Anti-aging composition for preventing light aging and application thereof

Abstract

The invention discloses an anti-aging composition for preventing light aging and application thereof, the anti-aging composition comprises a Rosa rugosa extract, a Caesalpinia spinosa fruit extract, a sunflower bud extract and a Pseudoalteromonas fermentation product extract, and the anti-aging composition can be added into various skin care products to reduce cell damage caused by light radiation such as ultraviolet light, infrared light and blue light; in sunscreen cosmetics, the sunscreen effect can be improved, the use amount of chemical sunscreen agents and physical sunscreen agents is reduced, and the safety and mildness of the sunscreen cosmetics are improved; damage caused by optical radiation is effectively prevented, meanwhile, skin elasticity is improved, skin wrinkles are reduced, and the effect of resisting skin photoaging is achieved. The effectiveness of the composition is proved through a large number of experiments, each component is irreplaceable, the addition range of the composition is also an optimal range, and the effect and cost of the composition are both the optimal range.

Description

Technical Field

[0001] The invention belongs to the field of skin care products, and specifically relates to an anti-aging composition for preventing photoaging and application thereof. Background Art

[0002] Aging can be divided into endogenous aging and exogenous aging. Endogenous aging refers to the aging caused by irresistible factors in the body, such as genetics, endocrine system, and immune function, as we age. Exogenous aging mainly refers to skin aging caused by external stimuli, such as light radiation, air pollution, etc., which lead to oxidative damage to the skin.

[0003] Among the exogenous aging factors, light radiation has a more obvious effect on the skin. With the continuous research and understanding of skin photoaging, the demand for anti-aging skin care products to prevent photoaging is also increasing. The main light radiation that affects skin aging includes ultraviolet light, infrared light, blue light, etc. Ultraviolet radiation is divided into UVA and UVB. UVA affects the dermis of the skin and produces a large amount of reactive oxygen. Reactive oxygen will enhance the expression of nuclear factor-kB, extracellular regulatory proteases, and mitogen-activated protein kinases, causing inflammatory factors (such as interleukin-1, interleukin-6, tumor necrosis factor, etc.) and matrix metalloproteinases (MMPs) to increase, reduce the content of collagen and collagen fibers in the cell matrix, and macroscopically manifest as decreased skin elasticity and deeper wrinkles; the oxidative stress generated by UVA will further cause DNA damage and affect the function of mitochondria, accelerating aging. UVB affects the human epidermal cell layer, directly causing DNA damage, forming cyclobutane pyrimidine dimers, accelerating the generation of reactive oxygen, and aggravating skin aging; long-term UVB irradiation will also cause excessive endoplasmic reticulum stress, damage the skin barrier function, and make the skin more susceptible to light radiation damage. In addition to sunlight, blue light comes from digital screens, light-emitting diodes, fluorescent lamps and other products in our daily lives. Its wavelength is 400nm-500nm, and it affects the epidermis, dermis and subcutaneous tissue. Blue light of different wavelengths and doses has different effects on the skin. For example, blue light with a wavelength of 410nm and 420nm can cause oxidative stress and toxicity in cells, and can reduce the antioxidant capacity of fibroblasts. Excessive exposure to blue light can aggravate oxidative stress, leading to inflammation and damage to the skin barrier. The infrared light that affects skin aging is mainly near-infrared light of 700-1000nm. Like blue light, it affects subcutaneous tissue deeper than UVA and UVB. Near-infrared light can damage the mitochondrial electron transport chain (ETC), resulting in a decrease in the energy (ATP) produced in mitochondria and an increase in reactive oxygen species, an increase in metalloproteinase-1 (MMP-1) that promotes collagen degradation, and a decrease in type I collagen gene expression. In addition, near-infrared light can also activate the thermoreceptor TRPV1, leading to further acceleration of inflammation and skin aging.

[0004] To prevent photoaging, the commonly used methods are: isolating ultraviolet rays through sunscreen cosmetics and physical methods (sun protection umbrellas, sun protection clothing, etc.). These two methods are mainly used for outdoor travel and are not suitable for some daily indoor occasions.

[0005] The 2015 edition of the "Technical Specifications for Safety of Cosmetics" includes 26 types of sunscreens with clear limits on usage, of which 24 are chemical sunscreens and 2 are physical sunscreens, which mainly protect against ultraviolet light and have less protection against blue light and infrared light in the sun. Chemical sunscreens are a class of highly allergenic substances with potential hazards such as skin irritation, sensitization, phototoxicity, carcinogenicity, and genotoxicity. Physical sunscreens are safer than chemical sunscreens, but if not cleaned properly or used for a long time, there is a risk of clogging the skin pores and causing the skin condition to deteriorate. Summary of the invention

[0006] The purpose of the present invention is to overcome at least one deficiency of the prior art and provide an anti-aging composition for preventing photoaging and its application.

[0007] The technical solution adopted by the present invention is:

[0008] In a first aspect, the present invention provides an anti-aging composition for preventing photoaging, the composition comprising the following components: a Cistus floribundus extract, a Veratrum spinulosa fruit extract, a sunflower sprout extract, and a Pseudoalteromonas fermentation product extract.

[0009] In some examples, the mass ratio of the components of the composition is: 0.1-0.5 parts of Cistus floribundus extract, 0.5-5 parts of Veratrum spinulosa fruit extract, 0.5-5 parts of sunflower sprout extract, and 1-9 parts of Pseudoalteromonas fermentation product extract.

[0010] In some examples, the mass ratio of the components of the composition is: 0.1 parts of Cistus floribundus extract, 0.5 parts of Veratrum spinulosa fruit extract, 0.5 parts of sunflower sprout extract, and 1 part of Pseudoalteromonas fermentation product extract.

[0011] In some examples, the mass ratio of the components of the composition is: 0.3 parts of Cistus floribundus extract, 2.75 parts of Veratrum spinulosa fruit extract, 2.75 parts of sunflower sprout extract, and 5 parts of Pseudoalteromonas fermentation product extract.

[0012] In some examples, the mass ratio of the components of the composition is: 0.5 parts of Cistus floribundus extract, 5 parts of Veratrum spinulosa fruit extract, 5 parts of sunflower sprout extract, and 9 parts of Pseudoalteromonas fermentation product extract.

[0013] In a second aspect, the composition provided in the first aspect of the present invention is used in a skin care product, wherein the skin care product comprises auxiliary materials and functional ingredients, and the functional ingredients are the anti-aging composition for preventing photoaging.

[0014] In some examples, the anti-aging composition for preventing photoaging is added in an amount of 2 to 20% by weight in skin care products.

[0015] In some examples, the skin care product is a cream, lotion, water solution, essence, gel, or mask.

[0016] In some examples, the excipients include solvents, humectants, emulsifiers, chelating agents, and preservatives.

[0017] The beneficial effects of the present invention are:

[0018] The present invention is an anti-aging composition for preventing photoaging and its application, which comprises a Cistus floribundus extract, a spinulosa fruit extract, a sunflower bud extract, and a Pseudoalteromonas fermentation product extract, which can be added to various skin care products to reduce cell damage caused by ultraviolet light, infrared light, blue light and other light radiation; in sunscreen cosmetics, it can improve the sunscreen effect, reduce the use of chemical sunscreens and physical sunscreens, and improve the safety and mildness of sunscreen cosmetics; effectively prevent damage caused by light radiation, while improving skin elasticity, reducing skin wrinkles, and achieving the effect of resisting skin photoaging. The present invention proves the effectiveness of the composition of the present invention through a large number of experiments, and each component is irreplaceable, and the addition range of the composition of the present invention is also an optimal range, which is an optimal range in terms of effect and cost. In summary, the composition of the present invention is an economical and effective anti-aging composition for preventing photoaging. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a comparison chart of the changes in skin wrinkles of volunteers before using Example 2 and after using it for 28 days.

[0020] Figure 2 This is a comparison chart of the changes in skin wrinkles of volunteers before using Comparative Example 2 and after using it for 28 days.

[0021] Figure 3 This is a comparison chart of the changes in skin wrinkles of volunteers before using Comparative Example 6 and after using it for 28 days. DETAILED DESCRIPTION

[0022] The following disclosure provides many different embodiments or examples for implementing different solutions of the present invention.

[0023] The efficacy of the ingredients in the composition is described as follows:

[0024] Cistus polyphylla extract: Cistus polyphylla extract can reduce the production of metalloproteinase-1 (MMP-1) caused by blue light, thereby reducing the degradation of collagen; Cistus polyphylla extract can also reduce the generation of free radicals caused by UVA and the inflammation and DNA damage caused by UVB, reducing the damage caused by ultraviolet radiation to the skin; in addition, it can also promote the recovery of the production of extracellular matrix protein 1 (ECM1), thereby maintaining the balance in the skin and making the skin recover better after being stimulated. Human tests have shown that for women (36-56 years old) who often sunbathe and have 2-4 levels of crow's feet, 0.02% Cistus polyphylla extract can reduce 6% of skin roughness and 9% of wrinkles; for women (37-55 years old) who often sunbathe and have 1-3 levels of crow's feet, 0.02% Cistus polyphylla extract can reduce 6% of skin roughness and 10% of fine lines.

[0025] Verbena spinulosa fruit extract: Verbena spinulosa fruit extract is rich in hydrolyzable tannins mainly composed of gallic acid. This hydrolyzable tannin has excellent antioxidant properties and can block the production of reactive oxygen species (ROS) in mitochondria caused by light radiation, thereby reducing the synthesis of metalloproteinase 1 (MMP-1) in cells that leads to collagen degradation and slowing down skin aging caused by light radiation.

[0026] Sunflower sprout extract: Sunflower sprout extract can protect the normal function of mitochondria and significantly help maintain mitochondrial energy production under the influence of external stress. Gene array analysis shows that the extract can also protect collagen in fibroblasts against H2O2-induced oxidative stress. Experiments have shown that sunflower sprout extract can reduce the decline in mitochondrial DNA content after infrared light exposure. According to human experiments, adding thorny fruit extract and sunflower sprout extract to SPF30 sunscreen can enhance skin protection and improve skin quality (compared to before use).

[0027] Pseudoalteromonas fermentation product extract: This Pseudoalteromonas fermentation product extract has the ability to form a film, increase the extracellular matrix components (heparan sulfate, hyaluronic acid) and desmoglein-3 (Dsg-3), and together with the polymorphic rock rose extract, it strengthens the skin barrier strength and repairs the skin barrier damage caused by light radiation; it performs spectral anti-wrinkle by increasing the type I collagen, type III collagen, elastin, type IV collagen, hyaluronic acid, and heparan sulfate in the cell matrix. In addition, the Pseudoalteromonas fermentation product extract can also reduce the increase of metalloproteinase-1 caused by blue light irradiation, thereby reducing the collagen degradation caused by blue light.

[0028] The active components of each component of Examples 1 to 3 of the present invention and Comparative Examples 1 to 5 are shown in Table 1, and the matrix excipients are as follows: Phase A: 0.5% cetearyl glucoside, 2% 1618 alcohol, 2% polydimethylsiloxane, 2% squalane, 1% shea butter, 0.5% vitamin E, 6% isononyl isononanoate; Phase B: 0.5% acrylic acid compound, 0.03% disodium EDTA, 3% pentanediol, 0.5% hexylene glycol, 6% glycerol; Phase C: 0.5% arginine. The balance is water.

[0029] Table 1

[0030]

[0031] illustrate:

[0032] 1. Comparative Example 1 is an essence cream prepared by replacing the composition of the present invention in the essence cream of Example 2 of the present invention with retinol, a star ingredient in anti-aging products currently on the market.

[0033] 2. Comparative Example 2 is an essence cream prepared by replacing the composition of the present invention in the essence cream of Example 2 of the present invention with acetyl hexapeptide-8, which is widely used in anti-aging products on the market and has a good anti-aging effect.

[0034] 3. Comparative Example 3 is an essence cream obtained by removing the Cistus polyphylla extract from the essence cream of Example 2 of the present invention.

[0035] 4. Comparative Example 4 is an essence cream obtained by removing the eucalyptus thorn fruit extract and the sunflower sprout extract from the essence cream of Example 2 of the present invention.

[0036] 5. Comparative Example 5 is an essence cream obtained by removing the Pseudoalteromonas fermentation product in the essence cream of Example 2 of the present invention.

[0037] 6. Comparative Example 6 is an international brand anti-aging essence cream with good sales and reputation on the market.

[0038] Efficacy verification

[0039] (I) Efficacy test

[0040] 1. Test equipment:

[0041] 1. The present invention uses the Delfin ElastiMeter (skin elasticity meter) from Finland. The ElastiMeter uses a pressure head to lightly press on the skin. When external force is applied to the skin, the skin resists the change in its shape. The response of the skin under short-term load indicates its immediate elastic properties, thereby obtaining the elasticity value of the skin.

[0042] 2. Take photos using Delfin Antera 3D from Finland. Antera3D uses innovative optical methods and complex mathematical algorithms to obtain three-dimensional images. This allows data to be extracted from images related to the three-dimensional shape of the skin, which will be able to quantify the effects of treatment and monitor changes over time. For example, measure the texture, smoothness, and depth and width of fine lines and wrinkles of the skin. With its multispectral analysis, Antera software can also measure pigmentation and redness, which will provide meaningful information about sun damage, veins, etc.

[0043] 3. Use the Finnish Delfin VapoMeter SWL5201 transepidermal water loss meter. The VapoMeter has a cylindrical chamber with relative humidity and temperature sensors inside. Shortly after the instrument is in contact with the skin, the relative humidity (RH%) in the measurement chamber increases linearly. The TEWL value is calculated based on the increase in RH%. In the measurement interval, the measurement chamber is passively ventilated. The VapoMeter uses the closed chamber measurement principle, so the measurement is not affected by the air flow in the room. The short measurement time ensures that the blocking of normal evaporation is minimized.

[0044] 2. Experimental Principle:

[0045] The characteristic changes of skin caused by photoaging are mainly manifested as dry skin, sagging, wrinkles, increased or lost pigments, and capillary dilation, etc. Based on this characteristic, human tests were conducted to test the transepidermal water loss (TEWL value), skin elasticity (ISE value), skin wrinkle length (WRC value), average wrinkle width (WRK), and average wrinkle depth (WRS) of volunteers before and after using the product, and the anti-photoaging ability of the product was evaluated based on the test results.

[0046] Transepidermal water loss (TEWL value) measures the change in water vapor pressure near the skin surface. The TEWL value indicates the loss of water from the stratum corneum and is an important criterion for evaluating the barrier function of the stratum corneum. The lower the TEWL value of the skin, the better the barrier function of the skin, and vice versa.

[0047] The elasticity value (ISE value) of the skin is measured using the Delfin ElastiMeter (skin elasticity meter) from Finland. The ElastiMeter uses a pressure head to gently press on the skin. When external force is applied to the skin, the skin resists changes in its shape. The response of the skin under short-term load indicates its immediate elastic properties, thereby obtaining the elasticity value (ISE value) of the skin. The higher the ISE value, the better the skin elasticity.

[0048] Skin wrinkle length (WRC value), wrinkle width average (WRK), wrinkle depth average (WRS), using Finland Delfin Antera 3D to take pictures and extract data from images related to the three-dimensional shape of the skin, which will be able to quantify the treatment effect and monitor changes over time. The smaller the skin wrinkle length (WRC value), wrinkle width average (WRK), and wrinkle depth average (WRS), the lighter the wrinkle growth.

[0049] 3. Test population:

[0050] There are 8 groups in total, with 20 people in each group, including volunteers aged 30-65 years old who meet the experimental requirements.

[0051] 4. Test conditions:

[0052] At room temperature of 21±1°C and relative humidity of 50±10%, real-time dynamic monitoring was performed and the subjects sat quietly for 30 minutes before relevant data were tested.

[0053] 5. Experimental process:

[0054] The experiment was divided into 8 groups. The first group used Example 1 of the present invention on the whole face, the second group used Example 3 of the present invention on the whole face, the third group used Comparative Example 6 on the whole face, the 4-8 groups used Example 2 of the present invention on the left face, and each group applied Comparative Examples 1-5 on the right face; rubbed it on the face twice a day, morning and evening, for 4 weeks, and recorded the skin elasticity ISE value, transepidermal water loss rate TEWL value, average skin wrinkle length WRC (mm), average wrinkle width WRK (mm), average wrinkle depth WRS (mm), and then took photos of each volunteer's left and right forehead, eye corners, and cheeks with Antera 3D, and archived them.

[0055] 6. Experimental results:

[0056] 1. Transepidermal water loss (TEWL) test

[0057] In this test, the TEWL measured by the subjects in week 0 is set as TEWL0, and the TEWL in week 4 is set as TEWL4. The TEWL change value is △TEWL, △TEWL = TEWL4-TEWL0, △TEWL change rate = (TEWL4-TEWL0) / TEWL0*100%, and the △TEWL change rate is a negative number. The lower the value, the less water loss of the skin after four weeks of use, that is, the better the skin barrier function is repaired, the better the skin retains moisture, and the healthier the skin. Table 2 shows the average △TEWL change rate value of 20 people in each group compared with the initial value after 4 weeks of use, as follows:

[0058] Table 2 △TEWL change rate (%)

[0059]

[0060]

[0061] 2. Skin elasticity (ISE) test

[0062] In this test, the ISE measured by the subjects in week 0 is set as ISE0, and the ISE in week 4 is set as ISE4. The ISE change value is △ISE, △ISE=ISE4-ISE0, △ISE change rate=(ISE4-ISE0) / ISE0*100%, and the △ISE change rate is a positive number. The higher the value, the more the skin elasticity value increases after using the product for four weeks, the higher the skin elasticity, and the tighter, plumper and more elastic the skin. Table 3 shows the average △ISE change rate value of 20 people in each group compared with the initial value after 4 weeks of use, as follows:

[0063] Table 3 △ISE change rate (%)

[0064] Group △ISE change rate (%) Example 1 11.43 Example 2 20.00 Example 3 28.21 Comparative Example 1 10.65 Comparative Example 2 7.79 Comparative Example 3 4.30 Comparative Example 4 5.86 Comparative Example 5 4.71 Comparative Example 6 7.67

[0065] 3. Skin wrinkle length (WRC value), wrinkle width average (WRK), wrinkle depth average (WRS) test

[0066] In this test, the average values ​​of wrinkle length, width and depth measured by the subjects in week 0 are set as WRC0, WRK0 and WRS0, and the average values ​​of wrinkle length, width and depth measured in week 4 are set as WRC4, WRK4 and WRS4. The wrinkle change values ​​are △WRC, △WRK and △WRS, where △WRC = WRC4-WRC0, △WRK = WRK4-WRK0 and △WRS = WRS4-WRS0; △WRC change rate = (WRC4-WRC0) / WRC0*100%, △WRK change rate = (WRK4-WRK0) / WRK0*100%, △WRS change rate = (WRS4-WRS0) / WRS0*100%; the △WRC, △WRK and △WRS change rates are negative numbers, and the lower the values ​​are, the shorter the length of the skin wrinkles are, the narrower the width is, and the shallower the depth is, the better the product's ability to delay the formation of wrinkles on the skin is, and the smoother and younger the skin is. Table 4 shows the average change rate of wrinkle length, width and depth of 20 people in each group after 4 weeks of use compared with the initial value, as follows:

[0067] Table 4 △WRC, △WRK, △WRS change rate (%)

[0068] Group △WRC change rate (%) △WRK change rate (%) △WRS change rate (%) Example 1 -10.87 -12.72 -15.56 Example 2 -32.96 -19.43 -24.03 Example 3 -35.81 -24.69 -29.6 Comparative Example 1 -11.34 -9.59 -13.17 Comparative Example 2 -10.08 -7.32 -7.42 Comparative Example 3 -13.45 -9.02 -8.64 Comparative Example 4 -11.59 -13.24 -12.12 Comparative Example 5 -16.24 -15.95 -10.87 Comparative Example 6 -3.01 -6.23 -6.16

[0069] Experimental results:

[0070] 1. From the experimental results in Table 2, we can see that:

[0071] 1. △TEWL change rate value, Example 1> Example 2> Example 3, which shows that as the addition ratio of the composition of the present invention increases, the △TEWL change rate value will become smaller accordingly, that is, the less the skin moisture loss is, the more complete the stratum corneum barrier function of the skin is, the better the skin's ability to resist external environmental damage is, the less likely the skin is to lose moisture, and the skin will be healthier, more hydrated, and fuller.

[0072] 2. The △TEWL change rate value of comparative example 1 is 15.44%, indicating that after using the essence cream of comparative example 1, the skin loses more water, which means that the barrier function of the stratum corneum of the skin gradually weakens, the skin becomes more fragile and dry, and the skin loses water more easily.

[0073] 3. The △TEWL change rate value of Comparative Example 2 is -3.85%, that is, the water loss rate of the essence cream of Comparative Example 2 does not increase significantly, indicating that the use of the essence cream of Comparative Example 2 does not have a better effect on the recovery of the skin barrier function.

[0074] 4. Comparative Examples 3-5 are essence creams made by removing any of the compositions of the present invention in Example 2. By comparing with Example 2, their △TEWL change rates are all greater than that of Example 2. Therefore, it can be explained that arbitrarily removing any component in the composition of the present invention will increase the corresponding △TEWL value, that is, the skin moisture is more easily lost, and the recovery of the skin's stratum corneum barrier function is not as good as that of Example 2.

[0075] 5. The △TEWL change rate values ​​of Examples 1-3 are all smaller than those of Comparative Example 6, which indicates that the ability of the composition of the present invention to reduce skin moisture loss within the range of added amount is better than that of the anti-aging essence cream of international brands on the market, and the ability to maintain skin moisture and repair the skin stratum corneum barrier is also better than that of the anti-aging essence cream of international brands on the market.

[0076] 2. From the experimental results Table 3, we can see that:

[0077] 1. △ISE change rate value, Example 1 < Example 2 < Example 3, which shows that as the addition ratio of the composition of the present invention increases, the change rate of its elasticity value is also greatly improved. Therefore, it is shown that within the addition range of the composition of the present invention, the higher the addition amount of each component, the more the skin elasticity increases, and the firmer and fuller the skin is.

[0078] 2. The ΔISE change rate value of Comparative Example 1 is less than that of Example 2, which indicates that even if the composition of the present invention is replaced with retinol, a star anti-aging ingredient on the market, and the amount of retinol added is the highest, the effect of increasing skin elasticity is still not as good as that of the composition of the present invention.

[0079] 3. The ΔISE change rate value of Comparative Example 2 is less than the ΔISE change rate value of Example 2, which indicates that even if the composition of the present invention is replaced with a peptide that is widely used on the market and the added amount is similar to that in Example 2, the effect of increasing skin elasticity is still far inferior to that of the composition of the present invention.

[0080] 4. Comparative Examples 3-5 are essence creams made by removing any of the compositions of the present invention in Example 2. By comparing with Example 2, their ΔISE change rates are all smaller than those of Example 2. Therefore, it can be explained that arbitrarily removing any component in the composition of the present invention will reduce the corresponding ΔISE change rate value, and the increase in skin elasticity is not as good as that of Example 2 of the present invention.

[0081] 5. The ΔISE change rate values ​​of Examples 1-3 are all greater than those of Comparative Example 6, which indicates that the ability of the composition of the present invention to increase skin elasticity within the range of added amount is better than that of the anti-aging essence cream of international brands on the market, and the cosmetics made using the composition of the present invention have a more excellent effect of increasing skin elasticity and firming the skin.

[0082] 3. From the experimental results in Table 4, we can see that:

[0083] 1. The change rate values ​​of △WRC, △WRK and △WRS are as follows: Example 1> Example 2> Example 3. It can be shown that as the addition ratio of the composition of the present invention increases, the change rate values ​​of △WRC, △WRK and △WRS will decrease accordingly, that is, the shorter the length of the wrinkles on the skin, the narrower the width and the shallower the depth, which means that the overall wrinkles on the skin become less obvious and the anti-aging effect of the product becomes more excellent.

[0084] 2. The change rate values ​​of △WRC, △WRK and △WRS of comparative example 1 are greater than those of example 2, indicating that after using the essence cream of comparative example 1, the overall ability of reducing skin wrinkles is not as good as that of example 2 of the present invention, that is, the composition of the present invention is worse than 1% retinol in reducing the overall level of wrinkles.

[0085] 3. The change rate values ​​of △WRC, △WRK and △WRS of Comparative Example 2 are greater than those of Example 2, indicating that after using the essence cream of Comparative Example 2, the overall ability of reducing skin wrinkles is not as good as that of Example 2 of the present invention, that is, the composition of the present invention is worse than acetyl hexapeptide-8 with the same added amount in reducing the overall level of wrinkles.

[0086] 4. Comparative Examples 3-5 are essence creams made by removing any one of the compositions of the present invention in Example 2. By comparing with Example 2, their △WRC, △WRK, and △WRS change rate values ​​are all greater than those of Example 2. Therefore, it can be explained that any removal of any component in the composition of the present invention will increase the corresponding △WRC, △WRK, and △WRS change rate values, that is, the overall level of skin wrinkles will be more. Therefore, any removal of any one of the compositions of the present invention will reduce the anti-wrinkle effect of the product, that is, any component in the composition of the present invention has a positive effect on the anti-aging effect of the final product.

[0087] 5. The change rate values ​​of △WRC, △WRK and △WRS of Examples 1-3 are all smaller than those of Comparative Example 7, which indicates that the ability of the composition of the present invention to reduce the overall level of skin wrinkles within the addition amount range is better than that of the anti-aging essence cream of international brands on the market, that is, the anti-wrinkle and anti-aging effects are better than those of the anti-aging essence cream of international brands on the market.

[0088] 6. Test picture comparison

[0089] A part of the facial wrinkle images of volunteers using the embodiment of the present invention were selected for comparison before and after use. Figure 1-3 It can be seen intuitively from the figure that after using the essence cream prepared by the embodiment of the present invention on the face, the overall level of facial wrinkles is reduced, indicating that the anti-wrinkle effect of the composition of the present invention is very excellent.

[0090] (II) Safety test (human skin patch test)

[0091] 1. Number of testers: 40 people in total, 20 males and 20 females, aged between 18 and 60 years old.

[0092] 2. Health status: The subject has healthy skin, no history of skin allergies, and meets the voluntary inclusion criteria.

[0093] 3. Test method: Select a qualified patch tester and use a closed patch test method. Place about 0.020-02025g of the test product in the patch tester and cover it on the back of the subject with a special adhesive tape. Each subject has 11 application points. Remove the test article after 24 hours and observe the skin reaction 0.5, 24, and 48 hours after removal. Score according to the skin reaction grading standard in the "Cosmetic Hygiene Standards", calculate the average value, and record the results in Table 6.

[0094] 4. See Table 5 for evaluation criteria.

[0095] Table 5 Human skin patch test standard table

[0096]

[0097]

[0098] It can be seen from Table 6 that the various components of the composition of the present invention are very safe, very friendly to the skin, mild and non-irritating, while Comparative Example 1 has a relatively strong irritation and Comparative Example 6 has a weak irritation.

[0099] Table 6 Skin patch test scoring results

[0100] Rating 0.5(h) 24(h) 48(h) Example 1 0 0 0 Example 2 0 0 0 Example 3 0 0 0 Comparative Example 1 3 1 1 Comparative Example 2 0 0 0 Comparative Example 3 0 0 0 Comparative Example 4 0 0 0 Comparative Example 5 0 0 0 Comparative Example 6 1 0 0

[0101] The above is a further detailed description of the present invention, which should not be regarded as a limitation on the specific implementation of the present invention. For ordinary technicians in the technical field to which the present invention belongs, simple deduction or replacement without departing from the concept of the present invention is within the protection scope of the present invention.

Claims

1. An anti-aging composition for preventing photoaging, characterized in that: The composition comprises the following components: Cistus floribundus extract, Caesalpinia spinulosa fruit extract, sunflower sprout extract, and Pseudoalteromonas fermentation product extract.

2. The composition according to claim 1, characterized in that The mass ratio of the components of the composition is: 0.1-0.5 parts of Cistus floribundus extract, 0.5-5 parts of Veratrum spinulosa fruit extract, 0.5-5 parts of sunflower sprout extract, and 1-9 parts of Pseudoalteromonas fermentation product extract.

3. The composition according to claim 2, characterized in that The mass ratio of the components of the composition is: 0.1 part of Cistus floribundus extract, 0.5 part of Caesalpinia spinulosa fruit extract, 0.5 part of sunflower sprout extract, and 1 part of Pseudoalteromonas fermentation product extract.

4. The composition according to claim 2, characterized in that The mass ratio of the components of the composition is: 0.3 parts of Cistus floribundus extract, 2.75 parts of Caesalpinia spinulosa fruit extract, 2.75 parts of sunflower sprout extract, and 5 parts of Pseudoalteromonas fermentation product extract.

5. The composition according to claim 2, characterized in that The mass ratio of the components of the composition is: 0.5 parts of Cistus floribundus extract, 5 parts of Caesalpinia spinulosa fruit extract, 5 parts of sunflower sprout extract, and 9 parts of Pseudoalteromonas fermentation product extract.

6. Use of the composition according to any one of claims 1 to 5 in skin care products, characterized in that: The skin care product comprises auxiliary materials and functional ingredients, and the functional ingredients are the anti-aging composition for preventing photoaging.

7. The use according to claim 6, characterized in that: The anti-aging composition for preventing photoaging is added in a skin care product in an amount of 2 to 20% by weight.

8. The use according to claim 6, characterized in that: The skin care products are creams, lotions, water solutions, essences, gels, and facial masks.

9. The use according to claim 6, characterized in that: The auxiliary materials include solvents, moisturizers, emulsifiers, chelating agents and preservatives.

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