mixing kettle and stirred to dissolve them, then the emulsifier is added and the balance is supplemented with the remaining solvent, and the mixture is stirred evenly in a stirring kettle. After filtering, the desired emulsifiable concentrate of the present invention is obtained.

[0040] Preparation Example 2: 20% Formula I compound·Profenofos microemulsion (1:3)

[0041] Formula composition: 5% compound of formula I, 15% profenofos, 10% cyclohexanone, 2% epoxy soybean oil, 10% styrylphenol polyoxyethylene ether, 2% arylphenol polyoxyethylene ether phosphate, 2% fatty alcohol polyoxyethylene ether sodium sulfate, 2% calcium dodecylbenzenesulfonate, 0.2% organosilicon defoamer, and deionized water to make up the balance;

[0042] Preparation method: According to the formula ratio, the active ingredient is completely dissolved in the solvent, and the emulsifier is added to form the oil phase. The dispersant, deionized water, etc. are stirred evenly to form the water phase; the oil phase is added to the water phase and stirred evenly, and high-speed shearing is performed until the particle size meets the requirements. After adding the defoaming agent, stir evenly to obtain the microemulsion product.

[0043] Preparation Example 3: 40% Formula I compound·Profenofos suspoemulsion (1:7)

[0044] Formula composition: 5% compound of formula I, 35% profenofos, 2% alkylphenol polyoxyethylene ether, 1% sodium salt of polycarboxylate, 4% styrenephenol polyoxyethylene ether sulfate, 2% Guerbet alcohol polyoxyethylene ether, 1% fumed silica, 2% styrenephenol polyoxyethylene ether methacrylate, 2% epoxidized soybean oil, 0.5% magnesium aluminum silicate, 0.2% xanthan gum, 4% propylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0045] Preparation method: According to the formula ratio, the compound of formula I is completely dissolved with solvent to prepare an oil phase, and chlorfluazuron is mixed with a wetting dispersant, an antifreeze agent, a defoaming agent, deionized water, etc. by wet sand grinding to D 90 The particles are smaller than 5 μm and a suspension mother liquor is prepared; the oil phase is added to the suspension mother liquor and stirred evenly, and then a thickener is added and stirred and sheared evenly to prepare a suspoemulsion product.

[0046] Preparation Example 4: 33% Formula I compound·Profenofos wettable powder (10:1)

[0047] Formula composition: 30% compound of formula I, 3% profenofos, 2% epoxidized soybean oil, 4% sodium lignin sulfonate, 5% formaldehyde condensate of sodium methylnaphthalene sulfonate, 2% pulverized powder BX, 5% white carbon black, and kaolin to make up the balance;

[0048] Preparation method: According to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0049] Preparation Example 5: 15% Formula I compound·Profenofos water dispersible granules (1:2)

[0050] Formula composition: 5% compound of formula I, 10% profenofos, 10% naphthalenesulfonate formaldehyde condensate, 6% sodium ligninsulfonate, 2% sodium lauryl sulfate, 2% epoxidized soybean oil, 10% ammonium sulfate, 10% starch, 10% white carbon black, and kaolin to make up the balance;

[0051] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then the mixture is kneaded, granulated, dried, and sieved to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0052] Example 1: Indoor biological activity assay

[0053] The indoor test determination refers to NY / T 1154.12-2008 "Test Guidelines for Indoor Biological Activity Determination of Pesticides - Insecticides Part 12: Spider Mite Slide Dipping Method".

[0054] Experimental equipment: stereo microscope, glass slide, white porcelain plate, double-sided tape, constant temperature incubator, etc.

[0055] Biological test materials: female adult mites of Tetranychus cinnabarinus Boisduval and Panonychus citri McGregor.

[0056] Test agent and preparation: The technical drug of the compound of formula I and the technical drug of profenofos were prepared into mother liquor with an organic solvent, and then prepared into 5 series of mass concentrations according to the equal ratio method with 0.1% Tween-80 aqueous solution.

[0057] Preparation of test materials: Cut double-sided tape into 2 cm lengths and stick them to one end of a glass slide. Then select healthy test mites and stick their backs to the double-sided tape, 30 mites per slide. Place them in a container lined with a wet sponge, cover with a lid, and place at (25±1)°C. After 2 hours, perform microscopic examination, remove dead and injured individuals, and replenish 30 mites per slide.

[0058] Chemical treatment: Immerse the slide in the chemical solution and gently shake for 5 seconds. Remove the slide and remove the excess solution with absorbent paper. Place the slide on a white porcelain plate lined with a damp sponge and cover with light-transmitting plastic film. Repeat each treatment four times, and include a treatment without chemical (including all organic solvents and emulsifiers) as a blank control.

[0059] Rearing and observation: The container containing the test mites was placed at (25±1)℃ and the photoperiod was L:D=(16:8)h for rearing and observation.

[0060] Inspection: 48 hours after treatment, check the mortality of test mites and record the total number of mites and the number of dead mites respectively.

[0061] Data statistics and analysis:

[0062] Based on the survey data, the adjusted mortality rate of each treatment was calculated according to the following formula, and the calculation results were rounded to two decimal places.

[0063]

[0064] Where:

[0065] P——mortality rate, in percentage (%);

[0066] K——indicates the number of dead insects, the unit is head;

[0067] N——represents the total number of insects processed, in heads.

[0068]

[0069] Where:

[0070] P1——adjusted mortality rate, in percentage (%);

[0071] P t ——Treatment mortality rate, expressed in percentage (%);

[0072] P0 - blank control mortality rate, in percentage (%).

[0073] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0074] Analyze using the DPS statistical analysis system to determine the LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0075] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0076]

[0077] Where:

[0078] ATI - measured toxicity index of mixture;

[0079] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0080] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0081] TTI=TI A ×P A +TI B ×P B

[0082] Where:

[0083] TTI – Theoretical Toxicity Index of Mixtures;

[0084] TI A ——Agent toxicity index;

[0085] P A ——The percentage of agent A in the mixture, in percentage (%);

[0086] TI B ——Toxicity index of agent B;

[0087] P B——The percentage of agent B in the mixture, in percentage (%).

[0088]

[0089] Where:

[0090] CTC – Co-toxicity coefficient;

[0091] ATI - measured toxicity index of mixture;

[0092] TTI - Theoretical Toxicity Index of Mixture.

[0093] A co-toxicity coefficient (CTC) of the combination of CTC≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0094] Indoor activity test is shown in the table below:

[0095] Table 1 Results of indoor biological activity determination test of Tetranychus cinnabarinus

[0096]

[0097]

[0098] Table 2 Results of indoor bioactivity determination test of Panonychus citri

[0099]

[0100] The results of the indoor tests show (Tables 1 and 2) that the compound of formula I and profenofos, when combined in an appropriate mass ratio, have a significant synergistic effect against Tetranychus cinnabarinus or Panonychus citri.

[0101] The mass ratio of the compound of formula I to profenofos is 1:40 to 10:1, and the co-toxicity coefficient to Tetranychus cinnabarinus is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to profenofos is 1:30 to 2:1, and the co-toxicity coefficient is greater than 140, showing an obvious synergistic effect.

[0102] The mass ratio of the compound of formula I to profenofos is 1:25-45:1, and the co-toxicity coefficient to citrus mites is greater than 120, showing a synergistic effect; the mass ratio of the two is 1:15-25:1, and the co-toxicity coefficient to citrus mites is greater than 130, showing a significant synergistic effect.

[0103] Example 2: Field efficacy test for controlling citrus red spider mites

[0104] The test was conducted with reference to GB / T 17980.11-2000 "Guidelines for field efficacy tests of pesticides (I) Acaricides for the control of Panonychus citri"

[0105] Test subject: Panonychus citri.

[0106] Experimental crops: Citrus (Spring See).

[0107] Experimental environmental conditions: The experiment was conducted in a citrus plantation in Shizishu Village, Heshan Street, Pujiang County, Chengdu City, Sichuan Province. The cultivation conditions in the experimental garden were uniform and consistent, in line with local scientific agricultural practices (GAP).

[0108] Experimental Design: Four treatments were set up, each replicated four times, for a total of 16 plots, with two citrus trees in each plot. On November 17, 2023, a backpack electric sprayer was used to evenly spray the entire tree, ensuring that the leaves, leaf backs, branches, and fruit surfaces were sprayed thoroughly, without overspraying, and that the entire tree was moist without dripping.

[0109] Experimental investigation: Investigate the base number of mites before the experiment, and investigate once 3 days and 15 days after the drug application, and record the number of active mites.

[0110] Calculation method of drug efficacy:

[0111]

[0112]

[0113] The test results are shown in the table below:

[0114] Table 3 Results of field trials on the efficacy of pesticides against citrus red spider mites

[0115]

[0116] The results of the field efficacy test showed that the control effect of 24% Formula I compound·Profenofos emulsifiable concentrate (1:5) was significantly higher than that of the single-dose control 3 days and 10 days after application, showing good rapid and long-lasting effects.

[0117] Example 3: Field efficacy test for controlling spider mites in loofah

[0118] The test was carried out in accordance with GB / T 17980.17-2000 “Guidelines for field efficacy tests of pesticides (I) Acaricides for the control of spider mites on beans and vegetables”.

[0119] Experimental area: loofah fields in Houmoucheng Village, Shouguang City, Shandong Province. The soil fertility of the experimental site was above average, and the cultivation conditions were the same in all experimental plots.

[0120] Test target: Tetranychus cinnabarinus.

[0121] Experimental crop: loofah.

[0122] Test method: Each test plot has an area of ​​50m 24 replicates, totaling 16 plots. The experiment involved a single application at the early stage of spider mite infestation. A baseline mite population was surveyed before the experiment and again 3, 7, and 14 days after application. Twenty leaves were collected from each plot to count the number of live and nymphal mites.

[0123] Calculation method of drug efficacy:

[0124]

[0125]

[0126] The results of the field efficacy test are shown in the table below:

[0127] Table 4 Field efficacy test results for controlling spider mites in loofah

[0128]

[0129] As shown in the test results in Table 4, the rational combination of the compound of Formula I and profenofos demonstrated excellent control of Tetranychus cinnabarinus, effectively controlling the mite's infestation. Three days after application, the control efficacy was significantly higher than that of the single-dose control, demonstrating rapid efficacy. Fourteen days after application, the combined formulation achieved a control efficacy of 91.61%, demonstrating a long-lasting effect.

[0130] During the above field efficacy tests, irregular observations were conducted, and no visible phytotoxicity symptoms were found in the test crops caused by the formulations in the examples, and the crops grew well after application.

[0131] It should be understood that the above embodiments are only some of the embodiments of the present invention and are provided only for a better understanding of the embodiments of the present invention. They do not constitute all embodiments of the present invention. In actual applications, by adjusting the content of each component of the present invention and the composition of the components, various and countless embodiments can be obtained, all of which are within the scope of the present invention.

Claims

1. A pesticide composition containing profenofos, characterized in that: The active ingredient of the pesticide composition comprises a compound of formula I: (Formula I) and profenofos, wherein the mass ratio of the compound of Formula I to profenofos is 1:25 to 45:

1.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of the compound of formula I to profenofos is 1:20 to 10:

1.

3. The pesticide composition according to claim 1, characterized in that The total weight of the pesticide composition is 100 wt%, and the compound of formula I and profenofos account for 0.5% to 80% of the total weight of the acaricidal composition.

4. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also contains auxiliary ingredients allowed in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The pesticide composition according to claim 1, characterized in that The pesticide composition is prepared into a formulation acceptable for pesticides, and the formulation is a solid formulation and / or a liquid formulation.

6. The pesticide composition according to claim 5, characterized in that The solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is an emulsifiable concentrate, a microemulsion or a suspoemulsion.

7. Use of the pesticide composition according to any one of claims 1 to 6 for controlling agricultural pests, characterized in that: The harmful mites are Tetranychus cinnabarinus and Panonychus citri.

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