Application of atractylodes macrocephala in improving the content of fatty acid in eggs

Abstract

The application discloses application of white atractylodes rhizome in increasing content of fatty acids in eggs, and further discloses a method for increasing content of fatty acids in eggs, which is to feed laying hens with additives prepared from white atractylodes rhizome. The application can effectively increase content of fatty acids such as palmitic acid, palmitoleic acid, oleic acid, stearic acid, arachidic acid, alpha-linolenic acid and DHA in eggs by feeding laying hens with additives prepared from white atractylodes rhizome, but the change of content of cholesterols is not obvious, so that the additive is a very ideal nutritional additive, and provides a simple and convenient method for obtaining high-fatty-acid and low-cholesterol eggs.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of poultry breeding, in particular to the application of atractylodes in improving the content of fatty acids in eggs. BACKGROUND

[0002] Eggs are rich in protein and various trace elements, and are one of the common food materials. Eggs are rich in various essential fatty acids for the human body, and these fatty acids are mainly unsaturated fatty acids, including linoleic acid and linolenic acid which are beneficial to the human body, have a protective effect on the cardiovascular system, and have a positive influence on the development of the brain and nervous system.

[0003] In order to improve the content of fatty acids in eggs, palmitic acid, oleic acid and other nutrients or plant oils rich in these nutrients such as peanut oil, sunflower oil and linseed oil are often added to the basic feed of laying hens, but this method will increase the cost of breeding and cannot be widely used.

[0004] Therefore, the present application is proposed. SUMMARY

[0005] The purpose of the present application is to provide the application of atractylodes in improving the content of fatty acids in eggs and improve the nutritional value of eggs.

[0006] The technical scheme of the present application is described in detail as follows:

[0007] In the first aspect, the present application provides the application of atractylodes in improving the content of fatty acids in eggs, and the atractylodes is made into an additive to feed laying hens.

[0008] Atractylodes is warm in nature and belongs to the spleen and stomach channels. Atractylodes likes cool and mild climate, can resist cold and drought, and hates waterlogging. Atractylodes is the dried rhizome of Atractylodes lancea of the family Asteraceae, which is a commonly used medicine in clinical practice of traditional Chinese medicine. Atractylodes is often used in combination with angelica, astragalus, wind-preventing and radix scrophulariae, etc. and has the effects of invigorating the spleen, eliminating dampness, tonifying qi and blood, eliminating dampness and stopping diarrhea, promoting saliva and stopping thirst, etc. and has the effects of treating spontaneous sweating and fetal movement. The content of volatile oil in atractylodes is high, and there are also atractylodes polysaccharides, atractylodes lactones and other components. Atractylodes polysaccharides are composed of various sugars such as glucose, mannose, arabinose and galactose. Studies have shown that atractylodes polysaccharides have the effects of protecting the liver, improving the gastrointestinal tract, regulating immunity, anti-tumor activity, anti-inflammatory and antibacterial, reducing blood sugar, etc. and can inhibit the apoptosis of nerve cells and play a protective role on the nerves. The present application found that adding atractylodes to the feed of laying hens can effectively improve the content of fatty acids in eggs and improve the nutritional value of eggs.

[0009] In the second aspect, the present application provides a method for improving the content of fatty acids in eggs, which feeds laying hens with atractylodes made into an additive.

[0010] Optionally or preferably, the feed additive contains atractylodes rhizome powder and / or atractylodes rhizome polysaccharide. The atractylodes rhizome powder is obtained by crushing the dried rhizome of atractylodes. The atractylodes rhizome polysaccharide can be extracted by various existing extraction methods, or directly purchased from the market. The purity of the commercially available atractylodes rhizome polysaccharide is generally above 95%.

[0011] Optionally or preferably, the feeding amount of the atractylodes rhizome polysaccharide is 400 mg / kg, and the feeding amount of the atractylodes rhizome powder is 15 g / kg, calculated based on the body weight of the laying hens.

[0012] Optionally or preferably, the laying hens are Tianlu chickens.

[0013] Optionally or preferably, the fatty acids include at least one of palmitic acid, palmitoleic acid, oleic acid, stearic acid, alpha-linolenic acid, arachidonic acid and DHA.

[0014] Compared with the prior art, the present application has the following beneficial effects:

[0015] The present application feeds the laying hens with the atractylodes rhizome additive, which can effectively increase the content of fatty acids such as palmitic acid, palmitoleic acid, oleic acid, stearic acid, alpha-linolenic acid, arachidonic acid and DHA in the eggs, but the cholesterol content does not change significantly, so it is an ideal nutritional additive, and provides a simple and convenient method for obtaining high-fat and low-cholesterol eggs. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 The palmitic acid content statistical results of the eggs in each group of the examples are shown in Table 1.

[0017] Figure 2 The palmitoleic acid content statistical results of the eggs in each group of the examples are shown in Table 2.

[0018] Figure 3 The stearic acid content statistical results of the eggs in each group of the examples are shown in Table 3.

[0019] Figure 4 The oleic acid content statistical results of the eggs in each group of the examples are shown in Table 4.

[0020] Figure 5 The linoleic acid content statistical results of the eggs in each group of the examples are shown in Table 5.

[0021] Figure 6 The alpha-linolenic acid content statistical results of the eggs in each group of the examples are shown in Table 6.

[0022] Figure 7 The arachidonic acid content statistical results of the eggs in each group of the examples are shown in Table 7.

[0023] Figure 8 The DHA content statistical results of the eggs in each group of the examples are shown in Table 8. DETAILED DESCRIPTION

[0024] For those skilled in the art to better understand the present application, the present application will be clearly and completely described below in conjunction with the embodiments and drawings. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor should fall within the scope of protection of the present application. The instruments and reagents used in the embodiments are from commercial channels if not otherwise specified.

[0025] Example 1 Effect of Atractylodes on egg quality

[0026] 1 Test materials and animals

[0027] 1.1 Test materials

[0028] In this test, Zhejiang Atractylodes was selected, and Atractylodes polysaccharide was extracted by Yangling Ciyuan Biotechnology Co., Ltd. (purity 95%).

[0029] 1.2 Test animals

[0030] Healthy and similar weight 31-week-old Tianlu Qingma chicken No. 2 hens (one of Tianlu chicken breeds) were selected from Wens Group Gaocun Wens Poultry Farm.

[0031] 2 Animal feeding and treatment

[0032] 2.1 Experimental animal grouping

[0033] A single factor design test was used. In this experiment, Tianlu Qingma chicken No. 2 hens at the 4th week of egg production peak (31 weeks old) and with no significant difference in egg production rate were selected, and the test period was 4 weeks, from 31 weeks old to 34 weeks old.

[0034] The experiment was divided into 3 groups, namely A (Atractylodes powder) group, B (Atractylodes polysaccharide) group and C (control) group, 10 replicates were set in each group, 12 in each replicate, a total of 120 hens, and 360 hens in 3 groups.

[0035] 2.2 Grouped animal treatment

[0036] The control group of hens was fed with conventional feed, and the conventional procedure was used during the test period. The Atractylodes polysaccharide group of hens was fed with conventional feed + 400 mg / kg Atractylodes polysaccharide, and the Atractylodes powder group of hens was fed with conventional feed + 15 g / kg Atractylodes powder. Atractylodes polysaccharide and Atractylodes powder were added in the feed on the first day of the formal test, for a total of 3 weeks (21d). During the test period, the hens were fed quantitatively and drank water freely according to the requirements of egg production management, and the light was sufficient. The test animal grouping and treatment are shown in Table 1.

[0037] Table 1 Test grouping and treatment

[0038]

[0039] Atractylodes rhizome powder stirring method: according to the 405 chickens in a production line of the chicken farm, 47 kg of feed and 0.7 kg of Atractylodes rhizome are needed per day, and the 21 d requirement is 14.7 kg. After the Atractylodes rhizome is crushed into particles in the laboratory, it is packaged into 0.7 kg / bag of Atractylodes rhizome powder. One bag is taken every day and mixed evenly with 47 kg of conventional feed by a stirrer (the feed is mixed in advance the night before feeding). The required feed is obtained.

[0040] Atractylodes rhizome polysaccharide stirring method: according to the 405 chickens in a production line of the chicken farm, 47 kg of feed and 20 g of Atractylodes rhizome polysaccharide are needed per day, and the 21 d requirement is 420 g. It is pre-packaged into 20 g / bag in the laboratory. One bag is taken every day and dissolved in 600 mL of water, and then sprayed evenly on 5 kg of feed and mixed evenly. Then, 42 kg of feed is mixed by a stirrer (the feed needs to be mixed before feeding in the morning every day), and the required feed is obtained.

[0041] 2.3 Sample collection and processing

[0042] The sampling time is set to the 1st day of the 34th week, and 30 eggs are randomly selected from each group for detection of the contents of nutrients such as fatty acids, vitamin A and cholesterol in the eggs, and relevant data are obtained. The instruments used in this detection are high-performance liquid chromatograph and gas chromatograph.

[0043] 2.4 Data processing and statistical analysis

[0044] The test data are statistically analyzed and plotted using Graph Pad Prism 9.5.1 statistical software. The average values are compared by One-Way ANOVA, and multiple comparisons between groups are performed. The data are represented as "mean ± standard deviation (mean ± SD)", and different letters on the top of the column chart and in the table data indicate significant differences between groups (P<0.05), and the same letters indicate no significant difference between groups (P>0.05). P P

[0045] 3 Results and analysis

[0046] The detection results of the fatty acid content in the 34th week eggs are shown in Table 2.

[0047] Table 2 Detection results of the fatty acid content in the eggs after adding drugs (g / 100g)

[0048]

[0049] Table 1 statistical results show that compared with the control group, the contents of palmitic acid, palmitoleic acid, oleic acid and linoleic acid in the eggs of the Atractylodes rhizome powder group increased significantly (​​P <0.05), the contents of palmitic acid, palmitoleic acid, oleic acid, stearic acid, linoleic acid, a-linolenic acid, arachidonic acid and DHA in the atractylodes rhizome polysaccharide group were significantly increased (P<0.05) P P<0.05). It is shown that the addition of atractylodes rhizome powder or atractylodes rhizome polysaccharide in the feed has an effect on the deposition of the above-mentioned nutritional components in the eggs.

[0050] In the experiment, the unsaturated fatty acids including palmitoleic acid, oleic acid, linoleic acid, DHA, etc. are measured. Among them, the omega-3 polyunsaturated fatty acids (omega-3 PUFA) mainly include linolenic acid (ALA) and docosahexaenoic acid (DHA). The omega-6 polyunsaturated fatty acids include LA and AA.

[0051] The experimental results show that the contents of linoleic acid, palmitoleic acid, a-linolenic acid, arachidonic acid and DHA in the eggs of the atractylodes rhizome powder group and the atractylodes rhizome polysaccharide group at 34 weeks are significantly different from those of the control group (P<0.05). P P<0.05), and the contents of unsaturated fatty acids in the atractylodes rhizome polysaccharide group show an upward trend, while the contents of cholesterol in the eggs of the atractylodes rhizome powder group and the atractylodes rhizome polysaccharide group do not show obvious upward trend. It is shown that the addition of atractylodes rhizome powder or atractylodes rhizome polysaccharide in the feed of laying hens can effectively increase the content of polyunsaturated fatty acids in the eggs.

[0052] Since the human body has limited ability to synthesize polyunsaturated fatty acids, the main way to obtain polyunsaturated fatty acids is from food. The method provided by the application can effectively increase the content of polyunsaturated fatty acids in the eggs and the content of cholesterol does not change significantly, thereby improving the nutritional value of the eggs.

[0053] In the present document, specific examples are used to elaborate the inventive concept in detail. It should be noted that any obvious modifications, equivalent replacements or other improvements made by those skilled in the art without departing from the inventive concept should be included in the protection scope of the application.

Claims

1. The application of Atractylodes macrocephala in increasing the fatty acid content of eggs, characterized in that: The Atractylodes macrocephala is used to prepare an additive for feeding laying hens, wherein the additive contains Atractylodes macrocephala powder and / or Atractylodes macrocephala polysaccharide; calculated based on the weight of the laying hens, the feeding amount of Atractylodes macrocephala polysaccharide is 400 mg / kg, and the feeding amount of Atractylodes macrocephala powder is 15 g / kg; the fatty acids include palmitic acid, palmitoleic acid, oleic acid, stearic acid, arachidonic acid, α-linolenic acid and DHA.

2. The use according to claim 1, characterized in that The laying hens are Tianlu chickens.

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