Traditional Chinese medicine composition for preventing and / or treating chicken adenomyotic gastritis, and preparation method and application thereof

Abstract

The present invention relates to the field of veterinary medicine technology, specifically a Chinese medicine composition for preventing and / or treating chicken adenomyosis and its preparation method and application. The Chinese medicine composition is composed of Sophora flavescens, Patchouli, Radix Et Rhizoma Rhei, Radix Astragali, Scorched Crataegus and Areca nut. It has the effects of clearing away heat and dampness, invigorating the spleen and appetizing, widening the intestine and removing stagnation, and can effectively treat poultry anorexia, indigestion, abdominal distension and other diseases. For the frequent chickens of adenomyosis, it is used before the onset of age, and can effectively prevent the occurrence of adenomyosis, ensure that the whole feeding cycle of chicken does not drop material, reduce the overall feed-to-meat ratio, and increase breeding economic benefits. In addition, the medicinal material cost used for 1 treatment cycle of 10,000 broilers (20 days old) is 80-120 yuan, and cost is low and pure Chinese medicine preparation is safe.

Description

Technical Field

[0001] The present application relates to the field of veterinary medicine technology, and specifically to a traditional Chinese medicine composition for preventing and / or treating chicken glandular gastritis, and a preparation method and application thereof. Background Art

[0002] Glandular gastritis in chickens is a contagious disease characterized by enlarged glandular stomachs and erosive and ulcerated gizzards. Clinical features include decreased appetite, slow growth, and rough, dull, and unkempt feathers. Typical wing feathers are ruffled, partially broken, and propeller-shaped. Other symptoms include poor growth, emaciation, and poor stamina. Severely affected birds weigh only 1 / 5 to 1 / 10 of healthy birds. Diarrhea and undigested feed can also occur in the feces. Autopsy reveals enlarged glandular stomachs, such as milky white globules, or flattened or even absent papillae. The glandular stomach mucosa is bleeding, ulcerated, and sloughed. The gizzard mucosa also presents with crater-like ulcers and erosions. The disease is not breed or age-specific, affecting all breeds and ages. It can occur as early as one-day-old chicks and as early as 300-day-old laying hens. It is most common in commercial broilers between 7 and 30 days old. The younger the age of onset, the more severe the disease. In areas where this disease is common, once a chicken farm is infected, every batch will be affected, with an incidence rate of up to 100%. The disease is non-seasonal and can occur year-round, with peak severity from June to September. It is often sporadic and can easily be mixed with or secondary to other diseases. In recent years, the disease has become widespread in my country, with an increasing incidence rate. It is particularly severe in white-feathered broilers and the 817 breed, making its control crucial for the economic success of chicken farming.

[0003] There are many different opinions on the cause of this disease. It is generally believed that this disease is a syndrome caused by one or more infectious pathogens and non-infectious factors, with the digestive tract and endocrine organs as the target organs of the pathogenic factors. Due to the complex etiology, multiple factors such as pathogenic microorganisms, breeding environment, feed, and feeding management all affect the occurrence of the disease. There is no specific treatment drug, so once a chicken farm is infected with this disease, the losses are huge. Currently, the treatment options for this disease mainly rely on chemical drugs, traditional Chinese medicine preparations, and even compound preparations of the two. The problems are as follows:

[0004] (1) Chemical drugs: antibiotics such as amoxicillin and neomycin are commonly used to improve symptoms and alleviate the condition. However, this solution does not eliminate all pathogenic factors, but only alleviates symptoms. The treatment is not thorough and is prone to relapse. In addition, improper use of antimicrobial drugs will promote drug resistance and affect the treatment of other diseases. Moreover, the use of antimicrobial drugs before broiler chickens are marketed and during the laying period of laying hens will produce drug residues, affecting food safety.

[0005] (2) Traditional Chinese medicine preparations have no toxic side effects or residual effects, so many materials report the use of traditional Chinese medicine to prevent and treat chicken glandular gastritis. However, in the existing technology, there are still a series of problems in using traditional Chinese medicine to treat chicken infectious glandular gastritis. First, these prescriptions are usually large, mostly composed of more than 8 traditional Chinese medicines, and the quality is difficult to control, which brings inconvenience to production and use; secondly, the effects of these prescriptions are uncertain and need to be verified; thirdly, the prices of some of these medicines are relatively expensive, such as honeysuckle, atractylodes, forsythia, and whitehead, with a unit price of 100-200 yuan / kg, which is clinically unacceptable.

[0006] Therefore, it is urgent to develop a Chinese medicine preparation with definite efficacy, few medicinal ingredients, easy production and use, and low cost for preventing and treating chicken glandular gastritis.

[0007] In view of this, the present invention is proposed. Summary of the Invention

[0008] The first object of the present invention is to provide a traditional Chinese medicine composition for preventing and / or treating chicken adenomyotic gastritis.

[0009] The second object of the present invention is to provide a method for preparing the above-mentioned Chinese medicine composition.

[0010] The third object of the present invention is to provide a Chinese medicine preparation.

[0011] The fourth object of the present invention is to provide the application of the above-mentioned Chinese medicine composition or Chinese medicine preparation.

[0012] In order to achieve the above object, the present invention adopts the following technical solutions:

[0013] A traditional Chinese medicine composition for preventing and / or treating chicken glandular gastritis consists of the following components: sophora flavescens, patchouli, rhubarb, astragalus, charred hawthorn and betel nut.

[0014] Furthermore, the Chinese medicine composition is composed of the following components by weight: 3-8 parts of Sophora flavescens, 1-5 parts of Patchouli, 2-6 parts of Rhubarb, 1-5 parts of Astragalus, 1-5 parts of Charred Crataegus, and 1-3 parts of Areca catechu.

[0015] Furthermore, the Chinese medicine composition is composed of the following components by weight: 4 to 6 parts of Sophora flavescens, 2 to 4 parts of Patchouli, 3 to 5 parts of Rhubarb, 2 to 4 parts of Astragalus, 2 to 4 parts of charred Crataegus, and 1 to 3 parts of Areca catechu.

[0016] Furthermore, the Chinese medicine composition is prepared into a preparation suitable for oral administration;

[0017] Preferably, the preparation suitable for oral administration is a liquid preparation suitable for oral administration.

[0018] A method for preparing the above-mentioned Chinese medicine composition, the preparation method comprising the following steps:

[0019] (a) soaking Patchouli in water and then steam distilling to extract volatile oil;

[0020] (b) boiling Sophora flavescens, Rhubarb, Astragalus, Crataegus pinnatifida, and Areca catechu with water and collecting the filtrate;

[0021] (c) mixing the volatile oil in (a) and the filtrate in (b) to obtain the traditional Chinese medicine composition.

[0022] Furthermore, the amount of water used to soak Patchouli in (a) is 8 to 12 times the weight of Patchouli, the soaking time is 0.5 to 1.5 hours, and the extraction time is 7 to 10 hours.

[0023] Furthermore, the amount of water added in (b) is 8 to 12 times the total weight of Sophora flavescens, Rhubarb, Astragalus, Charred Hawthorn and Areca catechu, the number of decoctions is 2 to 4 times, and the decoction time is 1.5 to 2.5 hours.

[0024] Furthermore, in (c), the filtrate is first concentrated to a relative density of 1.05 to 1.1 at 50 to 60° C., and then the volatile oil in (a) is added and mixed to obtain the traditional Chinese medicine composition.

[0025] A traditional Chinese medicine preparation is prepared from the above-mentioned traditional Chinese medicine composition, and the dosage form of the traditional Chinese medicine preparation includes solid, semi-solid or liquid dosage form.

[0026] Application of the above-mentioned Chinese medicine composition or Chinese medicine preparation in the prevention and / or treatment of chicken adenomyotic gastritis.

[0027] Preferably, the drug is used as a mixed drink.

[0028] Preferably, the therapeutic dosage of the drug is 1.0 to 1.25 ml per liter of water, for 5 to 7 consecutive days.

[0029] Preferably, the preventive dosage of the drug is 0.8 to 1.2 ml per liter of water, for 7 to 10 consecutive days.

[0030] Compared with the prior art, the technical effects of the present invention are:

[0031] The Chinese medicine composition of the present invention has the effects of clearing away heat and dampness, strengthening the spleen and appetizing, widening the intestine and resolving stagnation, and can effectively treat symptoms such as loss of appetite, indigestion, and abdominal distension in poultry. In addition, it can also balance the immune system, promote the growth of livestock, and has a certain preventive and therapeutic effect on common adenomyosis and enteritis in livestock. For chickens infected with adenomyosis, the feed intake can be significantly increased, the mortality rate can be reduced, and the animal body can be quickly recovered. For chickens that often suffer from adenomyosis, using it before the age of onset can effectively prevent the occurrence of adenomyosis, ensure that the chickens do not drop feed during the entire breeding cycle, reduce the overall feed-to-meat ratio, and increase the economic benefits of breeding.

[0032] The Chinese medicine composition of the present invention is a pure Chinese medicine preparation with no drug residue and no toxic side effects; the whole formula consists of only 6 medicinal ingredients, and the quality is easy to control; compared with other solutions, it has a high cost-effectiveness ratio, the price of medicinal materials is 8-12 yuan / kg, and the cost of medicinal materials used for one treatment cycle of 10,000 broilers (20 days old) is 80-120 yuan, which is easily acceptable to farms. DETAILED DESCRIPTION

[0033] Unless otherwise defined, all technical and scientific terms used in this application are the same as those generally understood by those skilled in the art to which this application belongs. In the event of any inconsistency, the meaning described in the full text of this application or the meaning derived from the content recorded in the full text of this application shall prevail. In addition, the terms used in this description are only for the purpose of describing the embodiments of the present application and are not intended to limit this application. Below, the specific embodiments of the present invention are described in detail.

[0034] Although the numerical ranges and parameters used to define the broader scope of the present invention are approximate, the numerical values ​​of the specific examples are presented herein as precisely as possible. However, any numerical value inherently and inevitably contains standard deviations due to individual testing methods. As used herein, "about" generally means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a particular value or range. Alternatively, the term "about" means that the actual value falls within an acceptable standard error of the mean, as determined by one skilled in the art. Except in the experimental examples, or unless otherwise expressly indicated, all ranges, amounts, values, and percentages used herein (e.g., to describe material amounts, time periods, temperatures, operating conditions, quantitative ratios, and the like) are to be understood as modified by the word "about." Therefore, unless otherwise indicated, the numerical parameters disclosed in this specification and the accompanying claims are approximate and are subject to change as needed. At a minimum, these numerical parameters should be understood to include the number of significant digits indicated and to apply normal rounding. Herein, numerical ranges are expressed from one end point to another or between two end points; unless otherwise stated, the numerical ranges described herein include the end points.

[0035] "Treatment" in the present invention refers to applying or administering the pharmaceutical composition of the present invention to chickens suffering from symptoms, secondary diseases or signs associated with chicken adenomyosis, so as to partially or completely alleviate, slow down, cure the disease, delay the onset of the disease, inhibit the progression of the disease, reduce the severity of the disease, and / or reduce the occurrence of one or more symptoms, secondary diseases or signs associated with chicken adenomyosis.

[0036] In the present invention, "prevention" refers to delaying the onset of symptoms of chickens suffering from adenomyotic gastritis or reducing the occurrence of chicken adenomyotic gastritis.

[0037] The traditional Chinese medicine composition provided by the present invention can be used for preventing and / or treating chicken glandular gastritis, and contains only six traditional Chinese medicines, namely, sophora flavescens, patchouli, rhubarb, astragalus, charred hawthorn and betel nut, as effective ingredients.

[0038] Sophora flavescens, also known as bitter bone, ground bone, or cow ginseng, is the root of the perennial deciduous subshrub Sophora flavescens, a Leguminosae plant. It is bitter and cold in nature. It enters the heart, liver, stomach, large intestine, and bladder meridians. It clears heat and dampness, kills insects, and acts as a diuretic. It contains numerous alkaloids and flavonoids. The main alkaloids are matrine and oxymatrine, with other compounds including isomaltine, sophorocarpine, isosophorocarpine, sophoramine, and oxysophorocarpine, as well as trace amounts of d-sophorolamine, l-soybean, l-methylcytisine, pseudoindigotine, and l-sophorrheic acid. Among the flavonoids are pyrethrol C, pyrethrol G, isomatrine, matrine, neomatrine, normatrine, formononetin, matrine, matrine, and hypomatrine.

[0039] Patchouli is the whole herb of Patchouli or Patchouli, a plant of the Lamiaceae family. It is pungent and warming. It enters the spleen, stomach, and lung meridians. It has the effects of eliminating dampness and invigorating the spleen, dispelling filth and harmonizing the stomach, and relieving summer heat. Patchouli contains volatile oils, the main components of which are patchouli ketone and patchouli alcohol, with other components including benzaldehyde, eugenol, and cinnamaldehyde. Patchouli also contains volatile oils, the main component of which is methylchavicol, as well as anisole, anisaldehyde, d-limonene, p-methoxycinnamaldehyde, α-pinene, β-pinene, 3-octanone, 3-octanol, and p-cymene.

[0040] Rhubarb, also known as General, Sichuan Army, or Brocade-patterned Rhubarb, is the dried root and rhizome of Rheum palmatum, Rheum tanguticum, or Rheum officinale, all from the Polygonaceae family. It has a bitter and cold nature and enters the spleen, stomach, large intestine, pericardium, and liver meridians. It has the effects of purging, clearing away heat and purging fire, cooling blood and detoxifying, removing blood stasis and promoting menstruation, and promoting the removal of dampness and jaundice.

[0041] Astragalus, also known as Beiqi (Northern Astragalus), Mianqi (Mianqi), or Huangqi (Huangqi), is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. It has a sweet and slightly warm nature and enters the spleen and lung meridians. It has the effects of tonifying qi and raising yang, strengthening the body's defense system, promoting diuresis and reducing swelling, promoting fluid production and nourishing blood, relieving stagnation and numbness, expelling toxins and pus, and healing sores and promoting tissue regeneration.

[0042] Hawthorn is the dried, mature fruit of the plant Crataegus pinnatifida (Hawthorn) in the Rosaceae family. Clean hawthorns are stir-fried according to the stir-frying method (Chinese Pharmacopoeia 2020 Edition, Part IV, General Chapter 0213) until the surface is burnt brown and the interior is yellowish brown. This is charred hawthorn. Charred hawthorn shares similar properties and flavors with hawthorn: sour, sweet, and slightly warm, it enters the spleen, stomach, and liver meridians. It has benefits such as digestion, stomach strengthening, and promoting qi and dispersing blood stasis. Charred hawthorn is more effective in digestion and relieving stagnation than unburned hawthorn.

[0043] Betel nut, also known as betel nut, betel nut, betel nut, betel nut, or betel nut, is the dried mature seed of the palm plant Areca catechu. It has a bitter, pungent, and warming taste. It enters the stomach and large intestine meridians. It has insecticidal, digestive, qi-promoting, diuretic, and antimalarial properties.

[0044] In a preferred embodiment, the Chinese medicine composition is composed of the following components by weight: 3-8 parts of Sophora flavescens, 1-5 parts of Patchouli, 2-6 parts of Rhubarb, 1-5 parts of Astragalus, 1-5 parts of Charred Crataegus, and 1-3 parts of Areca. The content of Sophora flavescens can be, but is not limited to, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, or 8 parts; the content of Patchouli can be, but is not limited to, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts; the content of Rhubarb can be, but is not limited to, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts , 5, 5.5 or 6 parts; the content of Astragalus can be but not limited to 1 part, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5 parts; the content of charred hawthorn can be but not limited to 1 part, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5 parts; the content of Betel nut can be but not limited to 1 part, 1.5, 2, 2.5 or 3 parts.

[0045] In a more preferred embodiment, the Chinese medicine composition consists of the following components by weight: 4-6 parts of Sophora flavescens, 2-4 parts of Patchouli, 3-5 parts of Rhubarb, 2-4 parts of Astragalus, 2-4 parts of charred Crataegus, and 1-3 parts of Areca catechu.

[0046] The present invention provides a traditional Chinese medicine preparation, which is prepared from the above-mentioned traditional Chinese medicine composition. Depending on the purpose of implementation, the traditional Chinese medicine preparation can be formulated with a pharmaceutically acceptable carrier or excipient, such as a solid, semi-solid or liquid dosage form, such as pills, tablets, powders, pastes, granules or ointments.

[0047] In a preferred embodiment, the Chinese medicine preparation of the present invention is a preparation suitable for oral administration, more preferably a liquid preparation.

[0048] The present invention provides a method for preparing a traditional Chinese medicine composition, comprising the following steps:

[0049] (a) soaking Patchouli in water and then steam distilling to extract volatile oil; preferably, the amount of water used to soak Patchouli in (a) is 8 to 12 times the weight of Patchouli, the soaking time is 0.5 to 1.5 hours, and the extraction time is 7 to 10 hours. For example, taking Patchouli, soaking it in 10 times the amount of water for 1 hour, and then steam distilling and extracting for 8 hours to collect the volatile oil.

[0050] (b) Sophora flavescens, rhubarb, astragalus, charred hawthorn, and areca catechu are decocted with water and the filtrate is collected; preferably, the amount of water added in (b) is 8 to 12 times the total weight of Sophora flavescens, rhubarb, astragalus, charred hawthorn, and areca catechu, the decoction is repeated 2 to 4 times, and the decoction time is 1.5 to 2.5 hours. For example, the other five herbs, excluding Patchouli, are decocted with water twice, each time adding 10 times the amount of water, and the decoction is continued for 2 hours. After each extraction, the filtrate is filtered and collected.

[0051] (c) Mixing the volatile oil in (a) and the filtrate in (b) to obtain a Chinese medicine composition. Preferably, the filtrate is first concentrated to a relative density of 1.05 to 1.1 at 50 to 60° C., then the volatile oil in (a) is added, mixed, fixed to volume, and bottled to obtain the Chinese medicine composition.

[0052] The present invention provides the use of the aforementioned traditional Chinese medicine composition or preparation in the prevention and / or treatment of adenomyotic gastritis in chickens. The use comprises administering an effective amount of the drug to chickens. Administration is preferably by oral administration, and more preferably by mixing with drinking water.

[0053] In some embodiments, the therapeutic dosage of the drug is 1.0 to 1.25 ml per liter of water, for continuous use for 5 to 7 days.

[0054] In some embodiments, the preventive dosage of the drug is 0.8 to 1.2 ml per liter of water, for 7 to 10 consecutive days.

[0055] Example 1 Chinese medicine composition and its preparation

[0056] 1.1 Compositions and Preparation Methods of the Present Invention

[0057] Composition 1: 4 parts of Sophora flavescens, 2 parts of Patchouli, 3 parts of Rhubarb, 4 parts of Astragalus, 4 parts of Charred Crataegus, and 3 parts of Areca.

[0058] Composition 2: 5 parts of Sophora flavescens, 3 parts of Patchouli, 4 parts of Rhubarb, 3 parts of Astragalus, 3 parts of Charred Crataegus, and 2 parts of Areca catechu.

[0059] Composition 3: 6 parts of Sophora flavescens, 4 parts of Patchouli, 3 parts of Rhubarb, 2 parts of Astragalus, 2 parts of Charred Crataegus, and 1 part of Areca catechu.

[0060] Preparation method:

[0061] For composition 1, 2, or 3, weigh the herbs in proportion. Take Patchouli Radix, soak it in 10-fold the amount of water for 1 hour, then steam distill it for 8 hours and collect the volatile oil. Decoction the other five herbs twice, adding 10-fold the amount of water each time, and boil for 2 hours. After each extraction, filter and collect the filtrate. Concentrate the filtrate to a relative density of 1.05-1.10 (at 50-60°C), add the volatile oil, and adjust the volume to a solution containing 1g of the original herb per 1ml. Fill into 250ml bottles and set aside.

[0062] 1.2 Comparative Example Formula and Preparation Method

[0063] Comparative Example 1: From Chinese Patent CN107184702A

[0064] Take 15 parts of honeysuckle, 15 parts of astragalus, 30 parts of kudzu root, 25 parts of liquorice, 10 parts of dried tangerine peel, 15 parts of gardenia, and 15 parts of corydalis, grind them into coarse powder with a particle size of 180 μm to 600 μm, add 4 times the weight of water after combining, and soak for 2 hours; add purified water to 12 times the weight of the medicinal materials after soaking and perform ultrasonic extraction; combine the medicinal material extracts, use vacuum negative pressure concentration method to concentrate the medicinal liquid to the drug After the volume of the extract is reduced to twice the volume of the raw material, the extract is poured out and the extract is placed in a 4°C cold storage for 24 hours; the extract of the raw material that has been placed at 4°C for 24 hours is taken out and subjected to online high-speed centrifugation at 12,000 rpm; (5) the pH value of the centrifuge is adjusted to 5, and filtered using a 0.22 μm to 0.45 μm microporous filter membrane, the filtrate is collected, and the filtrate is prepared and bottled to prepare a 1 ml solution containing 1 g of the original raw material, and the solution is filled into 250 ml / bottle for later use.

[0065] Comparative Example 2: From Chinese Patent CN105663546A

[0066] Weigh 120 parts of Codonopsis pilosula, 60 parts of cinnamon bark, 50 parts of Astragalus membranaceus, 40 parts of Atractylodes macrocephala, 50 parts of chicken's gizzard lining, 40 parts of Areca catechu, 40 parts of montmorillonite, 40 parts of cuttlefish shell, 50 parts of moutan bark, and 30 parts of liquorice by weight, grind them, and put them into bags for later use.

[0067] Comparative Example 3: 10% amoxicillin soluble powder and cimetidine tablets were fed for treatment for 5 consecutive days.

[0068] Example 2 Evaluation of the effects of different compositions in treating adenomyotic gastritis in broiler chickens

[0069] 2.1 Case diagnosis

[0070] A farm in Yanshi District, Luoyang City, Henan Province, raised approximately 26,000 AA broiler chickens in cages, using commercially available complete feed. At 13 days of age, the chickens experienced decreased feed intake, diarrhea, undigested feed in their feces, and mortality, with 12 birds dying that day. The dead birds were emaciated, with dirty feathers. Autopsies of the dead birds revealed severe swelling of the glandular stomach, thickening of the stomach wall, and a lattice-like texture upon dissection. The glandular stomach mucosa was sloughed and ulcerated, and the stratum corneum of the gizzard was ulcerated. Based on the onset and autopsy findings, the disease was diagnosed as glandular myogastritis.

[0071] 2.2 Experimental methods and efficacy evaluation indicators

[0072] 2.2.1 Method for selecting sick chickens

[0073] Thirty minutes after the first morning feeding, 216 chickens with flat crops, low body weight, and poor spirits were selected from the flocks diagnosed with glandular gastritis and placed individually. The birds were numbered with footmarks. For five consecutive times, 30 minutes after each feeding, the crops of the birds were touched to inspect the food content and to assess their feeding status (a score of 2 for full crops, 1 for food but not full, and 0 for no food). Birds with a total score of 3 or less for the five times were entered into the experiment.

[0074] 2.2.2 Trial Grouping and Dosing

[0075] 175 eligible sick chickens were selected and randomly divided into seven groups of 25 chickens each using a random number table. The seven groups were Group A, Group B, Group C, Group D, Group E, Group F, and Group G. Twenty-five chickens with full crops 30 minutes after feeding were selected from the flock as a healthy control group, designated Group H. Each group was administered medication according to the schedule in Table 1, and indicators were evaluated.

[0076] Table 1 Experimental treatments

[0077]

[0078] 2.2.3 Evaluation indicators

[0079] 2.2.3.1 Serum inflammatory factors

[0080] After the administration, 20 chickens were randomly selected from each group, and blood was collected on an empty stomach. The serum was separated and the levels of serum gastrin (GAS), pepsinogen 1 (PG1), pepsinogen 2 (PG2) and C-reactive protein (CRP) were determined.

[0081] 2.2.3.2 Cure rate, efficacy and feeding score

[0082] On the second day after dosing, 30 minutes after the first feeding in the morning, observe and record the food content in the crop according to the method in 2.2.1 and score it. A score of 2 is considered cured, a score of 1 is considered effective, and a score of 0 or animal death is considered ineffective. The cure rate and effectiveness rate are calculated based on the scores.

[0083] Cure rate = number of animals cured / number of animals enrolled

[0084] Effectiveness = (number of effective animals + number of cured animals) / number of selected animals

[0085] 2.2.4 Result analysis method

[0086] The data were analyzed and processed using statistical software SPSS 19.0, where the average values ​​of feeding score, GAS, PGR (PG1 / PG2) and CRP content were used. The standard deviation (SD) was expressed and the differences between the groups were analyzed by one-way analysis of variance and Duncan comparison; the cure rate and effective rate were expressed as percentage (%) and the differences between the groups were analyzed by chi-square test.

[0087] 2.3 Test results

[0088] Table 2 Analysis results of each test evaluation index

[0089]

[0090] Note: In the same column, the numbers without the same lowercase letters in the shoulder indicate significant differences (P≤0.05), and the numbers without the same uppercase letters in the shoulder indicate extremely significant differences (P≤0.01).

[0091] As can be seen from Table 2, after treatment, the levels of inflammatory factors PGR, GAS, and CRP in the serum of the chickens in the drug-treated group were comparable to those in the healthy control group (P>0.05), among which PGR and GAS were significantly higher than those in the diseased non-drug treatment group (P≤0.05), and CRP was significantly lower than that in the diseased non-drug treatment group (P≤0.05), which were comparable to those in the healthy control group.

[0092] The cure rates of Composition 1, Composition 2, and Composition 3 for chicken glandular gastritis were 76%, 84%, and 72%, respectively, and the effective rates were 100%, respectively. The cure rates were significantly better than those of Comparative Examples 1, 2, and 3 (P≤0.05), and the effective rates were significantly better than those of Comparative Examples 1, 2, and 3 (P≤0.05). After using the composition of the present invention, the feed intake was significantly increased, and the feeding symptom score was significantly higher than that of Comparative Examples 1, 2, and 3.

[0093] The test results show that compositions 1 to 3 prepared using Example 1 of the present invention can treat reduced feed intake caused by adenomyotic gastritis in chickens and improve the content of inflammatory factors in serum, indicating that the traditional Chinese medicine composition of the present invention has a significant therapeutic effect on adenomyotic gastritis in chickens.

[0094] Example 3 Application of different compositions in treating adenomyotic gastritis in broiler chickens

[0095] 3.1 Incidence

[0096] A farm in Qi County, Kaifeng City, Henan Province, raises approximately 13,000 16-day-old 817 broiler chickens in cages. The coop has three rows of walkways and eight rows of cages, with two rows placed back-to-back, each row consisting of upper, middle, and lower layers. The chickens began to experience a decrease in feed intake at 11 days of age. By 16 days of age, they were only consuming 8 bags (320 kg) of feed, compared to a normal intake of 12 bags (480 kg), a 33.3% decrease in feed intake. Sporadic deaths occurred, with 15-20 chickens dying daily. Upon inspection at 16 days of age, the chickens appeared depressed, with ruffled feathers and some broken feathers. The feces were watery and contained undigested feed, with some cages containing long, thin feces. Autopsies of five dead chickens and two emaciated chickens revealed enlarged glandular stomachs. Opening revealed thickened and edematous glandular stomach walls, with fluid oozing out upon pressure. Some papillae were fused, with unclear boundaries. Most exhibited symptoms of enteritis. Two of the dead chickens had blackened stratum corneum and ulcers in the gizzards. Based on the onset and autopsy findings, the condition was diagnosed as glandular myogastritis.

[0097] 3.2 Experimental methods and efficacy evaluation indicators

[0098] The chickens were divided into 8 groups according to the position of the chicken cages in the chicken house, with each column as one group. From right to left, they were Group A (1587), Group B (1583), Group C (1585), Group D (1581), Group E (1596), Group F (1592), Group G (1594), and Group H (1593). Each group was treated with the drug according to the experimental treatment method in Table 1. During the treatment period, the status of the chickens in each group was observed daily, the number of deaths was recorded, and the feed intake was counted. The observation was continued until 2 days after the end of the drug treatment.

[0099] Table 3 Experimental treatments

[0100]

[0101] 3.3 Test results

[0102] The mortality and feed intake statistics of each group of chickens are shown in Table 4.

[0103] Table 4 Statistics of mortality and feed intake of chickens in each group during treatment

[0104]

[0105]

[0106] Note: In the mortality column, those with different lowercase letters on the shoulders indicate significant differences (P≤0.05), and those with different uppercase letters on the shoulders indicate extremely significant differences (P≤0.01).

[0107] As shown in Table 4, Group H, which did not receive any medication, experienced sporadic deaths daily throughout the experiment, resulting in a total of 14 deaths, for a mortality rate of 0.88%. Feed intake increased slightly with age, but remained low compared to the standard feed intake. By the end of the experiment, feed intake was only 66.9% of the standard feed intake, essentially unchanged from pre-experimental levels. Group G, after receiving amoxicillin, showed gradual improvement in mental status on day 4, and increased feed intake, but it did not return to normal levels. Sporadic deaths continued. Mortality in Group G decreased after medication, reaching a lower level than in Group H, which did not receive any medication. However, there was no significant difference between the two groups (P>0.05). Feed intake increased by the end of the experiment, but it still did not return to normal, reaching 78.5% of the standard feed intake.

[0108] After drug treatment, the mental state and feeding status of groups A to D showed a certain degree of improvement, with a significant improvement in mental state and increased feed intake. At the end of the medication, the feed intake basically reached the standard feed intake (96.0-100.7% of the standard feed intake), the number of dead chickens decreased, and the mortality rate was only 0.13%-0.25%. The mortality rates of groups A and D during the entire experimental period were significantly lower than those of group H without medication (P≤0.05), and those of groups B and C were significantly lower than those of group H without medication (P≤0.01). In groups E and F, the feed intake increased significantly after medication using the Chinese medicine composition prepared in the control example, and basically returned to normal at the end of the experiment, reaching 94.2% and 90.4% of the standard feed intake. The mortality rate during the experiment was significantly reduced, significantly lower than that of group H (P≤0.05), and there was no significant difference compared with groups A to D.

[0109] The test results show that compositions 1 to 3 prepared using Example 1 of the present invention can quickly restore the decreased feed intake caused by adenomyotic gastritis in chickens and significantly reduce their mortality rate, indicating that the traditional Chinese medicine composition of the present invention has a significant therapeutic effect on adenomyotic gastritis in chickens.

[0110] Example 4 Application of different compositions in broiler chickens with adenomyotic gastritis

[0111] 4.1 Incidence

[0112] A farmer in Weishi, Kaifeng, Henan Province, raised 8,500 AA broiler chickens, housed on a flat surface. Starting at 9 days of age, their daily feed intake remained essentially unchanged. On-site observations at 15 days of age and farm records showed that feed intake at 14 days of age was only 11 bags (440 kg), compared to the standard intake of 14 bags (560 kg), a 21.4% decrease compared to normal feed intake. The chickens' feces were watery, and there were sporadic deaths, with 5-8 deaths per day. Dissections of weak, diseased chickens with poor spirits revealed fused, poorly demarcated glandular stomach papillae, black muscular keratin with ulcers and a bark-like appearance, thinning of the intestinal wall, and varying degrees of hemorrhagic inflammation. Based on the onset of the disease and the lesions found during the autopsy, the chickens were diagnosed with glandular myogastritis.

[0113] 4.2 Experimental methods and efficacy evaluation indicators

[0114] The chicken house is divided into three columns: left, middle and right, with about 2,800 chickens on each net. They are divided into three groups according to the position of the chickens in the chicken house, namely Group A, Group B and Group C from left to right. Since the specific number cannot be counted, each group is calculated as 2,800. Group A adds 1.25ml of the composition 1 in Example 1 to every 1L of water, and drinks it freely for 4 consecutive days. Group B adds 1.25ml of the composition 2 in Example 1 to every 1L of water, and drinks it freely for 4 consecutive days. Group C does not use any medicine and is free to eat and drink water. During the treatment period, the status of each group of chickens is observed every day, and the feed intake is counted, and the observation is continued until the end of 2 days of medication. After the end of medication, 2 thin chickens are picked in each group for autopsy to observe the changes in the glandular stomach.

[0115] 4.3 Test results

[0116] Table 3 shows the feed intake statistics for each group of chickens. Group C, which did not receive the drug, had a slight decrease in feed intake and undigested feed in its feces. However, on the third day after treatment with the drug of the present invention, the feed intake of Groups A and B significantly increased, and their feces improved somewhat. By the fourth day, feed intake had returned to normal. Dissections of selected lean chickens revealed significantly reduced symptoms in the glandular stomach and gizzard of Groups A and B, while Group C showed no significant difference from pre-experimental levels.

[0117] Table 5 Feed intake of chickens during treatment

[0118]

[0119]

[0120] Observation results show that the use of the drug provided by the present invention can quickly restore the decreased feed intake caused by chicken adenomyosis, improve the fecal status of the chickens, and has a significant therapeutic effect on chicken adenomyosis.

[0121] Example 5 Application of the composition of the present invention in the prevention of adenomyotic gastritis

[0122] 5.1 Test method

[0123] A farm in Dali Village, Qingxiu District, Nanning City, Guangxi Zhuang Autonomous Region, has a total of 2 chicken houses, each of which can raise 10,000 chickens. It mainly breeds Tuhuang No. 2 broilers, which are local fine broilers with a breeding cycle of 85-95 days and a market weight of 3.8-3.9 kilograms. Each batch of chickens on this farm will develop glandular gastritis at the age of 10-25 days. The chickens will have diarrhea, and there will be undigested feed in the feces. The chickens' feed intake is not up to standard, the chickens grow slowly, and their weight is light when they are marketed. On June 5, 2021, a new batch of chicks was introduced. Based on the previous incidence, 10,000 chickens in the first chicken house were selected for the experiment to prevent the occurrence of glandular gastritis in chickens. Method of use: Starting from 10 days of age, add 1.0 ml of Composition 1 in Example 1 to every 1 L of water, drink freely, and use it for 7 consecutive days. No medication is used for prevention in the second chicken house. Both chicken houses are operated according to the same feeding management and immunization procedures. The feed intake of each chicken house was counted, and the slaughter rate, slaughter weight, and feed-to-weight ratio of the chickens in the two chicken houses were calculated based on their weight when the chickens were slaughtered.

[0124] 5.2. Test results

[0125] After the first chicken house was treated with the drug of the present invention, feed intake gradually increased, with no decrease in feed intake between days 10 and 25. Autopsies of the individual dead chickens showed no symptoms of proventriculus, such as enlarged proventriculus, bleeding, or ulcers. The second chicken house, which did not receive any preventive medication, began to experience a decrease in feed intake at day 17, with watery stools and sporadic deaths. Autopsies revealed enlarged proventriculus, thickened stomach walls, fusion and disappearance of papillae in some chickens, and ulcers in the gizzard. Based on the clinical manifestations and anatomical changes, the chickens were diagnosed with proventriculus. At day 23, they were treated according to the farm's standard proventriculus treatment protocol. After a week, feed intake gradually increased, but never returned to normal levels.

[0126] The chickens in both chicken houses were slaughtered at the age of 85 days. The slaughter rate of the first chicken house was 98.5%, the average slaughter weight was 1.95kg, the feed consumption in the whole breeding cycle was 6.30kg, and the feed-to-meat ratio was 3.23; the slaughter rate of the second chicken house was 95.5%, the average slaughter weight was 1.85kg, the feed consumption in the whole breeding cycle was 6.47kg, and the feed-to-meat ratio was 3.50.

[0127] The test results show that for chicken farms with frequent glandular gastritis, using the drug of the present invention for prevention before the onset of the disease for 7 consecutive days can avoid the occurrence of the disease, increase the chicken yield, increase the slaughter weight, reduce the feed-to-weight ratio, improve the feed conversion rate, and increase economic benefits.

[0128] The above test results show that the Chinese medicine composition of the present invention can prevent the occurrence of the disease, increase the market weight, reduce the feed-to-weight ratio, improve the feed conversion rate, and increase the economic benefits of breeding when used in chickens before the high-incidence age of glandular gastritis and continuously used for 7 days at the recommended dose.

[0129] Note that the above are only preferred embodiments of the present application and the technical principles employed. Those skilled in the art will understand that the present application is not limited to the specific embodiments described herein, and that various obvious changes, readjustments, and substitutions can be made by those skilled in the art without departing from the scope of protection of the present application. Therefore, although the present application has been described in more detail through the above embodiments, the present application is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the technical concept of the present application, all of which fall within the scope of protection of the present application.

Claims

1. A Chinese medicine composition for preventing and / or treating chicken adenomyotic gastritis, characterized in that: The traditional Chinese medicine composition consists of the following components: 4-6 parts of Sophora flavescens, 2-4 parts of Patchouli, 3-4 parts of Rhubarb, 2-4 parts of Astragalus, 2-4 parts of Crataegus pinnatifida, and 1-3 parts of Areca catechu.

2. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition consists of the following components by weight: 4 parts of Sophora flavescens, 2 parts of Patchouli, 3 parts of Rhubarb, 4 parts of Astragalus, 4 parts of Charred Crataegus, and 3 parts of Areca.

3. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition consists of the following components by weight: 5 parts of Sophora flavescens, 3 parts of Patchouli, 4 parts of Rhubarb, 3 parts of Astragalus, 3 parts of Charred Crataegus, and 2 parts of Areca.

4. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition consists of the following components by weight: 6 parts of Sophora flavescens, 4 parts of Patchouli, 3 parts of Rhubarb, 2 parts of Astragalus, 2 parts of Charred Crataegus, and 1 part of Areca catechu.

5. The Chinese medicine composition according to any one of claims 1 to 4, characterized in that The Chinese medicine composition is prepared into a preparation suitable for oral administration; The oral preparation is a liquid preparation suitable for oral administration.

6. A method for preparing the Chinese medicine composition according to any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: (a) Soak Patchouli in water and then steam distill to extract the volatile oil; (b) boiling Sophora flavescens, Rhubarb, Astragalus, Charred Crataegus, and Areca catechu with water and collecting the filtrate; (c) mixing the volatile oil in (a) and the filtrate in (b) to obtain the traditional Chinese medicine composition.

7. The preparation method according to claim 6, characterized in that In (a), the amount of water used to soak Patchouli is 8 to 12 times the weight of Patchouli, the soaking time is 0.5 to 1.5 h, and the extraction time is 7 to 10 h.

8. The preparation method according to claim 6, characterized in that (b) The amount of water added is 8 to 12 times the total weight of Sophora flavescens, Rhubarb, Astragalus, Charred Hawthorn and Areca catechu, and the decoction is performed 2 to 4 times for 1.5 to 2.5 hours.

9. The preparation method according to any one of claims 6 to 8, characterized in that In (c), the filtrate is first concentrated to a relative density of 1.05-1.1 at 50-60° C., and then the volatile oil in (a) is added and mixed to obtain the traditional Chinese medicine composition.

10. A Chinese medicine preparation, characterized in that The traditional Chinese medicine preparation is prepared from the traditional Chinese medicine composition according to any one of claims 1 to 4, and the dosage form of the traditional Chinese medicine preparation includes solid, semi-solid or liquid dosage forms.

11. Use of the traditional Chinese medicine composition according to any one of claims 1 to 5 or the traditional Chinese medicine preparation according to claim 10 in the preparation of a medicament for preventing and / or treating chicken adenomyotic gastritis; The medicine is used by mixing with drink; The therapeutic dosage of the drug is 1.0-1.25 ml per liter of water, for 5-7 consecutive days; The preventive dosage of the drug is 0.8-1.2 ml per liter of water, for 7-10 consecutive days.

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