A plant-based compound composition for inhibiting Propionibacterium acnes, its application in nanogels, and preparation methods.

Abstract

This invention discloses a plant-based compound composition for inhibiting Propionibacterium acnes, its application in nanogels, and a preparation method, relating to the cosmetics field. The plant-based compound composition for inhibiting Propionibacterium acnes includes matrine, magnolol, taurine, and artemisinin essential oil. This application combines four traditional Chinese medicine ingredients—matrine, magnolol, taurine, and artemisinin essential oil—based on traditional Chinese medicine theory, exhibiting a synergistic effect. Compared to single-ingredient traditional Chinese medicine components, this combination offers superior antibacterial and acne-removing efficacy, and the side effects of traditional Chinese medicine ingredients are lower than those of chemical acne treatments. Furthermore, the plant-based compound composition for inhibiting Propionibacterium acnes is encapsulated into a nanogel, which enhances the antibacterial activity and storage stability of the traditional Chinese medicine components. This nanogel has less oil content, making it easier to absorb. The prepared nanogel features uniform particle size distribution and small particle size, facilitating drug penetration and absorption on the skin surface and improving drug utilization efficiency.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and more particularly to a plant-based compound composition for inhibiting Propionibacterium acnes, a nanogel for its application, and a method for its preparation. Background Technology

[0002] Acne, commonly known as "pimples," is one of the most common skin problems. It is a chronic inflammatory skin disease affecting the sebaceous glands, mainly manifested as comedones, papules, pustules, nodules, and cysts. Acne is prevalent among adolescents, closely related to their high sebum secretion and oily skin. However, in recent years, the incidence and severity of acne in adults have increased. Acne most commonly occurs on the face, easily leaving post-inflammatory hyperpigmentation, causing patients to lack confidence in their appearance and seriously affecting their physical and mental health. Therefore, acne has received increasing attention from the cosmetics industry, and acne-removing products have become best-selling cosmetics. Consumers hoping to solve their acne problems with acne-removing products usually want immediate results and require products that are fast-acting, reliable, and long-lasting.

[0003] Currently, there are many types of raw materials or active ingredients used for acne treatment, mainly including salicylic acid, squalane, urea, and tea tree oil. These raw materials or active ingredients can help improve skin problems to some extent, but they also have some shortcomings. Some raw materials are only effective for specific types or degrees of acne, and there are also issues such as side effects and drug resistance; moreover, there are individual differences, and different people have different cure rates due to individual characteristics and environmental influences. For example, salicylic acid is a chemical component with antibacterial and keratolytic properties, which can effectively remove dirt and dead skin cells from pores. However, excessive or long-term use of cosmetics containing salicylic acid may lead to problems such as dry skin and irritation, and it is not suitable for people with sensitive skin.

[0004] Traditional Chinese medicine (TCM) has a wide application in acne treatment using cosmetics. As a traditional treatment method, TCM has accumulated rich experience and achievements in the field of acne treatment. The antibacterial active ingredients in TCM can achieve acne-reducing effects by reducing bacterial growth. However, these active ingredients sometimes have insufficient antibacterial ability or a continuously declining antibacterial activity, resulting in the effects of TCM acne treatment not being rapid or significant, requiring a long and slow treatment course. Furthermore, long-term use of antibacterial products may lead to skin dependence on their antibacterial abilities; if the use of such products is suddenly stopped, bacterial rebound and proliferation may occur.

[0005] Therefore, there is a need to develop a new and effective raw material for acne-removing cosmetics that meets the requirements of high acne-removing and antibacterial capabilities and the maintenance of antibacterial activity, and to support the application of this new raw material in downstream products, thereby further enriching the cosmetic raw material market. Summary of the Invention

[0006] This invention provides a plant-based compound composition for inhibiting Propionibacterium acnes, a nanogel for its application, and a preparation method thereof, in order to solve the problems of insufficient antibacterial properties, long treatment time, and easy side effects of acne-removing raw materials currently used in cosmetics.

[0007] To address the aforementioned technical problems, one objective of this invention is to provide a plant-based compound composition for inhibiting Propionibacterium acnes, comprising the following components in parts by weight:

[0008] Matrine: 3-4.8 parts;

[0009] And magnolol: 3-5 parts;

[0010] Peach tannin: 1-2 parts;

[0011] Artemisia annua essential oil: 1-2 parts.

[0012] As a preferred embodiment, it also includes 20-40 parts by weight of water and 10-30 parts by weight of organic solvent.

[0013] As a preferred embodiment, the organic solvent is propylene glycol.

[0014] To address the aforementioned technical problems, a second objective of this invention is to provide a method for preparing a plant-based compound composition for inhibiting Propionibacterium acnes, comprising the following steps:

[0015] (1) Dissolve matrine in water to obtain an aqueous solution of matrine;

[0016] (2) Dissolve magnolol and taurin in an organic solvent to obtain a mixed solution;

[0017] (3) Weigh out Artemisia annua essential oil and Matrine aqueous solution, mix them evenly to obtain a plant compound composition for inhibiting Propionibacterium acnes.

[0018] By adopting the above-mentioned scheme, this application combines four traditional Chinese medicine components—matrine, magnolol, taurine, and artemisia annua oil—and extracts these components using organic solvents. Matrine can inhibit the growth of Propionibacterium acnes, suppress the release of inflammatory mediators, and stabilize cell membranes. It can also activate adenylate cyclase on the cell membrane, thereby increasing intracellular cAMP and calcium ion levels, leading to a cascade of immune amplification reactions and achieving anti-inflammatory and anti-allergic effects. Artemisia annua extract can inhibit TSLP and promote FLG gene expression, repairing the skin, strengthening the skin barrier, and exerting antibacterial and anti-inflammatory effects. Taurine, by altering cell membrane integrity and permeability, has antioxidant and acne-reducing effects. It can also improve the skin microenvironment by inhibiting the growth of Gram-negative bacteria such as Propionibacterium acnes and Staphylococcus aureus, reducing and inhibiting acne formation. Magnolioside exhibits significant antibacterial activity against Gram-positive bacteria, acid-fast bacteria, and filamentous fungi, with a more pronounced antibacterial effect against Streptococcus mutans and the strongest inhibitory effect against Staphylococcus aureus. The various herbal components exhibit synergistic effects, resulting in a more effective antibacterial and acne-reducing composition compared to single herbal ingredients. Furthermore, the antibacterial and acne-reducing active ingredients from these herbs have less skin irritation and fewer side effects compared to chemical raw materials, demonstrating higher safety.

[0019] To address the aforementioned technical problems, a third objective of this invention is to provide a plant-based compound composition nanogel for inhibiting Propionibacterium acnes, comprising the following components by mass fraction:

[0020] Plant-based compound acne treatment composition: 0.5-2.5 wt%;

[0021] Propylene glycol: 10-14 wt%;

[0022] Carbomer: 0.5-1.2 wt%;

[0023] pH adjuster: 0.5-1.2 wt%;

[0024] Hydrogenated lecithin: 0.8-1.2 wt%;

[0025] Caprylic / capric triglycerides: 2-5 wt%;

[0026] Preservative: 0.4-0.6 wt%;

[0027] Corrosion inhibitor: 0.4-0.6 wt%

[0028] Water: Balance.

[0029] As a preferred embodiment, the pH adjuster is triethanolamine.

[0030] As a preferred embodiment, the preservative is p-hydroxyacetophenone.

[0031] As a preferred embodiment, the corrosion inhibitor is 1,2-hexanediol.

[0032] As a preferred embodiment, the carbomer is at least one of carbomer 940, carbomer 934, carbomer 941, carbomer 970 and carbomer 980.

[0033] To address the aforementioned technical problems, the fourth objective of this invention is to provide a method for preparing a plant-based compound composition nanogel for inhibiting Propionibacterium acnes, comprising the following steps:

[0034] (1) Add carbomer to water and stir at room temperature for 1-3 hours to dissolve it, thus obtaining gel phase A;

[0035] (2) Weigh out caprylic / capric triglyceride and pour it into a beaker. Control the water bath temperature at 30-50℃. Add hydrogenated lecithin and dissolve it completely. Continue stirring for 1-3 hours to obtain organic phase B.

[0036] (3) Add gel phase A, organic phase B, and plant compound composition for inhibiting Propionibacterium acnes into a container and disperse using a high-speed dispersion device at a speed of 2000-10000 rpm / min for 15-40 min. Control the water bath temperature at 60-70℃, add preservatives and preservative synergists, and after the temperature drops to room temperature, add pH adjuster to adjust the pH to 4.5-5.5. Mix to obtain nanogel.

[0037] By adopting the above scheme, this application uses encapsulation technology to prepare a nanogel from a plant compound composition for inhibiting Propionibacterium acnes. This can improve the dispersibility of the composition, while also enhancing the antibacterial activity and storage stability of the traditional Chinese medicine ingredients. The prepared nanogel has the characteristics of uniform particle size distribution and small particle size, which helps the drug penetrate and absorb on the skin surface, improves drug utilization efficiency, and further promotes the acne-removing effect.

[0038] To address the aforementioned technical problems, the fifth objective of this invention is to provide an application of a plant-based compound composition for inhibiting Propionibacterium acnes or a plant-based compound composition nanogel for inhibiting Propionibacterium acnes in the field of cosmetic preparation.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] 1. This application analyzes the mechanism of acne development and formulates a prescription based on traditional Chinese medicine theory, combining four Chinese herbal ingredients: matrine, magnolol, tau tannin, and artemisia annua essential oil. These ingredients have a synergistic effect, and the combined composition has better antibacterial and acne-removing effects than single Chinese herbal ingredients. Furthermore, the antibacterial and acne-removing active ingredients of traditional Chinese medicine have less irritation to the skin and fewer side effects than chemical acne-removing raw materials, thus exhibiting higher safety.

[0041] 2. This application uses a plant-based compound composition for inhibiting Propionibacterium acnes to prepare a nanogel using encapsulation technology. This can improve the dispersibility of the composition, while also enhancing the antibacterial activity and storage stability of the traditional Chinese medicine ingredients. The nanogel has less oil content and is easier to absorb. The prepared nanogel has the characteristics of uniform particle size distribution and small particle size, which helps the drug penetrate and absorb on the skin surface, improves drug utilization efficiency, further promotes the acne-removing effect, and enriches the raw materials for cosmetic acne removal. Attached Figure Description

[0042] Figure 1 This is a schematic diagram illustrating the preparation process of a plant-based compound composition for inhibiting Propionibacterium acnes in the preparation examples of this invention.

[0043] Figure 2 Photographs of the inhibition zone test of the plant compound composition for inhibiting Propionibacterium acnes in Example 1 of this invention (Note: the upper left is the composition of Example 1, the upper right is deionized water, the lower left is propylene glycol, and the lower right is 0.1% piodin).

[0044] Figure 3 Photographs of the inhibition zone test of the plant compound composition for inhibiting Propionibacterium acnes in Example 2 of this invention (Note: the upper left is the composition of Example 2, the upper right is deionized water, the lower left is propylene glycol, and the lower right is 0.1% piracetam).

[0045] Figure 4 This is a schematic diagram illustrating the preparation process of the plant-based compound composition for inhibiting Propionibacterium acnes nanogel in this embodiment of the invention.

[0046] Figure 5 : The particle size test results of the plant compound composition for inhibiting Propionibacterium acnes nanogel in Example 1 of this invention;

[0047] Figure 6 : The particle size test results of the plant compound composition for inhibiting Propionibacterium acnes nanogel in Example 2 of the present invention;

[0048] Figure 7 : The particle size test results of the plant compound composition for inhibiting Propionibacterium acnes nanogel in Example 3 of the present invention;

[0049] Figure 8 : The particle size test results of the plant compound composition for inhibiting Propionibacterium acnes nanogel in Example 4 of this invention;

[0050] Figure 9 : The particle size test results of the plant compound composition for inhibiting Propionibacterium acnes in Comparative Example 1 of this invention are shown.

[0051] Figure 10 : The particle size test results of the plant compound composition for inhibiting Propionibacterium acnes in Comparative Example 2 of this invention are shown.

[0052] Figure 11 : This shows the particle size test results of the plant compound composition for inhibiting Propionibacterium acnes nanogel in Comparative Example 3 of this invention. Detailed Implementation

[0053] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0054] Table 1 - Names and sources of raw materials and equipment used in the embodiments and comparative examples of this application

[0055]

[0056]

[0057] Preparation Examples 1-6 and Comparative Preparation Examples 1-10

[0058] A plant-based compound composition for inhibiting Propionibacterium acnes includes matrine, magnolol, terpineol, artemisinin essential oil, propylene glycol, and water. The components and their contents are shown in Table 2 below. Figure 1 As shown, the preparation method includes the following steps: dissolving matrine in 30g of purified water to obtain a matrine aqueous solution; dissolving magnolol and terpineol in 20g of propylene glycol to obtain a mixed solution; weighing artemisia annua essential oil and mixing it evenly with the above matrine aqueous solution and mixed solution to obtain a plant compound composition for inhibiting Propionibacterium acnes.

[0059] Comparative Preparation Example 11

[0060] A plant-based compound composition for inhibiting Propionibacterium acnes includes matrine, magnolol, taurine, artemisia annua oil, propylene glycol, and water. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving matrine in 30g of purified water to obtain a matrine aqueous solution; dissolving magnolol and taurine in 20g of propylene glycol to obtain a mixed solution; weighing artemisia annua oil and mixing it evenly with the above-mentioned matrine aqueous solution and mixed solution to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0061] Comparative preparation example 12

[0062] A plant-based compound composition for inhibiting Propionibacterium acnes includes matrine, magnolol, paeonol, artemisia annua oil, propylene glycol, and water. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving matrine in 30g of purified water to obtain a matrine aqueous solution; dissolving magnolol and paeonol in 20g of propylene glycol to obtain a mixed solution; weighing artemisia annua oil and mixing it evenly with the above-mentioned matrine aqueous solution and mixed solution to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0063] Comparative preparation example 13

[0064] A plant-based compound composition for inhibiting Propionibacterium acnes includes matrine, magnolol, taurine, cinnamon oil, propylene glycol, and water. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving matrine in 30g of purified water to obtain an aqueous matrine solution; dissolving magnolol and taurine in 20g of propylene glycol to obtain a mixed solution; weighing cinnamon oil and mixing it evenly with the above-mentioned matrine aqueous solution and mixed solution to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0065] Comparative preparation example 14

[0066] A plant-based compound composition for inhibiting Propionibacterium acnes includes oxymatrine, magnolol, taurine, artemisia annua oil, propylene glycol, and water. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving oxymatrine in 30g of purified water to obtain an aqueous solution of oxymatrine; dissolving magnolol and taurine in 20g of propylene glycol to obtain a mixed solution; weighing artemisia annua oil and mixing it evenly with the above-mentioned aqueous solution of oxymatrine and the mixed solution to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0067] Comparative preparation example 15

[0068] A plant-based compound composition for inhibiting Propionibacterium acnes includes matrine, magnolol, paeonol, artemisia annua oil, propylene glycol, and water. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving matrine in 30g of purified water to obtain a matrine aqueous solution; dissolving magnolol and paeonol in 20g of propylene glycol to obtain a mixed solution; weighing artemisia annua oil and mixing it evenly with the above-mentioned matrine aqueous solution and mixed solution to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0069] Comparative Preparation Example 16

[0070] A plant-based compound composition for inhibiting Propionibacterium acnes includes oxymatrine and purified water. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving oxymatrine in 50g of purified water to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0071] Comparative preparation example 17

[0072] A plant-based compound composition for inhibiting Propionibacterium acnes includes magnolol and propylene glycol. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving magnolol in 50g of propylene glycol to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0073] Comparative Preparation Example 18

[0074] A plant-based compound composition for inhibiting Propionibacterium acnes includes paeonol and propylene glycol. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: dissolving paeonol in 50g of propylene glycol to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0075] Comparative preparation example 19

[0076] A plant-based compound composition for inhibiting Propionibacterium acnes includes cinnamon oil, propylene glycol, and purified water. The components and their contents are shown in Table 2 below. The preparation method includes the following steps: mixing cinnamon oil with 30g of purified water and 20g of propylene glycol until homogeneous to obtain the plant-based compound composition for inhibiting Propionibacterium acnes.

[0077] Table 2 - Components and contents of the plant-based compound composition for inhibiting Propionibacterium acnes in the preparation examples and comparative preparation examples of this application

[0078]

[0079]

[0080]

[0081] Performance testing

[0082] 1. The MIC of Propionibacterium acnes in the plant-based compound composition for inhibiting Propionibacterium acnes:

[0083] Table 3 - Test Materials and Equipment

[0084] name batch supplier Propionibacterium acnes ATCC 11827 Guangdong Provincial Microbial Culture Collection Center (GDMCC) Enhanced Clostridium culture medium Cat#LA4360 Beijing Solarbio Technology Co., Ltd. Agar powder S27282 Shanghai Yuanye Biotechnology Co., Ltd. Constant temperature and humidity chamber DZF-6020 Shanghai Yiheng Scientific Instruments Co., Ltd. Clean bench SW-CJ-2D Zhejiang Fuxia Medical Technology Co., Ltd.

[0085] Propionibacterium acnes stock culture was inoculated onto reinforced Clostridium tumefaciens medium (RCM) and incubated at 37°C for activation. A small amount of bacterial colony was picked and diluted to 0.5 McFarland units with sterile physiological saline to prepare an inoculum suspension. The suspension was sealed, refrigerated, and stored for later use. One drug concentration was measured per row. The MIC values ​​of the plant-based compound extracts for inhibiting Propionibacterium acnes in the prepared and control prepared examples were determined using 2 mL EP tubes.

[0086] The experiment was conducted using the microdilution method. 500 μL of culture medium was added to all 2 mL EP tubes. Then, 500 μL of the plant-based compound inhibitory composition for *Propionibacterium acnes* from either the preparation or control preparation was added to well 1. 500 μL of the mixture was then added to well 2, and so on, up to well 12, resulting in drug dilutions of 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024, 2048, and 4096 times. 500 μL of the drug solution was discarded from well 12. Then, 500 μL of inoculum suspension was added to wells 1-13, with well 13 being the growth group and well 14 being the blank group. After culturing at 37°C for 24 hours, the growth of *Propionibacterium acnes* was observed. The lowest dilution concentration at which no bacterial growth occurred was defined as the minimum inhibitory concentration (MIC). The results are shown in Table 4 below.

[0087] Table 4 - Minimum inhibitory concentrations of plant-based compound compositions inhibiting Propionibacterium acnes in the preparation examples and comparative preparation examples of this application

[0088] Testing items MIC (μg / mL) Testing items MIC (μg / mL) Preparation Example 1 1.32 Comparative Preparation Example 8 87.55 Preparation Example 2 2.55 Comparative preparation example 9 15.75 Preparation Example 3 3.08 Comparative Preparation Example 10 36.75 Preparation Example 4 2.85 Comparative Preparation Example 11 38.25 Preparation Example 5 1.89 Comparative preparation example 12 27.70 Preparation Example 6 2.25 Comparative preparation example 13 21.50 Comparative Preparation Example 1 20.50 Comparative preparation example 14 45.93 Comparative Preparation Example 2 3.48 Comparative preparation example 15 87.75 Comparative preparation example 3 62.50 Comparative Preparation Example 16 40.77 Comparative preparation example 4 125.00 Comparative preparation example 17 25.68 Comparative preparation example 5 25.57 Comparative Preparation Example 18 51.25 Comparative preparation example 6 105.55 Comparative preparation example 19 225.39 Comparative preparation example 7 65.28 - -

[0089] 2. Synergistic index of plant-based compound composition inhibiting Propionibacterium acnes against Propionibacterium acnes:

[0090] Combination Index (CI): CI is an important parameter for measuring whether a combination of compounds has a synergistic or antagonistic effect. The Chou-Talalay model is a commonly used pharmacological model for evaluating the synergistic and antagonistic effects of compound combinations during the onset of action. The calculated CI results are shown in Table 5 below.

[0091] The synergy index (CI) is calculated using the Chou-Talalay formula, which is as follows:

[0092]

[0093] Where C a C b C c C d These represent the concentrations corresponding to the antibacterial effects of components A, B, C, and D acting individually; C A C B C C C D C represents the inhibitory effect observed at corresponding concentrations when the individual components are combined into a composition. A-D Result = MIC experimental data of composition × C A-D The proportion of the component in the composition.

[0094] After calculating the CI value, its magnitude is used to interpret the effects of the drug combination on the organism:

[0095] (1) If CI < 1, it indicates that there is a synergistic effect. In this case, it means that the combination of components has a stronger or more significant effect on improving the target physiological process or therapeutic efficacy than the individual components used alone.

[0096] (2) If CI = 1, it means that there is no interaction. In this case, it means that the components do not exert any additional gain or loss.

[0097] (3) If CI > 1, it indicates an antagonistic effect. In this case, it means that the combination of components shows a lower or weaker significant improvement in the target physiological process or therapeutic efficacy compared to the individual components used alone. Table 5 - Synergistic effect index of the plant compound composition for inhibiting Propionibacterium acnes in the preparation examples and comparative preparation examples of this application.

[0098]

[0099]

[0100] 3. Inhibition zone test of plant-based compound composition for inhibiting Propionibacterium acnes:

[0101] The plant-based compound composition for inhibiting Propionibacterium acnes, tea tree oil, and piracetam from both the preparation and control examples were appropriately diluted in deionized water to prepare MIC solutions with concentrations at least two orders of magnitude higher (100-fold). Propionibacterium acnes was cultured on RCM agar plates. Small circular paper discs (6.0 mm in diameter) were planted on the plates, and 10 μL of the plant-based compound composition for inhibiting Propionibacterium acnes, 10 μL of deionized water (blank control), 10 μL of tea tree oil (positive control), and 10 μL of... were added to the discs respectively. 0.1% piocin (positive control) and 10 μL propylene glycol (negative control) were incubated at 37°C for 24 h and 48 h, respectively. The diameter of the inhibition zone was measured. Each concentration of each bacterium was tested in triplicate (diameter > 7 mm and p < 0.05, indicating statistical significance). The results are shown in Table 6 below. Photographs of the inhibition zones in Examples 1-2 are shown below. Figure 2-3 As shown.

[0102] Table 6 - Results of the inhibition zones of the plant-based compound compositions for inhibiting Propionibacterium acnes in the preparation examples and comparative preparation examples of this application.

[0103]

[0104]

[0105] A comparison of the antibacterial test results in Tables 4-6 shows that this application targets the mechanism of acne development, using traditional Chinese medicine theory to formulate a composition that inhibits Propionibacterium acnes. Matrine inhibits the growth of Propionibacterium acnes, suppresses the release of inflammatory mediators, stabilizes cell membranes, and activates adenylate cyclase on the cell membrane, thereby increasing intracellular cAMP and calcium ion levels, leading to a cascade of immune amplification reactions and achieving anti-inflammatory and anti-allergic effects. Artemisia annua extract inhibits TSLP and promotes FLG gene expression, repairing the skin, strengthening the skin barrier, and exerting antibacterial and anti-inflammatory effects. Prunol alters cell membrane integrity and permeability, exhibiting antioxidant and acne-reducing effects. It also improves the skin microenvironment by inhibiting the growth of Gram-negative bacteria such as Propionibacterium acnes and Staphylococcus aureus, reducing and inhibiting acne formation. Magnolioside exhibits significant antibacterial activity against Gram-positive bacteria, acid-fast bacteria, and filamentous fungi, with a more pronounced antibacterial effect against Streptococcus mutans and the strongest inhibitory effect against Staphylococcus aureus. The components of this herbal compound composition for inhibiting Propionibacterium acnes exhibit synergistic effects, demonstrating higher antibacterial activity and better acne-removing efficacy compared to individual herbal ingredients.

[0106] Example 1

[0107] A plant-based compound composition for inhibiting Propionibacterium acnes is disclosed as a nanogel comprising 2 wt% of the plant-based compound composition for inhibiting Propionibacterium acnes from Preparation Example 1, 12 wt% propylene glycol, 1 wt% carbomer 940, 1 wt% triethanolamine, 1 wt% hydrogenated lecithin, 3 wt% caprylic / capric triglyceride (GTCC), 0.5 wt% p-hydroxyacetophenone, 0.5 wt% 1,2-hexanediol, and the balance being purified water. Figure 4 As shown, its preparation method includes the following steps:

[0108] (1) Add Carbomer 940 to purified water and stir at room temperature for 1 hour to dissolve it, thus obtaining gel phase A;

[0109] (2) Weigh out caprylic / capric triglyceride and pour it into a beaker. Control the water bath temperature at 40°C. Add hydrogenated lecithin. After the hydrogenated lecithin is completely dissolved, continue stirring for 1 hour to obtain organic phase B.

[0110] (3) Add gel phase A, organic phase B and plant compound composition for inhibiting Propionibacterium acnes into a container, disperse with a high-speed disperser, mix evenly, at a speed of 6000 rpm / min, disperse for 15 min, add p-hydroxyacetophenone and 1,2-hexanediol at a water bath temperature of 60℃, and after the sample temperature drops to room temperature, add triethanolamine to adjust the pH to 5.0, mix to obtain nanogel.

[0111] Example 2

[0112] A plant-based compound nanogel for inhibiting Propionibacterium acnes is prepared in the same way as that in Example 1, with the same steps, reagents and process parameters. The difference is that in step (3), the speed of the high-speed disperser is 2000 rpm / min.

[0113] Example 3

[0114] A plant-based compound composition for inhibiting Propionibacterium acnes nanogel is prepared in the same way as Example 1, except that in step (3), the speed of the high-speed disperser is 10000 rpm / min.

[0115] Example 4

[0116] A plant-based compound nanogel for inhibiting Propionibacterium acnes is prepared in the same way as that in Example 1, with the same steps, reagents and process parameters. The difference is that in step (3), the dispersion time of the high-speed disperser is 30 min.

[0117] Comparative Example 1

[0118] A plant-based compound composition for inhibiting Propionibacterium acnes nanogel is prepared in the same way as Example 1, except that the dispersion time of the high-speed disperser in step (3) is 5 min.

[0119] Comparative Example 2

[0120] A plant-based compound nanogel for inhibiting Propionibacterium acnes is prepared in the same way as that in Example 1, with each step, reagent and process parameter being the same. The difference is that hydrogenated lecithin is replaced by an equal amount of glyceryl stearate.

[0121] Comparative Example 3

[0122] A plant-based compound nanogel for inhibiting Propionibacterium acnes is prepared using the same steps, reagents, and process parameters as in Example 1, except that caprylic / capric triglyceride is replaced by polydimethylsiloxane in equal amounts.

[0123] Performance testing

[0124] 1. Particle size determination: Take 2g of the nanogels from Examples 1-4 and Comparative Examples 1-3 into a 10mL centrifuge tube, add 2g of deionized water, shake well, and pipette 0.1mL of the supernatant into a 10mL volumetric flask. Dilute to the mark with 0.9% sodium chloride aqueous solution to obtain the test solution. Measure the particle size using a nanoparticle size analyzer. The test results are shown in Table 7 below. Figure 1-11 As shown.

[0125] Table 7 - Particle size test results of nanogels in Examples 1-4 and Comparative Examples 1-3

[0126] Test Project Particle size (mm) PDI Example 1 698.6 0.228 Example 2 656.8 0.291 Example 3 630.1 0.389 Example 4 638.6 0.334 Comparative Example 1 973.5 0.565 Comparative Example 2 1395.2 0.930 Comparative Example 3 693.2 0.864

[0127] As shown in Table 7, this application uses nano-encapsulation technology to prepare a plant-based compound composition for inhibiting Propionibacterium acnes into a nanogel, which can improve the storage stability and antibacterial activity of the plant-based compound composition for inhibiting Propionibacterium acnes. The particle size distribution of the encapsulated composition is uniform and the particle size is small, which helps the drug penetrate deep into the skin surface, helps the drug absorption, improves the acne removal effect, and achieves rapid acne removal.

[0128] 2. Stability Test:

[0129] 1) The nanogel samples prepared in Example 1 were centrifuged at 3000 r / min for 30 min. No separation was observed after centrifugation.

[0130] 2) Long-term stability test of the nanogel sample prepared in Example 1: The sample was placed under high temperature (40℃), low temperature (-15℃), high humidity (RH 90%, 25℃) and strong light ((4500±500) lx) conditions for 30 days, and the test results were observed on the 0, 5, 10 and 30 days of the test. The test results are shown in Table 8 below.

[0131] The preservative performance was tested using the culture medium method. Staphylococcus aureus is a common bacteria prone to spoilage, so a sterile culture medium suitable for its growth was used to observe its preservative performance. Staphylococcus epidermidis and Escherichia coli are also common spoilage bacteria, and their preservative performance was observed using sterile nutrient agar medium suitable for their growth. Nutrient agar medium and agar medium suitable for Staphylococcus aureus growth were prepared and set aside. Using aseptic techniques, 0.5g of the prepared gel sample was inoculated into the two prepared culture media (nutrient agar medium and Staphylococcus aureus medium) and incubated at 37℃. The presence of visible bacterial or fungal colonies was observed on the two agar plates at 24h and 48h. If no colonies were observed to the naked eye, the condition was considered good.

[0132] The pH value was tested using a dilution method at a concentration of 10%.

[0133] Table 8 - Stability test results of Example 1 under different environmental conditions on day 30

[0134]

[0135] 3. Consumer User Experience Survey: The nanogel prepared in Example 1 was used for a consumer survey test. 33 participants were selected, including 5 males and 28 females, aged 21-35 years. Inclusion criteria were facial skin lesions, primarily acne, with a small number of pustules or papules, totaling 10-50 lesions. The test site was the entire face. Participants used the product continuously for 14 days, twice daily (morning and evening). A questionnaire survey was conducted, and the final results are shown in Table 9 below. The participants' satisfaction with the product was analyzed. Satisfaction = Number of satisfactory evaluations / Total number of participants. Satisfaction evaluations included very satisfied, fairly satisfied, and somewhat satisfied. No adverse reactions occurred during the experiment, and no participants withdrew from the test.

[0136] Table 9 - Consumer Survey Results of Example 1

[0137]

[0138]

[0139] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the scope of protection of the present invention. In particular, it should be noted that any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention for those skilled in the art.

Claims

1. A plant-based compound composition for inhibiting Propionibacterium acnes, characterized in that, Includes the following components by weight: Matrine: 3-4.8 parts; And magnolol: 3-5 parts; Peach tannin: 1-2 parts; Artemisia annua essential oil: 1-2 parts.

2. The plant-based compound composition for inhibiting Propionibacterium acnes as described in claim 1, characterized in that, It also includes 20-40 parts by weight of water and 10-30 parts by weight of organic solvent.

3. The plant-based compound composition for inhibiting Propionibacterium acnes as described in claim 2, characterized in that, The organic solvent is propylene glycol.

4. A method for preparing a plant-based compound composition for inhibiting Propionibacterium acnes as described in claim 2 or 3, characterized in that, Includes the following steps: (1) Dissolve matrine in water to obtain an aqueous solution of matrine; (2) Dissolve magnolol and terpineol in an organic solvent to obtain a mixed solution; (3) Weigh out Artemisia annua essential oil and Matrine aqueous solution, mix them evenly to obtain a plant compound composition for inhibiting Propionibacterium acnes.

5. A plant-based compound nanogel for inhibiting Propionibacterium acnes, characterized in that, The plant-based compound composition for inhibiting Propionibacterium acnes as described in any one of claims 1-3 comprises the following components by mass fraction: Plant-based compound composition for inhibiting Propionibacterium acnes: 0.5-2.5 wt%; Propylene glycol: 10-14 wt% Carbomer: 0.5-1.2 wt%; pH adjuster: 0.5-1.2 wt%; Hydrogenated lecithin: 0.8-1.2 wt%; Caprylic / capric triglycerides: 2-5 wt% Preservative: 0.4-0.6 wt%; Anti-corrosion synergist: 0.4-0.6wt% Water: Balance.

6. The plant-based compound nanogel for inhibiting Propionibacterium acnes as described in claim 5, characterized in that, The pH adjuster is triethanolamine.

7. The plant-based compound nanogel for inhibiting Propionibacterium acnes as described in claim 5, characterized in that, The preservative is p-hydroxyacetophenone.

8. The plant-based compound nanogel for inhibiting Propionibacterium acnes as described in claim 5, characterized in that, The corrosion inhibitor is 1,2-hexanediol.

9. A method for preparing a plant-based compound composition nanogel for inhibiting Propionibacterium acnes as described in any one of claims 5-8, characterized in that, Includes the following steps: (1) Add carbomer to water and stir at room temperature for 1-3 h to dissolve it, thus obtaining gel phase A; (2) Weigh out caprylic / capric triglyceride and pour it into a beaker. Control the water bath temperature at 30-50℃. Add hydrogenated lecithin and dissolve it completely. Continue stirring for 1-3 h to obtain organic phase B. (3) Add gel phase A, organic phase B and plant compound acne-removing composition to a container and disperse using a high-speed dispersion device at a speed of 2000-10000 rpm for 15-40 min. Control the water bath temperature at 60-70℃, add preservatives and preservative synergists, and after the temperature drops to room temperature, add pH adjuster to adjust the pH to 4.5-5.

5. Mix to obtain nano gel.

10. The application of a plant-based compound composition for inhibiting Propionibacterium acnes or a plant-based compound composition for inhibiting Propionibacterium acnes nanogel as described in any one of claims 1-3 and 5-8 in the preparation of cosmetics.

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