A fluorescent diagnostic kit and application thereof

Abstract

The application discloses a fluorescent diagnostic kit and application thereof, and belongs to the field of medical treatment. The fluorescent diagnostic kit comprises a base, a top cover rotatably arranged on the top of the base, a control panel arranged at the middle position of the top of the top cover, a data processing box arranged at the middle position of the bottom end of the inside of the base, reagent boxes and water pumps arranged at the two sides of the bottom end of the inside of the base, a partition plate arranged at the middle position of one side of the inside of the base, an electric heating plate arranged at the middle position of the top of the partition plate, and a reagent disc arranged on the top of the electric heating plate. The fluorescent diagnostic kit is provided with the electric heating plate, the reagent boxes, the water pumps and the mist spray head. If the external environment temperature is low, the reagent disc can be heated through the electric heating plate. If the external environment temperature is high, the fluorescent reagent in the reagent box can be extracted and sprayed into the reagent disc through cooperation between the reagent boxes, the water pumps and the mist spray head, so that the cooling effect is achieved, and the temperature of the reagent disc can be controlled by the fluorescent diagnostic kit.

Description

Technical Field

[0001] This invention relates to the field of bioimaging technology, specifically to a fluorescent diagnostic reagent kit and its application. Background Technology

[0002] Optical imaging of diseases represents a highly dynamic and multidisciplinary research field. Over the past decade, it has attracted widespread research attention from scientists across various disciplines, including chemistry, materials science, biotechnology, nanotechnology, and biomedicine. Optical imaging probes and techniques hold promise for early cancer diagnosis and image-guided therapy, significantly impacting cancer treatment. In vivo fluorescence imaging of biological systems in the near-infrared II region (NIR-II, 1000-1700 nm) is at the forefront of optical imaging technology research and holds great promise due to its minimal autofluorescence and tissue scattering, deep tissue imaging capabilities, high spatial resolution, and high contrast. Furthermore, recent studies have shown that fluorophores emitted in the NIR-II region can significantly improve image quality and signal-to-noise ratio compared to the traditional near-infrared I region (NIR-I, 650-900 nm). Therefore, the development of novel NIR-II fluorophores and molecular probes for in vivo imaging applications is of paramount importance and has a direct impact on the biomedical field.

[0003] When performing fluorescent diagnostics on the test solution, the labeling effect of the fluorescent reagent is easily affected by the concentration of the fluorescent reagent and the temperature of the labeling environment. If the concentration of the fluorescent reagent is low, the fluorescent reagent cannot completely label the diseased cells in the test solution. Moreover, when the fluorescent reagent is used to label the test solution, if the temperature is low, the diffusion rate of the fluorescent reagent is slow and the time taken is long. If the temperature is high, the test solution is prone to necrosis or mold. Existing fluorescent diagnostic kits cannot control the temperature of the reagent tray. Summary of the Invention

[0004] The purpose of this invention is to provide a fluorescent diagnostic reagent kit and its application, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a fluorescent diagnostic reagent kit and its application, comprising a base, a top cover rotatably mounted on the top of the base, a touch screen mounted at the center of the top of the top cover, a data processing box mounted at the center of the bottom of the base, reagent boxes and water pumps mounted on both sides of the bottom of the base, a partition mounted at the center of one side of the base, an electric heating plate mounted at the center of the top of the partition, a reagent tray mounted on the top of the electric heating plate, a solid fluorescent reagent layer mounted at the bottom of the reagent tray, heat-conducting plates mounted on both sides of the top of the partition, a mist nozzle mounted at the top of the heat-conducting plate, mounting plates mounted on both sides of the top of the partition away from the electric heating plate, a contact temperature sensor mounted on the top of the partition via the mounting plates, a fluorescence measurement unit mounted at the center of the top of the top of the top cover, and ultraviolet lamps mounted on both sides of the inside of the top cover.

[0006] Preferably, liquid inlet holes are provided on both sides of one end of the base, the reagent box is connected to the outside through the liquid inlet holes, the input end of the water pump is connected to the reagent box, and the output end of the water pump is connected to the mist nozzle in the heat-conducting plate.

[0007] Preferably, the reagent tray is provided with multiple baffles, the reagent tray is divided into multiple detection areas by the multiple baffles, the multiple baffles on the reagent tray are provided with air vents, and the multiple detection areas on the reagent tray are connected by the air vents.

[0008] Preferably, a through hole is provided on the top of one side of the heat-conducting plate, the size of the through hole on the heat-conducting plate is adapted to the size of the mist nozzle, and the mist nozzle is connected to the heat-conducting plate through the through hole.

[0009] Preferably, a mounting hole is provided in the middle of one side of the mounting plate, and the size of the mounting hole on the mounting plate is adapted to the size of the contact temperature sensor. The contact temperature sensor is connected to the mounting plate through the mounting hole.

[0010] Preferably, a pivot is provided on one side of the top of the base, and the base is rotatably connected to the top cover through the pivot.

[0011] Preferably, a fixing groove is provided at the middle position of the top of the top cover, and the size of the fixing groove on the top cover is adapted to the size of the touch screen. The top cover is connected to the touch screen through the fixing groove.

[0012] Preferably, the reagent tray has a retaining frame at the bottom, the size of which is adapted to the size of the electric heating plate, and the reagent tray is engaged with the electric heating plate through the retaining frame. Auxiliary plates are provided on the top of both sides of the reagent tray.

[0013] Preferably, the top cover has mounting grooves on both sides inside, the size of the mounting grooves on the top cover is adapted to the size of the ultraviolet lamp, and the top cover is connected to the ultraviolet lamp through the mounting grooves.

[0014] Preferably, the application steps are as follows:

[0015] S1: Set the temperature inside the diagnostic kit via the touchscreen;

[0016] S2: Drop the analyte onto the fluorescent reagent layer;

[0017] S3: Place the reagent tray containing the test sample into the diagnostic kit;

[0018] S4: Activate the ultraviolet lamp inside the diagnostic kit to irradiate the reagent tray;

[0019] S5: And start the electric heating plate to heat the reagent tray;

[0020] S6: If the reagent tray temperature is too high, the water pump and mist nozzle will cool the reagent tray.

[0021] S7: Finally, the fluorescence intensity in the reagent tray is measured using the fluorescence measurement unit.

[0022] Compared with the prior art, the beneficial effects of the present invention are:

[0023] 1. This fluorescent diagnostic kit and its application: When performing fluorescent diagnosis on the test solution, if the concentration of the fluorescent reagent prepared by the staff is too low, it cannot completely label the diseased cells in the test solution. The solid fluorescent reagent layer can melt to increase the concentration of the fluorescent reagent.

[0024] 2. The fluorescent diagnostic reagent kit and its application are equipped with an electric heating plate, a reagent box, a water pump, and a mist nozzle. If the ambient temperature is low, the reagent tray can be heated by the electric heating plate. If the ambient temperature is high, the fluorescent reagent in the reagent box can be extracted and sprayed into the reagent tray through the cooperation of the reagent box, water pump, and mist nozzle to achieve a cooling effect, so that the fluorescent diagnostic reagent kit can control the temperature of the reagent tray. Attached Figure Description

[0025] Figure 1 This is a perspective view of the present invention;

[0026] Figure 2 This is the front view of the present invention;

[0027] Figure 3 This is a top view of the base of the present invention;

[0028] Figure 4 This is a front sectional view of the internal structure of the present invention;

[0029] Figure 5 For the present invention Figure 4 Enlarged view of the structure at point A in the middle;

[0030] Figure 6 This is a flowchart illustrating the application process of the diagnostic kit of the present invention.

[0031] In the diagram: 1. Base; 2. Top cover; 3. Touch screen; 4. Data processing box; 5. Reagent box; 6. Water pump; 7. Partition; 8. Electric heating plate; 9. Reagent tray; 10. Solid fluorescent reagent layer; 11. Heat-conducting plate; 12. Mist nozzle; 13. Mounting plate; 14. Contact temperature sensor; 15. Fluorescence measurement unit; 16. Ultraviolet lamp. Detailed Implementation

[0032] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0033] Please see Figure 1-6This invention provides an embodiment of a fluorescent diagnostic reagent kit and its application, comprising a base 1, a top cover 2 rotatably mounted on the top of the base 1, a touch screen 3 mounted in the middle of the top of the top cover 2, the touch screen 3 being electrically connected to a data processing box 4, a water pump 6, an electric heating plate 8, a contact temperature sensor 14, a fluorescence measurement unit 15, and an ultraviolet lamp 16, the data processing box 4 being mounted in the middle of the bottom of the base 1, the data processing box 4 cooperating with the fluorescence measurement unit 15 to perform fluorescence imaging of the test solution, reagent boxes 5 and water pumps 6 being mounted on both sides of the bottom of the base 1, a partition 7 being mounted in the middle of one side of the base 1, and an electric heating plate 8 being mounted in the middle of the top of the partition 7, the electric heating plate 8 heating the reagent tray 9 to avoid slow diffusion of fluorescent reagents due to low external temperature, the top of the electric heating plate 8... The reagent tray 9 is equipped with a solid fluorescent reagent layer 10 at the bottom. When the ambient temperature is low, the electric heating plate 8 can assist the solid fluorescent reagent layer 10 to melt. Heat-conducting plates 11 are installed on both sides of the top of the partition 7. The contact temperature sensor 14 is assisted by the heat-conducting plates 11 to sense the temperature of the reagent tray 9. A mist nozzle 12 is installed at the top inside the heat-conducting plates 11. Mounting plates 13 are installed on both sides of the top of the partition 7 away from the electric heating plate 8. The contact temperature sensor 14 is installed on the top of the partition 7 through the mounting plates 13. A fluorescence measurement unit 15 is installed in the middle of the top of the top inside the top of the top cover 2. The fluorescence measurement unit 15 can image and display the fluorescence marking of the test solution on the reagent tray 9. Ultraviolet lamps 16 are installed on both sides inside the top cover 2. The ultraviolet lamps 16 irradiate the fluorescent reagent and the test solution to assist in the fluorescence marking.

[0034] In this embodiment, liquid inlet holes are provided on both sides of one end of the base 1. The reagent tank 5 is connected to the outside through the liquid inlet holes. The input end of the water pump 6 is connected to the reagent tank 5, and the output end of the water pump 6 is connected to the mist nozzle 12 in the heat-conducting plate 11. The operator injects liquid fluorescent reagent into the reagent tank 5 through the liquid inlet holes. The liquid fluorescent reagent can be atomized by the cooperation between the water pump 6 and the mist nozzle 12 and sprayed into the reagent tray 9 to achieve a cooling effect.

[0035] In this embodiment, the reagent tray 9 is provided with multiple baffles, and the reagent tray 9 is divided into multiple detection areas by the multiple baffles. The multiple baffles on the reagent tray 9 are provided with air vents, and the multiple detection areas on the reagent tray 9 are connected by the air vents, so that the atomized liquid fluorescent reagent can enter each detection area through the air vents.

[0036] In this embodiment, a through hole is provided on the top of one side of the heat-conducting plate 11. The size of the through hole on the heat-conducting plate 11 is adapted to the size of the mist nozzle 12. The mist nozzle 12 is connected to the heat-conducting plate 11 through the through hole and is stably and firmly installed on the heat-conducting plate 11 through the through hole.

[0037] In this embodiment, a mounting hole is provided in the middle of one side of the mounting plate 13. The size of the mounting hole on the mounting plate 13 is adapted to the size of the contact temperature sensor 14. The contact temperature sensor 14 is connected to the mounting plate 13 through the mounting hole. The contact temperature sensor 14 can be stably installed on the mounting plate 13 through the mounting hole, and it is convenient for the staff to disassemble the faulty contact temperature sensor 14.

[0038] In this embodiment, a pivot is provided on one side of the top of the base 1. The base 1 is rotatably connected to the top cover 2 through the pivot, and the top cover 2 can rotate on the base 1 through the pivot.

[0039] In this embodiment, a fixing groove is provided in the middle of the top of the top cover 2. The size of the fixing groove on the top cover 2 is adapted to the size of the touch screen 3. The top cover 2 is connected to the touch screen 3 through the fixing groove, and the touch screen 3 is stably and firmly installed on the top cover 2 through the fixing groove.

[0040] In this embodiment, a retaining frame is provided at the bottom of the reagent tray 9. The size of the retaining frame on the reagent tray 9 is adapted to the size of the electric heating plate 8. The reagent tray 9 is connected to the electric heating plate 8 through the retaining frame. The reagent tray 9 can be placed stably and firmly on the electric heating plate 8 through the retaining frame. Auxiliary plates are provided on the top of both sides of the reagent tray 9. The staff can easily take the reagent tray 9 out of the base 1 through the auxiliary plates.

[0041] In this embodiment, mounting slots are provided on both sides inside the top cover 2. The size of the mounting slots on the top cover 2 is adapted to the size of the ultraviolet lamp 16. The top cover 2 is connected to the ultraviolet lamp 16 through the mounting slots, and the ultraviolet lamp 16 can be stably and firmly installed inside the top cover 2 through the mounting slots.

[0042] In this implementation, the application steps are as follows:

[0043] S1: Set the temperature inside the diagnostic kit via the touchscreen;

[0044] S2: Drop the analyte onto the fluorescent reagent layer;

[0045] S3: Place the reagent tray containing the test sample into the diagnostic kit;

[0046] S4: Activate the ultraviolet lamp inside the diagnostic kit to irradiate the reagent tray;

[0047] S5: And start the electric heating plate to heat the reagent tray;

[0048] S6: If the reagent tray temperature is too high, the water pump and mist nozzle will cool the reagent tray.

[0049] S7: Finally, the fluorescence intensity in the reagent tray is measured using the fluorescence measurement unit.

[0050] Example 1, as Figure 1-5 As shown, staff can set the temperature range within the diagnostic reagent kit via the touchscreen 3. When the liquid fluorescent reagent in the reagent tray 9 is used to label the test solution, the electric heating plate 8 can heat the reagent tray 9. The combination of the reagent box 5, water pump 6, and mist nozzle 12 allows the liquid fluorescent reagent to be atomized and cooled down the reagent tray 9. When the liquid fluorescent reagent is used for labeling, the ultraviolet lamp 16 can also be used for labeling.

[0051] Example 2, as Figure 6 As shown, S1: Set the temperature inside the diagnostic kit via the touchscreen; S2: Drop the analyte onto the fluorescent reagent layer; S3: Place the reagent tray containing the analyte inside the diagnostic kit; S4: Turn on the ultraviolet lamp inside the diagnostic kit to irradiate the reagent tray; S5: Turn on the electric heating plate to heat the reagent tray; S6: If the temperature of the reagent tray is too high, the water pump and mist nozzle will cool the reagent tray; S7: Finally, measure the fluorescence intensity inside the reagent tray using the fluorescence measurement unit.

[0052] For those skilled in the art, the present invention is not limited to the details of the exemplary embodiments described above, and the invention can be implemented in other specific forms without departing from the spirit or scope thereof. Therefore, the embodiments of the present invention are exemplary and not restrictive. The scope of the invention is defined by the appended claims rather than the foregoing description, and therefore all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0053] In the description of this invention, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" or "linked" should be interpreted broadly, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

Claims

1. A fluorescent diagnostic reagent kit, comprising a base (1), characterized in that: A top cover (2) is rotatably mounted on the top of the base (1). A touch screen (3) is mounted in the middle of the top of the top cover (2). A data processing box (4) is mounted in the middle of the bottom inside the base (1). A reagent box (5) and a water pump (6) are mounted on both sides of the bottom inside the base (1). A partition (7) is mounted in the middle of one side inside the base (1). An electric heating plate (8) is mounted in the middle of the top of the partition (7). A reagent tray (9) is mounted on the top of the electric heating plate (8). The reagent tray (9) is heated by the electric heating plate (8) to avoid the situation where the fluorescent reagent diffuses slowly due to the low external temperature. A solid fluorescent reagent layer (10) is installed at the bottom inside. When the outside temperature is low, the electric heating plate (8) can help the solid fluorescent reagent layer (10) melt. Heat-conducting plates (11) are installed on both sides of the top of the partition (7). A mist nozzle (12) is installed at the top inside the heat-conducting plate (11). Mounting plates (13) are installed on both sides of the top of the partition (7) away from the electric heating plate (8). A contact temperature sensor (14) is installed on the top of the partition (7) through the mounting plate (13). A fluorescence measurement unit (15) is installed in the middle of the top inside the top of the top cover (2). Ultraviolet lamps (16) are installed on both sides inside the top cover (2). The base (1) has liquid inlet holes on both sides at one end. The reagent box (5) is connected to the outside through the liquid inlet holes. The input end of the water pump (6) is connected to the reagent box (5). The output end of the water pump (6) is connected to the mist nozzle (12) in the heat-conducting plate (11). The reagent tray (9) is provided with multiple baffles, and the reagent tray (9) is divided into multiple detection areas by the multiple baffles. The multiple baffles on the reagent tray (9) are provided with air vents, and the multiple detection areas on the reagent tray (9) are connected by the air vents. A through hole is provided on the top of one side of the heat-conducting plate (11). The size of the through hole on the heat-conducting plate (11) is adapted to the size of the mist nozzle (12). The mist nozzle (12) is connected to the heat-conducting plate (11) through the through hole. A mounting hole is provided in the middle of one side of the mounting plate (13). The size of the mounting hole on the mounting plate (13) is adapted to the size of the contact temperature sensor (14). The contact temperature sensor (14) is connected to the mounting plate (13) through the mounting hole.

2. The fluorescent diagnostic reagent kit according to claim 1, characterized in that: A pivot is provided on one side of the top of the base (1), and the base (1) is rotatably connected to the top cover (2) through the pivot.

3. The fluorescent diagnostic reagent kit according to claim 1, characterized in that: A fixing groove is provided at the middle position of the top of the top cover (2). The size of the fixing groove on the top cover (2) is adapted to the size of the touch screen (3). The top cover (2) is connected to the touch screen (3) through the fixing groove.

4. The fluorescent diagnostic reagent kit according to claim 1, characterized in that: The reagent tray (9) is provided with a frame at the bottom. The size of the frame on the reagent tray (9) is adapted to the size of the electric heating plate (8). The reagent tray (9) is connected to the electric heating plate (8) through the frame. The top of both sides of the reagent tray (9) is provided with auxiliary plates.

5. A fluorescent diagnostic reagent kit according to claim 1, characterized in that: The top cover (2) has mounting slots on both sides inside. The size of the mounting slots on the top cover (2) is adapted to the size of the ultraviolet lamp (16). The top cover (2) is connected to the ultraviolet lamp (16) through the mounting slots.

6. The application of the fluorescent diagnostic reagent kit according to claim 1, characterized in that: The application steps are as follows: S1: Set the temperature inside the diagnostic kit via the touchscreen; S2: Drop the analyte onto the fluorescent reagent layer; S3: Place the reagent tray containing the test sample into the diagnostic kit; S4: Activate the ultraviolet lamp inside the diagnostic kit to irradiate the reagent tray; S5: And start the electric heating plate to heat the reagent tray; S6: If the reagent tray temperature is too high, the water pump and mist nozzle will cool the reagent tray; S7: Finally, the fluorescence intensity in the reagent tray will be measured by the fluorescence measurement unit.

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