Anti-hair loss composition containing cacumen biotae as well as preparation method and application of anti-hair loss composition

Abstract

The invention provides an anti-hair loss composition containing cacumen biotae as well as a preparation method and application of the anti-hair loss composition, and belongs to the technical field of biological medicines. The anti-hair loss composition containing cacumen biotae comprises a cacumen biotae extract, a ginseng root extract, an eclipta prostrata extract, a polygonum multiflorum extract, a tea seed extract, a gleditsia sinensis extract, a mulberry bark extract, a cortex dictamni extract, a cnidium monnieri seed extract and a mint extract. All the components have a synergistic effect, and the shampoo has the effects of improving the greasy environment of scalp, preventing grease from blocking hair follicles, reducing the damage of inflammation to the hair follicles, inhibiting the activity of 5 alpha-reductase, improving the color and toughness of hair and the like, relieves alopecia seborrhoeica and achieves an anti-alopecia effect.

Description

Technical Field

[0001] The invention relates to the technical field of biopharmaceuticals, and in particular to an anti-hair loss composition containing Platycladus orientalis leaves, and a preparation method and application thereof. Background Art

[0002] With the accelerated pace of life in modern society, the increase in work pressure and the change in living environment, hair loss has become more and more common, seriously affecting people's quality of life and mental health. According to relevant statistics, the number of people with hair loss has been increasing year by year worldwide, reaching hundreds of millions, and the number is getting younger. Hair loss not only damages personal image, but may also cause psychological problems such as inferiority and anxiety, causing many negative impacts on patients' social, work and daily life.

[0003] At present, some chemical anti-hair loss products on the market have certain effects, but there are many side effects. For example, the common finasteride is only suitable for male androgenic alopecia, and is prohibited for women, and may cause adverse reactions such as loss of libido and erectile dysfunction; although minoxidil can be used by both men and women, some users will experience allergic symptoms such as scalp irritation, itching, and redness. Long-term use may also cause drug dependence. Once the drug is stopped, hair loss may recur and worsen. Physical therapy methods, such as laser therapy, stimulate hair follicles through low-energy lasers to promote hair growth, but they require long-term use, and the equipment is expensive and the treatment cost is high, which is difficult for ordinary families to afford. In addition, frequent use of laser equipment may cause potential damage to scalp tissue and affect the normal physiological function of the skin. Hair transplant surgery is a more direct way to solve the problem of hair loss, but the operation is an invasive operation with risks of infection, bleeding, and scar formation. Moreover, hair transplant surgery is expensive and requires high hair follicle resources from the patient himself, so it is not suitable for all hair loss patients. At the same time, the survival rate of hair follicles after hair transplantation is not 100%, and multiple surgeries may be required, increasing the patient's pain and financial burden.

[0004] The application of natural plant extracts in anti-hair loss products has attracted more and more attention due to their relative safety and small side effects. Many plant extracts contain multiple active ingredients, which can act on hair follicles through multiple pathways, regulate the growth cycle of hair follicles, and promote hair growth. Therefore, it is of great practical significance to develop an anti-hair loss composition based on natural ingredients, which is safe, effective and has a long-lasting effect. The present invention aims to provide an anti-hair loss composition containing Platycladus orientalis leaves, which can overcome the shortcomings of existing anti-hair loss products by rationally combining multiple active ingredients to exert a synergistic effect, so as to provide a better solution for patients with hair loss. Summary of the invention

[0005] In view of the above technical problems, the object of the present invention is to provide an anti-hair loss composition containing Platycladus orientalis leaves and a preparation method and application thereof. The anti-hair loss composition provided by the present invention achieves the effect of anti-hair loss by reasonably combining multiple active ingredients to exert a synergistic effect.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides an anti-hair loss composition containing Platycladus orientalis leaves. The composition comprises the following components in parts by weight: 15-25 parts of Platycladus orientalis leaf extract, 8-12 parts of ginseng root extract, 10-20 parts of Channa intestine extract, 10-20 parts of Polygonum multiflorum extract, 5-10 parts of tea seed extract, 5-10 parts of Gleditsia sinensis extract, 5-10 parts of mulberry bark extract, 3-8 parts of Dictamni root bark extract, 3-8 parts of Cnidium monnieri seed extract and 2-5 parts of mint extract.

[0008] Preferably, the Platycladus orientalis leaf extract is obtained by enzymatic hydrolysis with serrapeptase and nattokinase; and the Panax ginseng root extract is obtained by enzymatic hydrolysis with cellulase and snail protease in sequence.

[0009] Preferably, the Channa intestine extract is obtained by fermenting Bifidobacterium longum and Lactobacillus delbrueckii subsp. bulgaricus; and the Polygonum multiflorum extract is obtained by fermenting Bacillus licheniformis and Clostridium butyricum in sequence.

[0010] Preferably, the tea seed extract, mulberry bark extract, Dictamni root bark extract and Cnidium monnieri seed extract are obtained by extraction with ethanol solution.

[0011] Preferably, the Gleditsia sinensis extract is obtained by water extraction.

[0012] Preferably, the peppermint extract is obtained by distillation.

[0013] The present invention also provides a method for preparing the above-mentioned anti-hair loss composition, which is characterized in that: the Platycladus orientalis leaf extract, ginseng root extract, Channa intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia sinensis extract, mulberry bark extract, Dictamni root bark extract, Cnidium monnieri seed extract and mint extract are mixed in parts by weight, sterilized, and the anti-hair loss composition is obtained.

[0014] The present invention also provides application of the anti-hair loss composition in preparing an anti-hair loss product.

[0015] Preferably, the anti-hair loss product is a skin external preparation for topical application.

[0016] Preferably, the anti-hair loss product can inhibit the activity of 5α-reductase and alleviate seborrheic alopecia.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention provides an anti-hair loss composition containing Platycladus orientalis leaves, comprising: Platycladus orientalis leaf extract, ginseng root extract, Channa intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia sinensis extract, mulberry bark extract, Dictamni root bark extract, Cnidium monnieri seed extract and mint extract. The Platycladus orientalis leaf extract of the present invention can regulate the hair follicle cycle and promote the hair follicle to enter the growth phase from the resting phase; the ginseng root extract stimulates the proliferation of dermal papilla cells of the hair follicles, enhances cell vitality, and regulates the expression of proteins related to the hair follicle cycle. The two cooperate to regulate the hair follicle cycle from different links and jointly promote hair regeneration. The Polygonum multiflorum extract of the present invention is rich in various nutrients, can nourish hair, and improve the color and toughness of hair; the Channa intestine extract also provides nutrients for hair, and the two act synergistically to nourish hair in all directions, enhance the health of hair, and reduce hair loss caused by malnutrition. The surface active ingredients in the tea seed extract and the Chinese honey locust extract of the present invention can clean the scalp grease and dirt and keep the hair follicles unobstructed; the mulberry bark extract regulates the scalp water metabolism, jointly improves the greasy environment of the scalp, prevents the grease from clogging the hair follicles, and creates a good basic condition for hair growth. The Dictamni root bark extract of the present invention has the effects of clearing away heat and dampness, dispelling wind and detoxifying, and can relieve scalp inflammation; the Cnidium monnieri seed extract can improve the cold and damp state of the scalp and also has a certain inhibitory effect on scalp inflammation. They work synergistically to reduce the damage of inflammation to the hair follicles and create a healthy scalp microenvironment for the normal growth of the hair follicles. The flavonoids in the Platycladus orientalis leaves of the present invention, the ginsenosides in ginseng, the anthraquinones in Polygonum multiflorum, etc., have synergistic effects in anti-oxidation, promoting blood circulation, regulating immunity, etc., can reduce the damage of free radicals to hair follicle cells, maintain the normal function of hair follicle cells, and jointly enhance the improvement effect on the problem of hair loss. The components of the invention have a synergistic effect, and have the functions of improving the oily environment of the scalp, preventing oil from clogging hair follicles, reducing the damage of inflammation to hair follicles, inhibiting the activity of 5α-reductase, improving the color and toughness of hair, relieving seborrheic alopecia, and achieving an anti-hair loss effect. DETAILED DESCRIPTION

[0019] The invention provides an anti-hair loss composition containing Platycladus orientalis leaves. The composition comprises the following components in parts by weight: 15-25 parts of Platycladus orientalis leaf extract, 8-12 parts of ginseng root extract, 10-20 parts of Channa intestine extract, 10-20 parts of Polygonum multiflorum extract, 5-10 parts of tea seed extract, 5-10 parts of Gleditsia sinensis extract, 5-10 parts of mulberry bark extract, 3-8 parts of Dictamni root bark extract, 3-8 parts of Cnidium monnieri seed extract and 2-5 parts of mint extract.

[0020] The Platycladus orientalis leaf extract of the present invention is preferably obtained by enzymolysis of serrapeptase and nattokinase: the dried Platycladus orientalis leaf is crushed through a 100-200 mesh sieve, mixed with water according to a solid-liquid ratio of 1:8-12g / ml, 0.3%-0.5% of the weight of the Platycladus orientalis leaf serrapeptase (enzyme activity of 20,000-50,000 U / g) and 0.1%-0.4% of the weight of the Platycladus orientalis leaf nattokinase (enzyme activity of 50,000-100,000 U / g) are added, and enzymolysis is carried out at 40-50°C and pH 7.5-8 for 1-3h, filtered, and the filtrate obtained is the Platycladus orientalis leaf extract. The Platycladus orientalis leaf extract obtained by the extraction method of the present invention improves scalp blood circulation, regulates the physiological function of hair follicles, and promotes hair follicles to enter the growth phase from the resting phase, which plays a key role in promoting hair growth.

[0021] The ginseng root extract of the present invention is preferably obtained by enzymolysis of cellulase and snail protease in sequence: the dried ginseng root is crushed through a 100-200 mesh sieve, mixed with water according to a solid-liquid ratio of 1:10-15g / ml, 0.4%-0.8% of the weight of the ginseng root is added with cellulase (enzyme activity is 100,000-200,000 U / g), enzymolysis is carried out at 45-55°C and pH 4.5-5.5 for 3-5h, 0.3%-0.5% of the weight of the ginseng root is added with snail protease (enzyme activity is 400,000-500,000 U / g), enzymolysis is carried out at 33-37°C and pH 5.5-7 for 1-3h, filtered, and the filtrate obtained is the ginseng root extract. The ginseng root extract obtained by the extraction method of the present invention can provide sufficient nutrition for the scalp and hair follicles, promote cell metabolism, enhance hair follicle vitality, better promote hair growth, and enhance the effect of hair growth.

[0022] The snakehead intestine extract of the present invention is preferably obtained by fermentation with Bifidobacterium longum and Lactobacillus delbrueckii subspecies Bulgaria: the dried snakehead intestine is crushed through an 80-100 mesh sieve, mixed with water according to a solid-liquid ratio of 1:12-15g / ml, inoculated with 1%-3% of the weight of the snakehead intestine Bifidobacterium longum (CCTCC NO: M 2019028, bacterial activity is 1.3 billion-1.8 billion CFU / g) and 0.8%-1.5% of the weight of the snakehead intestine Lactobacillus delbrueckii subspecies Bulgaria (CCTCC NO: M 2019203, bacterial activity is 2 billion-3 billion CFU / g) Fermented at 33-38°C, pH 6.2-7 for 2-4d, filtered with a 0.22-0.45μm filter membrane, sterilized, and the filtrate obtained is the snakehead intestine extract. The snakehead intestine extract obtained by the extraction method of the present invention nourishes the liver and kidneys, replenishes essence and blood, and improves hair loss.

[0023] The extract of Polygonum multiflorum of the present invention is preferably obtained by fermenting Bacillus licheniformis and Clostridium butyricum in sequence: the dried Polygonum multiflorum is crushed through an 80-100 mesh sieve, mixed with water at a solid-liquid ratio of 1:15-20 g / ml, and inoculated with 0.5%-1.5% of the weight of Polygonum multiflorum with Bacillus licheniformis 10103, bacterial activity of 2 billion to 4 billion CFU / g), aerobic fermentation at 35-40 ° C, pH 6.5-7 for 1-3 days, inoculated with 1% to 3% of the weight of Polygonum multiflorum Clostridium butyricum 24854, bacterial activity of 300 million-800 million CFU / g) is fermented at 33-38°C and pH 5.5-6.5 for 2-4 days, filtered with a 0.22-0.45 μm filter membrane, sterilized, and the filtrate obtained is the Polygonum multiflorum extract. The Polygonum multiflorum extract obtained by the extraction method of the present invention contains a variety of nutrients that can nourish hair, improve hair color and toughness, and reduce hair loss caused by malnutrition.

[0024] The tea seed extract of the present invention is preferably obtained by extraction with an ethanol solution: the dried tea seeds are crushed through a 100-200 mesh sieve, mixed with an ethanol solution with a volume percentage of 55%-65% at a solid-liquid ratio of 1:7-13g / ml, extracted for 30-60min at a temperature of 45-55°C and an ultrasonic power of 150-250W, filtered, and the filtrate is the tea seed extract. The tea seed extract obtained by the extraction method of the present invention can remove scalp oil and dirt, keep hair follicles unobstructed, and reduce the adverse effects of excessive scalp oil on hair follicles.

[0025] The mulberry bark extract of the present invention is preferably obtained by extraction with an ethanol solution: the dried mulberry bark is crushed through a 100-200 mesh sieve, mixed with an ethanol solution with a volume percentage of 45%-55% at a solid-liquid ratio of 1:5-10g / ml, extracted for 20-30min at a temperature of 55-65°C and an ultrasonic power of 250-350W, filtered, and the filtrate is the mulberry bark extract. The mulberry bark extract obtained by the extraction method of the present invention can regulate the water metabolism of the scalp and improve the scalp environment.

[0026] The Dictamni root bark extract of the present invention is preferably obtained by extraction with an ethanol solution: the dried Dictamni root bark is crushed through a 100-200 mesh sieve, mixed with an ethanol solution with a volume percentage of 70%-80% according to a solid-liquid ratio of 1:8-12g / ml, extracted for 25-35min at a temperature of 65-75°C and an ultrasonic power of 200-300W, filtered, and the filtrate is the Dictamni root bark extract. The Dictamni root bark extract obtained by the extraction method of the present invention can prevent scalp hair follicle inflammation caused by dampness, heat toxins, etc., and avoid hair loss caused by damage to hair follicles caused by inflammation.

[0027] The Cnidium monnieri seed extract of the present invention is preferably obtained by extraction with an ethanol solution: the dried Cnidium monnieri seeds are crushed through a 100-200 mesh sieve, mixed with an ethanol solution having a volume percentage of 60%-70% at a solid-liquid ratio of 1:6-10 g / ml, extracted for 20-40 minutes at a temperature of 60-70°C and an ultrasonic power of 150-250 W, filtered, and the filtrate is the Cnidium monnieri seed extract. The Cnidium monnieri seed extract obtained by the extraction method of the present invention can improve the cold and damp state of the scalp, promote blood circulation of the scalp, provide sufficient nutrition for hair follicles, and promote the repair and regeneration of hair follicle cells.

[0028] The sapodilla extract of the present invention is preferably obtained by water extraction: the dried sapodilla is crushed through an 80-100 mesh sieve, mixed with water at a solid-liquid ratio of 1:13-17 g / ml, stirred and extracted at 85-95° C. for 10-20 min, filtered, and the filtrate is the sapodilla extract. The sapodilla extract obtained by the extraction method of the present invention can remove grease and dirt from the scalp, inhibit the growth of microorganisms, reduce the incidence of scalp inflammation, reduce the damage of oxidative stress to hair follicle cells, protect the normal function of hair follicle cells, and enhance the strength and toughness of hair, and reduce hair breakage and shedding.

[0029] The mint extract of the present invention is preferably obtained by distillation: dried mint is crushed through a 20-60 mesh sieve, mixed with water at a solid-liquid ratio of 1:6-10 g / ml, soaked for 2-4 hours, and then distilled and extracted at a heating temperature of 102-110° C. for 3-5 hours, and the distillate is collected as the mint extract. The mint extract obtained by the extraction method of the present invention can lead the efficacy of other drugs to the head, promote the drugs to reach the hair follicles, better play a role in the scalp and hair follicles, and improve the anti-hair loss effect.

[0030] The present invention also provides a method for preparing the above-mentioned anti-hair loss composition, which is characterized in that: the Platycladus orientalis leaf extract, ginseng root extract, Channa intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia sinensis extract, mulberry bark extract, Dictamni root bark extract, Cnidium monnieri seed extract and mint extract are mixed in parts by weight, sterilized, and the anti-hair loss composition is obtained.

[0031] The present invention also provides application of the anti-hair loss composition in preparing an anti-hair loss product.

[0032] The anti-hair loss product of the present invention is a skin external preparation for local application. The anti-hair loss product comprises the above anti-hair loss composition and an additive or carrier acceptable in cosmetics / pharmaceuticals, wherein the additive or carrier may be a preservative, an antioxidant, an emulsifier, a moisturizer, a lubricant, a thickener, a penetration enhancer, a surfactant and an excipient.

[0033] The anti-hair loss product of the invention can inhibit the activity of 5α-reductase and alleviate seborrheic alopecia.

[0034] The method for using the anti-hair loss composition of the present invention is as follows: after washing and drying the hair, dilute the anti-hair loss composition with water by 4-6 times, spray it evenly on the hair loss part of the scalp in the morning and evening, and after applying, gently massage the scalp with the fingertips for 5 minutes.

[0035] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0037] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0038] Example 1

[0039] Preparation of anti-hair loss composition containing Platycladus orientalis leaves

[0040] (1) Preparation of each extract

[0041] Platycladus orientalis leaf extract: the dried Platycladus orientalis leaves were crushed through a 150-mesh sieve, mixed with water at a solid-liquid ratio of 1:10 g / ml, 0.4% of the weight of the Platycladus orientalis leaf serrapeptase (enzyme activity of 30,000 U / g) and 0.3% of the weight of the Platycladus orientalis leaf nattokinase (enzyme activity of 80,000 U / g) were added, and enzymolysis was performed at 45°C and pH 7.8 for 2h, the temperature was raised to 85°C for 15min to inactivate the enzyme, and the filtrate was filtered to obtain the Platycladus orientalis leaf extract;

[0042] Ginseng root extract: the dried ginseng root was crushed through a 150-mesh sieve, mixed with water at a solid-liquid ratio of 1:13 g / ml, 0.6% of the weight of the ginseng root cellulase (enzyme activity of 150,000 U / g) was added, and enzymolysis was performed at 50°C and pH 5 for 4 hours, 0.4% of the weight of the ginseng root snail protease (enzyme activity of 450,000 U / g) was added, and enzymolysis was performed at 35°C and pH 6 for 3 hours, the temperature was raised to 85°C for 15 minutes to inactivate the enzyme, and filtered to obtain the filtrate as the ginseng root extract;

[0043] Channa intestine extract: The dried Channa intestine was crushed and passed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:14 g / ml, inoculated with 2% of the weight of the Channa intestine Bifidobacterium longum (CCTCC NO: M 2019028, with a bacterial activity of 1.5 billion CFU / g) and 1% of the weight of the Channa intestine Lactobacillus delbrueckii subspecies bulgaricus (CCTCC NO: M 2019203, with a bacterial activity of 2.5 billion CFU / g), and fermented at 37° C. and pH 6.5 for 3 days, and the fermentation broth was filtered with a 0.22 μm filter membrane for sterilization to obtain the filtrate as the Channa intestine extract;

[0044] Polygonum multiflorum extract: dried Polygonum multiflorum was crushed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:17 g / ml, and inoculated with 1% of the weight of Polygonum multiflorum with Bacillus licheniformis 10103, bacterial activity of 3 billion CFU / g), after aerobic fermentation at 37°C and pH 6.7 for 2 days, inoculated with Clostridium butyricum at 2% of the weight of Polygonum multiflorum 24854, bacterial activity of 500 million CFU / g) was anaerobically fermented at 37°C and pH 6 for 3 days, and the fermentation broth was filtered with a 0.22 μm filter membrane for sterilization, and the filtrate obtained was the Polygonum multiflorum extract.

[0045] Tea seed extract: the dried tea seeds were crushed through a 150-mesh sieve, mixed with a 60% by volume ethanol solution at a solid-liquid ratio of 1:10 g / ml, extracted at 50° C. and 200 W ultrasonic power for 50 min, filtered, and the filtrate was the tea seed extract;

[0046] Mulberry bark extract: the dried mulberry bark is crushed and passed through a 150-mesh sieve, mixed with a 50% ethanol solution by volume at a solid-liquid ratio of 1:8 g / ml, extracted at 60° C. and 300 W ultrasonic power for 25 min, filtered, and the filtrate is the mulberry bark extract;

[0047] Dictamni root bark extract: the dried Dictamni root bark was crushed through a 150-mesh sieve, mixed with a 75% by volume ethanol solution at a solid-liquid ratio of 1:10 g / ml, extracted at 70°C and 250W ultrasonic power for 30 minutes, filtered, and the filtrate was the Dictamni root bark extract;

[0048] Cnidium monnieri seed extract: the dried cnidium monnieri seeds were crushed through a 150-mesh sieve, mixed with an ethanol solution having a volume percentage of 65% at a solid-liquid ratio of 1:8 g / ml, extracted at 65° C. and 200 W ultrasonic power for 30 min, filtered, and the filtrate was the cnidium monnieri seed extract;

[0049] Gleditsia sinensis extract: the dried Gleditsia sinensis was crushed through a 90-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / ml, stirred and extracted at 90° C. and 60 rpm for 15 min, filtered, and the filtrate was the Gleditsia sinensis extract;

[0050] Peppermint extract: crush dried peppermint through a 50-mesh sieve, mix with water at a solid-liquid ratio of 1:8 g / ml, soak for 3 hours, and then distill and extract at a heating temperature of 105° C. for 4 hours, and collect the distillate as the peppermint extract;

[0051] (2) Preparation of anti-hair loss composition

[0052] The following weight portions were weighed: 20 parts by weight of Platycladus orientalis leaf extract, 10 parts by weight of Panax ginseng root extract, 15 parts by weight of Channa intestine extract, 15 parts by weight of Polygonum multiflorum extract, 8 parts by weight of Camellia seed extract, 8 parts by weight of Gleditsia sinensis extract, 8 parts by weight of Morus alba bark extract, 5 parts by weight of Dictamni root bark extract, 5 parts by weight of Cnidium monnieri seed extract and 3 parts by weight of peppermint extract. The components were mixed and stirred at 200 r / min for 10 min, sterilized, and an anti-hair loss composition was obtained.

[0053] Example 2

[0054] Preparation of anti-hair loss composition containing Platycladus orientalis leaves

[0055] (1) Preparation of each extract

[0056] Platycladus orientalis leaf extract: the dried Platycladus orientalis leaves were crushed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:8 g / ml, 0.3% of serrapeptase (enzyme activity of 20,000 U / g) by weight of the Platycladus orientalis leaves and 0.1% of nattokinase (enzyme activity of 50,000 U / g) by weight of the Platycladus orientalis leaves were added, and enzymolysis was performed at 40°C and pH 7.5 for 3 hours, the temperature was raised to 90°C to inactivate the enzyme for 10 minutes, and the filtrate was filtered to obtain the Platycladus orientalis leaf extract;

[0057] Ginseng root extract: crush the dried ginseng root through a 100-mesh sieve, mix with water at a solid-liquid ratio of 1:10 g / ml, add 0.4% of the weight of the ginseng root cellulase (enzyme activity of 100,000 U / g), enzymolyze for 5 hours at 45°C and pH 4.5, add 0.3% of the weight of the ginseng root snail protease (enzyme activity of 400,000 U / g), enzymolyze for 3 hours at 33°C and pH 5.5, heat to 90°C to inactivate the enzyme for 10 minutes, filter, and the filtrate obtained is the ginseng root extract;

[0058] Channa intestine extract: The dried Channa intestine was crushed and passed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:12 g / ml, inoculated with 1% of the weight of the Channa intestine Bifidobacterium longum (CCTCC NO: M 2019028, with a bacterial activity of 1.3 billion CFU / g) and 0.8% of the weight of the Channa intestine Lactobacillus delbrueckii subspecies bulgaricus (CCTCC NO: M 2019203, with a bacterial activity of 2 billion CFU / g), and fermented at 33° C. and pH 6.2 for 4 days, and the fermentation broth was filtered with a 0.22 μm filter membrane for sterilization to obtain the filtrate as the Channa intestine extract;

[0059] Polygonum multiflorum extract: dried Polygonum multiflorum was crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / ml, and inoculated with 0.5% of the weight of Polygonum multiflorum with Bacillus licheniformis 10103, bacterial activity of 2 billion CFU / g), after aerobic fermentation at 35°C and pH 6.5 for 3 days, inoculated with 1% of the weight of Polygonum multiflorum, Clostridium butyricum 24854, bacterial activity of 300 million CFU / g) was fermented at 33°C and pH 5.5 for 4 days, and the fermentation broth was filtered with a 0.22 μm filter membrane for sterilization, and the filtrate obtained was the Polygonum multiflorum extract;

[0060] Tea seed extract: the dried tea seeds were crushed through a 100-mesh sieve, mixed with a 55% by volume ethanol solution at a solid-liquid ratio of 1:7 g / ml, extracted at 45° C. and 150 W ultrasonic power for 60 min, filtered, and the filtrate was the tea seed extract;

[0061] Mulberry bark extract: the dried mulberry bark is crushed and passed through a 100-mesh sieve, mixed with a 45% by volume ethanol solution at a solid-liquid ratio of 1:5 g / ml, extracted at 55° C. and 250 W ultrasonic power for 30 min, filtered, and the filtrate is the mulberry bark extract;

[0062] Dictamni root bark extract: the dried Dictamni root bark was crushed through a 100-mesh sieve, mixed with a 70% ethanol solution by volume at a solid-liquid ratio of 1:8 g / ml, extracted at 65° C. and 200 W ultrasonic power for 35 min, filtered, and the filtrate was the Dictamni root bark extract;

[0063] Cnidium monnieri seed extract: the dried cnidium monnieri seeds were crushed through a 100-mesh sieve, mixed with a 60% by volume ethanol solution at a solid-liquid ratio of 1:6 g / ml, extracted at 60° C. and 150 W ultrasonic power for 40 min, filtered, and the filtrate was the cnidium monnieri seed extract;

[0064] Gleditsia sinensis extract: the dried Gleditsia sinensis was crushed through an 80-mesh sieve, mixed with water at a solid-liquid ratio of 1:13 g / ml, stirred and extracted at 70 rpm at 85° C. for 20 min, filtered, and the filtrate was the Gleditsia sinensis extract;

[0065] Peppermint extract: crush dried peppermint through a 20-mesh sieve, mix with water at a solid-liquid ratio of 1:6 g / ml, soak for 4 hours, heat and distill at 102° C. for 5 hours, and collect the distillate as the peppermint extract;

[0066] (2) Preparation of anti-hair loss composition

[0067] The following weight portions were weighed: 15 parts by weight of Platycladus orientalis leaf extract, 8 parts by weight of Panax ginseng root extract, 10 parts by weight of Channa intestine extract, 10 parts by weight of Polygonum multiflorum extract, 5 parts by weight of Camellia seed extract, 5 parts by weight of Gleditsia sinensis extract, 5 parts by weight of Morus alba bark extract, 3 parts by weight of Dictamni root bark extract, 3 parts by weight of Cnidium monnieri seed extract and 2 parts by weight of peppermint extract. The components were mixed and stirred at 150 r / min for 15 min, sterilized, and an anti-hair loss composition was obtained.

[0068] Example 3

[0069] Preparation of anti-hair loss composition containing Platycladus orientalis leaves

[0070] (1) Preparation of each extract

[0071] Platycladus orientalis leaf extract: the dried Platycladus orientalis leaves were crushed through a 200-mesh sieve, mixed with water at a solid-liquid ratio of 1:12 g / ml, 0.5% of the weight of the Platycladus orientalis leaf serrapeptase (enzyme activity of 50,000 U / g) and 0.4% of the weight of the Platycladus orientalis leaf nattokinase (enzyme activity of 100,000 U / g) were added, and enzymolysis was performed at 50°C and pH 8 for 1 hour, the temperature was raised to 80°C to inactivate the enzyme for 20 minutes, and the filtrate was filtered to obtain the Platycladus orientalis leaf extract;

[0072] Ginseng root extract: crush the dried ginseng root through a 200-mesh sieve, mix with water at a solid-liquid ratio of 1:15 g / ml, add 0.8% of the weight of the ginseng root cellulase (enzyme activity of 200,000 U / g), enzymolyze for 3 hours at 55°C and pH 5.5, add 0.5% of the weight of the ginseng root snail protease (enzyme activity of 500,000 U / g), enzymolyze for 1 hour at 37°C and pH 7, heat to 80°C to inactivate the enzyme for 20 minutes, filter, and the filtrate obtained is the ginseng root extract;

[0073] Channa intestine extract: The dried Channa intestine was crushed and passed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:15 g / ml, inoculated with 3% of the weight of the Channa intestine Bifidobacterium longum (CCTCC NO: M 2019028, with a bacterial activity of 1.8 billion CFU / g) and 1.5% of the weight of the Channa intestine Lactobacillus delbrueckii subsp. bulgaricus (CCTCC NO: M 2019203, with a bacterial activity of 3 billion CFU / g), and fermented at 38° C. and pH 7 for 2 days, and the fermentation broth was filtered with a 0.45 μm filter membrane for sterilization to obtain the filtrate as the Channa intestine extract;

[0074] Polygonum multiflorum extract: The dried Polygonum multiflorum was crushed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:20 g / ml, and inoculated with 1.5% of the weight of Polygonum multiflorum with Bacillus licheniformis. 10103, bacterial activity of 4 billion CFU / g), after aerobic fermentation at 40°C and pH 7 for 1 day, inoculated with 3% of the weight of Polygonum multiflorum, Clostridium butyricum 24854, bacterial activity of 800 million CFU / g) was anaerobically fermented at 38°C and pH 6.5 for 2 days, and the fermentation broth was filtered with a 0.45 μm filter membrane for sterilization, and the obtained filtrate was the Polygonum multiflorum extract;

[0075] Tea seed extract: the dried tea seeds were crushed through a 200-mesh sieve, mixed with an ethanol solution having a volume percentage of 65% at a solid-liquid ratio of 1:13 g / ml, extracted at 55° C. and 250 W ultrasonic power for 30 min, filtered, and the filtrate was the tea seed extract;

[0076] Mulberry bark extract: the dried mulberry bark is crushed and passed through a 200-mesh sieve, mixed with a 55% by volume ethanol solution at a solid-liquid ratio of 1:10 g / ml, extracted at 65° C. and 350 W ultrasonic power for 20 min, filtered, and the filtrate is the mulberry bark extract;

[0077] Dictamni root bark extract: the dried Dictamni root bark was crushed through a 200-mesh sieve, mixed with an ethanol solution having a volume percentage of 80% at a solid-liquid ratio of 1:12 g / ml, extracted at 75°C and 300 W ultrasonic power for 25 min, filtered, and the filtrate was the Dictamni root bark extract;

[0078] Cnidium monnieri seed extract: the dried cnidium monnieri seeds were crushed through a 200-mesh sieve, mixed with an ethanol solution having a volume percentage of 70% at a solid-liquid ratio of 1:10 g / ml, extracted at 70° C. and 250 W ultrasonic power for 20 min, filtered, and the filtrate was the cnidium monnieri seed extract;

[0079] Gleditsia sinensis extract: the dried Gleditsia sinensis was crushed through a 100-mesh sieve, mixed with water at a solid-liquid ratio of 1:17 g / ml, extracted at 95° C. for 10 min, filtered, and the filtrate was the Gleditsia sinensis extract;

[0080] Peppermint extract: crush dried peppermint through a 60-mesh sieve, mix with water at a solid-liquid ratio of 1:10 g / ml, soak for 4 hours, and then distill and extract at a heating temperature of 110° C. for 3 hours, and collect the distillate as the peppermint extract;

[0081] (2) Preparation of anti-hair loss composition

[0082] The following weight portions are weighed: 25 parts by weight of Platycladus orientalis leaf extract, 12 parts by weight of Panax ginseng root extract, 20 parts by weight of Channa intestine extract, 20 parts by weight of Polygonum multiflorum extract, 10 parts by weight of Camellia seed extract, 10 parts by weight of Gleditsia sinensis extract, 10 parts by weight of Morus alba bark extract, 8 parts by weight of Dictamni root bark extract, 8 parts by weight of Cnidium monnieri seed extract and 5 parts by weight of peppermint extract, and the components are mixed and stirred at 250 r / min for 8 minutes, sterilized, and an anti-hair loss composition is obtained.

[0083] Comparative Example 1

[0084] The specific implementation method is the same as that of Example 1, except that when preparing the Platycladus orientalis leaf extract, serrapeptase and nattokinase are replaced by trypsin with an enzyme activity of 30,000 U / g and bromelain with an enzyme activity of 80,000 U / g, and enzymolysis is performed at 40° C. and pH 7.8 for 2 hours.

[0085] Comparative Example 2

[0086] The specific implementation method is the same as that of Example 1, except that the enzymatic hydrolysis order of cellulase and snail protease is switched when preparing the ginseng root extract.

[0087] Comparative Example 3

[0088] The specific implementation method is the same as that of Example 1, except that when preparing the snakehead intestine extract, Bifidobacterium longum is replaced by Bifidobacterium longum BB536 with a bacterial activity of 1.5 billion CFU / g, and the inoculation amount is 2% of the weight of the snakehead intestine; Lactobacillus delbrueckii subsp. bulgaricus is replaced by Lactobacillus helveticus with a bacterial activity of 2.5 billion CFU / g 6024), the inoculum size was 1% of the weight of the snakehead intestine, and the fermentation conditions remained unchanged.

[0089] Comparative Example 4

[0090] The specific implementation method is the same as that of Example 1, except that when preparing the Polygonum multiflorum extract, the fermentation order of Bacillus licheniformis and Clostridium butyricum is exchanged.

[0091] Comparative Example 5

[0092] The specific implementation method is the same as that of Example 1, except that the Platycladus orientalis leaf extract is not added.

[0093] Comparative Example 6

[0094] The specific implementation is the same as that of Example 1, except that the Polygonum multiflorum extract is not added.

[0095] Comparative Example 7

[0096] The specific implementation method is the same as that of Example 1, except that no ginseng root extract is added.

[0097] Test Example 1

[0098] 5α-reductase activity

[0099] When the activity of 5α-reductase is strong, more testosterone will be converted into DHT, stimulating the sebaceous glands to over-secrete sebum. The activity of 5α-reductase is inhibited, and the sebaceous glands reduce the secretion of sebum. This experiment tests the anti-hair loss compositions of Examples 1-3 and Comparative Examples 1-7 to reflect their oil control effects.

[0100] The anti-hair loss compositions of Examples 1-3 and Comparative Examples 1-7 were diluted 5 times with deionized water respectively.

[0101] The negative control was PBS solution, and the positive control was finasteride, which was diluted with anhydrous ethanol to a concentration of 0.1% by mass.

[0102] Dilutions of the anti-hair loss compositions of Examples 1-3 and Comparative Examples 1-7, negative control groups, positive control groups and blank tube groups were set up respectively, and different reagent solutions (5α-reductase crude enzyme + Tris-HCl buffer at pH 5.5 + testosterone + test solution + NADPH) were added to each group respectively, shaken well, and each group was subjected to HPLC analysis to determine the testosterone content of each tube. The change in the testosterone peak area at 0 min before the reaction and 30 min after the reaction was used to reflect the activity inhibition rate of the test solution in the system on 5α-reductase. The specific results are shown in Table 1.

[0103] 5α-reductase inhibition rate = (1-testosterone content change value of the test group / testosterone content change value of the blank group) × 100%.

[0104] Table 1 5α-reductase activity inhibition rate in each group

[0105]

[0106]

[0107] It can be seen from the data in Table 1 that the anti-hair loss composition obtained by the preparation method of Examples 1-3 of the present invention can significantly improve the 5α-reductase inhibition rate relative to the negative control, and achieve good anti-hair loss efficacy. It can be seen from the data of Example 1 and Comparative Examples 1-4 that changing the enzyme type, enzymolysis order, fermentation microorganism type and fermentation order has a great influence on the 5α-reductase inhibition rate. It can be seen from the data of Example 1 and Comparative Examples 5-7 that Platycladus orientalis leaf extract, ginseng root extract and Polygonum multiflorum extract have a greater influence on the 5α-reductase inhibition rate.

[0108] Test Example 2

[0109] The Hamilton grading system divides hair loss into 7 levels, each with different manifestations. The higher the level, the more severe the hair loss:

[0110] Grade I: The front hairline is normal, with only very slight receding of the temporal part;

[0111] Grade II: mild temporal receding with thinning of the hair at the frontal hairline;

[0112] Grade III: The amount of hair loss reaches the minimum standard of the definition of hair loss, with obvious hair retreat on the forehead, leaving only a small amount or almost no hair;

[0113] Grade III: Vertex type, hair loss is limited to the top of the head, and the hair on the forehead is obviously receding (the degree of receding is lighter than Grade II);

[0114] Grade IV: The hairline of the forehead and temples is obviously receding, the hair becomes thinner, and the hair on the top of the head is sparse, or even bald;

[0115] Grade V: Large bald areas on the front and top of the scalp, which are more severe than Grade IV. The junction between the top and the forehead is very narrow, with only sparse hair.

[0116] Grade VI: The junction between the top and the forehead disappears, and the baldness spreads to the sides and back;

[0117] Grade VI I: Only a narrow horseshoe-shaped hair growth zone remains on both sides of the scalp and the occipital region. This hair growth zone extends to the front of the ear in front and to the low position of the occipital region in the back. It is symmetrical on both sides, but the hair in front of the ear recedes backwards and downwards.

[0118] According to the Hamilton hair loss classification, 100 male subjects who met the Hamilton hair loss classification grades I I-IV were selected and evenly divided into two groups, one group used the anti-hair loss composition of Example 1 of the present invention, and the other group used minoxidil tincture. There was no significant difference in age and Hamilton hair loss classification between the two groups.

[0119] The method of using the anti-hair loss composition of Example 1 of the present invention is as follows: after washing and drying the hair, dilute the anti-hair loss composition of Example 1 with water 5 times, and evenly spray 1.2 ml of the diluted anti-hair loss composition on the hair loss part of the scalp. After spraying, gently massage the scalp with the fingertips for 5 minutes, once in the morning and once in the evening every day.

[0120] The method of using minoxidil tincture is: after washing and drying the hair, apply 1mL of minoxidil tincture to the hair loss area of ​​the scalp. After application, gently massage the scalp with your fingertips for 5 minutes, once in the morning and once in the evening.

[0121] Efficacy criteria:

[0122] Recovery: The hair growth in the hair loss area returns to normal, and the density and thickness of the hair are basically the same as before the hair loss;

[0123] Significantly effective: The number of hairs increases significantly, with the density increasing by more than 50%, or the hair diameter thickens by more than 50%, and more new terminal hair grows;

[0124] Effective: Hair loss is controlled to a certain extent, the thinning condition of hair is improved, the density increases by 15%-50%, or the hair diameter thickens by 15%-50%, and a certain amount of new vellus hair or a small amount of terminal hair grows;

[0125] Ineffective: There is no significant improvement in hair loss, the increase in hair density is not obvious, and a large amount of hair loss continues, or only a small amount of vellus hair grows and falls off quickly, and the overall appearance is basically the same as before treatment.

[0126] Total effective rate = (cured cases + markedly effective cases + effective cases) / 50×100%.

[0127] After 2 months of use, the efficacy of each group was statistically analyzed, as shown in Table 2.

[0128] Table 2 Statistics of the anti-hair loss effect in each group

[0129]

[0130]

[0131] It can be seen from the data in Table 2 that the anti-hair loss composition of the present invention is equivalent to minoxidil tincture in terms of anti-hair loss effect.

[0132] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An anti-hair loss composition containing Platycladus orientalis leaves, characterized in that: The invention comprises the following components in parts by weight: 15-25 parts of Platycladus orientalis leaf extract, 8-12 parts of ginseng root extract, 10-20 parts of Channa intestine extract, 10-20 parts of Polygonum multiflorum extract, 5-10 parts of tea seed extract, 5-10 parts of Gleditsia sinensis extract, 5-10 parts of mulberry bark extract, 3-8 parts of Dictamni root bark extract, 3-8 parts of Cnidium monnieri seed extract and 2-5 parts of peppermint extract.

2. The anti-hair loss composition according to claim 1, characterized in that The Platycladus orientalis leaf extract is obtained by enzymolysis of serrapeptase and nattokinase; and the Panax ginseng root extract is obtained by enzymolysis of cellulase and snail protease in sequence.

3. The anti-hair loss composition according to claim 1, characterized in that The snakehead intestine extract is obtained by fermenting Bifidobacterium longum and Lactobacillus delbrueckii subspecies bulgaricus; and the Polygonum multiflorum extract is obtained by fermenting Bacillus licheniformis and Clostridium butyricum in sequence.

4. The anti-hair loss composition according to claim 1, characterized in that The tea seed extract, mulberry bark extract, Dictamni root bark extract and Cnidium monnieri seed extract are obtained by extraction with ethanol solution.

5. The anti-hair loss composition according to claim 1, characterized in that The sapodilla extract is obtained by water extraction.

6. The anti-hair loss composition according to claim 1, characterized in that The peppermint extract is obtained by distillation.

7. The method for preparing the anti-hair loss composition according to any one of claims 1 to 6, characterized in that: include: The Platycladus orientalis leaf extract, ginseng root extract, Channa intestine extract, Polygonum multiflorum extract, tea seed extract, Gleditsia sinensis extract, Morus alba bark extract, Dictamni root bark extract, Cnidium monnieri seed extract and peppermint extract are mixed in parts by weight and sterilized to obtain the anti-hair loss composition.

8. Use of the anti-hair loss composition according to any one of claims 1 to 7 in the preparation of anti-hair loss products.

9. The use according to claim 8, characterized in that: The anti-hair loss product is an external preparation for skin for topical application.

10. The use according to claim 8, characterized in that: The anti-hair loss product can inhibit the activity of 5α-reductase and alleviate seborrheic alopecia.

Images