Skin tightening and anti-wrinkle essence and preparation method thereof

Abstract

The present invention proposes a skin tightening and anti-wrinkle essence and a preparation method thereof, and belongs to the field of cosmetic technology. It is prepared by the following raw materials by weight: 7-10 parts of active extracts, 5-7 parts of polypeptide compositions, 0.5-2 parts of penetration enhancer compositions, 0.1-0.5 parts of DNA sodium, 0.3-0.5 parts of soluble collagen, 5-10 parts of hydroxypropyl tetrahydropyrantriol, 2-8 parts of emollients, 5-10 parts of moisturizers, 0.5-2 parts of emulsifiers, 0.2-0.5 parts of thickeners, 0.1-0.5 parts of hirudin, 0.02-0.05 parts of disodium EDTA, and 50-70 parts of deionized water. The skin tightening and anti-wrinkle essence prepared by the present invention has the effects of whitening, anti-oxidation, anti-aging, anti-photoaging, tightening skin, improving skin elasticity, and fading skin wrinkles, and the multi-component synergistic effect is more obvious, and the cost is low, the preparation method is simple, and it has broad application prospects.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a skin tightening and anti-wrinkle essence and a preparation method thereof. Background Art

[0002] The biological aging process is the process of cells drying up and dying. Like other cells, skin cells are mostly composed of water. When there is enough water, skin cells will be full of vitality and have a strong ability to resist harsh environments and repair themselves. In today's society, the air environment is seriously polluted. Various bacteria, viruses, radiation, and haze make the skin lose moisture faster, and the skin ages faster. Dry, tight, rough, flaky, loss of elasticity, and large pores are all manifestations of skin dehydration, and they all need to be replenished immediately. The moisture of the skin mainly comes from the body. With the increase of age and changes in the external environment, the protective effect of the sebum membrane will weaken or be damaged, and the natural moisturizing factor of the stratum corneum will also be lost. At this time, the stratum corneum of the skin is prone to dehydration, which can easily lead to poor keratin metabolism, impurities and metabolites in the cells cannot be removed in time, and the so-called "thick keratin" phenomenon will occur, which will affect the skin color and make the skin look dull and dull, and will also hinder the penetration of skin care products. Even if whitening care is performed, the effect is limited.

[0003] Due to the fast pace of modern life and various environmental influences, the skin is often dry and dull, and cosmetics are becoming a daily necessity and widely used by people. Essence water is considered a necessity for basic skin care by most women because of its high nutritional content, low irritation to the skin, and ability to regulate skin cell activity.

[0004] Chinese invention patent application CN111150701A discloses an anti-wrinkle and firming essence and a preparation method thereof. The anti-wrinkle and firming composition in the patent comprises, by weight: 0.5-3 parts of Spirulina maxima extract, 0.005-0.1 parts of sodium hyaluronate, 0.01-0.05 parts of cyclodextrin, 0.01-0.05 parts of ubiquinone, 0.1-1 parts of oat alkaloids, 1-5 parts of yeast extract, 0.1-1 parts of fucoidan, 0.01-0.1 parts of humic acid, 0.1-3 parts of Hylocereus occidentalis fruit extract, 0.05-0.3 parts of decapeptide-40, 0.05-0.3 parts of glycoprotein, and 0.005-0.05 parts of rose oil. It can quickly penetrate deep into the skin to replenish moisture, smooth the skin, eliminate fine lines and wrinkles, give the skin elasticity and firmness, delay skin aging, promote the regeneration of young cells, and make the skin appear bright and lustrous. However, this invention directly extracts from natural organisms and obtains small molecule polypeptides through protease hydrolysis, which are prone to problems such as poor stability and poor anti-wrinkle effect. Summary of the invention

[0005] The purpose of the present invention is to provide a skin tightening and anti-wrinkle essence and a preparation method thereof, which has the effects of whitening, anti-oxidation, anti-aging, anti-photoaging, skin tightening, improving skin elasticity, and reducing skin wrinkles. The multi-component synergistic effect has more obvious effects, and the cost is low. The preparation method is simple and has broad application prospects.

[0006] The technical solution of the present invention is achieved in this way:

[0007] The invention provides a skin tightening and anti-wrinkle essence, which is prepared from the following raw materials in parts by weight: 7-10 parts of active extracts, 5-7 parts of polypeptide compositions, 0.5-2 parts of penetration enhancer compositions, 0.1-0.5 parts of sodium DNA, 0.3-0.5 parts of soluble collagen, 5-10 parts of hydroxypropyl tetrahydropyrantriol, 2-8 parts of emollients, 5-10 parts of humectants, 0.5-2 parts of emulsifiers, 0.2-0.5 parts of thickeners, 0.1-0.5 parts of hirudin, 0.02-0.05 parts of disodium EDTA and 50-70 parts of deionized water.

[0008] As a further improvement of the present invention, the penetration enhancer composition comprises ethoxydiglycol and a penetration enhancing compound in a mass ratio of 4-7:3, and the structural formula of the penetration enhancing compound is shown in Formula I:

[0009]

[0010] Wherein, R=C6-C18 alkyl chain.

[0011] As a further improvement of the present invention, the synthesis method of the penetration-enhancing compound is as follows: dissolving a dihaloalkane in dichloromethane, adding a dichloromethane solution of caprolactam and triethylamine dropwise, heating to reflux, stirring to react for a first period of time, then adding a dichloromethane solution of menthol dropwise, heating to reflux, stirring to react for a second period of time, removing the solvent under reduced pressure, separating and purifying by column chromatography, and obtaining the penetration-enhancing compound.

[0012] As a further improvement of the present invention, the molar ratio of the dihaloalkane, caprolactam, menthol and triethylamine is 1:0.9-1:0.9-1:4-6, the first time period is 3-5 hours, and the second time period is 4-6 hours.

[0013] As a further improvement of the present invention, the polypeptide composition is a composition of palmitoyl tripeptide-5, hexapeptide-11, hexapeptide-9, acetyl hexapeptide-8, and arginine / lysine polypeptides, with a mass ratio of 4-6:1-3:2-4:0.5-1:3-5.

[0014] As a further improvement of the present invention, the preparation method of the active extract is as follows:

[0015] S1. Supercritical fluid extraction: The Bidens pilosa and Dendrobium candidum were dried, crushed, and subjected to supercritical fluid extraction to obtain an oil extract, and the remaining material was retained;

[0016] S2. deep eutectic solvent enzymatic hydrolysis: adding the remaining material in step S1 to an aqueous solution containing a deep eutectic solvent, adding a composite enzyme for enzymatic hydrolysis, inactivating the enzyme, and obtaining an enzymatic hydrolysis product;

[0017] S3 fermentation: the enzymatic product obtained in step S2 was mixed with water, sterilized, inoculated with long Bifidobacterium and breve Bifidobacterium species seed solution, fermented, filtered, washed with solids, the eluate was collected and concentrated to obtain a bacterial solution, and the filtrate was retained;

[0018] S4. Alcohol precipitation: adding ethanol to the filtrate in step S3, centrifuging, washing, drying, to obtain a polysaccharide composition, recovering ethanol from the supernatant, and retaining the remaining liquid;

[0019] S5. Purification by macroporous resin: The solvent of the remaining liquid in step S4 is removed under reduced pressure, and the filtrate is purified by macroporous resin, and the solvent is removed under reduced pressure, and the sample is diluted with 65-75wt% ethanol, and the column is wet loaded, and the column volume is 45-55mL, and the eluate with 65-75wt% ethanol concentration is collected, and the ethanol is removed under reduced pressure and dried to obtain an active mixture;

[0020] S6. Preparation of bifid yeast lysate: freezing the bacterial solution obtained in step S3 with liquid nitrogen, thawing it at room temperature, centrifuging it, collecting the supernatant, and freeze-drying it to obtain bifid yeast lysate;

[0021] S7. Preparation of active extract: the oil extract prepared in step S1, the polysaccharide composition prepared in step S4, the active mixture prepared in step S5, the bifida yeast lysate prepared in step S6 and vitamin C are uniformly mixed to prepare the active extract.

[0022] As a further improvement of the present invention, the mass ratio of Bidens pilosa and Dendrobium officinale in step S1 is 7-10:3-5, and the conditions of the supercritical fluid extraction are pressure 27-35 MPa, temperature 32-37° C., and time 70-90 min; the mass ratio of the residual material and the aqueous solution containing the deep eutectic solvent in step S2 is 10:35-60, the water content in the aqueous solution containing the deep eutectic solvent is 40-60wt%, and the deep eutectic solvent is composed of choline chloride and glucose in a molar ratio. The composite enzyme is prepared by mixing the mixture in a ratio of 1:1, wherein the composite enzyme is composed of a mixture of cellulase and pectinase in a mass ratio of 10-15:3-5, the addition amount of the composite enzyme is 1-2wt% of the total mass of the system, the temperature of the enzymolysis is 40-50°C, and the time is 2-4h; the mass ratio of the enzymolysis product to water in step S3 is 1:1.5-3, the inoculation amounts of the long bifidobacterium and the breve bifidobacterium seed liquid are 2-4v / v% and 1-3v / v%, respectively, and the bacterial content of the seed liquid is 10 8 -10 9 cfu / mL, and the fermentation culture conditions are anaerobic conditions, 36-39°C, 150-200r / min, and the fermentation culture is 24-48h.

[0023] As a further improvement of the present invention, in step S4, ethanol is added to the system ethanol content of 60-70wt%, and the precipitation time is 3-5h; in step S5, the macroporous resin is selected from at least one of XDA-2, NKA-9, SP-825, D101, HPD600, X-5, LSA-10, NKI-9 and AB-8, and is diluted with 65-75wt% ethanol to a concentration of 0.8-1.2mg / mL; in step S7, the mass ratio of the oil extract, the polysaccharide composition, the active mixture, the bifida yeast lysate and the vitamin C is 5-7:12-15:10-17:3-5:1-2.

[0024] As a further improvement of the present invention, the emollient is selected from at least one of tri(ethylhexanoin)glyceryl, isononyl isononanoate, polydimethylsiloxane, caprylic / capric triglyceride, and avocado tree (BUTYROSPERMUM PARKII) fruit fat; the humectant is selected from at least one of glycerol, butylene glycol, propylene glycol, hexylene glycol, sodium hyaluronate, betaine, and trehalose; the emulsifier is selected from at least one of glyceryl stearate, PEG-100 stearate, cetearyl olive oil ester, sorbitan olive oil ester, C12-20 alkyl glucoside, and polyglyceryl-2 stearate; the thickener is selected from at least one of carbomer, acrylic acid (ester) / C10-30 alcohol acrylate crosspolymer, sodium polyacryloyldimethyl taurate, and xanthan gum.

[0025] The present invention further protects a method for preparing the above-mentioned skin tightening and anti-wrinkle essence, comprising the following steps:

[0026] (1) Add the active extract, polypeptide composition, soluble collagen, DNA sodium, hydroxypropyl tetrahydropyrantriol, hirudin, moisturizer, thickener, and disodium EDTA into deionized water, heat to 70-80°C, and stir at 300-500 r / min for 10-20 min to prepare phase A.

[0027] (2) adding the penetration enhancer composition to the emollient and emulsifier, heating to 70-80° C., and stirring at 300-500 r / min for 10-20 min to obtain phase B;

[0028] (3) Add phase B into phase A, stir at 300-500 r / min for 5-15 min, emulsify at 7000-10000 r / min for 10-20 min, keep warm for 10-20 min, cool down, discharge, and obtain a firming skin anti-wrinkle essence.

[0029] The present invention has the following beneficial effects:

[0030] The present invention prepares a penetration-promoting compound, which is connected by menthol and caprolactam through an alkyl chain, thereby overcoming the problem that menthol is easy to volatilize due to its small molecular weight and thus reduces its effectiveness. At the same time, the preparation method is simple, the reaction conditions are mild, and the yield is high. The prepared penetration-promoting compound has good effects of promoting active substances to penetrate into the skin, and exerts better firming, improving skin elasticity, and anti-wrinkle effects. Among them, the caprolactam part can interact with the stratum corneum lipid bilayer to increase its fluidity; the menthol part can destroy the conformational order of the stratum corneum lipid bilayer; and reduce the phase transition temperature of the stratum corneum lipid. The present invention mixes the prepared penetration-promoting compound with ethoxydiglycol for use, which has a synergistic effect.

[0031] Sodium DNA is added to the skin tightening and anti-wrinkle essence of the present invention, and its DNA nucleotide fragments have a significant effect on increasing the basic values ​​of moisture retention, elasticity and skin thickness and reducing skin wrinkles. At the same time, sodium DNA stimulates cell repair, alleviates inflammatory symptoms, accelerates the healing of skin microlesions, has a regeneration and photoprotective effect on keratinocytes and fibroblasts, and has a protective effect on ultraviolet-induced fibroblast damage.

[0032] Hydroxypropyl tetrahydropyrantriol is the core ingredient in Pro-XylaneTM. Hydroxypropyl tetrahydropyrantriol can promote the production of glycosaminoglycans (GAGs) and proteoglycans (PGs) in the skin.

[0033] The peptide composition includes palmitoyl tripeptide-5, hexapeptide-11, hexapeptide-9, acetyl hexapeptide-8, and arginine / lysine peptides. Palmitoyl tripeptide-5 can stimulate the synthesis of collagen and glycosaminoglycans, enhance skin firmness and elasticity, and play an anti-wrinkle and anti-aging role; hexapeptide-11 can improve water retention, reduce wrinkles, fight aging, and return the skin to a soft and firm state; hexapeptide-9 can increase the synthesis of type I collagen, type IV collagen, laminin-5, and integrin, promote epidermal cell differentiation and maturation and skin regeneration, and reduce wrinkles; acetyl hexapeptide-8 has the effects of scavenging free radicals, promoting collagen synthesis, moisturizing, and reducing fine lines and wrinkles; arginine / lysine peptides can enhance skin defense function, improve skin sensitivity, and regulate the water and oil balance of the skin. Several protein peptides work synergistically to achieve good skin tightening, anti-wrinkle, moisturizing, and improving skin elasticity.

[0034] The raw materials for preparing the active extract of the present invention are Bidens pilosa L. extract and Dendrobium candidum. The oil extract extracted by supercritical carbon dioxide extraction technology has unique pharmacological properties, such as antibacterial, anti-inflammatory and anti-allergic, detoxifying and detumescence, clearing away heat and relieving pain, promoting blood circulation and dispersing blood stasis, regulating qi and eliminating accumulation and the like.

[0035] The active ingredients in the extract are the key to its efficacy. Traditional extraction processes have the disadvantages of complex procedures, low efficiency, and poor extraction quality. The present invention uses enzymes as catalysts to hydrolyze plant cell walls and promote the diffusion of active ingredients from the cell to the extraction medium. However, due to the poor solubility and easy inactivation of biological enzymes in traditional organic solvents, the present invention uses deep eutectic solvents as reaction media. On the one hand, it can greatly improve the extraction of compounds that are difficult to dissolve in water. On the other hand, it has many advantages such as low toxicity, green, dynamically adjustable, non-flammable, and strong solubility for compounds. Glucose is used as one of the raw materials in the deep eutectic solvent of the present invention, which also provides a carbon source for subsequent fermentation.

[0036] The present invention uses bifidobacteria for fermentation. The fermented products of bifidobacteria are rich in vitamins, amino acids and minerals that nourish cells, which can accelerate skin metabolism and repair damaged skin. At the same time, the dissolution of active components in the raw materials can be improved, so that the fermented products are rich in flavonoids, organic acids, terpenoids, polysaccharides and other substances.

[0037] The polysaccharide composition obtained by alcohol precipitation of the fermentation product has excellent moisturizing and antioxidant properties. After separation and purification by macroporous resin, the obtained active mixture is rich in flavonoids, such as isorhamnoside, apigenin, etc., which have strong antioxidant effects, can help the skin resist free radical damage and slow down aging. It contains phytanic acid, which can stimulate the activity of skin cells, promote the production and repair of collagen and elastin, thereby helping to improve the elasticity and firmness of the skin and make the skin younger and healthier; the diterpene alcohol contained is similar to the diterpene alcohol of A-ol, which can be converted into phytanic acid in the body and then bind to retinoic acid receptors and retinol X receptors, thereby regulating gene expression and protein production and promoting epidermal metabolism.

[0038] On the one hand, the fermentation process produces abundant fermentation by-products, and on the other hand, it also rapidly promotes the proliferation of bifidobacteria. After being frozen by liquid nitrogen, the bifidobacterium cell wall is broken and the contents are dissolved. The obtained bifidobacterium lysate can regulate the expression of the deacetylase-6 gene, which helps to prolong cell life, delay aging, and has whitening, anti-oxidation and anti-aging effects.

[0039] The skin tightening and anti-wrinkle essence prepared by the invention has the effects of whitening, anti-oxidation, anti-aging, anti-photoaging, skin tightening, improving skin elasticity, reducing skin wrinkles, etc., and the multi-components work synergistically, the effect is more obvious, and the cost is low. The preparation method is simple and has broad application prospects. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Cellulase, 11,000 U / g, pectinase, 60,000 U / g, were purchased from Xiasheng (Beijing) Biotechnology Development Co., Ltd. Bifidobacterium longum, 10 billion cfu / g, was purchased from Guangdong Hongyou Biotechnology Co., Ltd.; Bifidobacterium breve, 10 billion cfu / g, was purchased from Shaanxi Saien Biotechnology Co., Ltd.

[0042] Preparation method of bacterial seed liquid: inoculate the bacterial strain into Gao's medium, activate and culture at 37℃, 100r / min for 24h, and obtain a liquid containing 10 8 -10 9 cfu / mL of bacterial seed solution.

[0043] Preparation Example 1 Synthesis of penetration-enhancing compounds

[0044] Synthesis route:

[0045]

[0046] The method is as follows: 0.1 mol 1,12-dichlorododecane is dissolved in 200 mL dichloromethane, 50 mL dichloromethane solution containing 0.09 mol caprolactam and 0.5 mol triethylamine is added dropwise, heated to reflux, stirred to react for 4 hours, then 20 mL dichloromethane solution containing 0.09 mol menthol is added dropwise, heated to reflux, stirred to react for 5 hours, the solvent is removed under reduced pressure, and column chromatography (volume ratio of petroleum ether: ethyl acetate = 20:1) is used for separation and purification to obtain a permeation-enhancing compound with a yield of 82.5%. ESI-MS calculated value: C 28 H 54 NO2(M+H) + 436.41, measured value: 436.4.

[0047] NMR results: 1 H NMR (300MHz, CDCl3) δ3.37(t, 2H), 3.2(m, 4H), 2.78(m, 1H), 2.17(t, 2H), 1.80(m, 1H), 1.73(m, 1H), 16 0 (m, 1H), 1.55-1.59 (m, 8H), 1.45 (m, 2H), 1.40 (m, 4H), 1.27-1.30 (m, 18H), 1.04 (s, 3H), 1.01 (d, 6H).

[0048] Preparation Example 2 Preparation of active extract

[0049] Here’s how:

[0050] S1 supercritical flow extraction: 7 parts by weight of Bidens pilosa and 3 parts by weight of Dendrobium candidum were dried, crushed, and subjected to supercritical fluid extraction to obtain an oil extract, and the remaining material was retained;

[0051] The supercritical fluid extraction conditions are: pressure 27 MPa, temperature 32°C, time 70 min;

[0052] S2. deep eutectic solvent enzymatic hydrolysis: 10 parts by weight of the remaining material in step S1 was added to 35 parts by weight of an aqueous solution containing a deep eutectic solvent, and a composite enzyme was added, wherein the amount of the composite enzyme added was 1 wt % of the total mass of the system, and the enzymatic hydrolysis was performed at 40° C. for 2 h, and the enzyme was inactivated to obtain an enzymatic hydrolysis product;

[0053] The water content in the aqueous solution containing the deep eutectic solvent is 40 wt %, and the deep eutectic solvent is prepared by mixing choline chloride and glucose in a molar ratio of 1:1;

[0054] The complex enzyme is composed of a mixture of cellulase and pectinase, with a mass ratio of 10:3;

[0055] S3. Fermentation: 10 parts by weight of the enzymatic hydrolysate obtained in step S2 was mixed with 15 parts by weight of water, sterilized, and inoculated with a seed solution of Bifidobacterium longum and Bifidobacterium breve, wherein the inoculation amount of the seed solution of Bifidobacterium longum and Bifidobacterium breve was 2 v / v% and 1 v / v%, respectively, under anaerobic conditions, 36 ° C, 150 r / min, and the fermentation culture was carried out for 24 h, filtered, the solid was washed, and the eluate was collected and concentrated to obtain a bacterial solution, and the filtrate was retained;

[0056] S4. Alcohol precipitation: adding ethanol to the filtrate in step S3 to an ethanol content of 60 wt % in the system, precipitating for 3 h, centrifuging, washing, and drying to obtain a polysaccharide composition, recovering ethanol from the supernatant, and retaining the remaining liquid;

[0057] S5. Macroporous resin purification: The residual liquid in step S4 is decompressed to remove the solvent, and the filtrate is purified by macroporous resin NKA-9, and the solvent is removed by decompression, and the filtrate is diluted with 65wt% ethanol to a concentration of 0.8mg / mL, loaded, and wet packed into a column. The specification of the chromatography column is 15mm×500mm, and the column volume is 50mL. The eluate with a concentration of 65wt% ethanol is collected, the ethanol is removed under reduced pressure, and dried to obtain an active mixture;

[0058] S6. Preparation of bifid yeast lysate: freezing the bacterial solution obtained in step S3 with liquid nitrogen, thawing it at room temperature, centrifuging it, collecting the supernatant, and freeze-drying it to obtain bifid yeast lysate;

[0059] S7. Preparation of active extract: 5 parts by weight of the oil extract prepared in step S1, 12 parts by weight of the polysaccharide composition prepared in step S4, 10 parts by weight of the active mixture prepared in step S5, 3 parts by weight of the bifida yeast lysate prepared in step S6 and 1 part by weight of vitamin C were stirred and mixed for 20 minutes to prepare the active extract.

[0060] Preparation Example 3 Preparation of active extract

[0061] Here’s how:

[0062] S1 supercritical flow extraction: 10 parts by weight of Bidens pilosa and 5 parts by weight of Dendrobium candidum were dried, crushed, and subjected to supercritical fluid extraction to obtain an oil extract, and the remaining material was retained;

[0063] The supercritical fluid extraction conditions are: pressure 35 MPa, temperature 37°C, and time 90 min;

[0064] S2. deep eutectic solvent enzymatic hydrolysis: 10 parts by weight of the remaining material in step S1 was added to 60 parts by weight of an aqueous solution containing a deep eutectic solvent, and a composite enzyme was added, wherein the amount of the composite enzyme added was 2 wt % of the total mass of the system, and the enzymatic hydrolysis was carried out at 50° C. for 4 h, and the enzyme was inactivated to obtain an enzymatic hydrolysis product;

[0065] The water content in the aqueous solution containing the deep eutectic solvent is 60 wt %, and the deep eutectic solvent is prepared by mixing choline chloride and glucose in a molar ratio of 1:1;

[0066] The complex enzyme is composed of a mixture of cellulase and pectinase, with a mass ratio of 15:5;

[0067] S3 fermentation: 10 parts by weight of the enzymatic hydrolysate obtained in step S2 was mixed with 30 parts by weight of water, sterilized, inoculated with long bifidobacterium and breve bifidobacterium seed solution, the long bifidobacterium and breve bifidobacterium seed solution inoculation amount were 4v / v% and 3v / v%, respectively, under anaerobic conditions, 39 ° C, 200r / min, fermentation culture 48h, filtered, solid washing, the eluate was collected and concentrated to obtain a bacterial solution, the filtrate was retained;

[0068] S4. Alcohol precipitation: adding ethanol to the filtrate in step S3 to a system ethanol content of 70 wt%, precipitating for 5 h, centrifuging, washing, and drying to obtain a polysaccharide composition, recovering ethanol from the supernatant, and retaining the remaining liquid;

[0069] S5. Macroporous resin purification: The residual liquid in step S4 is decompressed to remove the solvent, and the filtrate is purified by macroporous resin NKA-9, and the solvent is removed by decompression, and the filtrate is diluted with 75wt% ethanol to a concentration of 1.2mg / mL, loaded, and wet packed into a column. The specification of the chromatography column is 15mm×500mm, and the column volume is 50mL. The eluate with a concentration of 75wt% ethanol is collected, the ethanol is removed under reduced pressure, and dried to obtain an active mixture;

[0070] S6. Preparation of bifid yeast lysate: freezing the bacterial solution obtained in step S3 with liquid nitrogen, thawing it at room temperature, centrifuging it, collecting the supernatant, and freeze-drying it to obtain bifid yeast lysate;

[0071] S7. Preparation of active extract: 7 parts by weight of the oil extract prepared in step S1, 15 parts by weight of the polysaccharide composition prepared in step S4, 17 parts by weight of the active mixture prepared in step S5, 5 parts by weight of the bifida yeast lysate prepared in step S6 and 2 parts by weight of vitamin C were stirred and mixed for 20 minutes to prepare the active extract.

[0072] Preparation Example 4 Preparation of active extract

[0073] Here’s how:

[0074] S1 supercritical flow extraction: 8 parts by weight of Bidens pilosa and 4 parts by weight of Dendrobium candidum were dried, crushed, and subjected to supercritical fluid extraction to obtain an oil extract, and the remaining material was retained;

[0075] The supercritical fluid extraction conditions are: pressure 30 MPa, temperature 35°C, time 85 min;

[0076] S2. Deep eutectic solvent enzymatic hydrolysis: 10 parts by weight of the remaining material in step S1 was added to 45 parts by weight of an aqueous solution containing a deep eutectic solvent, and a composite enzyme was added, wherein the amount of the composite enzyme added was 1.5 wt % of the total mass of the system, and the enzymatic hydrolysis was carried out at 45° C. for 3 h, and the enzyme was inactivated to obtain an enzymatic hydrolysis product;

[0077] The water content in the aqueous solution containing the deep eutectic solvent is 50 wt %, and the deep eutectic solvent is prepared by mixing choline chloride and glucose in a molar ratio of 1:1;

[0078] The complex enzyme is composed of a mixture of cellulase and pectinase, with a mass ratio of 12:4;

[0079] S3. Fermentation: 10 parts by weight of the enzymatic hydrolysate obtained in step S2 was mixed with 22 parts by weight of water, sterilized, inoculated with Bifidobacterium longum and Bifidobacterium breve seed solution, the inoculation amount of Bifidobacterium longum and Bifidobacterium breve seed solution was 3v / v% and 2v / v%, respectively, under anaerobic conditions, 37°C, 170r / min, fermented and cultured for 36h, filtered, washed with solids, and the eluate was collected and concentrated to obtain a bacterial solution, and the filtrate was retained;

[0080] S4. Alcohol precipitation: adding ethanol to the filtrate in step S3 to an ethanol content of 65 wt % in the system, precipitating for 4 h, centrifuging, washing, and drying to obtain a polysaccharide composition, recovering ethanol from the supernatant, and retaining the remaining liquid;

[0081] S5. Macroporous resin purification: The residual liquid in step S4 is decompressed to remove the solvent, and the filtrate is purified by macroporous resin NKA-9, and the solvent is removed by decompression, and the filtrate is diluted with 70wt% ethanol to a concentration of 1 mg / mL, loaded, and wet packed into a column. The specification of the chromatography column is 15mm×500mm, and the column volume is 50mL. The eluate with a concentration of 70wt% ethanol is collected, the ethanol is removed under reduced pressure, and dried to obtain an active mixture;

[0082] S6. Preparation of bifid yeast lysate: freezing the bacterial solution obtained in step S3 with liquid nitrogen, thawing it at room temperature, centrifuging it, collecting the supernatant, and freeze-drying it to obtain bifid yeast lysate;

[0083] S7. Preparation of active extract: 6 parts by weight of the oil extract prepared in step S1, 13 parts by weight of the polysaccharide composition prepared in step S4, 14 parts by weight of the active mixture prepared in step S5, 4 parts by weight of the bifida yeast lysate prepared in step S6 and 1.5 parts by weight of vitamin C were stirred and mixed for 20 minutes to prepare the active extract.

[0084] Preparation Example 5

[0085] Compared with Preparation Example 4, the difference is that the composite enzyme is a single cellulase.

[0086] Preparation Example 6

[0087] Compared with Preparation Example 4, the difference is that the complex enzyme is a single pectinase.

[0088] Comparative Preparation Example 1

[0089] Compared with Preparation Example 4, the difference is that Bidens pilosa L. is not added in step S1.

[0090] Comparative Preparation Example 2

[0091] Compared with Preparation Example 4, the difference is that Dendrobium officinale is not added in step S1.

[0092] Comparative Preparation Example 3

[0093] Compared with Preparation Example 4, the difference is that no complex enzyme is added in step S2.

[0094] Comparative Preparation Example 4

[0095] Compared with Preparation Example 4, the difference is that the seed liquid of Bifidobacterium longum is not inoculated in step S3.

[0096] Comparative Preparation Example 5

[0097] Compared with Preparation Example 4, the difference is that the seed liquid of Bifidobacterium breve is not inoculated in step S3.

[0098] Comparative Preparation Example 6

[0099] Compared with Preparation Example 4, the difference is that step S3 is not performed.

[0100] Comparative Preparation Example 7

[0101] Compared with Preparation Example 4, the difference is that no oil extract is added in step S7.

[0102] Comparative Preparation Example 8

[0103] Compared with Preparation Example 4, the difference is that no polysaccharide composition is added in step S7.

[0104] Comparative Preparation Example 9

[0105] Compared with Preparation Example 4, the difference is that no active mixture is added in step S7.

[0106] Comparative Preparation Example 10

[0107] Compared with Preparation Example 4, the difference is that no bifida yeast lysate is added in step S7.

[0108] Example 1

[0109] This embodiment provides a skin tightening and anti-wrinkle essence, and the preparation method comprises the following steps:

[0110] (1) mixing ethoxydiglycol and the permeation enhancing compound prepared in Preparation Example 1 in a mass ratio of 4:3 to obtain a permeation enhancing agent composition;

[0111] (2) mixing palmitoyl tripeptide-5, hexapeptide-11, hexapeptide-9, acetyl hexapeptide-8, and arginine / lysine polypeptide in a mass ratio of 4:1:2:0.5:3 to obtain a polypeptide composition;

[0112] (3) 7 parts by weight of the active extract prepared in Preparation Example 2, 5 parts by weight of the polypeptide composition, 0.3 parts by weight of soluble collagen, 0.1 parts by weight of sodium DNA, 5 parts by weight of hydroxypropyl tetrahydropyrantriol, 0.1 parts by weight of hirudin, 5 parts by weight of butylene glycol, 0.2 parts by weight of carbomer, and 0.02 parts by weight of disodium EDTA were added to 50 parts by weight of deionized water, heated to 70° C., and stirred at 300 r / min for 10 min to prepare phase A.

[0113] (4) adding 0.5 parts by weight of the penetration enhancer composition to 2 parts by weight of capric triglyceride and 0.5 parts by weight of sorbitan olivate, heating to 70° C., and stirring at 300 rpm for 10 min to obtain phase B;

[0114] (5) Add phase B into phase A, stir at 300 r / min for 5 min, emulsify at 7000 r / min for 10 min, keep warm for 10 min, cool down, and discharge to obtain a firming skin anti-wrinkle essence.

[0115] Example 2

[0116] This embodiment provides a skin tightening and anti-wrinkle essence, and the preparation method comprises the following steps:

[0117] (1) mixing ethoxydiglycol and the permeation enhancing compound prepared in Preparation Example 1 in a mass ratio of 7:3 to obtain a permeation enhancing agent composition;

[0118] (2) mixing palmitoyl tripeptide-5, hexapeptide-11, hexapeptide-9, acetyl hexapeptide-8, and arginine / lysine polypeptide in a mass ratio of 6:3:4:1:5 to prepare a polypeptide composition;

[0119] (3) 10 parts by weight of the active extract prepared in Preparation Example 3, 7 parts by weight of the polypeptide composition, 0.5 parts by weight of soluble collagen, 0.5 parts by weight of sodium DNA, 10 parts by weight of hydroxypropyl tetrahydropyrantriol, 0.5 parts by weight of hirudin, 10 parts by weight of trehalose, 0.5 parts by weight of polyethylene glycol 2000, and 0.05 parts by weight of disodium EDTA were added to 70 parts by weight of deionized water, heated to 80° C., and stirred at 500 r / min for 20 min to prepare phase A.

[0120] (4) adding 2 parts by weight of the penetration enhancer composition to 8 parts by weight of polydimethylsiloxane and 2 parts by weight of cetearyl olive oil ester, heating to 80° C., and stirring at 500 r / min for 20 min to prepare phase B;

[0121] (5) Add phase B into phase A, stir at 500 r / min for 15 min, emulsify at 10,000 r / min for 20 min, keep warm for 20 min, cool down, and discharge to obtain a firming skin anti-wrinkle essence.

[0122] Example 3

[0123] This embodiment provides a skin tightening and anti-wrinkle essence, and the preparation method comprises the following steps:

[0124] (1) mixing ethoxydiglycol and the permeation enhancing compound prepared in Preparation Example 1 in a mass ratio of 5:3 to obtain a permeation enhancing agent composition;

[0125] (2) mixing palmitoyl tripeptide-5, hexapeptide-11, hexapeptide-9, acetyl hexapeptide-8, and arginine / lysine polypeptide in a mass ratio of 5:2:3:0.7:4 to obtain a polypeptide composition;

[0126] (3) 8 parts by weight of the active extract prepared in Preparation Example 4, 6 parts by weight of the polypeptide composition, 0.4 parts by weight of soluble collagen, 0.3 parts by weight of sodium DNA, 7 parts by weight of hydroxypropyl tetrahydropyrantriol, 0.3 parts by weight of hirudin, 7 parts by weight of sodium hyaluronate, 0.35 parts by weight of xanthan gum, and 0.035 parts by weight of disodium EDTA were added to 60 parts by weight of deionized water, heated to 75° C., and stirred at 400 r / min for 15 min to prepare phase A.

[0127] (4) adding 1 part by weight of the penetration enhancer composition to 5 parts by weight of glyceryl tri(ethylhexanoate) and 1 part by weight of glyceryl stearate, heating to 75° C., and stirring at 400 r / min for 15 min to obtain phase B;

[0128] (5) Add phase B into phase A, stir at 400 r / min for 10 min, emulsify at 8000 r / min for 15 min, keep warm for 15 min, cool down, and discharge to obtain a firming skin anti-wrinkle essence.

[0129] Example 4

[0130] Compared with Example 3, the difference is that the penetration enhancer composition is replaced by a single ethoxydiglycol.

[0131] Example 5

[0132] Compared with Example 3, the difference is that the permeation enhancer composition is replaced by a single permeation enhancing compound prepared in Preparation Example 1.

[0133] Example 6

[0134] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Preparation Example 5.

[0135] Example 7

[0136] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Preparation Example 6.

[0137] Comparative Example 1

[0138] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 1.

[0139] Comparative Example 2

[0140] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 2.

[0141] Comparative Example 3

[0142] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 3.

[0143] Comparative Example 4

[0144] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 4.

[0145] Comparative Example 5

[0146] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 5.

[0147] Comparative Example 6

[0148] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 6.

[0149] Comparative Example 7

[0150] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 7.

[0151] Comparative Example 8

[0152] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 8.

[0153] Comparative Example 9

[0154] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 9.

[0155] Comparative Example 10

[0156] Compared with Example 3, the difference is that the active extract prepared in Preparation Example 4 is replaced by the active extract prepared in Comparative Preparation Example 10.

[0157] Comparative Example 11

[0158] Compared with Example 3, the difference is that no penetration enhancer composition is added.

[0159] Comparative Example 12

[0160] Compared with Example 3, the difference is that no polypeptide composition is added.

[0161] Comparative Example 13

[0162] Compared with Example 3, the difference is that no active extract is added.

[0163] Test Example 1 Safety Evaluation

[0164] The skin tightening and anti-wrinkle essences prepared in Examples 1-7 and Comparative Examples 1-13 of the present invention were evaluated for safety. The results are as follows:

[0165] Hygienic chemistry and microbiological indicators: lead content is less than 10mg / kg, mercury is less than 0.05mg / kg, arsenic is less than 1mg / kg; total bacteria is less than 10cfu / mL, and fecal coliform group, Staphylococcus aureus, and Pseudomonas aeruginosa are not detected. The above indicators are in compliance with the provisions of the national "Cosmetic Hygiene Standard" GB7916-87.

[0166] Acute oral toxicity test in mice, acute oral toxicity LD for female and male mice 50 The values ​​are all greater than 5000 mg / kg body weight, which is considered to be practically non-toxic according to GB7919-87 Chemical Acute Toxicity Classification Standard.

[0167] In the skin multiple irritation test, the skin irritation score is greater than 0.4 and less than 0.5, and the pathological histology score is < 4. According to the national "Cosmetic Hygiene Standard" GB7916-87 Chemical Skin Irritation Intensity Evaluation Standard, it is a mild irritant.

[0168] Test Example 2: DPPH· Removal Rate

[0169] The skin tightening and anti-wrinkle essences prepared in Examples 1-7 and Comparative Examples 1-13 were diluted with deionized water to 1 mg / mL as sample solutions. Vitamin C was used as a control group.

[0170] Take 2.00×10 -4 3 mL of 1 mol / L DPPH·anhydrous ethanol solution and 3 mL of sample solution were added to the test tube, shaken and placed away from light for 30 min. The absorbance of the mixed solution at 517 nm was measured by A i , and simultaneously measure the absorbance A0 of 3 mL of pure water mixed with 3 mL of DPPH·anhydrous ethanol solution and the absorbance A of 3 mL of sample solution mixed with 3 mL of anhydrous ethanol. j The scavenging rate (%) of DPPH· was calculated according to the formula. The results are shown in Table 1.

[0171] DPPH· removal rate (%) = [1-(A i -A j ) / A0]×100%

[0172] Table 1

[0173]

[0174]

[0175] It can be seen from the above table that the skin tightening and anti-wrinkle essences prepared in Examples 1-3 of the present invention have good antioxidant activity.

[0176] Test Example 3

[0177] The face was selected as the test site, and eligible subjects were selected and divided into 20 groups, namely, Examples 1-7 and Comparative Examples 1-13, with 10 subjects in each group. The subjects used the skin tightening and anti-wrinkle essences prepared in Examples 1-7 and Comparative Examples 1-13 as test products according to the use requirements, collected skin data at the corresponding test sites of the subjects, and analyzed the changes in skin data before and after the use of the samples in the test areas.

[0178] Improvement rate (%) = |(last data - initial data)| / initial data × 100%

[0179] The improvement rate here is an absolute value, which ensures that the improvement rate is always positive. In actual testing, after using the essence, the moisture content of the skin stratum corneum increased, the skin elasticity R2 value increased, and the skin firmness F4 value decreased.

[0180] The specific testing process is as follows:

[0181] Test conditions: On the test day, the subjects cleaned their faces with the designated cleansing samples and sat quietly in a laboratory with a temperature of (25±1)°C and a humidity of 45%±5% RH for 30 minutes.

[0182] Test method: Corneometer tests the moisture content of the stratum corneum, and Cutometer tests the elasticity R2 value and firmness F4 value. The results are shown in Table 2.

[0183] (1) Initial skin data of test subjects: Before the experiment begins, the initial skin data of test subjects is collected.

[0184] (2) After the initial data collection is completed, samples are distributed to the subjects to be applied all over the face once a day for 1 week. After 1 week, the final skin data of the subjects are tested under the same test conditions.

[0185] Table 2

[0186] Group Improvement rate of moisture content in skin stratum corneum (%) Skin elasticity R2 value improvement rate (%) Skin firmness F4 value improvement rate (%) Example 1 52.2 23.1 33.2 Example 2 51.9 23.8 32.9 Example 3 53.1 24.2 33.7 Example 4 44.1 19.7 28.9 Example 5 46.9 21.0 30.4 Example 6 50.2 21.4 31.2 Example 7 49.8 21.9 31.5 Comparative Example 1 45.2 16.7 27.5 Comparative Example 2 46.9 17.2 26.4 Comparative Example 3 47.9 20.2 30.6 Comparative Example 4 48.6 20.6 27.1 Comparative Example 5 48.2 19.7 28.3 Comparative Example 6 46.7 16.8 25.9 Comparative Example 7 49.1 16.1 24.4 Comparative Example 8 44.9 20.2 28.7 Comparative Example 9 46.1 18.4 28.4 Comparative Example 10 47.6 17.5 27.6 Comparative Example 11 43.0 18.2 27.1 Comparative Example 12 48.8 15.7 25.1 Comparative Example 13 42.5 14.9 22.7

[0187] It can be seen from the above table that the skin tightening and anti-wrinkle essences prepared in Examples 1-3 of the present invention have good moisturizing, tightening and skin elasticity improving effects.

[0188] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A skin tightening and anti-wrinkle essence, characterized in that: The invention is prepared from the following raw materials by weight: 7-10 parts of active extract, 5-7 parts of polypeptide composition, 0.5-2 parts of penetration enhancer composition, 0.1-0.5 parts of sodium DNA, 0.3-0.5 parts of soluble collagen, 5-10 parts of hydroxypropyl tetrahydropyrantriol, 2-8 parts of emollient, 5-10 parts of humectant, 0.5-2 parts of emulsifier, 0.2-0.5 parts of thickener, 0.1-0.5 parts of hirudin, 0.02-0.05 parts of disodium EDTA and 50-70 parts of deionized water; The penetration enhancer composition comprises ethoxydiglycol and a penetration enhancing compound, and the structural formula of the penetration enhancing compound is shown in Formula I: Wherein, R = C6-C18 alkyl chain; The preparation method of the active extract is as follows: S1. Supercritical fluid extraction: The Bidens pilosa and Dendrobium candidum were dried, crushed, and subjected to supercritical fluid extraction to obtain an oil extract, and the remaining material was retained; S2. deep eutectic solvent enzymatic hydrolysis: adding the remaining material in step S1 to an aqueous solution containing a deep eutectic solvent, adding a composite enzyme for enzymatic hydrolysis, inactivating the enzyme, and obtaining an enzymatic hydrolysis product; the deep eutectic solvent is prepared by mixing choline chloride and glucose, and the composite enzyme is composed of a mixture of cellulase and pectinase; S3 fermentation: the enzymatic product obtained in step S2 was mixed with water, sterilized, inoculated with long Bifidobacterium and breve Bifidobacterium species seed solution, fermented, filtered, washed with solids, the eluate was collected and concentrated to obtain a bacterial solution, and the filtrate was retained; S4. Alcohol precipitation: adding ethanol to the filtrate in step S3, centrifuging, washing, drying, to obtain a polysaccharide composition, recovering ethanol from the supernatant, and retaining the remaining liquid; S5. Purification by macroporous resin: The solvent of the remaining liquid in step S4 is removed under reduced pressure, and the filtrate is purified by macroporous resin, and the solvent is removed under reduced pressure, and the sample is diluted with 65-75wt% ethanol, and the column is wet loaded, and the column volume is 45-55mL, and the eluate with 65-75wt% ethanol concentration is collected, and the ethanol is removed under reduced pressure and dried to obtain an active mixture; S6. Preparation of bifid yeast lysate: freezing the bacterial solution obtained in step S3 with liquid nitrogen, thawing it at room temperature, centrifuging it, collecting the supernatant, and freeze-drying it to obtain bifid yeast lysate; S7. Preparation of active extract: the oil extract prepared in step S1, the polysaccharide composition prepared in step S4, the active mixture prepared in step S5, the bifida yeast lysate prepared in step S6 and vitamin C are uniformly mixed to prepare the active extract.

2. The skin tightening and anti-wrinkle essence according to claim 1, characterized in that: The mass ratio of the ethoxydiglycol to the penetration-promoting compound is 4-7:

3.

3. The skin tightening and anti-wrinkle essence according to claim 1, characterized in that: The synthesis method of the penetration-promoting compound is as follows: dissolving 1,12-dichlorododecane in dichloromethane, dripping a dichloromethane solution of caprolactam and triethylamine, heating to reflux, stirring to react for a first time period, then dripping a dichloromethane solution of menthol, heating to reflux, stirring to react for a second time period, removing the solvent under reduced pressure, separating and purifying by column chromatography, and obtaining the penetration-promoting compound.

4. The skin tightening and anti-wrinkle essence according to claim 3, characterized in that: The molar ratio of the 1,12-dichlorododecane, caprolactam, menthol and triethylamine is 1:0.9-1:0.9-1:4-6, the first time period is 3-5 hours, and the second time period is 4-6 hours.

5. The skin tightening and anti-wrinkle essence according to claim 1, characterized in that: The polypeptide composition is a composition of palmitoyl tripeptide-5, hexapeptide-11, hexapeptide-9, acetyl hexapeptide-8, and arginine / lysine polypeptide, and the mass ratio is 4-6:1-3:2-4:0.5-1:3-5.

6. The skin tightening and anti-wrinkle essence according to claim 1, characterized in that: The mass ratio of Bidens pilosa and Dendrobium officinale described in step S1 is 7-10:3-5, and the conditions of the supercritical fluid extraction are pressure 27-35MPa, temperature 32-37°C, and time 70-90min; the mass ratio of the residual material and the aqueous solution containing a deep eutectic solvent described in step S2 is 10:35-60, the water content in the aqueous solution containing a deep eutectic solvent is 40-60wt%, the molar ratio of choline chloride and glucose is 1:1, the mass ratio of cellulase and pectinase is 10-15:3-5, the addition amount of the composite enzyme is 1-2wt% of the total mass of the system, the temperature of the enzymolysis is 40-50°C, and the time is 2-4h; the mass ratio of the enzymolysis product and water in step S3 is 1:1.5-3, the inoculation amounts of the long bifidobacterium and the short bifidobacterium seed solution are 2-4v / v% and 1-3v / v%, respectively, and the bacterial content of the seed solution is 10 8 -10 9 cfu / mL, and the fermentation culture conditions are anaerobic conditions, 36-39°C, 150-200r / min, and the fermentation culture is 24-48h.

7. The skin tightening and anti-wrinkle essence according to claim 1, characterized in that: In step S4, ethanol is added to the system ethanol content of 60-70wt%, and the precipitation time is 3-5h; in step S5, the macroporous resin is selected from at least one of XDA-2, NKA-9, SP-825, D101, HPD600, X-5, LSA-10, NKI-9 and AB-8, and is diluted with 65-75wt% ethanol to a concentration of 0.8-1.2mg / mL; in step S7, the mass ratio of the oil extract, the polysaccharide composition, the active mixture, the bifida yeast lysate and the vitamin C is 5-7:12-15:10-17:3-5:1-2.

8. The skin tightening and anti-wrinkle essence according to claim 1, characterized in that: The emollient is selected from at least one of tri(ethylhexanoin)glyceryl, isononyl isononanoate, polydimethylsiloxane, caprylic / capric triglyceride, and avocado tree (BUTYROSPERMUM PARKII) fruit butter; the moisturizer is selected from at least one of glycerin, butylene glycol, propylene glycol, hexylene glycol, sodium hyaluronate, betaine, and trehalose; the emulsifier is selected from at least one of glyceryl stearate, PEG-100 stearate, cetearyl olive oil ester, sorbitan olive oil ester, C12-20 alkyl glucoside, and polyglyceryl-2 stearate; the thickener is selected from at least one of carbomer, acrylic acid (ester) / C10-30 alcohol acrylate crosspolymer, sodium polyacryloyldimethyl taurate, and xanthan gum.

9. A method for preparing the skin tightening and anti-wrinkle essence according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) Add the active extract, polypeptide composition, soluble collagen, DNA sodium, hydroxypropyl tetrahydropyrantriol, hirudin, moisturizer, thickener, and disodium EDTA into deionized water, heat to 70-80°C, and stir at 300-500 r / min for 10-20 min to prepare phase A. (2) adding the penetration enhancer composition to the emollient and emulsifier, heating to 70-80° C., and stirring at 300-500 r / min for 10-20 min to obtain phase B; (3) Add phase B into phase A, stir at 300-500 r / min for 5-15 min, emulsify at 7000-10000 r / min for 10-20 min, keep warm for 10-20 min, cool down, discharge, and obtain a firming skin anti-wrinkle essence.

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