Anaerobic granular sludge inoculants, their preparation methods and applications

Abstract

The application belongs to the technical field of wastewater COD treatment, and particularly relates to an anaerobic granular sludge bacterium agent, a preparation method and application thereof. The anaerobic granular sludge bacterium agent comprises the following raw materials in parts by mass: anaerobic granular sludge: 1-3 parts, a composite enzyme agent: 1-3 parts, a nutrient agent: 1-3 parts, and freeze-dried powder: 1-3 parts. The application is convenient to transport and use, has high activity, and greatly reduces the wastewater treatment cost.

Description

Technical Field

[0001] This invention belongs to the field of wastewater COD treatment technology, specifically relating to anaerobic granular sludge bacteria agents, their preparation methods, and applications. Background Technology

[0002] With industrial development, wastewater discharge has been increasing year by year, bringing with it the challenge of wastewater treatment. Organic wastewater, in particular, presents significant treatment difficulties. Current technologies primarily treat organic wastewater using anaerobic granular sludge, which needs to be replaced after its service life. Firstly, the cost of anaerobic granular sludge for large quantities of organic wastewater is prohibitively high, reaching thousands of yuan per ton. Secondly, the wastewater treatment industry currently transports anaerobic granular sludge via tanker trucks, resulting in high transportation costs. Furthermore, transportation limitations often lead to the death and loss of a large number of anaerobic bacteria within the sludge. A complete anaerobic treatment tower requires 2000 tons of granular sludge; however, transportation limitations make replenishment inconvenient, and the replenished anaerobic granular sludge has low activity, resulting in substantial cost losses. Summary of the Invention

[0003] To address the aforementioned technical problems, this invention provides an anaerobic granular sludge inoculant and its preparation method, which is convenient to transport and use, exhibits high activity, and significantly reduces wastewater treatment costs. This invention also provides the preparation method and its applications.

[0004] The anaerobic granular sludge agent of the present invention comprises the following raw materials in parts by weight:

[0005] Anaerobic granular sludge: 1-3 parts

[0006] Compound enzyme preparation: 1-3 parts,

[0007] Nutrient supplement: 1-3 servings

[0008] Freeze-dried powder: 1-3 parts.

[0009] Preferably, the anaerobic granular sludge has a solid content of 10%, a particle size of 1-3 mm, and a VSS / TSS ratio of >65%.

[0010] Preferably, the compound enzyme is one or more of amylase, protease, cellulase, pectinase and glucanase, with an enzyme activity of not less than 5000 u / g.

[0011] Preferably, the nutrient includes one or more of beef extract, peptone, urea, threonine, and glucose.

[0012] Preferably, the freeze-dried powder includes mannitol, oligopeptides, polypeptides, and collagen.

[0013] The preparation method of the anaerobic granular sludge bacterial agent of the present invention includes the following steps:

[0014] (1) Mix anaerobic granular sludge, nutrients, compound enzymes and freeze-dried powder, adjust the pH to 6-10, keep the temperature at 20-45℃, and culture for 10-12 hours to obtain anaerobic granular sludge culture mixture.

[0015] (2) Centrifuge the anaerobic granular sludge culture mixture, remove the supernatant, add a protective agent, and obtain an anaerobic granular sludge sample;

[0016] (3) The anaerobic granular sludge sample is sieved through a fiber sieve to obtain the anaerobic granular sludge sieved material.

[0017] (4) Place the anaerobic granular sludge sieved material in a refrigerated dryer for freeze drying to obtain the bacterial agent.

[0018] Preferably, the protective agent is diatomaceous earth, and the mass ratio of the protective agent to the anaerobic granular sludge culture mixture is (1-5):1.

[0019] Preferably, in step (2), the centrifuge speed is 4000-8000 rpm and the centrifugation time is 3-6 min.

[0020] Preferably, the fiber screen is 20-80 mesh.

[0021] Preferably, in step (4), the freeze-drying conditions are: temperature -10℃ to -50℃, freezing time 8 to 16 hours, and drying time 14 to 16 hours.

[0022] The application of the anaerobic granular sludge bacterial agent of the present invention involves mixing the bacterial agent with water at a mass ratio of (1-2):(2-4), activating and culturing it at 25-30°C for 24-48 hours, and then using the activated anaerobic granular sludge to treat organic wastewater.

[0023] Compared with the prior art, the beneficial effects of the present invention are:

[0024] 1. The anaerobic granular sludge inoculant of the present invention is easy to use. It only requires the addition of water and short-term activation culture to meet the start-up requirements of the anaerobic tower. This avoids the need for a large amount of sludge and water and a long acclimatization culture of the anaerobic granular sludge required by conventional methods when starting the anaerobic tower, thus shortening the culture time and making it easier to store.

[0025] 2. It reduces transportation costs, time costs, and the cost of anaerobic granular sludge. At the same time, the product can maintain high anaerobic sludge activity for a short period of time, making it convenient for wastewater treatment plants to quickly start up and restore anaerobic treatment systems. Detailed Implementation

[0026] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments.

[0027] Unless otherwise specified, all raw materials used in the examples were commercially available.

[0028] Example 1

[0029] The anaerobic granular sludge agent of the present invention comprises the following raw materials in parts by weight:

[0030] Anaerobic granular sludge: 1 part

[0031] Compound enzyme preparation: 3 parts

[0032] Nutritional supplement: 2 servings.

[0033] Freeze-dried powder: 1 part.

[0034] The anaerobic granular sludge has a solids content of 10%, a particle size of 1-3 mm, and a VSS / TSS ratio of >65%.

[0035] The compound enzyme is composed of amylase, protease, cellulase, pectinase and glucanase in a mass ratio of 3:2:1:5, with an enzyme activity of not less than 5000 u / g.

[0036] The nutrient solution consists of beef extract, peptone, urea, threonine, and glucose in a mass ratio of 3:3:0.5:0.5:3.

[0037] The freeze-dried powder contains mannitol, oligopeptides, polypeptides, and collagen in a mass ratio of 1:2:4:3.

[0038] The preparation method of the anaerobic granular sludge bacterial agent of the present invention includes the following steps:

[0039] (1) Mix anaerobic granular sludge, nutrients, compound enzymes and freeze-dried powder, adjust the pH to 6, and culture at 45℃ for 12 hours to obtain anaerobic granular sludge culture mixture.

[0040] (2) Centrifuge the anaerobic granular sludge culture mixture, remove the supernatant, add a protective agent, and obtain an anaerobic granular sludge sample;

[0041] (3) The anaerobic granular sludge sample is sieved through a fiber sieve to obtain the anaerobic granular sludge sieved material.

[0042] (4) Place the anaerobic granular sludge sieved material in a refrigerated dryer for freeze drying to obtain the bacterial agent.

[0043] The protective agent is diatomaceous earth, and the mass ratio of the protective agent to the anaerobic granular sludge culture mixture is 1:1.

[0044] In step (2), the centrifuge speed is 6000 rpm / min, and the centrifugation time is 5 min.

[0045] The fiber screen has a mesh size of 20.

[0046] In step (4), the freeze-drying conditions are: temperature -10℃, freezing time 16h, and drying time 15h.

[0047] Example 2

[0048] The anaerobic granular sludge agent of the present invention comprises the following raw materials in parts by weight:

[0049] Anaerobic granular sludge: 2 parts

[0050] Compound enzyme preparation: 1 part

[0051] Nutritional supplement: 3 servings.

[0052] Freeze-dried powder: 2 parts.

[0053] The anaerobic granular sludge has a solids content of 10%, a particle size of 1-3 mm, and a VSS / TSS ratio of >65%.

[0054] The compound enzyme is composed of amylase, protease, cellulase, pectinase and glucanase in a mass ratio of 3:3:1:4, with an enzyme activity of not less than 5000 u / g.

[0055] The nutrient solution consists of beef extract, peptone, urea, threonine, and glucose in a mass ratio of 3:3:0.5:0.5:3.

[0056] The freeze-dried powder contains mannitol, oligopeptides, polypeptides and collagen in a mass ratio of 1:3:3:3.

[0057] The preparation method of the anaerobic granular sludge bacterial agent of the present invention includes the following steps:

[0058] (1) Mix anaerobic granular sludge, nutrients, compound enzymes and freeze-dried powder, adjust the pH to 10, set the temperature to 30℃, and culture for 12h to obtain anaerobic granular sludge culture mixture.

[0059] (2) Centrifuge the anaerobic granular sludge culture mixture, remove the supernatant, add a protective agent, and obtain an anaerobic granular sludge sample;

[0060] (3) The anaerobic granular sludge sample is sieved through a fiber sieve to obtain the anaerobic granular sludge sieved material.

[0061] (4) Place the anaerobic granular sludge sieved material in a refrigerated dryer for freeze drying to obtain the bacterial agent.

[0062] The protective agent is diatomaceous earth, and the mass ratio of the protective agent to the anaerobic granular sludge culture mixture is 3:1.

[0063] In step (2), the centrifuge speed is 6000 rpm / min, and the centrifugation time is 5 min.

[0064] The fiber sieve has a mesh size of 50.

[0065] In step (4), the freeze-drying conditions are: temperature -30℃, freezing time 12h, and drying time 14h.

[0066] Example 3

[0067] The anaerobic granular sludge agent of the present invention comprises the following raw materials in parts by weight:

[0068] Anaerobic granular sludge: 3 parts

[0069] Compound enzyme preparation: 2 parts,

[0070] Nutritional supplement: 1 serving.

[0071] Freeze-dried powder: 3 parts.

[0072] The anaerobic granular sludge has a solids content of 10%, a particle size of 1-3 mm, and a VSS / TSS ratio of >65%.

[0073] The compound enzyme is composed of amylase, protease, cellulase, pectinase and glucanase in a mass ratio of 2:2:2:5, with an enzyme activity of not less than 5000 u / g.

[0074] The nutrient solution consists of beef extract, peptone, urea, threonine, and glucose in a mass ratio of 3:3:0.5:0.5:3.

[0075] The freeze-dried powder contains mannitol, oligopeptides, polypeptides, and collagen in a mass ratio of 1:2:4:3.

[0076] The preparation method of the anaerobic granular sludge bacterial agent of the present invention includes the following steps:

[0077] (1) Mix anaerobic granular sludge, nutrients, compound enzymes and freeze-dried powder, adjust the pH to 8, set the temperature to 20℃, and culture for 12h to obtain anaerobic granular sludge culture mixture.

[0078] (2) Centrifuge the anaerobic granular sludge culture mixture, remove the supernatant, add a protective agent, and obtain an anaerobic granular sludge sample;

[0079] (3) The anaerobic granular sludge sample is sieved through a fiber sieve to obtain the anaerobic granular sludge sieved material.

[0080] (4) Place the anaerobic granular sludge sieved material in a refrigerated dryer for freeze drying to obtain the bacterial agent.

[0081] The protective agent is diatomaceous earth, and the mass ratio of the protective agent to the anaerobic granular sludge culture mixture is 5:1.

[0082] In step (2), the centrifuge speed is 6000 rpm / min, and the centrifugation time is 5 min.

[0083] The fiber screen has an 80-mesh aperture.

[0084] In step (4), the freeze-drying conditions are: temperature -50℃, freezing time 8h, and drying time 16h.

[0085] Performance testing

[0086] The anaerobic granular sludge inoculant of the present invention is prepared by mixing the inoculant with water at a mass ratio of 1:2, activating and culturing at 25°C for 48 hours to obtain activated anaerobic granular sludge, and then conducting performance tests according to T / CBFIA 14001-2017. The results are shown in Table 1.

[0087] Table 1 Performance Test Table for Examples 1-3

[0088]

[0089] As can be seen from Table 1, the activated anaerobic granular sludge obtained by this invention fully meets the performance standards of T / CBFIA 14001-2017 and can be used to treat organic wastewater.

Claims

1. An anaerobic granular sludge bacterin characterized in that, The raw materials include the following mass fractions: Anaerobic granular sludge: 1-3 parts, Compound enzyme agent: 1-3 parts, Nutrient agent: 1-3 parts, Freeze-dried powder: 1-3 parts; The compound enzyme agent is one or more of amylase, protease, cellulase, pectinase and glucanase; The nutrient agent is one or more of beef extract, peptone, urea, threonine and glucose; The freeze-dried powder is mannitol, oligopeptide, polypeptide and collagen.

2. The anaerobic granular sludge bacterin of claim 1, wherein, The anaerobic granular sludge has a solid content of 10%, a particle size of 1-3 mm and a VSS / TSS of greater than 65%.

3. The anaerobic granular sludge bacterin of claim 1, wherein, The enzyme activity of the compound enzyme agent is not less than 5000 u / g.

4. A method for producing the anaerobic granular sludge bacterin according to any one of claims 1 to 3, characterized by, The method comprises the following steps: (1) mixing the anaerobic granular sludge, the nutrient agent, the compound enzyme agent and the freeze-dried powder, adjusting the pH value to 6-10, the temperature to 20-45℃, and culturing for 10-12 h to obtain an anaerobic granular sludge culture mixture; (2) centrifuging the anaerobic granular sludge culture mixture, removing the supernatant, adding a protective agent, and obtaining an anaerobic granular sludge sample; (3) sieving the anaerobic granular sludge sample through a fiber screen to obtain an anaerobic granular sludge sieved product; (4) freeze-drying the anaerobic granular sludge sieved product in a freeze dryer to obtain the microbial agent; The protective agent is diatomite.

5. The method of claim 4, wherein the oxygen granular sludge bacterin is prepared by, The mass ratio of the protective agent to the anaerobic granular sludge culture mixture is (1-5):

1.

6. The method of claim 4, wherein the oxygen granular sludge bacterin is prepared by the steps of: In step (2), the centrifuge rotates at a speed of 4000-8000 rpm for 3-6 min.

7. The method of claim 4, wherein the oxygen granular sludge bacterin is prepared by the steps of: The fiber screen has a mesh size of 20-80.

8. The method for preparing the oxygen granular sludge bacterial agent according to claim 4, characterized in that, In step (4), the freeze-drying conditions are as follows: temperature -10℃ to -50℃, freezing time 8-16 h and drying time 14-16 h.

9. Use of the anaerobic granular sludge bacterin according to any one of claims 1 to 3, characterized in that, The microbial agent is mixed with water at a mass ratio of (1-2):(2-4), activated and cultured at 25-30℃ for 24-48 h, and then used for treating organic wastewater.

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