Method for recovering morphology and microbial activity of anoxic ammonia oxidation granular sludge after starvation

Abstract

The present application relates to sewage treatment technical field, specifically to the method for the recovery of the morphology and microbial activity of the starved anaerobic ammonia oxidation granular sludge. The present application mixes the insoluble biochar with the particle size of 0.2-2.0 mm with the starved anaerobic ammonia oxidation granular sludge to obtain the mixture, and then inoculates the mixture into the upflow anaerobic sludge bed reactor for continuous culture under the condition of the culture medium, wherein the culture medium includes the aqueous substrate, trace elements and inorganic salts. + -N and NO2 ‑ -N, wherein the concentration of NH4 + -N is 100-150 mg N / L, the concentration of NO2 ‑ -N is 0-100 mg N / L, the concentration of NH4 + -N and NO2 ‑ -N is always greater than 1, and under this condition, the continuous culture of the starved anaerobic ammonia oxidation granular sludge can effectively recover the morphology and microbial activity of the granular sludge. The present application is simple in operation, high in feasibility, low in cost, non-toxic, and easy to apply in practical process.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of sewage treatment, and particularly relates to a method for recovering the morphology and microbial activity of starved anaerobic ammonia oxidation granular sludge. BACKGROUND

[0002] Anaerobic ammonia oxidation is a novel low-carbon and high-efficiency autotrophic biological denitrification technology. In the process, anaerobic ammonia oxidation bacteria oxidize NH4 - -N as an electron acceptor to NH4 + -N to nitrogen gas. Compared with the traditional nitrification-denitrification biological denitrification process, the anaerobic ammonia oxidation process does not require additional carbon source, and can save 40-60% of aeration energy consumption and reduce sludge production by 90%. At present, the anaerobic ammonia oxidation technology has been successfully applied in hundreds of wastewater treatment plants worldwide.

[0003] Anaerobic ammonia oxidation granular sludge, as a collection of anaerobic ammonia oxidation bacteria, provides reliable guarantee for the retention of functional microorganisms and the stable and efficient operation of the anaerobic ammonia oxidation reactor due to its high activity and granular integrity. In fact, a large amount of starved anaerobic ammonia oxidation sludge is produced in laboratories and actual wastewater treatment plants due to storage, long transportation distance, annual maintenance and sudden process shutdown of the wastewater treatment plant. The starved anaerobic ammonia oxidation sludge granules disintegrate, the biomass of anaerobic ammonia oxidation bacteria decreases, and the activity decreases, thereby causing the loss of functional microorganisms, the deterioration of denitrification effect; therefore, seeking an effective recovery method is of great significance for the rapid recovery and stable operation of the denitrification effect of the anaerobic ammonia oxidation process.

[0004] At present, some researches have explored the method for recovering the activity of starved anaerobic ammonia oxidation granular sludge. For example, for long-term starved anaerobic ammonia oxidation sludge, gradually increasing the influent substrate concentration can enhance the adaptability of microorganisms and partially recover the activity of anaerobic ammonia oxidation sludge; for example, the master's degree thesis “Research on Activity Recovery and Reuse of Starved Anaerobic Ammonia Oxidation Sludge” discloses a method for recovering the activity of anaerobic ammonia oxidation by using comet fiber fillers and honeycomb polyethylene fillers and a combination of fillers and large particle activated carbon; in batch tests, the comet fiber fillers and the honeycomb polyethylene fillers are added, and the anaerobic ammonia oxidation reaction occurs on the 8th day and the 10th day, respectively; in a continuous flow test, a self-made “honeycomb polyethylene filler and columnar large particle activated carbon” mosaic combination is added, and the reactor performance is recovered at a NO2 - -N to NH4 + -N ratio of 1.32, and the denitrification performance is recovered on the 21st day.

[0005] The above-mentioned methods can recover the activity of the anaerobic ammonia oxidation system to some extent, but the recovery effect of adjusting the influent scheme is slow, and at the same time, the cost of adding self-made fillers in actual operation is high; therefore, it is particularly important to develop an economical and efficient recovery method.

[0006] Insoluble biochar is widely available and low in price, has the characteristics of large specific surface area, strong biological compatibility, high physical and chemical stability and mechanical softness, and can be used as a microbial carrier material. Moreover, insoluble biochar contains abundant functional groups such as quinone, carboxyl, hydroxyl and amino groups, can accept electrons from electron donors, participate in the electron transfer process in the environment, and can also act as an electron acceptor for microorganisms. These characteristics are expected to promote the growth and activity recovery of anammox bacteria. Based on this, insoluble biochar can be used to recover and regulate anammox granular sludge after starvation, and an economical and efficient method for promoting the morphology repair and microbial activity recovery of anammox granular sludge after starvation is invented. SUMMARY

[0007] The purpose of the present application is to provide a method for recovering the morphology and microbial activity of anammox granular sludge after starvation, in order to solve the problem of slow recovery effect of the existing adjustment of influent scheme.

[0008] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows: a method for recovering the morphology and microbial activity of anammox granular sludge after starvation, comprising the following steps:

[0009] S1, mixing insoluble biochar with anammox granular sludge in a starvation state to obtain a mixture, wherein the particle size of the insoluble biochar is 0.2-2.0 mm;

[0010] S2, preparing a substrate aqueous solution and adding trace elements and inorganic salts to the prepared substrate aqueous solution to obtain a culture medium, wherein the concentration of NH4 + -N in the prepared substrate aqueous solution is 100-150 mg N / L, the concentration of NO2 - -N is 0-100 mg N / L, the concentration of NH4 + -N and NO2 - -N is always greater than 1;

[0011] S3, inoculating the mixture of S1 into an upflow anaerobic sludge bed reactor for continuous culture under the condition of the culture medium of S2;

[0012] S4, after a period of continuous culture, measuring the morphology and microbial activity of the anammox granular sludge.

[0013] Further, in S1, the mass ratio of insoluble biochar to anammox granular sludge in a starvation state is 0.5-2.0 g / L.

[0014] Further, in the S1, the starved anaerobic ammonia oxidation sludge is anaerobic ammonia oxidation granular sludge stored or preserved under the condition of 4-25 DEG C.

[0015] Further, in the S2, the trace elements include EDTA, FeSO4.7H2O, ZnSO4.7H2O, CoCl2.6H2O, MnCl2.4H2O, CuSO4.5H2O, NaMoO4.2H2O, NiCl2.6H2O and H3BO3.

[0016] Further, in the S2, the inorganic salts include NaH2PO4, MgSO4.7H2O, NaHCO3 and CaCl2.

[0017] Further, in the S3, after the mixture is inoculated, the concentration of suspended solids in the upflow anaerobic sludge bed reactor is 800-1500 mg / L.

[0018] Further, in the S3, the upflow anaerobic sludge bed reactor is operated in the dark, the operating temperature is 32-35 DEG C, and the hydraulic retention time is 4.0-6.5 h.

[0019] The present application has the following beneficial effects:

[0020] 1. The insoluble biochar used in the present application can be used as a nucleation carrier, and the addition of the insoluble biochar can promote the repair of the morphology of the starved anaerobic ammonia oxidation granular sludge, and the particle roundness is increased from 3.72±1.09 to 6.53±2.66, while the particle roundness of the natural recovery group without intervention is only restored to 4.22±1.38.

[0021] 2. The insoluble biochar used in the present application can be used as an extracellular electron acceptor of anaerobic ammonia oxidation bacteria and participate in the extracellular electron transfer type anaerobic ammonia oxidation process; compared with the traditional NO2 - -N and NH4 + -N, the concentration ratio of NO2 - -N is reduced appropriately in the present application, the concentration of NH4 + -N and the concentration of NO2 - -N is greater than 1, and under this condition, the anaerobic ammonia oxidation activity recovery can be accelerated; at the same time, the extracellular electron transfer type anaerobic ammonia oxidation process does not produce NO3 - -N, which can improve the denitrification efficiency of anaerobic ammonia oxidation; and in the same recovery time, the anaerobic ammonia oxidation activity of the present application is increased by 2.50-3.58 times compared with the control group.

[0022] 3. The insoluble biochar used in the present application has a wide source, is commercialized, is low in price, is environment-friendly, and is conducive to popularization. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 Specifically, the recovery of the specific anammox activity of the anammox granular sludge after starvation is shown in the following figures (a, Example 1; b, Example 2; c, Example 3; d, Example 4; e, Comparative Example 1; f, Comparative Example 2). DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be clearly and completely described in the following with reference to the accompanying drawings in the embodiments of the present application.

[0025] Example 1

[0026] The present embodiment provides a method for recovering the morphology and microbial activity of anammox granular sludge after starvation, wherein the anammox sludge in starvation state is the granular sludge stored in a laboratory refrigerator at 4℃.

[0027] The implementation of the present embodiment includes the following steps:

[0028] S1, grinding the insoluble biochar prepared from corn stalks as raw material to obtain insoluble biochar with a particle size of 0.2-1.0 mm;

[0029] S2, mixing the insoluble biochar of S1 with anammox sludge stored in a refrigerator at 4℃ for 60 days, wherein the insoluble biochar content (mass) to anammox granular sludge volume ratio is controlled to be 0.5-1.5 g / L;

[0030] S3, inoculating the mixture of S2 biochar and anammox sludge into an upflow anaerobic sludge bed reactor with an effective volume of 1.25 L for recovery test, and the suspended solid concentration in the reactor after inoculation is 800.3 mg / L; the reactor is placed in a constant temperature room at 32-35℃ for operation in the dark; the hydraulic retention time is set to 6.5 h, and is reduced by 20% every 7 days;

[0031] S4, pumping into the upflow anaerobic sludge bed reactor an influent with NH4 + -N as the substrate, and the concentration is 100 mg / L, in addition, the influent contains trace element stock solution 1.25 mL / L and inorganic salt stock solution 50 mL / L;

[0032] The composition of the trace element stock solution is as follows:

[0033] EDTA: 20 g / L; FeSO4.7H2O: 9.14 g / L; ZnSO4.7H2O: 0.43 g / L; CoCl2.6H2O: 0.24 g / L; MnCl2.4H2O: 0.99 g / L; CuSO4.5H2O: 0.25 g / L; NaMoO4.2H2O: 0.22 g / L; NiCl2.6H2O: 0.21 g / L and H3BO3: 0.014 g / L;

[0034] The composition of the inorganic salt stock solution is as follows:

[0035] NaH2PO4: 0.2 g / L; MgSO4.7H2O: 1.18 g / L; NaHCO3: 16.8 g / L; CaCl2: 0.12 g / L.

[0036] S5, another set of control experiments, inoculation of sludge is the above-mentioned starved anaerobic ammonia oxidation sludge (no addition of insoluble biochar), the other water composition and reactor running conditions are the same as above-mentioned S3~S4;

[0037] S6, after running for 14 days, the particle size change and the difference in particle roundness of the anaerobic ammonia oxidation granular sludge in the two groups of reactors (image particle size instrument) were measured, and the specific anaerobic ammonia oxidation activity was detected.

[0038] The detection results are shown in Table 1, Figure 1 a, it can be seen that the roundness recovery of the test group is obviously better than that of the control group, and the specific anaerobic ammonia oxidation activity of the test group is improved by 2.74 times compared with the control group.

[0039] Table 1 Comparison table of particle size and particle roundness recovery of anaerobic ammonia oxidation granular sludge after starvation in Example 1

[0040]

[0041] Example 2

[0042] The embodiment provides a method for recovering the morphology and microbial activity of anaerobic ammonia oxidation granular sludge after starvation, wherein the anaerobic ammonia oxidation sludge in the starvation state is taken from a breeding wastewater treatment plant that has been stopped for more than 30 days.

[0043] The implementation of the embodiment includes the following steps:

[0044] S1, screening the sludge-based biochar to obtain insoluble biochar with a particle size of 0.8-2.0 mm;

[0045] S2, mixing the insoluble biochar of S1 with the anaerobic ammonia oxidation sludge in the starvation state, and controlling the volume ratio of the content (mass) of the insoluble biochar to the anaerobic ammonia oxidation granular sludge to be 1.0-2.0 g / L;

[0046] S3, inoculate the mixture of the above-mentioned biochar and anaerobic ammonia oxidation sludge into a lift anaerobic sludge bed reactor with an effective volume of 1.25 L to carry out a recovery test, the suspended solid concentration in the reactor is 1000.7 mg / L after inoculation; the reactor is placed in a constant temperature room at 32-35℃ and runs in the dark; the hydraulic retention time is set to 6.0 h, and is reduced by 20% every 5 days;

[0047] S4, pump NH4 + -N and NO2 - -N as the substrate into the reactor, the concentration of NH4 + -N is 100 mg / L, and the concentration of NO2 - -N is 50 mg / L; in addition, the influent contains trace element stock solution 1.25 mL / L and inorganic salt stock solution 50 mL / L;

[0048] The composition of the trace element stock solution is:

[0049] EDTA: 20 g / L; FeSO4·7H2O: 9.14 g / L; ZnSO4·7H2O: 0.43 g / L; CoCl2·6H2O: 0.24 g / L; MnCl2·4H2O: 0.99 g / L; CuSO4·5H2O: 0.25 g / L; NaMoO4·2H2O: 0.22 g / L; NiCl2·6H2O: 0.21 g / L and H3BO3: 0.014 g / L;

[0050] The composition of the inorganic salt stock solution is:

[0051] NaH2PO4: 0.2 g / L; MgSO4·7H2O: 1.18 g / L; NaHCO3: 16.8 g / L; CaCl2: 0.12 g / L.

[0052] S5, another set of control experiments is set up, the inoculated sludge is the above-mentioned starved anaerobic ammonia oxidation sludge (without adding insoluble biochar), and the composition of the influent and the reactor operation conditions are the same as those in S3-S4.

[0053] S6, after the two groups of reactors are operated for 20 days, the particle size change and the difference in particle roundness of the anaerobic ammonia oxidation granular sludge in the two groups of reactors are determined, and the specific anaerobic ammonia oxidation activity is detected.

[0054] The detection results are shown in Table 2, Figure 1 b, it can be seen that the recovery of roundness in the test group is obviously better than that in the control group, and the specific anaerobic ammonia oxidation activity in the test group is increased by 3.48 times compared with that in the control group.

[0055] Table 2. Comparison of particle size and particle roundness recovery of anammox granular sludge after starvation

[0056]

[0057] Example 3

[0058] The present example provides a method for recovering the morphology and microbial activity of anammox granular sludge after starvation, wherein the anammox sludge in a starvation state is taken from an anammox reactor that has been suspended for 7 days in a laboratory.

[0059] The implementation of this example includes the following steps:

[0060] S1. Using commercially available insoluble biochar as raw material, screen to obtain insoluble biochar with a particle size of 1.0-2.0 mm;

[0061] S2. Mix the insoluble biochar with the anammox sludge in a starvation state at an insoluble biochar content (mass) to anammox granular sludge volume ratio of 0.5 g / L, 1.0 g / L, 1.5 g / L, and 2.0 g / L, respectively;

[0062] S3. Inoculate the mixture of the above biochar and anammox sludge into four groups of upflow anaerobic sludge bed reactors with an effective volume of 1.25 L for recovery tests. After inoculation, the suspended solid concentration in the reactor is 1200-1500 mg / L. According to the mixing ratio of insoluble biochar to anammox granular sludge, the four groups of reactors are labeled as R 0.5 , R 1.0 , R 1.5 , and R 2.0 , respectively. The reactors are placed in a constant temperature room at 32-35°C and run in the dark. The hydraulic retention time is set to 6.0 h, and is reduced by 20% every 5 days.

[0063] S4. Pump into the reactor influent with NH4 + -N and NO2 - -N as substrate, with NH4 + -N concentration of 150 mg / L and NO2 - -N concentration of 100 mg / L. In addition, the influent contains trace element stock solution 1.25 mL / L and inorganic salt stock solution 50 mL / L.

[0064] The composition of the trace element stock solution is:

[0065] EDTA: 20 g / L; FeSO4.7H2O: 9.14 g / L; ZnSO4.7H2O: 0.43 g / L; CoCl2.6H2O: 0.24 g / L; MnCl2.4H2O: 0.99 g / L; CuSO4.5H2O: 0.25 g / L; NaMoO4.2H2O: 0.22 g / L; NiCl2.6H2O: 0.21 g / L and H3BO3: 0.014 g / L;

[0066] The inorganic salt stock solution comprises:

[0067] NaH2PO4: 0.2 g / L; MgSO4.7H2O: 1.18 g / L; NaHCO3: 16.8 g / L; CaCl2: 0.12 g / L.

[0068] S5, another set of control experiments, inoculation of sludge is the above-mentioned starved anaerobic ammonia oxidation sludge (no addition of insoluble biochar), the other water composition and reactor operating conditions are the same as above-mentioned S3~S4;

[0069] S6, after two groups of reactors run for 20 days, the particle size change and the difference in particle roundness of anaerobic ammonia oxidation granular sludge in the five groups of reactors are measured, and the specific anaerobic ammonia oxidation activity is detected.

[0070] The detection results are shown in Table 3, Figure 1 c, it can be seen that the roundness recovery of the test group is obviously better than that of the control group, and the specific anaerobic ammonia oxidation activity of the test group is increased by 2.50~3.58 times compared with the control group.

[0071] Table 3 Comparison table of particle size and particle roundness recovery of anaerobic ammonia oxidation granular sludge after starvation in example 3

[0072]

[0073] Example 4

[0074] The embodiment provides a method for recovering the morphology and microbial activity of anaerobic ammonia oxidation granular sludge after starvation, wherein the anaerobic ammonia oxidation sludge in starvation state is taken from an anaerobic ammonia oxidation reactor which is suspended for 7 days in the laboratory.

[0075] The implementation of this embodiment includes the following steps:

[0076] S1, using commercially available insoluble biochar as raw material, sieving to obtain insoluble biochar with particle size of 0.8~1.5 mm;

[0077] S2, mixing the insoluble biochar with the anaerobic ammonia oxidation sludge in starvation state at a ratio of insoluble biochar content (mass) to anaerobic ammonia oxidation granular sludge volume of 1.2 g / L;

[0078] S3, the mixture of the above biochar and anaerobic ammonia oxidation sludge was inoculated into two groups of upflow anaerobic sludge bed reactors with an effective volume of 1.25 L for recovery test, and the suspended solid concentration in the reactor was 1300.6 mg / L after inoculation; the reactor was placed in a constant temperature room at 32-35℃ and operated in the dark; the hydraulic retention time was set to 4.0 h;

[0079] S4, NH4 + -N and NO2 - -N as the substrate was pumped into the reactor, the initial concentration of NH4 + -N was 100 mg / L, the initial concentration of NO2 - -N was 50 mg / L, and the concentration of NH4 + -N and NO2 - -N in the influent was adjusted every 5 days with an increase of 25 mg / L; in addition, the influent contained trace element stock solution 1.25 mL / L and inorganic salt stock solution 50 mL / L;

[0080] The composition of the trace element stock solution is:

[0081] EDTA: 20 g / L; FeSO4·7H2O: 9.14 g / L; ZnSO4·7H2O: 0.43 g / L; CoCl2·6H2O: 0.24 g / L; MnCl2·4H2O: 0.99 g / L; CuSO4·5H2O: 0.25 g / L; NaMoO4·2H2O: 0.22 g / L; NiCl2·6H2O: 0.21 g / L and H3BO3: 0.014 g / L;

[0082] The composition of the inorganic salt stock solution is:

[0083] NaH2PO4: 0.2 g / L; MgSO4·7H2O: 1.18 g / L; NaHCO3: 16.8 g / L; CaCl2: 0.12 g / L.

[0084] S5, another set of control experiments was set up, the inoculated sludge was the above-mentioned starved anaerobic ammonia oxidation sludge (without adding insoluble biochar), and the other compositions of the influent and the reactor operation conditions were the same as those in S3-S4 above;

[0085] S6, after the two groups of reactors were operated for 14 days, the particle size change and the difference in particle roundness of the anaerobic ammonia oxidation granular sludge in the two groups of reactors were determined, and the specific anaerobic ammonia oxidation activity was detected.

[0086] The detection results are shown in Table 4, Figure 1As shown in Fig. d, it can be seen that the recovery of the roundness of the test group is obviously better than that of the control group, and the anaerobic ammonia oxidation activity of the test group is improved by 2.93 times compared with the control group.

[0087] Table 4 Comparison of particle size and recovery of particle roundness of anaerobic ammonia oxidation granular sludge after starvation in Example 4

[0088]

[0089] Comparative Example 1

[0090] The c(NH4 + -N):c(NO2 - -N) of the matrix composition of the present comparative example is 1:1.32, and no biochar is added, and the other operation conditions are the same as those of Example 4, and the specific conditions are as follows: the anaerobic ammonia oxidation sludge in a starved state used is the same as that of Example 4, i.e., taken from the anaerobic ammonia oxidation reactor which has been suspended for 7 days in the laboratory.

[0091] The implementation of the present comparative example includes the following steps:

[0092] S1, inoculate the above-mentioned anaerobic ammonia oxidation sludge in a starved state into a UASB reactor with an effective volume of 1.25 L for recovery test, and the suspended solid concentration in the reactor is 1302.3 mg / L after inoculation; place the reactor in a constant temperature room at 32-35°C for operation in the dark; and set the hydraulic retention time to 4.0 h;

[0093] S2, pump the influent with NH4 + -N and NO2 - -N as the substrate into the reactor, and the initial concentration of NH4 + -N is 100 mg / L, and the initial concentration of NO2 - -N is 132 mg / L; adjust the concentration of NH4 + -N in the influent by an increment of 25 mg / L every 5 days, and appropriately adjust the concentration of NO2 - -N in the influent to maintain c(NH4 + -N):c(NO2 - -N) of the influent at 1:1.32; in addition, the influent contains trace element stock solution 1.25 mL / L and inorganic salt stock solution 50 mL / L;

[0094] The composition of the trace element stock solution is as follows:

[0095] EDTA: 20 g / L; FeSO4·7H2O: 9.14 g / L; ZnSO4·7H2O: 0.43 g / L; CoCl2·6H2O: 0.24g / L; MnCl2·4H2O: 0.99 g / L; CuSO4·5H2O: 0.25 g / L; NaMoO4·2H2O: 0.22 g / L; NiCl2·6H2O: 0.21 g / L and H3BO3: 0.014 g / L;

[0096] The composition of the inorganic salt stock solution is as follows:

[0097] NaH2PO4: 0.2 g / L; MgSO4·7H2O: 1.18 g / L; NaHCO3: 16.8 g / L; CaCl2: 0.12 g / L.

[0098] S3. After the reactor has been running for 14 days, the changes in particle size and particle roundness of the anaerobic ammonia oxidation granular sludge in the reactor are measured, and the specific anaerobic ammonia oxidation activity is detected.

[0099] The test results are shown in Table 5. Figure 1 As shown in e, the particle size and roundness of Comparative Example 1 were not significantly different from those of the control group in Example 4; the specific anaerobic ammonia oxidation activity was 1.35 ± 0.31 mgN / (g VSS h). Figure 1 e), the activity recovery was not significantly different compared with the control group in Example 4.

[0100] Table 5 Comparison of particle size and sphericity recovery of anaerobic ammonia oxidation granular sludge after starvation in Comparative Example 1

[0101]

[0102] Comparative Example 2

[0103] In this comparative example, the matrix composition contains c(NH4) + -N):c(NO2 - -N)=1:1.32, and other operating conditions are the same as in Example 4, as follows: The starved anaerobic ammonia oxidation sludge used is the same as in Example 4, that is, it is taken from the anaerobic ammonia oxidation reactor that has been suspended from operation for 7 days in the laboratory.

[0104] The implementation of this comparison includes the following steps:

[0105] S1. Using commercially available insoluble biochar as raw material, insoluble biochar with a particle size of 0.8~1.5 mm is obtained by sieving.

[0106] S2. Mix insoluble biochar with starved anaerobic ammonia oxidation sludge at a ratio of insoluble biochar content (mass) to anaerobic ammonia oxidation granular sludge volume of 1.2 g / L.

[0107] S3, inoculate the mixture of the above biochar and anaerobic ammonia oxidation sludge into a upflow anaerobic sludge bed reactor with an effective volume of 1.25 L for recovery test, the suspended solid concentration in the reactor is 1304.1 mg / L after inoculation; the reactor is placed in a constant temperature room at 32-35℃ and runs in the dark; the hydraulic retention time is set to 4.0 h;

[0108] S4, pump NH4 + -N and NO2 - -N as the substrate into the reactor, the initial concentration of NH4 + -N is 100 mg / L, the initial concentration of NO2 - -N is 132 mg / L, adjust the concentration of NH4 + -N in the influent every 5 days with an increment of 25 mg / L, and appropriately adjust the concentration of NO2 - -N in the influent to maintain the ratio of c(NH4 + -N) to c(NO2 - -N) at 1:1.32; in addition, the influent contains trace element stock solution 1.25 mL / L and inorganic salt stock solution 50 mL / L;

[0109] The composition of the trace element stock solution is:

[0110] EDTA: 20 g / L; FeSO4·7H2O: 9.14 g / L; ZnSO4·7H2O: 0.43 g / L; CoCl2·6H2O: 0.24 g / L; MnCl2·4H2O: 0.99 g / L; CuSO4·5H2O: 0.25 g / L; NaMoO4·2H2O: 0.22 g / L; NiCl2·6H2O: 0.21 g / L and H3BO3: 0.014 g / L;

[0111] The composition of the inorganic salt stock solution is:

[0112] NaH2PO4: 0.2 g / L; MgSO4·7H2O: 1.18 g / L; NaHCO3: 16.8 g / L; CaCl2: 0.12 g / L.

[0113] S5, after 14 days of reactor operation, measure the particle size change and particle roundness difference of the anaerobic ammonia oxidation granular sludge in the reactor, and detect the specific anaerobic ammonia oxidation activity.

[0114] The detection results are shown in Table 6, Figure 1 f, the particle size and particle roundness of Comparative Example 2 have no obvious difference compared with the control group of Example 4; the specific anaerobic ammonia oxidation activity is 1.58±0.39 mgN / (g VSS h)Figure 1 f) the difference in recovery of activity was not significant compared to the control of Example 4.

[0115] Table 6 Recovery of particle size and roundness of anaerobic ammonium oxidation granular sludge after starvation of Comparative Example 2

[0116]

[0117] The present application is not limited to the above-mentioned best mode, and anyone can derive other various forms of products under the inspiration of the present application, but regardless of any change in shape or structure, any technical solution with the same or similar to the present application falls within the scope of the present application.

Claims

1. A method for recovering the morphology and microbial activity of anammox granular sludge after starvation, characterized in that, The method comprises the following steps: S1, mixing insoluble biochar and starved anaerobic ammonia oxidation granular sludge to obtain a mixture, wherein the particle size of the insoluble biochar is 0.2-2.0 mm; The starved anaerobic ammonia oxidation sludge is anaerobic ammonia oxidation granular sludge stored or preserved at 4-25℃; S2, a base solution is prepared, and trace elements and inorganic salts are added to the prepared base solution to obtain a culture medium; wherein the concentration of NH4 + -N is 100 mg / L, the concentration of NO2 - -N is 50 mg / L, or the concentration of NH4 + -N is 150 mg / L, the concentration of NO2 - -N is 100 mg / L; the concentration of NH4 + -N and the concentration of NO2 - The concentration ratio of NH4 + N to NO2 - N is always greater than 1, and under this condition, the recovery of anaerobic ammonia oxidation activity can be accelerated. S3, inoculating the mixture of S1 into an upflow anaerobic sludge bed reactor under the culture medium of S2 for continuous culture; S4, after continuous culture for a period of time, determining the morphology and microbial activity of the anaerobic ammonia oxidation granular sludge.

2. The method for recovering the morphology and microbial activity of starved anammox granular sludge according to claim 1, characterized in that: In S1, the mass / volume ratio of the insoluble biochar to the starved anaerobic ammonia oxidation granular sludge is 0.5-2.0 g / L.

3. The method for recovering the morphology and microbial activity of starved anammox granular sludge according to claim 1, characterized in that: In S2, the trace elements include EDTA, FeSO4·7H2O, ZnSO4·7H2O, CoCl2·6H2O, MnCl2·4H2O, CuSO4·5H2O, NaMoO4·2H2O, NiCl2·6H2O and H3BO3.

4. The method for recovering the morphology and microbial activity of starved anammox granular sludge according to claim 1, characterized in that: In S2, the inorganic salts include NaH2PO4, MgSO4·7H2O, NaHCO3 and CaCl2.

5. The method for recovering the morphology and microbial activity of starved anammox granular sludge according to claim 1, characterized in that: In S3, after inoculation of the mixture, the concentration of suspended solids in the upflow anaerobic sludge bed reactor is 800-1500 mg / L.

6. The method for recovering the morphology and microbial activity of starved anammox granular sludge according to claim 1, characterized in that: In S3, the upflow anaerobic sludge bed reactor is operated in the dark, the operating temperature is 32-35℃, and the hydraulic retention time is 4.0-6.5 h.

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